Dissertations / Theses on the topic 'Angiostrongylus cantonensis'
To see the other types of publications on this topic, follow the link: Angiostrongylus cantonensis.
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Angiostrongylus cantonensis.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Ramos, Raquel Rocha. "Estudo sobre moléculas com atividade hemoglobinolítica em Angiostrongylus costaricensis e Angiostrongylus cantonensis." Pontifícia Universidade Católica do Rio Grande do Sul, 2009. http://hdl.handle.net/10923/1300.
Full textAbstract:
Made available in DSpace on 2013-08-07T18:41:29Z (GMT). No. of bitstreams: 1
000411825-Texto+Completo-0.pdf: 1068093 bytes, checksum: 29a9720f1c8599bf5e5b990d17e4dcb6 (MD5)
Previous issue date: 2009The two main species in the genus Angiostrongylus that cause human disease are A. cantonensis and A. costaricensis. These parasites have different tissue tropism, A. cantonesis is neurotropic and causes eosinophilic meningoencephalitis, A. costaricensis is located in the mesentery causing abdominal angiostrongyliasis. Immunological tests currently used for angiostrongyliasis diagnosis are limited by low especificity. Otherwise, specialized functional proteins, such as enzymes, may lead to more specific reactivity. The aim of the present work is to identify hemoglobinotytic activity in A. cantonensis. Digestive organs from the female worms were homogenized in lyses buffer. The protein extract (AcPE) was incubated whit bovine hemoglobin (BHh) at different pH range. Zymography assay was carried out by copolimerized SDS-PAGE with either 0. 4% BHb or 0. 1% gelatin. Hemoglobin degradation was well demonstrated at an extensive pH range, from 3. 0 to 7. 0. No degradation bands were detected by zymography either with gelatin or hemoglobin as substrate. These limited data from zymography and those from pH titration may suggest that AcPE contains not a single component but a low abundance enzyme complex. The identification, characterization and clonning of molecules with hemoglobinolytic activity stays as a prioritary aim.
Angiostrongylus costaricensis e A. cantonensis são as principais espécies patogênicas para o homem no gênero Angiostrongylus. Esses parasitos tem tropismo tecidual diferentes, A. cantonensis é um parasito neurotrópico que causa a angiostrongilíase meningoencefálica e A. costaricensis localiza-se no mesentério sendo o agente etiológico da angiostrongilíase abdominal. Os testes imunológicos utilizados ultimamente para o diagnóstico das Angiostrongilíases são limitados pela baixa especificidade. Entretanto, proteínas funcionais especializadas, tais como enzimas, podem ser fontes de reatividade imunológica específica. O objetivo do presente trabalho é identificar atividade hemoglobinolítica nesses parasitos. Tubos digestivos de fêmeas foram homogeneizadas em tampão de lise. As proteínas do extrato (ExAca) foram incubadas com hemoglobina bovina (HbB) em diferentes pHs. Zimografia foi realizado em géis copolimerizados com 0,4% gelatina ou 0,1% BHb. Degradação da hemoglobina foi bem demonstrada em uma ampla faixa de pH, de 3,0 para 7,0. Não foram detectadas bandas de degradação na zimografia com gelatina ou hemoglobina como substrato. Os dados limitados da zimografia e os resultados de atividade hemoglobinolítica, com ou sem a titulação de pH, pode sugerir um complexo de proteases em pequena quantidade. Exploração de diversas estratégias de concentração do extrato protéico, sem perda da atividade da enzima, constitue a perspectiva desse trabalho, visando à identificação, caracterização e produção em larga escala de moléculas com atividade hemoglobinolítica.
2
Ben, Renata. "Ausência do endosimbionte Wolbachia SP. em dois metastrongilídeos: Angiostrongylus costaricensis e Angiostrongylus cantonensis." Pontifícia Universidade Católica do Rio Grande do Sul, 2007. http://hdl.handle.net/10923/5343.
Full textAbstract:
Made available in DSpace on 2013-08-07T19:12:21Z (GMT). No. of bitstreams: 1
000414796-Texto+Completo-0.pdf: 186239 bytes, checksum: 84d975c8024507f47eadc59f4d672996 (MD5)
Previous issue date: 2007Abdominal angiostrongyliasis is caused by Angiostrongylus costaricensis, an intra-arterial nematode, that lives in the ileocecal region in wild rodents. This parasite has been detected from southern United States to northern Argentina. Man is an accidental host and is infected ingesting third stage larvae (L3) that are eliminated with mucous secretions by the intermediate host (veronicelid slugs). Another species, that also may infect man is Angiostrongylus cantonensis, a rat pulmonary worm, responsabile for eosinophilic meningitis, in Asia and Pacific islands. Parasites of parasites are currently being studied not only to open new therapeutics possibilities, but also in order to improve diagnostic techniques. The interest for Wolbachia, a gram-negative endosimbiont bacterium, increased when the mutualistic character of its association with filarias was described. These considerations led to new ideas for treatment of these parasitosis through the use of antibacterial drugs. The main objective of this work is to verify the Wolbachia sp presence in A. costaricensis and A. cantonensis, and study its contribution for the humoral immune response of the vertebrate host. The first step was to look for evidences in favor of the presence of Wolbachia sp. Nucleic acids, through the PCR technique. In some experiments amplification products were obtained, what could be an indication of the presence of the bacterium, but these data must be confirmed by electronic microscopy and immunohistology. Because of the difficulties to get Wolbachia sp. antigen, alternatively serum samples from individuals with abdominal angiostrongyliasis were tested against Escherichia coli antigen, because it is a common bacterium species in vertebrates’ microbiota that could eventually colonize the worm. Through a western-blot analysis it was clearly demonstrated a reactivity not exclusively associated to A. costaricensis’ infected individuals. Moreover, fragments of worms were introduced in bacterial culture medium in order to study the microbiota of the Angiostrongylus adult worm. The fact of being found only one species of a grampositive bacillum in this experiment seems to confirm the hypothesis that in intravascular environment, with a low tolerance for bacteria, the worm’s microbiota is reduced in number and diversity. From the experiments we were not able to identify the presence of Wolbachia sp neither in A. costaricensis nor in A. cantonensis. It remains open to further investigations the contribution of other bacteria or simbionts of helminthes, for antigenic recognition by the vertebrate host, with potential implications for diagnosis, pathogenesis and treatment
A angiostrongilíase abdominal é causada pelo Angiostrongylus costaricensis, um nematódeo intra-arterial, que vive na região íleo-cecal de roedores silvestres. Esta parasitose tem sido registrada desde o sul dos Estados Unidos até o norte da Argentina. O homem é hospedeiro acidental e se infecta ingerindo as larvas de terceiro estágio (L3) presentes no muco do hospedeiro intermediário (veronicelídeos). Outra espécie, que também é parasita do homem, é Angiostrongylus cantonensis, um verme pulmonar de ratos, causador da meningite eosinofílica, que ocorre na Ásia e ilhas do Pacífico. Parasitas de parasitas são atualmente alvo de estudos não somente para abrir novas possibilidades terapêuticas, bem como para aprimorar técnicas diagnósticas. O interesse pela Wolbachia sp, uma bactéria gram-negativa endosimbionte, aumentou no momento em que descobriram sua característica mutualística em relação à filária. Estas considerações levaram a novas idéias para o tratamento destas parasitoses através da utilização de drogas antibacterianas. O objetivo principal deste trabalho é verificar a presença de Wolbachia em A. costaricensis e em A. cantonensis, e estudar a sua contribuição para a resposta imune humoral do hospedeiro vertebrado. O primeiro passo foi buscar evidências da presença de ácidos nucléicos de Wolbachia, através da técnica de PCR. Em alguns experimentos foram obtidos produtos de amplificação, o que poderia ser um indício da presença da bactéria, mas esses dados devem ser confirmados por microscopia eletrônica e por imunohistologia. Diante das dificuldades para se obter antígeno de Wolbachia sp alternativamente amostras de soro de indivíduos com angiostrongilíase foram testados contra antígenos de Escherichia coli por ser uma bactéria comum na microbiota de vertebrados e que eventualmente poderia colonizar o verme. Através da análise por imunoeletrotransferência ficou claramente demonstrada uma reatividade não relacionada exclusivamente aos indivíduos infectados por A. costaricensis. Além disso, fragmentos de vermes foram semeados em meio de cultura a fim de estudar a microbiota do verme adulto de Angiostrongylus. O fato de só ser encontrada um bacilo gram positivo nesse experimento parece confirmar a hipótese de que, por ser o ambiente intravascular pouco tolerante à presença de bactérias, a microbiota do verme deve ser pouco numerosa e diversa. Permanece aberta para futuras investigações a contribuição de outras bactérias ou outros simbiontes em helmintos, para reconhecimento antigênico pelo hospedeiro vertebrado, com possíveis implicações para diagnóstico, patogenia e tratamento.
3
Paula, Alessandra Teixeira de. "Controle biológico de Angiostrongylus cantonensis utilizando fungos nematófagos." Universidade Federal de Viçosa, 2014. http://locus.ufv.br/handle/123456789/5187.
Full textAbstract:
Made available in DSpace on 2015-03-26T13:47:24Z (GMT). No. of bitstreams: 1
texto completo.pdf: 812202 bytes, checksum: b8af5169f54eccc705c53128fef2ba2b (MD5)
Previous issue date: 2014-02-19The parasitosis, in a medical and social standpoint, presents important public health problems. In Brazil, an average of a third of population lives in areas with risk of transmission and occurrence of parasitic diseases. The lack of basic sanitation, as well as environmental factors particular of tropical climates, inequality of the socioeconomic conditions of the individuals and the lack of information are factors that favor its occurrence. The colonization process, establishment of new routes of trade and human migration, contributed to the introduction of pathogens transported by their hosts to other regions. This way, various species found out of their origin area. The parasite Angiostrongylus cantonensis and the exotic snail Achatina fulica are two examples of organisms that were introduced in Brazil. A.fulica is one of the intermediate hosts of the parasite A. cantonensis, responsible for cause in humans, disease known as eosinophilic meningoencephalitis, may lead parasitized individuals to death. In various regions of the country, was confirmed the presence of this parasite naturally occurring, as well as cases of infected humans. This way, measures of alternative control, are important to avoid its dispersion. In this context was evaluated the predatory capability of eight fungal isolates belong to the species Duddingtonia flagrans (isolated: AC001, CG768 and CG722), Monacrosporium thaumasium (isolated: NF34), M. sinense (isolated: SF53) and Arthobotrys robusta (isolated: I31), A. cladodes (isolated: CG719) and A. conoídes (isolated: I40) on first larvae stage (L1) of A. cantonensis in laboratory conditions. Were formed nine groups: eight groups treated with the different isolated and one control group (without fungi). The groups treated contained 1000 conidia of isolated fungal and 1000 L1 of A. cantonensis in Petri dish containing Agar- water 2% (AA2%). The control group contained only 1000 L1 of A. cantonensis in AA2%. Evidence of predatory activity of all fungal isolates tested could be observed at the end of seven days of the experiment through the recovery of ixnon-predated L1, where were observed the following percentage of reduction of L1 with the respective isolates: AC001 (82,8%); CG768 (71,0%); CG722 (72,8%); NF34 (86,7%), SF53 (89,7%); I40 (48,3%), CG719 (84,7%) and I31 (80,4%). No difference was observed (p>0,01) between the action of the isolated used in the end of seven days, however, was observed difference (p<0,01) compared with the control group. The results observed demonstrated that the fungi belonged to the genus Duddingtonia (AC001, CG768 and CG722), Monacrosporium (NF34 and SF53) and Arthrobotrys (I31 and CG719), could be used in biological control, once that these were capable of reduce the population of larvae (L1) of A. cantonensis in vitro.
As parasitoses, do ponto de vista médico e social, representam importantes problemas de saúde pública. No Brasil, em média um terço da população vive em áreas com risco de transmissão e ocorrência de doenças parasitárias. A falta de saneamento básico, bem como fatores ambientais próprios de clima tropical, desigualdade das condições socioeconômicas dos indivíduos e a falta de informação favorecem sua ocorrência. Os processos de colonização, estabelecimento de novas rotas de comércio e migração humana, contribuíram para a introdução de patógenos transportados por seus hospedeiros para outras regiões. Dessa forma, várias espécies se encontram fora de sua área de origem. O parasito Angiostrongylus cantonensis e o caramujo exótico Achatina fulica, são dois exemplos de organismos que foram introduzidos no Brasil. A. fulica é um dos hospedeiros intermediários do parasito A. cantonensis, responsável por causar em humanos, doença conhecida como meningoencefalite eosinofilica, podendo levar indivíduos parasitados a óbito. Em várias regiões do país, foi confirmada a presença deste parasito ocorrendo naturalmente, bem como casos de humanos infectados. Dessa forma, medidas de controle alternativo, são importantes para evitar sua dispersão. Nesse contexto foi avaliada a capacidade predatória de oito isolados fúngicos pertencentes ás espécies Duddingtonia flagrans (isolados: AC001, CG768 e CG722), Monacrosporium thaumasium (isolado: NF34), M. sinense (isolado: SF53) e Arthobotrys robusta (isolado: I31), A. cladodes (isolado: CG719) e A. conoídes (isolado: I40) sobre larvas de primeiro estádio (L1) de A. cantonensis em condições laboratoriais. Foram formados nove grupos: oito grupos tratados com os diferentes isolados, e um grupo controle (sem fungos). Os grupos tratados continham 1000 conídios dos isolados fúngicos e 1000 L1 de A. cantonensis em placa de Petri contendo o meio Agar-água 2% (AA2%). O viigrupo controle continha apenas 1000 L1 de A. cantonensis em AA2%. Evidências da atividade predatória de todos os isolados fúngicos testados, foram observadas ao final de sete dias do experimento, através da recuperação das larvas (L1) não predadas, sendo observados os seguintes percentuais de redução com os respectivos isolados: AC001 (82,8%); CG768 (71,0%); CG722 (72,8%); NF34 (86,7%), SF53 (89,7%); I40 (48,3%), CG719 (84,7%) e I31 (80,4%). Não houve diferença (p>0,01) entre a ação dos isolados utilizados ao final de sete dias, no entanto, foi observado diferença (p<0,01) em relação ao grupo controle. Os resultados demonstraram que os fungos pertencentes aos gêneros Duddingtonia (AC001, CG768 E CG722), Monacrosporium (NF34 e SF53) e Arthrobotrys (I31 e CG719), poderiam ser utilizados no controle biológico, uma vez que estes foram capazes de reduzir a população de larvas (L1) de A. cantonensis in vitro.
4
Hüttemann, Maria. "Licht- und elektronenmikroskopische Untersuchungen an Entwicklungsstadien von Angiostrongylus cantonensis und Trichuris muris (Nematodes)." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=97184397X.
Full text
5
DELAFOREST, CHRISTINE. "La meningite a eosinophiles provoquee par angiostrongylus cantonensis : revue de la litterature." Lyon 1, 1990. http://www.theses.fr/1990LYO1M102.
Full text
6
Migraine, Bertrand Ghawche Frédéric. "Méningite à éosinophiles en Polynésie à propos de 17 cas /." [S.l.] : [s.n.], 2008. http://castore.univ-nantes.fr/castore/GetOAIRef?idDoc=48161.
Full text
7
Ramos, Raquel Rocha. "Estudo sobre mol?culas com atividade hemoglobinol?tica em Angiostrongylus costaricensis e Angiostrongylus cantonensis." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2009. http://tede2.pucrs.br/tede2/handle/tede/5341.
Full textAbstract:
Made available in DSpace on 2015-04-14T14:50:55Z (GMT). No. of bitstreams: 1
411825.pdf: 1068093 bytes, checksum: 29a9720f1c8599bf5e5b990d17e4dcb6 (MD5)
Previous issue date: 2009-04-03Angiostrongylus costaricensis e A. cantonensis s?o as principais esp?cies patog?nicas para o homem no g?nero Angiostrongylus. Esses parasitos tem tropismo tecidual diferentes, A. cantonensis ? um parasito neurotr?pico que causa a angiostrongil?ase meningoencef?lica e A. costaricensis localiza-se no mesent?rio sendo o agente etiol?gico da angiostrongil?ase abdominal. Os testes imunol?gicos utilizados ultimamente para o diagn?stico das Angiostrongil?ases s?o limitados pela baixa especificidade. Entretanto, prote?nas funcionais especializadas, tais como enzimas, podem ser fontes de reatividade imunol?gica espec?fica. O objetivo do presente trabalho ? identificar atividade hemoglobinol?tica nesses parasitos. Tubos digestivos de f?meas foram homogeneizadas em tamp?o de lise. As prote?nas do extrato (ExAca) foram incubadas com hemoglobina bovina (HbB) em diferentes pHs. Zimografia foi realizado em g?is copolimerizados com 0,4% gelatina ou 0,1% BHb. Degrada??o da hemoglobina foi bem demonstrada em uma ampla faixa de pH, de 3,0 para 7,0. N?o foram detectadas bandas de degrada??o na zimografia com gelatina ou hemoglobina como substrato. Os dados limitados da zimografia e os resultados de atividade hemoglobinol?tica, com ou sem a titula??o de pH, pode sugerir um complexo de proteases em pequena quantidade. Explora??o de diversas estrat?gias de concentra??o do extrato prot?ico, sem perda da atividade da enzima, constitue a perspectiva desse trabalho, visando ? identifica??o, caracteriza??o e produ??o em larga escala de mol?culas com atividade hemoglobinol?tica.
8
鄺懿珩 and Yi-hang Agnes Kwong. "Lipid composition and lipases of Angiostrongylus cantonensis (nematoda: metastrongyloidea)." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1989. http://hub.hku.hk/bib/B31209208.
Full text
9
蘇雅頌 and Ngar-chung Nellie So. "Pyrimidine nucleotide biosynthesis in adult angiostrongylus Cantonensis (Nematoda : Metastrongyloidea)." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1993. http://hub.hku.hk/bib/B3123320X.
Full text
10
So, Ngar-chung Nellie. "Pyrimidine nucleotide biosynthesis in adult angiostrongylus Cantonensis (Nematoda : Metastrongyloidea) /." [Hong Kong : University of Hong Kong], 1993. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13637745.
Full text
11
Kwong, Yi-hang Agnes. "Lipid composition and lipases of Angiostrongylus cantonensis (nematoda: metastrongyloidea) /." [Hong Kong : University of Hong Kong], 1989. http://sunzi.lib.hku.hk/hkuto/record.jsp?B12583273.
Full text
12
Ben, Renata. "Aus?ncia do endosimbionte Wolbachia SP. em dois metastrongil?deos : Angiostrongylus costaricensis e Angiostrongylus cantonensis." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2007. http://tede2.pucrs.br/tede2/handle/tede/169.
Full textAbstract:
Made available in DSpace on 2015-04-14T13:09:12Z (GMT). No. of bitstreams: 1
414796.pdf: 186239 bytes, checksum: 84d975c8024507f47eadc59f4d672996 (MD5)
Previous issue date: 2007-03-30A angiostrongil?ase abdominal ? causada pelo Angiostrongylus costaricensis, um nemat?deo intra-arterial, que vive na regi?o ?leo-cecal de roedores silvestres. Esta parasitose tem sido registrada desde o sul dos Estados Unidos at? o norte da Argentina. O homem ? hospedeiro acidental e se infecta ingerindo as larvas de terceiro est?gio (L3) presentes no muco do hospedeiro intermedi?rio (veronicel?deos). Outra esp?cie, que tamb?m ? parasita do homem, ? Angiostrongylus cantonensis, um verme pulmonar de ratos, causador da meningite eosinof?lica, que ocorre na ?sia e ilhas do Pac?fico. Parasitas de parasitas s?o atualmente alvo de estudos n?o somente para abrir novas possibilidades terap?uticas, bem como para aprimorar t?cnicas diagn?sticas. O interesse pela Wolbachia sp, uma bact?ria gram-negativa endosimbionte, aumentou no momento em que descobriram sua caracter?stica mutual?stica em rela??o ? fil?ria. Estas considera??es levaram a novas id?ias para o tratamento destas parasitoses atrav?s da utiliza??o de drogas antibacterianas. O objetivo principal deste trabalho ? verificar a presen?a de Wolbachia em A. costaricensis e em A. cantonensis, e estudar a sua contribui??o para a resposta imune humoral do hospedeiro vertebrado. O primeiro passo foi buscar evid?ncias da presen?a de ?cidos nucl?icos de Wolbachia, atrav?s da t?cnica de PCR. Em alguns experimentos foram obtidos produtos de amplifica??o, o que poderia ser um ind?cio da presen?a da bact?ria, mas esses dados devem ser confirmados por microscopia eletr?nica e por imunohistologia. Diante das dificuldades para se obter ant?geno de Wolbachia sp alternativamente amostras de soro de indiv?duos com angiostrongil?ase foram testados contra ant?genos de Escherichia coli por ser uma bact?ria comum na microbiota de vertebrados e que eventualmente poderia colonizar o verme. Atrav?s da an?lise por imunoeletrotransfer?ncia ficou claramente demonstrada uma reatividade n?o relacionada exclusivamente aos indiv?duos infectados por A. costaricensis. Al?m disso, fragmentos de vermes foram semeados em meio de cultura a fim de estudar a microbiota do verme adulto de Angiostrongylus. O fato de s? ser encontrada um bacilo gram positivo nesse experimento parece confirmar a hip?tese de que, por ser o ambiente intravascular pouco tolerante ? presen?a de bact?rias, a microbiota do verme deve ser pouco numerosa e diversa. Permanece aberta para futuras investiga??es a contribui??o de outras bact?rias ou outros simbiontes em helmintos, para reconhecimento antig?nico pelo hospedeiro vertebrado, com poss?veis implica??es para diagn?stico, patogenia e tratamento.
13
Garcia, Juberlan Silva. "Avaliação bioquímica, hematológica e histopatológica da infecção experimental por Angiostrongylus cantonensis no hospedeiro definitivo Rattus norvegicus (Wistar) e estudo da interação Angiostrongylus cantonensis / Echinostoma paraensei no hospedeiro intermediário Biomphalaria glabrata." reponame:Repositório Institucional da FIOCRUZ, 2014. https://www.arca.fiocruz.br/handle/icict/13049.
Full textAbstract:
Made available in DSpace on 2016-03-08T14:05:17Z (GMT). No. of bitstreams: 2
juberlan_garcia_ioc_dout_2014.pdf: 5272747 bytes, checksum: 0a5731978e1e998b6728ba6318bbdb14 (MD5)
license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)
Previous issue date: 2016-02-23A meningite eosinofílica é uma doença caracterizada pelo aumento de eosinófilos no líquido cefalorraquidiano (LCR), cuja causa mais comum é a invasão do sistema nervoso central por helmintos. Entre os helmintos, destaca-se o Angiostrongylus cantonensis. O roedor Rattus norvegicus é o hospedeiro natural definitivo e o homem atua como hospedeiro acidental e que pode se infectar através da ingestão de moluscos crus ou mal cozidos ou alimentos contaminados com a larva L3. Recentemente, no Brasil houve quatro casos de meningite eosinofílica, devido à ingestão ou manipulação de Achatina fulica infectada. Foi realizado uma avaliação das alterações metabólicas, fisiológicas e histopatológicas em R. norvegicus experimentalmente infectados com 100 larvas L3 de A. cantonensis e o estudo do efeito da infecção concomitante por Echinostoma paraensei e A. cantonensis sobre a atividade das aminotransferases ALT e AST, na concentração de proteínas totais, ácido úrico, ureia e análise histopatológica para compreender o efeito da infecção concomitante em Biomphalaria glabrata Os resultados obtidos demonstraram que a infecção causada por A. cantonensis em R. norvegicus promoveu alterações metabólicas hepáticas e histopatológicas, caracterizadas pelo aumento da atividade das enzimas ALT, AST, GGT e ALKP, alteração no metabolismo glicídico, presença de infiltrado inflamatório, alterações hematológicas significativas, além disso promoveu alterações histopatológicas no parênquima pulmonar demonstraram nos roedores a ocorrência de áreas de necrose e extensa fibrose, podendo estar diretamente relacionada com o desenvolvimento de hipóxia celular. Os moluscos infectados apresentaram alterações nos níveis das aminotransferases e aumento na excreção de produtos nitrogenados, os resultados histopatológicos mostraram uma mudança na distribuição de A. cantonensis na presença de E. paraensei, indicando que a presença deste trematódeo pode interferir na dinâmica do A. cantonensis. A infecção por A. cantonensis causou alterações metabólicas e histopatológicas nos roedores
E osinophilic meningitis is a disease characterized by increased eosinophils in the cerebrospinal fluid (CSF), which is the most common ly cause d by invasion of the central nervous system by helminths , as occurs in Angiostrongylus cantonensis infections . The rodent Rattus norvegicus is the definitive natural host and humans act as accidental host s and can become infected by eating raw or undercooked snail s or food contaminated with infective L 3 larvae. Recently in Brazil there have been four cases of eosinophilic meningitis due to ingestion of infected Achatina fulica . An evaluation of metabolic, p hysiological and pathological changes in R. norvegicus experimentally infected with 100 L 3 larvae of A. cantonensis and study the effect of co - infection with Echinostoma paraensei and A. cantonensis on the activity of aminotransferases ALT and AST was perf ormed in the concentration total protein, uric acid, urea and histopathologic analysis to understand the effect of co - infection in Biomphalaria glabrata . The results showed that the infection caused by A. cantonensis in R. norvegicus promoted histopathological changes in liver metabolism is characterized by increased activity of ALT, AST, GGT and ALKP enzymes in glucose metabolism, inflammatory infiltrate, hematological changes significant in addition in addition histopathological alt erations in lung parenchyma in rodents have shown the occurrence of extensive areas of necrosis and fibrosis, which can be directly related to the development of cellular hypoxia. The infected snails showed altered levels of aminotransferases and increased excretion of nitrogen products, histopathological findings showed a change in the distribution of A. cantonensis in the presence of E. paraensei, indicating that the presence of this trematode may interfere with the dynamics of A. cantonensis .
14
Veríssimo, Carolina de Marco. "Estudo de glicídios de Angiostrongylus cantonensis e o papel no imunodiagnóstico." Pontifícia Universidade Católica do Rio Grande do Sul, 2015. http://hdl.handle.net/10923/8310.
Full textAbstract:
Made available in DSpace on 2016-06-02T12:30:26Z (GMT). No. of bitstreams: 1
000478795-Texto+Completo-0.pdf: 3480923 bytes, checksum: c145d85afafb9cc7cd5782a17ac34cfb (MD5)
Previous issue date: 2015Angiostrongylus cantonensis is a nematode parasite, main etiologic agent of the eosinophilic meningitis (EM) in humans, a disease endemic in many tropical and sub tropical countries. The diagnosis of EM involves clinical evaluation, eosinophils counting >10% in liquor, and consuming historical of raw mollusks, as they are the intermediate hosts of the Angiostrongylus. Definitive diagnosis through larvae visualization in the liquor is rare. Serological test, mainly involving the 31-kDa component detection, which presents high sensitivity and specificity, has been employed as an alternative way for diagnostic. The 31-kDa antigen is composed for glycoproteins and tentatives in producing it in a recombinant way, using either prokaryotic or eukaryotic models, were inable to mantain immunological recognition, probably due the lack or deficient glycosilation of the molecules. Due the lack of information about A. cantonensis glycans and the need of producing a standard antigen able to be used worldwide in a diagnostic test, the main goal of this work was to study the A. cantonensis glycan profile and their role on the immune diagnosis of EM. It was used total soluble extract (TE) and excretory-secretory products (ES) as sources of glycans and glycoconjugates. The N-linked glycans and glycoconjugates from A. cantonensis female TE and 31-kDa antigen were analyzed and identified by mass spectrometry (MS) and lectin array, and the immunogenecity of these molecules were characterized by dot blot and Western blots. Furthermore, It was investigated the biosynthesis routes of glycans using in silico analysis of the Angiostrongylus genome and transcriptome dataset. N-glycans containing complex structures, with truncated antennas containing terminal with galactose and N-acetylgalactosamine, and core α1-6 fucosylated were identified. Lectin array analysis could also dentify Gal and GalNAc structures in Angiostrongylus glycoconjugates. Eight genes involved with biosynthesis of N-glycans, among them GCS1; GANAB, MAN1, MGAT2 and FUT8; and three involved with O-glycan biosynthesis, GALNT, C1GALT1 e OFUT1 were found by in silico analysis. Immunogenicity of the 31-kDa antigen is tottaly dependent of N-glycans and not to O-glycans. Modeling of proteins of the 31kDa component showed N-glycosilation sites and predicted structures that were the same identified by MS analysis. Taking together, the data generated in this study shown the glycan importance for angiostrongyliasis diagnosis and also the glycan repertoire that Angiostrongylus produces. This work is an important contribution to the development of a standard diagnosis for EM and also for new perspectives in the study of angiostrongyliasis diagnosis, parasite biology and host-parasite relationship.
Angiostrongylus cantonensis é um nematódeo parasita, principal agente etiológico da meningite eosinofílica (ME) em humanos, doença endêmica em diversos países tropicais e subtropicais. A ME se caracteriza pela eosinofilia >10% no líquor, e histórico de ingestão de moluscos crus, já que estes são hospedeiros intermediários do Angiostrongylus. O diagnóstico definitivo é raramente possível através da visualização da larva no liquor. Testes sorológicos envolvendo principalmente a detecção do componente de 31-kDa, que apresenta alta sensibilidade e especificidade, tem sido empregados como uma alternativa diagnóstica. O antígeno de 31-kDa é composto por glicoproteínas e tentativas em produzi-lo de maneira recombinante, em modelos procarióticos ou eucarióticos, não tiveram sucesso em manter o reconhecimento imunológico, provavelmente pela incorreta ou insuficiente glicosilação das moléculas. Devido à falta de informação sobre glicídios de A. cantonensis e a necessidade de produzir um antígeno padrão capaz de ser usado em um teste de diagnóstico para distribuição mundial, o objetivo principal deste trabalho foi estudar o perfil glicídico de A. cantonensis e o papel dessas moléculas no imunodiagnóstico da ME. Foram utilizados extratos solúveis totais (ET) de vermes adultos machos e fêmeas e produtos de excreção e secreção (ES) como fontes dos glicídios e glicoconjugados estudados. Os N-glicídios e glicoconjugados do ET de verme fêmea de A. cantonensis e o antígeno de 31-kDa foram analisados e identificados por espectrometria de massa (EM) e lectina array, e a importância imunogênica destas moléculas foi caracterizada por tratamento com glicosidases aliado a dot blot ou Western blot. Além disso, foi investigada a presença de enzimas envolvidas na síntese de diferentes glicídios a partir de análises in silico do genoma e transcriptoma do Angiostrongylus. Diversos N-glicídios foram identificados nas amostras analisadas. Estes continham estruturas complexas, com antenas truncadas contendo galactose e N-acetilgalactosamina em posições terminais e núcleo α1-6 fucosilado. As mesmas estruturas terminais foram identificadas pela análise de Lectin Array. Em relação a análise in sílico da biossíntese de glicídios em Angiostrongylus, foram identificados oito genes envolvidos na síntese de N-glicídios, entre eles GCS1; GANAB, MAN1, MGAT2 e FUT8; e pelo menos três genes envolvidos na síntese de O-glicídios, GALNT, C1GALT1 e OFUT1. Os N-glicídios se mostraram essenciais para a imunogenicidade do antígeno de 31-kDa quando soros positivos para A. cantonensis foram testados. Uma modelagem in silico dos componentes de 31-kDa, demonstrou sítios de glicosilação para N-glicídios, e previu as mesmas estruturas que foram identificadas por EM. Em conjunto, os dados gerados neste estudo mostraram a importância de glicídios para o diagnóstico de angiostrongilíases e também o repertório de glicídios que este parasito pode produzir. Este trabalho é uma contribuição importante para o desenvolvimento de um diagnóstico padrão para a ME e também para novas perspectivas no estudo do diagnóstico de angiostrongilíases, biologia do parasito e relação parasito-hospedeiro.
15
Monte, Tainá Carneiro de Castro. "Estudo Filogenético, Biológico e Morfológico de isolados de Angiostrongylus cantonensis provenientes de diferentes áreas geográficas do Brasil." reponame:Repositório Institucional da FIOCRUZ, 2014. https://www.arca.fiocruz.br/handle/icict/12867.
Full textAbstract:
Made available in DSpace on 2016-02-26T13:38:42Z (GMT). No. of bitstreams: 2
taina_monte_ioc_mest_2014.pdf: 2518433 bytes, checksum: 7691ab89b672f7a5b9084cbb9979957c (MD5)
license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5)
Previous issue date: 2016-02-23Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
Angiostrongylus cantonensis é responsável por causar meningoencefalite eosinofílica em humanos e casos já foram registrados em diversas partes do mundo incluindo o Brasil (ES, PE e SP). Nesse estudo, relatamos a variabilidade genética entre isolados de A. cantonensis do Brasil utilizando sequências do gene mitocondrial COI. Foram identificados três haplótipos brasileiros de A. cantonensis, baseados em oito haplótipos conhecidos (ac1-ac8). O haplótipo brasileiro ac5 ficou agrupado com isolados do Japão e o haplótipo brasileiro ac8 (isolados do RJ, SP, PA e PE) formaram um clado distinto. Foi relatado um novo haplótipo brasileiro, haplótipo ac9, o qual se encontra intimamente relacionado com os haplótipos da China (ac6) e do Japão (ac7). Dois isolados brasileiros de A. cantonensis, Olinda e Caju (haplótipos ac8 e ac9, respectivamente) relatados no presente estudo, tiveram sua biologia e morfologia caracterizadas após infecção experimental. Foi observada diferença significativa com maior carga parasitária recuperada nos isolados de Caju e um número significativamente maior de larvas L1 eliminadas nas fezes no início do período patente. Entretanto, quando comparado o total de larvas eliminadas não foi verificada diferença significativa entre os dois isolados O isolado de Caju apresentou diferença significativa na proporção entre espécimes fêmeas e machos (0,64:1), enquanto que o mesmo não foi observado para o isolado de Olinda (1,16:1). A análise morfométrica revelou que os espécimes machos e fêmeas do isolado de Olinda foram significativamente maiores com relação aos caracteres analisados quando comparados com os espécimes de Caju. A análise morfológica evidenciou pequena variação no nível das bifurcações que unem os raios laterais no lobo direito da bolsa copuladora, entre os dois isolados. A variação genética observada apoia a hipótese que o aparecimento do parasito no Brasil é um resultado de múltiplas introduções de ratos parasitados e pelo molusco Achatina fulica, o qual contribui para a dispersão. As variações biológicas, morfológicas e morfométricas entre os dois haplótipos estudados, reforçam a variação observada pelo marcador COI e pela possível influência do isolamento geográfico. Estudos futuros devem ser realizados para verificar a possível presença do haplótipo recentemente relatado, ac9, em outras áreas do país, além da zona portuária da cidade do Rio de Janeiro
Angiostrongylus cantonensis is respons ible for causing eosinophili c meningoencephalitis in humans and cases have been recorded in various part s of the world including Brazil (ES, PE and SP) . In this study, we report the geneti c variability among Brazilian isolates of A. cantonensis using seque nces of the mitochondrial COI gen e . We identified three Brazilian haplotypes of A. cantonensis , based o n eight known haplotypes (ac1 - ac8) . The Brazilian haplotype ac 5 , was clustered with isolates from Japan and the Brazilian haplotype ac8 ( is olates from R J, SP, PA and PE) formed a distinct clade . It was reported a new Brazilian haplotype , haplotype ac9 , which is closely re lated to haplotype from China (ac6) and Japan (ac7) . Two Brazilian isolates of A. cantonensis , Olinda and Caju (haplotypes ac8 and ac9 , respectively ) reported in this st udy, had their biology and morphology characterized after experimental infection . Significant differences were observed with higher parasite load recovered in the isolates from Caju and a significantly greater number of L1 larvae eliminated in the feces at the beg inning of the patent pe riod . However , when compared to the total larvae eliminated there was no significant difference between the two isolates . The isolates from Caju showed significant difference in the proportion of female and male spec imens (0,64 :1) , but it was not observe d for isolates from Olinda (1,16 :1 ) . The m orphom etric analysis showed that male and female spec imens from Olinda were significantly higher with respect to the analyzed characters when c ompared w it h specimens from Caju. The m orphologic al analysis showed little variation in the level of bifurcations that unite the lateral rays in the right lobe of copulatory bursa , betwe en the two isolates . Genetic variation among isolates supports the hypothesis t hat the appearance of the parasite in Brazil is a result of multiple introd uctions of infected rodents and by the mollusc , Achatina fulica , which contributes to the dispersion . Biological, morphological and morphometric variation between the two haplotype s of A. cantonensis studied , reinforce the observed v ariation by the COI marker and the possible influence of geographical isolation. Future studies should be performed to verify the possible presence of the haplotype recently reported, ac 9, in other areas of the country, beyond the port area of the city of Rio de Janeiro.
16
Ver?ssimo, Carolina De Marco. "Estudo de glic?dios de Angiostrongylus cantonensis e o papel no imunodiagn?stico." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2015. http://tede2.pucrs.br/tede2/handle/tede/6711.
Full textAbstract:
Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-05-30T16:54:35Z
No. of bitstreams: 1
TES_CAROLINA_DE_MARCO_VERISSIMO_COMPLETO.pdf: 3480923 bytes, checksum: c145d85afafb9cc7cd5782a17ac34cfb (MD5)Made available in DSpace on 2016-05-30T16:54:35Z (GMT). No. of bitstreams: 1 TES_CAROLINA_DE_MARCO_VERISSIMO_COMPLETO.pdf: 3480923 bytes, checksum: c145d85afafb9cc7cd5782a17ac34cfb (MD5) Previous issue date: 2015-08-31
Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq
Angiostrongylus cantonensis is a nematode parasite, main etiologic agent of the eosinophilic meningitis (EM) in humans, a disease endemic in many tropical and sub tropical countries. The diagnosis of EM involves clinical evaluation, eosinophils counting >10% in liquor, and consuming historical of raw mollusks, as they are the intermediate hosts of the Angiostrongylus. Definitive diagnosis through larvae visualization in the liquor is rare. Serological test, mainly involving the 31-kDa component detection, which presents high sensitivity and specificity, has been employed as an alternative way for diagnostic. The 31-kDa antigen is composed for glycoproteins and tentatives in producing it in a recombinant way, using either prokaryotic or eukaryotic models, were inable to mantain immunological recognition, probably due the lack or deficient glycosilation of the molecules. Due the lack of information about A. cantonensis glycans and the need of producing a standard antigen able to be used worldwide in a diagnostic test, the main goal of this work was to study the A. cantonensis glycan profile and their role on the immune diagnosis of EM. It was used total soluble extract (TE) and excretory-secretory products (ES) as sources of glycans and glycoconjugates. The N-linked glycans and glycoconjugates from A. cantonensis female TE and 31-kDa antigen were analyzed and identified by mass spectrometry (MS) and lectin array, and the immunogenecity of these molecules were characterized by dot blot and Western blots. Furthermore, It was investigated the biosynthesis routes of glycans using in silico analysis of the Angiostrongylus genome and transcriptome dataset. N-glycans containing complex structures, with truncated antennas containing terminal with galactose and N-acetylgalactosamine, and core ?1-6 fucosylated were identified. Lectin array analysis could also dentify Gal and GalNAc structures in Angiostrongylus glycoconjugates. Eight genes involved with biosynthesis of N-glycans, among them GCS1; GANAB, MAN1, MGAT2 and FUT8; and three involved with O-glycan biosynthesis, GALNT, C1GALT1 e OFUT1 were found by in silico analysis. Immunogenicity of the 31-kDa antigen is tottaly dependent of N-glycans and not to O-glycans. Modeling of proteins of the 31kDa component showed N-glycosilation sites and predicted structures that were the same identified by MS analysis. Taking together, the data generated in this study shown the glycan importance for angiostrongyliasis diagnosis and also the glycan repertoire that Angiostrongylus produces. This work is an important contribution to the development of a standard diagnosis for EM and also for new perspectives in the study of angiostrongyliasis diagnosis, parasite biology and host-parasite relationship.
Angiostrongylus cantonensis ? um nemat?deo parasita, principal agente etiol?gico da meningite eosinof?lica (ME) em humanos, doen?a end?mica em diversos pa?ses tropicais e subtropicais. A ME se caracteriza pela eosinofilia >10% no l?quor, e hist?rico de ingest?o de moluscos crus, j? que estes s?o hospedeiros intermedi?rios do Angiostrongylus. O diagn?stico definitivo ? raramente poss?vel atrav?s da visualiza??o da larva no liquor. Testes sorol?gicos envolvendo principalmente a detec??o do componente de 31-kDa, que apresenta alta sensibilidade e especificidade, tem sido empregados como uma alternativa diagn?stica. O ant?geno de 31-kDa ? composto por glicoprote?nas e tentativas em produzi-lo de maneira recombinante, em modelos procari?ticos ou eucari?ticos, n?o tiveram sucesso em manter o reconhecimento imunol?gico, provavelmente pela incorreta ou insuficiente glicosila??o das mol?culas. Devido ? falta de informa??o sobre glic?dios de A. cantonensis e a necessidade de produzir um ant?geno padr?o capaz de ser usado em um teste de diagn?stico para distribui??o mundial, o objetivo principal deste trabalho foi estudar o perfil glic?dico de A. cantonensis e o papel dessas mol?culas no imunodiagn?stico da ME. Foram utilizados extratos sol?veis totais (ET) de vermes adultos machos e f?meas e produtos de excre??o e secre??o (ES) como fontes dos glic?dios e glicoconjugados estudados. Os N-glic?dios e glicoconjugados do ET de verme f?mea de A. cantonensis e o ant?geno de 31-kDa foram analisados e identificados por espectrometria de massa (EM) e lectina array, e a import?ncia imunog?nica destas mol?culas foi caracterizada por tratamento com glicosidases aliado a dot blot ou Western blot. Al?m disso, foi investigada a presen?a de enzimas envolvidas na s?ntese de diferentes glic?dios a partir de an?lises in silico do genoma e transcriptoma do Angiostrongylus. Diversos N-glic?dios foram identificados nas amostras analisadas. Estes continham estruturas complexas, com antenas truncadas contendo galactose e N-acetilgalactosamina em posi??es terminais e n?cleo ?1-6 fucosilado. As mesmas estruturas terminais foram identificadas pela an?lise de Lectin Array. Em rela??o a an?lise in s?lico da bioss?ntese de glic?dios em Angiostrongylus, foram identificados oito genes envolvidos na s?ntese de N-glic?dios, entre eles GCS1; GANAB, MAN1, MGAT2 e FUT8; e pelo menos tr?s genes envolvidos na s?ntese de O-glic?dios, GALNT, C1GALT1 e OFUT1. Os N-glic?dios se mostraram essenciais para a imunogenicidade do ant?geno de 31-kDa quando soros positivos para A. cantonensis foram testados. Uma modelagem in silico dos componentes de 31-kDa, demonstrou s?tios de glicosila??o para N-glic?dios, e previu as mesmas estruturas que foram identificadas por EM. Em conjunto, os dados gerados neste estudo mostraram a import?ncia de glic?dios para o diagn?stico de angiostrongil?ases e tamb?m o repert?rio de glic?dios que este parasito pode produzir. Este trabalho ? uma contribui??o importante para o desenvolvimento de um diagn?stico padr?o para a ME e tamb?m para novas perspectivas no estudo do diagn?stico de angiostrongil?ases, biologia do parasito e rela??o parasito-hospedeiro.
17
Guerino, Laura Rocha [UNESP]. "Ocorrência de Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) em Achatina fulica (Mollusca, Gastropoda) na Baixada Santista." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/124068.
Full textAbstract:
Made available in DSpace on 2015-06-17T19:34:44Z (GMT). No. of bitstreams: 0
Previous issue date: 2014-02-26. Added 1 bitstream(s) on 2015-06-18T12:47:09Z : No. of bitstreams: 1
000828361.pdf: 2321021 bytes, checksum: 942618e7aef880679b1a5900df78de46 (MD5)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Angiostrongylus cantonensis é o agente etiológico da meningoencefalite eosinofílica, também chamado de verme do pulmão do rato. Mundialmente, a expansão desse nematóide está ligada à dispersão e colonização pelo caracol gigante africano (Achatina fulica). O objetivo deste trabalho foi verificar a ocorrência de larvas de A. cantonensis em moluscos A. fulica naturalmente infectados, nos nove municípios que compõem a Baixada Santista. Foram capturados 540 exemplares na área urbana dos municípios: São Vicente, Santos, Praia Grande, Cubatão, Mongaguá, Itanhaém, Peruíbe, Guarujá e Bertioga. A técnica utilizada para a obtenção de larvas foi a de Wallace & Rosen e sedimentação, por 3 h, pelo método de Baermann. Após a sedimentação, o material foi observado em microscópio estereoscópico de campo claro, para visualização das larvas que foram posteriormente congeladas a -70°C. O DNA foi extraído e submetido à PCR-RFLP (reação em cadeia da polimerase e polimorfismo de tamanho de fragmentos de restrição) direcionada para a região espaçadora transcrita interna dois (ITS2-rDNA) e clivada com a enzima ClaI. Para as análises morfológicas e morfométricas foram utilizadas 60 larvas, sendo 30 em estágio L2 e 30 em L3. No total, foram analisados 90 pontos (bairros), dos quais 73 (81,11%) apresentaram moluscos com larvas de nematóides. Dos 540 caracóis analisados, 204 (37,77%) apresentaram larvas de nematóides, sendo que 79 (38,72%) foram submetidos às análises moleculares; constatou-se a infecção por A. cantonensis em 63 (79,75%) moluscos. O número de larvas por molusco variou de 1 a 9.723 em exemplares que apresentavam 61,0 e 70,0 mm de comprimento de concha, respectivamente. Não foram observadas diferenças significativas entre o número de larvas de A. cantonensis e o comprimento das conchas dos indivíduos adultos jovens e adultos e nem entre as médias de larvas encontradas entre indivíduos ...
Angiostrongylus cantonensis is the causative agent of eosinophilic meningoencephalitis, also called rat lungworm. Worldwide, the expansion of this nematode is linked to the dispersal and colonization by giant African snail (Achatina fulica). The aim of this work was to verify the occurrence of larvae of A. cantonensis in mollusks A. fulica naturally infected in nine cities that compose the Baixada Santista region. About 540 specimens were collected in urban areas of the following cities: São Vicente, Santos, Praia Grande, Cubatão, Mongaguá, Itanhaém, Peruibe, Guarujá and Bertioga. The technique used for obtaining larvae was based on Wallace & Rosen and sedimentation for 3 h using the method of Baermann. After sedimentation, the material was observed under bright-field stereoscopic microscope for viewing larvae that were subsequently frozen at -70°C. DNA was extracted and submitted to PCR - RFLP (polymerase chain reaction and restriction fragment length polymorphism) directed to the internal transcribed spacer region two (ITS2 - rDNA) and cleaved with ClaI enzyme. For morphological and morphometric analyses, 60 larvae were used, 30 in stage L2 and 30 in stage L3. Overall, 90 points (neighborhoods) were analyzed, of which 73 (81.11 %) showed mollusks with nematode larvae. Of the 540 snails examined, 204 (37.77 %) showed nematode larvae, and 79 (38.72 %) were submitted to molecular analyses, and infection by A. cantonensis was found in 63 (79.75 %) mollusks. The number of larvae per mollusk varied from 1 to 9723 in specimens showing 61.0 and 70.0 mm in shell length, respectively. No significant differences were observed between number of larvae of A. cantonensis and the shell length of young adults and adults, nor between the average number of larvae found between young adults and adults. Significant differences were found when comparing the number of young adult and adult individuals ...
18
Guerino, Laura Rocha. "Ocorrência de Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) em Achatina fulica (Mollusca, Gastropoda) na Baixada Santista /." Botucatu, 2014. http://hdl.handle.net/11449/124068.
Full textAbstract:
Orientador: Reinaldo José da SilvaCoorientador: Iracy Lea Pecora
Banca: Pedro Luiz Silva Pinto
Banca: Marlene Tiduko Ueta
Banca: Maria Isabel Muller
Banca: Fábio Hideki Yamada
Resumo: Angiostrongylus cantonensis é o agente etiológico da meningoencefalite eosinofílica, também chamado de "verme do pulmão do rato". Mundialmente, a expansão desse nematóide está ligada à dispersão e colonização pelo caracol gigante africano (Achatina fulica). O objetivo deste trabalho foi verificar a ocorrência de larvas de A. cantonensis em moluscos A. fulica naturalmente infectados, nos nove municípios que compõem a Baixada Santista. Foram capturados 540 exemplares na área urbana dos municípios: São Vicente, Santos, Praia Grande, Cubatão, Mongaguá, Itanhaém, Peruíbe, Guarujá e Bertioga. A técnica utilizada para a obtenção de larvas foi a de Wallace & Rosen e sedimentação, por 3 h, pelo método de Baermann. Após a sedimentação, o material foi observado em microscópio estereoscópico de campo claro, para visualização das larvas que foram posteriormente congeladas a -70°C. O DNA foi extraído e submetido à PCR-RFLP (reação em cadeia da polimerase e polimorfismo de tamanho de fragmentos de restrição) direcionada para a região espaçadora transcrita interna dois (ITS2-rDNA) e clivada com a enzima ClaI. Para as análises morfológicas e morfométricas foram utilizadas 60 larvas, sendo 30 em estágio L2 e 30 em L3. No total, foram analisados 90 pontos (bairros), dos quais 73 (81,11%) apresentaram moluscos com larvas de nematóides. Dos 540 caracóis analisados, 204 (37,77%) apresentaram larvas de nematóides, sendo que 79 (38,72%) foram submetidos às análises moleculares; constatou-se a infecção por A. cantonensis em 63 (79,75%) moluscos. O número de larvas por molusco variou de 1 a 9.723 em exemplares que apresentavam 61,0 e 70,0 mm de comprimento de concha, respectivamente. Não foram observadas diferenças significativas entre o número de larvas de A. cantonensis e o comprimento das conchas dos indivíduos adultos jovens e adultos e nem entre as médias de larvas encontradas entre indivíduos ...
Abstract: Angiostrongylus cantonensis is the causative agent of eosinophilic meningoencephalitis, also called "rat lungworm". Worldwide, the expansion of this nematode is linked to the dispersal and colonization by giant African snail (Achatina fulica). The aim of this work was to verify the occurrence of larvae of A. cantonensis in mollusks A. fulica naturally infected in nine cities that compose the "Baixada Santista" region. About 540 specimens were collected in urban areas of the following cities: São Vicente, Santos, Praia Grande, Cubatão, Mongaguá, Itanhaém, Peruibe, Guarujá and Bertioga. The technique used for obtaining larvae was based on Wallace & Rosen and sedimentation for 3 h using the method of Baermann. After sedimentation, the material was observed under bright-field stereoscopic microscope for viewing larvae that were subsequently frozen at -70°C. DNA was extracted and submitted to PCR - RFLP (polymerase chain reaction and restriction fragment length polymorphism) directed to the internal transcribed spacer region two (ITS2 - rDNA) and cleaved with ClaI enzyme. For morphological and morphometric analyses, 60 larvae were used, 30 in stage L2 and 30 in stage L3. Overall, 90 points (neighborhoods) were analyzed, of which 73 (81.11 %) showed mollusks with nematode larvae. Of the 540 snails examined, 204 (37.77 %) showed nematode larvae, and 79 (38.72 %) were submitted to molecular analyses, and infection by A. cantonensis was found in 63 (79.75 %) mollusks. The number of larvae per mollusk varied from 1 to 9723 in specimens showing 61.0 and 70.0 mm in shell length, respectively. No significant differences were observed between number of larvae of A. cantonensis and the shell length of young adults and adults, nor between the average number of larvae found between young adults and adults. Significant differences were found when comparing the number of young adult and adult individuals ...
Doutor
19
Os?rio, Joana Borges. "Intera??o entre Angiostrongylus cantonensis e Angiostrongylus costaricensis (Nematoda; Metastrongyloidea) com moluscos hospedeiros intermedi?rios e pesquisa de biomarcadores de infec??o." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2017. http://tede2.pucrs.br/tede2/handle/tede/7778.
Full textAbstract:
Submitted by PPG Zoologia (zoologia-pg@pucrs.br) on 2017-12-15T11:05:20Z
No. of bitstreams: 1
Versao Final Tese Oso?rio, Joana.pdf: 10763697 bytes, checksum: 9f6c3be8f4dc2a159d6a919f99d8d23f (MD5)Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-12-19T19:10:24Z (GMT) No. of bitstreams: 1 Versao Final Tese Oso?rio, Joana.pdf: 10763697 bytes, checksum: 9f6c3be8f4dc2a159d6a919f99d8d23f (MD5)
Made available in DSpace on 2017-12-19T19:16:58Z (GMT). No. of bitstreams: 1 Versao Final Tese Oso?rio, Joana.pdf: 10763697 bytes, checksum: 9f6c3be8f4dc2a159d6a919f99d8d23f (MD5) Previous issue date: 2017-08-18
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
Angiostrongylus cantonensis and A. costaricensis can accidentally infect humans causing eosinophilic meningitis and abdominal angiostrongyliasis, respectively. Both species have several mollusks as intermediate hosts. Diagnosing the infection requires killing the mollusks, interfering in conservational and population dynamics studies. This work had the objective to demonstrate possible biological markers in infected intermediate hosts, as well as to investigate specific factors related to the parasite-host relationship of mollusks and Angiostrongylus parasites. Mollusks of the Veronicellidae family were infected with A. cantonensis L1. Mucus and feces from these infected and uninfected animals were used for differential expression analysis of proteins using mass spectrometry and the microbiome profile analysis was performed through the 16S gene sequencing, respectively. LC-MS/MS spectrometry analysis showed an increase in F-BAR proteins subfamily and a decrease in the elongation factor of Mycoplasma spp. Microbiome of feces from infected slugs presented a decrease of the Bacteroidetes phylum. We have also analyzed the microbiome profile of Biomphalaria glabrata feces infected with A. cantonensis and Schistosoma mansoni. We could observe a decrease of Vogesella genus. When infected with S. mansoni, a reduction of Mycoplasma and Nitrospira genera and an increase in Niabella genus could be demonstrated. In both infections, decrease of Fluviicola genus and increase of organisms of the Weeksellaceae family were significant. Our results showed that proteins and microorganisms could be promising biomarkers of A. cantonensis infection in intermediate hosts in vivo. During the collection of the mollusks, the invasive species Meghimatium pictum infected with A. costaricensis was found, associated to a case of abdominal angiostrongyliasis, reported in this thesis. Parallel to this study, the postures of Limax sp. and Phyllocaulis sp. were registered to investigate the embryogenesis of these animals. In postures and in decomposing mollusks, mites of the species Caloglyphus berlesei were also identified and this association was observed for the first time.
Angiostrongylus cantonensis e A. costaricensis podem infectar o ser humano acidentalmente causando a meningite eosinof?lica e a angiostrongil?ase abdominal, respectivamente. Ambos possuem diversas esp?cies de moluscos como hospedeiros intermedi?rios. O diagn?stico da infec??o requer a morte destes moluscos, interferindo em estudos de conserva??o e din?mica populacional. Este trabalho teve como objetivo principal encontrar poss?veis marcadores biol?gicos para a infec??o de hospedeiros intermedi?rios, bem como investigar fatores relacionados a rela??o parasito-hospedeiro entre moluscos e Angiostrongylus cantonensis. Exemplares de Phyllocaulis sp. foram infectados com larva de primeiro est?dio de A. cantonensis. O muco e fezes destes animais infectados e n?o infectados foram utilizados para an?lise de express?o diferencial de prote?nas por espectrometria de massas e an?lise do perfil do microbioma por sequenciamento do gene ribossomal 16S, respectivamente. As an?lises de espectrometria por LC-MS/MS mostraram um aumento das prote?nas da subfam?lia F-BAR e diminui??o do fator de alongamento de Mycoplasma spp. O microbioma das fezes de lesmas infectadas apresentou uma diminui??o do filo Bacteroidetes. Tamb?m foram analisados o perfil do microbioma de fezes de Biomphalaria glabrata infectadas com A. cantonensis e Schistosoma mansoni. Nas fezes de caramujos infectados com A. cantonensis foram observados uma diminui??o do g?nero Vogesella; J? quando infectados com S. mansoni, houve redu??o dos g?neros Mycoplasma e Nitrospira e aumento de Niabella. Em ambas as infec??es, a diminui??o de Fluviicola e o aumento de organismos da fam?lia Weeksellaceae foram significativos. Estes resultados mostram que prote?nas e microorganismos s?o promissores biomarcadores da infec??o de A. cantonensis em hospedeiros intermedi?rios, objetivando o desenvolvimento de m?todos diagn?sticos in vivo. Durante as coletas de moluscos, foi encontrada, pela primeira vez, a esp?cie invasora Meghimatium pictum infectada com A. costaricensis associada a um caso de Angiostrongil?ase abdominal, relato que comp?e essa tese. Paralelamente a este estudo, foram acompanhadas as posturas de Limax sp. e Phyllocaulis sp. durante o desenvolvimento embrion?rio, com o objetivo de registrar e investigar a embriog?nese destes hospedeiros. Nas posturas e em moluscos em decomposi??o, foram identificados ?caros da esp?cie Caloglyphus berlesei, em associa??o observada pela primeira vez.
20
Rosa, Thaise Paím da. "Estudo das proteínas HSP70, SOD e catalase de Angiostrongylus cantonensis em hospedeiros habituais e acidentais." Pontifícia Universidade Católica do Rio Grande do Sul, 2014. http://hdl.handle.net/10923/6695.
Full textAbstract:
Made available in DSpace on 2014-07-12T02:02:26Z (GMT). No. of bitstreams: 1
000459196-Texto+Completo-0.pdf: 1894686 bytes, checksum: 241ac90cbaf1917d1bf7a9c7970d7bb6 (MD5)
Previous issue date: 2014Angiostrongylus cantonensis is a nematode of the Angiostrongylidae family. In humans, they can cause eosinophilic meningitis. Although the parasite have been described in 1935, little is known about the mechanisms of adaptation to its hosts. A. cantonensis owns molecules that help the parasite in the evasion of the host´s immune system, among these are SOD, catalase and HSP70. The main goal of this work was to study the profiles of HSP70, SOD and CAT of L5 produced in different hosts and analyze the diagnostic potential of the extracts. For the obtainment of fifth stage larvae (L5), rats and mice were infeceted with 104 and 50 third stage larvae, respectively, and euthanized after 21 days. The L5 were recovered from the brain of the animals and homogenized in different buffers to obtain the protein extracts. The quantification of the total protein suggests no significant difference between extracts. For the identification of HSP70, it was performed dot blot and Western blot, showing that in both samples HSP70 were present. ELISA assays were used for the quantification of HSP70 and analyze antigen-antibody binding with from individuals with confirmed diagnosis for angiostrongyliasis. L5 from usual hosts were recognized by the sera showing superior titles to the L5 from the accidental hosts. Levels of stress proteins HSP70, SOD (339U versus 0. 08U of SOD/mg of protein) and catalase (184. 4 versus 75. 6 pmol of CAT mg-1 proteins) were higher p<0. 05, in A. cantonensis larvae from usual hosts in comparison to the larvae obtained from accidental hosts. These data suggest that the levels of stress proteins may be associated to the adaptation of A. cantonensis to the usual hosts.
Angiostrongylus cantonensis é um nematódeo da família Angiostrongylidae. Em humanos, podem causar meningite eosinofílica. Apesar de o parasito ter sido descrito em 1935, pouco se sabe dos mecanismos de adaptação aos seus hospedeiros. A. cantonensis possui moléculas que o auxilia na evasão do sistema imune do hospedeiro, dentre estas estão SOD, catalase e HSP70. O objetivo principal deste trabalho foi estudar o perfil de HSPs 70, SOD e CAT de L5 produzidas em diferentes hospedeiros e analisar o potencial diagnóstico dos extratos. Para a obtenção das larvas de quinto estágio (L5), ratos e camundongos foram infectados com 104 e 50 larvas de terceiro estágio, respectivamente, e eutanasiados após 21 dias. As L5 foram recuperadas do cérebro dos animais e homogeneizadas em diferentes tampões para obtenção dos extratos protéicos.A quantificação de proteínas totais sugere não haver diferenças significativas entre os extratos. Para a identificação de HSP70 foram realizados dot blot e Western blot, mostrando que em ambas as amostras HSP70 estavam presentes. Ensaios de ELISA foram utilizados para a quantificação de HPS70 e para analisar a ligação antígeno anticorpo com soros de indíviduos com diagnóstico confirmado para a angiostrongiliase, sendo que L5 provenientes de hospedeiros habituais apresentam reconhecimento pelos soros com títulos superiores às L5 provenientes de hospedeiros habituais. Os níveis das proteínas de estresse HSP70, SOD (339U versus 0,08U de SOD/mg de proteína) e catalase (184,4 versus 75,6 pmole de CAT mg-1 proteínas) foram superiores p<0,05, em larvas de A. cantonensis provenientes de hospedeiros habituais em comparação com as larvas obtidas de hospedeiros acidentais. Estes dados sugerem que os níveis de proteínas de estresse podem estar associados a adaptação do A. cantonensis aos hospedeiros habituais.
21
Perich, Brad C. "Angiostrongylus cantonensis| Epidemiologic Review, Location-Specific Habitat Modelling, and Surveillance in Hillsborough County, Florida, U.S.A." Thesis, University of South Florida, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10748579.
Full textAbstract:
Angiostrongylus cantonensis is a parasitic nematode endemic to tropical and subtropical regions and is the leading cause of human eosinophilic meningitis. The parasite is commonly known as rat lungworm because the primary host in its lifecycle is the rat. A clinical overview of rat lungworm infection is presented, followed by a literature review of rat lungworm epidemiology, risk factors, and surveillance projects. Data collected from previous snail surveys in Florida was considered alongside elevation, population per square kilometer, median household income by zip code territory, and normalized difference vegetation index specific to the geographic coordinates from which the snail samples were retrieved. The parameters of interest were incorporated as possible predictor variables in a Poisson probability regression model and a negative binomial regression model. NDVI and population density were determined to be positively associated with number of snail samples positive for A. cantonensis in a given Miami-based location. A surveillance project was conducted in Hillsborough County, Florida, U.S.A.. Snail samples were collected and tested for A. cantonensis DNA via polymerase chain reaction (PCR) and gel electrophoresis. None of the samples tested positive for A. cantonensis.
22
Perich, Brad Christian. "Angiostrongylus cantonensis: Epidemiologic Review, Location-Specific Habitat Modelling, and Surveillance in Hillsborough County, Florida, U.S.A." Scholar Commons, 2018. https://scholarcommons.usf.edu/etd/7216.
Full textAbstract:
Angiostrongylus cantonensis is a parasitic nematode endemic to tropical and subtropical regions and is the leading cause of human eosinophilic meningitis. The parasite is commonly known as rat lungworm because the primary host in its lifecycle is the rat. A clinical overview of rat lungworm infection is presented, followed by a literature review of rat lungworm epidemiology, risk factors, and surveillance projects. Data collected from previous snail surveys in Florida was considered alongside elevation, population per square kilometer, median household income by zip code territory, and normalized difference vegetation index specific to the geographic coordinates from which the snail samples were retrieved. The parameters of interest were incorporated as possible predictor variables in a Poisson probability regression model and a negative binomial regression model. NDVI and population density were determined to be positively associated with number of snail samples positive for A. cantonensis in a given Miami-based location. A surveillance project was conducted in Hillsborough County, Florida, U.S.A.. Snail samples were collected and tested for A. cantonensis DNA via polymerase chain reaction (PCR) and gel electrophoresis. None of the samples tested positive for A. cantonensis.
23
Rosa, Thaise Pa?m da. "Estudo das prote?nas HSP70, SOD e catalase de Angiostrongylus cantonensis em hospedeiros habituais e acidentais." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2014. http://tede2.pucrs.br/tede2/handle/tede/275.
Full textAbstract:
Made available in DSpace on 2015-04-14T13:09:47Z (GMT). No. of bitstreams: 1
459196.pdf: 1894686 bytes, checksum: 241ac90cbaf1917d1bf7a9c7970d7bb6 (MD5)
Previous issue date: 2014-03-14Angiostrongylus cantonensis is a nematode of the Angiostrongylidae family. In humans, they can cause eosinophilic meningitis. Although the parasite have been described in 1935, little is known about the mechanisms of adaptation to its hosts. A. cantonensis owns molecules that help the parasite in the evasion of the host?s immune system, among these are SOD, catalase and HSP70. The main goal of this work was to study the profiles of HSP70, SOD and CAT of L5 produced in different hosts and analyze the diagnostic potential of the extracts. For the obtainment of fifth stage larvae (L5), rats and mice were infeceted with 104 and 50 third stage larvae, respectively, and euthanized after 21 days. The L5 were recovered from the brain of the animals and homogenized in different buffers to obtain the protein extracts. The quantification of the total protein suggests no significant difference between extracts. For the identification of HSP70, it was performed dot blot and Western blot, showing that in both samples HSP70 were present. ELISA assays were used for the quantification of HSP70 and analyze antigen-antibody binding with from individuals with confirmed diagnosis for angiostrongyliasis. L5 from usual hosts were recognized by the sera showing superior titles to the L5 from the accidental hosts. Levels of stress proteins HSP70, SOD (339U versus 0.08U of SOD/mg of protein) and catalase (184.4 versus 75.6 pmol of CAT mg-1 proteins) were higher p<0.05, in A. cantonensis larvae from usual hosts in comparison to the larvae obtained from accidental hosts. These data suggest that the levels of stress proteins may be associated to the adaptation of A. cantonensis to the usual hosts.
Angiostrongylus cantonensis ? um nemat?deo da fam?lia Angiostrongylidae. Em humanos, podem causar meningite eosinof?lica. Apesar de o parasito ter sido descrito em 1935, pouco se sabe dos mecanismos de adapta??o aos seus hospedeiros. A. cantonensis possui mol?culas que o auxilia na evas?o do sistema imune do hospedeiro, dentre estas est?o SOD, catalase e HSP70. O objetivo principal deste trabalho foi estudar o perfil de HSPs 70, SOD e CAT de L5 produzidas em diferentes hospedeiros e analisar o potencial diagn?stico dos extratos. Para a obten??o das larvas de quinto est?gio (L5), ratos e camundongos foram infectados com 104 e 50 larvas de terceiro est?gio, respectivamente, e eutanasiados ap?s 21 dias. As L5 foram recuperadas do c?rebro dos animais e homogeneizadas em diferentes tamp?es para obten??o dos extratos prot?icos. A quantifica??o de prote?nas totais sugere n?o haver diferen?as significativas entre os extratos. Para a identifica??o de HSP70 foram realizados dot blot e Western blot, mostrando que em ambas as amostras HSP70 estavam presentes. Ensaios de ELISA foram utilizados para a quantifica??o de HPS70 e para analisar a liga??o ant?geno anticorpo com soros de ind?viduos com diagn?stico confirmado para a angiostrongiliase, sendo que L5 provenientes de hospedeiros habituais apresentam reconhecimento pelos soros com t?tulos superiores ?s L5 provenientes de hospedeiros habituais. Os n?veis das prote?nas de estresse HSP70, SOD (339U versus 0,08U de SOD/mg de prote?na) e catalase (184,4 versus 75,6 pmole de CAT mg-1 prote?nas) foram superiores p<0,05, em larvas de A. cantonensis provenientes de hospedeiros habituais em compara??o com as larvas obtidas de hospedeiros acidentais. Estes dados sugerem que os n?veis de prote?nas de estresse podem estar associados a adapta??o do A. cantonensis aos hospedeiros habituais.
24
Osório, Joana Borges. "Estudo sobre a inibição da oviposição em Angiostrongylus cantonensis mediada por agonista e antagonista da serotonina." Pontifícia Universidade Católica do Rio Grande do Sul, 2012. http://hdl.handle.net/10923/5314.
Full textAbstract:
Made available in DSpace on 2013-08-07T19:12:03Z (GMT). No. of bitstreams: 1
000440746-Texto+Completo-0.pdf: 8186310 bytes, checksum: 2ddc0fe8a6260707c7754fc8b9f27f5b (MD5)
Previous issue date: 2012The Angiostrongylus genus includes two species that can infect humans, A. cantonensis and A. costaricensis. They may cause infections known as eosinophilic meningitis and abdominal angiostrongyliasis, respectively. In A. costaricensis infection, eggs and larvae are central elements in the inflammatory reactions, which may get worse with death of the worms. The currently available anthelmintics act on the parasite essential metabolic pathways with a killing effect. Therefore, an alternative substance to treat angiostrongyliasis, acting mainly in worms reproduction is necessary. An in vitro study conducted with Schistosoma mansoni showed inhibition of oviposition by Phenanthroline. In another study with Caenorhabditis elegans, it was shown that serotonin increases the egg-laying rate of the female nematode, besides controlling the change of its posture state (rest and activation). Serotonin is a neurotransmitter present in vertebrates and invertebrates. In order to test the effect on egg laying of Angiostrongylus spp., two substances that interfere with serotonin neurotransmission in humans, Buspirone and Pizotifen, were used in an experimental model in vivo. 28 rodents of species Rattus norvegicus were divided into three groups and infected with 100 L3 of A. cantonensis: a control group (untreated) and two groups treated with each substance. The substances were administered as soon as all rodents started releasing larvae, once a day, orally, for 10 days, at a concentration of 0. 03 mg / mL each. In this period rodent feces were collected daily for counting the number of L1 and after 10 days the animals were euthanized for collection of the worms. The average number of larvae released in feces was 37,934 by the Control group, 10,658 by the Buspirone group and 6,658 by the Pizotifen group. The worms were counted and separated by sex: in the Control group 59 females and 40 males were obtained; in Buspirone group 86 females and 41 males were found; and in the Pizotifen group 83 females and 64 males were counted. The comparison of data from Control and Experimental groups was statistically analyzed by ANOVA and no significant difference was observed. Females were measured using a millimetric eyepiece installed in a stereomicroscope. The ANOVA analysis resulted in a significant difference between Control and Pizotifen, which had an average size of 18 mm, compared with to average size of 19 mm of Control, indicating that Pizotifen would have some effect in the nematodes development, but not affecting their reproduction. These results indicate that the search for alternative drugs that act on egg laying needs a better understanding of the pathways that regulate the reproductive system of parasitic organisms.
O gênero Angiostrongylus engloba duas espécies A. cantonensis e A. costaricensis, que podem infectar o ser humano e causar infecções conhecidas como meningite eosinofílica e angiostrongilíase abdominal, respectivamente. Na infecção por A. costaricensis, ovos e larvas são elementos centrais nas reações inflamatórias podendo haver o agravamento dessas lesões, pela morte dos vermes. Os anti-helmínticos atualmente disponíveis, atuam em vias metabólicas essenciais ao parasito, culminando com a morte dos vermes. Portanto, uma droga alternativa para o tratamento das angiostrongilíases, que atue principalmente na reprodução dos vermes, se torna necessária. Um estudo in vitro realizado com Schistosoma mansoni demonstrou a inibição da oviposição pela fenantrolina. Em outro estudo com Caenorhabditis elegans foi demonstrado que a serotonina estimula o aumento da taxa de ovos liberados pela fêmea do nematódeo, além de controlar a alteração do seu estado de postura (repouso e ativação). A serotonina é um neurotransmissor presente tanto em vertebrados como em invertebrados .Com o objetivo de testar o efeito na oviposição de Angiostrongylus cantonensis, duas substâncias que interferem na neurotransmissão da serotonina em humanos, Buspirona e Pizotifeno, foram utilizadas em modelo experimental in vivo. 28 roedores da espécie Rattus norvegicus foram divididos em 3 grupos e infectados com 100 L3 de A. cantonensis: um grupo controle (não tratado) e 2 grupos tratados com cada substância. As substâncias foram administradas a partir do momento em que todos os roedores iniciaram a larvipostura, uma vez ao dia por via oral, durante 10 dias, numa concentração de 0,03 mg/mL cada. Neste período as fezes dos roedores foram recolhidas diariamente para a contagem do número de L1 eliminadas e, após os 10 dias, os animais foram eutanasiados para coleta dos vermes. A média de larvas eliminadas nas fezes para o grupo controle foi 37. 934, para o grupo Buspirona 10. 658 e para o grupo Pizotifeno 6. 658. Os vermes foram contados e separados pelo sexo: no grupo controle foram obtidas 59 fêmeas e 40 machos; no grupo Buspirona foram encontradas 86 fêmeas e 41 machos e no grupo Pizotifeno 83 fêmeas e 64 machos. A comparação dos dados dos grupos experimentais e do controle foram analisadas estatisticamente pelo teste ANOVA e nenhuma diferença significativa foi verificada. As fêmeas foram medidas através de uma ocular milimetrada instalada em um estereomicroscópio.A análise foi feita também pelo teste ANOVA e resultou numa diferença significativa entre o grupo controle e o Pizotifeno, no qual teve um tamanho médio de 18 mm, em comparação com o tamanho médio do controle de 19 mm, indicando que o Pizotifeno poderia ter algum efeito no desenvolvimento dos nematódeos, porém não afetando a reprodução. Estes resultados indicam que para a procura de drogas alternativas, que atuem na oviposição, é necessário uma melhor compreensão das vias reguladoras do sistema reprodutivo dos organismos parasitos.
25
Oliveira, Camila Krug de. "Expressão diferencial de proteínas durante a maturação sexual de Angiostrongylus cantonensis em infecção experimental de Rattus norvegicus." Pontifícia Universidade Católica do Rio Grande do Sul, 2012. http://hdl.handle.net/10923/1347.
Full textAbstract:
Made available in DSpace on 2013-08-07T18:41:44Z (GMT). No. of bitstreams: 1
000444695-Texto+Completo-0.pdf: 624430 bytes, checksum: e4547549fd33f8db2dfcdef98c9ca071 (MD5)
Previous issue date: 2012The two parasites in the genus Angionstrongylus that cause disease in humans are Angiostrongylus costarisensis and Angiostrongylus cantonensis. They have different target organs: A. costarisensis is located in the mesentery and causes eosinophilic ileocolitis, whereas A. cantonensis is a neurotropic parasite responsible for eosinophilic meningoencephalitis. Based on several indications that the sexual maturation is associated with increased pathogenesis in angiostrongylid worms, proteomic analysis was performed on A. cantonensis protein samples, to describe differential protein expression between 21 and 42 days post infection. Triplicated bidimensional electrophoresis was submitted to analysis and 11 proteins were found to be exclusively expressed after sexual maturation. Acetate kinase was the only protein that could be identified after mass spectrometry (LC-MS/MS). Since acetate is an important end-product of the energy metabolism among many parasites but not among their mammalian hosts, acetate formation is an attractive target for the development of new anti-parasitic drugs. Furthermore, studying the adaptations in parasite metabolisms can result in an increased understanding of the host-parasite interaction. This data open opportunities for control interventions and new strategies for molecular diagnosis.
Os principais parasitos do gênero Angionstrongylus que causam doença nos seres humanos são Angiostrongylus costarisensis e Angiostrongylus cantonensis. Eles têm distintos órgãos alvos: A. costarisensis está localizado no mesentério e causa ileocolite eosinofílica, enquanto que A. cantonensis é um parasita neurotrópico responsável pela meningoencefalite eosinofílica. Com base em várias evidências de que a maturação sexual está associada ao aumento da patogênese em vermes deste gênero, uma análise proteômica foi realizada em vermes de A. cantonensis para descrever a expressão diferencial de proteínas entre 21 e 42 dias após a infecção. Eletroforese bidimensional em triplicata foi submetida à análise e 11 proteínas foram encontradas exclusivamente expressas após a maturação sexual. A acetato quinase foi a única proteína identificada por espectrometria de massa (LC-MS/MS). O acetato é um importante produto final do metabolismo energético de muitos parasitos, mas não de seus hospedeiros mamíferos. Sendo assim, é um alvo atrativo para o desenvolvimento de novas drogas anti-parasitárias. Além do mais, o estudo das adaptações no metabolismo do parasito pode resultar em uma maior compreensão da relação parasito-hospedeiro. Este trabalho abre oportunidades para intervenções de controle e novas estratégias de diagnóstico molecular.
26
Silva, Letícia Fonseca da. "Exploração de sistemas in vitro para desenvolvimento larval de Angiostrongylus costaricensis e de A. cantonensis (Nematelminthes, Angiostrongylidae)." Pontifícia Universidade Católica do Rio Grande do Sul, 2010. http://hdl.handle.net/10923/5388.
Full textAbstract:
Made available in DSpace on 2013-08-07T19:12:51Z (GMT). No. of bitstreams: 1
000427622-Texto+Completo-0.pdf: 4680280 bytes, checksum: 75e54492eea58e6d4b33bd97da9115de (MD5)
Previous issue date: 2010Among the angiostrongylid worms there are two extensively studied species because of their importance as causes of human health problems, Angiostrongylus costaricensis and A. cantonensis. While there are numerous in vitro studies on maintenance and evolution of trematodes, the literature is short of reports on similar trials with nematodes. Through cultivation and cocultivation with Biomphalaria glabrata embryonic cells, the best conditions were searched for the production of intra-mollusk larval stages from both parasites under study. A previous reported protocol for axenization of first stage larvae was adjusted especially concerning the concentration of sodium hypochlorite and a 0,25 % preparation was found effective. It was demonstrated that cultivation enriched with crude body proteic extract of B. glabrata resulted in a higher recovery of second stage larvae. In order to investigate the possibility of a differential expression of proteins after infection, bidimensional electrophoresis was performed. A 37 kDa protein with isoeletric point 4,2 was expressed exclusively at tissues of the infected snails. In order to guarantee the supply of first stage larvae for a future in vitro production of L3, several conditions for cryopreservation were assayed and the best larvae yield was achieved with a 1% dimethil-sulphoxide and 5% bovine fetal serum, and incubation time of 60 minutes before quick introduction into liquid nitrogen. In conclusion, although the unsuccessful trial to produce in vitro large amounts of L3, several aspects were assayed and the resulting knowledge may pave the way for the ongoing effort to establish an in vitro system that enables regular large production of parasitic forms.The results now reported have contributed to a better understanding of the complex task of in vitro manipulation of nematodes and the challenges standing ahead for the better understanding of their biology.
Dentre os angiostrongilídeos, há duas espécies que têm sido extensamente estudadas por constituírem problemas de saúde humana: Angiostrongylus costaricensis e A. cantonensis. Enquanto existem numerosos estudos sobre a manutenção e desenvolvimento in vitro de trematódeos, são poucas as tentativas registradas na literatura do mesmo esforço em relação aos nematódeos. Através do desenvolvimento de cultivos e co-cultivos com células embrionárias de B. glabrata buscou-se encontrar a forma mais adequada para a produção de larvas nas fases intramolusco dos dois parasitos em estudo. Em um trabalho anterior foi utilizada a axenização das larvas de primeiro estágio, este método foi adaptado especialmente quanto à concentração de hipoclorito de sódio que foi utilizado a 0,25% de modo eficiente. Verificou-se que nos cultivos enriquecidos com extrato protéico do corpo de B. glabrata, houve uma maior recuperação de larvas de segundo estágio. A fim de se investigar a possibilidade de uma diferença quanto à expressão de proteínas entre caramujos infectados e não infectados, foi realizada a eletroforese bidimensional. Uma proteína de 37 kDa no ponto isoelétrico de 4,2 foi expressa apenas nos tecidos dos caramujos infectados. Para se ter um estoque de larvas de primeiro estágio, testou-se formas de criopreservá-las com diferentes concentrações de dimetil-sulfóxido (DMSO) e de soro fetal bovino (SFB). Para ambos nematódeos, o uso de 1% de DMSO em meio enriquecido com 5% de SFB, incubação prévia de 60 minutos com o criopreservante em temperatura ambiente, seguido pelo congelamento rápido em nitrogênio líquido, apresentou os melhores índices de larvas ativas recuperadas.Embora os experimentos não tenham tido êxito para produzir in vitro larvas de terceiro estágio (L3), vários aspectos foram analisados e todo o conhecimento resultante representa o início do caminho para, com esforço contínuo, se estabelecer um sistema in vitro capaz de produzir em grande quantidade e regularmente essas formas parasitárias. Os resultados apresentados contribuem para uma melhor compreensão da complexa tarefa de manipulação in vitro desses nemátodeos e dos desafios para o melhor entendimento de sua biologia.
27
Silva, Let?cia Fonseca da. "Explora??o de sistemas in vitro para desenvolvimento larval de Angiostrongylus costaricensis e de A. cantonensis (Nematelminthes, Angiostrongylidae)." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2010. http://tede2.pucrs.br/tede2/handle/tede/200.
Full textAbstract:
Made available in DSpace on 2015-04-14T13:09:23Z (GMT). No. of bitstreams: 1
427622.pdf: 4680280 bytes, checksum: 75e54492eea58e6d4b33bd97da9115de (MD5)
Previous issue date: 2010-08-25Dentre os angiostrongil?deos, h? duas esp?cies que t?m sido extensamente estudadas por constitu?rem problemas de sa?de humana: Angiostrongylus costaricensis e A. cantonensis. Enquanto existem numerosos estudos sobre a manuten??o e desenvolvimento in vitro de tremat?deos, s?o poucas as tentativas registradas na literatura do mesmo esfor?o em rela??o aos nemat?deos. Atrav?s do desenvolvimento de cultivos e co-cultivos com c?lulas embrion?rias de B. glabrata buscou-se encontrar a forma mais adequada para a produ??o de larvas nas fases intramolusco dos dois parasitos em estudo. Em um trabalho anterior foi utilizada a axeniza??o das larvas de primeiro est?gio, este m?todo foi adaptado especialmente quanto ? concentra??o de hipoclorito de s?dio que foi utilizado a 0,25% de modo eficiente. Verificou-se que nos cultivos enriquecidos com extrato prot?ico do corpo de B. glabrata, houve uma maior recupera??o de larvas de segundo est?gio. A fim de se investigar a possibilidade de uma diferen?a quanto ? express?o de prote?nas entre caramujos infectados e n?o infectados, foi realizada a eletroforese bidimensional. Uma prote?na de 37 kDa no ponto isoel?trico de 4,2 foi expressa apenas nos tecidos dos caramujos infectados. Para se ter um estoque de larvas de primeiro est?gio, testou-se formas de criopreserv?-las com diferentes concentra??es de dimetil-sulf?xido (DMSO) e de soro fetal bovino (SFB). Para ambos nemat?deos, o uso de 1% de DMSO em meio enriquecido com 5% de SFB, incuba??o pr?via de 60 minutos com o criopreservante em temperatura ambiente, seguido pelo congelamento r?pido em nitrog?nio l?quido, apresentou os melhores ?ndices de larvas ativas recuperadas.Embora os experimentos n?o tenham tido ?xito para produzir in vitro larvas de terceiro est?gio (L3), v?rios aspectos foram analisados e todo o conhecimento resultante representa o in?cio do caminho para, com esfor?o cont?nuo, se estabelecer um sistema in vitro capaz de produzir em grande quantidade e regularmente essas formas parasit?rias. Os resultados apresentados contribuem para uma melhor compreens?o da complexa tarefa de manipula??o in vitro desses nem?todeos e dos desafios para o melhor entendimento de sua biologia.
28
Os?rio, Joana Borges. "Estudo sobre a inibi??o da oviposi??o em Angiostrongylus cantonensis mediada por agonista e antagonista da serotonina." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2012. http://tede2.pucrs.br/tede2/handle/tede/239.
Full textAbstract:
Made available in DSpace on 2015-04-14T13:09:35Z (GMT). No. of bitstreams: 1
440746.pdf: 8186310 bytes, checksum: 2ddc0fe8a6260707c7754fc8b9f27f5b (MD5)
Previous issue date: 2012-05-07The Angiostrongylus genus includes two species that can infect humans, A. cantonensis and A. costaricensis. They may cause infections known as eosinophilic meningitis and abdominal angiostrongyliasis, respectively. In A. costaricensis infection, eggs and larvae are central elements in the inflammatory reactions, which may get worse with death of the worms. The currently available anthelmintics act on the parasite essential metabolic pathways with a killing effect. Therefore, an alternative substance to treat angiostrongyliasis, acting mainly in worms reproduction is necessary. An in vitro study conducted with Schistosoma mansoni showed inhibition of oviposition by Phenanthroline. In another study with Caenorhabditis elegans, it was shown that serotonin increases the egg-laying rate of the female nematode, besides controlling the change of its posture state (rest and activation). Serotonin is a neurotransmitter present in vertebrates and invertebrates. In order to test the effect on egg laying of Angiostrongylus spp., two substances that interfere with serotonin neurotransmission in humans, Buspirone and Pizotifen, were used in an experimental model in vivo. 28 rodents of species Rattus norvegicus were divided into three groups and infected with 100 L3 of A. cantonensis: a control group (untreated) and two groups treated with each substance. The substances were administered as soon as all rodents started releasing larvae, once a day, orally, for 10 days, at a concentration of 0.03 mg / mL each. In this period rodent feces were collected daily for counting the number of L1 and after 10 days the animals were euthanized for collection of the worms. The average number of larvae released in feces was 37,934 by the Control group, 10,658 by the Buspirone group and 6,658 by the Pizotifen group. The worms were counted and separated by sex: in the Control group 59 females and 40 males were obtained; in Buspirone group 86 females and 41 males were found; and in the Pizotifen group 83 females and 64 males were counted. The comparison of data from Control and Experimental groups was statistically analyzed by ANOVA and no significant difference was observed. Females were measured using a millimetric eyepiece installed in a stereomicroscope. The ANOVA analysis resulted in a significant difference between Control and Pizotifen, which had an average size of 18 mm, compared with to average size of 19 mm of Control, indicating that Pizotifen would have some effect in the nematodes development, but not affecting their reproduction. These results indicate that the search for alternative drugs that act on egg laying needs a better understanding of the pathways that regulate the reproductive system of parasitic organisms.
O g?nero Angiostrongylus engloba duas esp?cies A. cantonensis e A. costaricensis, que podem infectar o ser humano e causar infec??es conhecidas como meningite eosinof?lica e angiostrongil?ase abdominal, respectivamente. Na infec??o por A. costaricensis, ovos e larvas s?o elementos centrais nas rea??es inflamat?rias podendo haver o agravamento dessas les?es, pela morte dos vermes. Os anti-helm?nticos atualmente dispon?veis, atuam em vias metab?licas essenciais ao parasito, culminando com a morte dos vermes. Portanto, uma droga alternativa para o tratamento das angiostrongil?ases, que atue principalmente na reprodu??o dos vermes, se torna necess?ria. Um estudo in vitro realizado com Schistosoma mansoni demonstrou a inibi??o da oviposi??o pela fenantrolina. Em outro estudo com Caenorhabditis elegans foi demonstrado que a serotonina estimula o aumento da taxa de ovos liberados pela f?mea do nemat?deo, al?m de controlar a altera??o do seu estado de postura (repouso e ativa??o). A serotonina ? um neurotransmissor presente tanto em vertebrados como em invertebrados. Com o objetivo de testar o efeito na oviposi??o de Angiostrongylus cantonensis, duas subst?ncias que interferem na neurotransmiss?o da serotonina em humanos, Buspirona e Pizotifeno, foram utilizadas em modelo experimental in vivo. 28 roedores da esp?cie Rattus norvegicus foram divididos em 3 grupos e infectados com 100 L3 de A. cantonensis: um grupo controle (n?o tratado) e 2 grupos tratados com cada subst?ncia. As subst?ncias foram administradas a partir do momento em que todos os roedores iniciaram a larvipostura, uma vez ao dia por via oral, durante 10 dias, numa concentra??o de 0,03 mg/mL cada. Neste per?odo as fezes dos roedores foram recolhidas diariamente para a contagem do n?mero de L1 eliminadas e, ap?s os 10 dias, os animais foram eutanasiados para coleta dos vermes. A m?dia de larvas eliminadas nas fezes para o grupo controle foi 37.934, para o grupo Buspirona 10.658 e para o grupo Pizotifeno 6.658. Os vermes foram contados e separados pelo sexo: no grupo controle foram obtidas 59 f?meas e 40 machos; no grupo Buspirona foram encontradas 86 f?meas e 41 machos e no grupo Pizotifeno 83 f?meas e 64 machos. A compara??o dos dados dos grupos experimentais e do controle foram analisadas estatisticamente pelo teste ANOVA e nenhuma diferen?a significativa foi verificada. As f?meas foram medidas atrav?s de uma ocular milimetrada instalada em um estereomicrosc?pio. A an?lise foi feita tamb?m pelo teste ANOVA e resultou numa diferen?a significativa entre o grupo controle e o Pizotifeno, no qual teve um tamanho m?dio de 18 mm, em compara??o com o tamanho m?dio do controle de 19 mm, indicando que o Pizotifeno poderia ter algum efeito no desenvolvimento dos nemat?deos, por?m n?o afetando a reprodu??o. Estes resultados indicam que para a procura de drogas alternativas, que atuem na oviposi??o, ? necess?rio uma melhor compreens?o das vias reguladoras do sistema reprodutivo dos organismos parasitos.
29
Cognato, Bianca Barbieri. "Estudos sobre a especificidade de ant?genos e sua aplicabilidade para o imunodiagn?stico das angiostronil?ases." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2017. http://tede2.pucrs.br/tede2/handle/tede/7457.
Full textAbstract:
Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T17:21:25Z
No. of bitstreams: 1
TES_BIANCA_BARBIERI_COGNATO_COMPLETO.pdf: 2150300 bytes, checksum: 6c0ebf29cb36a3ae7a825cf9c0060dc4 (MD5)Made available in DSpace on 2017-06-30T17:21:25Z (GMT). No. of bitstreams: 1 TES_BIANCA_BARBIERI_COGNATO_COMPLETO.pdf: 2150300 bytes, checksum: 6c0ebf29cb36a3ae7a825cf9c0060dc4 (MD5) Previous issue date: 2017-03-21
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq
Two nematode species belonging to the Metastrongyloidea superfamily are capable to produce disease in humans: Angiostrongylus cantonensis and Angiostrongylus costaricensis. Both are rodent parasites and human infection is considered accidental. Eosinophilic meningoencephalitis, caused by A. cantonensis, raises concern due to the expanding number of cases and geographical area of occurrence. Molecular and immunological methods for the diagnosis are crucial, however, after many studies with different antigenic molecules, has a specific and sensitive test to discriminate the angiostrongyliasis to others parasitoses is lacking. Cross-reactivity with other helminthes, which may cause similar symptoms, and eosinophilic meningitis, has been a problem for the satisfactory performance of specificity in serological tests. In order to improve the diagnosis of angiostrongyliasis, a comparative analysis of protein recognition from different extracts from A. cantonensis, Toxocara canis, Schistosoma mansoni and Strongyloides stercoralis against positive serum for Angiostrongylus.spp was performed. Through extraction kit, one-dimensional and two-dimensional electrophoresis, western blot and mass spectrometry, 149 proteins were identified. Among these, 34 were exclusive to A. cantonensis, COI being present only in the A. cantonensis extract, having no similarities with any other parasite compared in NCBI (nr database) and WormBase Database. Additionally, nine proteins were recognized by more than one parasite and extract, being important cross reactivity markers in parasitic infections. With the data obtained in this study, we suggest the use of the follow proteins as cross-reactivity markers: Galectin 1, HSPA-5 and Ifa1. In addition, immunogenic proteins of A. cantonensis ES-7, Lec-5 and 14-3-3, were recombinant expressed in two cell types, CHO and HEK. Their potential diagnostic values were verified by uni and bidimensional electrophoresis, western and dot blot, and N-glycosidase F (PNGase F) treatment using serum from patients infected with A. cantonensis, negative serum for parasites, and positive for other parasites. ES7 protein expressed in HEK and CHO cells and Lec-5 expressed in CHO were recognized only by Angiostrongylus-positive serum and not by negative control and specificity control in 2D and 1D tests, respectively. In the 2D analyzes Lec-5 showed a weak recognition with negative serum. However, the 14-3-3 protein didn?t show any specificity against A. cantonensis serum, since it was recognized by all tested sera. Antigen-antibody recognition was found to be dependent on the glycogenic portions, since when treated with N-glycosidase F (PNGase F), recognition between proteins and serum disappeared. The heterologous expression, using mammalian cells, as well as the identification of shared and/or specific molecules, may represent a promising source of antigens for the diagnosis of eosinophilic meningoencephalitis, and molecular diagnostic tests become necessary.
Duas esp?cies de nemat?deos pertencentes a superfam?lia Metastrongyloidea, s?o capazes de produzir doen?a em humanos: Angiostrongylus cantonensis e Angiostrongylus costaricensis. Ambos s?o parasitas pr?prios de roedores e a infec??o humana ? considerada acidental. A meningite eosinof?lica, causada por A. cantonensis, tem gerado preocupa??o na comunidade cient?fica pela expans?o da ?rea geogr?fica de ocorr?ncia. M?todos moleculares e imunol?gicos para o diagn?stico desta infec??o s?o cruciais para o diagn?stico, entretanto, ap?s muitos estudos com diferentes mol?culas antig?nicas, at? hoje n?o foi desenvolvido um teste de diagn?stico que seja espec?fico e sens?vel o suficiente para discriminar as angiostrongil?ases de outras parasitoses. A reatividade cruzada tem sido o principal problema encontrado nos estudos j? desenvolvidos para este prop?sito. Com o objetivo de aprimorar o diagn?stico das angiostrongil?ases, foi realizado neste estudo an?lise comparativa do reconhecimento de prote?nas de diferentes extratos teciduais de A. cantonensis, Toxocara canis, Schistosoma mansoni e Strongyloides stercoralis contra soro positivo para Angiostrongylus spp Atrav?s de kit de extra??o, eletroforese unidimensional e bidimensional, western blot e espectrometria de massas foram identificadas 149 prote?nas. Dentre estas, 34 foram exclusivas para A. cantonensis, sendo que COI estava presente apenas no extrato de A. cantonensis n?o possuindo similaridades com nenhum outro parasito comparado no NCBI (nr database) e WormBase Database. Estas prote?nas podem ser consideradas promissoras como marcadores de reatividade humoral espec?fica para o parasito. Todavia, outras nove prote?nas foram reconhecidas por mais de um parasito em mais de um extrato testado, sendo importantes marcadores de reatividade cruzada em infec??es parasit?rias. Com os dados obtidos neste estudo, sugerimos como marcadores de reatividade cruzada o uso das prote?nas: Galectin 1, HSPA-5 e Ifa1. Al?m disso, prote?nas imunog?nicas de A. cantonensis ES-7, Lec-5 e 14-3-3, foram expressas de forma recombinante em dois tipos celulares, CHO e HEK, e o potencial uso diagn?stico destas prote?nas foi verificado atrav?s de eletroforese uni e bidimensional, western e dot blot, e tratamento por N-glycosidase F (PNGase F), utilizando soro positivo para Angiostrongylus spp., soro negativo e positivo para outras parasitoses. Prote?na ES-7 expressa em c?lulas HEK e CHO, e Lec-5 expressa em CHO foram reconhecidas apenas pelo soro positivo para Angiostrongylus spp. e n?o pelo soro negativo e controles de especificidade em testes 2D e 1D, respectivamente. J? nas an?lises em 2D, Lec-5 apresentou fraco reconhecimento com soro negativo. J? a prote?na 14-3-3 n?o apresentou nenhuma especificidade pelo soro de A. cantonensis, j? que foi reconhecida por todos os soros testados. O reconhecimento ant?geno-anticorpo se mostrou dependente das por??es glic?dicas, j? que quando tratadas com N-glycosidase F (PNGase F), o reconhecimento das prote?nas pelo soro desapareceu. A express?o heter?loga, utilizando c?lulas de mam?feros, assim como a identifica??o de mol?culas compartilhadas e/ou espec?ficas, podem representar uma promissora fonte de ant?genos para o diagn?stico da meningite eosinof?lica, requerendo aprimorados testes moleculares para seu diagn?stico.
30
Pascoal, Vanessa Fey. "Efeito imunomodulador de diferentes extratos de Angiostrongylus cantonensis no desenvolvimento de resposta pulmonar al?rgica em um modelo murino." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2017. http://tede2.pucrs.br/tede2/handle/tede/7498.
Full textAbstract:
Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T18:01:01Z
No. of bitstreams: 1
DIS_VANESSA_FEY_PASCOAL_PARCIAL.pdf: 876481 bytes, checksum: 0b2728fa712aaf8f7a7a02a0d8066035 (MD5)Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-06-30T18:01:09Z (GMT) No. of bitstreams: 1 DIS_VANESSA_FEY_PASCOAL_PARCIAL.pdf: 876481 bytes, checksum: 0b2728fa712aaf8f7a7a02a0d8066035 (MD5)
Made available in DSpace on 2017-06-30T18:01:17Z (GMT). No. of bitstreams: 1 DIS_VANESSA_FEY_PASCOAL_PARCIAL.pdf: 876481 bytes, checksum: 0b2728fa712aaf8f7a7a02a0d8066035 (MD5) Previous issue date: 2017-03-31
Asthma is a chronic inflammatory disease of the airways, associated with genetic and environmental factors. Exposure to helminths appears to exert a protective effect on some chronic diseases, such as asthma. The objective of this study was to evaluate the immunomodulatory effect of different extracts of Angiostrongylus cantonensis (A. cantonensis) on the development of allergic pulmonary response by ovalbumin (OVA) in mice. The animals were divided into 6 groups: negative control (PBS), positive control (OVA), animals with asthma immunized with total soluble extract of A. cantonensis (Ac/OVA), animals with proteins from the digestive tract (AcD/OVA), animals with proteins from the reproductive tract (AcR /OVA), and animals with proteins from the cuticle (AcC / OVA). At the end of the OVA protocol, the animals were euthanized for blood collection, bronchoalveolar lavage and lung tissue. OVA-specific IgE was analyzed in blood, and cell counts, IL-4, IL-5, IL-10, INF- ? and eotaxin in bronchoalveolar lavage. Histological analysis of the lung was also performed. A significant reduction in the total cell count was observed in the groups that were immunized with the total soluble extract (TE) and with the soluble extract of the systems (SE) of A. cantonensis, compared to the OVA group (p <0.001). We found a significant reduction of eosinophils, macrophages and lymphocytes in the group immunized with TE, as in the SE groups, in relation to the OVA group. However, intraperitoneal immunization with TE and SE did not alter the levels of OVA-specific IgE and cytokines, except for an increase in IL-10 and IFN-? (p <0.01) in the group immunized with TE. In conclusion, the different worm extracts from A. cantonensis, especially TE, when administered early intraperitoneally, result in a modulation of the OVA-induced allergic lung response in mice, with a distinct response of IL-10 and e INF-? in the TE group.
A asma ? uma doen?a inflamat?ria cr?nica das vias a?reas, associada ? fatores gen?ticos e ambientais. A exposi??o a helmintos parece exercer um efeito protetor sobre algumas doen?as cr?nicas, como a asma. O objetivo deste estudo foi avaliar o efeito imunomodulador de diferentes extratos de Angiostrongylus cantonensis (A. cantonensis) no desenvolvimento da resposta pulmonar al?rgica por ovalbumina (OVA) em camundongos. Os animais foram divididos em 6 grupos: controle negativo (PBS), controle positivo (OVA), animais com asma imunizados com extrato total sol?vel de A. cantonensis (Ac/OVA), com prote?nas do aparelho digestivo (AcD/OVA), com prote?nas do aparelho reprodutor (AcR/OVA), e de prote?nas da cut?cula (AcC/OVA). No final do protocolo de OVA, os animais foram eutanasiados para coleta de sangue, lavado broncoalveolar e tecido pulmonar. Foram dosados IgE espec?fica para OVA no sangue, e contagem de c?lulas, IL-4, IL-5, IL-10, INF- ? e eotaxina (no lavado broncoalveolar) e realizada an?lise histol?gica do pulm?o. Foi observada significativa redu??o na contagem total de c?lulas nos grupos que foram imunizados com o extrato total sol?vel (ET) e com o extrato sol?vel dos sistemas (ES) de A. cantonensis, em rela??o ao grupo OVA (p<0,001). Verificamos uma redu??o significativa de eosin?filos, macr?fagos e linf?citos no grupo imunizado com ET, bem como nos grupos com ES, em rela??o ao grupo OVA. No entanto, a imuniza??o por via intraperitoneal com ET e ES n?o promoveu altera??o significativa nos n?veis de IgE espec?fica para OVA e citocinas avaliadas, exceto aumento de IL-10 e IFN- ? (p<0,01) no grupo imunizado com o ET. Concluindo, os diferentes extratos de vermes de A. cantonensis, em especial o ET, quando administrado precocemente por via intraperitoneal, resulta em uma modula??o na resposta pulmonar al?rgica induzida por OVA em camundongos, com uma resposta distinta de IL-10 e INF- ? no grupo ET.
31
Lima, Mariana Gomes. "Angiostrongylus cantonensis (Nematoda): estudo comparativo dos efeitos da infec??o sobre o metabolismo de Biomphalaria straminea e Biomphalaria tenagophila (Mollusca)." Universidade Federal Rural do Rio de Janeiro, 2016. https://tede.ufrrj.br/jspui/handle/jspui/1474.
Full textAbstract:
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-03-22T13:55:27Z
No. of bitstreams: 1
2016 - Mariana Gomes Lima.pdf: 3236185 bytes, checksum: 3517b88022c7943663ae0274085cc96a (MD5)Made available in DSpace on 2017-03-22T13:55:27Z (GMT). No. of bitstreams: 1 2016 - Mariana Gomes Lima.pdf: 3236185 bytes, checksum: 3517b88022c7943663ae0274085cc96a (MD5) Previous issue date: 2016-02-23
Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq
The nematode Angiostrongylus cantonensis has gained notoriety under the public health point of view out of its endemic area in Asia, since in recent years cases of eosinophilic meningoencephalitis have been reported in other continents, such as in the Americas. In Brazil over thirty cases of this zoonotic disease have been recorded and there are reports of the presence of this nematode in 11 states. In its life cycle A. cantonensis has, along their development, need for intermediate and final host, involving predominantly molluscs and rodents, respectively, and different paratenic hosts, humans are accidental hosts. They can also infect a rich variety of terrestrial and aquatic molluscs and in this study, there were used specimens of neotropical planorbids transmitters of schistosomiasis, Biomphalaria straminea and Biomphalaria tenagophila, born and reared in the laboratoryfor experimental infection by A. cantonensis. In order to characterize the resulting physiological profile that host-parasite relationship, the host molluscs were separated into groups of infected and uninfected containing thirty molluscs per group, all made in triplicates. The investigation lasted 21 days, after the pre-patent period molluscs were dissected to collect hemolymph, removal of tissues and use the shell for calcium concentration check. The reproductive biology of both species was observed on the viability of the eggs, as well as (A) Number of eggs/ eggs mass; (B) Number of eggs/mollusc; (C) Number of eggs mass/mollusc and (D) Number of hatched molluscs/mollusc. In the hemolymph there were quantified total protein, glucose, uric acid, urea, the activity of transaminases AST and ALT, lactate dehydrogenase - LDH, and organic acids (oxalic, pyruvic, succinic and lactic). In the cephalopedal mass and digestive gland the glycogen content was measured and in the albumen gland, it was measured the galactogen. The results showed that the snails infected, of both species showed significant reduction in the viability of eggs, 50% for B. straminea and 10% for B. tenagophila. The metabolic status was also strongly affected, with mobilization of glycogen and hemolymph glucose reduction of 68% to B. straminea and 6.48% for B. tenagophila, followed by anincrease of LDH activity of 518.25% to 320.14% B. straminea and to B. tenagophila as well as the concentration of lactic acid, 5,7mM to B. straminea and 0.34 mM for B. tenagophila, and reduction in the concentration of pyruvic and succinic acids. The intense release of gluconeogenic amino acids from protein catabolism reversed the excretory pattern from uricotelic to ureotelic in both B. tenagophila and B. straminea. The tissue injury showed increase of 69% in the concentration of total proteins in the hemolymph of B. straminea and the intense activity of aminotransferases, ALT and AST, an increase of 241.26% and 360%, respectively, compared to the control group. The infection reduced by 51.57% the concentration of total circulating proteins to B. tenagophila, and increased ALT and AST activity at 310% and 280% compared to uninfected snails. The results of this study indicate that the energetic and structural metabolism of both species is strongly affected by the infection, showing the activation of an anaerobic to compensate for the depletion of glicidics stores as well as the change in the excretory pattern depending on the use of substrates protein for the production of ATP. Although studies on clinical, pathological and epidemiological neural angiostrongyliasis have been extensively carried xiii out, data on the metabolic and reproductive profile of snails infected by A. cantonensis are scarce. This pioneering study focused on species of host-parasite relationship certainly contribute to the knowledge of this interaction, as well as to the epidemiology of transmission A. cantonensis in addition to providing new subsidies which can be used in prevention and control of eosinophilic meningitis, zoonosis considered emerging in Brazil.
O nemat?deo Angiostrongylus cantonensis tem ganhado notoriedade sob o ponto de vista da sa?de p?blica fora de sua ?rea end?mica na ?sia, uma vez que nos ?ltimos anos casos de meningoencefal?te eosinof?lica v?m sendo reportados em outros continentes, como nas Am?ricas. No Brasil mais de trinta casos dessa zoonose j? foram registrados e h? relatos da presen?a desse nemat?deo em 11 estados. Em seu ciclo de vida heteroxeno A. cantonensis tem, ao longo do seu desenvolvimento, a necessidade de hospedeiro intermedi?rio e definitivo, envolvendo predominantemente, moluscos e roedores, respectivamente, al?m de v?rios hospedeiros parat?nicos, sendo o homem um hospedeiro acidental. Pode infectar uma rica variedade de moluscos terrestres e aqu?ticos e neste estudo, foram utilizadas para infec??o experimental popula??es nascidas e criadas em laborat?rio de duas esp?cies de planorb?deos neotropicais transmissores da esquistossomose mans?nica, Biomphalaria straminea e Biomphalaria tenagophila. Com a finalidade de caracterizar o perfil fisiol?gico resultante dessa rela??o hospedeiro-parasito, os moluscos hospedeiros foram separados em grupos de infectados e n?o infectados, contendo trinta moluscos por grupo, todos feitos em triplicatas. A investiga??o durou 21 dias, ao fim do per?odo pr?-patente os moluscos foram dissecados para a coleta de hemolinfa, retirada de tecidos e utiliza??o da concha para verifica??o de concentra??o de c?lcio. A oviposi??o dos moluscos de ambas as esp?cies foi observada quanto ? viabilidade dos ovos, assim como (A) N?mero de massas ov?geras/molusco; (B) N?mero de ovos/molusco; (C) N?mero de ovos/massa ov?gera e (D) N?mero de moluscos eclodidos/molusco. Na hemolinfa foram quantificadas as prote?nas totais, glicose, ?cido ?rico, ureia, a atividade das transaminases ALT e AST, lactato desidrogenase - LDH, e os ?cidos org?nicos (ox?lico, pir?vico, succ?nico e l?tico). Na massa cefalopediosa e gl?ndula digestiva, foram mensurados os conte?dos de glicog?nio e na gl?ndula de alb?men, foi mensurado o galactog?nio. Os resultados obtidos mostraram que os moluscos infectados, de ambas as esp?cies, apresentaram redu??o significativa na viabilidade dos ovos, de 50% para B. straminea e de 10% para B. tenagophila. O estado energ?tico tamb?m foi vigorosamente afetado, com mobiliza??o das reservas de glicog?nio, e queda da glicemia de 68% para B. straminea e de 6,48% para B. tenagophila, seguida de uma eleva??o na atividade da LDH de 518,25% para B. straminea e 320,14% para B. tenagophila, bem como na concentra??o do ?cido l?tico, 5,7mM para B. straminea e 0,34 mM para B. tenagophila, e redu??o na concentra??o dos ?cidos pir?vico e succ?nico. A intensa libera??o de amino?cidos gliconeog?nicos a partir do catabolismo proteico inverteu o padr?o excretor de uricot?lico para ureot?lico tanto em B. tenagophila quanto em B. straminea. A les?o tecidual mostrou eleva??o de 69% na concentra??o das prote?nas totais na hemolinfa de B. straminea, bem como a intensa atividade das aminotransferases, AST e ALT, com aumento de 241,26% e 360%, respectivamente, em rela??o ao grupo controle. A infec??o reduziu em 51,57% a concentra??o de prote?nas totais circulantes para B. tenagophila, e elevou a atividade de ALT e AST em 310% e xi 280% em compara??o com os moluscos n?o infectados. Os resultados observados neste estudo indicam que o metabolismo energ?tico e estrutural de ambas as esp?cies foi vigorosamente afetado pela infec??o, mostrando a ativa??o de uma via anaer?bia para compensar a exaust?o das reservas glic?dicas, assim como a mudan?a no padr?o excretor em fun??o da utiliza??o de substratos proteicos para produ??o de ATP. Embora, estudos sobre aspectos cl?nicos, patol?gicos e epidemiol?gicos da angiostrongil?ase neural t?m sido extensivamente realizados, dados acerca do perfil metab?lico e reprodutivo de moluscos infectados por A. cantonensis s?o escassos. Este estudo pioneiro na rela??o parasito-hospedeiro das esp?cies focadas seguramente contribuir? tanto para o conhecimento dessa intera??o, quanto da epidemiologia da transmiss?o de A. cantonensis, al?m de fornecer novos subs?dios que poder?o ser utilizados em medidas de preven??o e controle da meningite eosinof?lica, zoonose considerada emergente no Brasil
32
TUNHOLI-ALVES, Vin?cius Menezes. "Caracteriza??o do perfil mineral, lip?dico e oxidativo de Biomphalaria glabrata (Mollusca, Gastropoda) infectada experimentalmente por Angiostrongylus cantonensis (Nematoda, Metastrongylidae)." Universidade Federal Rural do Rio de Janeiro, 2015. https://tede.ufrrj.br/jspui/handle/jspui/1741.
Full textAbstract:
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-06T18:14:40Z
No. of bitstreams: 1
2015 - Vin?cius Menezes Tunholi Alves.pdf: 1609397 bytes, checksum: 1c31fd547893728f1c9c9165ee10137c (MD5)Made available in DSpace on 2017-06-06T18:14:40Z (GMT). No. of bitstreams: 1 2015 - Vin?cius Menezes Tunholi Alves.pdf: 1609397 bytes, checksum: 1c31fd547893728f1c9c9165ee10137c (MD5) Previous issue date: 2015-07-01
FAPERJ
Angiostrongylus cantonensis Chen, 1935 was first described as a parasite of the pulmonary artery of Rattus norvegicus and Rattus rattus in Canton, China. This helminth is an etiological agent of eosinophilic meningoencephalitis, a zoonotic disease endemic to certain Asian countries, which has recently dispersed to Africa, North and South America, and Caribbean islands. Currently Brazil is considered an area of high risk for the establishment of human angiostrongyliasis, due mainly to the occurrence of naturally infected snails and rodents, acting respectively as definitive and intermediate hosts of this parasite, directly favoring the transmission dynamics of neural angiostrongyliasis. In its life cycle, snails act as intermediate hosts, allowing the development of infective larval forms in the definitive host. In recent years, the metabolic characteristics of experimentally infected snails have been studied in an effort to develop measures to control the pathologies transmitted by these organisms. However, there is little information on the relationship between A. cantonensis and the freshwater snail Biomphalaria glabrata, which is cause for concern, not only because of the importance of this parasite, but also due to the widespread distribution of this snail in Brazil. In this study, the metabolic alterations of B. glabrata resulting from experimental infection by A. cantonensis were evaluated. For this purpose, snails of the pigmented strain were used, reared in the laboratory since oviposition. Two overall groups were formed: control (C1, C2 and C3), with uninfected animals, and treated (I1, I2 and I3), with infected snails. Each subgroup was composed of 10 snails. The entire experiment was performed in duplicate, utilizing a total of 120 snails. After one, two and three weeks of infection, 20 snails from each group were dissected to collect the hemolymph and tissues. The spectrophotometric readings were performed with Doles commercial reagent kits. The biochemical measurement demonstrated that infection by A. cantonensis induced a significant reduction in the hemolymph concentrations of calcium after one week of infection, followed by an increase in the concentrations of this ion in the second week of infection. This situation was accompanied by intense mobilization of CaCO3 in the shell of the infected snails, possibly as an attempt to reestablish the acid-base equilibrium after its alteration during the parasites? development. The histopathology results also demonstrated morphological changes in the digestive gland of infected snails, mainly in the form of granulomatous reactions and areas of metastatic calcification. Variations in the reserves of neutral lipids stored in the digestive gland-gonad complex were also observed. Infection resulted in a significant decrease in the concentrations of cholesterol and an increase in the levels of fatty acid and triacylglycerol after the first two weeks of infection. The increase in the concentration of fatty acid was associated with an increase in lipase activity, indicating that A. cantonensis induces activation of a lipolytic process during development in its intermediate host. Finally, infection by A. cantonensis resulted in changes in the oxidative metabolism of B. glabrata. Besides depletion of the polysaccharide reserves stored in the digestive gland and cephalopodal mass, parasitism by A. cantonensis induced activation of the host?s anaerobic metabolism, resulting not only in an increase in activity of lactate dehydrogenase in the hemolymph, but also reduction of the levels of pyruvic acid and accumulation of lactate. This is an interesting adaptive response of the host to infection, enabling the host, through anaerobic metabolism, to generate energy while still maintaining its redox balance. Additionally, the reduction in the concentrations of oxalic acid in the final parasite development periods suggests its diversion to gluconeogenesis, indicating the involvement of this molecule as a precursor in the synthesis of glucose-6-phosphate. This metabolic condition was accompanied by a deletion in oxidative phosphorylation infected snails (1 and 2 weeks) after infection, suggesting a decrease in the number of mitochondria in the examined tissue, or suppression of enzymatic centers related to oxidative reactions.
O nemat?ide Angiostrongylus cantonensis foi primeiramente descrito como parasito de art?ria pulmonar de Rattus norvegicus e Rattus rattus em Canton, China. Este helminto tem sido mencionado como principal agente etiol?gico da meningoencefalite eosinof?lica humana, uma metazoonose end?mica no continente asi?tico e que recentemente, tem-se disseminado para ?frica, Norte e Sul da Am?rica e Ilhas do Caribe. Atualmente, o Brasil ? considerado ?rea de alto risco ao estabelecimento da angiostrongil?ase humana, justificado n?o somente pela presen?a de moluscos, mas tamb?m roedores naturalmente infectados, que atuam respectivamente como hospedeiros intermedi?rios e definitivos desse parasito, fatores que favorecem diretamente a din?mica de transmiss?o da angiostrongil?ase neural. Em seu ciclo de vida moluscos atuam como hospedeiros intermedi?rios, possibilitando a partir se sua infec??o, o desenvolvimento de formas larvais infectantes ao hospedeiro definitivo. Nos ?ltimos anos, a caracteriza??o de padr?es metab?licos de moluscos infectados experimentalmente, tem sido estudada como base para o desenvolvimento de medidas focadas principalmente no controle de patologias transmitidas por estes organismos. Por?m, quando nos referimos a modelos experimentais utilizando A. cantonensis e Biomphalaria glabrata, os dados ainda s?o escassos, o que preocupa, n?o apenas pela import?ncia do parasito, mas tamb?m pela ampla distribui??o da esp?cie B. glabrata no Brasil. Neste estudo, foram observadas altera??es no metabolismo mineral, lip?dico e oxidativo de B. glabrata, decorrentes da infec??o experimental por A. cantonensis. Para isso, foram utilizados moluscos da linhagem pigmentada criados desde a oviposi??o e mantidos em condi??es laborat?riais. Foram formados dois grupos: controle (C1, C2, C3) com animais n?o infectados e infectados (I1, I2 e I3). Os grupos eram compostos por 10 moluscos. Todo experimento foi feito em duplicata, utilizando um total de 120 moluscos. Ap?s 1, 2 e 3 semanas de infec??o, 20 moluscos de cada grupo eram dissecados para a coleta da hemolinfa e tecidos. As leituras espectrofotom?tricas foram realizadas a partir de kits comercias da marca Doles. As dosagens bioqu?micas demonstraram que a infec??o por A. cantonensis induziu uma diminui??o significativa nos conte?dos hemolinf?ticos de c?lcio ap?s primeira semana de infec??o, seguido por um aumento nas concentra??es desse ?on na segunda semana de estudo. Este cen?rio foi acompanhado por uma intensa mobiliza??o de CaCO3 na concha de moluscos infectados, possivelmente como tentativa em restabelecer o equil?brio ?cido-base alterado durante o desenvolvimento do parasito. Resultados histopatol?gicos demonstraram ainda altera??es morfol?gicas na gl?ndula digestiva de moluscos infectados, caracterizadas principalmente na forma de rea??es granulomatosas e ?reas de calcifica??o metast?tica. Varia??es nas reservas de lip?dios neutros estocados no complexo gl?ndula digestiva-g?noda foram tamb?m demonstradas. A infec??o resultou em um decr?scimo significativo nos conte?dos de colesterol e no aumento dos n?veis de ?cido graxo e triacilglicerol ap?s as duas primeiras semanas de infec??o. O aumento nos conte?dos de ?cido graxo foi associado ao aumento da atividade lip?sica, indicando que A. cantonensis induz de fato a ativa??o de um processo lipol?tico durante etapa de desenvolvimento em seu hospedeiro intermedi?rio. Por fim, a infec??o de A. cantonensis resultou em mudan?as no metabolismo oxidativo de B. glabrata. Al?m da deple??o de reservas polissacar?dicas estocadas na gl?ndula digestiva e massa cefalopodal, o parasitismo por A. cantonensis induziu a ativa??o do metabolismo anaer?bio de seu hospedeiro, resultando n?o apenas no aumento da atividade da lactato desidrogenase hemolinf?tica, mas tamb?m na redu??o dos n?veis de ?cido pir?vico e ac?mulo de lactato. Isso representa uma interessante resposta adaptativa do hospedeiro frente ? infec??o, possibilitando o hospedeiro a partir do metabolismo anaer?bio, gerar energia e ao mesmo tempo mant?m seu balan?o redox. Adicionalmente, a redu??o nas concentra??es de ?cido ox?lico observado nos per?odos finais do desenvolvimento parasit?rio, sugere o seu desvio para a gliconeog?nese, destacando o envolvimento dessa mol?cula como precursora na s?ntese de glicose-6-fosfato. Este cen?rio metab?lico foi acompanhado por uma supress?o na fosforila??o oxidativa de moluscos infectados (1 e 2 semanas) de infec??o, sugerindo uma diminui??o na quantidade de mitoc?ndrias no tecido analisado, ou ainda, na inibi??o de centros enzim?ticos relacionados ?s rea??es oxidativas.
33
Oliveira, Camila Krug de. "Express?o diferencial de prote?nas durante a matura??o sexual de Angiostrongylus cantonensis em infec??o experimental de Rattus norvegicus." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2012. http://tede2.pucrs.br/tede2/handle/tede/5451.
Full textAbstract:
Made available in DSpace on 2015-04-14T14:51:22Z (GMT). No. of bitstreams: 1
444695.pdf: 624430 bytes, checksum: e4547549fd33f8db2dfcdef98c9ca071 (MD5)
Previous issue date: 2012-08-31The two parasites in the genus Angionstrongylus that cause disease in humans are Angiostrongylus costarisensis and Angiostrongylus cantonensis. They have different target organs: A. costarisensis is located in the mesentery and causes eosinophilic ileocolitis, whereas A. cantonensis is a neurotropic parasite responsible for eosinophilic meningoencephalitis. Based on several indications that the sexual maturation is associated with increased pathogenesis in angiostrongylid worms, proteomic analysis was performed on A. cantonensis protein samples, to describe differential protein expression between 21 and 42 days post infection. Triplicated bidimensional electrophoresis was submitted to analysis and 11 proteins were found to be exclusively expressed after sexual maturation. Acetate kinase was the only protein that could be identified after mass spectrometry (LC-MS/MS). Since acetate is an important end-product of the energy metabolism among many parasites but not among their mammalian hosts, acetate formation is an attractive target for the development of new anti-parasitic drugs. Furthermore, studying the adaptations in parasite metabolisms can result in an increased understanding of the host-parasite interaction. This data open opportunities for control interventions and new strategies for molecular diagnosis.
Os principais parasitos do g?nero Angionstrongylus que causam doen?a nos seres humanos s?o Angiostrongylus costarisensis e Angiostrongylus cantonensis. Eles t?m distintos ?rg?os alvos: A. costarisensis est? localizado no mesent?rio e causa ileocolite eosinof?lica, enquanto que A. cantonensis ? um parasita neurotr?pico respons?vel pela meningoencefalite eosinof?lica. Com base em v?rias evid?ncias de que a matura??o sexual est? associada ao aumento da patog?nese em vermes deste g?nero, uma an?lise prote?mica foi realizada em vermes de A. cantonensis para descrever a express?o diferencial de prote?nas entre 21 e 42 dias ap?s a infec??o. Eletroforese bidimensional em triplicata foi submetida ? an?lise e 11 prote?nas foram encontradas exclusivamente expressas ap?s a matura??o sexual. A acetato quinase foi a ?nica prote?na identificada por espectrometria de massa (LC-MS/MS). O acetato ? um importante produto final do metabolismo energ?tico de muitos parasitos, mas n?o de seus hospedeiros mam?feros. Sendo assim, ? um alvo atrativo para o desenvolvimento de novas drogas anti-parasit?rias. Al?m do mais, o estudo das adapta??es no metabolismo do parasito pode resultar em uma maior compreens?o da rela??o parasito-hospedeiro. Este trabalho abre oportunidades para interven??es de controle e novas estrat?gias de diagn?stico molecular.
34
Morassutti, Alessandra Loureiro. "Estudo de proteínas de Angiostrongylus cantonensis para o entendimento da relação parasito-hospedeiro e análise de alvos para o diagnóstico das angiostrongilíases." Pontifícia Universidade Católica do Rio Grande do Sul, 2011. http://hdl.handle.net/10923/5350.
Full textAbstract:
Made available in DSpace on 2013-08-07T19:12:27Z (GMT). No. of bitstreams: 1
000437829-Texto+Completo-0.pdf: 3097644 bytes, checksum: 63052063f3fb08648c7ca5a4506262bc (MD5)
Previous issue date: 2011The genus Angiostrongylus Kamensky, 1905 belongs to the Phylum Nematode, with round shape as its main feature. Two species have medical importance, A. costaricensis living in mesenteric arteries of wild mice and causing abdominal angiostrongyliasis in human and A. cantonensis which lives in pulmonary arteries of rats and may cause eosinophilic meningoencephalitis in humans. The diagnosis of both diseases is difficult due to absence of parasite in feces in case of the infection by A. costaricensis and seldom detected larvae in the cerebrospinal fluid in case of eosinophilic meningoencephalitis. Several studies have been performed to improve the diagnosis of angiostrongyliasis which should be able to differentiate in a specific and sensitive way among other parasitic infections. The 31kDa antigen has been considered the main antigen for eosinophilic meningoencephalitis diagnosis due to A. cantonensis infection. However this antigen is obtained from crude extracts of the worm by a laborious process of purification with low yielding and insufficient amount for large distribution to other diagnostic centers. In order to improve the serologic diagnostic of angiostrongyliasis and make the antigens widely available the present work aimed to identify new antigenic targets and also characterize the 31kDa antigen for further recombinant production. Besides that, essential molecules for parasite survival were investigated which in the future may be targets for disease treatment. Two sources of antigen from female worms were used: excretion and secretion products (ES) and total extract (TE). In ES, sample antioxidant enzymes activity were detected such as catalase and superoxide dismutase. Also was identified by Western blot and Mass spectrometry (MS), 17 proteins target for disease diagnosis and treatment like hemoglobinases, heat shock proteins and proteases inhibitors. In TE sample antioxidant enzymes as well as glutathione transferases (GST) which is another kind of defense enzyme were also detected. GSTs were purified by affinity chromatography and analyzed by MS. Peptide sequences from this experiment matched with homologous sequences of sigma class GST. In TE samples was possible to characterize the 31kDa and after two-dimensional electrophoresis was shown to be composed of four spots around 4. 5 of isoelectric point (pI) and being recognized by sera from patients infected with Angiostrongylus spp. The spots were analyzed by MS and three different proteins were identified: 14-3-3 protein, NAC domain containing protein, and epsilon subunit of the coatomer protein complex isoform 2. The 31kDa antigen was characterized as a glycoprotein through studies of oxidation of carbohydrate where it was observed that the antigenicity of four spots was dependent on sugar residues. The DNA sequences of the antigens were obtained by random sequencing of the genome for 454 platform (Roche) and deposited in Genbank. The data generated in this study contribute significantly to the development of recombinant antigens that may be widely distributed for independent diagnostic validation.
O gênero Angiostrongylus Kamensky, 1905 agrupa animais pertencentes ao filo Nematoda, cuja característica marcante é a forma corporal cilíndrica. Duas espécies possuem importância médica: A. costaricensis cujo habitat natural são as artérias mesentéricas de camundongos silvestres e na infecção humana pode levar ao desenvolvimento de angiostrongilíase abdominal; e A. cantonensis que habita as artérias pulmonares de roedores e na infecção humana pode causar meningoencefalite eosinofílica. O diagnóstico de ambas as doenças é dificultado pela ausência de formas parasitárias nas fezes, no caso de infecções por A. costaricensis e raramente encontradas no líquido cefaloraquidiano no caso de meningoencefalite eosinofílica. Muitos estudos vêm sendo desenvolvidos para o aprimoramento da detecção das angiostrongilíases visando testes que sejam capazes de discernir das diferentes infecções parasitárias de forma sensível e específica. O antígeno de 31kDa é considerado atualmente o principal antígeno para o diagnóstico da meningoencefalite eosinofílica, causada por A. cantonensis, entretanto é proveniente da purificação de extratos brutos do parasito o que acarreta num processo laborioso e dispendioso que em última análise gera quantidades insuficientes para que haja ampla distribuição e compartilhamento entre os centros de diagnóstico. Com o intuito de aprimorar o diagnóstico sorológico das angiostrongilíases e tornar os antígenos disponíveis globalmente o presente trabalho buscou identificar novos alvos antigênicos e caracterizar o antígeno de 31kDa para posterior propagação de formas recombinantes. Além disso, foram estudadas moléculas que podem ser fundamentais na manutenção do parasitismo, que futuramente poderão ser alvos para o tratamento das angiostrongilíases. Duas fontes de antígenos a partir de vermes adultos fêmeas foram empregadas: produtos de excreção e secreção (ES) e extrato bruto (TE). Nos ES foi detectada a atividade de enzimas antioxidantes como catalase e superóxido dismutase e identificadas, por western blot e espectrometria de massas (MS), 17 proteínas alvo para o diagnóstico e tratamento das angiostrongilíases dentre elas hemoglobinases, proteínas de choque térmico e inibidores de proteases. Nas amostras de TE além da identificação de enzimas antioxidantes, estavam presentes glutationas transferases (GST), outra classe de enzimas de defesa. Estas proteínas foram purificadas por cromatografia de afinidade e analisadas por MS o que revelou sequencias peptídicas homólogas a GST de classe sigma. Em TE também foi possível a caracterização do antígeno de 31kDa que quando submetido a eletroforese bidimensional mostrou-se ser composto por 4 spots com ponto isoelétrico (pI) em torno de 4,5 sendo reconhecidos pelo soro de pacientes infectados com Angiostrongylus spp. Os spots foram analisados por MS e três diferentes proteínas foram identificadas: 14-3-3; proteína com domínio NAC e a subunidade épsilon do coatamero. O antígeno de 31kDa foi caracterizado como uma glicoproteína através de estudos de oxidação de glicídeos, onde se observou que a antigenicidade dos 4 spots foi dependente de resíduos de açúcar. As sequências de DNA dos antígenos foram obtidas pelo sequenciamento aleatório do genoma pela plataforma 454 (Roche) e depositadas no Genbank. Os dados gerados no presente trabalho contribuem de forma significativa para o desenvolvimento de antígenos recombinantes que poderão ser amplamente distribuídos para validação e aplicação em diagnóstico.
35
BOMFIM, Tatiane Cristina dos Santos. "Avalia??o do perfil glic?dico e da biologia reprodutiva de Biomphalaria glabrata (Mollusca) experimentalmente co-infectada por Angiostrongylus cantonensis (Nematoda) e Echinostoma paraensei (Trematoda)." Universidade Federal Rural do Rio de Janeiro, 2016. https://tede.ufrrj.br/jspui/handle/jspui/1571.
Full textAbstract:
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-05-02T21:37:15Z
No. of bitstreams: 1
2016 - Tatiane Cristina dos Santos Bonfim.pdf: 2465996 bytes, checksum: 94f74695ec8fd235bfbcd7f4a82aec87 (MD5)Made available in DSpace on 2017-05-02T21:37:15Z (GMT). No. of bitstreams: 1 2016 - Tatiane Cristina dos Santos Bonfim.pdf: 2465996 bytes, checksum: 94f74695ec8fd235bfbcd7f4a82aec87 (MD5) Previous issue date: 2016-02-25
CNPq
The interaction between intermediate hosts and helminths can cause metabolic and reproductive changes in the host snail, they start using their reserves to maintain its vital functions, immune system activation, repair of tissue damage and also to supply the necessary energy for the parasites development. Investigations of co-infection by different helminths can provide essential information about the biology of their co-existence. Our aims are investigate the reproductive changes, evaluate the activity of lactate dehydrogenase and the concentrations of glucose in the hemolymph and glycogen in the digestive gland complex and cephalopedal mass, and also verify histological and histochemical changes of Biomphalaria glabrata experimentally co-infected with Echinostoma paraensei and Angiostrongylus cantonensis. Five groups of snails i.e. uninfected, with single and double infections, either E. paraensei first (E+A) or A. cantonensis first (A+E) were followed; three times a week during four weeks the numbers of egg masses, eggs and hatched snails were counted. Histological and histochemical samples of the ovotestis, albumen gland, digestive gland complex and cephalopedal mass were collected after four weeks as well samples for the biochemical analysis. The number of egg masses/snail, eggs/snail and hatched snails showed significant differences comparing the control group to all infected groups, especially in group E + A, with the majority of values of parameters analyzed lower than 50% of those observed for control snails. A significant decrease in glucose levels and glycogen content, in contrast an increase of lactate dehydrogenase activity was observed in the infected snails. These results indicate intense energy demand that takes the snail increasing the anaerobic degradation of carbohydrates in the infected snails to obtain energy in an attempt to maintain homeostasis. The histological analysis showed that presence of both parasites in the all organs analyzed and the parasites were associated with cell disorganization.
A intera??o entre hospedeiros intermedi?rios e helmintos pode causar altera??es metab?licas e reprodutivas no molusco hospedeiro, que come?am a usar suas reservas para manter as fun??es vitais, na elabora??o de resposta imunol?gica e repara??o de danos teciduais e tamb?m para compensar a drenagem de nutrientes/energia pelos parasitos em desenvolvimento. Investiga??es sobre as co-infec??es por helmintos de esp?cies diferentes podem fornecer informa??es essenciais sobre a biologia da sua co-exist?ncia, refletindo aspectos mais pr?ximos ?queles que encontramos em condi??es naturais. Nossos objetivos foram investigar as altera??es reprodutivas, avaliar a atividade da lactato desidrogenase e as concentra??es de glicose na hemolinfa e glicog?nio no complexo gl?ndula digestiva-g?nada e massa cefalopediosa, e tamb?m verificar altera??es histol?gicas e histoqu?micas de Biomphalaria glabrata experimentalmente co-infectados com Echinostoma paraensei e Angiostrongylus cantonensis. Cinco grupos de moluscos (n?o infectados, com infec??es simples (Echinostoma paraensei ou Angiostrongylus cantonensis) e dupla, seja E. paraensei primeiro (E + A) ou A. cantonensis primeiro (A + E)) foram acompanhados; tr?s vezes por semana durante quatro semanas o n?mero de massas de ovos, n?mero de ovos e n?mero de moluscos eclodidos foram contados. Amostras para a an?lise histol?gica e histoqu?mica do ovoteste, gl?ndula do alb?men, complexo gl?ndula digestiva e massa cefalopediosa foram coletadas ap?s quatro semanas, assim como amostras para a an?lise bioqu?mica. O n?mero de massas de ovos/molusco, ovos/molusco e moluscos eclodidos dos moluscos infectados apresentaram diferen?as significativas quando comparados com o grupo controle, especialmente no grupo E + A, cuja a maioria dos valores dos par?metros analisados foi inferior a 50% dos valores observados para os moluscos controle. Um decr?scimo significativo nos n?veis de glicose e de glicog?nio, em contraste com um aumento de atividade da lactato desidrogenase foi observado nos exemplares infectados. Estes resultados indicam que a demanda de energia intensa leva o molusco infectado ao aumento da degrada??o anaer?bia de carboidratos para obten??o de energia buscando atender ao aumento da demanda energ?tica, numa tentativa de manter a homeostase glic?mica, por?m redu??es significativas s?o observadas. As an?lises histol?gicas e histoqu?micas mostraram a presen?a de ambos os parasitos nos ?rg?os analisados associados com intensa desorganiza??o celular.
36
Fontanilla, Ian Kendrich C. "Achatina (Lissachatina) fulica Bowdich : its molecular phylogeny, genetic variation in global populations, and its possible role in the spread of the rat lungworm Angiostrongylus cantonensis (CHEN)." Thesis, University of Nottingham, 2010. http://eprints.nottingham.ac.uk/11069/.
Full textAbstract:
The Giant African Snail, Achatina (Lissachatina) fulica Bowdich, is a tropical crop pest species with a widespread distribution across East Africa, the Indian subcontinent, Southeast Asia, the Pacific and the Caribbean. It is also a known intermediate host of the rat lungworm, Angiostrongylus cantonensis, which can infect humans and cause eosinophilic meningitis. The phylogenetic position of A. fulica within the Achatinoidea and the Achatinidae was investigated using segments of the nuclear ribosomal (r) RNA cluster, actin and histone 3 genes and the mitochondrial CO1 and 16S rRNA genes. Results from molecular data support the monophyly of the Achatinidae based on the taxa surveyed as well as the morphological distinction of the Eastern Achatina (Lissachatina) from the Western and Central Achatina (Achatina); Lissachatina should therefore be elevated to genus status. The results also show non-monophyly of the Coeliaxidae, Ferussaciidae and Subulinidae; the taxonomy of these families must therefore be reassessed. The extent of genetic diversity in global A. fulica populations was also determined using an SSCP molecular marker developed from the 16S rRNA gene. Results reveal only one haplotype (C) emerged from East Africa and spread globally. The rat lungworm (Angiostrongylus cantonensis) has a parallel distribution with A. fulica, and the possible role of the snail in the spread of the parasite is investigated using a molecular marker derived from the small subunit (SSU) rRNA gene. A survey of the parasite within the route of dispersal of A. fulica detected A. cantonensis only in the Philippines and the French Polynesian territory of Tahiti, the latter of which being the first reported case of A. cantonensis infection for Achatina fulica in that territory. Due to the limited sampling of the snail and the patchy distribution of the parasite, there are insufficient data at this time to assess the role of Achatina fulica in the spread of Angiostrongylus cantonensis.
37
Morassutti, Alessandra Loureiro. "Estudo de prote?nas de Angiostrongylus cantonensis para o entendimento da rela??o parasito-hospedeiro e an?lise de alvos para o diagn?stico das angiostrongil?ases." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2011. http://tede2.pucrs.br/tede2/handle/tede/225.
Full textAbstract:
Made available in DSpace on 2015-04-14T13:09:31Z (GMT). No. of bitstreams: 1
437829.pdf: 3097644 bytes, checksum: 63052063f3fb08648c7ca5a4506262bc (MD5)
Previous issue date: 2011-12-16The genus Angiostrongylus Kamensky, 1905 belongs to the Phylum Nematode, with round shape as its main feature. Two species have medical importance, A. costaricensis living in mesenteric arteries of wild mice and causing abdominal angiostrongyliasis in human and A. cantonensis which lives in pulmonary arteries of rats and may cause eosinophilic meningoencephalitis in humans. The diagnosis of both diseases is difficult due to absence of parasite in feces in case of the infection by A. costaricensis and seldom detected larvae in the cerebrospinal fluid in case of eosinophilic meningoencephalitis. Several studies have been performed to improve the diagnosis of angiostrongyliasis which should be able to differentiate in a specific and sensitive way among other parasitic infections. The 31kDa antigen has been considered the main antigen for eosinophilic meningoencephalitis diagnosis due to A. cantonensis infection. However this antigen is obtained from crude extracts of the worm by a laborious process of purification with low yielding and insufficient amount for large distribution to other diagnostic centers. In order to improve the serologic diagnostic of angiostrongyliasis and make the antigens widely available the present work aimed to identify new antigenic targets and also characterize the 31kDa antigen for further recombinant production. Besides that, essential molecules for parasite survival were investigated which in the future may be targets for disease treatment. Two sources of antigen from female worms were used: excretion and secretion products (ES) and total extract (TE). In ES, sample antioxidant enzymes activity were detected such as catalase and superoxide dismutase. Also was identified by Western blot and Mass spectrometry (MS), 17 proteins target for disease diagnosis and treatment like hemoglobinases, heat shock proteins and proteases inhibitors. In TE sample antioxidant enzymes as well as glutathione transferases (GST) which is another kind of defense enzyme were also detected. GSTs were purified by affinity chromatography and analyzed by MS. Peptide sequences from this experiment matched with homologous sequences of sigma class GST. In TE samples was possible to characterize the 31kDa and after two-dimensional electrophoresis was shown to be composed of four spots around 4.5 of isoelectric point (pI) and being recognized by sera from patients infected with Angiostrongylus spp. The spots were analyzed by MS and three different proteins were identified: 14-3-3 protein, NAC domain containing protein, and epsilon subunit of the coatomer protein complex isoform 2. The 31kDa antigen was characterized as a glycoprotein through studies of oxidation of carbohydrate where it was observed that the antigenicity of four spots was dependent on sugar residues. The DNA sequences of the antigens were obtained by random sequencing of the genome for 454 platform (Roche) and deposited in Genbank. The data generated in this study contribute significantly to the development of recombinant antigens that may be widely distributed for independent diagnostic validation
O g?nero Angiostrongylus Kamensky, 1905 agrupa animais pertencentes ao filo Nematoda, cuja caracter?stica marcante ? a forma corporal cil?ndrica. Duas esp?cies possuem import?ncia m?dica: A. costaricensis cujo habitat natural s?o as art?rias mesent?ricas de camundongos silvestres e na infec??o humana pode levar ao desenvolvimento de angiostrongil?ase abdominal; e A. cantonensis que habita as art?rias pulmonares de roedores e na infec??o humana pode causar meningoencefalite eosinof?lica. O diagn?stico de ambas as doen?as ? dificultado pela aus?ncia de formas parasit?rias nas fezes, no caso de infec??es por A. costaricensis e raramente encontradas no l?quido cefaloraquidiano no caso de meningoencefalite eosinof?lica. Muitos estudos v?m sendo desenvolvidos para o aprimoramento da detec??o das angiostrongil?ases visando testes que sejam capazes de discernir das diferentes infec??es parasit?rias de forma sens?vel e espec?fica. O ant?geno de 31kDa ? considerado atualmente o principal ant?geno para o diagn?stico da meningoencefalite eosinof?lica, causada por A. cantonensis, entretanto ? proveniente da purifica??o de extratos brutos do parasito o que acarreta num processo laborioso e dispendioso que em ?ltima an?lise gera quantidades insuficientes para que haja ampla distribui??o e compartilhamento entre os centros de diagn?stico. Com o intuito de aprimorar o diagn?stico sorol?gico das angiostrongil?ases e tornar os ant?genos dispon?veis globalmente o presente trabalho buscou identificar novos alvos antig?nicos e caracterizar o ant?geno de 31kDa para posterior propaga??o de formas recombinantes. Al?m disso, foram estudadas mol?culas que podem ser fundamentais na manuten??o do parasitismo, que futuramente poder?o ser alvos para o tratamento das angiostrongil?ases. Duas fontes de ant?genos a partir de vermes adultos f?meas foram empregadas: produtos de excre??o e secre??o (ES) e extrato bruto (TE). Nos ES foi detectada a atividade de enzimas antioxidantes como catalase e super?xido dismutase e identificadas, por western blot e espectrometria de massas (MS), 17 prote?nas alvo para o diagn?stico e tratamento das angiostrongil?ases dentre elas hemoglobinases, prote?nas de choque t?rmico e inibidores de proteases. Nas amostras de TE al?m da identifica??o de enzimas antioxidantes, estavam presentes glutationas transferases (GST), outra classe de enzimas de defesa. Estas prote?nas foram purificadas por cromatografia de afinidade e analisadas por MS o que revelou sequencias pept?dicas hom?logas a GST de classe sigma. Em TE tamb?m foi poss?vel a caracteriza??o do ant?geno de 31kDa que quando submetido a eletroforese bidimensional mostrou-se ser composto por 4 spots com ponto isoel?trico (pI) em torno de 4,5 sendo reconhecidos pelo soro de pacientes infectados com Angiostrongylus spp. Os spots foram analisados por MS e tr?s diferentes prote?nas foram identificadas: 14-3-3; prote?na com dom?nio NAC e a subunidade ?psilon do coatamero. O ant?geno de 31kDa foi caracterizado como uma glicoprote?na atrav?s de estudos de oxida??o de glic?deos, onde se observou que a antigenicidade dos 4 spots foi dependente de res?duos de a??car. As sequ?ncias de DNA dos ant?genos foram obtidas pelo sequenciamento aleat?rio do genoma pela plataforma 454 (Roche) e depositadas no Genbank. Os dados gerados no presente trabalho contribuem de forma significativa para o desenvolvimento de ant?genos recombinantes que poder?o ser amplamente distribu?dos para valida??o e aplica??o em diagn?stico
38
Moreira, Vera Lúcia Coimbra. "Helmintofauna com potencial zoonótico em ratos urbanos: uma análise em bairros de Belém-PA." Universidade Federal do Amazonas - Universidade Federal do Pará, 2010. http://tede.ufam.edu.br/handle/tede/4553.
Full textAbstract:
Submitted by Geyciane Santos (geyciane_thamires@hotmail.com) on 2015-08-07T14:36:36Z
No. of bitstreams: 1
Dissertação - Vera Lúcia Coimbra Moreira.pdf: 173676939 bytes, checksum: 643c5b8525ed51ecdce8f978186dd9ce (MD5)Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-08-11T15:41:19Z (GMT) No. of bitstreams: 1 Dissertação - Vera Lúcia Coimbra Moreira.pdf: 173676939 bytes, checksum: 643c5b8525ed51ecdce8f978186dd9ce (MD5)
Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-08-11T15:48:04Z (GMT) No. of bitstreams: 1 Dissertação - Vera Lúcia Coimbra Moreira.pdf: 173676939 bytes, checksum: 643c5b8525ed51ecdce8f978186dd9ce (MD5)
Made available in DSpace on 2015-08-11T15:48:05Z (GMT). No. of bitstreams: 1 Dissertação - Vera Lúcia Coimbra Moreira.pdf: 173676939 bytes, checksum: 643c5b8525ed51ecdce8f978186dd9ce (MD5) Previous issue date: 2010-12-06
CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Urban rodents are denominated sinanthropes, commensal, due to their assosciation to human species and for depend of anthropic environment for survive. Historically, these animals are recognized as transmitter’s agents of diseases for humans and domestic animals, since Black Death, in XIV century. However, urban rats transmit many other parasites beyond the bacteria Yersinia pestis, they hot and transmit helminths, however more studies must be developed, after Abu-Madi (2005). The present work intends to accomplish a survey of the helminth fauna of urban rats with zoonotic potential in the districts of Belém-Pa. In the year of preparation of the project (2008), Guamá and Montese (Terra Firme) districts were selected, these two districts with a high index of leptospirosis (SINAN, 2007) and with poor sanitation and garbage accumulation close to houses (clues to rodents presence). In these districts, 16 rodents of genus Rattus were captured, of distinct sex and reproductive age. Captured animals were necropsied at Biotério of ICB/UFPA and helminths found were colleted for studies and identification of species at LBCH-UFPA. Two of the species identified with zoonotic potential, Calodium hepaticum and Angiostrongylus cantonensis, have a worldwide distribution and have Rattus rattus and R. norvegicus as main definitive hosts. Calodium hepaticum is a trichurid nematode that parasitizes the hepatic parenchyma, with capacity to infect many species of mammals, including human. However, this finding is rare. Angiostrongylus cantonensis is a parasite of the pulmonar artery of rodents that, in humans, have their life cycle interrupted during the passage to central nervous system, giving risc to a local inflammatory process known as eosinophilic meningitis, a disease considered endemic in asian countries, with reported cases in Brazil, in Espirito Santo and Pernambuco. This is the first record of these helminths in rats from Belém, that contributes for mapping of occurrence these nematodes and serves as alerts to public health about these zoonosis that urban population and their visitors are exposed.
Roedores urbanos são também denominados sinantrópicos comensais devido a sua associação à espécie humana e, dependência do ambiente antrópico para sua sobrevivência. Historicamente, estes animais são reconhecidos como agentes transmissores de doenças aos homens e aos animais domésticos, desde a disseminação da Peste Negra, no século XIV. Entretanto, ratos urbanos veiculam muitos outros parasitos além da bactéria Yersinia pestis, albergam e transmitem helmintos, aos quais devem ser dedicados maiores estudos, segundo Abu-Madi (2005). Este trabalho objetivou fazer um levantamento da helmintofauna com potencial zoonótico de ratos urbanos em bairros de Belém-PA. À época da elaboração do projeto (2008), foram selecionados Guamá e Montese (Terra Firme), os dois bairros com os índices mais elevados de leptospirose (SINAN, 2007) e com saneamento urbano precário e acúmulo de lixo próximo às casas (indícios da presença dos roedores). Nestes bairros, foram capturados 16 roedores do gênero Rattus, de sexo e idade reprodutiva distintas. Os animais capturados foram necropsiados no Biotério do ICB/UFPA e os helmintos encontrados, retirados para estudo e identificação de espécie no LBCH-UFPA. Duas das espécies identificadas, Calodium hepaticum e Angiostrongylus cantonensis, possuem reconhecido potencial zoonótico de ocorrência mundial, apresentando Rattus rattus e R. norvegicus como principais hospedeiros definitivos. Calodium hepaticum é um nematódeo trichurideo, parasito de parênquima hepático, com capacidade de infectar diversas espécies de mamíferos, incluindo a espécie humana, embora este seja um achado considerado raro. Angiostrongylus cantonensis é um parasito de artéria pulmonar de roedor que, no homem, tem seu ciclo interrompido na passagem pelo sistema nervoso central, desencadeando um processo inflamatório local, conhecido como meningite eosinofílica, enfermidade considerada endêmica em países asiáticos, com casos relatados no Brasil, nos estados do Espírito Santo e Pernambuco. Este é o primeiro registro da ocorrência de ambos os helmintos circulando no município de Belém, através de ratos, o que contribui para o mapeamento da ocorrência destes nematódeos e serve de alerta às instituições de controle de endemias, do risco para a saúde pública sobre estas zoonoses as quais a população da cidade e seus visitantes estão expostos.
39
Chen, Kuang Yao, and 陳光耀. "Comparative proteomics of Angiostrongylus cantonensis." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/09459397411014047200.
Full textAbstract:
碩士長庚大學
生物醫學研究所
98
Angiostrongylus cantonensis is a parasite of rats. It is an important causative agent of eosinophilic meningitis and eosinophilic menigoencephalisis in humans. This study was conducted to investigate the alternations of proteomes on the third- and fifth-stage larvae of A.cantonensis by proteomic techniques. Proteins with molecular weight of 15-35 kDa and pI 5-6 were mainly revealed in the third-stage larvae whereas those of 40-100 kDa and pI 5-7 in the female fifth-stage larvae. Of 119 protein spots identified by MALDI-TOF MS and database searches, 100 were found to have significant similarities with existing proteins in the NCBI database: 33 from the third-stage larvae and 67 from the fifth-stage larvae. After matching with the GO (gene ontology) database, the functions of 35 spots (6 from the third-stage larvae and 29 from the fifth-stage larvae) were determined. According to their functions, these spots were classified into 12 groups and stress-related proteins were found to be the most abundant. The high expression level of stress-related proteins reflects that the larvae of A. cantonensis need to adapt the new environment and the resist the host immune responses after entering the new host.
40
Chang, Shih-Hsin, and 張世欣. "Cloning and characterization of Secreted Protease in Angiostrongylus cantonensis." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/60993101411259170115.
Full textAbstract:
碩士長庚大學
基礎醫學研究所
93
Aspartic proteases play a pivotal role when the helminthes invade the host. Helminth aspartic proteases facilitate the invasion of host tissue and digest host hemolglobin as a food source. An aspartic protease homologues EST, AcL5-C160 has been isolated form our Angiostrongylus cantonensis EST library. 5’RACE was used to obtain the full length mRNA of this gene. The complete open reading frame（ORF）of the AcL5-C160 is 1,278 bp. The putative ORF encode protein of 425 amino acid residues with predicted molecular weight of 46 kDa, isoelectric point 5.8. The amino acid sequence of this aspartic protease from A. cantonensis showed similarity between 42–52% with Caenorhabditis elegans、Necator americanus and Haemonchus contortus. 3D model also showed similarity by ExPASy Molecular Biology Sever. We forecast the antigenic sequence and synthesize a 15 amino acids polypeptide. Rabbits were immunized with this synthetic polypeptide and obtained polyclonal antibodies. Western blotting was used to recognize the AcL5-C160 aspartic protease. We find aspartic proteases may exist in the somatic antigen and excretory/secretory products (ESP) of the fifth-stage larvae of A.cantonensis.
41
Fu, Cha-Hui, and 富家蕙. "Immunological analyses of intestinal proteins extracted from adult Angiostrongylus cantonensis." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/38632297445569090429.
Full textAbstract:
碩士國立中山大學
生物科學系研究所
89
Abstract In order to determine whether antigens prepared from adult intestine of Angiostrongylus cantonensis have potential to induce a protective immunity in the rodent hosts, somatic antigens extracted from male and female adults as well as gut antigens isolated from female adults were used to immunize rats against A. cantonensis infection. A 14% reduction in L5 recorvery from brain as well as a 15% reduction in adult recovery from pulmonary artery were achieved in the immunized rats when compared with the control group after infection with 50 larvae. The length of worms recovered from immunized rats was shorter than that in other groups. The larvae recovered from fecal materials in immunized rats were also reduced. In cell proliferation test, the stimulation index of gut antigens increased with times of immunization and exhibited the highest values. However, serum IgG titers were not correlated with protective immune responses. A 84 kDa protein contained in all antigen preparations was recognized by immune serum against gut antigens. Strong positive reactions were detected by indirect immunofluorescent assay in the internal musculature of the body, gut and reproductive tract wall and gut lumen. The composition of gut antigens was similar to that of gut membrane proteins. Immune sera recognized several major gut proteins were also appeared on gut membrane proteins. Further studies are required to provide evidence that gut membrane proteins play in the protective immune response against A. cantonensis infections, including the 84 kDa protein.
42
Lan, Kuang-Ping, and 蘭光坪. "The mechanisms of eosinophilia in mice infected with Angiostrongylus cantonensis." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/64820603047355800916.
Full textAbstract:
博士中山醫學大學
生化暨生物科技研究所
93
Eosinophilic meningitis or meningoencephalitis caused by Angiostrongylus cantonensis is endemic to the Pacific area of Asia, especially Taiwan, Thailand, and Japan. Although eosinophilia is an important clinical manifestation of A. cantonensis infection, the role of eosinophils in the progress of the infection remains to be elucidated. In this experiment, we show that A. cantonensis-induced eosinophilia and inflammation might lead to the induction of IAP/NF-κB, JAK/STAT1 and MEKK1/JNK signals. The phosphorylation levels of JAK and JNK, STAT1, IAP, NF-κB and MEKK1 protein products were significantly increased by 4.7-, 13-, 1.9-, 11.3-, 10.3- and 2.0- fold, respectively, after 12 days or 15 days of A. cantonensis infection. However, no significant differences in MAPKs such as Raf, MEK-1, ERK1/2 and p38 expression were found between control and infected mice. The activation potency of JAK/STAT1, IAP/NF-κB and MEKK1/JNK started increasing on day 3, significant induction on days 12 or day 15 after A. cantonensis infection. Consistent results were noted in the pathological observations, including eosinophilia, leukocyte infiltration, granulomatous reactions, and time responses in the brain tissues of infected mice. These data suggest that the development of brain injury by eosinophilia of A. cantonensis infection is associated with activation of JAK/STAT1 signals by cytokines, and/or activation of MEKK1/JNK by oxidant stress, and/or activation of NF-κB by increasing IAP expression.
43
Chen, Cheng-Zhi, and 陳程志. "Mitochondria dynamic in the mouse brain infected by Angiostrongylus cantonensis." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/47698694356822259841.
Full textAbstract:
碩士慈濟大學
醫學生物技術碩士班
105
Angiostrongylus cantonensis, the rat lungworm, is the major cause of eosinophilic meningitis. Rats are the definitive host of this nematode. Human can be infected by eating the third larvae from intermediate hosts. The symptoms of eosinophilic meningitis are meningitis and eosinophilic pleocytosis. Mitochondrial dynamic is crucial for the regulation of cell homeostasis. In this study, we attempted to assess the direct effects of A. cantonensis infection on mice brain, and to explore the expression of mitochondrial morphology, dynamics, and function. There were 5 groups (normal and 1, 2, 3 and 4 week post infection) of mice (5 mice in each group and each mice was infected with 25 L3 of A. cantonensis). Fission of mitochondria can be observed by TEM (transmission electronic microscopy). The brain cells were severely damaged and the mitochondria of showed atrophy at third week post infection. By western blotting, the protein of mitochondrial fission, biogenesis and mitophagy was increased, and the fussion protein was decreased at second and third week post infection. The protein of apoptosis (caspase 8 and 9) had significant increasd. Tthe mouse meningeal after infection can be found inflammation and damage in the sections of the brain tissue. Larvae were embedded in the cortex, and cell infiltration could be found around larvae. In TUNEL assay, the number of apoptotic cells was increased in the mice brain at second week post infection, and brain cell necrosis was more serious at third and fourth week post infection. It was also found that brain cell necrosis was increased in infected mice by Annexin V test. For mitochondrial function, membrane potential was detected. It was increased after infection, but it could not be detected at fourth week post infection. This may due to cell necrosis and therefore membrane potential could not be detected. These results confirmed that the A. cantonensis infection can influence mitochondrial dynamic, biogenesis, morphology and function in mice model.
44
Tsai, Hung-Chin, and 蔡宏津. "Clinical and pathogenetic study of eosinophilic meningitis caused by Angiostrongylus cantonensis." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/71337093629014779725.
Full textAbstract:
博士高雄醫學大學
醫學研究所博士班
96
Angiostrongylus cantonensis is the most common cause of eosinophilic meningitis worldwide. Human infection occurs following ingestion of the worms in raw snails or fish that serve as intermediate hosts. Three outbreaks of central nervous system infection with A. cantonensis occurred in Kaoshiung, Taiwan during 1998, 1999 and 2001 among 17 Thai laborers who ate raw snails and 5 native Taiwanese who drank raw vegetable juice. We performed a retrospective cohort study to enhance case findings with eosinophilic meningitis, as well as environmental surveillance of larvae in snails and testing for antibodies of A. cantonensis in the blood and cerebrospinal fluids. We enrolled 22 patients with severe headache and eosinophilia within 4 to 90 days after eating raw snails and drinking raw vegetable juice. Sixteen (73 %) developed eosinophilic meningitis. Third stage larvae were found in the cerebrospinal fluids of 2 patients and 12/12 snails. Specific antibodies were detected in serum and cerebrospinal fluids. The severity of illness and eosinophilia were associated with amount of snails ingested. Eighteen of twenty-two of these cases underwent magnetic resonance imaging (MRI) of the brain. MRI imaging revealed high signal intensities over the globus pallidus on TI-weighted imaging, leptomeningeal enhancement, ventriculomegaly and punctate areas of abnormal enhancement within the cerebral and cerebellar hemisphere on gadolinium enhancing T1 imaging, and a hyperintense signal on T2-weighted images. There was a significant correlation between severity of headache, CSF pleocystosis and CSF and blood eosinophilia with MRI signal intensity in T1-weighted imaging (p <0.05). Among the five native Taiwanese who drank raw vegetable juice, risk factors identification showed that consumption of raw vegetable juice was associated with illness (Pearson correlation test r = 0.867, P= 0.01). There was association between the presence of raw vegetable juice and CSF eosinophilia (Spearman’s correlation test r=0.816, P= 0.004). Vascular endothelial growth factor (VEGF) is a potent vascular permeability factor and a mediator of brain edema. To assess the role of VEGF in eosinophilic meningitis, VEGF was measured in the cerebrospinal fluid (CSF) and blood of 9 patients with eosinophilic meningitis in a cohort study. VEGF CSF was detected in 8 (90%) of 9 eosinophilic meningitis patients (range, 45–2190 pg/mL) at presentation. The mean VEGF CSF at presentation, one week and two weeks after admission was 568 pg/mL, 751 pg/mL and 1031 pg/mL, respectively. There was an association between VEGFCSF, CSF protein, white cell count, and eosinophil counts. The VEGFSERUM fluctuated during the 6-month follow up period. These results indicate that VEGF may be associated with blood-brain barrier disruption in patients with eosinophilic meningitis. Our another study is designed to evaluate the possibility of blood-brain barrier (BBB) dysfunction caused by matrix metalloproteinase-9 (MMP-9) and its regulation by tissue inhibitors of metalloproteinase (TIMPs) in patients with eosinophilic meningitis caused by the infection with A. cantonensis, 40 patients and 28 controls were included in this study. Concentrations of MMP-2, MMP-9, TIMP-1 and CSF/serum albumin ratio (QAlb values) are significantly increased in patients than those in controls, however, TIMP-4 is significantly lower in patients. Contrarily to MMP-2, proteolytic activity of MMP-9 detected by gelatin zymography was only observed in patients with eosinophilic meningitis. It was observed that the higher the antibody levels specific to A. cantonensis in CSF of patients with eosinophilic meningitis, the higher the QAlb values and MMP-9 concentrations in CSF of patients. Furthermore, the increase in QAlb value was significantly correlated with the increase of MMP-9 in patients. In parallel with CSF MMP-9, patients also showed an increase of CSF white blood cell counts. Gradual decrease levels of QAlb, MMP-9 and TIMP-1 and increase levels of TIMP-4 were observed in 6 patients during recovery from eosinophilic meningitis. These results propose that the source of MMP-9 in CSF of patients with eosinophilic meningitis was probably associated with leukocytes migrating from peripheral blood to CSF. The activity of MMP-9 in CSF of patients could not completely inhibited because of the decrease of TIMP-4 which had the chance to cause BBB dysfunction as proven by the higher QAlb values found in patients. In conclusion, BBB dysfunction may occur in patients with eosinophilic meningitis due to the infection of A. cantonensis because patients show significantly higher QAlb which significantly correlates with the concentrations of MMP-9 in CSF of patients. And also VEGF may be associated with blood-brain barrier disruption in patients with eosinophilic meningitis. The increase of MMP-9 in CSF of patients probably derives from leukocytes because they appeared a large number in CSF. Although TIMP-1 increased in the CSF of patients to neutralize the MMP-9, the proteolytic activity of MMP-9 still showed high levels. The reason why TIMP-4 in CSF of patients suddenly steeply dropped in the acute phase of eosinophilic meningitis and its role in BBB dysfunction of this parasitic disease should be clarified in further studies.
45
Chang, Shih Hsin, and 張世欣. "Identification of stage-specific Angiostrongylus cantonensis proteases by gene expression profiling." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/80884027010498345432.
Full text
46
柳源源. "Studies on rat Angiotrongylus cantonensis infection, experimental infection of Angiostrongylus cantonensis on canine and blood chemistry during infection." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/38630559610585760962.
Full textAbstract:
碩士國立中興大學
獸醫學研究所
81
In order to understand the condition of dogs after the infection of Angiostrongylus cantonensis, this experiment was performed. In this experiment dogs were divided into two groups , dogs of the first group were infected with 500 3rd stage larvae of Angiostrongylus cantonensis each. And the dogs of the second group were given ivermectin (0.05mg/kg, IM)every week for four weeks, rested for two weeks, then were infected with the same amount of 3rd stage larvae as the first group simultaneously with the first group. Blood was obtained from dogs of both group. and various tests were carried out to moniter the changes of blood CK, LDH, TP level and the antibody titer during the infection. Tests results of the first gruop were as followed ; the LDH level began to increase during the 3rd week postinfection (pi) and reach the top during the 5th and 6th week pi, then started to decrease, There were no notable changed of blood CK level . Total protein started to increase right after the infection, reached the hightest level during the 4th, 5th and 6th week, and dropped to the level.before the infection during the 8th week pi. number of WBC also started to increase right after the infection adn were the hightest during the 3rd week pi. Antibody titer were raised during the 3rd week pi, reached the top during the 4-6th week, then gradually started to decrease. Dogs of the second group were still protected by ivermectin which were not completely metablized and were not infected. The test results of these dogs showed no significant differences as compared with the test results of the control group.
47
Ke-Min and 陳科銘. "Angiostrongylus cantonensis induced proteolytic cascade with blood–CNS barrier damage in mice." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/06863939072122870091.
Full textAbstract:
博士中山醫學大學
生化暨生物科技研究所
95
Angiostrongylosis is a neurological disorder caused by invasion of the central nervous system and induced the infiltration of eosinophils into the subarachnoid space. The pathological changes are eosinophilic meningitis and eosinophilic meningoencephalitis by developing larvae of Angiostrongylus cantonensis. The activities of tissue-type plasminogen activator (tPA), urokinase-type activator (uPA) and MMP-9 in cerebrospinal fluid (CSF) were significantly increased in Angiostrongylus cantonensis infected mice with eosinophilic meningitis compared to that in uninfected mice. The cerebrospinal-like fluid eosinophils significantly correlated with total protein and albumin. The uPA, tPA, and MMP-9 activities were detected by substrate zymography. MMP-9 and tissue inhibitors of metalloproteinases -1(TIMP-1) mRNA and protein levels were examined by RT-PCR and immunoblots, respectively. In cerebrospinal-like fluid, the expression of eosinophils, total protein and albumin contents correlated significantly with uPA, tPA, and MMP-9 intensity by Spearman’s ranking correlation test. MMP-9/TIMP-1 imbalance in angiostrongylosis may be associated with eosinophilic meningitis. Immunohistochemistry demonstrated that MMP-9 and TIMP-1 localized in eosinophils and macrophages. In addition, administration of MMPs inhibitor, GM6001, in infected mice, MMP-9 enzyme activity, albumin concentrations and eosinophils declined significantly from their preinjection highs. These results suggested that PAs and MMP-9 proteolytic cascade and MMP-9/TIMP-1 imbalance may be associated with blood-CNS barrier disruption and CSF total protein, albumin and eosinophil might be an indicator of the blood-CNS barrier damage in eosinophilic meningitis caused by Angiostrongylus cantonensis.
48
Tsai, Yueh-Ting, and 蔡岳廷. "Study on T helper cell immune response induced by Angiostrongylus cantonensis antigens." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/26897903182153024921.
Full textAbstract:
碩士國立臺灣大學
微生物學研究所
93
The first human case of angiostrongylosis was reported from Taiwan in 1945. At present, Angiostrongylus cantonensis is the main cause of eosinophilic meningoencephalitis in Taiwan. In this study, serum obtained from rabbit hyperinfection with A. cantonensis was used to screen the cDNA library constructed in our laboratory. Two selected clones (AC 4 and AC 5) which showed strong positive reaction with anti-A. cantonensis antibody were selected for further study. Sequenceing analysis revealed that the cDNA fragment for AC 4 is 483bp (160 a.a) length. BLAST search revealed that it exbits 70% homology to apyrase from Caenorhabditis elegans. The other cDNA fragment for AC 5 is 507bp (168 a.a) long. BLAST search revealed that it exbits 98% homology to Rab2 family (Ras associated protein) family from C.elegans. Cytokine production in spleen cell from normal and A. cantonensis- infected mice in culture supernatants with A. cantonensis recombinant protein AC 4 or AC 5 were assessed by the commercial ELISA kit. The results revealed that the amount of cytokines IL-4, IL-5 and IL-13 were not significantly elevated after stimulation with AC 4 or AC 5 . But the production of IFN-γ have significantly increased when compare with control. The increase in IFN-γ suggest that AC 4 and AC 5 may help stimulating Th1 cytokine production.
49
Twu, Jun-Jhon, and 涂志宗. "Gelatinase B (Matrix Metalloprotease-9) in brain of Angiostrongylus cantonensis infected mice." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/34466089592242190928.
Full textAbstract:
碩士中山醫學院
生物化學研究所
87
Matrix metalloproteinases(MMPs) are a group of extracellular matrix (ECM) degrading enzyme, responsible for the degradation of interstitial connective tissue and basement membrane, during wound healing and in other inflammatory states. The mechanism to brain injury, little is known about the ECM-degrading MMPs that in Angiostrongylus cantonensis infection. Thus, it was of interest to determine MMPs in the Angiostrongylus cantonensis infected mice brains tissue, we use substrate gelatine zymography to detected brain tissue homogensate express of a gelatinase(GLase) activity. In this study, we demonstrated the presence of gelatinolytic activity in the brain of Angiostrongylus cantonensis infected ICR strain mice. This protease M.W. about 94kDa, We identified this gelatinolytic activity as gelatinase(type IV collagenase), belonging to family of the matrix metalloproteinases(MMPs), based on the molecular weight and the inhibition by protease inhibitors. The 72 kDa form was present in all samples, even in those derived from non infection mice, whereas the 94 kDa form was present only in samples from mice infected with Angiostrongylus cantonensis. The 94-kDa GLase activity was Ca++ dependent, showed maximal activity in the pH 6-8, their activity was inhibited in the presence of 1,10-phenanthroline, and their proenzymes were cleaved and activated after incubation with organomercurial compounds (APMA) and trypsin. When immunoblots were performed using specific antisera against the 94-kDa gelatinase B (MMP-9) with brain tissue homogensate, a 94-kDa immunopositive band was observed. Thus, it indicated that the 94-kDa GLase is gelatinase B(MMP-9). Gelatinase production within the CNS by glia or infiltrating inflammatory cells may constitute an important pathogenic mechanism for both the distruption of the blood-brain barrier and the destruction of myelin, as observed in several neuroinflammatory disorders. This would be the direction for further research.
50
WANG, LIAN-CHENG, and 王蓮成. "EXPERIMENTAL INFECTIONS OF ANGIOSTRONGYLUS CANTONENSIS AND THEIR IMMUNE RESPONSES IN LABORATORY HOSTS." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/05553581856999574076.
Full text