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Academic literature on the topic 'Antibody-Libraries'

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Published: 1 April 2023

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Journal articles on the topic "Antibody-Libraries"

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Vaughan, T. J., A. J. Williams, K. Pritchard, J. K. Osbourn, A. R. Pope, J. C. Earnshaw, J. McCafferty, J. Wilton, and K. S. Johnson. "Human antibody libraries." Immunotechnology 2, no. 1 (February 1996): 72–73. http://dx.doi.org/10.1016/1380-2933(96)80683-x.

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Valadon, Philippe, Sonia M. Pérez-Tapia, Renae S. Nelson, Omar U. Guzmán-Bringas, Hugo I. Arrieta-Oliva, Keyla M. Gómez-Castellano, Mary Ann Pohl, and Juan C. Almagro. "ALTHEA Gold Libraries™: antibody libraries for therapeutic antibody discovery." mAbs 11, no. 3 (February 26, 2019): 516–31. http://dx.doi.org/10.1080/19420862.2019.1571879.

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Lou, Kai-Jye. "Fab libraries for antibody discovery." Science-Business eXchange 3, no. 44 (November 2010): 1314. http://dx.doi.org/10.1038/scibx.2010.1314.

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Benhar, Itai. "Design of synthetic antibody libraries." Expert Opinion on Biological Therapy 7, no. 5 (May 2007): 763–79. http://dx.doi.org/10.1517/14712598.7.5.763.

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Bradbury, Andrew R. M., and James D. Marks. "Antibodies from phage antibody libraries." Journal of Immunological Methods 290, no. 1-2 (July 2004): 29–49. http://dx.doi.org/10.1016/j.jim.2004.04.007.

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Rader, Christoph, and Carlos F. Barbas. "Phage display of combinatorial antibody libraries." Current Opinion in Biotechnology 8, no. 4 (August 1997): 503–8. http://dx.doi.org/10.1016/s0958-1669(97)80075-4.

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Little, Melvyn, Frank Breitling, Stefan Dübel, Patrick Fuchs, and Michael Braunagel. "Human antibody libraries in Escherichia coli." Journal of Biotechnology 41, no. 2-3 (July 1995): 187–95. http://dx.doi.org/10.1016/0168-1656(95)00022-i.

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Zhou, Heyue, Yan-Liang Zhang, Guodi Lu, Henry Ji, and Charles P. Rodi. "Recombinant antibody libraries and selection technologies." New Biotechnology 28, no. 5 (September 2011): 448–52. http://dx.doi.org/10.1016/j.nbt.2011.03.013.

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Hoogenboom, Hennie R. "Selecting and screening recombinant antibody libraries." Nature Biotechnology 23, no. 9 (September 2005): 1105–16. http://dx.doi.org/10.1038/nbt1126.

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Catcott, Kalli C., Molly A. McShea, Carl Uli Bialucha, Kathy L. Miller, Stuart W. Hicks, Parmita Saxena, Thomas G. Gesner, et al. "Microscale screening of antibody libraries as maytansinoid antibody-drug conjugates." mAbs 8, no. 3 (January 11, 2016): 513–23. http://dx.doi.org/10.1080/19420862.2015.1134408.

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Dissertations / Theses on the topic "Antibody-Libraries"

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Charlton, Keith Alan. "Combinatorial antibody display libraries from sheep and their analysis." Thesis, University of Aberdeen, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342711.

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Büssow, Konrad. "Arrayed cDNA libraries for antibody screening and systematic analysis of gene products." [S.l. : s.n.], 1998. http://www.diss.fu-berlin.de/1999/53/index.html.

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Skamaki, Kalliopi. "In vitro evolution of antibody affinity using libraries with insertions and deletions." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/286439.

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In Nature, antibodies are capable of recognizing a huge variety of different molecular structures on the surface of antigens. The primary factor that defines the structural diversity of the antibody antigen combining site is the length variation of the complementarity determining region (CDR) loops. Following antigen stimulation, further diversification through the process called somatic hypermutation (SHM) leads to antibodies with improved affinity and specificity. Sequence diversification by SHM is mainly achieved by introduction of point substitutions and a small percentage of insertions/deletions (indels). Although the percentage of indels in affinity matured antibodies is low, probably due to the low rate incorporation of in-frame indels throughout the course of the SHM diversification process, it is likely that the antibody fold can accommodate higher diversity of affinity-enhancing indels. By in vitro evolution, other researchers have sampled either only restricted diversity of indels or extended diversity of insertions only in specific positions chosen based on structural information and natural length variation. The aim of this thesis was to study the impact of random and high diversity indels on antibody affinity by in vitro evolution. New approaches for construction of libraries with in-frame amino acid indels were applied to enable sampling of indels of different lengths across the entire antibody variable domains. I followed two different approaches for construction of indel libraries. Firstly, a recently developed random approach allowed the construction of libraries with random insertions and deletions. Secondly, a semi-random approach was developed to build libraries with different lengths of insertions that could be widely applied in future in vitro antibody affinity maturation campaigns. Libraries constructed by either of these approaches yielded variants with insertions with improved affinity. Overall, this thesis demonstrates that insertions besides offering alternative routes to affinity maturation can also be combined with point substitutions to take advantage of additive effects on function.
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Sblattero, Daniele. "The Development of a Single Vector Recombination System to Make Large Phage Antibody Libraries." Doctoral thesis, SISSA, 1999. http://hdl.handle.net/20.500.11767/4385.

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1.1.1 The aim of the reasearch Phage display has been recently introduced as a means of making antibodies in vitro(Barbas, Kang et al. 1991; Griffiths, Malmqvist et al. 1993; Griffiths, Williams et al. 1994; Vaughan, Williams et al. 1996; Sheets, Amersdorfer et al. 1998). In general, the affinity of the antibodies isolated is pfoportional to the initial size of the library used for selection. This is based on both theoretical (Perelson and Oster 1979) and practical (Vaughan, Williams et al. 1996) considerations. In view of this, the creation of larger and larger libraries has bec01ne an important goal in the use of this technology in the selection of antibodies against any antigen. The aim of my research were: - the design and the validation of a new phagemid vector for phage display of antibody fragments (scFvs). - The design of a new strategy for "one vector" in viva recombination. - The construction of a large naive scFv libray 1.1.2 Results obtained The following chapters will describe all the steps taken in order to achieve the final goal: the construction of a large phage antibody library. First of all there will be the description of the design of a new phagemid vector in which all the possible variables were considered from a theoretical point of view, and the best elements were chosen for use. Secondly data confirming the quality of the vector will be pre_sent.ed. __ This was achieved first with the cloning of several mAb and then with the construction of two small immune libraries that allow the selection of several specific and functional scFv. The third part will describe a method which uses a single vector to exploit the reversibility of ere catalysed recombination. This method involves the creation of a relatively small primary library (7xl o7 was used here) in a phagemid vector in which the VH and VL genes are separated by two nonhomologous lox sites. The heavy and light chain genes in this primary library are then recombined by infecting the phagemid particles into ere expressing bacteria at high multiplicity of infection (MOI). Under these conditions many different phagemid particles enter a single bacteria and the VH and VL genes are exchanged between different phagemids, creating many new VH/VL combinations, all of which are functional. This ends with the production of a large naive library of preaviously unattainable size that was validated by the selection of antibodies, with high affinity, against a large number of different protein antigens. In order to fully appreciate the different aspects of experimental works and its underlying strategies some theoretical paragraphs are included in this first chapter.
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Prendergast, D. "Discovery of tumour necrosis factor receptor-1 (p55) binding peptides using a phage display library." Thesis, Queen's University Belfast, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368468.

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Persic, Lidija. "The selection of specific phage antibodies from phage antibody libraries and their expression in different systems." Thesis, Open University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299004.

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Chiliza, Thamsanqa Emmanuel. "Antibody phage-displayed libraries derived from chicken immunoglobulin genes a source of highly specific diagnostic antibodies /." Diss., Pretoria : [s.n.], 2007. http://upetd.up.ac.za/thesis/available/etd-07012008-080736/.

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Alturki, Norah. "Expression of Biotinylated Multivalent Peptide Antigens in Bacteria for Rapid and Effective Generation of Single Domain Antibodies from Phage-displayed Antibody Libraries." Thesis, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/23523.

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In the present study, two insulin-like growth factor-binding protein 7 (IGFBP7) C-terminal-peptides were expressed as fusion proteins to bacterial verotoxin pentamerization domain as shown by Western blotting, ELISA and mass spectroscopy. Both in vivo-biotinylated recombinant products were purified from bacterial lysates by IMAC and used directly for panning along with the recombinant IGFBP7 protein using the LAC-M Camelidae naïve single domain antibody (sdAb) library. Target-specific sdAbs to both parental protein and peptide fusions were identified by phage ELISA. Twelve different clones were isolated by phage-ELISA screening and their sdAb genes were sequenced. Soluble sdAbs and their pentameric formats were expressed in TG1 E. coli, purified by IMAC and characterized by ELISA and SPR. Several sdAbs are currently under study, however anti-IGFBP7 (P12/M12) was extensively characterized and exhibited promising anti-tumorigenic effect on PANC-1 cell lines by blocking IGFBP7 promoting activity. This study provides the basis for developing a novel imaging/therapeutic reagent for targeting and treating brain tumor angiogenesis in early stages of tumorogenesis and can also be used as a molecular tool to monitor the degree of angiogenesis in gliomas which may help to improve the clinical management of brain tumors.
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Diebolder, Philipp [Verfasser], and Roland E. [Akademischer Betreuer] Kontermann. "Generation of "LYmph Node Derived Antibody Libraries" (LYNDAL) : a concept for recovering human monoclonal antibodies with therapeutic potential / Philipp Diebolder ; Betreuer: Roland E. Kontermann." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2014. http://d-nb.info/112545069X/34.

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Giudice, Anna Maria <1991&gt. "ABC transporters of the A subfamily: potential prognostic markers and candidates for antibody selection from phage-display libraries for therapeutic use in Ewing sarcoma." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2020. http://amsdottorato.unibo.it/9421/1/Giudice_AnnaMaria_tesi.pdf.

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The prognostic value of ABC transporters in Ewing sarcoma is still poorly explored and controversial. We described for the first time the impact of various ABCs on Ewing sarcoma prognosis by assessment of their gene expression in two independent cohorts of patients. Unexpected associations with favourable outcomes were observed for two ABCs of the A-subfamily, ABCA6 and ABCA7, whereas no associations with the canonical multidrug ABC transporters were identified. The ABCs of the A-subfamily are involved in cholesterol/phospholipids transportation and efflux from cells. Our clinical data support the drug-efflux independent contribution to cancer progression of the ABCAs, which has been confirmed in PDX-derived cell lines. The impact of these ABCA transporters on tumor progression seems to be mediated by lowering intracellular cholesterol, supporting the role of these proteins in lipid transport. In addition, the gene expression of ABCA6 and ABCA7 is regulated by transcription factors which control lipid metabolism: ABCA6 was induced by the binding of FoxO1/FoxO3a to its promoter and repressed by IGF1R/Akt signaling, whereas the expression of ABCA7 was regulated by p53. The data point to ABCA6 and ABCA7 as potential prognostic markers in Ewing sarcoma and suggest the IGF1/ABCA/lipid axis as an intriguing therapeutic target. Agonist monoclonal antibodies towards ABCA6/7 or inhibitors of cholesterol biosynthesis, such as statins or aminobiphoshonates, may be investigated as therapeutic options in combination with chemotherapy. Considering that no monoclonal antibodies selectively targeting extracellular domains of ABCA6/7 are available, the second part of the project has been dedicated to the generation of human antibody phage-display libraries as tools for selecting monoclonal antibodies. A novel synthetic human antibody phage-display library has been designed, cloned and characterized. The library takes advantages of the high variability of a designed naïve repertoire to be a useful tool for isolating antibodies towards all potential antigens, including the ABCAs.
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Book chapters on the topic "Antibody-Libraries"

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Martineau, Pierre. "Synthetic Antibody Libraries." In Antibody Engineering, 85–97. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-01144-3_6.

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Nelson, Bryce, and Sachdev S. Sidhu. "Synthetic Antibody Libraries." In Methods in Molecular Biology, 27–41. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-921-1_2.

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Hust, Michael, and Stefan Dübel. "Human Antibody Gene Libraries." In Antibody Engineering, 65–84. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-01144-3_5.

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Simmen, Frank A., Denis R. Headon, Tanya Z. Schulz, Mohan Cope, David A. Wright, Graham Carpenter, and Bert W. O’Malley. "Antibody-Screening cDNA Libraries." In Investigation and Exploitation of Antibody Combining Sites, 83–90. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-5006-4_7.

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Caucheteur, Déborah, Gautier Robin, Vincent Parez, and Pierre Martineau. "Construction of Synthetic Antibody Libraries." In Antibody Engineering, 93–108. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8648-4_5.

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Hutchings, Catherine, Sara Carmen, and Simon Lennard. "Generation of Naive Human Antibody Libraries." In Antibody Engineering, 93–108. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-662-04605-0_6.

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Fehrsen, Jeanni, Susan Wemmer, and Wouter van Wyngaardt. "Construction of Chicken Antibody Libraries." In Methods in Molecular Biology, 189–203. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7447-4_10.

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Peng, Hung-Pin, and An-Suei Yang. "Computational Analysis of Antibody Paratopes for Antibody Sequences in Antibody Libraries." In Computer-Aided Antibody Design, 437–45. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2609-2_24.

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Pasello, Michela, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, and Katia Scotlandi. "Construction of Human Naïve Antibody Gene Libraries." In Antibody Engineering, 73–91. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8648-4_4.

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Hust, Michael, André Frenzel, Torsten Meyer, Thomas Schirrmann, and Stefan Dübel. "Construction of Human Naive Antibody Gene Libraries." In Antibody Engineering, 85–107. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-974-7_5.

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Conference papers on the topic "Antibody-Libraries"

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LERNER, RICHARD, JIA XIE, HONGKAI ZHANG, KYUNGMOO YEA, JOEL BLANCHARD, and KRISTIN BALDWIN. "REGULATING CELLULAR LIFE DEATH AND DEVELOPMENT USING INTRACELLULAR COMBINATORIAL ANTIBODY LIBRARIES." In 23rd International Solvay Conference on Chemistry. WORLD SCIENTIFIC, 2014. http://dx.doi.org/10.1142/9789814603836_0037.

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Schmidt, A., K. Becker-Peters, A. Orlowski, and C. Königs. "Isolation of vWF-specific antibody fragments from phage-displayed scFv libraries." In GTH Congress 2023 – 67th Annual Meeting of the Society of Thrombosis and Haemostasis Research – The patient as a benchmark. Georg Thieme Verlag, 2023. http://dx.doi.org/10.1055/s-0042-1760565.

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Kim, Duwoon, Seok-Ryel Kim, Donal F. Day, and Myung-Joo Oh. "A Novel Screening Method of Dextran Binding Antibody Using Phage Display Libraries." In 2007 Frontiers in the Convergence of Bioscience and Information Technologies. IEEE, 2007. http://dx.doi.org/10.1109/fbit.2007.73.

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Bjorklid, E., T. Johansen, U. R. Pendurthi, L. V. M. Rao, B. Warn-Cramer, and S. T. Rapaport. "HUMAN cDNA CLONES FOR THROMBOPLASTIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643735.

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A rabbit polyclonal antibody which was monospecific for thromboplastin (TP) apoprotein from human brain according to a number of criteria was used to screen two human placenta cDNA libraries in the expression vector λgt11, one randomly primed and one oligo-dT primed, by the method of Young and Davis. 23 positive clones expressing TP related antigen were isolated and plaque purified. DNA from the different clones was isolated and the TPcDNA inserts released by EcoRl digestion. The inserts could be classified into11 size classes ranging from approx. 300-1100 base pairs.The largest insert (1100 bp) was subcloned intothe plasmid vector pGEM-1. When the nick-translated plasmid (pTP4-l) was used as a probe to screen the phage clones by slot blot hybridization all the 23clones hybridized to the 1100 bp insert. A λgt11TP4 lysogen expressed β-galactosidase- TP4 fusion peptide upon IPTG induction as shown by immunobinging studied using two different antibodies to TP apoprotein: the rabbit antibody originally used to screen the libraries and an antibody raised in goat against human brain TP purified by affinity chromatography on a Factor VII-antiVII-agarose column.
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Uzan, G., A. Lajmanovich, M. H. Prandini, Ph Frachet, A. Duperray, and G. Marguerie. "MOLECULAR CLONING OF PLATELET GPIIb FROM HEL CELLS AND HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643960.

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Platelet GP IIb-IIIa is an heterodimer which functions as a receptor for fibrinogen, fibronectin and Von Willebrand factor and is implicated in platelet adhesive reactions. To study the structure function relationship of this glycoprotein, a recombinant DNA approach was initiated. cDNA expression libraries were constructed in » gtll vector, from erythro-leukemia cells (HEL) and megakaryocytes mRNA. The human megakaryocytes were isolated from patients with chronic myeloid leukemia. The HEL library was initially screened with polyclonal antibodies anti GPIIb IIIa. One clone, λIIbI, containing a 1.65 kbp insert reacted with a panel of different polyclonal antibodies anti GPIIb IIIa and a monoclonal antibody anti GPIIb. To further characterize this clone the synthesis of the fusion protein was induced by IPTG. The bacterial protein was then blotted onto nitro cellulose and incubated with antisera anti GPIIb-IIIa. Antibodies that specifically bound with the fusion protein were eluted and tested on platelet membrane extracts. The selected antibodies produced a positive signal at the GPIIb position similar to the signal produced by the monoclonal antibody anti GPIIb on the same membrane extract. Finally on western blotting, a protein of Mr= 170kD reacted with the monoclonal antibody anti GPIIb. λIIbI insert was used to screen the megakaryocyte library and 3 clones, λIIb2,λIIb3 and λIIb4 were isolated. The size of HEL cells and megakaryocytes GPIIb mRNA was estimated by northern blotting. Only one species of 3.9 kb was identified in both cells. The four different clones accounted for 50% of the coding sequence of this mRNA.Sequencing of these cDNAs indicated that the plasmatic domain of GPIIb contains a cystein rich region. The sequence of these clones will allow the study of the adhesines genetic diversity in different cellular systems.
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Carlsen, E., and H. Prydz. "ROLE OF BIOLOGICAL RESPONSE MODIFIERSIN THE REGULATION OF THROMBOPLASTIN SYNTHESIS IN MONOCYTES AND ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643736.

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A rabbit polyclonal antibody which was monospecific for thromboplastin (TP) apoprotein from human brain according to a number of criteria was used to screen two human placenta cDNA libraries in the expression vector Agtll, one randomly primed and one oligo-dT primed, by the method of Young and Davis. 23 positive clones expressing TP related antigen were isolated and plaque purified. DNA from the different clones was isolated and the TPcDNA inserts released by EcoRl digestion. The inserts could be classified into11 size classes ranging from approx. 300-1100 base pairs.The largest insert (1100 bp) was subcloned intothe plasmid vector pGEM-1. When the nick-translated plasmid (pTP4-l) was used as a probe to screen the phage clones by slot blot hybridization all the 23clones hybridized to the 1100 bp insert. A XgtllTP4 lysogen expressed B-galactosidase- TP4 fusion peptide upon IPTG induction as shown by immunobinging studied using two different antibodies to TP apoprotein: the rabbit antibody originally used to screen the libraries and an antibody raised in goat against human brain TP purified by affinity chromatography on a Factor Vll-antiVII-agarose column.Cytokines mediate many of the cellular interactions in the inflammatory and immune response systems and have a variety of actions. We have investigated the effect of rIL-1a/$,rIL-2, rlFNa/y and rTNFa on thromboplastin synthesis (TPL) in monocytes (M) and human umbilical vein endothelial cells (HUVEC). Recombinant IL- 1a and IL-16 both induced a dose dependent increase in TPL activity of monocyte (8-fold) and HUVEC cultures (15-fold) at 6h. The increase levelled off at interleukinconcentrations of 50-100u/ml. Recombinant IL-2 at 50u/ml induced a 5-fold rise in monocytes TPL. The effect of rIL-2 on HUVEC TPL synthesis at 6 h was smaller than on monocytes but still clearly significant at dose dependent. Recombinant IFN-Y (10 -10 u/ml) increased.TPL activity in HUVEC at 6h and 16 h in adose dependent manner, whereas no effect of rIFN-Y and IFN-a (1-10 u/ml) on M TPL was seen. When LPS (5pg/ml) was used to induce TPL synthesis, additional stimulation with rIFN-Y further enhanced HUVEC TPL activity, but decreased M TPL activity. Recombinant IFNa also decreased LPS induced TPL synthesis in M andhad no effect on HUVEC TPL. Recombinant TNFa (0.3x104u/ml) increased HUVEC TPL 7-fold at 6h. There wasno effect on M TPL synthesis. No endotoxin was detected in any of these preparations. CONCLUSIONS: Some biological response modifiers induced thromboplastin synthesis in monocytes (IL-ia, IL-13, IL-2) and in human umbilical vein endothelial cells (IL-ia, IL-18, IL-2, TNFa and IFNY). Some had no direct effect on TPL synthesis but inhibited the response to monocytes to other thromboplastin-inducing agents like LPS (IFNa and IFNY).
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Reports on the topic "Antibody-Libraries"

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Sharon, Jacqueline. Recombinant Polyclonal Antibody Libraries for Breast Cancer Therapy. Fort Belvoir, VA: Defense Technical Information Center, September 2000. http://dx.doi.org/10.21236/ada397043.

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Lillo, Antonietta, Geoffrey Waldo, Nileena Velappan, and Hau Nguyen. Development of COVID19 antibody cocktails by in vitro evolution of display libraries. Office of Scientific and Technical Information (OSTI), January 2021. http://dx.doi.org/10.2172/1760553.

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Benkovic, Stephen J. The Use of Combinatorial Heavy and Light Chain Libraries and Site Specific Mutagenesis to Create Antibody Biosensors for Metal Ions. Fort Belvoir, VA: Defense Technical Information Center, April 1997. http://dx.doi.org/10.21236/ada325575.

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Benkovic, Stephen J. The Use of Combinatorial Heavy and Light Chain Libraries and Site Specific Mutagenesis to Create Antibody Biosensors for Metal Ions. Fort Belvoir, VA: Defense Technical Information Center, May 1992. http://dx.doi.org/10.21236/ada252020.

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