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Journal articles on the topic 'Analisi in vitro'

Author: Grafiati

Published: 9 March 2023

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1

Marchetti, M., A. Mello, C. Nosenzo, P. Bonfante, and A. Fontana. "Sintesi in Vitro TraTuber MaculatumVitt. eCistus IncanusL.: Controllo Morfologico ed Analisi Molecolare." Giornale botanico italiano 130, no. 1 (January 1996): 285. http://dx.doi.org/10.1080/11263509609439543.

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Krishaditersanto, R., T. O. D. Dato, and A. E. Manu. "Perubahan Kandungan Nutrisi dan Kecernaan in vitro Serbuk Gergaji Hasil Biokonversi Menggunakan Inokulum Jamur Tiram Putih (Pleurotus ostreatus) dengan Level Suplementasi Urea yang Berbeda." Jurnal Sain Peternakan Indonesia 12, no. 2 (June 26, 2017): 124–32. http://dx.doi.org/10.31186/jspi.id.12.2.124-132.

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Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi urea pada subtrat terhadap perubahan kandungan nutrisi dan kecernaaan in vitro serbuk gergaji hasil biokonversi menggunakan inokulum jamur tiram putih (Pleurotus ostreatus) dengan lama inkubasi 40 hari. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) faktor tunggal dengan 4 level suplementasi urea dan 3 ulangan. Paramenter yang diamati adalah persentase perubahan kandungan bahan kering, bahan organik, serat kasar, protein kasar, serta lemak kasar; dan perubahan kecernaan in vitro bahan kering serta bahan organik. Analisa data menggunakan analisi variansi menunjukan bahwa suplementasi urea berpengaruh nyata (P<0,05) terhadap persentase penurunan bahan kering, bahan organik, dan serat kasar dimana persentase penurunan terrtinggi pada suplementasi urea sebanyak 1%. Persentase peningkatan kandungan protein kasar dan lemak kasar secara nyata berbeda (P<0,05) pada tiap perlakuan, persentase peningkatan teringgi pada level suplementasi urea 1%. Suplementasi urea juga berpengaruh secara nyata (P<0,05) terhadap persentase peningkatan kecernaan in vitro bahan kering dan bahan organik dimana peningkatan paling tinggi pada level suplementasi urea 1% dalam subtrat.Kata kunci: jamur tiram putih, serbuk gergaji, urea

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3

Incardona, M., M. Ricci, L. Mencacci, A. Genovesi, and U. Covani. "Il mantenimento della riabilitazione protesica: analisi morfologica in vitro con differenti sistemi di decontaminazione." Prevenzione & Assistenza Dentale 37, no. 3 (September 2011): 81–86. http://dx.doi.org/10.1016/j.pad.2011.03.003.

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4

Silaban, Ayu, M. Afdal, and Darlis Darlis. "Penggunaan Feses Kerbau Dan Sapi Sebagai Inokulum Pengganti Cairan Rumen Dalam Mendegradasi NDF,ADF Dan Hemiselulosa Pakan Ternak Secara Metoda In Vitro." Jurnal Ilmiah Ilmu-Ilmu Peternakan 25, no. 1 (January 6, 2023): 34–39. http://dx.doi.org/10.22437/jiiip.v25i1.23246.

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Penelitian ini bertujuan untuk mengevaluasi penggunaan feses kerbau dan feses sapi sebagai in-okulum dalam penggantian cairan rumen dalam mendegradasi Neutral detergent fibre (NDF), acid detergent fibre (ADF) dan hemiselulosa secara In vitro. Penelitian ini dilaksanakan selama 1 bulan di Laboratorium Nutrisi dan Makanan Ternak Fakultas Peternakan Universitas Jambi. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 3 perla-kuan dan 6 ulangan. Perlakuan adalah P0 = Cairan rumen (kontrol) , P1 = Cairan feses kerbau + Molases 5%, dan P2 = Cairan feses sapi + Molases 5%. Peubah yang diamati adalah degradasi Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF) dan Hemiselulosa. Data diolah secara statistik dengan analisis ragam ANOVA (Analisi of Variance) dan jika terdapat pengaruh perla-kuan yang nyata dilanjutkan uji Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa per-lakuan berpengaruh nyata (P<0.05) terhadap degradasi NDF, ADF dan Hemiselulosa. Kesimpulan dari penelitian ini yaitu Penggunaan inokulum feses kerbau belum mampu menyamai cairan ru-men sebagai inokulum namun inokulum feses sapi dapat digunakan sebagai pengganti cairan rumen dalam mendegradasi NDF, ADF dan hemiselulosa karena terlihat bahwa perlakuan inoku-lum feses kerbau dan sapi lebih tinggi dibandingkan cairan rumen dalam mendegradasi NDF, ADF dan Hemiselulosa.

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Silaban, Ayu Lestari, M. Afdal, and Darlis Darlis. "Penggunaan Feses Kerbau dan Sapi Sebagai Inokolum Pengganti Cairan Rumen Dalam Mendegradasi NDF, ADF Dan Hemiselulosa Pakan Ternak Secara Metoda In-Vitro." Jurnal Ilmiah Ilmu-Ilmu Peternakan 25, no. 1 (July 1, 2022): 34–39. http://dx.doi.org/10.22437/jiiip.v25i1.12681.

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Penelitian ini bertujuan untuk mengevaluasi penggunaan feses kerbau dan feses sapi sebagai inokulum dalam penggantian cairan rumen dalam mendegradasi Neutral detergent fibre (NDF), acid detergent fibre (ADF) dan hemiselulosa secara In vitro. Penelitian ini dilaksanakan selama 1 bulan di Laboratorium Nutrisi dan Makanan Ternak Fakultas Peternakan Universitas Jambi. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 6 ulangan. Perlakuan adalah P0 = Cairan rumen (kontrol) , P1 = Cairan feses kerbau + Molases 5%, dan P2 = Cairan feses sapi + Molases 5%. Peubah yang diamati adalah degradasi Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF) dan Hemiselulosa. Data diolah secara statistik dengan analisis ragam ANOVA (Analisi of Variance) dan jika terdapat pengaruh perlakuan yang nyata dilanjutkan uji Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh nyata (P<0.05) terhadap degradasi NDF, ADF dan Hemiselulosa. Kesimpulan dari penelitian ini yaitu Penggunaan inokulum feses kerbau belum mampu menyamai cairan rumen sebagai inokulum namun inokulum feses sapi dapat digunakan sebagai pengganti cairan rumen dalam mendegradasi NDF, ADF dan hemiselulosa karena terlihat bahwa perlakuan inokulum feses kerbau dan sapi lebih tinggi dibandingkan cairan rumen dalam mendegradasi NDF, ADF dan Hemiselulosa.

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6

Serra, Angelo. "La sperimentazione sull'embrione umano: una nuova esigenza della scienza e della medicina?" Medicina e Morale 42, no. 1 (February 28, 1993): 97–116. http://dx.doi.org/10.4081/mem.1993.1072.

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Le tecniche di fecondazione artificiale - la cui finalità prima doveva essere il superamento della sterilità di coppia- sono divenute in realtà occasione privilegiata per ottenere "materiale umano" su cui sperimentare. Gli embrioni rimasti in soprannumero o ottenuti con fecondazioni in vitro apposite, possono essere utilizzati in studi di biologia cellulare, di genetica molecolare, di citogenetica, e biochimici. In questo articolo, l'Autore, dopo una accurata analisi della situazione attuale, valuta se esista o meno l'esigenza di utilizzare gli embrioni umani nella sperimentazione, tenendo presente che, in quanto individui umani fin dalla fecondazione, essi esigono lo stesso rispetto dovuto a chi è già nato.

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Spagnolo, Antonio G. "Comitati di bioetica in tema di procreazione artificiale." Medicina e Morale 42, no. 1 (February 28, 1993): 205–30. http://dx.doi.org/10.4081/mem.1993.1077.

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Il dibattito pubblico relativo agli aspetti etici delle nuove tecnologie riproduttive è stato iniziato dagli stessi scienziati coinvolti nelle ricerche in questo campo. Successivamente, sin dalla metà degli anni '70 il ricorso a comitati o commissioni etiche è emerso come una modalità estremamente importante di analisi dei problemi in questo campo anche al fine delle successive decisioni di politica pubblica. L'autore fa una revisione dei pareri di Comitati etici nazionali nel campo delle tecnologie riproduttive considerando tre punti principali: a) i problemi dell'applicazione clinica della fecondazione in vitro; b) il problema della maternità surrogata; c) il problema della ricerca sull'embrione umano. Egli considera, quindi, alcune linee-guida relative al funzionamento dei Comitati Etici all'interno dei servizi di procreazione assistita. Infine, auspica che vengano definite da ogni comitato alcune procedure operative standard pubblicamente accettate che impegnino il comitato stesso a fornire il loro parere secondo alcuni criteri razionali.

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Barbanti-Brodano, G., S. Boriani, C. Di Bona, M. Tognon, and C. Morelli. "Le “cage” in carbonio (CFRP) favoriscono una maggiore proliferazione e una migliore distribuzione cellulare di quelle in polietere-etereketone (PEEK). Analisi mediante un modello innovativo per lo studio in vitro dei biomateriali." LO SCALPELLO-OTODI Educational 21, no. 2 (December 2007): 84–85. http://dx.doi.org/10.1007/s11639-007-0070-3.

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9

Santa Cruz, Francisco Vicente, Norma Fariña, Alejandra Leticia Walder, María del Carmen Rolón, Alicia Pereira, and Esteban Antonio Ferro. "In vitro sensitivity of Escherichia coli to fosfomicyn in urine cultures in a private healthcare service in Asunción." Anales de la Facultad de Ciencias Médicas (Asunción) 54, no. 2 (August 30, 2021): 61–70. http://dx.doi.org/10.18004/anales/2021.054.02.61.

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Anugrah, Devi, Susilo Susilo, and Hilman Faruq. "BIOINFORMATIK DAN ANALISIS GENETIK MIKROPROPAGASI Cibotium barometz." Bioma : Jurnal Ilmiah Biologi 9, no. 1 (May 9, 2020): 29–40. http://dx.doi.org/10.26877/bioma.v9i1.6032.

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Kultur in vitro sangat penting untuk pengembangan kultivar Simpei (Cibotium barometz) yang jumlahnya semakin langka. Penggunaan nitrogen dapat berpengaruh terhadap keberlanjutan sifat pemuliaan. Penelitian ini bertujuan untuk lebih menginvestifigasis pengaruh pemberian variasi konsentrasi nitrogen (KNO3) terhadap keragaman genetic plantet Cibotium barometz yang dihasilkan dari kultur in vitro. Tunas tunas dikumpulkan dan dikultur pada medium Murashige dan Skoog (MS) dengan konsentrasi nitrogen (KNO3) yang berbeda untuk pembentukan kultur in vitro. Dalam penelitian ini, analisis RAPD diterapkan untuk mengidentifikasi 4 planlet Cibotium barometz. Lima primer RAPD disusun untuk analisis ini. Hasil penelitian kami menunjukkan bahwa Polimorfisme pola pita DNA yang dihasilkan dari 5 primer RAPD menunjukkan keberagaman yang sangat tinggi hingga mencapai 100%. Hasil analisis klustering pola pita RAPD menggunakan metode UPGMA pada koefisien kemiripan 0.68 dan analisis komponen utama mampu dibedakan dengan tegas menjadi 3 kelompok. Kata Kunci : ; Cibotium barometz, Keragaman Genetik, Micropropagation, Nitrogen, PCR-RAPD

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11

Bukke, Suman, Venkataswamy Mallepogu, and ThyagaRaju Kedam. "Phytochemical Analysis, In-Vitro Antioxidant Activity and Proximate Analysis on Rhinacanthus Nasutus(L) Kurz Leaf." Indian Journal of Applied Research 3, no. 5 (October 1, 2011): 32–35. http://dx.doi.org/10.15373/2249555x/may2013/8.

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12

Braun, O., C. Contino, M. H. Hengé-Napoli, E. Ansoborlo, and B. Pucci. "Development of an in vitro test for screening of chelators of uranium." Analusis 27, no. 1 (January 1999): 65–68. http://dx.doi.org/10.1051/analusis:1999108.

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13

Stojanović, Sanja, Ivana Damnjanović, Jelena Najdanović, Tanja Džopalić, and Stevo Najman. "In vitro analysis of the biological activity of royal jelly on different cell lines." Hrana i ishrana 62, no. 2 (2021): 1–6. http://dx.doi.org/10.5937/hraish2102001s.

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Royal jelly is a bee product that is traditionally used as a dietary supplement but also as a potential remedy. Although it is widely used, experimental data that supports its therapeutic potential is lacking. Also, many studies have examined individual fractions and isolated substances from royal jelly, but there are very few studies that examine the native form of royal jelly, the one used in the daily diet. Our aim was to examine the effects of royal jelly, in the form of lyophilized powder product commercially available on the market, on the viability and proliferation of different cell lines in vitro. Our results showed that examined royal jelly product did not influence the cell viability of examined cell lines in examined concentrations while acted anti-proliferative in concentration-dependent manner on HeLa, cancer cell line but not on MDCK, non-cancer cell line. We can conclude that royal jelly contains some compounds that could exert certain activity towards cancer cells indicating its potential to which further studies should be directed.

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NISHAT, Sumaira, Fazli Rabbi AWAN, and Sadia Zafar BAJWA. "Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics." Analytical Sciences 35, no. 2 (February 10, 2019): 123–31. http://dx.doi.org/10.2116/analsci.18r001.

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Nurcahyani, Endang, Desti Deria Rahmadani, Sri Wahyuningsih, and Mahfut Mahfut. "ANALISIS KADAR KLOROFIL PADA BUNCIS (Phaseolus vulgaris L.) TERINDUKSI INDOLE ACETIC ACID (IAA) SECARA IN VITRO." ANALIT:ANALYTICAL AND ENVIRONMENTAL CHEMISTRY 5, no. 01 (April 30, 2020): 15–23. http://dx.doi.org/10.23960/aec.v5.i1.2020.p15-23.

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Buncis (Phaseolus vulgaris L.) adalah salah satu komoditas pangan yang memiliki sumber protein dan aman dikonsumsi.Seiring dengan berjalannya waktu dan perubahan iklim yang tidak menentu, para petani sayuran termasuk Buncis kesulitan dalam memproduksi hasil pertanian dengan baik, sehingga menyebabkan produksi tanaman buncis menurun. Indole Acetic Acid (IAA) merupakan hormon auksin yang terdapat pada ujung akar, batang dan pembentukan bunga. Hormon auksin IAA dapat memicu percepatan pertumbuhan pada akar, batang dan perkembangan bunga. Penelitian ini bertujuan untuk mengetahui efek pemberian IAA pada jumlah planlet hidup dan kadar klorofil a, b dan total planlet buncis (Phaseolus vulgaris L.) secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan variasi konsentrasi Indole Acetic Acid yang terdiri dari 4 taraf perlakuan yaitu 0 ppm, 0,5 ppm, 1 ppm, dan 1,5 ppm. Masing masing konsentrasi dilakukan 5 kali pengulangan dan setiap pengulangan terdiri dari 3 eksplan biji buncis dalam setiap botol kultur. Data dianalisis dengan menggunakan Uji homogenitas dan Uji Lanjut Beda Nyata Terkecil (BNT) pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa persentase planlet hidup menunjukkan 100% hidup dan pemberian IAA memberikan pengaruh terhadap kadar klorofil a dan total secara in vitro.

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Sabbieti, M. G., L. Marchetti, R. Curini, G. Menghi, A. Roda, M. V. Russo, C. Nugnes, and S. Materazzi. "Evidence of butyl benzyl phtalate induced modifications in a model system developed in vitro." Analusis 28, no. 9 (November 2000): 843–46. http://dx.doi.org/10.1051/analusis:2000153.

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HARADA, Masaaki, Masashi SHIBATA, Takehiko KITAMORI, and Tsuguo SAWADA. "Sub-Attomole Molecule Detection in a Single Biological Cell in-vitro by Thermal Lens Microscopy." Analytical Sciences 15, no. 7 (1999): 647–50. http://dx.doi.org/10.2116/analsci.15.647.

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ÇİÇEK, Betül, Ali TAGHİZADEHGHALEHJOUGHİ, and Ahmet HACIMÜFTÜOĞLU. "Glutamat Kaynaklı Nörotoksisite Üzerinde Pycnogenol'un Koruyucu Etkisinin Araştırılması: İn Vitro Analizi." Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 14, no. 2 (August 31, 2021): 864–73. http://dx.doi.org/10.18185/erzifbed.877837.

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19

Nasiu, Firman. "Suplemetasi Vitamin E dalam Cairan Rumen In Vitro: Analisis Parameter Fermentasi." Buletin Peternakan 40, no. 2 (June 30, 2016): 138. http://dx.doi.org/10.21059/buletinpeternak.v40i2.10752.

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KIM, Sung Bae, Ryo NISHIHARA, Rika FUJII, Ramasamy PAULMURUGAN, Daniel CITTERIO, and Koji SUZUKI. "In vitro Determination of Rapamycin-triggered FKBP–FRB Interactions Using a Molecular Tension Probe." Analytical Sciences 35, no. 1 (January 10, 2019): 71–78. http://dx.doi.org/10.2116/analsci.18sdp08.

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Irfan Ullah, Irfan Ullah, Jamshaid Ali Khan Jamshaid Ali Khan, Achyut Adhikari Achyut Adhikari, Peer Abdul Hannan Peer Abdul Hannan, Faisal Shakeel Muhammad Kifayatullah Faisal Shakeel Muhammad Kifayatullah, and Ajmal Khan and Shafiq Ur Rahman Ajmal Khan and Shafiq Ur Rahman. "GCMS Analysis and In-Vitro Activities of Monotheca buxifolia (Falc.) A. DC. Fruit." Journal of the chemical society of pakistan 41, no. 1 (2019): 143. http://dx.doi.org/10.52568/000709/jcsp/41.01.2019.

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The aim of the current study was to evaluate various fractions of Monotheca buxifolia fruit chemically using GC-MS and in vitro antibacterial, antifungal, and leishmanicidal potentials in order to provide scientific evidences for its folk uses. A variety of pharmacologically active compounds were identified including hexadecanoic acid, oleic acid, vaccinic acid, and pthanlic acid. All the tested samples were found fairly effective in antibacterial assay, P. aeruginosa was found more susceptible to ethyl acetate fraction with inhibition of 69.5 %, while B. subtilis was found completely resistant. Similarly the growth of M. canis and F. solani were moderately inhibited by M. buxifolia. The maximum (50%) antifungal effect was observed in case of ethyl acetate fraction against F. solani, While no leishmanicidal activity was found in the entire tested samples. Yet the use of this plant can be validated in the management of different bacterial and fungal infections.

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Purwoko, Devit, Teuku Tajuddin, Pramono, Rismayanti, Muhamad Farkhan Yanuar, Imastini Dinuriah, Hidayatul Arisah, and Dita Agisimanto. "ANALISIS HOMOGENITAS GENETIK KLON APEL (Malus spp.) HASIL PERBANYAKAN EX VITRO BERDASARKAN PENANDA SSR." Jurnal Bioteknologi & Biosains Indonesia (JBBI) 8, no. 1 (June 3, 2021): 57–67. http://dx.doi.org/10.29122/jbbi.v8i1.4029.

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Analysis of the Genetic Homogeneity of Apple (Malus spp.) Clones Propagated by Ex Vitro Culture Based on SSR Markers Propagation of apple plants (Malus spp.) vegetatively has the advantage of genetic homogeneity between clones and their parents. However, the possibility of cytological and off-type deviations persists during mass propagation. This study aims to analyze the genetic homogeneity of apple plants using SSR markers in ex vitro propagation techniques. Morphological observations between the parental plant and the ex vitro propagation derived clones and also among themselves did not show any differences. To analyze the genetic heterogeneity of these plants, 15 SSR primers were screened on 29 propagation clones of apple var. Anna and Manalagi. Genetic characters were scored for group analysis using Darwin 6.05. Eight SSR primers (IPA 2, IPA 3, IPA 4, 5 PA, 6 PA, 12 PA, 13 PA, and 14 PA) produced 82 clear and easily visible bands in the size range of 90-365 bp. This study successfully detected the uniformity of all band patterns from the plant samples. The SSR markers could be used to analyze the genetic stability of the ex vitro propagation of apple clones. Perbanyakan tanaman apel (Malus spp.) secara vegetatif memiliki keunggulan homogenitas genetik antara klon dengan induknya. Namun kemungkinan terjadi penyimpangan sitologi dan off type tetap ada saat perbanyakan massal. Penelitian ini bertujuan untuk menguji homogenitas genetik tanaman apel menggunakan penanda SSR pada teknik perbanyakan ex vitro. Pengamatan secara morfologi antara tanaman induk dengan hasil perbanyakan secara ex vitro dan dengan sesamannya tidak menunjukkan perbedaan. Untuk menganalisis homogenitas genetik tanaman ini kami menyaring 15 primer SSR pada 29 klon perbanyakan tanaman apel varietas Anna dan Manalagi. Karakter genetik dihitung untuk analisis kelompok menggunakan Darwin 6.05. Sebanyak 8 primer SSR (IPA 2, IPA 3, IPA 4, 5 PA, 6 PA, 12 PA, 13 PA, dan 14 PA) menghasilkan 82 pita yang jelas dan mudah terlihat dalam kisaran ukuran 90-365 bp. Studi ini juga berhasil mendeteksi keseragaman pola pita semua sampel tanaman. Penanda SSR dapat digunakan untuk analisis stabilitas genetik perbanyakan ex vitro dari tanaman apel.

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LIN, Chia-Chun, Yu-Tse WU, Jiin-Cherng YEN, Chang-Jung CHIANG, Yang-Hwei TSUANG, and Tung-Hu TSAI. "In vitro and in vivo Methods to Measure the Ceftriaxone Distribution into the Rat Tail Intervertebral Disc." Analytical Sciences 26, no. 9 (2010): 979–82. http://dx.doi.org/10.2116/analsci.26.979.

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KANG, Sun Kil, Ran-A. JEONG, Sejin PARK, Taek Dong CHUNG, Sunmin PARK, and Hee Chan KIM. "In vitro and Short-term in vivo Characteristics of a Kel-F Thin Film Modified Glucose Sensor." Analytical Sciences 19, no. 11 (2003): 1481–86. http://dx.doi.org/10.2116/analsci.19.1481.

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Rathahao, E., A. Hillenweck, A. Paris, and L. Debrauwer. "Investigation of the in vitro metabolism of 17b-estradiol by LC-MS/MS using ESI and APC." Analusis 28, no. 4 (May 2000): 273–79. http://dx.doi.org/10.1051/analusis:2000280273.

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Sumadi, Sumadi, A. Subrata, and Sutrisno Sutrisno. "Produksi Protein Total dan Kecernaan Protein Daun Kelor Secara In Vitro." Jurnal Sain Peternakan Indonesia 12, no. 4 (December 25, 2017): 419–23. http://dx.doi.org/10.31186/jspi.id.12.4.419-423.

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Penelitian dilaksanakan di Laboratorium Ilmu Nutrisi dan Pakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang bulan Oktober-November 2016. Penelitian bertujuan untuk mengkaji produksi protein total dan kecernaan protein dari daun kelor secara in vitro. Penelitian dilakukan dalam tiga tahap yaitu persiapan, uji in vitro dan analisis. Materi yang digunakan yaitu cairan rumen kambing peranakan etawa (PE), daun kelor dan daun lamtoro yang digunakan sebagai pembanding uji T-test. Produksi protein total di analisis dengan metode Tilley dan Terry tahap 1, sedangkan kecernaan protein di analisis menggunakan metode Tilley dan Terry 2 tahap. Analisis protein menggunakan metode Kjeldhal 1883 menurut (AOAC, 2005). Hasil penelitian menunjukan bahwa jumlah produksi protein total daun kelor tidak berbeda (p?0,05) dibandingkan dengan daun lamtoro (377,60 mg/g vs 478,92 mg/g). Kecernaan protein daun kelor nyata lebih tinggi (p?0,05) dibandingkan dengan daun lamtoro (64,29% vs 50,74%). Simpulan dari hasil penelitian adalah daun kelor mempunyai kualitas yang baik dibandingkan daun lamtoro karena memiliki kecernaan yang tinggi pasca rumen.Kata kunci: kelor, in vitro, produksi protein total, protein

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Nurcahyani, Endang, Dian Alfiah, Sri Wahyuningsih, and Mahfut Mahfut. "ANALISIS KANDUNGAN KARBOHIDRAT PADA PLANLET BUNCIS (Phaseolus vulgaris L.) SECARA IN VITRO HASIL INDUKSI KALIUM DALAM CEKAMAN KEKERINGAN." ANALIT:ANALYTICAL AND ENVIRONMENTAL CHEMISTRY 5, no. 01 (April 30, 2020): 34–41. http://dx.doi.org/10.23960/aec.v5.i1.2020.p34-41.

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Buncis (Phaseolus vulgaris L.) merupakan tanaman semusim berbentuk perdu. Pada tahun 2017 produktivitas buncis mengalami penurunan karena minimnya lahan produksi. Kendala yang dihadapi dalam memproduksi buncis antara lain musim kemarau yang berkepanjangan. Kalium dapat membantu penyerapan unsur hara dan air pada tumbuhan. Poly Ethylene Glycol (PEG 6000) diberikan pada medium kultur jaringan untuk menstimulasi cekaman kekeringan. Penelitian ini menggunakan medium Murashige and Skoog (MS) dengan pemberian kalium. Penelitian ini bertujuan untuk mengetahui efek pemberian kalium pada planlet buncis yang tahan serta untuk mengetahui kandungan karbohidrat pada planlet buncis secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu konsentrasi kalium dengan 4 taraf perlakuan: 0%, 0,5%, 0,15%, dan 0,25% dengan 6 ulangan. Data dianalisis dengan menggunakan ANOVA pada taraf 5%. Hasil penelitian menunjukkan bahwa persentase planlet hidup menunjukkan 100% hidup dan pemberian kalium dan PEG 6000 belum memberikan pengaruh terhadap kadar karbohidrat terlarut total secara in vitro.

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DİKER, Nurettin, and Burcu DİKER. "Influence of Peri-Implant Bone Defect Type on Insertion Torque and Resonance Frequency Analysis: An in Vitro Study." Turkiye Klinikleri Journal of Dental Sciences 28, no. 4 (2022): 763–71. http://dx.doi.org/10.5336/dentalsci.2022-90409.

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CHIBA, Hiromi, Yukari DEGUCHI, Ena KANAZAWA, Jun KAWAI, Keiichiro NOZAWA, Atsushi SHOJI, and Masao SUGAWARA. "In Vitro Measurements of Extracellular L-Glutamate Level in Region CA3 of Mouse Hippocampal Slices under Chemical Stimulation." Analytical Sciences 26, no. 10 (2010): 1103–6. http://dx.doi.org/10.2116/analsci.26.1103.

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Widiyanti, Ni Luh putu manik, Sanusi Mulyadiharja, and I. Nyoman Sukarta. "ANALISIS EKSTRAK TUMBUHAN REMPAH SEBAGAI PRESERVATIVES MAKANAN TAHU DIUJI SECARA IN VITRO." JST (Jurnal Sains dan Teknologi) 5, no. 2 (January 28, 2017): 870. http://dx.doi.org/10.23887/jst-undiksha.v5i2.8979.

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AbstrakMakanan tahu merupakan makanan tradisional dan sebagai sumber protein yang bermutu tinggi yang banyak mengandung asam amino esensial dan terbukti menurunkan kadar Low Density Lipoprotein. Tahu mudah mengalami pembusukan oleh bakteri, karena kadar protein dan kadar air yang tinggi. Tetapi banyak pengusaha nakal dengan menambahkan formalin dan pewarna methyl yellow yang dilarang penggunaaanya dalam makanan menurut peraturan Menkes Nomor 1168/Menkes/PER/X/1999 untuk pengawet makanan tahu. Alternatifnya adalah tanaman rempah sebagai antibakteri dan dimanfaatkan sebagai bahan pengawet alami makanan antara lain tanaman Cymbopogon ciratus (sereh), Cucurma domestika (kunyit) dan Alpinia galanga (lengkuas) untuk menghambat pertumbuhan bakteri pada makanan tahu. Tahu yang berhasil dibuat masih belum memenuhi standar penilaian SNI 01-3142-1992 yaitu pada bagian rasa, yaitu sebanyak 50% panelis yang menyatakan rasa tahu sedikit asam. Uji organoleptik makanan tahu pada hari pertama perendaman dengan preservatives alami, sebanyak 100% panelis menyatakan tahu yang direndam dengan ke-3 preservative alami beraroma dan berrasa harum preservatives alami (sereh, kunyit dan lengkuas). Karakteristik warna tahu, 100% panelis menyatakan putih bersih atau kekuningan bersih dan 100% panelis menyatakan penampakkan normal tidak berlendir dan berjamur. Terjadi penurunan kualitas tahu dari uji organoleptik pada hari ke-tiga dan ke-enam baik pada aroma, rasa, warna dan penampakan tahu. Gain Score menunjukkan bahwa tahu yang direndam dengan preservative alami sereh konsentrasi 20% yang menunjukkan peningkatan jumlah bakteri. Pada hari ke-3 dan ke-6, tahu yang direndam dengan konsentrasi 40% dan 60% menghambat pertumbuhan bakteri. Hasil Gain Score pada makanan tahu yang direndam dengan preservatives alami kunyit pada hari ke-1 dan ke-6 dengan konsentrasi 20%, 40% dan 60 % menunjukkan preservative kunyit pada semua konsentrasi tidak menghambat pertumbuhan bakteri yang hidup pada makanan tahu. Pada hari ke-3, hanya tahu yang direndam dengan konsentrasi 60% yang menghambat pertumbuhan bakteri. Gain Score menunjukkan makanan tahu yang direndam dengan preservatives alami lengkuas pada hari ke-1 hanya konsentrasi 40% yang menunjukkan penghambatan jumlah bakteri. Pada hari ke-3 dan ke-6, tidak ada konsentrasi preservatives lengkuas menghambat pertumbuhan bakteri. Jumlah SPC bakteri yang direndam dengan preservative sereh konsentrasi 40% berbeda bermakna dengan preservatives kunyit konsentrasi 20% dan lengkuas 20%. Jumlah SPC bakteri yang direndam dengan preservatives sereh konsentrasi 60% berbeda bermakna dengan preservatives kunyit konsentrasi 20%, lengkuas 20% dan lengkuas 40%. Jumlah SPC bakteri yang direndam dengan preservatives kunyit 20% berbeda bermakna dengan preservatives sereh 40% dan sereh 60%. Jumlah SPC bakteri yang direndam dengan preservative lengkuas 20% berbeda bermakna dengan preservatives sereh 40% dan sereh 60%. Jumlah SPC bakteri yang direndam dengan preservatives lengkuas 40% berbeda bermakna dengan preservatives sereh 60% dengan p<0,05. Zona hambatan oleh sereh konsentrasi 20% dan 40% berbeda bermakna dibandingkan dengan zona hambatan oleh kunyit 60% dan lengkuas 60% terhadap pertumbuhan bakteri. Zona hambatan oleh sereh konsentrasi 60% berbeda bermakna dibandingkan dengan zona hambatan oleh ketiga preservatives konsentrasi 20% dan 40% terhadap pertumbuhan bakteri. Zona hambatan oleh kunyit konsentrasi 20% berbeda bermakna dibandingkan dengan zona hambatan oleh ketiga preservatives konsentrasi 60% terhadap pertumbuhan bakteri. Zona hambatan oleh kunyit konsentrasi 40% berbeda bermakna dibandingkan dengan zona hambatan oleh ketiga preservatives konsentrasi 60% terhadap pertumbuhan bakteri. Zona hambatan oleh kunyit konsentrasi 60% berbeda bermakna dibandingkan dengan zona hambatan oleh ketiga preservatives konsentrasi 20% dan 40% terhadap pertumbuhan bakteri. Zona hambatan oleh lengkuas konsentrasi 20% dan 40% berbeda bermakna dibandingkan ketiga preservatives konsentrasi 60% terhadap pertumbuhan bakteri. Zona hambatan oleh lengkuas konsentrasi 60% berbeda bermakna dibandingkan dengan zona hambatan oleh ketiga preservatives konsentrasi 20% dan 40% terhadap pertumbuhan bakteri. Adanya zona hambatan disebabkan karena bahan aktif yang terdapat pada ketiga preservatives. Uji koefisien phenol menunjukkan bahwa ke- 3 preservatives alami yang diekstrak dengan hidrodestilasi dengan nilai sama atau kurang efektif dibandingkan phenol. Kata kunci : tanaman rempah, pengawet alami, makanan tahu, uji in vitro AbstractTofu is know as a traditional food and as a source of high quality protein that contains many essential amino acids and is proven to reduce levels of Low Density Lipoprotein. Tofu susceptible to decomposition by bacteria, because high of the protein content and water content. But many food entrepreneurs that naughty by adding formalin and methanyl yellow dye that banned its use in food regulation by Minister of Health Num. 1168/Menkes/PER/X/1999 for the food preservatives of tofu. The alternative is an antibacterial plants and used as a natural food preservatives including Cymbopogan ciratus (lemongrass), Cucurma domestica (turmeric) and Alpinia galanga (galangal) to inhibit bacterial growth in tofu. Tofu that has been created is still not meet the assessment standards of SNI 01-3142-1992 in aroma part, whereas 50% panelists who stated aroma tofu is slightly acidic. Organoleptic test of tofu in first day of soaked with natural preservatives, 100% panelists who stated tofu that soaked with three kinds natural preservatives with aroma and taste of Cymbopogon ciratus (lemongrass), Cucurma domestica (turmeric) and Alpinia galanga (galangal) extracts. The characteristic color of tofu, 100% panelists expressed that tofu with pure white color or pure yellowish and 100% panelists who stated normal of tofu in performance without slimy and moldy. In fact characteristic of tofu is decrease of quality result from organoleptic test on day three and six both in aroma, taste, color and performance of tofu. Gain score result show that tofu soaked with natural preservatives lemongrass concentration 20% that show increase bacteria in number. On third and six days, tofu soaked with 40% and 60% concentrations of lemongrass inhibit growth of bacteria. Result gain score indicates tofu that soaked with tumeric extract on first and sixth days soaked with 20%, 40% and 60% concentrations show all of tumeric extract concentrations didnt inhibit growth of bacteria. On the third day tofu that soaked with only 60% concentration capable to inhibit growth of bacteria. Result gain score indicates tofu that soaked with extract of galangal in first day, only concentration 40% show inhibit growth of bacteria. On third and sixth days, all of concentrations of galangal extract didnt capable inhibit growth of bacteria. Number of SPC bacteria in tofu soaked with preservatives lemongrass with concentration 40% was significantly different with tofu that soaked extract of 20% concentrations of turmeric and galangal. Number of SPC bacteria in tofu soaked with lemongrass concentration 60% was significantly different with 20% concentrations of both turmeric and galangal, and 40% concentration of galangal. Number SPC of bacteria in tofu soaked with 20% concentration of turmeric was significantly different with 40% and 60% concentrations of lemongrass. Number SPC of bacteria in tofu soaked with 20% concentration of galangal, was significantly different with 40% and 60% concentrations of lemongrass. Number SPC of bacteria in tofu soaked with 40% concentration of galangal was significantly different with 60% concentration of preservatives lemongrass with p<0,05. Inhibition zone by 20% and 40% concentration of lemongrass was significantly different compared with inhibition zone by both 60% concentrations of turmeric and galangal toward growth of bacteria. Inhibition zone by 60% concentration of lemongrass was significantly different compared with inhibition zone by three kinds of preservatives in 20% and 40% concentrations toward growth of bacteria. Inhibition zona by 20% concentration of tumeric was significantly different compared with inhibition zone by three kinds of preservatives in 60% concentration toward growth of bacteria. Inhibition zone by 40% concentration of tumeric was significantly different compared with inhibition zone by three kinds of preservatives in 60% concentration toward growth of bacteria. Inhibition zone by 60% concentration of tumeric was significantly different compared with inhibition zone by three kinds of preservatives in 20% and 40% concentrations toward growth of bacteria. Inhibition zone by 20% and 40% concentrations of galangal was significantly different compared with inhibition zone by three kinds of preservatives in 60% concentration toward growth of bacteria. Inhibition zone by 60% concentration of galangal was significantly different compared with inhibition zone by three kinds of preservatives in 20% and 40% concentrations toward growth of bacteria. Inhibiton zone was present in growth of bacteria caused by active substances in three kinds of natural preservatives. Coeffisient phenol test was done that show three kinds of natural preservatives was extracted by hydrodestillation with value equal or less effective than pure phenol. Key words : spices plants, natural preservatives, tofu, in vitro test.

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CHEN, Chia-Hsien, Chang-Jung CHIANG, Lien-Chen WU, Chih-Hong YANG, Yi-Jie KUO, and Tung-Hu TSAI. "In vitro Penetration and in vivo Distribution of Honokiol into the Intervertebral Disc in Rat." Analytical Sciences 31, no. 12 (2015): 1297–302. http://dx.doi.org/10.2116/analsci.31.1297.

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Hernández-Pimentel, Ma V., and S. Tavera-Martínez. "Análisis histológico del callo producido por hojas de Begonia x tuberhybrida cultivadas in vitro." Revista Chapingo Serie Horticultura II, no. 02 (February 1996): 171–76. http://dx.doi.org/10.5154/r.rchsh.1995.10.066.

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Thamin, Aswan, Chairulwan Umar, and Darussadah Paransa. "Analisis Pigmen dan Aktivitas Antibakteri In Vitro Pigmen Astaksantin Kepiting (Grapsus albolineatus Lamarck) Jantan." Jurnal Perikanan Universitas Gadjah Mada 8, no. 2 (July 15, 2006): 160. http://dx.doi.org/10.22146/jfs.133.

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Grapsus albolineatus is one of marine crustaceans which have carotenoid (astaxanthin) pigment. This research was conducted to analyze carotenoids (astaxanthin) extracted from G. albolineatus, and evaluate their in vitro antibacterial activity. The research was done in March-July 2002. Samples were collected from Manado Gulf, North Sulawesi. The result indicated that the carapace contained 4 carotenoids namely ß-caroten, ecinenon, astaxanthin diester, and astaxanthin monoester. In addition, the epidermis contained free astaxanthin. In vitro antibacterial activity test indicated that astaxanthin had low bacteriostatic activity against Psedomonas aeruginosa, Enterobacter cloacae, Staphylococcus aureus, and Proteus stuartii.

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Darmono, Gede Eko, Silva Triwidyaningtyas, Budiman Bela, Diah Iskandriati, Dondin Sajuthi, and Joko Pamungkas. "Analisis Infektivitas Simian-Human Immunodeficiency Virus pada Peripheral Blood Mononuclear Cell Macaca fascicularis dan M. nemestrina Secara In Vitro." Jurnal Veteriner 22, no. 1 (March 31, 2022): 16–26. http://dx.doi.org/10.19087/jveteriner.2022.23.1.16.

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Development of human immunodeficiency virus type 1 (HIV-1) vaccines and anti-retroviral treatmentis currently hindered by the lack of models representing prominent symptoms of HIV-1 infections seen in humans. Simian-human immunodeficiency virus (SHIV) was constructed to resolve the limitations of SIVmac model and has been used in nonhuman primate model ofviral infections, particularly infections by the close relatives of HIV-1. Macaca fascicularis and M. nemestrina are being developed as model HIV/AIDS, by using chimeric virus SHIV produced by replacing the nucleotide structure of cyclophilin A binding region, vif gene and nef of HIV-1 with cyclophilin A binding region, vif gene and nef from SIV. The research aims to study the model of HIV/AIDS on nonhuman primates PBMC in vitro using SHIV. In particular, the study aims to obtain information about the capability of SHIV replication in PBMC of M. fascicularis and M. nemestrina. Results showed a cytopathic effect (CPE) in the form of multinucleated giant cells and expression of p24 protein in PHA-stimulated PBMC cultures of M. fascicularis and M. nemestrina after SHIV infection. The conclusion of this study is that SHIV can infect PBMC M. fascicularis and M. nemestrina in vitro based on CPE and expression of p24 protein.

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Mehrotra, Nupur. "Effects of thermal treatments on ascorbic acid content of leafy vegetables: An in vitro analysis." Journal of Phytonanotechnology and Pharmaceutical Sciences 1, no. 1 (May 2021): 10–15. http://dx.doi.org/10.21276/jpps.2021.1.1.3.

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Topalović, Dijana, Lada Živković, Ninoslav Đelić, Vladan Bajić, and Biljana Spremo-Potparević. "Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay." Medicinski casopis 54, no. 3 (2020): 91–95. http://dx.doi.org/10.5937/mckg54-28798.

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Objective. Inosine 5'-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions.

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Manimaran, K., Arun Kumar, AvinashGandi D, and S. Sankaranarayanan. "Interaction of Human Dental Pulp Stem Cells and Ameloblastic Cell In-vitro- A Preclinical Analysis." Annals of Oral Health and Dental Research 2, no. 1 (January 17, 2018): A1–5. http://dx.doi.org/10.21276/aohdr.1831.

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Mira Humaira, Agus Purwito, Sudarsono, and Dewi Sukma. "Multiplikasi Tunas In Vitro Anggrek Phalaenopsis dan Analisis Keragaman Genetik dengan Marka SNAP." Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) 48, no. 1 (April 30, 2020): 59–67. http://dx.doi.org/10.24831/jai.v48i1.29149.

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Perbanyakan klonal anggrek melalui kultur in vitro dibutuhkan untuk memperoleh sejumlah besar propagul tanaman yang berkualitas. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh konsentrasi BAP terhadap kemampuan multiplikasi tunas dalam propagasi klonal beberapa anggrek hibrida Phalaenopsis dan menganalisis keragaman genetik menggunakan marka Pto-SNAP pada planlet klonal. Percobaan dilakukan secara terpisah pada 3 populasi tunas anggrek hibrida Phalaenopsis yaitu, tunas hasil perkecambahan biji dari Phal. amabilis x Phal. ‘KHM 421’ dengan 5 taraf perlakuan BAP (0.00, 11.09, 22.19, 33.29, 44.39 µM), tunas klon Phal. ‘Salu Spot’ x Phal. bellina ‘1102-38’ dan Phal. ‘Salu Spot’ x Phal. bellina ‘1102-44’ dengan 4 taraf perlakuan BAP (11.09, 22.19, 33.29, 44.39 µM). Hasil penelitian menunjukkan bahwa konsentrasi BAP terbaik untuk multiplikasi tunas pada tiga populasi yang diuji adalah 22.19 µM dengan jumlah tunas masing-masing 3.7, 4.7 dan 6.0 tunas pada 12 minggu setelah tanam (MST). Protocorm like bodies (PLBs) hanya dihasilkan pada populasi Phal. amabilis x Phal. ‘KHM 421’ dengan jumlah PLBs terbanyak perlakuan BAP 11.09 µM. Evaluasi keragaman genetik menggunakan 11 marker Pto-SNAP (single nucleotide amplified polymorphism) dilakukan pada planlet klonal Phal. ‘Salu Spot’ x Phal. bellina ‘1102-44’. Hasil analisis menunjukkan adanya variasi somaklonal dari planlet yang diuji perlakuan BAP 22.19 µM dengan keragaman 7.7% pada lokus Pto-241 dan perlakuan BAP 44.39 µM dengan persen keragaman 11.1% pada lokus Pto-424. Kata kunci: marka molekuler, propagasi klonal, Pto, sitokinin, variasi somaklonal

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Nurcahyani, Endang, Dian Pratiwi, Zulkifli Zulkifli, and Martha L. Lande. "ANALISIS KANDUNGAN KARBOHIDRAT TERLARUT TOTAL PLANLET BAYAM MERAH [Alternanthera amoena (Lem.) Voss] RESISTEN TERHADAP CEKAMAN GARAM (NaCl) SECARA IN VITRO." ANALIT:ANALYTICAL AND ENVIRONMENTAL CHEMISTRY 6, no. 02 (April 30, 2021): 114–21. http://dx.doi.org/10.23960/aec.v6.i2.2021.p114-121.

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Bayam merah [Alternanthera amoena (Lemaire) Voss.] banyak digemarioleh masyarakat karena kandungan nutrisinya yang bermanfaat seperti melancarkan peredaran darah, menurunkan tekanan darah tinggi dan kolesterol, sehingga produksi bayam merah harus lebih dioptimalkan. Saat ini banyak lahan pertanian yang terakumulasi oleh garam termasuk Indonesia. Salah satu alternatif yang dapat digunakan yaitu dengan penggunaan varietas bayam merah yang toleran terhadap cekaman garam. Penelitian ini bertujuan untuk mengetahui konsentrasi NaCl yang efektif terhadap pertumbuhan planlet bayam merah dan karakter ekspresi bayam merah dalam mengahadapi cekaman garam secara in vitro. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 taraf konsentrasi NaCl yaitu 0%; 0,25%; 0,50%; 0,75% dan 1% dengan 5 kali ulangan. Analisis data mengunakan one way ANOVA pada taraf 5% dan uji lanjut dengan uji Tukey pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa perlakuan NaCl berpengaruh pada karakter ekspresi planlet bayam merah seperti menurunnya tinggi tanaman dan peningkatan kandungan karbohidrat planlet bayam merah pada kondisi cekaman garam secara in vitro.

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LEE, Miran, Jeonghyeon PARK, Mi-sun LIM, Sook Jin SEONG, Joomi LEE, Jeong Ju SEO, Yong-Ki PARK, Hae Won LEE, and Young-Ran YOON. "High-throughput Screening of Inhibitory Effects of Bo-yang-hwan-o-tang on Human Cytochrome P450 Isoforms in vitro Using UPLC/MS/MS." Analytical Sciences 28, no. 12 (2012): 1197–201. http://dx.doi.org/10.2116/analsci.28.1197.

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Wahyudi, Anitria Urbaningrum, Hery Purnomo, and Badrus Zaman. "Analisis Kinerja Keuangan Itama Ranoraya Selaku Produsen Alat Kesehatan Non Elektromedik Periode 2018-2020." Widya Cipta: Jurnal Sekretari dan Manajemen 6, no. 2 (September 2, 2022): 128–37. http://dx.doi.org/10.31294/widyacipta.v6i2.12172.

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Program vaksinasi menyebabkan perluasan perangkat medis non-elektromedik, terutama dalam diagnostik In Vitro. Barang In Vitro, seperti Antigen Test Covid-19 (Panbio), meningkat sebesar 613,9 persen, memungkinkan perusahaan untuk memenuhi target penjualan 5-10 juta unit tahun ini. Akibatnya, tujuan dari penelitian ini adalah untuk memeriksa kinerja keuangan PT. Itama Ranoraya Tbk, yang sekarang menyaksikan peningkatan penjualan yang signifikan. Jenis penelitian deskriptif ini digunakan dalam penelitian ini. Menghitung dan menganalisis laporan keuangan PT. Itama Ranoraya Tbk pada tahun 2018-2020 berdasarkan rasio likuiditas, rasio solvabilitas, dan rasio profitabilitas menggunakan metodologi analisis data. Sebagai hasil dari temuan penelitian ini, kinerja keuangan perusahaan diklasifikasikan sebagai tidak likuid bila diukur dengan rasio likuiditas, bahkan jika rasio solvabilitas baik dalam rasio DAR, dan diklasifikasikan sebagai belum menguntungkan bila diukur dengan rasio profitabilitas.Kata Kunci: Kinerja, Keuangan, Kesehatan, Non Elektromedik

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KOMORI, Kikuo, Kenji MURAI, Shotaro MIYAJIMA, Takao FUJII, Shino MOHRI, Yoshiro ONO, and Yasuyuki SAKAI. "Development of an in vitro Batch-type Closed Gas Exposure Device with an Alveolar Epithelial Cell Line, A549, for Toxicity Evaluations of Gaseous Compounds." Analytical Sciences 24, no. 8 (2008): 957–62. http://dx.doi.org/10.2116/analsci.24.957.

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Kapitan, Origenes Boy, Laksmi Ambarsari, and Syamsul Falah. "In Vitro Antibakteri Ekstrak Etanol Puni (Zingiber zerumbet) Asal Pulau Timor." Savana Cendana 2, no. 02 (April 20, 2017): 29–32. http://dx.doi.org/10.32938/sc.v2i02.82.

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Puni (Zingiber zerumbet) merupakan tanaman yang tumbuh secara liar yang dimanfaatkan oleh penduduk di Pulau Timor dalam mengobati luka borok. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri esktrak tanaman dan mengetahui kandungan senyawa aktif pada ekstrak teraktif tanaman. Ekstraksi senyawa-senyawa aktif tanaman dilakukan menggunakan empat pelarut yakni pelarut n-heksan, etil asetat, etanol, dan air. Metode difusi agar digunakan dalam menguji aktivitas antibakteri ekstrak dan pencarian nilai KHTM untuk ekstrak teraktif. Analisis komponen senyawa aktif dilakukan menggunakan LC-MS. Ekstrak etanol diketahui memiliki kemampuan antibakteri terbaik dibanding ketiga ekstrak lainnya. Nilai KHTM ekstrak etanol terhadap bakteri S. aureus, B. subtilis, E. coli, dan P. aeruginosa berturut-turut sebesar 50, 100, 150, 250 mg mL-1. Hasil analisis LC-MS menunjukkan bahwa ekstrak etanol mengandung senyawa zerumbon dan gingerglikolipid B. ©2017 dipublikasikan oleh Savana Cendana.

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Aljwadi, Mohamed Salem Khalifa, and Sapna Smith La. "Analysis of Endonuclease activity in clinically and Environmental originated vibro species." Asian Pacific Journal of Health Sciences 6, no. 3 (September 2019): 56–61. http://dx.doi.org/10.21276/apjhs.2019.6.3.11.

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K. Riazunnisa, K. Riazunnisa, U. Adilakshmamma U. Adilakshmamma, and C. Habeeb khadri. "Phytochemical Analysis and In Vitro Antibacterial Activity of Soymida febrifuga (Roxb.) Juss. and Hemidesmus indicus (L.)." Indian Journal of Applied Research 3, no. 12 (October 1, 2011): 57–59. http://dx.doi.org/10.15373/2249555x/dec2013/14.

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Imran, Eisha, Faisal Moeen, Humayoon Satti, and Lubna Rahman. "Investigating the Cytotoxic and Anti-Bacterial Activity of Commercially Available Local Anesthetics: An In-Vitro Analysis." Journal of the Pakistan Dental Association 29, no. 04 (November 2, 2020): 185–92. http://dx.doi.org/10.25301/jpda.294.185.

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OBJECTIVE: To evaluate and compare the influence of three local anesthetic dental formulations manufactured in France (Septodont), Korea (Medicaine) and Pakistan (HD-Caine) in terms of cytotoxicity and anti-bacterial activities. METHODOLOGY: 90 commercially available local anesthetic cartridges of similar composition (2% lidocaine with epinephrine 100,000) viz. Septodont, Medicaine and HD-Caine were randomly collected from three different Pakistani cities and were assigned as Group S, Group M and Group H, respectively. The cartridges were further divided into three sub-groups each consisting of 10 cartridges to first evaluate cytotoxicity on Mesenchymal Stem Cells (MSCs) using a flow cytometer and secondly to investigate anti-bacterial activity by measuring zones of inhibition and through Broth Dilution Method against five bacterial strains. RESULTS: The results indicated that Septodont (94.5±0.1) and Medicaine (94.7±0.0) showed the highest viability percentage with no significant difference when the two were compared (P=0.6). HD-Caine (93.9±0.0) showed the least, being significantly (P<0.01) different from Septodont and Medicane. A statistically significant (P<0.05) difference was identified between the three study groups regarding the anti-bacterial activity. HD-Caine showed the highest anti-bacterial potential, followed by Medicaine and Septodont. CONCLUSION: Mild toxicity was observed by all the three groups in human MSCs, justifying their safe use in clinical practice. Additionally, Medicane and HD-Caine showed significant anti-bacterial activity indicating their possible use as sterile irrigants. KEYWORDS: Dental anesthesia, Lidocaine, Epinephrine, Antibacterial activity HOW TO CITE: Imran E, Moeen F, Satti H, Rahman L. Investigating the cytotoxic and anti-bacterial activity of commercially available local anesthetics: An in-vitro analysis. J Pak Dent Assoc 2020;29(4):185-192.

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Jafarian, Mohammad, Fatemeh Sadat Mirhashemi, and Naghmeh Emadi. "Finite element analysis of stress distribution around a dental implant with different amounts of bone loss: An in vitro study." Dental and Medical Problems 56, no. 1 (March 29, 2019): 27–32. http://dx.doi.org/10.17219/dmp/102710.

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Di Lullo, A. M., M. Scorza, F. Amato, M. Comegna, V. Raia, L. Maiuri, G. Ilardi, E. Cantone, G. Castaldo, and M. Iengo. "An ex vivo model contributing to the diagnosis and evaluation of new drugs in cystic fibrosis." Acta Otorhinolaryngologica Italica 37, no. 3 (June 2017): 207–13. http://dx.doi.org/10.14639/0392-100x-1328.

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La fibrosi cistica (FC) è una malattia autosomica recessiva causata da mutazioni nel gene CFTR (Cystic Fibrosis Transmembrane conductance Regulator). Finora sono state descritte circa 2000 mutazioni, ma per la maggior parte di esse è difficile definirne leffetto senza complesse procedure in vitro. Abbiamo effettuato il campionamento (mediante brushing), la cultura e lanalisi di cellule epiteliali nasali umane (HNEC) utilizzando una serie di tecniche che possono aiutare a testare leffetto delle mutazioni CFTR. Abbiamo eseguito 50 brushing da pazienti FC e controlli, e in 45 casi si è ottenuta una coltura positiva. Utilizzando cellule in coltura: i) abbiamo dimostrato lespressione ampiamente eterogenea del CFTR nei pazienti e nei controlli; ii) abbiamo definito leffetto di splicing di una mutazione sul gene CFTR; iii) abbiamo valutato lattività di gating di CFTR in pazienti portatori di differenti mutazioni; iv) abbiamo dimostrato che il butirrato migliora in modo significativo lespressione di CFTR. I dati provenienti dal nostro studio sperimentale dimostrano che luso del modello ex-vivo di cellule epiteliali nasali è un importante e valido strumento di ricerca e di diagnosi nella studio della FC e può anche essere mirato alla sperimentazione ed alla verifica di nuovi farmaci. In definitiva, in base ai nostri dati è possibile esprimere le seguenti conclusioni: 1) il prelievo delle cellule epiteliali nasali mediante brushing è applicabile senza alcuna anestesia ed è ben tollerato da tutti i pazienti affetti da FC (bambini e adulti), è scarsamente invasivo e facilmente ripetibile, è anche in grado di ottenere una sufficiente quantità di HNECs rappresentative, ben conservate, idonee allo studio della funzionalità di CFTR; 2) la conservazione delle cellule prelevate è possibile fino a 48 ore prima che si provveda allallestimento della coltura e ciò permette di avviare studi multicentrici con prelievi in ogni sede e quindi di ottenere una ampia numerosità campionaria; 3) la coltura di cellule epiteliali nasali può essere considerata un modello adatto a studiare leffetto molecolare di nuove mutazioni del gene CFTR e/o mutazioni specifiche di pazienti carriers dal significato incerto; 4) il modello ex-vivo delle HNECs consente inoltre di valutare, prima dellimpiego nelluomo, leffetto di farmaci (potenziatori e/o correttori) sulle cellule di pazienti portatori di mutazioni specifiche di CFTR; tali farmaci possono modulare lespressione genica del canale CFTR aprendo così nuove frontiere terapeutiche e migliori prospettive di vita per pazienti affetti da una patologia cronica come la Fibrosi Cistica; 5) la metodologia da noi istituita risulta essere idonea alla misura quantitativa, mediante fluorescenza, dellattività di gating del canale CFTR presente nelle membrane delle cellule epiteliali nasali prelevate da pazienti portatori di differenti genotipi; in tal modo è possibile individuare: a) pazienti FC portatori di 2 mutazioni gravi con unattività < 10% (in rapporto ai controlli -100%), b) soggetti FC portatori contemporaneamente di una mutazione grave e di una lieve con unattività tra 10-30%, c) i cosiddetti portatori carriers- eterozigoti - con unattività tra 40-70%. In conclusione la possibilità di misurare lattività del canale CFTR in HNECs fornisce un importante contributo alla diagnosi di FC, mediante individuazione di un cut-off diagnostico, ed anche alla previsione della gravità fenotipica della malattia; quindi quanto rilevabile dalla misura del suddetto canale permette di prospettare per il futuro la possibilità di valutare meglio i pazienti per i quali il test del sudore ha dato risultati ambigui (borderline o negativi). La metodica da noi sperimentata consente anche di monitorare i pazienti durante il trattamento farmacologico, valutando in tal modo i reali effetti delle nuove terapie.

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Karimah, Umul, Yogi Nur Anggowo, Syamsul Falah, and Suryani Suryani. "ISOLASI OLIGOSAKARIDA MADU LOKAL DAN ANALISIS AKTIVITAS PREBIOTIKNYA." Jurnal Gizi dan Pangan 6, no. 3 (November 8, 2011): 217. http://dx.doi.org/10.25182/jgp.2011.6.3.217-224.

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The objective of this study was to isolate oligosaccharides from local honey of Pulau Sumbawa and province of Kalimantan Timur and to analyze its prebiotic activity in vitro. Oligosaccharides were isolated with activated charcoal adsorption and ethanol treatment. Effectiveness of this method was evaluated by Thin Layer Chromatography and colorimetry. The isolates were then being a sample to prebiotic examination. Honey from Sumbawa forest has higher oligosaccharides content than honey from Kalimantan. Qualitative and quantitative assays showed that oligosaccharide isolation method was effective to concentrate the oligosaccharide with high degree of polymerization but ineffective for mono- and disaccharides removal. The highest percentages of hydrolysis of oligosaccharide from Sumbawa and Kalimantan by mimic gastric juice were 1.78% and 0.59 %. Whereas, the hydrolysis was higher with amylase, 11.87% for Sumbawa and 21.03% for Kalimantan honey oligosaccharides. Prebiotic activity was 0.32, and 0.09 for honey oligosaccharide from Sumbawa and Kalimantan respectively. Honey oligosaccharide from Sumbawa presented higher prebiotic activity than inulin as prebiotic reference (0.11). GC-MS chromatogram showed L. acidophilus cultured in media added with Sumbawa honey oligosaccharide produced lactic acid as the highest metabolite (48.01%).

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Nufus, Novita Hidayatun. "ANALISIS FITOKIMIA DAN UJI POTENSI EKSTRAK BUAH RENGGAK (Amomum Dealbatum) SEBAGAI PESTISIDA NABATI TERHADAP JAMUR Pyricularia Oryzae DAN BAKTERI Xanthomonas oryzae." Bioscientist : Jurnal Ilmiah Biologi 8, no. 1 (November 18, 2020): 115. http://dx.doi.org/10.33394/bjib.v8i1.2661.

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This study aims to determine the potency of Renggak (Amomum dealbatum) extract as bio pesticide against Pyricularia oryzae and Xanthomonas oryzae. Therefore an in vitro of antifungal and antibacterial activity of the Renggak ethanol extract were carried out against Pyricularia oeyzae and Xanthomonas oryzae. In vitro antifungal analysis were done using agar diffusing method by dissolving Renggak extract (1%, 5%, and 10%) into fungal medium and the growth of Pyricularia oryzae were observed. In vitro antibacterial analysis were complited using agar diffusing method with paper disk that contain Renggak extract (10%, 20%, and 30%) and antibiotic chloroamfenikol as positive control. The result suggest that Renggak fruit extract has antifungal and antibacterial activities. The treatment with 10% Renggak extract could inhibit the growth of Pyricularia oryzae to 100%, same with positive control (Fungicide Score-250). In vitro test for antibacterial activity showed that treatments with 20% and 30% Renggak extract gave inhibitory activities that similar with positive control. Renggak ethanol extract gave positive results on Flavonoid, Alkaloid, Steroid, Terpenoid, and Saponin tests. GC-MS assays showed that Renggak ethanol extract produces at least 10 organic compounds that was identified as 2-butanone, 4-methoxypheyl, Benzenepropanoic-aci, 4-hydroxyphenil, CAS, octadecanoid acid, stearic acid, palmiti acid, Benzenepropanoic acid, dan Farnesol isomer A, which have antimicrobial avtivities.

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