Journal articles: 'Amyloliquefaciens'

1

Zheng, Yangyang, Xudong Wang, Siyuan Liu, Kewei Zhang, Zhibo Cai, Xiuling Chen, Yao Zhang, Jiayin Liu, and Aoxue Wang. "The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato." International Journal of Molecular Sciences 19, no. 8 (July 30, 2018): 2221. http://dx.doi.org/10.3390/ijms19082221.

Full text

Abstract:

To investigate whether the ech42 gene in Clonostachysrosea can improve the biocontrol efficacy of Bacillus amyloliquefaciens and its molecular mechanism. Compared to the wild type, the B. amyloliquefaciens transformed with the ech42 gene exhibited higher chitinase activity. The B. amyloliquefaciens-ech42 also showed significantly higher biocontrol efficiency compared to Botrytiscinerea when tomato plants were pre-treated with B. amyloliquefaciens-ech42. No significant difference in biocontrol efficiency was observed between the wild type and B.amyloliquefaciens-ech42 when tomato plants were first infected by Botrytiscinerea. In addition, the activity of the defense-related enzyme polyphenol oxidase, but not superoxide dismutase, was significantly higher in B. amyloliquefaciens-ech42 than in the wild type. The ech42 enhances the biocontrol efficiency of B.amyloliquefaciens by increasing the capacity of preventative/curative effects in plants, rather than by killing the pathogens.

APA, Harvard, Vancouver, ISO, and other styles

2

Ahsan, Taswar, Chaoqun Zang, Shuyi Yu, Xue Pei, Jinhui Xie, Ying Lin, Xiaozhou Liu, and Chunhao Liang. "Screening, and Optimization of Fermentation Medium to Produce Secondary Metabolites from Bacillus amyloliquefaciens, for the Biocontrol of Early Leaf Spot Disease, and Growth Promoting Effects on Peanut (Arachis hypogaea L.)." Journal of Fungi 8, no. 11 (November 20, 2022): 1223. http://dx.doi.org/10.3390/jof8111223.

Full text

Abstract:

A novel Bacillus amyloliquefaciens BAM strain, with novel fermentation nutrient mediums and compositions, could produce potent antifungal secondary metabolites, as the existing strains face resistance from fungus pathogens. In the current study, we introduced two novel nutrient mediums for the fermentation process, semolina and peanut root extract, as carbon and nitrogen sources in order to maximize the antifungal effects of B. amyloliquefaciens against Cercaspora arachidichola to control early leaf spot disease in peanuts. Based on a single-factor test and the central composite design of response surface methodology, the optimum fermentation medium for Bacillus amyloliquefaciens antagonistic substance was determined, containing 15 gm/L of semolina flour, 12.5 gm/L of beef extract, and 0.5 gm/L of magnesium sulfate, which inhibited the fungal growth by 91%. In vitro, antagonistic activity showed that the fermentation broth of B. amyloliquefaciens BAM with the optimized medium formulation had an inhibition rate of (92.62 ± 2.07)% on the growth of C. arachidichola. Disease control effects in pot experiments show that the pre-infection spray of B. amyloliquefaciens BAM broth had significant efficiency of (92.00 ± 3.79)% in comparison to post-infection spray. B. amyloliquefaciens BAM broth significantly promoted peanut plant growth and physiological parameters and reduced the biotic stress of C. archidechola. Studies revealed that B. amyloliquefaciens BAM with a novel fermentation formulation could be an ideal biocontrol and biofertilizer agent and help in early disease management of early leaf spots in peanuts.

APA, Harvard, Vancouver, ISO, and other styles

3

Ranjith, Sellappan, Thangavel Kalaiselvi, Muruganagounder Muthusami, and Uthandi Sivakumar. "Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (Spodoptera frugiperda) in Maize." Microorganisms 10, no. 9 (September 16, 2022): 1850. http://dx.doi.org/10.3390/microorganisms10091850.

Full text

Abstract:

Maize is an important cereal crop which is severely affected by Spodoptera frugiperda. The study aims to identify endophytic bacteria of maize root and leaf apoplastic fluid with bioprotective traits against S. frugiperda and plant growth promoting properties. Among 15 bacterial endophytic isolates, two strains—namely, RAF5 and LAF5—were selected and identified as Alcaligenes sp. MZ895490 and Bacillus amyloliquefaciens MZ895491, respectively. The bioprotective potential of B. amyloliquefaciens was evaluated through bioassays. In a no-choice bioassay, second instar larvae of S. frugiperda fed on B. amyloliquefaciens treated leaves (B+) recorded comparatively lesser growth (1.10 ± 0.19 mg mg−1 day−1) and consumptive (7.16 ± 3.48 mg mg−1 day−1) rates. In larval dip and choice bioassay, the same trend was observed. In detached leaf experiment, leaf feeding deterrence of S. frugiperda was found to be greater due to inoculation with B. amyloliquefaciens than Alcaligenes sp. The phenolics content of B. amyloliquefaciens inoculated plant was also found to be greater (3.06 ± 0.09 mg gallic acid g−1). However, plant biomass production was more in Alcaligenes sp inoculated treatment. The study thus demonstrates the potential utility of Alcaligenes sp. and B. amyloliquefaciens for improving growth and biotic (S. frugiperda) stress tolerance in maize.

APA, Harvard, Vancouver, ISO, and other styles

4

Fang, Haitian, Huiyan Liu, Ning Chen, Chenglin Zhang, Xixian Xie, and Qingyang Xu. "Site-directed mutagenesis studies on the uridine monophosphate binding sites of feedback inhibition in carbamoyl phosphate synthetase and effects on cytidine production by Bacillus amyloliquefaciens." Canadian Journal of Microbiology 59, no. 6 (June 2013): 374–79. http://dx.doi.org/10.1139/cjm-2012-0758.

Full text

Abstract:

A major problem when pyrimidine de novo biosynthesis is used for cytidine production is the existence of many negative regulatory factors. Cytidine biosynthesis in Bacillus amyloliquefaciens proceeds via a pathway that is controlled by uridine monophosphate (UMP) through feedback inhibition of carbamoyl phosphate synthetase (CPS), the enzyme that converts CO2, NH3, and glutamine to carbamoyl phosphate. In this study, the gene carB encoding the large subunit of CPS from B. amyloliquefaciens CYT1 was site directed, and the UMP binding sites of feedback inhibition in Bam-CPS are described. The residues Thr-941, Thr-970, and Lys-986 in CPS from B. amyloliquefaciens were subjected to site-directed mutagenesis to alter UMP’s feedback inhibition of CPS. To find feedback-resistant B. amyloliquefaciens, the influence of the T941F, T970A, K986I, T941F/K986I, and T941F/T970A/K986I mutations on CPS enzymatic properties was studied. The recombinant B. amyloliquefaciens with mutated T941F/K986I and T941F/T970A/K986I CPS showed a 3.7- and 5.7-fold increase, respectively, in cytidine production in comparison with the control expressing wild-type CPS, which was more suitable for further application of the cytidine synthesis. To a certain extent, the 5 mutations were found to release the enzyme from UMP inhibition and to improve B. amyloliquefaciens cytidine-producing strains.

APA, Harvard, Vancouver, ISO, and other styles

5

Dunlap, Christopher A., Soo-Jin Kim, Soon-Wo Kwon, and Alejandro P. Rooney. "Phylogenomic analysis shows that Bacillus amyloliquefaciens subsp. plantarum is a later heterotypic synonym of Bacillus methylotrophicus." International Journal of Systematic and Evolutionary Microbiology 65, Pt_7 (July 1, 2015): 2104–9. http://dx.doi.org/10.1099/ijs.0.000226.

Full text

Abstract:

The rhizosphere-isolated bacteria belonging to the Bacillus amyloliquefaciens subsp. plantarum and Bacillus methylotrophicus clades are an important group of strains that are used as plant growth promoters and antagonists of plant pathogens. These properties have made these strains the focus of commercial interest. Here, we present the draft genome sequence of B. methylotrophicus KACC 13105T ( = CBMB205T). Comparative genomic analysis showed only minor differences between this strain and the genome of the B. amyloliquefaciens subsp. plantarum type strain, with the genomes sharing approximately 95 % of the same genes. The results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the type strains of these two taxa are highly similar. In fact, our results show that the type strain of B. amyloliquefaciens subsp. plantarum FZB42T ( = DSM 23117T = BGSC 10A6T) does not cluster with other members of the B. amyloliquefaciens taxon. Instead, it clusters well within a clade of strains that are assigned to B. methylotrophicus, including the type strain of that species. Therefore, we propose that the subspecies B. amyloliquefaciens subsp. plantarum should be reclassified as a later heterotypic synonym of B. methylotrophicus.

APA, Harvard, Vancouver, ISO, and other styles

6

Nan, Jing, Shaoran Zhang, and Ling Jiang. "Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing." Plants 10, no. 2 (January 29, 2021): 261. http://dx.doi.org/10.3390/plants10020261.

Full text

Abstract:

Citrus huanglongbing (HLB) is a destructive disease caused by Candidatus Liberibacter species and is a serious global concern for the citrus industry. To date, there is no established strategy for control of this disease. Previously, Bacillus amyloliquefaciens GJ1 was screened as the biocontrol agent against HLB. In this study, two-year-old citrus infected by Ca. L. asiaticus were treated with B. amyloliquefaciens GJ1 solution via root irrigation. In these plants, after seven irrigation treatments, the results indicated that the photosynthetic parameters, chlorophyll content, resistance-associated enzyme content and the expression of defense-related genes were significantly higher than for the plants treated with the same volume water. The content of starch and soluble sugar were significantly lower, compared to the control treatment. The parallel reaction monitoring (PRM) results revealed that treatment with B. amyloliquefaciens GJ1 solution, the expression levels of 3 proteins with photosynthetic function were upregulated in citrus leaves. The accumulation of reactive oxygen species (ROS) in citrus leaves treated with B. amyloliquefaciens GJ1 flag22 was significantly higher than untreated plants and induced the defense-related gene expression in citrus. Finally, surfactin was identified from the fermentation broth of B. amyloliquefaciens GJ1 by high-performance liquid chromatography. These results indicate that B. amyloliquefaciens GJ1 may improve the immunity of citrus by increasing the photosynthesis and enhancing the expression of the resistance-related genes.

APA, Harvard, Vancouver, ISO, and other styles

7

Wang, Da-Cheng, Chun-Hao Jiang, Li-Na Zhang, Lin Chen, Xiao-Yun Zhang, and Jian-Hua Guo. "Biofilms Positively Contribute to Bacillus amyloliquefaciens 54-induced Drought Tolerance in Tomato Plants." International Journal of Molecular Sciences 20, no. 24 (December 12, 2019): 6271. http://dx.doi.org/10.3390/ijms20246271.

Full text

Abstract:

Drought stress is a major obstacle to agriculture. Although many studies have reported on plant drought tolerance achieved via genetic modification, application of plant growth-promoting rhizobacteria (PGPR) to achieve tolerance has rarely been studied. In this study, the ability of three isolates, including Bacillus amyloliquefaciens 54, from 30 potential PGPR to induce drought tolerance in tomato plants was examined via greenhouse screening. The results indicated that B. amyloliquefaciens 54 significantly enhanced drought tolerance by increasing survival rate, relative water content and root vigor. Coordinated changes were also observed in cellular defense responses, including decreased concentration of malondialdehyde and elevated concentration of antioxidant enzyme activities. Moreover, expression levels of stress-responsive genes, such as lea, tdi65, and ltpg2, increased in B. amyloliquefaciens 54-treated plants. In addition, B. amyloliquefaciens 54 induced stomatal closure through an abscisic acid-regulated pathway. Furthermore, we constructed biofilm formation mutants and determined the role of biofilm formation in B. amyloliquefaciens 54-induced drought tolerance. The results showed that biofilm-forming ability was positively correlated with plant root colonization. Moreover, plants inoculated with hyper-robust biofilm (ΔabrB and ΔywcC) mutants were better able to resist drought stress, while defective biofilm (ΔepsA-O and ΔtasA) mutants were more vulnerable to drought stress. Taken altogether, these results suggest that biofilm formation is crucial to B. amyloliquefaciens 54 root colonization and drought tolerance in tomato plants.

APA, Harvard, Vancouver, ISO, and other styles

8

Wei, Xubiao, Xiudong Liao, Jun Cai, Zhaojun Zheng, Lulu Zhang, Tingting Shang, Yu Fu, Cong Hu, Lei Ma, and Rijun Zhang. "Effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat." Animal Production Science 57, no. 9 (2017): 1899. http://dx.doi.org/10.1071/an16119.

Full text

Abstract:

An experiment was conducted to investigate the effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat. 440 1-day-old Arbor Acres male broiler chicks were randomly allocated into five dietary treatments with eight replicates in a completely randomised design for 42 days. The treatments were as follows: basal diet (control) or the basal diet supplemented with 107 colony-forming units (CFU) B. amyloliquefaciens/kg (B7), 108 CFU B. amyloliquefaciens/kg (B8), 109 CFU B. amyloliquefaciens/kg (B9) or 40 mg aureomycin/kg (antibiotic). The results showed that B. amyloliquefaciens-supplemented groups had greater (P < 0.05) average daily gain during Days 1 to 21, Days 22 to 42 and Days 1 to 42, and improved (P < 0.05) feed conversion rate during Days 1 to 42 compared with the control. Broilers in the B7 and B9 groups had higher (P < 0.05) average daily feed intake than those in the control group in all experimental phases. Broilers fed either B. amyloliquefaciens or antibiotic diet showed higher (P < 0.05) a* value, b* value and lower L* value in the breast muscle than those in the control group. Dietary B. amyloliquefaciens supplementation increased (P < 0.05) the contents of C14:1, C16:1c, C18:1t, C18:1c, C20:1n-9, total monounsaturated fatty acids, C18:2c, C18:3n-3, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3, C22:6n-3 and total polyunsaturated fatty acids as well as ratio of polyunsaturated fatty acids to saturated fatty acids in breast muscle and the concentrations of C17:1, C20:1n-9, C18:2t-9, t-12, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3 and C22:6n-3 in thigh muscle of broilers. The results indicated that B. amyloliquefaciens improved broilers’ growth performance, meat quality and fatty acid composition.

APA, Harvard, Vancouver, ISO, and other styles

9

Domenico Prisa. "Antifungal and plant growth promoting activity with Bacillus amyloliquefaciens in Aeonium subs." Magna Scientia Advanced Biology and Pharmacy 1, no. 2 (February 28, 2021): 042–50. http://dx.doi.org/10.30574/msabp.2021.1.2.0008.

Full text

Abstract:

In this work, the biostimulant and biocontrol capacity of Bacillus amyloliquefaciens in succulent plants such as Aeonium was evaluated. The specific objectives were in particular: (i) Evaluate whether the use of this bacterium can increase the growth rate of Aeonium plants that typically have a slow growth cycle; (ii) evaluate if the use of B. amyloliquefaciens can lead to increased plant resistance to Fusarium oxysporum; (iii) considered if utilization of B. amyloliquefaciens allows for increased plant resistance to water and nutrient stress. The experimentation showed a significant improvement of the agronomic parameters analyzed on all Aeonium plants treated with Bacillus amyloliquefaciens. In general there was a significant increase in plant height, number of leaves, vegetative and root weight, number of new shoots. In addition, Aeonium goochiae and sunburst show a better protection against the fungus Fusarium oxysporum. The trial also highlighted the ability of B. amyloliquefaciens to guarantee a productive and qualitative production of the plants even under conditions of reduced water and nutritional resources. Plants, thanks to the interaction with soil microorganisms, can reach higher nutrient and water resources, resulting in greater resistance to abiotic stresses and better quality in the cultivation cycle.

APA, Harvard, Vancouver, ISO, and other styles

10

Pasaribu, T., E. B. Laconi, and I. P. Kompiang. "Evaluation of the nutrient contents of palm kernel cake fermented by microbial cocktails as a potential feedstuff for poultry." Journal of the Indonesian Tropical Animal Agriculture 44, no. 3 (September 24, 2019): 295. http://dx.doi.org/10.14710/jitaa.44.3.295-302.

Full text

Abstract:

The aims of this research were to improve the nutritive value of palm kernel cake by fermentation technology using Bacillus amyloliquefacien, Trichoderma harzianum and cocktail microbes (combination of Bacillus amyloliquefacien and Trichoderma harzianum). Data were analyzed using Completely Randomized Design Factorial, with experiments consisting of types of microbes (B. amyloliquefaciens, T. harzianum, microbial cocktail) as treatment and incubation time (0, 3, 5, and 7 days). Parameters were crude protein and crude fiber for all treatments, and the lowest fiber analysis would continue with NDF, ADF, crude fat, and amino acids. Result showed that the three of microbes grew on palm kernel meal in third incubation and grew on and in the substrate at 7 days. microbial cocktails increased protein and reduced crude fiber better than B. amyloliquefacien and T. harzianum on palm kernel cake fermentation technology. Cocktail microbes enhanced amino acids such as methionine, arginine, and glutamic acid, also neutral detergent fiber but reduced ADF and hemicellulose. It was concluded that the palm kernel cake fermented with microbial cocktails can reduced crude fiber and increase crude protein with an incubation period of 7 days.

APA, Harvard, Vancouver, ISO, and other styles

11

Jinno, Cynthia N., and Yanhong Liu. "109 Awardee Talk: Dietary Supplementation of Bacillus Amyloliquefaciens Influenced Systemic Immunity and Intestinal Microbiome of Weaned Pigs." Journal of Animal Science 100, Supplement_2 (April 12, 2022): 51. http://dx.doi.org/10.1093/jas/skac064.080.

Full text

Abstract:

Abstract Supplementation of various Bacillus spp. has shown to improve growth performance, immune responses, and gut health of newly weaned pigs. The present study investigated the effects of dietary supplementation of 109 CFU/kg of Bacillus (B.) amyloliquefaciens on growth performance, diarrhea, systemic immunity, and intestinal microbiome of weaned pigs with or without enterotoxigenic Escherichia coli (ETEC) challenge. An antibiotic treatment was also included in the experiment to compare the efficacy of B. amyloliquefaciens vs. carbadox (50 mg/kg). ETEC challenge reduced (P < 0.05) growth rate but increased (P < 0.05) the frequency of diarrhea in pigs throughout the experiment. Supplementation of B. amyloliquefaciens tended (P < 0.10) to increase growth performance but had limited impacts on diarrhea of weaned pigs. ETEC challenge increased (P < 0.05) white blood cell (WBC) count on d 7 and 21 post-inoculation (PI), while supplementation of B. amyloliquefaciens tended (P < 0.10) to reduce WBC on d 7 PI and reduced (P < 0.05) WBC on d 21 PI, compared with control. No differences were observed in performance, diarrhea, and systemic immunity of pigs when comparing B. amyloliquefaciens with carbadox. Supplementation of B. amyloliquefaciens and antibiotics had different impacts on intestinal microbiome. ETEC challenge increased (P < 0.05) relative abundance of Lactobacillus in feces on d 7 PI but reduced (P < 0.05) relative abundance of Prevotella in feces on d 21 PI. Prior ETEC challenge, Lachnospiraceae was less (P < 0.05) abundant in feces of pigs supplemented with B. amyloliquefaciens than pigs in carbadox group on d 0 before ETEC infection. The relative abundance of Enterobacteriaceae in fecal samples was the lowest (P < 0.05) in pigs supplemented with carbadox among all treatment on d 0. On d 14 and 21 PI, the relative abundance of Actinobacteria in feces was the lowest (P < 0.05) in carbadox treatment among all treatment of the experiment. Ileal digesta were also collected on d 21 PI to analyze intestinal microbiota diversity changes among treatments. The Shannon diversity index was greater (P < 0.05) in ileal digesta of pigs supplemented with B. amyloliquefaciens than pigs supplemented with carbadox. ETEC reduced (P < 0.05) the relative abundance of Firmicutes and Lactobacillus and increased (P < 0.05) the relative abundance of Proteobacteria and Lachnospiraceae in ileal digesta. Under ETEC challenge, pigs supplemented with B. amyloliquefaciens had lower (P < 0.05) abundance of Firmicutes and Clostridiaceae and greater (P < 0.05) abundance of Lachnospiraceae in the ileum than pigs supplemented with carbadox. In conclusion, supplementation of B. amyloliquefaciens had similar impacts on growth performance and systemic immunity but had different impacts on intestinal microbiota of weaned pigs in comparison to carbadox.

APA, Harvard, Vancouver, ISO, and other styles

12

Borriss, Rainer, Xiao-Hua Chen, Christian Rueckert, Jochen Blom, Anke Becker, Birgit Baumgarth, Ben Fan, et al. "Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons." International Journal of Systematic and Evolutionary Microbiology 61, no. 8 (August 1, 2011): 1786–801. http://dx.doi.org/10.1099/ijs.0.023267-0.

Full text

Abstract:

The whole-genome-sequenced rhizobacterium Bacillus amyloliquefaciens FZB42T (Chen et al., 2007) and other plant-associated strains of the genus Bacillus described as belonging to the species Bacillus amyloliquefaciens or Bacillus subtilis are used commercially to promote the growth and improve the health of crop plants. Previous investigations revealed that a group of strains represented a distinct ecotype related to B. amyloliquefaciens; however, the exact taxonomic position of this group remains elusive (Reva et al., 2004). In the present study, we demonstrated the ability of a group of Bacillus strains closely related to strain FZB42T to colonize Arabidopsis roots. On the basis of their phenotypic traits, the strains were similar to Bacillus amyloliquefaciens DSM 7T but differed considerably from this type strain in the DNA sequences of genes encoding 16S rRNA, gyrase subunit A (gyrA) and histidine kinase (cheA). Phylogenetic analysis performed with partial 16S rRNA, gyrA and cheA gene sequences revealed that the plant-associated strains of the genus Bacillus, including strain FZB42T, formed a lineage, which could be distinguished from the cluster of strains closely related to B. amyloliquefaciens DSM 7T. DNA–DNA hybridizations (DDH) performed with genomic DNA from strains DSM 7T and FZB42T yielded relatedness values of 63.7–71.2 %. Several methods of genomic analysis, such as direct whole-genome comparison, digital DDH and microarray-based comparative genomichybridization (M-CGH) were used as complementary tests. The group of plant-associated strains could be distinguished from strain DSM 7T and the type strain of B. subtilis by differences in the potential to synthesize non-ribosomal lipopeptides and polyketides. Based on the differences found in the marker gene sequences and the whole genomes of these strains, we propose two novel subspecies, designated B. amyloliquefaciens subsp. plantarum subsp. nov., with the type strain FZB42T ( = DSM 23117T = BGSC 10A6T), and B. amyloliquefaciens subsp. amyloliquefaciens subsp. nov., with the type strain DSM 7T( = ATCC 23350T = Fukumoto Strain FT), for plant-associated and non-plant-associated representatives, respecitvely. This is in agreement with results of DDH and M-CGH tests and the MALDI-TOF MS of cellular components, all of which suggested that the ecovars represent two different subspecies.

APA, Harvard, Vancouver, ISO, and other styles

13

Zhang, Qiu Qin, Jie Shi, Pei Yao Shen, Fei Xi, Cheng Yu Qian, Guo Hua Zhang, Hai Jun Zhu, and Hong Mei Xiao. "Exploring the Efficacy of Biocontrol Microbes against the Fungal Pathogen Botryosphaeria dothidea JNHT01 Isolated from Fresh Walnut Fruit." Foods 11, no. 22 (November 15, 2022): 3651. http://dx.doi.org/10.3390/foods11223651.

Full text

Abstract:

Biological control by antagonistic microorganisms are an effective and environmentally friendly approach in postharvest disease management. In order to develop a biocontrol agent for fresh walnut fruit preservation, the potential biocontrol effects of Bacillus amyloliquefaciens RD.006 and Hanseniaspora uvarum FA.006 against the main fungal pathogen of walnuts were evaluated. Botryosphaeria species showed the highest detection, and the JNHT01 strain showed the strongest pathogenicity. Bot. dothidea JNHT01 caused gray mold and brown rot on fresh walnuts, and its incidence rate reached 100% after an 8 days incubation. The growth of this fungal strain can be promoted by lighting, with a maximum growth rate achieved at a pH of 7 and at 28 °C. B. amyloliquefaciens RD.006 and H. uvarum FA.006 supernatants at a concentration of 1–15% v/v showed antifungal activity. The mycelial growth inhibition rates of Bot. dothidea JNHT01 were 23.67–82.61% for B. amyloliquefaciens RD.006 and 1.45–21.74% for H. uvarum FA.006. During Bot. dothidea JNHT01 growth, the biomass, nucleic acid leakage, and malondialdehyde content gradually increased, while the DPPH scavenging capacity and SOD activity decreased. The B. amyloliquefaciens RD.006 and H. uvarum FA.006 strains showed antifungal activity by damaging fungal cell membranes and reducing fungal antioxidant activity. Moreover, the antifungal effect of B. amyloliquefaciens RD.006 was higher than that of H. uvarum FA.006. Hence, the RD.006 strain of B. amyloliquefaciens can be considered a potential biocontrol agent for the management of postharvest walnut diseases caused by Bot. dothidea.

APA, Harvard, Vancouver, ISO, and other styles

14

Hashmi, Sidra, Sajid Iqbal, Iftikhar Ahmed, and Hussnain Ahmed Janjua. "Production, Optimization, and Partial Purification of Alkali-Thermotolerant Proteases from Newly Isolated Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12." Processes 10, no. 6 (May 25, 2022): 1050. http://dx.doi.org/10.3390/pr10061050.

Full text

Abstract:

Proteases that can remain active under extreme conditions such as high temperature, pH, and salt concentration are widely applicable in the commercial sector. The majority of the proteases are rendered useless under harsh conditions in industries. Therefore, there is a need to search for new proteases that can tolerate and function in harsh conditions, thus improving their commercial value. In this study, 142 bacterial isolates were isolated from diverse alkaline soil habitats. The two highest protease-producing bacterial isolates were identified as Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12, respectively, based on 16S rRNA sequencing. Optimal protease production was detected at pH 8, 37 °C, 48 h, 5% (w/v) NaCl for Bacillus subtilis S1 (99.8 U/mL) and pH 9, 37 °C, 72 h, 10% (w/v) NaCl for Bacillus amyloliquefaciens KSM12 (94.6 U/mL). The molecular weight of these partially purified proteases was then assessed on SDS-PAGE (17 kDa for Bacillus subtilis S1 and 65 kDa for Bacillus amyloliquefaciens KSM12), respectively. The maximum protease activity for Bacillus subtilis S1 was detected at pH 8, 40 °C, and for Bacillus amyloliquefaciens KSM12 at pH 9, 60 °C. These results suggest that the proteases secreted by Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12 are suitable for industries working in a highly alkaline environment.

APA, Harvard, Vancouver, ISO, and other styles

15

Байдалинов, А. И., О. Н. Шемшура, Э. Т. Исмаилова, Г. Т. Джакибаева, Д. А. Тлеубекова, Г. Б. Баймаханова, and Ш. Т. Кенжеев. "Оценка биобезопасности штамма Bacillus amyloliquefaciens МВ40, перспективного в качестве основы биопрепарата против бактериального ожога плодовых культур." МИКРОБИОЛОГИЯ ЖӘНЕ ВИРУСОЛОГИЯ, no. 4(35) (March 16, 2022): 38–47. http://dx.doi.org/10.53729/mv-as.2021.04.03.

Full text

Abstract:

Проведена оценка факторов патогенности(токсичности) и аллергенности штамма Bacillus amyloliquefaciens МВ40, перспективного в борьбе с бактериальным ожогом. Установлено, что штамм бактерии Bacillus amyloliquefaciens МВ40 не является патогенным, относится к 4-му классу опасности, и может быть использован в качестве основы для создания биопрепарата против бактериального ожога плодовых культур.

APA, Harvard, Vancouver, ISO, and other styles

16

Adhipathi, P., S. Nakkeeran, P. Renuka Devi, R. Velazhahan, and T. Raguchander. "PGPR Induced Differencial Expression of Defence Enzymes Regulating Resistance Against Colletotrichum Capsici in Turmeric." JOURNAL OF ADVANCES IN BIOTECHNOLOGY 4, no. 2 (July 30, 2014): 358–71. http://dx.doi.org/10.24297/jbt.v4i2.5002.

Full text

Abstract:

Plant growth promoting Bacillus amyloliquefaciens strain BaTNAU5 and Pseudomonas fluorescens strain Pf3TNAU were evaluated in turmeric for induction defence by differential expression of defence enzymes against Colletotrichum capsici. Further the activity and quantitative assay of the defense enzymes peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), Î²â€‘1,3-glucanase, chitinase, catalase and defense-inducing chemicals (total phenols) significantly increased with soil and foliar application of B. amyloliquefaciens (BaTNAU5) and P. fluorescens (Pf3TNAU). Western blot analysis of protein extracts from B. amyloliquefaciens (BaTNAU5) and P. fluorescens (Pf3TNAU) challenged with C. capsici,Â a chitinase with an apparent molecular weight of 28 kDa was induced four days after treatment.

APA, Harvard, Vancouver, ISO, and other styles

17

Liu, Xiaoming, Jae Min Shim, Zhuang Yao, Jae Yong Lee, Kang Wook Lee, Hyun-Jin Kim, Kyung-Sik Ham, and Jeong Hwan Kim. "Properties of Antimicrobial Substances Produced by Bacillus amyloliquefaciens CJW15 and Bacillus amyloliquefaciens SSD8." Microbiology and Biotechnology Letters 44, no. 1 (March 28, 2016): 9–18. http://dx.doi.org/10.4014/mbl.1509.09008.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Zhang, Nanchi, Li Wang, and Yong Wei. "Effects of Bacillus amyloliquefaciens and Bacillus pumilus on Rumen and Intestine Morphology and Microbiota in Weanling Jintang Black Goat." Animals 10, no. 9 (September 9, 2020): 1604. http://dx.doi.org/10.3390/ani10091604.

Full text

Abstract:

The importance of Bacillus as feed additives in animals’ production is well recognized. Bacillus amyloliquefaciens and Bacillus pumilus are involved in promoting animal growth performance and immunological indicators. However, their precise roles in the modulation of microbiota and immune response in goat rumen and intestines have not been investigated. The aim of the current work was to evaluate the impacts of Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09 in the development of rumen and small intestinal and microbial communities in rumen and caecum of weanling Jintang black goats. Morphological alterations of rumen and small intestine (duodenum, jejunum, and ileum) were evaluated by histochemical staining, and ruminal contents and cecal feces were analyzed by 16S rRNA sequencing in an Illumina NovaSeq platform. Morphological analysis showed that feeding weanling goats with Bacillus amyloliquefaciens fsznc-06 or Bacillus pumilus fsznc-09 enhanced ruminal papilla and small intestinal villus growth. In addition, 16S rRNA sequencing analysis indicated that microbial richness and diversity (Shannon, Simpson, Chao1, and ACE) and the relative richness of multiple or potential beneficial bacteria were higher in weaned black goats fed on Bacillus amyloliquefaciens fsznc-06 or Bacillus pumilus fsznc-09, but that of multiple or potentially pathogenic bacteria were lower, as compared with the control group. Tax4Fun analysis predicting the functional profiling of microbial communities showed that microbial communities in rumen or caecum were highly influential on metabolism and organism systems after feeding weanling goats with Bacillus amyloliquefaciens fsznc-06 or Bacillus pumilus fsznc-09. It was suggested that Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09 might be an auspicious antibiotic alternative to improve black goat growth and health by changing rumen and gut microbiota positively.

APA, Harvard, Vancouver, ISO, and other styles

19

Ahmat, Marhaba, Junhao Cheng, Zaheer Abbas, Qiang Cheng, Zhen Fan, Baseer Ahmad, Min Hou, et al. "Effects of Bacillus amyloliquefaciens LFB112 on Growth Performance, Carcass Traits, Immune, and Serum Biochemical Response in Broiler Chickens." Antibiotics 10, no. 11 (November 22, 2021): 1427. http://dx.doi.org/10.3390/antibiotics10111427.

Full text

Abstract:

This study aimed to investigate the effects of Bacillus amyloliquefaciens LFB112 on the growth performance, carcass traits, immune response, and serum biochemical parameters of broiler chickens. A total of 396 1 day old, mixed-sex commercial Ross 308 broilers with similar body weights were allotted into six treatment groups. The assigned groups were the CON group (basal diet with no supplement), AB (antibiotics) group (basal diet + 150 mg of aureomycin/kg), C+M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 powder with vegetative cells + metabolites), C group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 vegetative cell powder with removed metabolites), M group (basal diet + 5 × 108 CFU/kg B. amyloliquefaciens LFB112 metabolite powder with removed vegetative cells), and CICC group (basal diet + 5 × 108 CFU/kg Bacillus subtilis CICC 20179). Results indicated that chickens in the C+M, C, and M groups had higher body weight (BW) and average daily gain (ADG) (p < 0.05) and lower feed conversion ratio (FCR) (p = 0.02) compared to the CON group. The C+M group showed the lowest abdominal fat rate compared to those in the CON, AB, and CICC groups (p < 0.05). Compared to the CON group, serum IgA and IgG levels in the C+M, C, and M groups significantly increased while declining in the AB group (p < 0.05). B. amyloliquefaciens LFB112 supplementation significantly reduced the serum triglyceride, cholesterol, urea, and creatinine levels, while increasing the serum glucose and total protein (p < 0.05). In conclusion, B. amyloliquefaciens LFB112 significantly improved the growth performance, carcass traits, immunity, and blood chemical indices of broiler chickens and may be used as an efficient broiler feed supplement.

APA, Harvard, Vancouver, ISO, and other styles

20

Islam, Md Rayhan, Omit Kumer Mondol, Md Saimoon Rahman, Md Morsaline Billah, Mohammad Shahedur Rahman, and Umme Salma Zohora. "Screening of ?-amylase producing bacteria from tannery wastes of Hazaribag, Bangladesh." Jahangirnagar University Journal of Biological Sciences 5, no. 2 (June 11, 2017): 1–10. http://dx.doi.org/10.3329/jujbs.v5i2.32511.

Full text

Abstract:

Alpha amylases (?-amylases) are one of the most imperative enzymes for producing simple sugar units from complex sugar molecules. Attempts were made to isolate amylolytic bacterial strains from soil samples of tannery wastes collected from Hazaribagh, Dhaka and subsequent partial characterization was performed. Bacterial isolates were primarily screened for ?- amylase activity on starch agar medium. Based on microscopic and biochemical properties of isolates, ?-amylase activity of bacterial isolates were determined to find out two best producers of the enzyme. Subsequent molecular identification of these two ?-amylase producing bacterial isolates using 16s rRNA sequence analysis showed that isolates were Bacillus amyloliquefaciens and B. subtilis respectively. In submerged fermentation the B. amyloliquefaciens showed the highest activity (2.13 U/ml) while B. subtilis showed the second highest activity (1.89 U/ml). Characterization of the enzyme produced by B. amyloliquefaciens revealed that the maximum activity demonstrated at incubation time 25 min, pH 7.0 and temperature 500C. This newly isolated B. amyloliquefaciens could be exploited for the industrial production of ?-amylase with commercial implications.Jahangirnagar University J. Biol. Sci. 5(2): 1-10, 2016 (December)

APA, Harvard, Vancouver, ISO, and other styles

21

Jia, Qinlan, Yingying Fan, Shuaishuai Duan, Qiaomei Qin, Yu Ding, Min Yang, Yan Wang, Fengjuan Liu, and Cheng Wang. "Effects of Bacillus amyloliquefaciens XJ-BV2007 on Growth of Alternaria alternata and Production of Tenuazonic Acid." Toxins 15, no. 1 (January 7, 2023): 53. http://dx.doi.org/10.3390/toxins15010053.

Full text

Abstract:

Large amounts of processing tomato are grown in Xinjiang, China. Tomato black spot disease, caused by Alternaria spp., and the produced alternaria toxins in tomato products are posing risks to human health. In this study, we isolated a rhizospheric bacterium, XJ-BV2007, from tomato (Solanum lycopersicum) fields, which we identified as Bacillus amyloliquefaciens. We found that this bacterium has a strong antagonistic effect against Alternaria alternata and reduces the accumulation of alternaria toxins in tomatoes. According to the antifungal activity of the bacteria-free filtrate, we revealed that B. amyloliquefaciens XJ-BV2007 suppresses A. alternata by the production of antifungal metabolites. Combining semi-preparative high-performance liquid chromatography, we employed UPLC-QTOF-MS analysis and the Oxford cup experiment to find that fengycin plays an important role in inhibiting A. alternata. This paper firstly reported that B. amyloliquefaciens efficiently controls tomato black spot disease and mycotoxins caused by A. alternata. B. amyloliquefaciens XJ-BV2007 may provide an alternative biocontrol strain for the prevention of tomato black spot disease.

APA, Harvard, Vancouver, ISO, and other styles

22

Nechypurenko, O., M. Kharkota, and L. Avdeeva. "Safety of carotene-producing strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 for homoiothermal animals." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 68, no. 3 (2014): 21–24. http://dx.doi.org/10.17721/1728_2748.2014.68.21-24.

Full text

Abstract:

It was detected that the strains of carotenoid-producing Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 are safe for warm-blooded animals, also avirulent and do not show toxic and toxigenic effect. It was determined the absence of the ability of strains of Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 to produce putrescine and cadaverine. In experiments on mice was shown that oral and intraperitoneal administration of suspensions of the strains of bacilli, containing 15 billion cells/mouse did not cause the death of experimental animals and did not lead to pathologic changes in the tissues of their bodies. Thus, strains Bacillus sp. 1.1 and B. amyloliquefaciens UCM B-5113 are non-pathogenic and safe for warm-blooded animals.

APA, Harvard, Vancouver, ISO, and other styles

23

Vehmaanperä, Jari, Gerhard Steinborn, and Jürgen Hofemeister. "Genetic manipulation of Bacillus amyloliquefaciens." Journal of Biotechnology 19, no. 2-3 (July 1991): 221–40. http://dx.doi.org/10.1016/0168-1656(91)90061-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Wong, J. H., J. Hao, Z. Cao, M. Qiao, H. Xu, Y. Bai, and T. B. Ng. "An antifungal protein fromBacillus amyloliquefaciens." Journal of Applied Microbiology 105, no. 6 (December 2008): 1888–98. http://dx.doi.org/10.1111/j.1365-2672.2008.03917.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Kochhar, Sunil, and Ramji D. Dua. "Thermostable liquefying ?-amylase fromBacillus amyloliquefaciens." Biotechnology Letters 12, no. 5 (May 1990): 393–96. http://dx.doi.org/10.1007/bf01024438.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Wang, Junhua, Jiying Qiu, Xiaoyu Yang, Jinyu Yang, Shuangzhi Zhao, Qingxin Zhou, and Leilei Chen. "Identification of Lipopeptide Iturin A Produced by Bacillus amyloliquefaciens NCPSJ7 and Its Antifungal Activities against Fusarium oxysporum f. sp. niveum." Foods 11, no. 19 (September 26, 2022): 2996. http://dx.doi.org/10.3390/foods11192996.

Full text

Abstract:

Bacillus amyloliquefaciens NCPSJ7 showed potential fungicidal activities for the effective control of fungal infection. From the PCR test, the key genes (srfAA, sfp, fenD, bmyB, ituD, and ituC) were detected in B. amyloliquefaciens NCPSJ7. These genes were closely related to the lipopeptides (LPs) synthesis. Next, three LPs families were identified with liquid chromatography–mass spectrometry (LC/MS), including iturin A, fengycin A, and surfactin. After purification with C18, the main active antifungal compound was proven to be C14-iturin A by ESI-HRMS, which has significant activities against fungi. These results proved that C14-iturin A played an important role in inhibiting the growth of fungi for B. amyloliquefaciens NCPSJ7. Furthermore, the isolated LP could inhibit mycelial growth and conidia germination at 30 μg/mL. SEM allowed us to observe that mycelial morphology and conidia germination were also affected. The mycelial ultrastructure TEM observations showed that the external electron-dense outer layer cell wall, which mainly consisted of glycoproteins, was affected. Furthermore, swollen mitochondria, enriched glycogen, and increased vacuoles were also found. LP also affected the intact wall and membranes, leading to their increased permeability, which was proved by propidium iodide (PI) staining and conductivity measurements. Meanwhile, the ergosterol, which has an affinity for iturin A, also increased. These results indicated that LP caused fungal dysfunction and membrane permeability increase, leading to fungal inhibition. Identifying and studying LPs is important in exploring the fungicidal activities of B. amyloliquefaciens, which promotes the use of B. amyloliquefaciens NCPSJ7 as a potential candidate for biocontrol.

APA, Harvard, Vancouver, ISO, and other styles

27

Shahzad, Raheem, Adeeb Shehzad, Saqib Bilal, and In-Jung Lee. "Bacillus amyloliquefaciens RWL-1 as a New Potential Strain for Augmenting Biochemical and Nutritional Composition of Fermented Soybean." Molecules 25, no. 10 (May 18, 2020): 2346. http://dx.doi.org/10.3390/molecules25102346.

Full text

Abstract:

Soybean (Glycine max L.) is a good source of natural antioxidants and commonly consumed as fermented products such as cheonggukjang, miso, tempeh, and sufu in Asian countries. The aim of the current study was to examine the influence of novel endophytic bacterial strain, Bacillus amyloliquefaciens RWL-1 as a starter for soybean fermentation. During fermentation, the cooked soybeans were inoculated with different concentrations (1%, 3%, and 5%) of B. amyloliquefaciens RWL-1. The changes in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities, total phenolic contents, isoflavones (Daidzin, Genistin, Glycitin, Daidzein, Glycitein, and Genistein), amino acids (aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, and proline) composition, and minerals (calcium, copper, iron, potassium, magnesium, manganese, sodium, nickel, lead, arsenic, and zinc) were investigated. The level of antioxidants, total phenolic contents, isoflavones, and total amino acids were higher in fermented soybean inoculated with 1% B. amyloliquefaciens RWL-1 after 60 h of fermentation as compared to control, 3% and 5% B. amyloliquefaciens RWL-1. Additionally, fermented soybean inoculated with 5% B. amyloliquefaciens RWL-1 showed the highest values for mineral contents. Changes in antioxidant activities and bioactive compounds depended on the concentration of the strain used for fermentation. From these results, we conclude that fermented soybean has strong antioxidant activity, probably due to its increased total phenolic contents and aglycone isoflavone that resulted from fermentation. Such natural antioxidants could be used in drug and food industries and can be considered to alleviate oxidative stress.

APA, Harvard, Vancouver, ISO, and other styles

28

Jiang, Jian. "The Mechanism of Oxidative Stress in Cells Isolation, Identification, and Genome-Wide Sequence Analysis of Nitrite Amylolytic Bacillus." Stem Cells International 2022 (October 14, 2022): 1–9. http://dx.doi.org/10.1155/2022/9521900.

Full text

Abstract:

To improve the quality of traditional fermented pickles and reduce the nitrite content in the production process of pickles, the target bacteria for efficient nitrite degradation were screened from traditional fermented pickles. Pickles (picked vegetables), a traditional dish favored by many Chinese, are mildly salted and lactic acid-fermented vegetables in China. However, the presence of nitrite in pickles is a bottleneck which limits further development of the pickle industry. More attention is drawn to the problem of the presence of nitrite in pickles. Having harmful effect in the acidic environment produced by gastric acid, nitrite is converted into carcinogenic nitrosamine. After screening several nitrite-degrading bacteria in the early stage, a Gram-positive round ended Bacillus amyloliquefaciens is named as Bacillus amyloliquefaciens JBA-CH9, which can degrade nitrite efficiently. Bacillus amyloliquefaciens is a common bacterium in the food fermentation industry. Then, the optimum conditions for nitrite degradation of the strain were explored according to the inoculation amount, temperature and salinity, and the whole genome of Bacillus amyloliquefaciens JBA-CH9 was sequenced. The results showed that the strain had the best degradation effect on nitrite under the conditions of inoculation amount of 9%, salinity of 5%, and 30°C, and the highest degradation rate of nitrite was 91.47%. The results of whole genome sequencing showed that the strain had a large number of functional genes related to amino acids, carbohydrates, and lipids and contained nitrite reductase genes related to nitrite metabolism. Therefore, Bacillus amyloliquefaciens JBA-CH9 is a functional strain that can degrade nitrite efficiently.

APA, Harvard, Vancouver, ISO, and other styles

29

NAKHAYEVA, N. V., K. V. KANTOR, T. V. ROMANOVSKAYA, N. V. SVERCHKOVA, and E. I. KАLАMIYETS. "SCREENING AND OPTIMIZATION OF SUBMERGED FERMENTATION CONDITION FOR PROBIOTIC BACTERIA BACILLUS AMYLOLIQUEFACIENS AS THE BASIC OF FEED ADDITIVE BIODIGESTIN-C." Микробные биотехнологии: фундаментальные и прикладные аспекты 13 (October 21, 2021): 192–203. http://dx.doi.org/10.47612/2226-3136-2021-13-192-203.

Full text

Abstract:

Viability of isolated sporulating bacteria showing complex enzymatic and antimicrobial activity was evaluated under conditions simulating gastrointestinal tract of animals and strains B. amyloliquefaciens BIM В-1513G and B. amyloliquefaciens BIM В-1510G displaying resistance to low pH values, presence of bile and pepsin were selected. Submerged fermentation parameters (temperature, aeration rate) were optimized for selected probiotic strains to promote their growth, antimicrobial and enzymatic activities.

APA, Harvard, Vancouver, ISO, and other styles

30

Hong, Yuxuan, Yang Cheng, Leluo Guan, Zutao Zhou, Xiaowen Li, Deshi Shi, and Yuncai Xiao. "Bacillus amyloliquefaciens TL Downregulates the Ileal Expression of Genes Involved in Immune Responses in Broiler Chickens to Improve Growth Performance." Microorganisms 9, no. 2 (February 13, 2021): 382. http://dx.doi.org/10.3390/microorganisms9020382.

Full text

Abstract:

Bacillus amyloliquefaciens TL promotes broiler chicken performance by improving nutrient absorption and utilization and reducing intestinal inflammation. In this study, RNA-sequencing (RNA-seq)-based transcriptomes of ileal tissues collected from probiotic-fed and control broiler chickens were analyzed to elucidate the effects of the probiotic B. amyloliquefaciens TL, as a feed additive, on the gut immune function. In total, 475 genes were significantly differentially expressed between the ileum of probiotic-fed and control birds. The expression of genes encoding pyruvate kinase, prothymosin-α, and heat stress proteins was high in the ileum of probiotic-fed birds (FPKM > 500), but not in the control group. The gene ontology functional enrichment and pathway enrichment analyses revealed that the uniquely expressed genes in the control group were mostly involved in immune responses, whereas those in the probiotic group were involved in fibroblast growth factor receptor signaling pathways and positive regulation of cell proliferation. Bacillus amyloliquefaciens TL downregulated the expression of certain proinflammatory factors and affected the cytokine–cytokine receptor interaction pathway. Furthermore, B. amyloliquefaciens TL in broiler diets altered the expression of genes involved in immune functions in the ileum. Thus, it might contribute to improved broiler growth by regulating the immune system and reducing intestinal damage in broilers.

APA, Harvard, Vancouver, ISO, and other styles

31

Liu, Dong, Kunyuan Li, Jiulong Hu, Weiyan Wang, Xiao Liu, and Zhimou Gao. "Biocontrol and Action Mechanism of Bacillus amyloliquefaciens and Bacillus subtilis in Soybean Phytophthora Blight." International Journal of Molecular Sciences 20, no. 12 (June 14, 2019): 2908. http://dx.doi.org/10.3390/ijms20122908.

Full text

Abstract:

With the improper application of fungicides, Phytophthora sojae begins to develop resistance to fungicides, and biological control is one of the potential ways to control it. We screened two strains of Bacillus; Bacillus amyloliquefaciens JDF3 and Bacillus subtilis RSS-1, which had an efficient inhibitory effect on P. sojae. They could inhibit mycelial growth, the germination of the cysts, and the swimming of the motile zoospores. To elucidate the response of P. sojae under the stress of B. amyloliquefaciens and B. subtilis, and the molecular mechanism of biological control, comparative transcriptome analysis was applied. Transcriptome analysis revealed that the expression gene of P. sojae showed significant changes, and a total of 1616 differentially expressed genes (DEGs) were detected. They participated in two major types of regulation, namely “specificity” regulation and “common” regulation. They might inhibit the growth of P. sojae mainly by inhibiting the activity of ribosome. A pot experiment indicated that B. amyloliquefaciens and B. subtilis enhanced the resistance of soybean to P. sojae, and their control effects of them were 70.7% and 65.5%, respectively. In addition, B. amyloliquefaciens fermentation broth could induce an active oxygen burst, NO production, callose deposition, and lignification. B. subtilis could also stimulate the systemic to develop the resistance of soybean by lignification, and phytoalexin.

APA, Harvard, Vancouver, ISO, and other styles

32

Maslova, M. V., E. V. Grosheva, A. V. Budagovsky, O. N. Budagovskaya, and I. A. Kameneva. "ANTAGONISTIC ACTIVITY OF PAENIBACILLUS POLYMYXA, BACILLUS AMYLOLIQUEFACIENS AND THEIR LASER STIMULATION AGAINST PHYTOPATHOGENS." TAURIDA HERALD OF THE AGRARIAN SCIENCES 3 (27) (2021): 135–34. http://dx.doi.org/10.33952/2542-0720-2021-3-27-125-134.

Full text

Abstract:

Nowadays, thanks to organic farming development, particular attention is paid to the biological methods of plants protection, especially from diseases. Therefore, new biological preparations creation and studying their effectiveness are promising directions. The aim of the research was to study the antagonistic activity of the bacteria Paenibacillus polymyxa and Bacillus amyloliquefaciens against some phytopathogens (Pseudomonas syringae van Hall, Fusarium oxysporum Schltdl, Alternaria alternata (Fr.) Keissl.), as well as their reaction to laser irradiation. The research was carried out in the Research Problem Laboratory “Biphotonika” of the Michurinsk State Agrarian University and the Department of Agricultural Microbiology of the Research Institute of Agriculture of Crimea in 2016–2021. The antagonistic activity of the bacteria was evaluated by the double culture method. The effectiveness of irradiation (as a result of their treatment with coherent light) was determined by the change in the number of bacterial cells in the suspension. The study of the activity of bacteria-antagonists against pathogens showed that the suppression of P. syringae, F. oxysporum, A. alternata growth in the double culture with P. polymyxa compared to the control variants was 32.5 %; 4.0 % and 77.9 %, respectively. B. amyloliquefaciens suppressed the growth of P. syringae by 25.9 %, F. oxysporum – by 49.0 %; A. alternate – by 61.1 %. An increase in the number of cells in suspensions of P. polymyxa and B. amyloliquefaciens after coherent light irradiation by 26.6 % and 36.7 %, respectively, was also found. Thus, to control P. syringae, bacterial strain of P. polymyxa P was more effective. The bacterial strain B. amyloliquefaciens 01-1 showed antagonistic activity against F. oxysporum. Both studied microorganisms were effective against A. alternata. To increase the activity of cell division of antagonist strains of phytopathogens, it is advisable to use coherent light irradiation. In future, it is advisable to test the level of antagonistic activity of the bacteria P. polymyxa and B. amyloliquefaciens after laser irradiation.

APA, Harvard, Vancouver, ISO, and other styles

33

Hong, Yuxuan, Yingxian Cheng, Yanjuan Li, Xiaowen Li, Zutao Zhou, Deshi Shi, Zili Li, and Yuncai Xiao. "Preliminary Study on the Effect of Bacillus amyloliquefaciens TL on Cecal Bacterial Community Structure of Broiler Chickens." BioMed Research International 2019 (October 3, 2019): 1–11. http://dx.doi.org/10.1155/2019/5431354.

Full text

Abstract:

Probiotics can promote the health and growth performance of animals through modulation of intestinal microbiota. When used as a feed additive, they have the potential to minimize or abolish the use of antibiotics. In this study, we investigated the effect of the probiotic strain Bacillus amyloliquefaciens TL on the growth performance and cecum microflora composition in Cobb 500 broiler chickens. In total, 180 broilers were randomly divided into three groups—each group comprised 4 pens, and each pen contained 15 chickens. The three groups were fed either a control diet, or a diet supplemented with either the antibiotic chlortetracycline or B. amyloliquefaciens TL. Broilers were weighed, and cecum contents were collected on days 7, 14, 21, and 35, respectively. The broilers in both the antibiotic and probiotic groups exhibited significant weight gain compared with controls, exhibiting increases of 16.02% and 13.40%, respectively, after 35 days (P<0.01). Similarly, the feed conversion ratio (FCR, 1–35 days) of broilers in the chlortetracycline and B. amyloliquefaciens TL groups was lower than that of the controls. HiSeq high-throughput sequencing of 16S rRNA of the cecal microbiota was performed on days 7, 14, 21, and 35, respectively. The Firmicutes/Bacteroidetes ratio was higher in the chlortetracycline and B. amyloliquefaciens TL groups than in the control group on days 14, 21, and 35, and especially on day 21. The prevalence of genera Oscillospira, Ruminococcus, Butyricicoccus, and Faecalibacterium (Firmicutes) was higher in the antibiotic and probiotic groups, while that of Bacteroides, Parabacteroides (Bacteroidetes), and Lactobacillus was higher in the control group. In this study, the changes in the microbiota of the probiotic group were similar to those in the antibiotic group. These results suggest that the probiotic strain B. amyloliquefaciens TL can modulate the cecal microbiota of broilers similar to chlortetracycline.

APA, Harvard, Vancouver, ISO, and other styles

34

Villegas-Espinoza, Jorge Arnoldo, Edgar Omar Rueda-Puente, Bernardo Murillo-Amador, María Esther Puente, Francisco Higinio Ruiz-Espinoza, Sergio Zamora-Salgado, and Félix Alfredo Beltran Morales. "Bacterias promotoras de crecimiento de plantas autóctonas y su efecto en Prosopis chilensis (Molina) Stunz." Revista Mexicana de Ciencias Agrícolas 5, no. 6 (February 13, 2018): 1041. http://dx.doi.org/10.29312/remexca.v5i6.888.

Full text

Abstract:

En la actualidad se está deforestando en México la especie de Prosopis spp. para utilizarse como leña y carbón, en diferentes zonas áridas de nuestro país. Para su producción se utiliza fertilizante sintético, con ello hay un incremento de salinidad del suelo, subsuelo y mantos acuíferos. La presente investigación se realizó en SantaAna, Sonora, México en 2006. Se aislaron y purif icaron microorganismos asociados al sistema radicular de Prosopis glandulosa que se desarrolla en cráteres de la zona volcánica de la reserva de la biosfera El Pinacate y Gran Desierto de Altar Sonora. Las bacterias promotoras de crecimiento de plantas presentan la peculiaridad de fijar el nitrógeno atmosférico; se midió el efecto de las cepas aisladas en germinación y en el desarrollo en plántulas de P. chilensis. Fueron aisladas 19 colonias; de ellas, solamente una colonia bacteriana mostró alta actividad de reducción de acetileno y capacidaddesolubilizarfosfatos,seidentificócomoBacillus amyloliquefaciens. Nuestros resultados sugieren que B. amyloliquefaciens, presenta una af inidad particular para crecer de 0 a 0.75 M de NaCl y desarrollarse en temperaturas de 30 a 50 °C. Los efectos de la inoculación de B. amyloliquefaciens, conjuntamente con A. halopraeferens, mostraron reultados favorables en el incremento de la germinación y el desarrollo de plántulas de P. chilensis. Éste es el primer reporte de B. amyloliquefaciens como bacteria promora de crecimiento de plantas asociada a P. glandulosa.

APA, Harvard, Vancouver, ISO, and other styles

35

Lu, Hedong, Hai Xu, Panping Yang, Muhammad Bilal, Shaohui Zhu, Mengyuan Zhong, Li Zhao, et al. "Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis." Genes 13, no. 6 (May 31, 2022): 984. http://dx.doi.org/10.3390/genes13060984.

Full text

Abstract:

Fengycin is a lipopeptide produced by Bacillus that has a strong inhibitory effect on filamentous fungi; however, its use is restricted due to poor production and low yield. Previous studies have shown that fengycin biosynthesis in B. amyloliquefaciens was found to be significantly increased after fructose addition. This study investigated the effect of fructose on fengycin production and its regulation mechanism in B. amyloliquefaciens by transcriptome sequencing. According to the RNA sequencing data, 458 genes were upregulated and 879 genes were downregulated. Transcriptome analysis results showed that fructose changed the transcription of amino acid synthesis, fatty acid metabolism, and energy metabolism; alterations in these metabolic pathways contribute to the synthesis of fengycin. In an MLF medium (modified Landy medium with fructose), the expression level of the fengycin operon was two-times higher than in an ML medium (modified Landy medium). After fructose was added to B. amyloliquefaciens, the fengycin-synthesis-associated genes were activated in the process of fengycin synthesis.

APA, Harvard, Vancouver, ISO, and other styles

36

Gao, Yifang, Wenhui Bian, Yuanyuan Fang, Peng Du, Xueting Liu, Xueru Zhao, and Fengjuan Li. "α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain Bacillus amyloliquefaciens SY07." Molecules 27, no. 3 (February 8, 2022): 1127. http://dx.doi.org/10.3390/molecules27031127.

Full text

Abstract:

In this work, a new strain of Bacillus amyloliquefaciens SY07 isolated from a traditional fermented soybean food was reported to possess remarkable α-glucosidase inhibitor-producing ability. Different culture media were applied for the proliferation of B. amyloliquefaciens SY07, and it was found that fermented okara broth presented the highest α-glucosidase inhibitory activity, while Luria-Bertani medium showed a negative effect. The extract from fermented okara broth acted in a dose-dependent manner to inhibit α-glucosidase activity, with an IC50 value of 0.454 mg/mL, and main inhibitors in the fermentation extract presented a reversible, uncompetitive pattern according to Lineweaver–Burk plots. Moreover, 1-deoxynojirimycin, a recognized α-glucosidase inhibitor, was found in the extract. Results indicated that B. amyloliquefaciens SY07 could utilize okara, a by-product from the soy processing industry, to generate α-glucosidase inhibitors effectively, and be regarded as a novel excellent microbial candidate for safe, economical production of potential functional foods or ingredients with hypoglycemic effect.

APA, Harvard, Vancouver, ISO, and other styles

37

Irorita Fugaban, Joanna Ivy, Jorge Enrique Vazquez Bucheli, Wilhelm Heinrich Holzapfel, and Svetoslav Dimitrov Todorov. "Bacteriocinogenic Bacillus spp. Isolated from Korean Fermented Cabbage (Kimchi)—Beneficial or Hazardous?" Fermentation 7, no. 2 (April 7, 2021): 56. http://dx.doi.org/10.3390/fermentation7020056.

Full text

Abstract:

Bacillus velezensis ST03 and ST32, Bacillus amyloliquefaciens ST06 and ST109, and Bacillus subtilis ST08 were isolated from artisanal-produced kimchi and were identified based on 16S rRNA partial sequencing. DNA obtained from the investigated bacilli generated positive results for lichenicidin, iturin, subtilosin, and surfactin on a strain-specific basis. The strains were found to produce antimicrobial metabolites with activity levels ranging between 800 and 1600 AU/mL on a strain-specific basis, as determined against Listeria monocytogenes ATCC15313. Moreover, all tested strains in this study were still active after treatment with proteolytic enzymes, even with reduced inhibition zones compared to the controls, pointing to additional antimicrobial activity possibly related to a non-proteinaceous molecular structure. Most probably these strains may express surfactin as an additional factor in their complex antimicrobial activity. B. amyloliquefaciens ST09 and B. velezensis ST03 and ST32 were characterized as positive for β-hemolysis. B. subtilis ST08 was shown to be positive for hblC and nheC and B. amyloliquefaciens ST109 for nheB. B. amyloliquefaciens ST109 generated positive results for gelatinase activity. The ability of the studied Bacillus strains to metabolize different carbohydrate sources was done based on the API50CHB test, while the enzyme production profile was recorded by the APIZym kit. All studied strains were positive producers for biogenic amines production. Studied Bacillus spp. strains were resistant to some of the evaluated antibiotics, tested according to recommendations of CLSI and EFSA.

APA, Harvard, Vancouver, ISO, and other styles

38

G. Figueroa, Mary Amor. "Bioefficacy of Bacillus amyloliquefaciens Strain DGA14 as Potential Microbial Control Agent Against Colletotrichum gloeosporioides Causing Papaya Anthracnose." International Journal of Current Microbiology and Applied Sciences 11, no. 3 (March 10, 2022): 86–94. http://dx.doi.org/10.20546/ijcmas.2022.1103.011.

Full text

Abstract:

Anthracnose is an important fungal disease of papaya and caused major losses to its production worldwide. Common management of the disease involves the use of pesticides however, due to the increasing concern on the use of pesticides, alternative control have been encouraged. Use of microbial control agents are one of the potential alternatives to the management of the disease. Hence, this study was conducted to evaluate the bioefficacy of B. amyloliquefaciens strain DGA14as potential microbial control agent against C. gloeosporioides Penz causing papaya anthracnose under in vitro and in vivo test conditions and to determine the most effective level of B. amyloliquefaciens against C. gloeosporioides. Increasing levels of the bacterium such as 15 ml, 20 ml, 25 ml, and 30 ml per liter water were tested against the pathogen and were compared to control (sterilized distilled water). The experiments were laid out in Completely Randomized Design (CRD) with three replications. The data were statistically analyzed using the Analysis of Variance (ANOVA) and the treatment means were compared using Tukey’s Honest Significant Difference (HSD). Results in in vitro test showed that Bacillus amyloliquefaciens at 30ml per liter of water significantly inhibited C. gloeosporioides with zone of growth of 13.30mm and subsequent growth inhibition of 61.75%. Results of the in vivo test likewise showed that B. amyloliquefaciens at 30ml per liter of water applied as fruit-dip delayed the appearance of anthracnose symptom by two days and significantly reduced the severity of papaya anthracnose (DSI=1.42).

APA, Harvard, Vancouver, ISO, and other styles

39

Berber, Didem, İpek Türkmenoğlu, Meral Birbir, and Nüzhet Cenk Sesal. "Efficacy of Usnea sp. Extracts in Preventing Biofilm Formation by Bacillus Species Isolated from Soaking Liquor Samples." Journal of the American Leather Chemists Association 115, no. 6 (June 1, 2020): 222–29. http://dx.doi.org/10.34314/jalca.v115i6.3820.

Full text

Abstract:

Bacteria forms biofilm to be resistant to antibacterial agents and other unfavorable environment as compared to planktonic bacterial cells. Due to resistance of bacterial biofilms to commonly used antimicrobial agents and adverse effects of these biofilms in different industries, potential natural compounds which can inhibit bacterial biofilms have attracted more attention in recent years. Lichens are known to have unique secondary metabolites with various biological activities including anti-biofilm properties. Therefore, Bacillus toyonensis, Bacillus mojavensis, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus cereus, and Bacillus licheniformis, isolated from soak liquor samples in the previous study, were tested for their ability to form biofilm in this study. Biofilm-forming Bacillus species were detected as B. subtilis, B. amyloliquefaciens, and B. velezensis. The anti-biofilm effect of the acetone extracts of Usnea sp. was evaluated at various concentrations against these biofilm-forming isolates. The anti-biofilm effect of acetone extracts of Usnea sp. against B. subtilis and B. amyloliquefaciens was observed at the concentration of 5 µg/mL by inhibition ratios of 62.75% and 72.72%, respectively. In addition, biofilm formation of B. velezensis was inhibited by the treatment with 1.25 µg/mL extracts at a 62.69% inhibition rate. Biofilm formations of B. amyloliquefaciens and B. velezensis were also suppressed by the extracts at varying percentages of inhibition ranging between 10.11-43.69% and 21.25-46.35%, respectively. This study may provide an alternative approach to overcome the biofilm formation and bacterial resistance to the antibacterial agents in the leather industry.

APA, Harvard, Vancouver, ISO, and other styles

40

SAENGSANGA, Thanakorn, Wilailak SIRIPORNADULSIL, and Surasak SIRIPORNADULSIL. "Characterization of A Lipopeptide Surfactant From Lipase-Producing Bacillus amyloliquefaciens E1PA and Its Role in Lipase-Catalyzed Oil Hydrolysis." Walailak Journal of Science and Technology (WJST) 15, no. 12 (August 24, 2017): 841–55. http://dx.doi.org/10.48048/wjst.2018.4275.

Full text

Abstract:

The lipase-catalyzed hydrolysis of fat and oil is well recognized and considered to be an important reaction in fatty acid production. Generally, lipase activity is poor in oil-water biphasic systems. In this study, surfactins were purified, identified, and molecularly characterized from 2 biosurfactant-producing strains (Bacillus amyloliquefaciens E1PA and B. subtilis TISTR 1248). A high surfactin yield was achieved from B. amyloliquefaciens E1PA culture when 4 % (v/v) glycerol was used as a carbon source. Moreover, the addition of 2 % palm oil to the fermentation broth significantly increased biosurfactant production 2-fold during culture in Mineral Salt Medium (MSM). The biosurfactant synthesized by both strains was partially categorized as a lipopeptide via Fourier transform infrared spectroscopy (FTIR) analysis. The E1PA biosurfactant was subjected to analysis of its interfacial activation in the hydrolysis reaction. The poor hydrolytic activity (12.4 %) of lipase was significantly increased (97.3 %) when surfactin (1 % v/v) was used to generate the emulsion. In addition, the gene involved in B. amyloliquefaciens E1PA surfactin biosynthesis was characterized. The surfactin (sfp) gene was PCR amplified, and then cloning and expression of recombinant sfp was performed in Escherichia coli. Thus, heterogeneous expression of sfp gene in E. coli may be used for the efficient production of active recombinant E1PA surfactin. These results suggest that the lipopeptide biosurfactant produced by B. amyloliquefaciens E1PA could be useful as a biosurfactant in the oleochemical industry to produce fatty acids and/or fatty acid ethyl esters (biodiesel)

APA, Harvard, Vancouver, ISO, and other styles

41

Ye, Miao, Linghong Sun, Ru Yang, Zaigui Wang, and KeZong Qi. "The optimization of fermentation conditions for producing cellulase of Bacillus amyloliquefaciens and its application to goose feed." Royal Society Open Science 4, no. 10 (October 2017): 171012. http://dx.doi.org/10.1098/rsos.171012.

Full text

Abstract:

The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml. The effects of each factor on producing cellulase by B. amyloliquefaciens S1 were as follows: initial pH > incubation time = culture temperature > loaded liquid volume. The optimum reaction temperature and pH were 50°C and 7.0, respectively. This enzyme is a kind of neutral cellulase that possesses resistance to heat and acidity. It showed high activity to absorbent cotton, soya bean meal and filter paper. By adding different doses of B. amyloliquefaciens S1 to the goose feed, it was found that the egg production, average egg weight, fertilization rate and the hatching rate were promoted both in experiment 1 (1.5 g kg −1 ) and experiment 2 (3 g kg −1 ). Also the difference of egg production, fertilization rate and hatching rate between experiment 1 and control group was obvious ( p < 0.05), and the average egg weight was significantly increased in experiment 2 ( p < 0.05).

APA, Harvard, Vancouver, ISO, and other styles

42

Priest, F. G., M. Goodfellow, L. A. Shute, and R. C. W. Berkeley. "Bacillus amyloliquefaciens sp. nov., nom. rev." International Journal of Systematic Bacteriology 37, no. 1 (January 1, 1987): 69–71. http://dx.doi.org/10.1099/00207713-37-1-69.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Yoo, Young J., Theodore W. Cadman, Juan Hong, and Randolph T. Hatch. "Kinetics of ?-amylase synthesis fromBacillus amyloliquefaciens." Biotechnology and Bioengineering 31, no. 4 (March 1988): 357–65. http://dx.doi.org/10.1002/bit.260310412.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Chaves, Bruno Las-Casas, Ana Paula Martinazzo, Brisabella Coca, Adriane Nunes De Souza, and Carlos Eduardo Teodoro. "Optimization of production and partial characterization of xylanase from a newly isolated Bacillus amyloliquefaciens." Ciência e Natura 42 (May 11, 2020): e9. http://dx.doi.org/10.5902/2179460x42741.

Full text

Abstract:

This paper reports the process of production optimization and partial characterization of xylanase from a newly isolated Bacillus amyloliquefacies VR002, isolated from local soil. The microorganism exhibited maximum xylanase production when 1.0% (v/v) of inoculum size was added to culture medium with initial pH 6, 1.0% (w/v) birchwood xylan, at 35 °C after 48h of incubation. Xylanase production in different carbon sources apart from birchwood xylan and xylose did not show high production levels. Optimum pH for xylanase activity was 6.0. The enzyme was alkali-stable and retained 100% of residual activity over the pH range from 6.0 to 10.0 for 24 h at 25°C. Optimum temperature for enzyme activity was 55°C. Xylanase was 100% stable at 4°C and 25°C even after 24h of incubation, a desirable characteristic for enzyme storage. Moreover, best crude extract volume and time reaction were found to be 10 µL and 5 min, respectively. After optimization of production and activity parameters, an increase of nearly 60-fold in xylanase activity (44.12 ± 4.36 U/mL) was achieved. Characteristics of B. amyloliquefaciens VR002 xylanase are particularly desirable for biotechnological applications

APA, Harvard, Vancouver, ISO, and other styles

45

Singh, Dinesh, Venkatappa Devappa, and Dhananjay Kumar Yadav. "Suppression of Tomato Bacterial Wilt Incited by Ralstonia pseudosolanacearum Using Polyketide Antibiotic-Producing Bacillus spp. Isolated from Rhizospheric Soil." Agriculture 12, no. 12 (November 25, 2022): 2009. http://dx.doi.org/10.3390/agriculture12122009.

Full text

Abstract:

Bacillus spp. has the potential to control bacterial and fungal diseases of crops. In vitro study, Bacillus amyloliquefaciens DSBA-11 showed best to inhibit the growth of Ralstonia pseudosolanacearum as compared to Bacillus cereus JHTBS-7, B. pumilus MTCC-7092, B. subtilis DTBS-5 and B. licheniformis DTBL-6.Three primers sets from nucleotide sequences of polyketide antibiotic synthase genes viz., macrolactin, difficidin and bacillaene of B. amyloliquefaciens FZB42 were designed and standardized protocol for simultaneous detection of polyketide antibiotics-producing strains of Bacillus spp. by multiplex—PCR with products size of 792 bp, 705 bp and 616 bp respectively. All the strains of B. amyloliquefaciens contained three polyketide antibiotic synthase genes, and B. subtilis possessed difficidin and macrolactin, whereas B. cereus JHTBS-7, B. pumilus MTCC-7092 and B. licheniformis DTBL-6 did not contain any polyketide antibiotic genes. By using this technique, polyketide-producing strains of Bacillus spp. were screened within a short period with high accuracy. These polyketide synthase genes were cloned by using a T&A vector to study the role of these genes in producing antibiotics that suppressed the growth of R. pseudosolanacearum under both in vitro and in vivo conditions. Bio-efficacy of cloned products of these genes macrolactin, bacillaene, and difficidin along with parent strain B. amyloliquefaciens DSBA-11 inhibited the growth of R. pseudosolanacearum and formed 1.9 cm2, 1.9 cm2, 1.7 cm2 and 3.3 cm2 inhibition area under in vitro conditions respectively. Minimum bacterial wilt disease intensity (29.3%) with the highest biocontrol efficacy (57.72%) was found in tomato cv. Pusa Ruby (susceptible to wilt disease) was treated with B. amyloliquefaciens DSBA-11 followed by cloned products of difficidin and macrolactin under glasshouse conditions. Hence, the developed multiplex protocol might be helpful for screening polyketide antibiotics producing potential strains of Bacillus spp. from soil which can apply for managing the wilt disease of tomatoes. The polyketide antibiotics produced by bacteria might have a significant role suppression of R. pseudosolanacearum due to the disintegration of cells.

APA, Harvard, Vancouver, ISO, and other styles

46

Rückert, Christian, Jochen Blom, XiaoHua Chen, Oleg Reva, and Rainer Borriss. "Genome sequence of B. amyloliquefaciens type strain DSM7T reveals differences to plant-associated B. amyloliquefaciens FZB42." Journal of Biotechnology 155, no. 1 (August 2011): 78–85. http://dx.doi.org/10.1016/j.jbiotec.2011.01.006.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Ognik, Katarzyna, Paweł Konieczka, Anna Stępniowska, and Jan Jankowski. "Oxidative and Epigenetic Changes and Gut Permeability Response in Early-Treated Chickens with Antibiotic or Probiotic." Animals 10, no. 12 (November 25, 2020): 2204. http://dx.doi.org/10.3390/ani10122204.

Full text

Abstract:

The aim of this study was to compare the effect of the use of enrofloxacin and a probiotic containing Enterococcus faecium and Bacillus amyloliquefaciens strains in the first week of life of chickens on oxidative and epigenetic changes in molecules and intestinal integrity. The three treatments were as follows: the control group received no additive in the drinking water (GC); the second group (GP) received a probiotic preparation in the drinking water during the first five days of life, providing E. faecium strain 4a1713 at 1.0 × 107 CFU/L water and B. amyloliquefaciens 4b1822 at 1.0 × 107 CFU/L water, the third group (GA) received an antibiotic (enrofloxacin 0.5 mL/L water) in the drinking water during the first five days of life. The use of both enrofloxacin and a probiotic containing E. faecium and B. amyloliquefaciens strains in chickens’ first week of life improved intestinal integrity and reduced inflammation and oxidative and epigenetic changes in the small intestine. This effect was evident both at 6 days of age and at the end of the rearing period.

APA, Harvard, Vancouver, ISO, and other styles

48

Lee, Se-Hyung, Jong-Pyo Chae, Min-Jeong Kim, and Dae-Kyung Kang. "Isolation of Bacillus amyloliquefaciens ATC6 Producing Acidic Cellulase." Journal of Animal Science and Technology 52, no. 1 (February 28, 2010): 65–70. http://dx.doi.org/10.5187/jast.2010.52.1.065.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Fu, Aikun, Qiufen Mo, Yanping Wu, Baikui Wang, Rongrong Liu, Li Tang, Zhonghua Zeng, Xiaoping Zhang, and Weifen Li. "Protective effect of Bacillus amyloliquefaciens against Salmonella via polarizing macrophages to M1 phenotype directly and to M2 depended on microbiota." Food & Function 10, no. 12 (2019): 7653–66. http://dx.doi.org/10.1039/c9fo01651a.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Cao, Shan, Qinglong Xin, Shiting Zhou, Bin Xue, Bing Liu, Fuping Lu, Yanping Wang, and Yu Li. "B. amyloliquefaciens TCCC 11319, a new Cr(iii)-tolerant bacterium for chromium-tanned leather shaving disposal." RSC Advances 7, no. 19 (2017): 11455–61. http://dx.doi.org/10.1039/c6ra27954f.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Amyloliquefaciens'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles