Academic literature on the topic 'Anaerobiosis'

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Journal articles on the topic "Anaerobiosis"

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de Sarrau, Benoît, Thierry Clavel, Caroline Clerté, Frédéric Carlin, Christian Giniès, and Christophe Nguyen-The. "Influence of Anaerobiosis and Low Temperature on Bacillus cereus Growth, Metabolism, and Membrane Properties." Applied and Environmental Microbiology 78, no. 6 (January 13, 2012): 1715–23. http://dx.doi.org/10.1128/aem.06410-11.

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ABSTRACTThe impact of simultaneous anaerobiosis and low temperature on growth parameters, metabolism, and membrane properties ofBacillus cereusATCC 14579 was studied. No growth was observed under anaerobiosis at 12°C. In bioreactors, growth rates and biomass production were drastically reduced by simultaneous anaerobiosis and low temperature (15°C). The two conditions had a synergistic effect on biomass reduction. In anaerobic cultures, fermentative metabolism was modified by low temperature, with a marked reduction in ethanol production leading to a lower ability to produce NAD+. Anaerobiosis reduced unsaturated fatty acids at both low optimal temperatures. In addition, simultaneous anaerobiosis and low temperatures markedly reduced levels of branched-chain fatty acids compared to all other conditions (accounting for 33% of total fatty acids against more 71% for low-temperature aerobiosis, optimal-temperature aerobiosis, and optimal-temperature anaerobiosis). This corresponded to high-melting-temperature lipids and to low-fluidity membranes, as indicated by differential scanning calorimetry, 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence anisotropy, and infrared spectroscopy. This is in contrast to requirements for cold adaptation. A link between modification in the synthesis of metabolites of fermentative metabolism and the reduction of branched-chain fatty acids at low temperature under anaerobiosis, through a modification of the oxidizing capacity, is assumed. This link may partly explain the impact of low temperature and anaerobiosis on membrane properties and growth performance.
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Andersen, P. C., J. M. Montano, and P. B. Lombard. "Root Anaerobiosis, Root Respiration, and Leaf Conductance of Peach, Willow, Quince, and Several Pear Species." HortScience 20, no. 2 (April 1985): 248–50. http://dx.doi.org/10.21273/hortsci.20.2.248.

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Abstract The effects of root anaerobiosis on root respiration and leaf conductance (kl) were determined in solution culture experiments. Respiration of feeder roots (<2 mm diameter) in air (21% O2) of Pyrus betulaefolia Bunge, Pyrus calleryana Decne, Pyrus communis L. (‘Old Home’ × ‘Farmingdale 97’) and Cydonia oblonga Mill. ‘Provence BA 29’ was reduced by no more than 50% after 21 days of anaerobiosis. In contrast, root respiration of Prunus persica (L.) Batsch ‘Lovell’ was reduced by 80% with anaerobiosis, whereas that of Salix discolor Muhl. increased. Reductions in kl with anaerobiosis generally were more pronounced than reduction in root respiration when measured in air. Respiration rates of aerobically or anaerobically treated pear roots were inhibited by 25% to 50% when incubated in 0.5% O2 compared to rates in air. More work is required in order to delineate the relationship of root respiration and kl with anaerobiosis.
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Champomier-Vergès, Marie-Christine, Anika Marceau, Thérèse Méra, and Monique Zagorec. "The pepR Gene of Lactobacillus sakei Is Positively Regulated by Anaerobiosis at the Transcriptional Level." Applied and Environmental Microbiology 68, no. 8 (August 2002): 3873–77. http://dx.doi.org/10.1128/aem.68.8.3873-3877.2002.

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ABSTRACT Lactobacillus sakei is a lactic acid bacterium belonging to the natural flora of meat products. It constitutes the main flora of vacuum-packed meat and is largely used in western Europe as a starter for the manufacturing of fermented sausages. This species is able to grow both under aerobiosis and anaerobiosis. In many technological processes involving it, oxygen is scarce. The aim of this study was to identify the major proteins affected by growth under anaerobiosis. Using two-dimensional electrophoresis, we showed that one spot was 10-fold overexpressed when cells were grown under anaerobiosis. By N-terminal sequencing it was identified as a peptidase (PepR), and the pepR gene was cloned. Northern analysis revealed that pepR was expressed as a single 1.27-kb transcript induced under anaerobiosis. A mutant was constructed by single crossover in the pepR gene, and its growth and survival were not affected by anaerobiosis.
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Volkland, H. P., H. Harms, O. Wanner, and A. J. B. Zehnder. "Corrosion protection by anaerobiosis." Water Science and Technology 44, no. 8 (October 1, 2001): 103–6. http://dx.doi.org/10.2166/wst.2001.0475.

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Biofilm-forming bacteria can protect mild (unalloyed) steel from corrosion. Mild steel coupons incubated with Rhodoccocus sp. strain C125 and Pseudomonas putida mt2 in an aerobic phosphate-buffered medium containing benzoate as carbon and energy source, underwent a surface reaction leading to the formation of a corrosion-inhibiting vivianite layer [Fe3(PO4)2]. Electrochemical potential (E) measurements allowed us to follow the buildup of the vivianite cover. The presence of sufficient metabolically active bacteria at the steel surface resulted in an E decrease to -510 mV, the potential of free iron, and a continuous release of ferrous iron. Part of the dissolved iron precipitated as vivianite in a compact layer of two to three microns in thickness. This layer prevented corrosion of mild steel for over two weeks, even in a highly corrosive medium. A concentration of 20 mM phosphate in the medium was found to be a prerequisite for the formation of the vivianite layer.
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Imlay, James A. "How obligatory is anaerobiosis?" Molecular Microbiology 68, no. 4 (May 2008): 801–4. http://dx.doi.org/10.1111/j.1365-2958.2008.06213.x.

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Perata, Pierdomenico, and Amedeo Alpi. "Plant responses to anaerobiosis." Plant Science 93, no. 1-2 (January 1993): 1–17. http://dx.doi.org/10.1016/0168-9452(93)90029-y.

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Igamberdiev, Abir U., and Robert D. Hill. "Plant mitochondrial function during anaerobiosis." Annals of Botany 103, no. 2 (June 27, 2008): 259–68. http://dx.doi.org/10.1093/aob/mcn100.

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PÖRTNER, H. O., N. A. ANDERSEN, and N. HEISLER. "Proton-Equivalent Ion Transfer in Sipunculus Nudus as a Function of Ambient Oxygen Tension: Relationships with Energy Metabolism." Journal of Experimental Biology 156, no. 1 (March 1, 1991): 21–39. http://dx.doi.org/10.1242/jeb.156.1.21.

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Proton-equivalent ion transfer processes between animals and ambient water were determined under normoxic control conditions during anaerobiosis and the subsequent recovery period in the marine worm Sipunculus nudus L. During anaerobiosis and recovery, transepithelial H+-equivalent ion transfer was generally correlated with changes in extracellular pH, with some disparities in ‘spring’ animals. The typical initial alkalosis induced by phosphagen cleavage during early anaerobiosis was reflected by a loss of basic equivalents. The acidosis, which developed later, reflecting production of acidic metabolic intermediates, resulted in a relatively small net extrusion of protons into the water. The coelomic acidosis during recovery was greatly exaggerated by the release of protons during phosphagen repletion and by the considerable elevation of Pco2 after normoxia had been reattained. The acidosis stimulated the net release of H+ to the water at a rate several times higher than that during anaerobiosis. The efficient transfer of protons from the body fluids to the environmental water during recovery facilitated normalization of coelomic pH, long before protons dissociated from the large amounts of organic acids produced as anaerobic intermediates could be removed from the body fluids by metabolism. Although the transfer of net H+ equivalents to the water coincided with coelomic acidosis, the rates of transfer during different periods of the experiment were primarily correlated with overall metabolic rate. Low net proton transfer rates associated with anaerobiosis were not sufficient to maintain acid-base parameters typical for normoxia, whereas re-establishment of aerobic conditions facilitated a greatly increased transepithelial H+ transfer rate. These data suggest that the transfer capacity of the energy-consuming translocation mechanism may primarily be determined by the rate of metabolic turnover and, accordingly, by theamount of available energy.
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SALVAT-BRUNAUD, DELPHINE, ANNE THIERRY, and JEAN-LOUIS MAUBOIS. "Development of a medium simulating Emmental juice for propionibacteria growth." Journal of Dairy Research 64, no. 4 (November 1997): 573–80. http://dx.doi.org/10.1017/s0022029997002410.

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Our aim was to develop an Emmental juice-like medium (EJLM) for the growth of propionibacteria. Cheese juices were extracted by pressing three different Emmental cheeses before entering the warm room (i.e. before propionic fermentation) and their composition was determined. They contained (g/kg) lactate 34·6–36·9, protein 23·8–29·6, NaCl 15–23, Ca 5·9–7·3 and no residual sugar, and had a pH of 5·4. In order to simulate this composition, EJLM was constituted from the microfiltrate of a milk, enriched in native casein and fermented by a Lactobacillus helveticus strain until lactose exhaustion. PO43−, K+, Mg2+, Ca2+ and Cl− were added to reach a mineral balance and an ionic strength close to those of juice. The main difference was the nitrogen content: that of juice was ∼3·5 times that of EJLM. Four Propionibacterium freudenreichii strains grew with similar growth rates in EJLM and in juice at 24°C in anaerobiosis and reached >3×109 cfu/ml. Using EJLM, Prop. freudenreichii TL 173 grew at pH 5·4 or 6·0, at 24 or 30°C, in semi-anaerobiosis (static culture) or anaerobiosis (CO2 atmosphere), showing a synergic inhibition of growth at low pH and temperature and in semi-anaerobiosis. EJLM provided a way to study propionibacteria in an environment similar to that in Emmental cheese.
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Turner, Neil A., Giancarlo A. Biagini, and David Lloyd. "Anaerobiosis-induced differentiation of Acanthamoeba castellanii." FEMS Microbiology Letters 157, no. 1 (January 17, 2006): 149–53. http://dx.doi.org/10.1111/j.1574-6968.1997.tb12766.x.

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Dissertations / Theses on the topic "Anaerobiosis"

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Gajardo, Alarcón Ninoska Loreto. "Estudio de la producción de biogás en función de la temperatura en un biodigestor tipo chino." Tesis, Universidad de Chile, 2013. http://www.repositorio.uchile.cl/handle/2250/114944.

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Memoria para optar al título profesional de Ingeniera en Recursos Naturales Renovables
La temperatura es un parámetro importante para el desarrollo de la digestión anaeróbica, que es la base de la producción de biogás; puede limitar sectores para aplicar la tecnología, regular la eficiencia, ayudar en la eliminación de patógenos, afectar el tiempo de retención de la materia e incrementar la producción de biogás. Este trabajo se desarrolló para evaluar la dinámica de producción de biogás en función del régimen térmico, estableciendo la relación entre las temperaturas que afectan a un biodigestor tipo chino y proponer zonas en Chile que posean mayor aptitud para aplicar esta tecnología. El biodigestor utilizado con capacidad de 10 m3, se alimentó tres veces por semana con una mezcla de relación 3:1 agua/estiércol (v/v). Se realizaron monitoreos en dos periodos, febrero a julio (2011) y enero a agosto (2012); en este último se instalaron sensores de registro continuo (temperatura y humedad) y se realizaron análisis de la composición del biogás generado cada semana. En el primer periodo se aplicaron correlaciones lineales a las variables, obteniendo que la temperatura del aire afectó en mayor proporción a la temperatura de descarga, estanque y la del biodigestor. Para el segundo periodo se analizaron las tendencias de las variables, se aplicaron análisis de anomalías para series de tiempo, promedios móviles y ajustes funcionales de las variables más relevantes en el estudio. De los resultados se obtuvo, que la temperatura interna del biodigestor mantuvo una temperatura promedio de 22°C y la temperatura media del suelo se ajustó mejor a la temperatura media máxima del aire. Los análisis de composición fluctuaron de 55 a 85% de CH4 (metano) y de 16 a 35% de CO2 (dióxido de carbono). La zonificación se realizó aplicando el “índice térmico” propuesto en un estudio previo, pero esta vez con información térmica actualizada para todo el país; obteniendo que a partir de la región de Los Lagos y a lo largo de la cordillera de Los Andes, no es apta la aplicación de esta tecnología. Así es posible afirmar que estos biodigestores favorecen la estabilidad térmica interna y que Chile posee un importante potencial para su implementación.
Temperature is an important parameter for the development of anaerobic digestion, base of biogas production. Limit sectors to apply technology, regulate efficiency, assist in the elimination of pathogens, affect the retention time and increase the biogas production. This work was developed to assess, the dynamics of biogas production based on the thermal regime, linking between temperatures affecting a chinese digester and propose areas in Chile that have greater ability to apply this technology. A digester with 10 m3 of capacity was fed three times a week with a 3:1 mixture of water/manure. Monitorings were applied in two periods, february to july (2011) and january to august (2012); installing continuously recording sensors and analyzes compositional of biogas generated weekly. In the first period were applied linear correlations to the variables, it was found that the air temperature affects mainly the discharge, the pond and the digester temperature. In the second period was performed trend analysis, anomalies, moving averages and functional adjustements, it was found that temperature inside of digester was 22°C average and the soil average temperature is more similar with the air average temperature. In the analysis of composition, methane ranged from 55 to 85% and 16 to 35% of carbon dioxide. The zoning was performed by “thermal index”, it was found that since the region of Los Lagos and Andes mountain, range is not suitable the application of this technology. So it can be said that these digesters favor internal thermal stability and that Chile has significant potential for implementation.
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Kelson, Noel. "The effect of dc cardioversion on anaerobic threshold." Thesis, Federation University Australia, 2001. http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/165020.

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Brandão, Andrea Dias. "Papel da redutase do nitrato em soja [Glycine max(L.) Merr. Cv IAC-17] sob condições de hipoxia do sistema radicular." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315242.

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Orientador: Ladaslav Sodek
Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-04T22:11:00Z (GMT). No. of bitstreams: 1 Brandao_AndreaDias_M.pdf: 910878 bytes, checksum: 3dfc539bfdad7f8ba8ddf4be823bb6b0 (MD5) Previous issue date: 2005
Resumo: A soja [Glycine Max (L.) Merr.] tem sido um dos modelos mais usados entre as plantas cultivadas para estudar os efeitos de tolerância à hipóxia, já que é tida como uma planta com certo grau de tolerância ao estresse de oxigênio e sua importância também tem um caráter econômico bastante relevante. Condições de hipóxia são comuns na natureza, por exemplo em campos alagados onde a difusão de oxigênio para o sistema radicular fica bastante prejudicada. Quando o estresse ocorre em tempo prolongado as conseqüências se tornam irreversíveis, levando as plantas à morte, uma vez que são tantos os prejuízos em função da injúria por carência de O2 que acarretam mudanças estruturais em organelas, por exemplo. Nesses casos, mesmo que a disponibilidade de O2 seja restabelecida, a planta não consegue se recuperar. Sabe-se, no entanto, que a presença do nitrato no meio contribui de forma a diminuir e/ou retardar as conseqüências desse processo, aumentando assim a tolerância das plantas a esta condição. Não é conhecido, entretanto, como o nitrato exerce seu efeito benéfico na tolerância à hipóxia. Alguns trabalhos vêm mostrando a ativação da enzima RN (redutase do nitrato) sob este tipo de estresse, um fenômeno que torna interessante a sua participação na tolerância induzida pelo nitrato. Por essa razão esse estudo teve os objetivos de: 1) otimizar método para extração da enzima RN em plantas de soja; 2) avaliar o papel da enzima RN em condições de hipóxia do sistema radicular e 3) Avaliar a utilização e metabolismo do nitrato em condições de hipóxia do sistema radicular. As plantas [Glycine max (L.) Merr. cv IAC-17] foram cultivadas em sistema hidropônico e após atingirem o estádio de desenvolvimento adequado (V4) foram submetidas à hipóxia (pela interrupção do fornecimento de ar comprimido e adição de uma camada de óleo mineral na solução nutritiva do vaso). O período de acompanhamento dos experimentos foi de 4 dias, tempo necessário para que o sistema radicular atingisse a hipóxia. Após esse período também foi estudada a recuperação das plantas do estresse, após a remoção da camada de óleo e restabelecimento da aeração. Foi observado que, independente do pH inicial, o pH da solução nutritiva das plantas em normóxia ficou estável em torno de 7,7 e o das plantas hipóxicas próximos a 6,6. O consumo de nitrato na solução nutritiva das plantas normóxicas e das hipóxicas foi praticamente o mesmo, também foi detectada a presença de nitrito na solução nutritiva dos tratamentos sob hipóxia. Durante o período de recuperação à normóxia, o nitrito acumulado na solução foi gradativamente utilizado. Quanto às raízes, observou-se que em normóxia ocorreu acúmulo de nitrato, enquanto que em hipóxia ocorreu a utilização do nitrato endógeno. Também foi observado nessas condições (hipóxia) o aumento na concentração de aminoácidos livres totais e proteínas em relação às plantas em normóxia. Na seiva do xilema de plantas sob hipóxia foi observada diminuição no teor de nitrato transportado, variação na composição dos aminoácidos livres presentes (aumentando significativamente a proporção de Ala e Gaba e diminuindo principalmente Asn) e aumento do teor de aminoácidos livres totais, em relação às plantas em normóxia. Nas plantas recuperadas, apenas o teor de proteínas da raiz e a composição de aminoácidos livres da seiva do xilema retornaram aos valores encontrados nas plantas normóxicas. Não foi detectada a presença de nitrito no sistema radicular e/ou seiva do xilema das plantas. A atividade da enzima RN foi bastante modificada durante o período de hipóxia diminuindo a valores bem baixos, assim como não houve sua ativação durante o estresse. Em apenas 1 dia de recuperação (retorno à normóxia), sua atividade atingiu os valores das plantas normóxicas. Chegou-se à conclusão de que embora o grau de utilização do nitrato pela planta seja semelhante em normóxia e hipóxia, o seu metabolismo é bastante alterado na hipóxia. Ao contrário do que foi relatado na literatura, a atividade da RN caiu bruscamente durante a hipóxia sem que houvesse evidência de ativação. No entanto, esta alteração na atividade da enzima pode explicar as diferenças no metabolismo do nitrato entre normóxia e hipóxia
Abstract: Soybean [Glycine Max (L.) Merr.] has been frequently used as a model system among cultivated plants in order to study the effects of tolerance to hypoxia, in view of its certain tolerance to oxygen deficiency and its relevant economic importance. Hypoxic conditions are common in nature, for example in flooded fields where the diffusion of oxygen to the root system suffers serious restrictions. When the stress occurs over a long period the consequences can become irreversible leading to the death of the plant in view of the many prejudicial factors arising from oxygen deficiency. These can result in structural changes in organelles, for example, from which the plant is unable to recover on return to normal oxygen availability. It is known, however, that the presence of nitrate in the surrounding medium can diminish or retard the consequences of oxygen deficiency, thereby increasing the tolerance of plants this stress. It is not known, however, what underlies the beneficial effect of nitrate in plant tolerance of hypoxia. Some investigations have shown that nitrate reductase is activated under oxygen stress, a phenomenon that might be related to the tolerance effect of nitrate. Therefore, this study had the objective of: 1) to optmize method for extraction of the RN enzyme in soybean plants; 2)evaluating the role of nitrate reductase during hypoxic stress of the root system and 3) evaluating the utilization and metabolism of nitrate under these stress conditions. Soybean plants [Glycine max (L.) Merr. cv IAC-17] were cultivated in a hydroponic system and after reaching the V4 stage of development were subjected to hypoxia by suspending aeration and applying a layer of mineral oil to the surface of the nutrient solution. The treatment was carried out for 4 days, time enough for the system to undergo hypoxia. Recovery from stress was also studied following hypoxia where the layer of mineral oil was removed from the surface and aeration of the solution restarted. The results showed that independent of the initial pH, the pH of the nutrient solution of normoxic plants stabilized at around 7.7 while that of the plants under hypoxia stabilized at 6.6. The consumption of nitrate in the nutrient solution of the normoxic and hypoxic treatments was practically the same, and nitrite was also detected in the nutrient solution of the plants under hypoxia. During recovery to normoxia, the accumulated nitrite was gradually used. As for the roots, it was observed that under normoxia, nitrate accumulated, while under hypoxia endogenous nitrate was consumed. Besides, under hypoxia, there was an increase in total free amino acids and proteins compared to the controls. In the xylem sap a decline in nitrate was found under hypoxia together with changes in the amino acid composition (where alanine and Gaba increased markedly while Asn diminished) and increases in the total amino acid levels. On return to normóxia only the levels of protein in the root and amino acid composition of the xylem sap returned to values found in the normoxic plants. No nitrite could be detected in the root tissue or xylem sap. Large changes in nitrate reductase activity were found during hypoxia, being reduced to low values. Nor was there any evidence for the activation of the enzyme during the stress. In less than one day on returning to normoxia activity returned tonormoxic plants levels. It was concluded that despite the similarity in the amounts of nitrate utilized comparing plants under normoxia and hypoxia, it was evident that its metabolism was quite different under these two conditions. Contrary to that reported in the literature, nitrate reductase activity falls drastically during hypoxia without any evidence for activation of the enzyme. Nevertheless, the alteration in activity could explain the differences in nitrate metabolism observed between for normóxia and hypoxia
Mestrado
Mestre em Biologia Vegetal
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Carystinos, George D. "Induction of vacuolar H+-translocating pyrophosphatase during anoxia." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55483.

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Anaerobiosis results in low ATP levels and cytoplasmic acidosis. Pyrophosphate (PPi) may play an important role in anaerobiosis as an energy source replacing ATP, as suggested by the hypoxic induction of PPi:fructose 6-phosphate 1-phosphotransferase and sucrose synthase in preference to phosphofructokinase and invertase. Here we show that vacuolar H$ sp+$-translocating pyrophosphatase (PPase) is also strongly induced by anoxia in rice seedlings. The PPase transcript abundance is increased within the first hours of anoxia, and decreases within 2 days after the return of seedlings to air, similarly to alcohol dehydrogenase-1 (Adh1). However, tissue studies show that the highest transcript induction for PPase is in the root whereas the highest induction of Adh1 is in the shoot. Assays of enzyme specific activity indicate a 75-fold increase in PPase activity over 6 days of anoxia, while the vacuolar ATPase changes only slightly. Return of seedlings to air results in rapid disappearance of enzyme activity. Chilling stress in rice seedlings also gives rise to an increase in immunoreactive PPase enzyme, and a progressive 20-fold increase in enzyme specific activity within 6 days. Upon return to room temperature both enzyme level and specific activity decrease. In corn, hypoxic stress results in a small induction in the PPase transcript, and no increase in PPase specific activity, which, however, is constitutively high in this material. It is suggested that in both species, H$ sp+$-PPase may play an important role in hypoxia and chilling stress, not only in conserving ATP, but also in limiting cytoplasmic acidosis.
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Lawrence, Stephen James. "Brewing yeast cell wall gene expression and function during CO2 induced anaerobiosis." Thesis, Oxford Brookes University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.444331.

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McCracken, Andrew. "Metabolic Support of Anaerobiosis in Embryos of the Annual Killifish Austrofundulus limnaeus." PDXScholar, 2012. https://pdxscholar.library.pdx.edu/open_access_etds/600.

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Embryos of the annual killifish Austrofundulus limnaeus display a remarkable tolerance to anoxia during development, most notably during embryonic diapause. Little is known about the metabolic or enzymatic changes that accompany this state of anoxia tolerance. This study examined the metabolic changes associated with exposure to anoxia by measuring the activity of the enzyme phosphoenolpyruvate carboxykinase (PEPCK), and by profiling the concentration of 31 metabolites ranging from amino acids to citric cycle intermediates at 4 different developmental stages, diapause 2 (DII), 4 days post diapause (dpd), 12 and 22 dpd. Embryos of A. limnaeus showed stage specific changes in concentrations of several metabolites. The most notable changes in metabolite concentration in response to anoxia were the increases of lactate, alanine, GABA and succinate as well as a pronounced decrease in aspartate concentrations. However, a complete understanding of the mechanisms by which anoxia tolerance is achieved remains elusive. Further studies into the tissue specific responses of anoxia would enable greater resolution when attempting to explain changes in concentrations of metabolites both during development and in response to anoxic insult.
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Loat, Christopher Eino Russell. "Comparison of the lactate and ventilatory thresholds during prolonged work." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/30153.

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The purpose of this investigation was to compare the ventilatory threshold (T(vent)) with the lactate threshold (T(lact)) during 60 minutes of steady-state exercise at the calculated thresholds. Eight trained, male cyclists (mean age=23.3 yrs, ht=176.4 cm, wt=70.7 kg, VO₂max=61.02 ml/kg‧minˉ¹) performed a 23 W/min progressive intensity cycling test for determination of T(lact) and T(vent). T(vent) was determined by the non-linear increase in excess CO₂ (ExCO₂) while T(lact) was calculated by the 'individual anaerobic threshold' (IAT) method. Subsequently, subjects performed up to 60 minutes steady-state exercise at the threshold workloads. Results at T(vent) and T(lact) indicate significant differences (p<0.01; T(lact)>T(vent)) between VO₂, ExCO₂, HR, [BLa] and workload as calculated by Hotelling's T²-test. During the steady state exercise at each specified workload, VO₂, [BLa], heart rate and ExCO₂ were measured at 15 minute intervals. All subjects completed the steady-state exercise at T(vent) (VSS) while only 2 subjects completed the steady-state exercise at T(lact) (LSS) (avg time=48.4 min). Comparison of metabolic variables using MANOVA and multiple comparisons revealed significant differences between VSS and LSS for HR and VO₂ at all time intervals, for [BLa] at 30 and 45 minute intervals and for ExCO₂ at the 30 minute interval. Furthermore, examination of [BLa] over time using trend analysis revealed a stabilization during VSS ([formula omitted]=3.05 mmol‧Lˉ¹) whereas [BLa] continuously increased over time during LSS. Findings indicate that T(lact) (IAT method) overestimates the ability to perform prolonged work over 45 min. while T(vent) (ExCO) allows for steady-state exercise greater than 60 minutes.
Education, Faculty of
Curriculum and Pedagogy (EDCP), Department of
Graduate
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Bulow, Joseph A. "Reliability of ventilatory threshold using the computerized V- slope method." Virtual Press, 1994. http://liblink.bsu.edu/uhtbin/catkey/902486.

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The ventilatory threshold (VT) is measured frequently during graded exercise tests (GXT) for exercise prescription purposes. The computerized V-slope method for VT determination has been reported to be valid yet little data exists concerning the reliability of the measure. The purpose of this study was to determine the reliability of the VT using the computerized V-slope method. Sixteen healthy volunteers, (eight men and eight women) ages 21-27 (23.6 ± 1.7yrs), performed three maximal GXT on an electronically-braked cycle using an individualized ramp protocol. A minimum of 48 hours separated each test; all three tests were completed within two weeks. Breath-by-breath analysis of gas exchange was performed using a SensorMedics 2900 cart. The VT, expressed as L • min1, was selected by a computerized V-slope method. There were no differences in V02 max between the 3 trials, (overall mean 3.18 ± 0.87 L - min-1). No significant differences were observed for V02 at VT (L - min-1) for tests 1, 2, and 3 (1.62 ± 0.44, 1.58 ± 0.49, and 1.49 ± 0.48) respectively. The VT was determined to be 49.7 ± 7.9% of V02 max. Correlations between the VT in trials 1-2, 1-3, and 2-3 were 0.829, 0.791, and 0.925, respectively. The overall coefficient of variation (C.V.) of the VT measures was 10.61 ± 5.1%. The overall (male and female) variability in VT was 1.28 ± 0.63 METS. Due to high variability and a wide range in correlations, the results failed to support the reliability in the computerized V-slope method.
School of Physical Education
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Robertson, Colin Daniel. "Anerobic catabolism of glycerol by Klebsiellae." Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.330032.

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Wang, Chao. "Gene regulation and metabolic flux reorganization in aerobic/Anaerobic switch of E. coli." HKBU Institutional Repository, 2007. http://repository.hkbu.edu.hk/etd_ra/783.

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Books on the topic "Anaerobiosis"

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International Society for Plant Anaerobiosis., ed. Plant response and adaptation to anaerobiosis. London, UK: Academic Press, 1997.

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Rogers, J. E. Anaerobic transformation processes: A review of the microbiological literature. Athens, GA: U.S. Environmental Protection Agency, Environmental Research Laboratory, 1986.

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Rogers, J. E. Anaerobic transformation processes: A review of the microbiological literature. Athens, GA: U.S. Environmental Protection Agency, Environmental Research Laboratory, 1986.

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Donovan, Eugene. Pilot anaerobic biological treatment of pulp mill evaporator foul condensate. Cincinnati, OH: U.S. Environmental Protection Agency, Water Engineering Research Laboratory, 1986.

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Rappoldt, C. Diffusion in aggregated soil. Wageningen]: Landbouwuniversiteit te Wageningen, 1992.

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Tyson, Kerry Louise. The organisation of Escherichia coli promoters that are regulated by anaerobiosis, nitrite and nitrate. Birmingham: University of Birmingham, 1994.

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B, Jackson Michael, Davies D. D, and Lambers H, eds. Plant life under oxygen deprivation: Ecology, physiology and biochemistry. The Hague, The Netherlands: SPB Academic Pub., 1991.

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Decentralized approach to treating the organic fraction of municipal solid waste with energy recovery by using inclined dry anaerobic digestion. [New York]: Knovel, 2011.

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Oded, Bar-Or, and Skinner James S. 1936-, eds. The Wingate Anaerobic Test. Champaign, IL: Human Kinetics, 1996.

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K, Ahring Birgitte, ed. Biomethanation. Berlin: Springer, 2003.

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Book chapters on the topic "Anaerobiosis"

1

Nakano, Michiko M., and Peter Zuber. "Anaerobiosis." In Bacillus subtilis and Its Closest Relatives, 393–404. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817992.ch28.

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Fausey, N. R., and R. Lal. "Soil Wetness and Anaerobiosis." In Advances in Soil Science, 173–86. New York, NY: Springer New York, 1990. http://dx.doi.org/10.1007/978-1-4612-3322-0_5.

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Reddy, K. Ramesh, Ronald D. DeLaune, and Patrick W. Inglett. "Adaptation of Plants to Soil Anaerobiosis." In Biogeochemistry of Wetlands, 239–80. 2nd ed. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9780429155833-7.

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Wegener, G., and U. Krause. "Environmental and Exercise Anaerobiosis in Frogs." In Surviving Hypoxia, 217–36. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9780367813048-20.

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Thimm, Franz. "Effect of Local Anaerobiosis on Heart Rate." In Advances in Experimental Medicine and Biology, 459–66. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-8181-5_51.

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Knowles, D. J. C., G. T. Grimes, and L. S. Holmes. "Anaerobiosis and Staphylococcal Resistance to Certain 4-Quinolones." In The 4-Quinolones: Anti Bacterial Agents in Vitro, 249–55. London: Springer London, 1990. http://dx.doi.org/10.1007/978-1-4471-3449-7_17.

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Schmid, Georg H., and Klaus P. Bader. "Influence of Anaerobiosis on Oxygen Gas Exchange in the Cyanobacterium Oscillatoria chaybea." In Current Research in Photosynthesis, 865–68. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-0511-5_200.

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Wiedenroth, Ernst Manfred, and Bettina Erdmann. "Anatomy and gas exchange of the roots of wheat seedlings following root anaerobiosis." In Structural and Functional Aspects of Transport in Roots, 215–18. Dordrecht: Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-0891-8_40.

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Wasserman, Karlman, William W. Stringer, and Richard Casaburi. "Is the Slow Component of Exercise $$ (\dot V{o_2}) $$ a Respiratory Adaptation to Anaerobiosis?" In Advances in Experimental Medicine and Biology, 187–94. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-1933-1_36.

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Björn, L. O., I. Panagopoulos, and G. S. Björn. "Ultraweak Luminescence from Plant Tissue: Spectral Characteristics and Effects of Ultraviolet Radiation, Anaerobiosis and Ageing." In Light in Biology and Medicine, 153–59. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5991-3_15.

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Conference papers on the topic "Anaerobiosis"

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Clark, Shirley, Robert Pitt, and Pauline Brown. "Effect of Anaerobiosis on Filter Media Pollutant Retention." In Engineering Foundation Conference 2001. Reston, VA: American Society of Civil Engineers, 2002. http://dx.doi.org/10.1061/40602(263)33.

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Juliana Lobo Paes, Marcio Arêdes Martins, and Lêda Rita D'Antonino Faroni. "Computational Study of Anaerobiosis Acceleration in Hermetic Storage of Maize using Oxygen Depletion." In 2007 Minneapolis, Minnesota, June 17-20, 2007. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2007. http://dx.doi.org/10.13031/2013.23509.

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Teramoto, Jun, Kayoko Yamada, Naoki Kobayashi, Ayako Kori, Shige H. Yoshimura, Kunio Takeyasu, and Akira Ishihama. "Anaerobiosis-induced novel nucleoid protein of Escherichia coli: Architectural role in genome DNA compaction." In 2009 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2009. http://dx.doi.org/10.1109/mhs.2009.5351819.

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Cordeiro, Andre Luiz, Larissa Almeida, Jackeline Leite, Hayssa Mascarenhas, André Guimarães, Luiz Alberto Forgiarini, and Jefferson Petto. "VENTILATORY MUSCLE STRENGTH SIX MONTHS AFTER CORONARY ARTERY BYPASS GRAFTING IN PATIENTS SUBMITTED TO INSPIRATORY MUSCULAR TRAINING BASED ON THE ANAEROBIOSIS THRESHOLD: CLINICAL TRIAL." In ERS International Congress 2021 abstracts. European Respiratory Society, 2021. http://dx.doi.org/10.1183/13993003.congress-2021.pa2214.

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Cordeiro, Andre Luiz, Hayssa Mascarenhas Barbosa, Lucas Landerson, Carolina Lima, Jaclene Araújo, Adriele Souza, Daniel Lago, André Guimarães, and Jefferson Petto. "Inspiratory muscle training based on the anaerobiosis threshold on the functional capacity of patients submitted to coronary arterial bypass grafting: Randomized and controlled clinical trial." In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.pa4142.

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Macêdo, F. D., B. S. Sousa, T. B. Sousa, K. A. Silva, and V. R. F. S. Marães. "Determinação do Limiar de Anaerobiose de Hipertensos." In Congresso Brasileiro de Eletromiografia e Cinesiologia (COBEC) e o Simpósio de Engenharia Biomédica (SEB) - COBECSEB. Uberlândia, Minas Gerais: Even3, 2018. http://dx.doi.org/10.29327/cobecseb.78930.

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HENRIQUE BORDINHON DE CASTRO, PEDRO, TANIA FORSTER CARNEIRO, and Tânia Forster Carneiro. "MONTAGEM DE REATORES ANAEROBIOS COM BAGASSO DE MALTE VISANDO O APROVEITAMENTO ENERGÉTICO." In XXV Congresso de Iniciação Cientifica da Unicamp. Campinas - SP, Brazil: Galoa, 2017. http://dx.doi.org/10.19146/pibic-2017-78080.

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Rosa, Paula Rubia, and Edson Luiz Silva. "ESTRATÉGIAS OPERACIONAIS VISANDO OTIMIZAR A PRODUÇÃO DE HIDROGÊNIO A PARTIR DA MANIPUEIRA EM REATORES ANAEROBIOS DE LEITO FLUIDIZADO." In Simpósio Nacional de Bioprocessos e Simpósio de Hidrólise Enzimática de Biomassa. Campinas - SP, Brazil: Galoá, 2015. http://dx.doi.org/10.17648/sinaferm-2015-33292.

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Santos, Elice Cristina Santos dos, Laila De Souza Gomes, Rutiléia De Jesus Paiva, Patrícia Oliveira Santos, and Andre Da Luz De Freitas. "SIMULAÇÃO DA REAÇÃO DE METANOGÊNESE EM BIORREATOR DE LEITO FIXO." In I Congresso de Engenharia de Biotecnologia. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1357.

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O processo de biodigestão envolve a fermentação anaeróbica a partir de compostos orgânicos realizada por bactérias presentes naturalmente nesses resíduos orgânicos. A reação bioquímica ocorre dentro de um biodigestor, e em processos consecutivos, realizado por diferentes microrganismos específicos, onde o produto final são gases com altos valores energéticos. Esse processo de degradação é dado em quatro fases principais podem ser distinguidas, podendo haver a inclusão de uma quinta fase, dependendo da composição química do despejo a ser tratado.Ao final desse processo, tem-se uma mistura de gases produzidos pela decomposição da matéria orgânica em anaerobiose, composta principalmente de gás metano (CH4) e gás carbônico (CO2), com pequenas quantidades de gás sulfídrico (H2S). Neste contexto, o presente trabalho tem como objetivo simular o processo de biodigestão anaeróbia, com ênfase na reação de metanogênese, utilizando um reator de leito fixo. Além disso, faz-se a elaboração de um planejamento experimental baseando-se em alguns parâmetros para se ter a condição ideal para a maior obtenção de biometano (CH4). Dessa forma, obteve-se o valor de entrada fixo em 19,5 toneladas/dia de matéria orgânica (M.O), e após a variação das variáveis como: Comprimento do biorreator, diâmetro do biorreator, diâmetro do tubo, diâmetro da partícula, porosidade e pressão, obteve-se, em um total de 3 experimentos, o melhor rendimento de Metano (CH4) no valor de 11,5%, onde observou-se que a quantidade de M.O era inversamente proporcional ao rendimento de metano, e que este era diretamente proporcional à largura do biorreator. Portanto, conclui-se que quanto maior a quantidade de toneladas de matéria orgânica no biorreator, menor será a quantidade de metano produzido, bem como quanto maior o comprimento do biorreator, maior a conversão de Biomassa em Biometano. Através desse fato, relacionando o (fluxo de entrada) x (largura do reator), obteve-se rendimentos de 40%, que correspondem aos dados divulgados pela literatura, sendo necessário o uso de múltiplos reatores para viabilidade industrial.
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Silva, Luana Caroline Domingos Da, and Vivianne Lúcia Bormann De Souza. "EFEITO DA RADIAÇÃO IONIZANTE EM SOLUÇÕES CONTENDO SACCHAROMYCES CEREVISIAE." In II Congresso Brasileiro de Biotecnologia On-line. Revista Multidisciplinar de Educação e Meio Ambiente, 2022. http://dx.doi.org/10.51189/conbiotec/16.

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Introdução: A busca por potencializar atividades biológicas com tecnologias diversas tem sido bastante aplicada, incluindo o uso de radiações, que de acordo com a dose empregada influencia na propriedade antimicrobiana. O microrganismo Saccharomyces cerevisiae é aeróbio facultativo, isto é, tem a habilidade de se ajustar metabolicamente, tanto em condições de aerobiose como de anaerobiose. E, sabe-se que a irradiação é capaz de causar danos ao DNA da célula, e alterações em seus produtos finais, que podem ser positivas no sentido de produzir substâncias biologicamente ativas, ou substâncias prejudiciais à saúde humana, com isso é importante averiguar os produtos finais produzidos após a irradiação da Saccharomyces cerevisiae. Objetivo: Analisar estudos recentes e métodos utilizados relacionados a Saccharomyces cerevisiae e a irradiação. Metodologia: A estratégia deste tratou-se de uma revisão sistemática da literatura desenvolvida com o propósito de contribuir para o conhecimento, desenvolvido em cinco etapas: busca da literatura, extração de dados, avaliação dos estudos encontrados, análise e síntese dos resultados. Para condução do estudo, a pesquisa foi realizada entre os meses de maio a junho de 2021, nas bases de dados: Scielo, Brazilian Journal of Development e a Revista Brasileira de Produtos Agroindustriais. Teve como critério de inclusão os artigos relacionados a radiação ionizante e o microrganismo cerevisiae, referente aos últimos 10 anos de publicação e os critérios de exclusão foram produções científicas em formato de tese, dissertação e estudo do caso. Resultados: Foram analisados 3 trabalhos, e cada um desses mostra que a irradiação no microrganismo com a radiação gama, alteração de temperatura e agitação, além da ação da luz branca e a luz UV com doses médias e altas são eficazes na eliminação de microrganismos. Conclusão: Em alguns experimentos realizados pela nossa equipe já se identifica que a radiação reduzia a quantidade de microrganismos, e esses resultados corroboram com os 3 trabalhos analisados. Assim, pode-se afirmar que a radiação pode eliminar microrganismos e age também de forma a torna-lo estático, entretanto ainda estão sendo realizados novos experimentos para verificar possíveis mudanças causadas no microrganismo.
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Reports on the topic "Anaerobiosis"

1

McCracken, Andrew. Metabolic Support of Anaerobiosis in Embryos of the Annual Killifish Austrofundulus limnaeus. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.600.

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Pesis, Edna, Elizabeth J. Mitcham, Susan E. Ebeler, and Amnon Lers. Application of Pre-storage Short Anaerobiosis to Alleviate Superficial Scald and Bitter Pit in Granny Smith Apples. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7593394.bard.

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There is increased demand for high quality fruit produced and marketed with reduced chemical inputs to minimize toxic effects on human health and the environment. Granny Smith (GS) apple quality is reduced by two major physiological disorders, superficial scald and bitter pit (BP). These disorders cause great loss to apple growers worldwide. Superficial scald is commonly controlled by chemical treatments, mainly the antioxidant diphenylamine (DPA) and/or the ethylene action inhibitor, 1-methylcyclopropene (1–MCP). Both chemicals are ineffective in controlling bitter pit incidence. We proposed to investigate the beneficial use of non-chemical, abiotic stress with low O2 (LO2) applied for 10d at 20°C on GS apple fruit. During the project we expanded the treatment to more apple cultivars, Golden Delicious (GD) and Starking Delicious (SD) and another pome fruit, the pear. Apple and pear have similar physiological disorders that develop during cold storage and we examined if the LO2 treatment would also be effective on pear. Application of 0.5% LO2 atmosphere for 10d at 20°C or 500ppb 1-MCP at 20°C prior to cold storage at 0°C, was effective in reducing superficial scald in GS apple. Moreover, LO2 pretreatment was also effective in reducing bitter pit (BP) development in California GS and Israeli GD and SD apples The BP symptoms in GS from California were much more prominent, so the effect of LO2 was more dramatic than the effect on the Israeli cvs. GD and SD, nevertheless the LO2 treatment showed the same trend in all cultivars in reducing BP. The LO2 and 1-MCP -treated fruit exhibited lower levels of ethylene, - farnesene and its oxidation product, 6-methyl-5-hepten-2-one (MHO), as determined by SPME/GC-MS analysis. In addition, LO2 pretreatment applied to California Bartlett or Israeli Spadona pears was effective in reducing superficial scald, senescent scald and internal breakdown after 4 m of cold storage at 0°C. For GS apple, low-temperature storage resulted in oxidative stress and chilling injury, caused by increased production of superoxide anions which in turn led to the generation of other dangerous reactive oxygen species (ROS). Using confocal laser-scanning microscopy and H2O2 measurements of apple peel, we observed ROS accumulation in control fruit, while negligible amounts were found in LO2 and 1-MCP treated fruit. Gene-expression levels of ROS-scavenging enzymes were induced by the various pretreatments: catalase was induced by LO2 treatment, whereas Mn superoxide dismutase was induced by 1-MCP treatment. We assume that LO2 and 1-MCP pretreated fruit remained healthier due to reduced production of ethylene and reactive oxygen substances, such as MHO, during cold storage. The LO2-treated apple exhibited greener peel and firmer fruit after 6 m of cold storage, and the fruit had high crispiness leading to high taste preference. In both pear cultivars, the LO2 treatment led to a reduction in internal breakdown and browning around the seed cavity. We tested the LO2 pre-storage treatment on a semi-commercial scale that would be applicable to a small organic grower by sealing the fruit within the plastic field bins. The treatment was most effective with a continuous flow of nitrogen through the bins; however, a single 6 hour flush of nitrogen was also fairly effective. In addition, we determined that it was very important to have the oxygen levels below 0.5% for approximately 10 days to achieve good scald control, not counting the time required to reduce the oxygen concentration. Our LO2 technology has been proven in this project to be effective in reducing several physiological disorders developed in pome fruit during cold storage. We hope that our non-chemical treatment which is friendly to the environment will be used in the near future for the organic apple and pear industry. The next step should be an analysis of the cost-benefits and commercial feasibility.
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Pesis, Edna, Chaim Frenkel, and Philip E. Shaw. Mechanisms of Action and Uses of Anaerobiosis and Anaerobic Intermediates for the Post Harvest Regulation of Fruit Quality. United States Department of Agriculture, October 1986. http://dx.doi.org/10.32747/1986.7566710.bard.

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Pesis, Edna, and Mikal Saltveit. Postharvest Delay of Fruit Ripening by Metabolites of Anaerobic Respiration: Acetaldehyde and Ethanol. United States Department of Agriculture, October 1995. http://dx.doi.org/10.32747/1995.7604923.bard.

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The use of pretreatments for 24 h prior to storage, under anaerobic condtions, or in the presence of the natural metabolic products, acetaldehyde (AA) and ethanol, to delay fruit ripening, was found to be effective with several climacteric fruits, among them avocado, mango, peach and tomato. The delay in ripening of avocado, peach and tomato was accompanied by inhibition of ethylene production and of fruit softening. The maintenance of fruit firmness was associated with a decrease in the activities of cell-wall-degrading enzymes, including endoglucanases (Cx), polygalacturonases (PG) and b-galactosidases. In peaches the AA- and N2-treated fruits were firmer after 3 weeks storage and contained higher amount of insoluble pectin than untreated controls. We showed that AA vapors are able to inhibit ripening, ethylene production and ethylene induction in the presence of 1-amino-cyclopropane-1-carboxylic acid (ADD) in avocado and mango tissue. Ethylene induced by ACC is taken as an indicator of ACC oxidase activity. ACC oxidase activity in AA-treated avocado fruit was much lower than in the untreated fruit. In carnation flowers very little ethylene was produced by ethanol-treated flowers, and the normal increases in ACC content and ACC oxidase activity were also suppressed. Using kinetic studies and inhibitors of alcohol dehydrogenase (ADH), we showed that AA, not ethanol, was the active molecule in inhibiting ripening of tomato fruit. Application of anaerobiosis or anaerobic metabolites was effective in reduction of chilling injury (CI) in various plant tissues. Pretreatment with a low-O2 atmosphere reduced CI symptoms in avocado; this effect was associated with higher content of the free sylfhydryl (SH) group, and induction of the detoxification enzymes, catalase and peroxidase. Application of AA maintained firmer and brighter pulp tissue (non-oxidative), which was associated with higher free SH content, lower ethylene and ACC oxidase activities, and higher activities of catalase and peroxidase. Ethanol was found to reduce CI in other plant tissue. In roots of 24-h-old germinated cucumber seeds, exposure to 0.4-M ethanol shock for 4 h reduced chilling-induced ion leakage. In cucumber cotyledons it appears that alcohols may reduce CI by inducing stomata closure. In cotyledon discs held in N2 at 10C for 1 day, there accumulated sufficient endogenously synthesized ethanol to confer tolerance to chilling at 2.5C for 5 days.
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