Journal articles: 'Anti-jam'

1

Casabona, Mario M., and Murray W. Rosen. "Discussion of GPS Anti-Jam Technology." GPS Solutions 2, no. 3 (January 1999): 18–23. http://dx.doi.org/10.1007/pl00012752.

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2

Fairhurst, Godred, and Tim Spracklen. "An anti-jam packet data satellite link." International Journal of Satellite Communications 7, no. 3 (July 1989): 201–7. http://dx.doi.org/10.1002/sat.4600070310.

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3

Ariyanti, Ariyanti, Eni Masruriati, Tsani Imadahidayah, and Eka Nur Sulistianingsih. "Pemanfaatan kitosan dari cangkang kerang bulu (Anadara antiquata) sebagai pengawet ikan pari (Dasyatis sp.) dan udang vaname (Litopenaeus vannamei)." Riset Informasi Kesehatan 9, no. 1 (June 29, 2020): 12. http://dx.doi.org/10.30644/rik.v9i1.241.

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Latar Belakang : Penggunaan senyawa anti mikroba yang tepat dapat memperpanjang umur simpan suatu produk serta menjamin keamanan produk. Untuk itu dibutuhkan bahan sebagai anti mikroba yang alami supaya tidak membahayakan bagi kesehatan. Penggunaan kitosan untuk menghambat aktivitas mikroba pada udang vaname (Litopenaeus vannamei) akan diuji efektivitasnya. Metode : Pada penelitian ini kitosan yang digunakan sebagai anti mikroba diekstraksi dari cangkang kerang bulu (Anadara antiquata). Kitosan yang diperoleh kemudian digunakan sebagai anti mikroba ikan pari (Dasyatis sp.) dan udang vaname (Litopenaeus vannamei). Kitosan dilarutkan dalam asam asetat dengan variasi konsentrasi kitosan 1%; 1,5%, 2% dan 2,5%. Lama waktu penyimpanan udang: 0 jam, 5 jam, 10 jam, 15 jam dan 20 jam. Hasil : Kitosan dari cangkang kerang bulu dapat digunakan sebagai pengawet alami ikan pari dan udang vaname. Konsentrasi optimal kitosan yang digunakan sebagai pengawet ikan pari adalah 2% dapat memperpanjang umur simpan ikan selama 15 jam. Sedangkan konsentrasi optimal kitosan yang digunakan sebagai pengawet udang vaname adalah 1,5% dapat memperpanjang umur simpan ikan selama 15 jam. Kesimpulan : Konsentrasi kitosan cangkang kerang bulu yang paling optimal sebagai pengawet alami ikan pari adalah 2% dapat memperpanjang umur simpan ikan pari selama 15 jam.

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4

Nemov, A. V., D. A. Nemov, D. L. Minh, S. A. Saveliev, and A. N. Plastikov. "Efficiency of the Miniature Anti-jam GNSS Antenna Array." Journal of the Russian Universities. Radioelectronics, no. 2 (June 5, 2018): 37–46. http://dx.doi.org/10.32603/1993-8985-2018-21-2-37-46.

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The use of the miniature controlled reception pattern antennas (CRPAs) in GNSS equipment is one of the trends in GPS, Baidow, GLONASS development. A miniaturized GPS antenna array technology reduces the size of the antenna elements and the array dimensions. Miniature CRPAs are in demand not only with mass consumer of GPS/GLONASS house-hold equipment, but with expert users of complex hardware as well, where high-tech multi-sensor miniature antenna systems (AC) can be applied. Such types of AC used for intelligent control of spatial selectivity are considered as antenna arrays. The advantages of miniature CRPAs with anti-jamming capability include possibility to be installed on vehicles where it used to be impossible due to their size. The negative effect of miniaturization is in degradation of some antennas characteristics, such as gain, suppression of the reverse lobe of radiation pattern (RP), a heterogeneity of RP. In miniature antennas, the resonator interinfluence increases, that leads to distortion of individual emitters RP and to the in-crease of the total RP lobe of the antenna array irregularity, as well as the width of RP lobe. Designers take special measures to reduce the interinfluence of the resonators. However, they are not fully described in the available literature. Therefore, the achieved performance of miniature CRPAs is in great interest. The final criterion (from a consumer point of view) is in effective functional of a device containing a miniature CRPA, the degradation of its parameters in compare with traditional CRPA equipment of expert users. The authors focus on property investigation of miniature CRPAs manufactured primarily by US industry. Specifications of two antennas and some expected details of the miniaturized antenna array technology are described along with the test results of their ability to perform the objective function jammer suppression. The article contains the results obtained from independent testing of electrodynamics parameters of miniature L1/L2 frequency CRPA and its design analysis. The experimental data of sensor interinfluence are outlined. The measures to reduce the sensor interinfluence are take into account. The efficiency of the miniature antenna is estimated in the process of interference suppression by means of computer simulation. The Monte-Carlo method is applied. For the sake of generality, two types of algorithm for interference suppression are used.

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5

Dhiancinantyan Windydaca Brata Putri and Ni Putu Aryati Suryaningsih. "EFEK EKSTRAK DAUN SENDOK (Plantago major L.) TERHADAP ERITEMA PADA MARMUT PUTIH BETINA (Guinea pig) OLEH RADIASI ALAT MODIFIKASI UV 04-08." Jurnal Ilmiah Medicamento 4, no. 1 (March 30, 2018): 1–12. http://dx.doi.org/10.36733/medicamento.v4i1.872.

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Penelitian ini bertujuan untuk mengetahui efek anti-inflamasi ekstrak daun sendok (Plantago major L.) pada hewan coba berupa marmut putih betina (Guinea pig) yang diinduksi radiasi UV-B (290-320 nm). Penelitian ini menggunakan 30 ekor marmut putih betina yang dibagi menjadi kelompok uji sebanyak 10 ekor, kelompok pembanding sebanyak 10 ekor dan kelompok kontrol sebanyak 10 ekor. Kelompok uji diberi kapsul daun sendok dengan dosis 50 mg/kg yang diberikan secara oral tiap 5 jam sekali sehari, kelompok pembanding diberi obat antalgin dengan dosis 50 mg/kg yang diberikan secara oral tiap 5 jam sekali sehari dan kelompok kontrol diberi aquadem secara oral tiap 5 jam sekali sehari. Parameter yang diamati berupa perbandingan gradasi eritema dan persentase perubahan luas area eritema setelah diinduksi radiasi UV-B dan 24 jam setelah diinduksi radiasi UV-B. Berdasarkan hasil persentase perubahan luas area eritema dan gradasi eritema dapat disimpukan bahwa ekstrak daun sendok memiliki efek anti-inflamasi, tetapi efek anti-inflamasi ekstrak daun sendok tidak sebaik efek anti-inflamasi antalgin (sebagai pembanding).

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6

Byarushengo, Denice, Rwaichi Minja, and Abraham Temu. "Lemongrass and Cinnamon Essential Oils as Vitamin C Preservatives and Flavour Enhancers in Jam." Tanzania Journal of Engineering and Technology 35, no. 1 (June 30, 2014): 46–53. http://dx.doi.org/10.52339/tjet.v35i1.468.

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Lemongrass and cinnamon essential oils (LEO and CEO) are natural oils with high anti-oxidation capacity, pleasant flavour, and various health benefits. Their ability to preservevitamin C and flavour in jam have not been tested. In this study the performance of twoessential oils (EOs) in preserving vitamin C and improving jam flavour were investigated.The EOs were produced by hydro-steam distillation of fresh lemongrasses and cinnamonleaves using a Clevenger apparatus. Jam samples were dosed with various concentrationsof either single or mixed EOs and then stored at either room or refrigeration temperature.Samples were analysed for changes in vitamin C content and flavour, after every 10 daysfor 60 days. Vitamin C content was determined using 2,6 dichlorophenol indophenol visualtitration method, whereas sensory analysis was done by five semi trained panellists. It wasrevealed that both LEO and CEO have high potential to reduce loss of vitamin C andimpart better flavour in pineapple jam. The improved quality of the jam is due to anti-microbial and anti-oxidant effects of the essential oils as reported in literature. Mixing theEOs had synergistic effect which maximizes their potential to reduce vitamin C loss withlower dose than when used individually. Mixed EOs doses also enhanced the jam flavour.

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Martynyuk, S. Ye, D. O. Vasylenko, F. F. Dubrovka, and A. G. Laush. "Novel microstrip antenna array for anti-jam satellite navigation system." Radioelectronics and Communications Systems 58, no. 3 (March 2015): 97–106. http://dx.doi.org/10.3103/s0735272715030012.

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8

Fairhurst, G. "On-board processing for anti-jam packet data satcom services." Electronics & Communications Engineering Journal 4, no. 3 (1992): 115. http://dx.doi.org/10.1049/ecej:19920021.

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9

Zhu, Zhou, Zhang Er-yang, and Lu Shu-jun. "A Kind of Cascade Anti-Jam Method for GPS Receiver." Procedia Engineering 23 (2011): 16–23. http://dx.doi.org/10.1016/j.proeng.2011.11.2458.

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10

Aulia, Cindy Rizka. "PENGARUH WAKTU DAN PELARUT EDTA (Ethylenediaminetetraacetic Acid) PADA EKSTRAKSI FUKOIDAN DARI RUMPUT LAUT COKELAT Sargassum binderi Sonder." Inovasi Pembangunan : Jurnal Kelitbangan 8, no. 03 (December 6, 2020): 265. http://dx.doi.org/10.35450/jip.v8i03.211.

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Rumput laut cokelat memiliki beragam polisakarida yang terkandung di dalamnya. Salah satu polisakarida dari rumput laut cokelat yang diketahui memiliki bioaktivitas yang tinggi adalah fukoidan. Banyak penelitian telah menunjukkan bahwa bioaktivitas dari fukoidan antara lain mampu menghambat pertumbuhan sel kanker, anti koagulan, anti virial, dan sebagai immunostimulan. Mengisolasi fukoidan dari rumput laut cokelat telah banyak dilakukan dengan beragam cara. Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh waktu dan konsentrasi pelarut EDTA (ethylenediaminetetraacetic acid) pada proses ekstraksi dan karakteristik fukoidan dari rumput laut cokelat jenis Sargassum binderi Sonder. Konsentrasi pelarut yang digunakan pada penelitian ini sebesar 0,15% ; 0,3%: 0,5%; 1%; dan 1,3% serta waktu ekstraksi selama 1 jam, 2 jam, 3 jam, 4 jam, dan 5 jam. Perbandingan rumput laut dan air adalah 1:30 dengan suhu ekstraksi dilakukan pada 70˚C. Dalam penelitian ini didapatkan yield crude fucoidan terbesar pada waktu ekstraksi 4 jam dan konsentrasi pelarut 1,3% yaitu sebesar 3,67%. Hasil analisis menunjukkan kandungan total gula dari ekstrak fukoidan dengan yield terbesar didapatkan sebesar 36,48% dan kandungan sulfat sebesar 17,16%.

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11

Sugano, Yasuyoshi, Masaki Takeuchi, Ayami Hirata, Hirokazu Matsushita, Toshio Kitamura, Minoru Tanaka, and Atsushi Miyajima. "Junctional Adhesion Molecule-A (JAM-A/JAM-1/F11R) Marks Long-Term Repopulating Hematopoietic Stem Cells." Blood 110, no. 11 (November 16, 2007): 1270. http://dx.doi.org/10.1182/blood.v110.11.1270.1270.

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Abstract Junctional adhesion molecule-A (JAM-A/JAM-1/F11R) is a cell adhesion molecule expressed in epithelial cells, endothelial cells and also hematopoietic cells such as leukocytes, platelets and erythrocytes. However, the expression of JAM-A in hematopoietic stem cell (HSC) had not been known. Here we show that JAM-A is expressed at a high level in the enriched HSC fraction, i.e. CD34+ c-Kit+ cells in embryonic day 11.5 (E11.5) aorta-gonod-mesonephros (AGM) and E11.5 fetal liver (FL) as well as c-Kit+ Sca-1+ Lineage- (KSL) cells in E14.5 FL, E18.5FL and adult bone marrow (BM). While the percentage of JAM-A+ cells in those tissues decreases during the development, the expression in HSC fraction is maintained throughout life. In fact, c-Kit+Sca-1+ cells are enriched approximately 200-fold from whole BM cells by anti-JAM-A antibody alone, i.e. the c-Kit+Sca-1+ cells in whole BM was about 0.15% and that in JAM-A+ cells was about 30%. Colony forming assays reveal that multi-lineage colony forming activity (CFU-mix) in JAM-A+ cells is higher than that in JAM-A- cells in the enriched HSC fraction in all those tissues. HSC transplantation assay revealed that long-term repopulating HSC (LTR-HSC) activity is present in the mice received 100 JAM-A+ KSL (7/9) cells and 300 KSL cells (5/6). Since the ratio of JAM-A+ cells to JAM-A- cells in the KSL fraction is about 6.5:3.5, 100 JAM-A- KSL cells are equivalent to 285 total KSL cells. In contrast to JAM-A+ cells, the mice received 1000 JAM-A- KSL cells, which are equivalent to more than 2850 total KSL cells, failed to engraft for long-term (0/6). These data revealed that long-term repopulating HSC (LTR-HSC) activity is present exclusively in the JAM-A+ cells, but not in JAM-A- cells. Moreover only 100 JAM-A+ cells isolated from whole BM cells by anti-JAM-A antibody alone reconstituted the hematopoietic system for long-term (4/7). Together these results indicate that JAM-A is expressed on hematopoietic precursors in various hematopoietic tissues and is an excellent and convenient marker to enrich LTR-HSC from BM cells.

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12

Makino, Akiko, Masayuki Shimojima, Takayuki Miyazawa, Kentaro Kato, Yukinobu Tohya, and Hiroomi Akashi. "Junctional Adhesion Molecule 1 Is a Functional Receptor for Feline Calicivirus." Journal of Virology 80, no. 9 (May 1, 2006): 4482–90. http://dx.doi.org/10.1128/jvi.80.9.4482-4490.2006.

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ABSTRACT The life cycle of calicivirus is not fully understood because most of the viruses cannot be propagated in tissue culture cells. We studied the mechanism of calicivirus entry into cells using feline calicivirus (FCV), a cultivable calicivirus. From the cDNA library of Crandell-Rees feline kidney (CRFK) cells, feline junctional adhesion molecule 1 (JAM-1), an immunoglobulin-like protein present in tight junctions, was identified as a cellular-binding molecule of the FCV F4 strain, a prototype strain in Japan. Feline JAM-1 expression in nonpermissive hamster lung cells led to binding and infection by F4 and all other strains tested. An anti-feline JAM-1 antibody reduced the binding of FCV to permissive CRFK cells and strongly suppressed the cytopathic effect (CPE) and FCV progeny production in infected cells. Some strains of FCV, such as F4 and F25, have the ability to replicate in Vero cells. We found that regardless of replication ability, FCV bound to Vero and 293T cells via simian and human JAM-1, respectively. In Vero cells, an anti-human JAM-1 antibody inhibited binding, CPE, and progeny production by F4 and F25. In addition, feline JAM-1 expression permitted FCV infection in 293T cells. Taken together, our results demonstrate that feline JAM-1 is a functional receptor for FCV, simian JAM-1 also functions as a receptor for some strains of FCV, and the interaction between FCV and JAM-1 molecules may be a determinant of viral tropism. This is the first report concerning a functional receptor for the viruses in the family Caliciviridae.

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13

Johnson-Léger, Caroline A., Michel Aurrand-Lions, Nicola Beltraminelli, Nicolas Fasel, and Beat A. Imhof. "Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration." Blood 100, no. 7 (October 1, 2002): 2479–86. http://dx.doi.org/10.1182/blood-2001-11-0098.

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The molecular mechanisms underlying lymphocyte extravasation remain poorly characterized. We have recently identified junctional adhesion molecule-2 (JAM-2), and have shown that antibodies to JAM-2 stain high endothelial venules (HEVs) within lymph nodes and Peyer patches of adult mice. Here we show that mouse lymphocytes migrate in greater numbers across monolayers of endothelioma cells transfected with JAM-2. The significance of these findings to an understanding of both normal and pathologic lymphocyte extravasation prompted us to clone the human homologue of JAM-2. We herein demonstrate that an anti–JAM-2 antibody, or a soluble JAM-2 molecule, blocks the transmigration of primary human peripheral blood leukocytes across human umbilical vein endothelial cells expressing endogenous JAM-2. Furthermore, we show that JAM-2 is expressed on HEVs in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration.

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14

Sani, Syeda Shaima, and Akbari Yaqub. "MIMO-OFDM Energy Efficient Cognitive System with Intelligent Anti-jam Capability." Wireless Personal Communications 98, no. 2 (November 9, 2017): 2291–317. http://dx.doi.org/10.1007/s11277-017-4975-8.

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15

Sugano, Yasuyoshi, Masaki Takeuchi, Ayami Hirata, Hirokazu Matsushita, Toshio Kitamura, Minoru Tanaka, and Atsushi Miyajima. "Junctional adhesion molecule-A, JAM-A, is a novel cell-surface marker for long-term repopulating hematopoietic stem cells." Blood 111, no. 3 (February 1, 2008): 1167–72. http://dx.doi.org/10.1182/blood-2007-03-081554.

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Abstract Junctional adhesion molecule-A (JAM-A/JAM-1/F11R) is a cell adhesion molecule expressed in epithelial and endothelial cells, and also hematopoietic cells, such as leukocytes, platelets, and erythrocytes. Here, we show that JAM-A is expressed at a high level in the enriched hematopoietic stem cell (HSC) fraction; that is, CD34+c-Kit+ cells in embryonic day 11.5 (E11.5) aorta-gonod-mesonephros (AGM) and E11.5 fetal liver (FL), as well as c-Kit+Sca-1+Lineage− (KSL) cells in E14.5 FL, E18.5FL, and adult bone marrow (BM). Although the percentage of JAM-A+ cells in those tissues decreases during development, the expression in the HSC fraction is maintained throughout life. Colony-forming assays reveal that multilineage colony-forming activity in JAM-A+ cells is higher than that in JAM-A− cells in the enriched HSC fraction in all of those tissues. Transplantation assays show that long-term reconstituting HSC (LTR-HSC) activity is exclusively in the JAM-A+ population and is highly enriched in the JAM-A+ cells sorted directly from whole BM cells by anti–JAM-A antibody alone. Together, these results indicate that JAM-A is expressed on hematopoietic precursors in various hematopoietic tissues and is an excellent marker to isolate LTR-HSCs.

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16

Khandoga, Andrej, Julia S. Kessler, Herbert Meissner, Marc Hanschen, Monica Corada, Toshiyuki Motoike, Georg Enders, Elisabetta Dejana, and Fritz Krombach. "Junctional adhesion molecule-A deficiency increases hepatic ischemia-reperfusion injury despite reduction of neutrophil transendothelial migration." Blood 106, no. 2 (July 15, 2005): 725–33. http://dx.doi.org/10.1182/blood-2004-11-4416.

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Abstract The endothelial receptors that control leukocyte transmigration in the postischemic liver are not identified. We investigated the role of junctional adhesion molecule-A (JAM-A), a receptor expressed in endothelial tight junctions, leukocytes, and platelets, for leukocyte transmigration during hepatic ischemia-reperfusion (I/R) in vivo. We show that JAM-A is up-regulated in hepatic venular endothelium during reperfusion. I/R-induced neutrophil transmigration was attenuated in both JAM-A-/- and endothelial JAM-A-/- mice as well as in mice treated with an anti-JAM-A antibody, whereas transmigration of T cells was JAM-A independent. Postischemic leukocyte rolling remained unaffected in JAM-A-/- and endothelial JAM-A-/- mice, whereas intravascular leukocyte adherence was increased. The extent of interactions of JAM-A-/- platelets with the postischemic endothelium was comparable with that of JAM-A+/+ platelets. The I/R-induced increase in the activity of alanine aminotransferase (ALT)/aspartate aminotransferase (AST) and sinusoidal perfusion failure was not reduced in JAM-A-/- mice, while the number of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL)-positive hepatocytes was significantly higher. Thus, we show for the first time that JAM-A is up-regulated in hepatic venules and serves as an endothelial receptor of neutrophil transmigration, but it does not mediate leukocyte rolling, adhesion, or platelet-endothelial cell interactions. JAM-A deficiency does not reduce I/R-induced microvascular and hepatocellular necrotic injury, but increases hepatocyte apoptosis, despite attenuation of neutrophil infiltration. (Blood. 2005;106:725-733)

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17

Itoh, Masahiko, Hiroyuki Sasaki, Mikio Furuse, Harunobu Ozaki, Toru Kita, and Shoichiro Tsukita. "Junctional adhesion molecule (JAM) binds to PAR-3." Journal of Cell Biology 154, no. 3 (August 6, 2001): 491–98. http://dx.doi.org/10.1083/jcb.200103047.

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At tight junctions (TJs), claudins with four transmembrane domains are incorporated into TJ strands. Junctional adhesion molecule (JAM), which belongs to the immunoglobulin superfamily, is also localized at TJs, but it remains unclear how JAM is integrated into TJs. Immunoreplica electron microscopy revealed that JAM showed an intimate spatial relationship with TJ strands in epithelial cells. In L fibroblasts expressing exogenous JAM, JAM was concentrated at cell–cell adhesion sites, where there were no strand-like structures, but rather characteristic membrane domains free of intramembranous particles were detected. These domains were specifically labeled with anti-JAM polyclonal antibody, suggesting that JAM forms planar aggregates through their lateral self-association. Immunofluorescence microscopy and in vitro binding assays revealed that ZO-1 directly binds to the COOH termini of claudins and JAM at its PDZ1 and PDZ3 domains, respectively. Furthermore, another PDZ-containing polarity-related protein, PAR-3, was directly bound to the COOH terminus of JAM, but not to that of claudins. These findings led to a molecular architectural model for TJs: small aggregates of JAM are tethered to claudin-based strands through ZO-1, and these JAM aggregates recruit PAR-3 to TJs. We also discuss the importance of this model from the perspective of the general molecular mechanisms behind the recruitment of PAR proteins to plasma membranes.

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18

Kwon, Taek-Sun, Jae-Gon Lee, and Jeong-Hae Lee. "Null Steering of Circular Array Using Array Factor for GPS Anti-Jam." Journal of Electromagnetic Engineering and Science 18, no. 4 (October 31, 2018): 267–69. http://dx.doi.org/10.26866/jees.2018.18.4.267.

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19

El Gamal, H., and E. Geraniotis. "Iterative channel estimation and decoding for convolutionally coded anti-jam FH signals." IEEE Transactions on Communications 50, no. 2 (2002): 321–31. http://dx.doi.org/10.1109/26.983327.

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20

Jerne, Christina. "The syntax of social movements: jam, boxes and other anti-mafia assemblages." Social Movement Studies 17, no. 3 (March 28, 2018): 282–98. http://dx.doi.org/10.1080/14742837.2018.1456327.

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21

Bradfield, Paul F., Christoph Scheiermann, Sussan Nourshargh, Christiane Ody, Francis W. Luscinskas, G. Ed Rainger, Gerard B. Nash, Marijana Miljkovic-Licina, Michel Aurrand-Lions, and Beat A. Imhof. "JAM-C regulates unidirectional monocyte transendothelial migration in inflammation." Blood 110, no. 7 (October 1, 2007): 2545–55. http://dx.doi.org/10.1182/blood-2007-03-078733.

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Monocyte recruitment from the vasculature involves sequential engagement of multiple receptors, culminating in transendothelial migration and extravasation. Junctional adhesion molecule-C (JAM-C) is localized at endothelial intercellular junctions and plays a role in monocyte transmigration. Here, we show that blockade of JAM-B/-C interaction reduced monocyte numbers in the extravascular compartment through increased reverse transmigration rather than by reduced transmigration. This was confirmed in vivo, showing that an anti–JAM-C antibody reduced the number of monocytes in inflammatory tissue and increased the number of monocytes with a reverse-transmigratory phenotype in the peripheral blood. All together, our results suggest a novel mechanism of reducing accumulation of monocytes at inflammation sites by disruption of JAM-C–mediated monocyte retention.

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Kalmykov, I. A., V. Sh Mukhametshin, K. T. Tyncherov, and M. V. Selivanova. "Developing method for constructing modular turbo code for anti-jam satellite authentication system." Journal of Physics: Conference Series 2176, no. 1 (June 1, 2022): 012023. http://dx.doi.org/10.1088/1742-6596/2176/1/012023.

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Abstract Low-earth orbit (LEO) satellite communication systems must have anti-jam property, which is based on information, structural and energy secrecy as well as immunity to jamming. One of the directions associated with increasing information secrecy is based on the use of a satellite identification system. This system is designed to prevent the imposition of foreign content on the subscriber through the use of an authentication protocol built on proof with zero knowledge. To reduce the time of applicant identification, a number of works propose to use modular codes (MC), which allow parallelizing the computational process in the protocol. It is known that MCs can improve the fault tolerance of the identification system since they are able to eliminate the consequences of faults and failures during operation. However, they can also be used to improve the immunity of LEO satellite communication systems to jamming. Thus, the use of a unified algebraic system when constructing MCs capable of correcting errors caused not only by faults and failures during the operation of the identification system but also by interference in the communication channel will enable to abandon concatenated codes. Therefore, the development of a method for constructing a modular turbo code for an anti-jam satellite authentication system is an urgent task.

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Fadhilah, Zulfa, Rani Prabandari, and Dwi Novitasari. "Formulasi Sediaan Masker Clay Ekstrak Etanol Kulit Buah Manggis (Garcinia mangostana L.) Sebagai Anti-Agging." Pharmacy Genius 1, no. 1 (October 20, 2022): 12–18. http://dx.doi.org/10.56359/pharmgen.v1i01.144.

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Pendahuluan: Masker tanah liat biasanya digunakan karena kemampuannya untuk meremajakan kulit. Saat masker mengering, mulai ada efek menarik pada tekstur kulit yang bisa anda rasakan perubahannya. Sensasi ini membantu menyegarkan kulit karena masker tanah liat menghilangkan kotoran dan komedo setelah wajah terkelupas, membuat kulit tampak cerah dan bersih setelah menggunakan masker. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui apakah ekstrak etanol kulit manggis dapat diformulasikan dalam masker lumpur yang stabil secara fisik, untuk mengetahui efek anti-penuaan ekstrak etanol kulit manggis, dan untuk mengetahui uji iritasi masker clay ekstrak etanol kulit buah manggis Metode: Metode yang digunakan adalah eksperimental dan alat ukur jangka sorong digital. Hasil: Sediaan masker clay ekstrak etanol kulit buah manggis mempunyai pH 5,7–6,4, homogen, dan stabil dalam penyimpanan suhu kamar, ekstrak etanol kulit buah manggis dengan konsentrasi 10% dan 12% lebih efektif menurunkan jumlah keriput dibandingkan konsentrasi 14% dan kontrol positif, dan uji iritasi terhadap kelinci yang dioleskan pada kulit punggungnya dan dibiarkan selama 4 jam lalu dilihat pada jam ke 1, 24, 48 dan 72 jam tidak menunjukan terjadinya iritasi Kesimpulan: Diperlukan penelitian lebih lanjut dalam bentuk sediaan lain menggunakan ekstrak kulit buah manggis sebagai anti aging

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Liu, Y., A. Nusrat, F. J. Schnell, T. A. Reaves, S. Walsh, M. Pochet, and C. A. Parkos. "Human junction adhesion molecule regulates tight junction resealing in epithelia." Journal of Cell Science 113, no. 13 (July 1, 2000): 2363–74. http://dx.doi.org/10.1242/jcs.113.13.2363.

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Epithelial cells form a highly selective barrier and line many organs. The epithelial barrier is maintained by closely apposed cell-cell contacts containing tight junctions, the regulation of which is incompletely understood. Here we report the cloning, tissue localization and evidence for a role in epithelial barrier regulation of an immunoglobulin superfamily member that likely represents the human homolog of murine junction adhesion molecule (JAM). Analysis of the primary structure of human JAM, cloned from T84 epithelial cells, predicts a transmembrane protein with an extracellular domain that contains two IgV loops. Monoclonal antibodies generated against the putative extracellular domain were reactive with a 35–39 kDa protein from both T84 epithelial cells and human neutrophils. By immunofluorescence, JAM mAbs labeled epithelial cells from intestine, lung, and kidney, prominently in the region of tight junctions (co-localization with occludin) and also along lateral cell membranes below the tight junctions. Flow cytometric studies confirmed predominant JAM expression in epithelial cells but also revealed expression on endothelial and hematopoietic cells of all lineages. Functional studies demonstrated that JAM specific mAbs markedly inhibited transepithelial resistance recovery of T84 monolayers after disruption of intercellular junctions (including tight junctions) by transient calcium depletion. Morphologic analysis revealed that, after disassembly of cell-cell junctions, anti-JAM inhibition of barrier function recovery correlated with a loss of both occludin and JAM, but not ZO-1, in reassembling tight junction structure. Reassembly of the major adherens junction component E-cadherin was not affected by JAM specific mAbs. Our findings suggest that JAM plays an important role in the regulation of tight junction assembly in epithelia. Furthermore, these JAM-mediated effects may occur by either direct, or indirect interactions with occludin.

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Wu, Zhi Yuan, Shu Hui Wang, Xin Li Tian, and Shu Zhang. "The Blocking Mechanisms and Chemical Modification for Alkane Grinding Fluid Applied for Si₃N₄ Ceramic Grinding." Advanced Materials Research 154-155 (October 2010): 569–72. http://dx.doi.org/10.4028/www.scientific.net/amr.154-155.569.

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A series of experiment were performed to test the cleaning ability of different alkane emulsion for Si3N4 ceramic grinding. The experiment results showed that the adsorption film surface of nonpolar alkane have strong attraction to Si3N4 abrasive dust, which will make abrasive dust to gather at the grinding area and finally led to jam. To add alkane grinding fluid with polar organic substances is beneficial to decrease jam. The best additive for anti block is which integrate hydrophobic group and hydrophilic group, both are indispensable.

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Aimi Azira, S., W. I. Wan Zunairah, M. Nor Afizah, Nor-Khaizura M.A.R., Radhiah S., Ismail Fitry M.R., and Nur Hanani Z.A. "Prevention of browning reaction in banana jam during storage by physical and chemical treatments." Food Research 5, no. 5 (September 10, 2021): 55–62. http://dx.doi.org/10.26656/fr.2017.5(5).046.

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Bananas are fruits that quickly turn brown after being peeled or cooked. The browning reaction reduces the quality of the appearance and shelf life of banana jam. Therefore, this study was aimed to evaluate the effect of chemical blanching and anti-browning agents on reducing browning reactions and maintaining the quality of banana jam during storage. In this study, Musa paradisiaca cv. Nipah was used to produce banana jam. The banana jam was prepared using three different treatments. The first treatment was prepared without hot water blanching treatment and with the addition of ascorbic acid and sodium metabisulphite. The jam was prepared with banana pulp, sugar, citric acid, and pectin. This treatment act as a control. The banana jam for the second and third treatments was prepared using the same ingredients as treatment one. In the second treatment, the sliced banana was blanched in hot water (80°C) for 10 mins, whereas in treatment three, the banana slices were dipped into 1.5% of ascorbic acid solution at 80°C for 10 mins. During the cooking process, 0.1% of sodium metabisulphite was added into the jam, for treatments two and three. The jam was cooked until the temperature reached 105°C and the total soluble solids range from 68 - 70°Bx. The banana jam was filled in glass jars, sterilized in a hot water bath at 80°C for 10 mins, cooled to 27°C (room temperature) before being stored at room temperature. The analysis observed were pH, total soluble solids, titrable acidity, colour, browning index, and textural properties. The samples were stored at room temperature for 60 days. The observations were made every 15 days for two months. After 60 days of storage, all treatments showed positive changes and a significant difference (p<0.05) in physicochemical and texture analyses. Overall, hot water blanching and chemical treatments significantly reduced the browning reaction in the banana jam. Therefore, treatment three had the best ability to slow down the browning reaction and deterioration rate of banana jam during room temperature storage.

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Naik, Meghna U., and Ulhas P. Naik. "Differential Regulation of bFGF- and VEGF-Induced Angiogenesis in Mice Lacking Junctional Adhesion Molecule-A." Blood 106, no. 11 (November 16, 2005): 533. http://dx.doi.org/10.1182/blood.v106.11.533.533.

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Abstract Angiogenesis, the formation of new blood vessels from preexisting vessels, is a complex process involving vascular endothelial cell activation, proliferation, migration, and tube formation. Several growth factors have been shown to induce this process. Among them vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are most widely studied. These two growth factors are believed to induce angiogenesis through distinct signaling pathways. Recently, we have shown that signaling through junctional adhesion molecule-A (JAM-A) is required for bFGF-induced angiogenesis. Here, we present evidence that JAM-A differentially regulates both bFGF-induced and VEGF-induced angiogenic responses. Using human umbilical cord vein endothelial cells (HUVEC) we found that bFGF-induced but not VEGF-induced migration was inhibited by function blocking anti-JAM-A antibody. We also found that mutant JAM-A blocked bFGF-induced but not VEGF-induced MAP kinase activation. To conclusively determine the role of JAM-A in angiogenic signaling, we generated JAM-A null mice. Endothelial cells isolated from JAM-A null mice failed to migrate in response to bFGF stimulation, but showed enhanced cell migration in response to VEGF. Further, using aortic ring and Matrigel® plug assays, we found that bFGF failed to induce blood vessel formation in JAM-A null mice. Interestingly, an increase in the number of blood vessels was observed in response to VEGF in these mice suggesting that in the absence of JAM-A bFGF-induced angiogenic pathway is impaired, but VEGF-induced pathway is upregulated to compensate. We next determined the effect of increased angiogenesis on tumor growth. We found that B16F0 melanoma growth was enhanced at least by two-fold in JAM-A null mice compared to wild-type mice. To understand the mechanism of hypersensitivity of JAM-A null mice to VEGF, we determined the expression of VEGF receptors on endothelial cells. We found that surface expression of VEGFR-2 is upregulated in endothelial cells isolated from JAM-A null mice. These results suggest that signaling through JAM-A is essential for the bFGF-induced pathway and when this pathway is impaired, the VEGF-induced pathway is upregulated which leads to enhanced angiogenesis and tumor growth.

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Bednarek, Radoslaw, Anna Selmi, Dagmara Wojkowska, Kamil Karolczak, Marcin Popielarski, Marta Stasiak, Moro O. Salifu, Anna Babinska, and Maria Swiatkowska. "Functional inhibition of F11 receptor (F11R/junctional adhesion molecule-A/JAM-A) activity by a F11R-derived peptide in breast cancer and its microenvironment." Breast Cancer Research and Treatment 179, no. 2 (October 24, 2019): 325–35. http://dx.doi.org/10.1007/s10549-019-05471-x.

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Abstract Purpose To examine the involvement of the F11R/JAM-A protein in breast cancer metastasis, we utilized the F11R/JAM-A antagonistic peptide 4D (P4D) in experiments of transendothelial migration (TEM) of breast cancer cells. Methods Experiments were conducted in the mouse 4T1 breast cancer model utilizing the human mammary epithelial cell and endothelial cell lines. The levels of soluble F11R/JAM-A (sJAM-A) in the murine plasmas were measured by ELISA. Levels of F11R/JAM-A mRNA and protein in cell lines were assessed by qRT-PCR and Western blot, respectively. Cell surface expression of F11R/JAM-A was demonstrated by flow cytometry. Functional tests included the TEM of breast cancer cells and adhesion of breast cancer cells to the endothelium. The endothelial permeability was studied by fluorescent tracer assay and by the Real-Time Cell Analysis (RTCA). Results The tumor inducers Tβ4 and TGF-β1 reduced the levels of sJAM-A in murine plasma, and reduced the F11R/JAM-A protein levels in the human microvascular endothelial cell line HMEC-1. The adhesion and TEM measured between breast cancer cells and inflamed or Tβ4-treated endothelium were inhibited by P4D. The presence of P4D did not destabilize the pre-existing tight junctions in the endothelial monolayer. The barrier-protecting effect of P4D was stronger than that of forskolin, when a booster dose of P4D was applied to the inflamed endothelium. Conclusions F11R/JAM-A protein can be considered as a novel target in the treatment of breast cancer metastasis. In vivo and clinical studies are needed to further investigate the effectiveness of F11R/JAM-A-derived peptide as a possible anti-metastatic drug.

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Chaudhary, Aditya, Khushbu Verma, and Baljeet Singh Saharan. "Probiotic Potential of Blueberry Jam Fermented with Lactic Acid Bacteria." Current Research in Nutrition and Food Science Journal 8, no. 1 (March 19, 2020): 65–78. http://dx.doi.org/10.12944/crnfsj.8.1.06.

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The present study assesses the feasibility of blueberry as a raw substrate for the production of the probiotic blueberry jam by lactic acid bacteria (L. plantarum DB-2, L. fermentum J-1, P. acidilactici M-3, L. plantarum SK-3, and P. pentosaceus SM-2). Changes in pH, titratable acidity (lactic acid), cell survival, antioxidant properties, and in vitro cholesterol reduction properties of lacto- fermented as well as non-fermented blueberry jam were examined during fermentation and up to 28 days of storage. All the strains grew well in a lacto-fermented blueberry jam after 48 h fermentation. Set A (5.88 g/100 ml) and Set B (5.96 g/100 ml) produced less lactic acid than Set C (6.67 g/100 ml) which has the consortia of probiotic strains. After 28 days of cold storage, all the tested strains survived the low-pH conditions in lacto-fermented blueberry jam. The blueberry jam fermented with the consortia of probiotic strains (Set C) had a high antioxidant capacity (71.47 ± 3.57) in comparison with Set A, Set B, and control which showed anti-oxidant capacity viz. 70.52 ± 3.52, 70.25 ± 3.18, and 64.12 ± 2.47, respectively after 28 days of refrigerated storage. The lacto- fermented blueberry jam in Set C (58.48%) had shown the in vitro cholesterol-lowering ability better than Set B (18.87%) whereas Set A and control did not show any in vitro reduction in cholesterol level after 28 days of storage. Sensory quality studies were carried out after 28 days of storage. Sensory evaluation data showed the considerable acceptability of the lacto-fermented blueberry jam. Finally, we found that L. plantarum DB-2, L. fermentum J-1, P. acidilactici M-3, L. plantarum SK-3, and P. pentosaceus SM-2 are optimal probiotics for fermentation with blueberry jam. In this investigation, the results could be an indicator of the development of health-promoting fruit jam. This lacto-fermented blueberry jam is a low-cost healthy food product, provide better nutrition and good health to the population.

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Yuda, Putu Era Sandhi Kusuma, I. Made Agus Mahardika, Erna Cahyaningsih, Maria Malida Vernandes Sasadara, Ni Made Dwi Mara Widyani Nayaka, and Ni Luh Kade Arman Anita Dewi. "Aktivitas Anti-Inflamasi Minyak Herbal Tradisional Dari Bahan Usada Bali Pada Mencit Inflamasi Yang Diinduksi Karagenan." JPSCR: Journal of Pharmaceutical Science and Clinical Research 7, no. 3 (November 30, 2022): 319. http://dx.doi.org/10.20961/jpscr.v7i3.60529.

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Peradangan sendi atau artritis merupakan salah satu penyakit inflamasi kronis yang memerlukan penggunaan obat jangka panjang. Penggunaan obat artritis seperti metotreksat dan NSAID dalam waktu lama dapat menimbulkan berbagai efek samping serius, sehingga diperlukan alternatif pengobatan yang relatif lebih aman terutama dari bahan herbal. Penelitian ini dilakukan untuk menguji aktivitas anti-inflamasi minyak herbal tradisional Usada Bali dari bahan Jahe (Zingiber officinale), Kunyit (Curcuma longa), Kencur (Kaemferia galanga), Bangle (Zingiber montanum), Cengkeh (Syzigium aromaticum) dan Kayu Manis (Cinnamomum burmanii) pada mencit inflamasi yang diinduksi karagenan melalui pengujian secara topikal. Mencit dibagi empat kelompok yang terdiri dari kelompok kontrol negatif (pembawa), kontrol positif (Natrium Diklofenak topikal), minyak herbal 150 dan 300 mg/ml. Volume peradangan kaki mencit diukur dengan alat pletismometer setiap jam selama empat jam setelah diinduksi dengan karagenan 0,5% (b/v) subplantar, kemudian diuji secara statistik (Mann-Whitney) dengan taraf kepercayaan 95%. Skrining fitokimia menunjukkan bahwa minyak herbal mengandung senyawa flavonoid, terpenoid dan steroid. Hasil uji aktivitas anti-inflamasi menunjukan adanya penghambatan peradangan yang signifikan (p<0,05) oleh minyak herbal konsentrasi 150 mg/ml maupun 300 mg/ml dengan persentase penghambatan pada jam ke-4 masing-masing sebesar 16,52% dan 11,30%, serta tidak berbeda bermakna dibandingkan kontrol positif (p>0,05). Hasil tersebut menunjukkan adanya potensi minyak herbal Usada Bali sebagai anti-inflamasi topikal.

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Budiarti, Eka, Perlambang Budiarti, Manggar Arum Aristri, and Irmanida Batubara. "Kolagen dari Limbah Tulang Ayam (Gallus gallus domesticus) terhadap Aktivitas Anti Aging secara In Vitro." ALCHEMY Jurnal Penelitian Kimia 15, no. 1 (March 14, 2019): 44. http://dx.doi.org/10.20961/alchemy.15.1.23046.44-56.

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Limbah tulang ayam meningkat seiring dengan peningkatan konsumsi ayam. Namun, belum banyak penelitian yang memanfaatkan limbah tulang sebagai sumber kolagen. Penelitian ini bertujuan mengetahui pengaruh ukuran kolagen terhadap aktivitas anti aging berupa aktivitas antioksidan, antiglikasi, dan inhibitor tirosinase secara in vitro dan mendapatkan teknik isolasi kolagen anti aging optimum dari tulang ayam. Isolasi kolagen dilakukan dengan variasi konsentrasi NaOH, yaitu 0,05 M; 0,10 M; dan 0,20 M, dilanjutkan dengan perendaman menggunakan asam asetat 1 M. Kolagen yang diisolasi dengan NaOH 0,10 M merupakan kolagen dengan ukuran partikel, rendemen, dan antiglikasi terbesar (berturut-turut 2,34 µm, 12,59%, 61,06%) dan memiliki spektrum inframerah yang paling sesuai dengan kolagen standar. Kolagen ini kemudian diaduk dengan kecepatan 1000 rpm selama 6 dan 8 jam untuk pengecilan ukuran. Kolagen dengan pengadukan 6 jam mempunyai ukuran partikel lebih kecil (1,34 µm) dibandingkan dengan pengadukan 8 jam (1,80 µm). Kolagen dengan ukuran 1,34 µm menunjukkan aktivitas terbaik yaitu aktivitas antioksidan terhadap 2,2-difenil-1-pikrilhidrazil (DPPH) sebesar 24,70% dan inhibitor tirosinase sebesar 26,77%. Berdasarkan aktivitas antioksidan, antiglikasi, dan antitirosinase, kolagen dengan perendaman NaOH 0,10 M dan pengadukan selama 6 jam memiliki sifat anti aging yang paling baik.In Vitro Anti-Aging Activity of Chicken (Gallus gallus domesticus) Bone Waste Collagen. Chicken bone waste increases with increasing chicken compsumtion. However, study on utilizing chicken bone for collagen source has not been widely explored. This study aims to determine the effect of collagen size on their anti aging activity, and to obtain the optimum condition to produce the chicken (Gallus gallus domesticus) collagen in the high yield and the best activity. Collagen isolation was carried out in various NaOH concentrations of 0.05 M, 0.10 M, and 0.20 M, followed by the maceration on acetic acid 1 M. The isolation in NaOH 0.10 M produced the collagen with particle size of 2.34 µm in yield of 12.59% and anti-glycation of 61.06%. The revealed infrared spectrum of the isolated collagen is almost the same with the spectrum of the standart collagen. The collagen in 2.34 µm was further stirred at a 1000 rpm for 6 and 8 hours to reduce the size. Collagen stirred in 6 hours has a smaller particle size (1.34 µm) compared with that of stirred in 8 hours which has a particle size of 1.80 µm. The collagen with size of 1.34 µm showed the best activity, which revealed the antioxidant activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) of 24.70% and tyrosinase inhibitors of 26.77%. Based on antioxidant activity, anti-glycation, and anti-tyrosinase, the collagen which was isolated in 0.10 M NaOH and was stirred in 6 hours has the best anti-aging property.

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Diabagate, Hadja M. F., Souleymane Traore, Doudjo Soro, Mohamed Cisse, and Kouakou Brou. "Biochemical Characterization and Nutritional Profile of Jam and Syrup from Saba senegalensis fruit in Côte d'Ivoire." Journal of Food Research 9, no. 6 (November 25, 2020): 67. http://dx.doi.org/10.5539/jfr.v9n6p67.

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Saba senegalensis is a plant to the family of Apocynaceae and its fruit called Saba is mainly used as food. For better valorisation, this study aimed to evaluate the nutritional potential of jam and syrup derived of this fruit. The study was carried out on the fruit of Saba senegalensis harvested in the north of Côte d'Ivoire. After jam and syrup formulation, pH, dry matter, ash, macronutrients, vitamins, minerals, phytonutrients, anti-nutritionals factors and nutritional profile have been determined. The results showed that jam and syrup of Saba were acidic with respective pH of 3.11 ± 0.01 and 3.65 ± 0.05. They contented higher in carbohydrates with respective rates of 56.53 ± 0.24 % and 66.27 ± 1.08 %. Vitamin C rate in jam and syrup was respectively about 20.01 ± 0.01 mg/100 g and 18.33 ± 2.22 mg/100 g. The most important mineral was potassium which rate is 136.71 ± 4.08 mg/100 g and 241.76 ± 5.9 mg/100 g in jam and syrup respectively. They also contain phytonutrients such as polyphenols (respectively 103.18 ± 0.69 mg/100 g and 3.29 ± 0.02 mg/100 g) and antinutritional factors such as oxalates (respectively 102.01 ± 6.93 mg/100 g and 19.96 ± 0.01 mg/100 g). Nutritional profile has classified Saba Senegalensis jam and syrup to the group 4 of foods, foods that must be eaten occasionally. The transformation of Saba in jam and syrup could be a good way to valorise this fruit and also ensuring its consumption through the year.

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Handayani, Sri Seno, Erin Ryantin Gunawan, and Dedy Suhendra. "Daya Repelan Lotion Minyak Ketapang pada Berbagai Variasi Konsentrasi Atsiri Kulit Jeruk Purut." Jurnal Pijar Mipa 16, no. 2 (March 3, 2021): 262. http://dx.doi.org/10.29303/jpm.v16i2.2220.

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Telah dilakukan penelitian lotion minyak ketapang dengan zat aktif tambahan minyak atsiri dari kulit jeruk purut sebagai anti nyamuk. Losion anti nyamuk yang tersedia di pasaran saat ini menggunakan bahan kimia sebagai bahan utama yang dapat menyebabkan resistensi terhadap nyamuk dan sangat berbahaya bagi kulit. Penelitian ini bertujuan untuk mengetahui uji tolak nyamuk losion minyak inti ketapang dengan penambahan variasi konsentrasi minyak atsiri kulit jeruk purut. Semua uji sifat fisik memenuhi kriteria sediaan lotion. pH dan daya sebar lotion diuji lagi setelah disimpan selama satu bulan. Berdasarkan hasil evaluasi diperoleh bahwa formula I, II dan III stabil secara fisik selama satu bulan penyimpanan dengan pH berkisar antara 6,7-7,0. pH lotion tidak mengalami perbedaan bermakna dibandingkan dengan ketika baru dibuat, sedangkan daya sebarnya mengalami perubahan setelah disimpan satu bulan. Daya repelan tertinggi dimiliki oleh lotion dengan konsentrasi minyak atsiri 3%. Daya repelan lotion dapat bertahan selama 3 jam, setelah lebih dari 3 jam, efektifitas daya repelan lotion berkurang.

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Suharyanisa, Suharyanisa, Jon Kenedy Marpaung, Ivan Elisabeth Purba, and Bengi Simahara. "PENGUJIAN EFEK DIURETIK INFUSA DAUN KOPI (Coffea arabica L.) PADA TIKUS PUTIH JANTAN GALUR WISTAR." MEDFARM: Jurnal Farmasi dan Kesehatan 11, no. 2 (December 28, 2022): 161–74. http://dx.doi.org/10.48191/medfarm.v11i2.139.

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Diuretik bekerja pada ginjal dengan cara meningkatkan eksresi air dan natrium klorida. Kecenderungan masyarakat mengonsumsi diuretik berbahan kimia menyebabkan banyaknya efek negatif yang ditimbulkan. Penggunaan bahan alam sudah banyak diteliti untuk menggantikan obat-obatan kimia. Salah satu bahan alam yang memiliki khasiat yaitu tanaman kopi. Tanaman kopi yang digunakan bagian daunnya mengandung senyawa-senyawa antioksidan (flavonoid, alkaloid, saponin, kafein dan polifenol) yang bermanfaat sebagai anti inflamasi (anti peradangan), anti kanker, diuretik, antimikroba dan aktivitas antioksidan. Infusa Daun Kopi (IDK) diperoleh menggunakan teknik infusa dengan pelarut akuades kemudian digunakan pada 25 ekor tikus yang dibagi menjadi 5 kelompok. Kelompok I (negatif) Na-CMC 0,5 %, kelompok II (positif) Furosemid 3,6 mg/kgBB, kelompok III, IV, V IDK dosis 10, 20, 40% diberikan secara oral. Tikus diberi akuades secara oral dengan dosis 15 ml/kgBB. Tikus di letakkan di dalam kandang metabolik, kemudian pemeriksaan fisik urin dilakukan setiap 1 jam sekali selama 6 jam. Hasil penelitian menunjukkan bahwa IDK dengan dosis terbaik adalah 40% yang memberikan peningkatan volume urin, memberi pengaruh terhadap pH urin, memberi pengaruh terhadap berat jenis urin, memberi pengaruh terhadap warna urin dan memberi pengaruh terhadap kejernihan urin.

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Mukhopadhyay, Mainak, Binay Kumar Sarkar, and Ajay Chakraborty. "AUGMENTATION OF ANTI-JAM GPS SYSTEM USING SMART ANTENNA WITH A SIMPLE DOA ESTIMATION ALGORITHM." Progress In Electromagnetics Research 67 (2007): 231–49. http://dx.doi.org/10.2528/pier06090504.

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Thyagarajan, V., and S. Kaja Mohideen. "GPS Jamming: Strengthening Anti Jam GPS System with Adaptive Phase Only Nulling Using Cuckoo Search." Research Journal of Applied Sciences, Engineering and Technology 8, no. 5 (August 5, 2014): 679–86. http://dx.doi.org/10.19026/rjaset.8.1022.

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Yanjun, Zhang, and Li Ping. "Anti-jam Adaptive Array Instrument for the Weak Gnss in the Fast Time-varying Scenario." Information Technology Journal 12, no. 24 (December 1, 2013): 8446–53. http://dx.doi.org/10.3923/itj.2013.8446.8453.

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Seo, Jiwon, Yu-Hsuan Chen, David S. De Lorenzo, Sherman Lo, Per Enge, Dennis Akos, and Jiyun Lee. "A Real-Time Capable Software-Defined Receiver Using GPU for Adaptive Anti-Jam GPS Sensors." Sensors 11, no. 9 (September 19, 2011): 8966–91. http://dx.doi.org/10.3390/s110908966.

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Progri, Ilir F. "Advanced Anti-Jam Indoor Adaptive GNSS Signal Acquisition: Part 1, Normal Distribution--Theory and Simulations." Journal of Geolocation, Geo-information and Geo-intelligence 2018, no. 1 (2018): 71. http://dx.doi.org/10.18610/jg3.2018.071604.

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Doñate, Carmen, Archana Vijaya Kumar, Beat A. Imhof, and Thomas Matthes. "Anti-JAM-C therapy eliminates tumor engraftment in a xenograft model of mantle cell lymphoma." Journal of Leukocyte Biology 100, no. 5 (June 2, 2016): 843–53. http://dx.doi.org/10.1189/jlb.1hi1114-549rr.

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Moroz, Alexander Petrovich, Igor Mikhailovich Beluchenko, Kim Leonidovich Samarov, and Yuri Veniaminovich Strenakuk. "Singularities of the Data Anti-jam in Telecommunication Systems Using the New Methods of Encryption." Biosciences Biotechnology Research Asia 11, Spl Edition Nov. 14 (April 30, 2014): 133–37. http://dx.doi.org/10.13005/bbra/1450.

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Naik, Meghna Ulhas, Vesselina Cooke, Sharmila Chatterjee, and Ulhas P. Naik. "Angiogenesis Is Suppressed by Junctional Adhesion Molecule A Through Transcription Factors HIF-1a- and Id1-Dependent Modulation of VEGF/VEGFR2 Expression." Blood 120, no. 21 (November 16, 2012): 618. http://dx.doi.org/10.1182/blood.v120.21.618.618.

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Abstract Abstract 618 The process of angiogenesis is associated with a number of human pathologies. Vascular endothelial growth factor (VEGF) is a potent angiogenic growth factor. VEGF expression is upregulated during ischemia and tumor growth. VEGF is also expressed in healthy adult vascular tissue. However, VEGF-dependent angiogenesis is normally suppressed in healthy adults. The mechanism of this suppression is not known. Here we show that junctional adhesion molecule A (JAM-A), a tight junction protein, is an endogenous suppressor of VEGF-induced angiogenesis. Using gene-targeted Jam-A null mice, we found that murine melanoma tumor growth and associated angiogenesis were significantly augmented (P<0.001) in Jam-A null mice compared to wild-type mice. Additionally, Jam-A null mice showed significantly enhanced (P<0.004) vascular permeability as assessed by a Miles assay using Evans blue dye. Furthermore, VEGF-, but not FGF-2-dependent angiogenesis is significantly augmented (P<0.001) in the absence of Jam-A as assessed by Matrigel plug and aortic ring sprouting, two well-accepted in vivo and ex vivo angiogenic assays, respectively. Vascular endothelial cells isolated from Jam-A null mouse aorta showed significantly enhanced (P<0.05) cell migration and tube-like structure formation (P<0.05) in response to VEGF. Additionally, we found the plasma levels of VEGF in Jam-A null mice is significantly (P<0.000001) and age-dependently increased compared to WT mice. Furthermore, both mRNA and protein levels of VEGF and its receptor VEGFR2 were significantly increased (P<0.001) in Jam-A null endothelial cells, suggesting that the VEGF/VEGFR2 signaling axis is enhanced in the absence of Jam-A. To further confirm this finding, we injected anti-VEGFR2 (DC101) into the Jam-A null mice inoculated with murine melanoma (B16F0) cells. Inhibition of VEGF/VEGFR-2 signaling by DC101 significantly reduced (P<0.00003) tumor growth and associated angiogenesis in Jam-A null mice. Additionally, vascular permeability observed in Jam-A null mice was completely abrogated upon DC101 treatment. When tested if the expression of soluble Flt (sFlt), which is known to trap VEGF, is downregulated in the absence of Jam-A, we found no significant difference in sFlt mRNA expression in Jam-A null endothelial cells compared to WT. In order to determine the mechanism of this upregulation of the VEGF signaling axis, we tested the expression of hypoxia inducible factor 1a (HIF1-a) and inhibitor of DNA binding 1 (Id1), two transcription factors known to upregulate VEGF and VEGFR2 gene expression. Interestingly, mRNA and protein levels of both HIF1-a and Id1 were significantly augmented (P<0.02) in endothelial cells lacking Jam-A. Consistent with this finding, the overexpression of JAM-A in HUVECs attenuated the levels of Id1. These results suggest that JAM-A suppresses VEGF/VEGFR2 expression in endothelial cells by attenuating HIF1-a and Id1 expression, thus suppressing adult angiogenesis. During pathological conditions such as ischemia and tumor growth, it is possible that JAM-A levels are downregulated, thus supporting pathological angiogenesis. Disclosures: No relevant conflicts of interest to declare.

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Tatura, Suryadi Nicolaas Napoleon. "Efikasi Obat Kloroquine, Kina, Artesunate-SP, Artesunate-Amodiaquine, Artesunate-Lumafentrin pada Anak Malaria Falciparum di BLU RSUP Prof. Dr. RD. Kandou Manado." Sari Pediatri 10, no. 6 (November 29, 2016): 417. http://dx.doi.org/10.14238/sp10.6.2009.417-23.

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Latar belakang. Malaria falciparum masih merupakan salah satu penyebab utama kematian dan kesakitan pada anak-anak dan orang dewasa di negara-negara tropis. Di Indonesia, dilaporkan Plasmodium falciparum telsh resisten terhadap obat – obat anti malaria, terutama kemungkinan terjadi early treatment failure (ETF).Tujuan. Untuk mengetahui efikasi dan early treatment failure (ETF) obat anti malaria (OAM) yaitu kloroquin (CQ), kina, artesunat-SP(AS-SP), dan artesunate-amodiaquin(AS-AQ) serta artesunate- lumafentrin(AS-L) pada anak dengan malaria falciparum.Metode. Penelitian deskriptif retrospektif� �� . Populasi adalah bayi dan anak usia 1 bulan-14 tahun yang terdiagnosis dengan malaria falciparum dan mendapat OAM. Data diperoleh dari rekam medis Bagian Anak BLU RSUP Prof Dr. RD. Kandou Manado sejak Maret 2007 sampai Maret 2009. Data dikelompokkan berdasarkan jenis obat anti malaria yang digunakan oleh pasien selama 3 hari (3x24jam), kemudian dinilai waktu bebas parasit dalam darah pasien serta ETF. Hitung parasit menggunakan metode semikuantitatif. Data dianalis dengan metode Kaplan-Meier menggunakan SPSS 17.Hasil. Efikasi obat anti malaria dalam 3 hari sebagai berikut, AS-L 100%, AS-SP 100%, AS-AQ 97%, CQ 85%, dan kina 81%. Terdapat ETF obat kina 19%, CQ 15% dan AS-AQ 3%. Parasite negative rate dalam 24 jam AS-SP 0,6, AS-L 0,6, AS-AQ 0,89, Kina 0,35 dan CQ 0,54.Kesimpulan. Artesunate-lumafentrin dan artesunate-SP merupakan obat anti malaria falciparum pilihan. Artesunate-amodiaquine sangat baik menurunkan angka parasit dalam 24 jam I. Telah terjadi ETF pada kloroquine, kina dan arteunate-amodiaquine.

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Jamiludin, Jamiludin, and Husain AAA. "Efektivitas Midazolam Untuk Pencegahan Mual Muntah Pascabedah Pada Prosedur Laparaskopi." JAI (Jurnal Anestesiologi Indonesia) 5, no. 3 (November 1, 2013): 172. http://dx.doi.org/10.14710/jai.v5i3.6307.

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Latar belakang : Seluruh pasien yang menjalani pembedahan beresiko untuk mengalami mual dan muntah pasca bedah (PONV). Kejadian PONV menjadi gejala yang sangat merugikan terutama setelah prosedur pembedahan ambulatori serta mengganggu proses pemulihan pasca anestesi dan pembedahan sehingga memperpanjang waktu perawatan. Laparoskopi adalah suatu prosedur pembedahan minimal invasif yang disertai insidens PONV cukup tinggi. Penyebab tingginya angka kejadian PONV pada pembedahan laparaskopi disebabkan oleh gas yang digunakan untuk insuflasi dan menyebabkan penekanan pada nervus vagus yang memiliki hubungan dengan pusat muntah di medulla oblongata. Selain itu, penyebab lain seperti teknik anestesi, jenis kelamin, nyeri, perawatan pasca operatif dan data demografik pasien yang berhubungan dengan pengaruh terjadinya emesis. Midazolam sebagai agen anti emetik dan anxiolitik yang menurunkan sintesis, pelepasan dan efek pasca sinaptik dopamin serta menhambat reuptake adenosin, sehingga menurunkan input dopamin dan 5-HT3 terhadap CRTZ dan mengurangi input dari thalamus yang mempengaruhi langsung pusat muntah.Tujuan: mengevaluasi pemberian midazolam sebagai agen anti emetik dan anxiolitikMetode: Empat puluh delapan pasien yang akan menjalani prosedur pembedahan laparaskopi elektif secara acak dibagi menjadi dua kelompok. Setelah diberikan obat premedikasi, kelompok M (n=24) diberikan midazolam 35 μg/kgBB intravena kelompok O (n=24) diberikan ondansetron 4 mg intravena. Selama prosedur anestesi, pemakaian opioid dan lama operasi dicatat. Kemudian kejadian mual muntah pasca bedah diamati dan dicatat selama periode 8 jam pascabedah.Hasil: Kejadian mual muntah setelah prosedur pembedahan laparaskopi pada penelitian ini diukur menggunakan skor PONV dengan interval 30 menit selama di ruang pemulihan dan setiap 1 jam di ruang perawatan selama 8 jam pasca bedah. Pada penilitian ini, terdapat perbedaan yang bermakna diantara kedua kelompok dengan hasil p=0,022 (p<0,05) pada waktu pengamatan P2 (60 menit pasca bedah) .Kesimpulan: Midazolam 35 μg/kgBB setelah premedikasi pada anestesi umum pada prosedur pembedahan laparaskopi elektif menurunkan kejadian mual muntah pascabedah terutama pada 1 jam pasca bedah.

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Satiyah, Umi, Djudjuk Rahmad Basuki, and Ristiawan Muji Laksono. "Pengaruh Pemberian Pre Emptive Ketamin 0,15 mg/kgbb iv Terhadap Intensitas Nyeri Pasca Operasi Bedah Onkologi Mayor Dengan Anestesi Umum Di RSUD Dr Saiful Anwar Malang." JAI (Jurnal Anestesiologi Indonesia) 7, no. 3 (November 1, 2015): 197. http://dx.doi.org/10.14710/jai.v7i3.10811.

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Latar Belakang : Preemptive analgesia dan multimodal analgesia mempunyai peranan penting dalam penanganan nyeri selama dan pasca operasi dengan memblok jalur nyeri yang terdiri dari tranduksi, transmisi, modulasi dan persepsi. Ketamin sebagai Anti NMDA (N metil D Aspartat) reseptor bekerja memblok jalur transmisi dan modulasi serta sinergis dengan opioid. Ketamin dosis kecil 0,15 mg/kgbb mempunyai efek preemptive analgesia dan tidak memiliki efek samping yang berat.Tujuan : mengetahui pengaruh pemberian preemptive ketamin 0,15 mg/kgbb iv terhadap intensitas nyeri pasca bedah onkologi mayor dengan anestesi umum pada 1,2 dan 3 jam pasca operasiMetode : Penelitian ini merupakan uji klinis acak tersamar ganda, bersifat eksperimental. Sampel penelitian adalah pasien usia 17-40 tahun, kriteria klinis ASA I-II, pendidikan minimal SMP, dan BMI antara 20-30 kg/M2 yang menjalani pembedahan elektif bedah onkologi mayor kategori nyeri sedang yang meliputi operasi struma dan mammae selain radikal mastektomi (MRM). Jumlah sampel adalah 44 pasien yang dibagi secara random menjadi 2 kelompok, yaitu kelompok 1 (perlakuan) yang menerima ketamin 0,15 mg/kgbb dan kelompok 2 (kontrol) tanpa menerima ketamin 0,15mg/kgbb. Semua pasien menerima (multimodal analgesia) yaitu fentanyl (opioid), ketorolac (NSID) dan juga obat2an lain untuk anestesi umum. Intensitas nyeri pada semua sampel diamati pada 1, 2 dan 3 jam pasca operasi dengan menggunakan Verbal numerical rating scale (VNRS) yang setara dengan VAS (visual Analogue scale). Uji statistik normalitas menggunakan uji saphiro wilk diperoleh hasil bahwa data yang ada tidak terdistribusi normal sehingga dilakukan uji beda non parametrik mann whitney testHasil : Penelitian ini menunjukkan bahwa pemberian ketamin 0,15 mg/kgbb mengurangi nyeri akut lebih baik pada 1, 2 dan 3 jam pasca operasi. Pada 1 jam pasca operasi kelompok perlakuan memiliki nilai rerata VAS 0 atau lebih rendah 0,77 cm dbandingkan kelompok kontrol dengan nilai p<0,001. Pada 2 jam pasca operasi kelompok perlakuan memiliki rerata VAS 0,3 cm atau lebih rendah 1,4 cm bila dibandingkan dengan kelompok kontrol dengan nilai p<0,001. Pada 3 jam pasca operasi kelompok perlakuan memiliki rerata 0,9 cm atau lebih rendah 1,6 cm dengan nilai p<0,001.Kesimpulan : Pada penelitian ini preepmtive ketamin 0,15 mg/kgbb iv memberikan pengaruh menurunkan intensitas nyeri pada 1 jam, 2 jam dan 3 jam pasca pembedahan onkologi mayor kategori nyeri sedang.

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Pringgenies, Delianis, Rini Widiyadmi, Ervia Yudiati, Muhammad Syaifudien Bahry, and Ali Djunaedi. "Potensi Ekstrak Buah Mangrove Xylocarpus granatum Untuk Pemberantasan Larva Nyamuk Aedes aegypti." Journal of Tropical Marine Science 1, no. 1 (December 5, 2018): 1–6. http://dx.doi.org/10.33019/jour.trop.mar.sci.v1i1.657.

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ABSTRAK Tujuan penelitian adalah untuk mengetahui potensi ekstrak buah mangrove Xylocarpus granatum sebagai anti larva nyamuk Aedes aegypti. Uji larva nyamuk dengan memasukkan masing-masing 10 ekor larva nyamuk yang dimasukkan ke dalam 7 botol cup tranparan kecil, masing– masing untuk 50, 100, 250, 500, 1000 ppm sampel ektrak, kontrol negative dan kontrol positive. Hasil penelitian memperlihatkan bahwa pengaruh tingkat mortalitas larva nyamuk terhadap ekstrak buah mangrove Xylocarpus granatum pada jam 1 memperlihatkan bahwa pada konsentrasi 1000 ppm, persentasi mortalitas nyamuk tertinggi adalah 81,34 %, dan pada konstrasi ekstrak buah mangrove Xylocarpus granatum 100 ppm terendah mortalitasnya = 68,8%. Perlakuan pada jam 3 memperlihatkan bahwa pada konsentrasi ekstrak buah mangrove 500 ppm tingkat mortalitas larva nyamuk tertinggi (80%). Pada konsentrasi ekstrasi buah mangrove 250 ppm, mortalitasnya 66,70% dan konsentrasi ekstral buah mangrove pada 100 ppm, mortalitasnya adalah 40%. Hasil peneltian pada jam ke 24 memperlihatkan bahwa pada konsentrasi ekstrak buah mangrove mencapai 1000 pmm, mortalitasnya 100%, pengamatan jam ke 48 mortalitasnya 100%. Sedang pada perlakuan control positif, keluulushidupan larva nyamuk adalah 0% dan pada control negative, kelulushidupan larva nyamuk adalah 100%. Kesimpulan: bahwa ekstrak buah mangrove Xylocarpus granatum berpotensi sebagai anti larva nyamuk pada konsentrasi 1000 ppm. ABSTRACT It was assumed that mangrove fruits has some insecticidal biosubstances. Aim of the research is the potentials of mangrove Xylocarpus granatum fruit extract as the anti mosquito (Aedes aegypti) repellent. Experiment using of 10 mosquito larvae in 7 bottles each filled with 50; 100; 250; 500 and 1000 ppm fruit extract, negative and positive control. The experiment reveals that after 1 hour treatment, the 1000 ppm exctract had the hihgest mosquito larvae mortality of 81.34%, while the lowest extract of 100 ppm had 68.8% mortality. After 3 hours of extract treatment the 500 ppm had the hihgest mortality of 80%, 250 ppm with 66.70% and 100 ppm with 40 % mortality. Result of experiment after 24 as well as 48 hours treatment the 1000 ppm extract had 100% mortality. In the positive control had 0% mortality and the negative control had 100% of survival. The summary is that the mangrove Xylocarpus granatum fruit extract had a potential as mosquito repellent at 1000 ppm fruit extract.

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Terral, Guillaume, Thierry Champion, François Debaene, Olivier Colas, Maxime Bourguet, Elsa Wagner-Rousset, Nathalie Corvaia, Alain Beck, and Sarah Cianferani. "Epitope characterization of anti-JAM-A antibodies using orthogonal mass spectrometry and surface plasmon resonance approaches." mAbs 9, no. 8 (October 16, 2017): 1317–26. http://dx.doi.org/10.1080/19420862.2017.1380762.

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Asadullah, M. M. G., and G. L. Stuber. "Joint iterative channel estimation and soft-chip combining for a MIMO MC-CDMA anti-jam system." IEEE Transactions on Communications 57, no. 4 (April 2009): 1068–78. http://dx.doi.org/10.1109/tcomm.2009.04.070013.

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Azari, Bani M., Marc J. Braunstein, H. Uwe Kluppelberg, Sadeaqua S. Scott, Eric LP Smith, Jonathan D. Marmur, Anna Babinska, and Olcay Batuman. "Junctional Adhesion Molecule-A/ F11 Receptor (JAM-A/ F11R) Expression in Multiple Myeloma (MM): a Candidate Biomarker of Aggressive Disease." Blood 114, no. 22 (November 20, 2009): 2830. http://dx.doi.org/10.1182/blood.v114.22.2830.2830.

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Abstract Abstract 2830 Poster Board II-806 Background: Multiple myeloma (MM) is an incurable disease of clonal plasma cells that accumulate in the bone marrow (BM), causing monoclonal IG production, bone marrow failure, osteolytic lesions and kidney disease. Although initially treatable, MM ultimately becomes refractory to treatment, and is invariably fatal, when tumor cells that harbor genetic mutations expand without regulation. Therefore novel treatment targets need to be identified. A key mechanism in MM pathogenesis is regulation of tumor growth by the bone marrow (BM) microenvironment, particularly by bone marrow neo-vascularization and adhesion of tumor cells to the marrow stroma. Aberrantly expressed genes that regulate angiogenesis by MM cells enhance MM progression and constitute targets in its treatment. JAM-A/F11R is an endothelial cell (EC) adhesion molecule of the immunoglobulin superfamily which is a multifunctional cell membrane protein that mediates intracellular signaling events that alter EC migration and paracellular permeability. For example, in breast cancer, attenuation of JAM-A increases tumor invasion and metastasis through a decrease in tumor adhesion (Ulas Naik Cell Adh Migr. 2008 Oct;2(4):249-51.). In this study we explored the JAM-A/F11R expression in MM tumor cells and in patients to determine the potential role of this molecule in the pathogenesis and progression of MM. Methods: The MM cell lines examined were RPMI-8266, U266, and NCI-H929. Human umbilical vein endothelial cells (HUVECs) served as controls. Informed consent was obtained from patients and control subjects. Primary BM tumor cells were enriched to > 95% CD138+ cells by positive selection using anti-CD138 MACS MicroBeads. The CD138-negative fraction was used for outgrowth of confluent EPCs (> 98% vWF/CD133/KDR+). JAM-A mRNA expression was assessed using an microarray gene expression profile, JAM-A probe based real-time PCR, and JAM-A levels in each sample were measure using a standard curve and normalized to GADPH. JAM-A protein levels in MM cell lines and primary tumor cells were measured by flow cytometry and immunofluorescence. For serum studies, peripheral blood was obtained from 25 newly diagnosed MM patients and 8 healthy, age- and sex-matched controls, and JAM-A levels were measured using an ELISA. Statistical analysis was performed using Student's t-test, two-tailed, with P ' .05 considered significant. Results: JAM-A mRNA levels were significantly increased in MM cell lines RPMI-8266, U266, and NCI-H929 compared to HUVECs (U266, P = 3×10-5; RPM1-8266, P = 1×10-6; NC1-H929, P= 5×10-4). The JAM-A mRNA levels were significantly greater in RPMI-8226; P < .04 compared to TNFα-activated HUVECs for 24 hours which is a proangiogenic switch for HUVEC gene expression. The elevated mRNA expression of the JAM-A in MM cell lines was confirmed by immunofluorescence and flow cytometry which showed the presence of both membrane and cytoplasmic JAM-A protein. Microarray analysis of gene expression profiles from 20 patients' corresponding tumor cells and microenvironmental EPCs showed that JAM-A had a higher level of expression in tumor cells versus MM EPC by 12.62 fold, (P=.0000642). Furthermore, JAM-A had a higher level of expression in MM EPC versus normal control EPC by 2.41 fold, (P=.00113) reflecting a complex regulatory role of F11 signaling in MM, similar to breast cancer (Naik, U. et al 2008). JAM-A was also found to be 12.6 fold greater in tumor cells compared to EPCS (P=.0000642). In addition, circulating levels of soluble JAM-A were found to be significantly greater in the serum of MM patients compared to controls (P < .005), with an average 2-fold increase. Serum levels of JAM-A in MM patients also decreased 71% with treatment n=5, P<.05. Conclusion: We show for the first time that JAM-A expression is highly elevated in MM tumor cells and its levels respond to treatment. In addition, MM patients have higher circulating JAM-A levels compared to healthy individuals and circulating JAM-A levels were reduced following treatment, suggesting that JAM-A may serve as a novel biomarker in MM. Current studies in the lab are aimed at correlating these levels with clinical parameters to determine whether JAM-A levels reflect disease severity and response to treatment. Results of these analyses, as well as results of ongoing experiments using JAM-A siRNA and antibody-inhibition approaches to target JAM-A in myeloma tumor and ECs will be presented. Disclosures: No relevant conflicts of interest to declare.

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Andriyani, Wiwien Mukti, Sri Murtini, Alimuddin Alimuddin, and I. W. Teguh Wibawan. "EKSPRESI PROTEIN COAT DAN mRNA VIRAL NERVOUS NECROSIS YANG DIKENDALIKAN OLEH PROMOTER β-AKTIN IKAN MEDAKA DAN KERATIN IKAN FLOUNDER JEPANG." Jurnal Riset Akuakultur 9, no. 1 (March 31, 2014): 79. http://dx.doi.org/10.15578/jra.9.1.2014.79-86.

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Kemampuan promoter dalam mengatur ekspresi gen penyandi protein imunogenik sangat menentukan efikasi suatu vaksin DNA. Penelitian ini bertujuan untuk mengukur tingkat ekspresi protein dan mRNA RNA2 penyandi coat protein (CP) virus viral nervous necrosis (VNN) yang dikendalikan oleh dua promoter berbeda, yaitu promoter β-aktin ikan medaka (mBA), dan keratin ikan flounder Jepang (JfKer). Uji ekspresi CP dilakukan menggunakan embrio ikan lele dumbo (Clarias sp.) sebagai model, sedangkan analisis mRNA dilakukan menggunakan ikan kerapu tikus. Konstruksi vektor ekspresi pmBA-CP dan pJKer-CP dengan konsentrasi 50 ng/μL KCl 1 M disuntikkan ke embrio ikan lele dumbo fase 1-2 sel. Sebanyak 30 embrio ikan lele dumbo diambil pada jam ke-6, 8, 10, 12, 14, dan 16 pascainjeksi untuk analisis protein. Hasil SDS-PAGE menunjukkan adanya protein berukuran sekitar 42 kDa, dan analisis western blot menggunakan antibodi (Ab) poliklonal anti-VNN membuktikan bahwa protein tersebut adalah CP. Keberhasilan deteksi protein spesifik menggunakan Ab anti-VNN tersebut menunjukkan bahwa embrio ikan lele dapat digunakan untuk menguji potensi produksi protein imunogenik yang dikendalikan oleh promoter berbeda. Pengujian ini juga menunjukkan bahwa, aktivitas promoter mBA lebih tinggi daripada promoter JfKer, sehingga uji ekspresi mRNA dilakukan menggunakan konstruksi pmBA-CP. Benih ikan kerapu tikus (panjang badan sekitar 5 cm) diinjeksi dengan pmBA-CP secara intramuskular dengan dosis 12,5 μg/ekor. Total RNA diekstraksi dari daging pada waktu 6, 12, dan 24 jam pascainjeksi. Hasil RT-PCR menunjukkan adanya ekspresi mRNA CP pada 24 jam pascainjeksi. Hal tersebut menunjukkan bahwa promotor mBA aktif mengendalikan ekspresi CP pada ikan kerapu tikus, dan pmBA-CP berpotensi digunakan sebagai vaksin DNA untuk menginduksi kekebalan ikan kerapu terhadap infeksi VNN.

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Journal articles on the topic 'Anti-jam'

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