Academic literature on the topic 'Animal repellants'

Abstract Naphthalene, a volatile organic compound present in moth repellants and petroleum-based fuels, has been shown to induce toxicity in mice and rats during chronic inhalation exposures. Although simpler default methods exist for extrapolating toxicity points of departure from animals to humans, using a physiologically based pharmacokinetic (PBPK) model to perform such extrapolations is generally preferred. Confidence in PBPK models increases when they have been validated using both animal and human in vivo pharmacokinetic (PK) data. A published inhalation PBPK model for naphthalene was previously shown to predict rodent PK data well, so we sought to evaluate this model using human PK data. The most reliable human data available come from a controlled skin exposure study, but the inhalation PBPK model does not include a skin exposure route; therefore, we extended the model by incorporating compartments representing the stratum corneum and the viable epidermis and parameters that determine absorption and rate of transport through the skin. The human data revealed measurable blood concentrations of naphthalene present in the subjects prior to skin exposure, so we also introduced a continuous dose-rate parameter to account for these baseline blood concentration levels. We calibrated the three new parameters in the modified PBPK model using data from the controlled skin exposure study but did not modify values for any other parameters. Model predictions then fell within a factor of 2 of most (96%) of the human PK observations, demonstrating that this model can accurately predict internal doses of naphthalene and is thus a viable tool for use in human health risk assessment.

During the last decade, cases of West Nile Virus (WNV) have occurred in the Emilia Romagna Region (ERR). Even though the notification rates remain relatively low, ranging from 0.06 to 1.83 cases/100,000 inhabitants, the persistent pathogen’s circulation in settings characterized by favorable environmental characteristics suggests that WNV is becoming endemic to the Po River Valley. This study assesses knowledge, attitudes, and preventive practices toward WNV prevention among residents from 10 high-risk municipalities from the provinces of Parma and Reggio Emilia (total population: 82,317 inhabitants, census 2020). A web-based survey, based on the health belief model, was performed during the month of January 2021, with a convenience sampling of 469 participants from a series of closed discussion groups on social media (i.e., 2.1% of the potential responders). A total of 243 participants knew the meaning of WNV: Of them, 61.3% were aware of previous WNV infections in ERR, 76.5% acknowledged WNV infection as a severe one, but only 31.3% expressed any worry about WNV. Our results irregularly report preventive practices, either collective (e.g., draining standing water from items and the environment, 50.7%; spraying pesticides around the home, 33.0%) or individual (e.g., use of skin repellants when going outdoors, 42.6%). In a multivariate analysis, performed through binary logistic regression, participants reporting any worry towards WNV were more likely to characterize WNV as a severe disease (adjusted odds ratio [aOR] = 20.288, 95% confidence interval [CI] = 5.083–80.972). On the contrary, respondents supporting community mosquito control programs were more likely among people working with animals/livestock (aOR = 13.948, 95%CI = 2.793–69.653), and supporting tax exemptions for mosquito control programs (aOR = 4.069, 95%CI 2.098–7.893). In conclusion, our results suggest that future interventions promoting WNV prevention among residents in ERR should focus on perceptions of vulnerability to WNV, emphasizing the benefits of personal protective behaviors.

Abstract Gastrointestinal nematodes (GIN) and coccidia have a large financial and health impact on small ruminants. Recent years have seen an increase in anthelminthic/ coccidiostat resistance, and there is a need to develop alternative control methods. The objective of this study was to examine the effect of a unique proprietary compound used in the fragrance/flavor industry with an insect repellant application (Compound X; Bedoukian Research) on parasite control in sheep. Previous research by our laboratories showed a short term reduction in fecal egg count (FEC) in sheep orally drenched with the product. In the current study, naturally infected (GIN and coccidia) Katahdin lambs were orally drenched with 15 mL of Compound X and 15 mL mineral oil (CMX; n = 11) or 30 mL mineral oil (CON; n = 9) on d 0 and 21; FEC/fecal oocyst count (FOC) were determined every 7 d for 8 wk, and feces were cultured to examine GIN larvae. Data were analyzed using a mixed model with repeated measures over time, and regression (SAS). The FEC were reduced in CMX compared with CON between d 0 and 21 (P < 0.03, both mixed model and regression), d 21 and 42 (P < 0.001, mixed and regression), and d 0 and 56 (P < 0.001); FEC were influenced by day of study (P < 0.04), and the interaction tended to be significant using regression (P < 0.10). The FOC were negligible and not analyzed. There was no effect on development of nematode larvae in feces. However, there was a shift in population distribution from Haemonchus contortus to Trichostrongylus spp. which indicated a possible effect on female H. contortus fecundity. There was a sign of toxicity in one lamb following the second CMX drench. The value of using such a compound for GIN control may warrant further investigation.

Abstract. Arpiwi NL, Muksin IK, Kartini NL. 2020. Essential oil from Cymbopogon nardus and repellant activity against Aedes aegypti. Biodiversitas 21: 3873-3878. Cymbopogon nardus (L.) Rendle is an aromatic grass from the Poaceae family that produces essential oil mainly in the leaves. The essential oil from C. nardus is known as citronella oil has repellent activity. This research aimed to quantify the oil content of C. nardus leaves, to analyze the essential oil quality, to analyze the repellent activity of lotion with different concentrations of citronella oil against Aedes aegypti, and to examine the quality of formulated lotions. The oil was extracted from leaves using steam distillation, and the quality was examined according to the Indonesian National Standard. The citronella oil was formulated into a lotion with five concentrations (0, 2, 3, 4, 5% w/w). Repellent activity of the lotions against Ae. aegypti was tested using screened cage test method under laboratory conditions. Lotion quality was analyzed for pH, viscosity, homogeneity, and sensorial assessments. The result showed that the oil yield of C. nardus leaves was 0.9% w/w. The color of the oil was pale yellow, specific weight was 0.8819, the refraction index was 1.464, total geraniol was 72.71%, citronellal content was 49.14%, and solubility in 80% alcohol 1:2 was clear. The repellent activity of lotions with citronella oil was concentration-dependent, where a concentration of 5% gave the highest protection. The quality of formulated lotions met the standard and the lotions were nonirritant.

Abstract Background Gelatinous zooplankton can be difficult to preserve morphologically due to unique physical properties of their cellular and acellular components. The relatively large volume of mesoglea leads to distortion of the delicate morphology and poor sample integrity in specimens prepared with standard aldehyde or alcohol fixation techniques. Similar challenges have made it difficult to extend standard laboratory methods such as in situ hybridization to larger juvenile ctenophores, hampering studies of late development. Results We have found that a household water repellant glass treatment product commonly used in laboratories, Rain-X®, alone or in combination with standard aldehyde fixatives, greatly improves morphological preservation of such delicate samples. We present detailed methods for preservation of ctenophores of diverse sizes compatible with long-term storage or detection and localization of target molecules such as with immunohistochemistry and in situ hybridization and show that this fixation might be broadly useful for preservation of other delicate marine specimens. Conclusion This new method will enable superior preservation of morphology in gelatinous specimens for a variety of downstream goals. Extending this method may improve the morphological fidelity and durability of museum and laboratory specimens for other delicate sample types.

AbstractThe evolutionarily conserved Roundabout (Robo) family of axon guidance receptors control midline crossing of axons in response to the midline repellant ligand Slit in bilaterian animals including insects, nematodes, and vertebrates. Despite this strong evolutionary conservation, it is unclear whether the signaling mechanism(s) downstream of Robo receptors are similarly conserved. To directly compare midline repulsive signaling in Robo family members from different species, here we use a transgenic approach to express the Robo family receptor SAX-3 from the nematode Caenorhabditis elegans in neurons of the fruit fly, Drosophila melanogaster. We examine SAX-3’s ability to repel Drosophila axons from the Slit-expressing midline in gain of function assays, and test SAX-3’s ability to substitute for Drosophila Robo1 during fly embryonic development in genetic rescue experiments. We show that C. elegans SAX-3 is properly translated and localized to neuronal axons when expressed in the Drosophila embryonic CNS, and that SAX-3 can signal midline repulsion in Drosophila embryonic neurons, although not as efficiently as Drosophila Robo1. Using a series of Robo1/SAX-3 chimeras, we show that the SAX-3 cytoplasmic domain can signal midline repulsion to the same extent as Robo1 when combined with the Robo1 ectodomain. We show that SAX-3 is not subject to endosomal sorting by the negative regulator Commissureless (Comm) in Drosophila neurons in vivo, and that peri-membrane and ectodomain sequences are both required for Comm sorting of Drosophila Robo1.

Collisions between animals and motor vehicles are frequent and often result in animal mortality. In Australia, macropods are regular victims of these collisions. This has serious implications for animal welfare and conservation as well as aesthetics and tourism. Collisions with large animals and secondary collisions caused by the presence of animals on road easements, can lead to serious personal injury and property damage. A range of mitigative measures to prevent animal-vehicle collisions exists, but no single measure can be fully effective and the efficacy of many mitigation measures remains untested. An integrated management approach, employing many mitigative techniques is required to reduce vehicle-animal collisions. Repellents have recently been identified as a potential mitigative measure for reducing vehicle-animal collisions. The aim of this study was to identify the potential role of repellents in reducing macropod-vehicle collisions in New South Wales. This required the identification and assessment of potential repellents since research investigating repellents in an Australian context is scant. Macropus rufogriseus banksianus was selected as a test species for this research as a high abundance of this species exists in southeastern Australia and it is a common victim of roadkill in New South Wales. Preliminary screening trials of four potential macropod repellents highlighted the utility of two of the substances: Plant Plus, a synthetic compound based on the chemistry of dog urine; and a formulation consisting of chicken eggs. Feeding by M. rufogriseus banksianus was significantly reduced when these substances were applied near feed trays. Modest results were also detected for Δ3-isopentenyl methyl sulfide (a constituent of fox urine), while a commercial animal repellent (SCAT® Bird and Animal Repellent) was ineffective in altering feeding by M. rufogriseus banksianus. A barrier trial conducted with the two most successful repellents indicated that Plant Plus was a more effective macropod repellent then the egg formulation. Plant Plus displayed qualities of an area repellent and elicited a stronger response from M. rufogriseus banksianus when compared to the egg formulation. Further captive trials determined that the habituation of response to Plant Plus by M. rufogriseus banksianus was minimal after six weeks of constant exposure and Plant Plus retained repellent properties after exposure to ambient environmental conditions for at least ten weeks. Field trials to establish the effectiveness of Plant Plus with free ranging macropods (M. rufogriseus banksianus and M. giganteus) were unsuccessful due to methodological limitations stemming from high background variance in observed responses, equipment failure and site disturbance from outside influences. The potential role of Plant Plus as a repellent for managing macropod-vehicle collisions was highlighted by the captive trials. However, several factors requiring further research were identified. This included assessing the repellent abilities of Plant Plus in the field and further defining the properties of Plant Plus with captive trials. The effects of Plant Plus on non-target species and an assessment of potential environmental impacts also requires attention. Research assessing the potential role of repellents in other management contexts in Australia would be beneficial and the identification and assessment of repellents for other species should proceed. However, in the context of assessing repellents for use in the management of vehicle-macropod collisions, immediate focus should concentrate on extending the research to assess the effects of Plant Plus with other species of large macropod, and assessing if Plant Plus can reduce the numbers of macropods in road easements.

Tools to use for diagnosing plant disorders include overall plant appearance, plant tissue testing, total plant analysis, soil testing and analysis, and soil and root abnormalities. Plant appearance will show animal damage, weather-induced problems, chemical injuries, mechanical damage, biotic-associated problems, and plant nutrient deficiency and toxicity symptoms. Many plant growth problems can be correctly diagnosed by skillfully examining the outward appearance of a plant. By knowing the appearance of a healthy plant, one can know what would be different to cause a plant disorder. Animals can damage plants in a variety of ways. Large animals, such as deer, squirrels, gophers, moles, mice, often graze on plant tops, may break off stems, or pull the plants out of the ground. These animals can be discouraged by electric or regular fencing or by placing some repellents close to the plants. Deer can be repelled by hanging small bars of odiferous deodorant soap on the plants; or by spraying the plants with a mixture of an egg in a bucket of water. They also do not like baler twine soaked in spent soil from automobiles. Rodents often live in mulch near trees and shrubs and feed on the roots or tender shoots sometimes killing the plants. Prevention of this kind of damage can be accomplished by placing a ring of gravel or hardware cloth around the shrubs or trees to discourage this feeding. Birds also can be a problem. Woodpeckers and sapsuckers may dig holes in trees looking for insects. By keeping your trees healthy, these birds are discouraged. Other birds are often attracted to new seedings. If shrubs or small trees are damaged by birds, netting can be used to cover the plants as a final resort. Dogs also can damage plantings, usually by urinating on them. There are repellants that can be used to discourage this. Man can cause damage to plants through accidents, neglect, or ignorance as to proper care. There are a number of ways that plants can be damaged mechanically, such as root damage, trunk damage, or leaf damage, usually resulting from accidents.