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Journal articles on the topic 'Anaerobe E.coli K12'

Author: Grafiati

Published: 6 September 2023

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1

Commodari, Fernando, and Brian K. Hunter. "Production and interconversion of 1,2-propanediol and hydroxyacetone by Escherichia coli." Biochemistry and Cell Biology 67, no. 8 (August 1, 1989): 468–72. http://dx.doi.org/10.1139/o89-074.

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The anaerobic metabolism of marginally lethal levels of [13C]formaldehyde by Escherichia coli (K12, MU352, CRB, and CR63) was followed in vivo by 13C NMR. The products include 1, 2-propanediol. Under aeration, the 1, 2-propanediol is converted to hydroxyacetone. The hydroxyacetone is reconverted to 1, 2-propanediol when aeration is stopped. The process can be cycled by varying the rate of aeration.Key words: hydroxyacetone, 1, 2-propanediol, Escherichia coli, production.

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2

SCHAMBERGER, GERRY P., and FRANCISCO DIEZ-GONZALEZ. "Selection of Recently Isolated Colicinogenic Escherichia coli Strains Inhibitory to Escherichia coli O157:H7." Journal of Food Protection 65, no. 9 (September 1, 2002): 1381–87. http://dx.doi.org/10.4315/0362-028x-65.9.1381.

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Escherichia coli strains were screened for their ability to inhibit E. coli O157:H7. An initial evaluation of 18 strains carrying previously characterized colicins determined that only colicin E7 inhibited all of the E. coli O157:H7 strains tested. A total of 540 strains that had recently been isolated from humans and nine different animal species (cats, cattle, chickens, deer, dogs, ducks, horses, pigs, and sheep) were tested by a flip-plating technique. Approximately 38% of these strains were found to inhibit noncolicinogenic E. coli K12 strains. The percentage of potentially colicinogenic E. coli per animal species ranged from 14% for horse isolates to 64% for sheep strains. Those isolates that inhibited E. coli K12 were screened against E. coli O157:H7, and 42 strains were found to be capable of inhibiting all 22 pathogenic strains tested. None of these 42 strains produced bacteriophages, and only 24 isolates inhibited serotype O157:H7 in liquid culture. The inhibitory activity of these strains was completely eliminated by treatment with proteinase K. When mixtures of these 24 colicinogenic strains were grown in anaerobic continuous culture, the four-strain E. coli O157:H7 population was reduced at a rate of 0.25 log10 cells per ml per h, which was fivefold faster than the washout rate. Two strains originally isolated from cat feces (F16) and human feces (H30) were identified by repetitive sequences polymerase chain reaction as the predominant isolates in continuous cultures. The results of this work indicate that animal species other than cattle can be sources of anti-O157 colicinogenic strains, and these results also lead to the identification of at least two isolates that could potentially be used in preharvest control strategies.

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3

Ralph, E. T., J. R. Guest, and J. Green. "Altering the anaerobic transcription factor FNR confers a hemolytic phenotype on Escherichia coli K12." Proceedings of the National Academy of Sciences 95, no. 18 (September 1, 1998): 10449–52. http://dx.doi.org/10.1073/pnas.95.18.10449.

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4

Mat-Jan, Fairoz, Charling R. Williams, and David P. Clark. "Anaerobic growth defects resulting from gene fusions affecting succinyl-CoA synthetase in Escherichia coli K12." Molecular and General Genetics MGG 215, no. 2 (January 1989): 276–80. http://dx.doi.org/10.1007/bf00339728.

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5

Asare, Paul Tetteh, Katrin Zurfluh, Anna Greppi, Denise Lynch, Clarissa Schwab, Roger Stephan, and Christophe Lacroix. "Reuterin Demonstrates Potent Antimicrobial Activity Against a Broad Panel of Human and Poultry Meat Campylobacter spp. Isolates." Microorganisms 8, no. 1 (January 6, 2020): 78. http://dx.doi.org/10.3390/microorganisms8010078.

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Reuterin is a broad-spectrum antimicrobial system produced by specific strains of Lactobacillus reuteri during anaerobic metabolism of glycerol. Acrolein is the main component responsible for its antimicrobial activity. Here, the sensitivity of Campylobacter jejuni (n = 51) and Campylobacter coli (n = 20) isolates from chicken meat and human stool samples to reuterin was investigated. The minimum inhibitory concentration (MIC) of C. jejuni and C. coli strains was measured between 1.5 and 3.0 µM of acrolein, below the MIC of the sensitive indicator strain Escherichia coli K12 (16.5 µM acrolein). The interaction of C. jejuni N16-1419 and the reuterin-producing L. reuteri PTA5_F13 was studied during 24 h co-cultures with or without glycerol. A high C. jejuni growth was observed in cultures without glycerol. In contrast, C. jejuni growth decreased from 7.3 ± 0.1 log CFU/mL to below detection limit (1 log CFU/mL) during co-cultures added with 28 mM glycerol. This bactericidal effect could be attributed to in situ reuterin production. The low MIC observed and the high sensitivity towards in situ produced reuterin suggests L. reuteri combined with glycerol, as a possible intervention option to reduce Campylobacter in the food chain.

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6

Portnoy, Vasiliy A., Markus J. Herrgård, and Bernhard Ø. Palsson. "Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain." Applied and Environmental Microbiology 74, no. 24 (October 24, 2008): 7561–69. http://dx.doi.org/10.1128/aem.00880-08.

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ABSTRACT Fermentation of glucose to d-lactic acid under aerobic growth conditions by an evolved Escherichia coli mutant deficient in three terminal oxidases is reported in this work. Cytochrome oxidases (cydAB, cyoABCD, and cbdAB) were removed from the E. coli K12 MG1655 genome, resulting in the ECOM3 (E. coli cytochrome oxidase mutant) strain. Removal of cytochrome oxidases reduced the oxygen uptake rate of the knockout strain by nearly 85%. Moreover, the knockout strain was initially incapable of growing on M9 minimal medium. After the ECOM3 strain was subjected to adaptive evolution on glucose M9 medium for 60 days, a growth rate equivalent to that of anaerobic wild-type E. coli was achieved. Our findings demonstrate that three independently adaptively evolved ECOM3 populations acquired different phenotypes: one produced lactate as a sole fermentation product, while the other two strains exhibited a mixed-acid fermentation under oxic growth conditions with lactate remaining as the major product. The homofermenting strain showed a d-lactate yield of 0.8 g/g from glucose. Gene expression and in silico model-based analyses were employed to identify perturbed pathways and explain phenotypic behavior. Significant upregulation of ygiN and sodAB explains the remaining oxygen uptake that was observed in evolved ECOM3 strains. E. coli strains produced in this study showed the ability to produce lactate as a fermentation product from glucose and to undergo mixed-acid fermentation during aerobic growth.

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7

Beaumont, M. D., and H. M. Hassan. "Characterization of regulatory mutations causing anaerobic derepression of the sodA gene in Escherichia coli K12: cooperation between cis- and trans-acting regulatory loci." Journal of General Microbiology 139, no. 11 (November 1, 1993): 2677–84. http://dx.doi.org/10.1099/00221287-139-11-2677.

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8

Lu, Jingrang, Tammie L. Gerke, Helen Y. Buse, and Nicholas J. Ashbolt. "Development of an Escherichia coli K12-specific quantitative polymerase chain reaction assay and DNA isolation suited to biofilms associated with iron drinking water pipe corrosion products." Journal of Water and Health 12, no. 4 (June 6, 2014): 763–71. http://dx.doi.org/10.2166/wh.2014.203.

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A quantitative polymerase chain reaction assay (115 bp amplicon) specific to Escherichia coli K12 with an ABITM internal control was developed based on sequence data encoding the rfb gene cluster. Assay specificity was evaluated using three E. coli K12 strains (ATCC W3110, MG1655 & DH1), 24 non-K12 E. coli and 23 bacterial genera. The biofilm detection limit was 103 colony-forming units (CFU) E. coli K12 mL−1, but required a modified protocol, which included a bio-blocker Pseudomonas aeruginosa with ethylenediaminetetraacetic acid buffered to pH 5 prior to cell lysis/DNA extraction. The novel protocol yielded the same sensitivity for drinking water biofilms associated with Fe3O4 (magnetite)-coated SiO2 (quartz) grains and biofilm-surface iron corrosion products from a drinking water distribution system. The novel DNA extraction protocol and specific E. coli K12 assay are sensitive and robust enough for detection and quantification within iron drinking water pipe biofilms, and are particularly well suited for studying enteric bacterial interactions within biofilms.

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9

Ung, Porsry, Chanthol Peng, Sokunsreiroat Yuk, Vannak Ann, Hasika Mith, Reasmey Tan, Kazuhiko Miyanaga, and Yasunori Tanji. "Fate of Escherichia coli in dialysis device exposed into sewage influent and activated sludge." Journal of Water and Health 16, no. 3 (April 18, 2018): 380–90. http://dx.doi.org/10.2166/wh.2018.282.

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Abstract Tracing the fate of pathogens in environmental water, particularly in wastewater, with a suitable methodology is a demanding task. We investigated the fate of Escherichia coli K12 in sewage influent and activated sludge using a novel approach that involves the application of a biologically stable dialysis device. The ion concentrations inside the device could reach that of surrounding solution when it was incubated in phosphate buffered saline for 2 h. E. coli K12 above 107 CFU mL−1 (inoculated in distilled water, influent, activated sludge) were introduced into the device and incubated in influent and activated sludge for 10 days. Without indigenous microorganisms, E. coli K12 could survive even with the limited ions and nutrients concentrations in influent and activated sludge. E. coli K12 abundance in influent and activated sludge were reduced by 60 and 85%, respectively, after just 1 day. The establishment of microbial community in wastewater played an important role in reducing E. coli K12. Bacteriophage propagated in filtered influent or activated sludge when E. coli K12 was introduced, but not in raw influent or activated sludge. The methodology developed in this study can be applied in the actual environmental water to trace the fate of pathogens.

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10

Dreyfus, Marc, and Valérie Heurgué-Hamard. "Termination troubles in Escherichia coli K12." Molecular Microbiology 79, no. 2 (November 29, 2010): 288–91. http://dx.doi.org/10.1111/j.1365-2958.2010.07468.x.

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11

Corcuera, G. L., M. Bastidas, and M. Dubourdieu. "Molybdenum uptake in Escherichia coli K12." Journal of General Microbiology 139, no. 8 (August 1, 1993): 1869–75. http://dx.doi.org/10.1099/00221287-139-8-1869.

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12

Jebril, Nadia Mahmoud Tawfiq. "In Vitro Bioremediation: A Development Process of Cadmium and Mercury Removal by Environmental Biotechnologies of UV-Mutated Escherichia coli K12 and Bacillus subtilis 168." Baghdad Science Journal 17, no. 1(Suppl.) (March 18, 2020): 0244. http://dx.doi.org/10.21123/bsj.2020.17.1(suppl.).0244.

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coli K12 and B. subtilis 168 were investigated for their cadmium and mercury tolerance abilities. They were developed by UV mutagenesis technique to increase their tolerances either to cadmium or mercury, and their names then were designated depend on the name and concentration of metals. E. coli K12 Cd3R exhibited bioremediation amount of 6.5 mg Cd/g dry biomass cell. At the same time, its wild-type (E. coli K12 Cd3) was able to remove 5.2 mg Cd/g dry biomass cell in treatment of 17 mg Cd /L within 72 hours of incubation at 37 °C (pH=7) in vitro assays. The results show that E.coli K12 Hg 20 was able to remove 0.050 µg Hg/g dry biomass cell and more removal by its mutant E.coli K12 Hg 20R to 0.060 µg Hg/ g dry biomass cell in the treatment of 0.15 µg Hg /L. On the other hand, B. subtilis168 Cd2 was able to remove the least amount of cadmium (5 mg Cd/ g dry biomass cell) and of mercury (0.045 µg Hg/ g dry biomass cell) under the same conditions were used for E. coli K12. Also, the complete removal of both metals was confirmed by scanning electron microscopy (SEM) showing that the effect of cadmium and mercury on the bacterial mass. Also, the SEM images showed that the removal amounts had relationships in changing the morphology of cells under in vitroassays.

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13

Alokam, Suneetha, Shu-Lin Liu, Kamal Said, and Kenneth E. Sanderson. "Inversions over the Terminus Region in Salmonella and Escherichia coli: IS200s as the Sites of Homologous Recombination Inverting the Chromosome of Salmonella enterica Serovar Typhi." Journal of Bacteriology 184, no. 22 (November 15, 2002): 6190–97. http://dx.doi.org/10.1128/jb.184.22.6190-6197.2002.

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ABSTRACT Genomic rearrangements (duplications and inversions) in enteric bacteria such as Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K12 are frequent (10−3 to 10−5) in culture, but in wild-type strains these genomic rearrangements seldom survive. However, inversions commonly survive in the terminus of replication (TER) region, where bidirectional DNA replication terminates; nucleotide sequences from S. enterica serovar Typhimurium LT2, S. enterica serovar Typhi CT18, E. coli K12, and E. coli O157:H7 revealed genomic inversions spanning the TER region. Assuming that S. enterica serovar Typhimurium LT2 represents the ancestral genome structure, we found an inversion of 556 kb in serovar Typhi CT18 between two of the 25 IS200 elements and an inversion of about 700 kb in E. coli K12 and E. coli O157:H7. In addition, there is another inversion of 500 kb in E. coli O157:H7 compared with E. coli K12. PCR analysis confirmed that all S. enterica serovar Typhi strains tested, but not strains of other Salmonella serovars, have an inversion at the exact site of the IS200 insertions. We conclude that inversions of the TER region survive because they do not significantly change replication balance or because they are part of the compensating mechanisms to regain chromosome balance after it is disrupted by insertions, deletions, or other inversions.

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14

Liu, D., and P. R. Reeves. "Escherichia coli K12 regains its O antigen." Microbiology 140, no. 1 (January 1, 1994): 49–57. http://dx.doi.org/10.1099/13500872-140-1-49.

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15

Sørensen, Søren Johannes. "Survival of Escherichia coli K12 in seawater." FEMS Microbiology Ecology 8, no. 2 (April 1991): 161–67. http://dx.doi.org/10.1111/j.1574-6941.1991.tb01720.x.

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16

Parrott, S., S. Jones, and R. A. Cooper. "2-Phenylethylamine Catabolism by Escherichia coli K12." Microbiology 133, no. 2 (February 1, 1987): 347–51. http://dx.doi.org/10.1099/00221287-133-2-347.

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17

Weber, Richard F., and Philip M. Silverman. "The Cpx proteins of Escherichia coli K12." Journal of Molecular Biology 203, no. 2 (September 1988): 467–78. http://dx.doi.org/10.1016/0022-2836(88)90013-7.

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18

Sørensen, S. "Survival of Escherichia coli K12 in seawater." FEMS Microbiology Letters 85, no. 2 (April 1991): 161–67. http://dx.doi.org/10.1016/0378-1097(91)90512-9.

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19

Membrillo-Hernández, Jorge, Alejandra Núñez-De La Mora, Tania Del Rio-Albrechtsen, Rafael Camacho-Carranza, and M. Carmen Gomez-Eichelmann. "Thermally-induced cell lysis inEscherichia coli K12." Journal of Basic Microbiology 35, no. 1 (1995): 41–46. http://dx.doi.org/10.1002/jobm.3620350112.

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20

Eltysheva, I. V., L. E. Zatvornitskiy, and I. L. Maslennikova. "INFLUENCE OF ANTIBACTERIAL FACTORS ON THE MIXED CULTURE OF ESCHERISCHIA COLI AND STAPHYLOCOCCUS EPIDERMIDIS UNDER CONDITIONS OF INCREASED OSMOTIC PRESSURE." Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology, no. 1 (2020): 19–25. http://dx.doi.org/10.17072/1994-9952-2020-1-19-25.

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Commensal strains of the human intestine Escherichia coli K12 and of the human skin Staphylococcus epi-dermidis are often found in the same atypical biotopes, where they can act as infectious agents. The metabo-lism of bacteria changes in polymicrobial associations and in the presence of increased osmotic pressure in biotopes, associated with a number of pathological conditions that may affect their sensitivity to antibacterial factors of various nature. In this regard, the aim of the work was to study the effectiveness of chlorampheni-col, ceftriaxone, polyvalent pyobacteriophage and chlorhexidine on single and mixed cultures of opportunistic microorganisms E. coli K12 and S. epidermidis at a background of 2% NaCl. It was shown that the effect of ceftriaxone, a broad-spectrum antibiotic, and chloramphenicol, with antibacterial properties only against E. coli, were leveled under conditions of increased osmotic pressure at concentrations of 12-200 mg/l in a mixed culture. For E. coli K12, a decrease in the effectiveness of the bacteriophage at high osmotic pressure was noted, which is the opposite in effect towards S. epidermidis. As for chlorhexidine, its effect on E. coli K12 and S. epidermidis was enhanced in single and mixed cultures under conditions of 2% NaCl. In general, in most cases, an increase in the osmotic pressure of the medium acted as a synergist for inhibiting bacterial growth with antibiotics and bacteriostatic.

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21

FRATAMICO, PINA M., FRANKIE J. SCHULTZ, ROBERT C. BENEDICT, ROBERT L. BUCHANAN, and PETER H. COOKE. "Factors Influencing Attachment of Escherichia coli O157:H7 to Beef Tissues and Removal Using Selected Sanitizing Rinses‡." Journal of Food Protection 59, no. 5 (May 1, 1996): 453–59. http://dx.doi.org/10.4315/0362-028x-59.5.453.

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Attachment of E. coli O157:H7 and E. coli K12 to beef tenderloin filet, chuck, and adipose tissues was studied. Most attachment occurred within 1 min of incubation; the number of attached organisms depended on the concentration of bacteria in the liquid inoculum. Similar levels of E. coli bound to the three types of beef tissues tested. E. coli O157:H7 was heavily piliated; however, there was no significant difference between levels of bound E. coli O157:H7 and E. coli K12, indicating that these surface structures apparently are not involved in attachment. Scanning electron photomicrographs of meat tissue and of purified collagen suggested that bacteria attached primarily to collagen fibers. Rinsing solutions consisting of 10% trisodium phosphate (TSP), 2% acetic acid (HAc), phosphate-buffered saline (PBS) and combinations of each were tested for effectiveness in reducing the number of attached E. coli. The level of bacteria removed from tenderloin tissue following TSP, HAc, or PBS rinses did not differ considerably. When beef tissues were stored at 4°C for 18 h after the various rinse combinations, TSP rinse treatments reduced the levels of E. coli K12 and O157:H7 attached to adipose tissue up to 3.4 and 2.7 log units, respectively, compared to PBS rinse treatments. Therefore, TSP may be effective for reducing populations of E. coli O157:H7 on beef carcass tissue.

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22

Jeter, Cecelia, and Ann G. Matthysse. "Characterization of the Binding of Diarrheagenic Strains of E. coli to Plant Surfaces and the Role of Curli in the Interaction of the Bacteria with Alfalfa Sprouts." Molecular Plant-Microbe Interactions® 18, no. 11 (November 2005): 1235–42. http://dx.doi.org/10.1094/mpmi-18-1235.

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Diarrheagenic Escherichia coli were able to bind to plant surfaces, including alfalfa sprouts and open seed coats, and tomato and Arabidopsis thaliana seedlings incubated in water. The characteristics of the binding differed with the bacterial strain examined. Laboratory K12 strains of E. coli failed to show significant binding to any of the plant surfaces examined, suggesting that some of the genes present and expressed in pathogenic strains and absent or unexpressed in K12 strains may be required for binding to plants. When a plasmid carrying the mlrA gene (a positive regulator of curli biosynthesis) or a plasmid carrying the operons that encode the synthesis of curli (csgA-G) was introduced into K12 strains, the bacteria acquired the ability to bind to sprouts. CsgA mutants of an avian pathogenic E. coli and an O157:H7 strain showed no reduction in their ability to bind to sprouts. Thus, the production of curli appears to be sufficient to allow K12 strains to bind, but curli are not necessary for the binding of pathogenic strains, suggesting that pathogenic strains may have more than one mechanism for binding to plant surfaces.

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23

Edgar, Robert H., Anie-Pier Samson, Tori Kocsis, and John A. Viator. "Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria." Micromachines 14, no. 3 (February 28, 2023): 573. http://dx.doi.org/10.3390/mi14030573.

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Photoacoustic flow cytometry is a method to detect rare analytes in fluids. We developed photoacoustic flow cytometry to detect pathological cells in body fluids, such as circulating tumor cells or bacteria in blood. In order to induce specific optical absorption in bacteria, we use modified bacteriophage that precisely target bacterial species or subspecies for rapid identification. In order to reduce detection variability and to halt the lytic lifescycle that results in lysis of the bacteria, we attached dyed latex microspheres to the tail fibers of bacteriophage that retained the bacterial recognition binding sites. We tested these microsphere complexes using Salmonella enterica (Salmonella) and Escherichia coli (E. coli) bacteria and found robust and specific detection of targeted bacteria. In our work we used LT2, a strain of Salmonella, against K12, a strain of E. coli. Using Det7, a bacteriophage that binds to LT2 and not to K12, we detected an average of 109.3±9.0 of LT2 versus 2.0±1.7 of K12 using red microspheres and 86.7±13.2 of LT2 versus 0.3±0.6 of K12 using blue microspheres. These results confirmed our ability to selectively detect bacterial species using photoacoustic flow cytometry.

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24

Otaki, Masahiro, Kazuyoshi Yano, and Shinichiro Ohgaki. "Virus removal in a membrane separation process." Water Science and Technology 37, no. 10 (May 1, 1998): 107–16. http://dx.doi.org/10.2166/wst.1998.0387.

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Recently, membrane technology has been considered an alternative to conventional water purification. To study the fate of viruses in membrane processes, indigenous coliphages in pilot scale membrane processes located in the eastern part of Tokyo Metropolitan area have been surveyed for 6 months. This plant used river water as its resource and had two microfiltration membrane processes which had different pore sizes (0.2 μm and 0.1 μm) and one ultrafiltration process which had 13,000 nominal molecular weight cut off. To detect indigenous coliphages, E. coli K12 F+(A/λ) and E. coli C were used as host bacteria. E. coli K12 F+(A/λ) can detect both DNA and RNA phages and E. coli C can only DNA phage. The resource water contained E. coli K12 phages at 200∼1500 PFU/100 mL and the removal ratio of these DNA and RNA phages was lower than that of DNA phage by E. coli C in both MF membrane processes through 6 months. It is thought to be caused by difference of phage size, because DNA phage is bigger than RNA phage in general. The removal ratio of E. coli K12 and E. coli C phages reached 100% in the UF membrane process. According to the comparison of the concentration of phages in solution and eluted from suspended solid in resource and drain, it is thought that most phages concentrated in the drain were absorbed in suspended solids. To make certain of the removal ratio in UF and NF (nanofiltration) processes, high concentrations of coliphage Qβ and poliomyelitis virus vaccine were fed into these processes. The removal ratio of coliphage Qβ in UF and NF processes are 10−8.3 and 10−6.3 respectively, and the ratio of poliomyelitis virus vaccine in UF and NF are <10−6.7 and <10−7.3 respectively.

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25

Saif, Nehal A., Yomna A. Hashem, Heba M. Amin, and Ramy K. Aziz. "In Silico and In Vitro Investigation of the Distribution and Expression of Key Genes in the Fucose Operon of Escherichia coli." Microorganisms 11, no. 5 (May 11, 2023): 1265. http://dx.doi.org/10.3390/microorganisms11051265.

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Many gut bacteria degrade polysaccharides, providing nutritional advantages to their hosts. Fucose, a mucin degradation product, was suggested as a communication molecule between the resident microbiota and external pathogens. However, the precise role and variants of the fucose utilization pathway remain to be elucidated. Here, we computationally and experimentally investigated the fucose utilization operon of E. coli. While the operon is conserved among E. coli genomes, a variant pathway, in which an ABC transporter system replaces the fucose permease gene (fucP), was computationally identified in 50 out of 1058 genomes. Comparative genomics and subsystems analysis results were confirmed by polymerase chain reaction-based screening of 40 human E. coli isolates, which indicated the conservation of fucP in 92.5% of the isolates (vs. 7.5% of its suggested alternative, yjfF). The in silico predictions were confirmed by in vitro experiments comparing the growth of E. coli strains K12, BL21, and isogenic fucose-utilization K12 mutants. Additionally, fucP and fucI transcripts were quantified in E. coli K12 and BL21, after in silico analysis of their expression in 483 public transcriptomes. In conclusion, E. coli utilizes fucose by two pathway variants, with measurable transcriptional differences. Future studies will explore this variation’s impact on signaling and virulence.

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26

Pugsley, A. P., F. Moreno, and V. De Lorenzo. "Microcin-E492-insensitive Mutants of Escherichia coli K12." Microbiology 132, no. 12 (December 1, 1986): 3253–59. http://dx.doi.org/10.1099/00221287-132-12-3253.

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27

Debbia, E. A. "Filamentation promotes F'lac loss in Escherichia coli K12." Journal of General Microbiology 138, no. 10 (October 1, 1992): 2083–91. http://dx.doi.org/10.1099/00221287-138-10-2083.

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28

Rosales-Colunga, Luis, and Agustino Martínez-Antonio. "Engineering Escherichia coli K12 MG1655 to use starch." Microbial Cell Factories 13, no. 1 (2014): 74. http://dx.doi.org/10.1186/1475-2859-13-74.

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29

Burstein, Claude, and Adam Kepes. "The melibiose permease system of Escherichia coli K12." Biochimie 67, no. 1 (January 1985): 59–67. http://dx.doi.org/10.1016/s0300-9084(85)80230-3.

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30

Satishchandran, C., and G. D. Markham. "Adenosine-5′-phosphosulfate Kinase from Escherichia coli K12." Journal of Biological Chemistry 264, no. 25 (September 1989): 15012–21. http://dx.doi.org/10.1016/s0021-9258(18)63804-9.

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31

Wright, B. E., A. Longacre, and J. M. Reimers. "Hypermutation in derepressed operons of Escherichia coli K12." Proceedings of the National Academy of Sciences 96, no. 9 (April 27, 1999): 5089–94. http://dx.doi.org/10.1073/pnas.96.9.5089.

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32

PEBORDE, J., and D. SAMAIN. "Preparation of triated lipopolysaccharides from Esherichia coli K12." Biochimica et Biophysica Acta (BBA) - General Subjects 1033, no. 2 (February 26, 1990): 207–9. http://dx.doi.org/10.1016/0304-4165(90)90014-n.

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33

Maksimova, Yu G., and Ya E. Bykova. "Effect of multi-walled carbon nanotubes on Escherichia coli biofilm formation." Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology, no. 2 (2021): 87–92. http://dx.doi.org/10.17072/1994-9952-2021-2-87-92.

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The effect of purified and unpurified multi-walled carbon nanotubes on the biofilm formation of Esche-richia coli strains isolated from different sources has been studied. It has been shown that carbon nano-materials in the culture medium do not inhibit biofilm formation, but on days 1–3 of growth lead to the formation of more massive biofilms of some strains. Significantly more intense destruction of mature bio-films of E. coli K12, E. coli K12 TG1 (pXen7) and one natural strain in the presence of carbon nanotubes in the medium was noted. No clear dependence of biofilm formation and destruction of formed biofilms on the degree of purification of nanotubes was found.

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34

Жиленков, Александр Викторович, Екатерина Александровна Хакина, Павел Анатольевич Трошин, Ильшат Файзелгаянович Каримов, and Дмитрий Геннадьевич Дерябин. "Водорастворимые анионоидные производные C₆₀-фуллерена проявляют свойство антидотов в отношении ионов Hg (II) при тестировании на клетках Escherichia coli." Химико-фармацевтический журнал 53, no. 4 (June 7, 2019): 24–29. http://dx.doi.org/10.30906/0023-1134-2019-53-4-24-29.

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В высокоспецифичных реакциях хлорфуллерена C60Cl6 получены 3 водорастворимых производных C60-фуллерена, фунционализированных анионоидными аддендами различного химического состава и структуры. Установлено, что в водном окружении в присутствии стехиометрического (по молям 1:1) количества синтезированных производных C60-фуллерена и ионов Hg (II) происходит формирование гибридных органо-металлических комплексов, в ряде случаев сопровождающееся их агрегацией. Результатом подобного взаимодействия является снижение биодоступности ионов Hg (II) для клеток Escherichia coli, проявляющееся через уменьшение токсичности в отношении штамма E. coli K12 TG1 pF1 или ослабление защитных реакций штамма E. coli K12 MG1655 pMerA’::lux. Наиболее выраженный защитный эффект зарегистрирован с использованием производного C60-фуллерена, функционализированного серосодержащими аддендами общей формулы -S(CH2)10CO2K, по уровню антитоксической активности сопоставимого с фармакопейным препаратом «Унитиолом».

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35

Alzahrani, Fatimah M., Stephen G. Yeates, Michelle Webb, and Hind Ali Alghamdi. "Antibacterial Activity of Tannic Acid and Tannic Acid/Amphiphilic Cationic Polymer Mixtures." Asian Journal of Chemistry 32, no. 6 (2020): 1491–96. http://dx.doi.org/10.14233/ajchem.2020.21776.

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In this study, the antibacterial activity of tannic acid/amphiphilic cationic polymer (poly{2-[(methacryloyloxy)ethyl]trimethyl-ammonium chloride}, PMADQUAT) and tannic acid mixtures was examined on the strains of Gram-positive (S. aureus) and Gram-negative (E. coli CI2, E. coli K12, Klebsiella pneumonia and P. aeruginosa) bacteria. Tannic acid exhibited the antibacterial activity against all the studied bacterial strains. The ester linkage between glucose and gallic acid is vital for the antimicrobial activity of tannic acid. Tannic acid inhibited the growth of S. aureus and E. coli K12 (1 wt%) and reduced the growth of P. aeruginosa to 23%. Mixing cationic polymers having different structures (statistical copolymer, homopolymer and diblock polymer) with tannic acid lead to an increase in antibacterial activity of tannic acid and the stability and clarity of mixtures was higher than that of a pure tannic acid solution. Tannic acid/diblock polymer and tannic acid/homopolymer mixtures (0.1 wt%) were excellent for inhibiting the growth of planktonic E. coli K12 bacteria, and a low concentration (0.0001 wt%) of tannic acid/diblock polymer reduced its growth to 19%. By contrast, the tannic acid/statistical polymer mixture (0.0001 wt%) was excellent for inhibiting the growth of Gram-positive S. aureus bacteria.

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36

Elena V. Sorokina, Ilya R. Vodolazov, and Alexander V. Oleskin. "Stimulatory and Toxic Effects of Neurotransmitters on the lux Operon-Dependent Bioluminescence of Escherichia coli K12 TGI." Journal of Pharmacy and Nutrition Sciences 9, no. 3 (June 5, 2019): 136–43. http://dx.doi.org/10.29169/1927-5951.2019.09.03.1.

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Background: The normal functioning of the brain requires neuromediators, i.e., substances that transmit messages between nervous cells. Neurochemicals also function as signals that are involved in communication among the microorganisms that inhabit the human organism. While the impact of “classical” neurotransmitters including catecholamines, serotonin, and histamine on microorganisms has been investigated in a number of recent publications, this work provides evidence for the stimulatory and inhibitory (toxic) effects of some other important neurochemicals that have not received sufficient attention in the literature.Methods: The biosensor was based on a GM Escherichia coli K12 strain (TGI) that contained the lux operon of the luminescent soil bacterium Photorhabdus luminescencens ZMI. The biosensor was exposed to the action of the tested neurotransmitters for 15 mins to 144 hrs. The intensity of bacterial luminescence (counts / second) was monitored in the control and the experimental samples with an 1251 BioOrbit luminometer (Finland).Results: Neurochemicals such as putrescine, acetylcholine, taurin, and indole were found to stimulate, at low concentrations (0.1-10 µM), the luminescence of the strain E. coli K12 TGI containing the lux operon from Photorhabdus luminescencens ZMI. At higher concentrations, putrescine, taurin, and indole exerted a weak toxic influence, i.e. they marginally attenuated the luminescence of E. coli K12 TGI.Conclusions: Based on the data obtained, a regulatory, presumably receptor-dependent, effect is exerted by the tested neurochemicals on the bacterium E. coli K12 TGI, in an analogy to their impact on nervous, immune, and other specialized types of eukaryotic cells. However, high neurochemical concentrations are likely to produce nonspecific effects on the bacterial luciferase system and/or on membrane phosphorylation.

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Benson, S. A., M. N. Hall, and T. J. Silhavy. "Genetic Analysis of Protein Export in Escherichia Coli K12." Annual Review of Biochemistry 54, no. 1 (June 1985): 101–34. http://dx.doi.org/10.1146/annurev.bi.54.070185.000533.

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38

Gottesman, S., and V. Stout. "Regulation of capsular polysaccharide synthesis in Escherichia coli K12." Molecular Microbiology 5, no. 7 (July 1991): 1599–606. http://dx.doi.org/10.1111/j.1365-2958.1991.tb01906.x.

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39

Nakamatsu, Eduardo H., Erica Fujihira, Rita C. C. Ferreira, AndrÃ©a Balan, SÃ©rgio O. P. Costa, and LuÃs C. S. Ferreira. "Oligopeptide uptake and aminoglycoside resistance in Escherichia coli K12." FEMS Microbiology Letters 269, no. 2 (April 2007): 229–33. http://dx.doi.org/10.1111/j.1574-6968.2007.00634.x.

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40

Smith, C., J. Econome, A. Schutt, S. Klco, and C. Cantor. "A physical map of the Escherichia coli K12 genome." Science 236, no. 4807 (June 12, 1987): 1448–53. http://dx.doi.org/10.1126/science.3296194.

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TRUN, N. J., and T. J. SILHAVY. "The genetics of protein targeting in Escherichia coli K12." Journal of Cell Science 1989, Supplement 11 (January 1, 1989): 13–28. http://dx.doi.org/10.1242/jcs.1989.supplement_11.2.

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42

Cornet, Iris, Eva Van Derlinden, Astrid M. Cappuyns, Wim Bruyninckx, Agnes Kovacs, and Jan F. Van Impe. "The heterogeneous heat stress response of Escherichia coli K12." Procedia Food Science 1 (2011): 1060–66. http://dx.doi.org/10.1016/j.profoo.2011.09.158.

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43

Sung, Y. C., and J. A. Fuchs. "Characterization of the cyn operon in Escherichia coli K12." Journal of Biological Chemistry 263, no. 29 (October 1988): 14769–75. http://dx.doi.org/10.1016/s0021-9258(18)68104-9.

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Ganesan, Ann K., Joyce Hunt, and Philip C. Hanawalt. "Temperature dependent survival of UV-irradiated Escherichia coli K12." Molecular and General Genetics MGG 214, no. 2 (October 1988): 198–203. http://dx.doi.org/10.1007/bf00337711.

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Roberts, Anne, Seok-Yong Lee, Emma McCullagh, Ruth E. Silversmith, and David E. Wemmer. "Ybiv from Escherichia coli K12 is a HAD phosphatase." Proteins: Structure, Function, and Bioinformatics 58, no. 4 (January 18, 2005): 790–801. http://dx.doi.org/10.1002/prot.20267.

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46

Clark, Alvin J. "recA mutants ofE. coli K12: A personal turning point." BioEssays 18, no. 9 (September 1996): 767–72. http://dx.doi.org/10.1002/bies.950180912.

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47

Atlung, Tove, Erik S. Clausen, and Flemming G. Hansen. "Autoregulation of the dnaA gene of Escherichia coli K12." Molecular and General Genetics MGG 200, no. 3 (August 1985): 442–50. http://dx.doi.org/10.1007/bf00425729.

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48

Cherchi, C., and A. Z. Gu. "Effect of bacterial growth stage on resistance to chlorine disinfection." Water Science and Technology 64, no. 1 (July 1, 2011): 7–13. http://dx.doi.org/10.2166/wst.2011.536.

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The mechanisms and factors that affect microbial resistance to chlorine disinfection have not been fully elucidated. In this study, we investigated the impact of the cell growth stage on chlorine disinfection efficiency. Specifically, we evaluated the impact of the growth stage on chlorination resistance by comparing the inactivation efficiencies of two indicator bacterial strains (Escherichia coli K12 and Escherichia coli 0157:H7) obtained from various growth phases, using Chick-Watson kinetic parameters. For both E. coli strains (K12 and 0157:H7), the inactivation rate constants are the lowest at stationary phase (0.19 and 0.32) compared to those at initial lag (0.54 and 0.76) and exponential growth phase (0.63 and 0.69), respectively. These results suggested that the abundance of resistant subpopulations increases at stressed stationary conditions and E. coli cells obtained from the stationary growth phase exhibited more resistance and lower inactivation efficiency compared to those from the lag and exponential phases. This implies that microbes in wastewater treatment process with varying solids retention times (SRTs, which indicate growth rates) may show different extents of chlorine resistance. Comparison of the coefficient of dilution (n) values in both E. coli strains for the various growth phases suggest that cells seem to be more sensitive to disinfectant concentration at the stationary-lag phase than that at the exponential stage. Comparing the two E. coli strains, higher inactivation rates were observed for the pathogenic O157:H7 than for K12 at different stages of growth. The strain-to-strain variability in survivability to chlorine exposure has to be considered when selecting indicator microorganisms for water quality monitoring.

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Almarashi, Jamal F. M., Natalia Kapel, Thomas S. Wilkinson, and Helmut H. Telle. "Raman Spectroscopy of Bacterial Species and Strains Cultivated under Reproducible Conditions." Spectroscopy: An International Journal 27 (2012): 361–65. http://dx.doi.org/10.1155/2012/540490.

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Rapid and reproducible discrimination between bacterial pathogens is a clear goal in microbiological laboratories when processing infected clinical samples. In this study Raman spectra were taken from at least 30 colonies of four strains of bacteria includingStaphylococcus epidermidis(1457 and 9142) andEscherichia coli(K12 and Top 10) using the Renishawin ViaRaman microscope system. Analysis based on principal components suggests that even strain differentiation (e.g., 1457 versus 9142 or K12 versus Top10) is possible.

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Kricker, Maja, and Barry G. Hall. "Biochemical Genetics of the Cryptic Gene System for Cellobiose Utilization in Escherichia coli K12." Genetics 115, no. 3 (March 1, 1987): 419–29. http://dx.doi.org/10.1093/genetics/115.3.419.

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ABSTRACT The cellobiose catabolic system of Escherichia coli K12 is being used to study the role of cryptic genes in microbial evolution. Wild-type E. coli K12 do not utilize the β-glucoside sugars, arbutin, salicin and cellobiose. A Cel+ (cellobiose utilizing) mutant which grows on cellobiose, arbutin, and salicin was isolated previously from wild-type E. coli K12. Biochemical assays indicate that a cel structural gene (celT) specifies a single transport protein that is a β-glucoside specific enzyme of the phosphoenolpyruvate-dependent phosphotransferase system. The transport protein phosphorylates β-glucosides at the expense of phosphoenolpyruvate. A single phosphoglucosidase, specified by celH, hydrolyzes phosphorylated cellobiose, arbutin, and salicin. The genes of the cel system are expressed constitutively in the Cel+ mutant, whereas they are not expressed at a detectable level in the wild-type strain. The transport and hydrolase genes are simultaneously silenced or simultaneously expressed and thus constitute an operon. Cel+ strains which fail to utilize one or more β-glucosides express the transport system at a lower level than do Cel+ strains which grow on all three β-glucosides. Other strains inducibly express a gene which specifies transport of arbutin but not the other β-glucosides. The arbutin transport gene, arbT, maps outside of the cel locus.

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