Relevant bibliographies by topics / Amyloliquefaciens

Academic literature on the topic 'Amyloliquefaciens'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Amyloliquefaciens"

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Zheng, Yangyang, Xudong Wang, Siyuan Liu, Kewei Zhang, Zhibo Cai, Xiuling Chen, Yao Zhang, Jiayin Liu, and Aoxue Wang. "The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato." International Journal of Molecular Sciences 19, no. 8 (July 30, 2018): 2221. http://dx.doi.org/10.3390/ijms19082221.

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To investigate whether the ech42 gene in Clonostachysrosea can improve the biocontrol efficacy of Bacillus amyloliquefaciens and its molecular mechanism. Compared to the wild type, the B. amyloliquefaciens transformed with the ech42 gene exhibited higher chitinase activity. The B. amyloliquefaciens-ech42 also showed significantly higher biocontrol efficiency compared to Botrytiscinerea when tomato plants were pre-treated with B. amyloliquefaciens-ech42. No significant difference in biocontrol efficiency was observed between the wild type and B.amyloliquefaciens-ech42 when tomato plants were first infected by Botrytiscinerea. In addition, the activity of the defense-related enzyme polyphenol oxidase, but not superoxide dismutase, was significantly higher in B. amyloliquefaciens-ech42 than in the wild type. The ech42 enhances the biocontrol efficiency of B.amyloliquefaciens by increasing the capacity of preventative/curative effects in plants, rather than by killing the pathogens.
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Ahsan, Taswar, Chaoqun Zang, Shuyi Yu, Xue Pei, Jinhui Xie, Ying Lin, Xiaozhou Liu, and Chunhao Liang. "Screening, and Optimization of Fermentation Medium to Produce Secondary Metabolites from Bacillus amyloliquefaciens, for the Biocontrol of Early Leaf Spot Disease, and Growth Promoting Effects on Peanut (Arachis hypogaea L.)." Journal of Fungi 8, no. 11 (November 20, 2022): 1223. http://dx.doi.org/10.3390/jof8111223.

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A novel Bacillus amyloliquefaciens BAM strain, with novel fermentation nutrient mediums and compositions, could produce potent antifungal secondary metabolites, as the existing strains face resistance from fungus pathogens. In the current study, we introduced two novel nutrient mediums for the fermentation process, semolina and peanut root extract, as carbon and nitrogen sources in order to maximize the antifungal effects of B. amyloliquefaciens against Cercaspora arachidichola to control early leaf spot disease in peanuts. Based on a single-factor test and the central composite design of response surface methodology, the optimum fermentation medium for Bacillus amyloliquefaciens antagonistic substance was determined, containing 15 gm/L of semolina flour, 12.5 gm/L of beef extract, and 0.5 gm/L of magnesium sulfate, which inhibited the fungal growth by 91%. In vitro, antagonistic activity showed that the fermentation broth of B. amyloliquefaciens BAM with the optimized medium formulation had an inhibition rate of (92.62 ± 2.07)% on the growth of C. arachidichola. Disease control effects in pot experiments show that the pre-infection spray of B. amyloliquefaciens BAM broth had significant efficiency of (92.00 ± 3.79)% in comparison to post-infection spray. B. amyloliquefaciens BAM broth significantly promoted peanut plant growth and physiological parameters and reduced the biotic stress of C. archidechola. Studies revealed that B. amyloliquefaciens BAM with a novel fermentation formulation could be an ideal biocontrol and biofertilizer agent and help in early disease management of early leaf spots in peanuts.
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Ranjith, Sellappan, Thangavel Kalaiselvi, Muruganagounder Muthusami, and Uthandi Sivakumar. "Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (Spodoptera frugiperda) in Maize." Microorganisms 10, no. 9 (September 16, 2022): 1850. http://dx.doi.org/10.3390/microorganisms10091850.

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Maize is an important cereal crop which is severely affected by Spodoptera frugiperda. The study aims to identify endophytic bacteria of maize root and leaf apoplastic fluid with bioprotective traits against S. frugiperda and plant growth promoting properties. Among 15 bacterial endophytic isolates, two strains—namely, RAF5 and LAF5—were selected and identified as Alcaligenes sp. MZ895490 and Bacillus amyloliquefaciens MZ895491, respectively. The bioprotective potential of B. amyloliquefaciens was evaluated through bioassays. In a no-choice bioassay, second instar larvae of S. frugiperda fed on B. amyloliquefaciens treated leaves (B+) recorded comparatively lesser growth (1.10 ± 0.19 mg mg−1 day−1) and consumptive (7.16 ± 3.48 mg mg−1 day−1) rates. In larval dip and choice bioassay, the same trend was observed. In detached leaf experiment, leaf feeding deterrence of S. frugiperda was found to be greater due to inoculation with B. amyloliquefaciens than Alcaligenes sp. The phenolics content of B. amyloliquefaciens inoculated plant was also found to be greater (3.06 ± 0.09 mg gallic acid g−1). However, plant biomass production was more in Alcaligenes sp inoculated treatment. The study thus demonstrates the potential utility of Alcaligenes sp. and B. amyloliquefaciens for improving growth and biotic (S. frugiperda) stress tolerance in maize.
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Fang, Haitian, Huiyan Liu, Ning Chen, Chenglin Zhang, Xixian Xie, and Qingyang Xu. "Site-directed mutagenesis studies on the uridine monophosphate binding sites of feedback inhibition in carbamoyl phosphate synthetase and effects on cytidine production by Bacillus amyloliquefaciens." Canadian Journal of Microbiology 59, no. 6 (June 2013): 374–79. http://dx.doi.org/10.1139/cjm-2012-0758.

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A major problem when pyrimidine de novo biosynthesis is used for cytidine production is the existence of many negative regulatory factors. Cytidine biosynthesis in Bacillus amyloliquefaciens proceeds via a pathway that is controlled by uridine monophosphate (UMP) through feedback inhibition of carbamoyl phosphate synthetase (CPS), the enzyme that converts CO2, NH3, and glutamine to carbamoyl phosphate. In this study, the gene carB encoding the large subunit of CPS from B. amyloliquefaciens CYT1 was site directed, and the UMP binding sites of feedback inhibition in Bam-CPS are described. The residues Thr-941, Thr-970, and Lys-986 in CPS from B. amyloliquefaciens were subjected to site-directed mutagenesis to alter UMP’s feedback inhibition of CPS. To find feedback-resistant B. amyloliquefaciens, the influence of the T941F, T970A, K986I, T941F/K986I, and T941F/T970A/K986I mutations on CPS enzymatic properties was studied. The recombinant B. amyloliquefaciens with mutated T941F/K986I and T941F/T970A/K986I CPS showed a 3.7- and 5.7-fold increase, respectively, in cytidine production in comparison with the control expressing wild-type CPS, which was more suitable for further application of the cytidine synthesis. To a certain extent, the 5 mutations were found to release the enzyme from UMP inhibition and to improve B. amyloliquefaciens cytidine-producing strains.
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Dunlap, Christopher A., Soo-Jin Kim, Soon-Wo Kwon, and Alejandro P. Rooney. "Phylogenomic analysis shows that Bacillus amyloliquefaciens subsp. plantarum is a later heterotypic synonym of Bacillus methylotrophicus." International Journal of Systematic and Evolutionary Microbiology 65, Pt_7 (July 1, 2015): 2104–9. http://dx.doi.org/10.1099/ijs.0.000226.

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The rhizosphere-isolated bacteria belonging to the Bacillus amyloliquefaciens subsp. plantarum and Bacillus methylotrophicus clades are an important group of strains that are used as plant growth promoters and antagonists of plant pathogens. These properties have made these strains the focus of commercial interest. Here, we present the draft genome sequence of B. methylotrophicus KACC 13105T ( = CBMB205T). Comparative genomic analysis showed only minor differences between this strain and the genome of the B. amyloliquefaciens subsp. plantarum type strain, with the genomes sharing approximately 95 % of the same genes. The results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the type strains of these two taxa are highly similar. In fact, our results show that the type strain of B. amyloliquefaciens subsp. plantarum FZB42T ( = DSM 23117T = BGSC 10A6T) does not cluster with other members of the B. amyloliquefaciens taxon. Instead, it clusters well within a clade of strains that are assigned to B. methylotrophicus, including the type strain of that species. Therefore, we propose that the subspecies B. amyloliquefaciens subsp. plantarum should be reclassified as a later heterotypic synonym of B. methylotrophicus.
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Nan, Jing, Shaoran Zhang, and Ling Jiang. "Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing." Plants 10, no. 2 (January 29, 2021): 261. http://dx.doi.org/10.3390/plants10020261.

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Citrus huanglongbing (HLB) is a destructive disease caused by Candidatus Liberibacter species and is a serious global concern for the citrus industry. To date, there is no established strategy for control of this disease. Previously, Bacillus amyloliquefaciens GJ1 was screened as the biocontrol agent against HLB. In this study, two-year-old citrus infected by Ca. L. asiaticus were treated with B. amyloliquefaciens GJ1 solution via root irrigation. In these plants, after seven irrigation treatments, the results indicated that the photosynthetic parameters, chlorophyll content, resistance-associated enzyme content and the expression of defense-related genes were significantly higher than for the plants treated with the same volume water. The content of starch and soluble sugar were significantly lower, compared to the control treatment. The parallel reaction monitoring (PRM) results revealed that treatment with B. amyloliquefaciens GJ1 solution, the expression levels of 3 proteins with photosynthetic function were upregulated in citrus leaves. The accumulation of reactive oxygen species (ROS) in citrus leaves treated with B. amyloliquefaciens GJ1 flag22 was significantly higher than untreated plants and induced the defense-related gene expression in citrus. Finally, surfactin was identified from the fermentation broth of B. amyloliquefaciens GJ1 by high-performance liquid chromatography. These results indicate that B. amyloliquefaciens GJ1 may improve the immunity of citrus by increasing the photosynthesis and enhancing the expression of the resistance-related genes.
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Wang, Da-Cheng, Chun-Hao Jiang, Li-Na Zhang, Lin Chen, Xiao-Yun Zhang, and Jian-Hua Guo. "Biofilms Positively Contribute to Bacillus amyloliquefaciens 54-induced Drought Tolerance in Tomato Plants." International Journal of Molecular Sciences 20, no. 24 (December 12, 2019): 6271. http://dx.doi.org/10.3390/ijms20246271.

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Drought stress is a major obstacle to agriculture. Although many studies have reported on plant drought tolerance achieved via genetic modification, application of plant growth-promoting rhizobacteria (PGPR) to achieve tolerance has rarely been studied. In this study, the ability of three isolates, including Bacillus amyloliquefaciens 54, from 30 potential PGPR to induce drought tolerance in tomato plants was examined via greenhouse screening. The results indicated that B. amyloliquefaciens 54 significantly enhanced drought tolerance by increasing survival rate, relative water content and root vigor. Coordinated changes were also observed in cellular defense responses, including decreased concentration of malondialdehyde and elevated concentration of antioxidant enzyme activities. Moreover, expression levels of stress-responsive genes, such as lea, tdi65, and ltpg2, increased in B. amyloliquefaciens 54-treated plants. In addition, B. amyloliquefaciens 54 induced stomatal closure through an abscisic acid-regulated pathway. Furthermore, we constructed biofilm formation mutants and determined the role of biofilm formation in B. amyloliquefaciens 54-induced drought tolerance. The results showed that biofilm-forming ability was positively correlated with plant root colonization. Moreover, plants inoculated with hyper-robust biofilm (ΔabrB and ΔywcC) mutants were better able to resist drought stress, while defective biofilm (ΔepsA-O and ΔtasA) mutants were more vulnerable to drought stress. Taken altogether, these results suggest that biofilm formation is crucial to B. amyloliquefaciens 54 root colonization and drought tolerance in tomato plants.
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Wei, Xubiao, Xiudong Liao, Jun Cai, Zhaojun Zheng, Lulu Zhang, Tingting Shang, Yu Fu, Cong Hu, Lei Ma, and Rijun Zhang. "Effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat." Animal Production Science 57, no. 9 (2017): 1899. http://dx.doi.org/10.1071/an16119.

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An experiment was conducted to investigate the effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat. 440 1-day-old Arbor Acres male broiler chicks were randomly allocated into five dietary treatments with eight replicates in a completely randomised design for 42 days. The treatments were as follows: basal diet (control) or the basal diet supplemented with 107 colony-forming units (CFU) B. amyloliquefaciens/kg (B7), 108 CFU B. amyloliquefaciens/kg (B8), 109 CFU B. amyloliquefaciens/kg (B9) or 40 mg aureomycin/kg (antibiotic). The results showed that B. amyloliquefaciens-supplemented groups had greater (P < 0.05) average daily gain during Days 1 to 21, Days 22 to 42 and Days 1 to 42, and improved (P < 0.05) feed conversion rate during Days 1 to 42 compared with the control. Broilers in the B7 and B9 groups had higher (P < 0.05) average daily feed intake than those in the control group in all experimental phases. Broilers fed either B. amyloliquefaciens or antibiotic diet showed higher (P < 0.05) a* value, b* value and lower L* value in the breast muscle than those in the control group. Dietary B. amyloliquefaciens supplementation increased (P < 0.05) the contents of C14:1, C16:1c, C18:1t, C18:1c, C20:1n-9, total monounsaturated fatty acids, C18:2c, C18:3n-3, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3, C22:6n-3 and total polyunsaturated fatty acids as well as ratio of polyunsaturated fatty acids to saturated fatty acids in breast muscle and the concentrations of C17:1, C20:1n-9, C18:2t-9, t-12, C20:2n-6, C20:3n-6, C20:3n-3, C20:4n-6, C20:5n-3 and C22:6n-3 in thigh muscle of broilers. The results indicated that B. amyloliquefaciens improved broilers’ growth performance, meat quality and fatty acid composition.
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Domenico Prisa. "Antifungal and plant growth promoting activity with Bacillus amyloliquefaciens in Aeonium subs." Magna Scientia Advanced Biology and Pharmacy 1, no. 2 (February 28, 2021): 042–50. http://dx.doi.org/10.30574/msabp.2021.1.2.0008.

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In this work, the biostimulant and biocontrol capacity of Bacillus amyloliquefaciens in succulent plants such as Aeonium was evaluated. The specific objectives were in particular: (i) Evaluate whether the use of this bacterium can increase the growth rate of Aeonium plants that typically have a slow growth cycle; (ii) evaluate if the use of B. amyloliquefaciens can lead to increased plant resistance to Fusarium oxysporum; (iii) considered if utilization of B. amyloliquefaciens allows for increased plant resistance to water and nutrient stress. The experimentation showed a significant improvement of the agronomic parameters analyzed on all Aeonium plants treated with Bacillus amyloliquefaciens. In general there was a significant increase in plant height, number of leaves, vegetative and root weight, number of new shoots. In addition, Aeonium goochiae and sunburst show a better protection against the fungus Fusarium oxysporum. The trial also highlighted the ability of B. amyloliquefaciens to guarantee a productive and qualitative production of the plants even under conditions of reduced water and nutritional resources. Plants, thanks to the interaction with soil microorganisms, can reach higher nutrient and water resources, resulting in greater resistance to abiotic stresses and better quality in the cultivation cycle.
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Pasaribu, T., E. B. Laconi, and I. P. Kompiang. "Evaluation of the nutrient contents of palm kernel cake fermented by microbial cocktails as a potential feedstuff for poultry." Journal of the Indonesian Tropical Animal Agriculture 44, no. 3 (September 24, 2019): 295. http://dx.doi.org/10.14710/jitaa.44.3.295-302.

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The aims of this research were to improve the nutritive value of palm kernel cake by fermentation technology using Bacillus amyloliquefacien, Trichoderma harzianum and cocktail microbes (combination of Bacillus amyloliquefacien and Trichoderma harzianum). Data were analyzed using Completely Randomized Design Factorial, with experiments consisting of types of microbes (B. amyloliquefaciens, T. harzianum, microbial cocktail) as treatment and incubation time (0, 3, 5, and 7 days). Parameters were crude protein and crude fiber for all treatments, and the lowest fiber analysis would continue with NDF, ADF, crude fat, and amino acids. Result showed that the three of microbes grew on palm kernel meal in third incubation and grew on and in the substrate at 7 days. microbial cocktails increased protein and reduced crude fiber better than B. amyloliquefacien and T. harzianum on palm kernel cake fermentation technology. Cocktail microbes enhanced amino acids such as methionine, arginine, and glutamic acid, also neutral detergent fiber but reduced ADF and hemicellulose. It was concluded that the palm kernel cake fermented with microbial cocktails can reduced crude fiber and increase crude protein with an incubation period of 7 days.
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Dissertations / Theses on the topic "Amyloliquefaciens"

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Makarewicz, Oliwia. "Regulation der Phytaseexpression in Bacillus amyloliquefaciens." Doctoral thesis, [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=983031339.

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Lima, Frederico Alves. "Produção de biossurfactantes por Bacillus amyloliquefaciens IT45." Universidade Federal de Uberlândia, 2017. http://dx.doi.org/10.14393/ufu.di.2017.62.

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CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Biossurfactantes são moléculas de origem microbiana que possuem importante ação na redução tensão superficial. Dentre os biossurfactantes mais efetivos estão os lipopeptídeos produzidos por bactérias do gênero Bacillus, especialmente a Surfactina. Estes biocompostos apresentam uma série de vantagens que potencializam suas aplicações, tais como: estabilidade frente a condições extremas (pH, temperatura), diversidade de estruturas químicas, excelentes propriedades superficiais e ecológicas, ação antibiótica frente a microrganismos patógenos, dentre outras. Diante deste contexto, neste trabalho foi avaliada a produção de biossurfactantes totais e a Surfactina por Bacillus amyloliquefaciens IT45, quando variada a concentração dos reagentes presentes no meio de cultivo. As fermentações submersas foram realizadas em mesa agitadora industrial e em biorreator piloto de capacidade 40 litros. Para aperfeiçoar a concentração dos reagentes presentes no meio de cultura, um Planejamento Composto Central foi desenvolvido com propósito de avaliar a influência de três variáveis (xarope de glicose, extrato de levedura e cloreto de cálcio) na tensão superficial, na produção de biossurfactantes totais e no açúcar residual. Depois das análises estatísticas, quando as variáveis estavam nas concentrações (g.L-1) de 20 para xarope de glicose, 15 para extrato de levedura e 4 para cloreto de cálcio, a tensão superficial (mN/m) foi reduzida de valores acima de 50 para 30, o açúcar residual foi mínimo e igual a 31% e a produção de biossurfactantes totais foi máxima e igual a 5,5 g.L-1, depois de um período de cultivo de 48 horas. A caracterização do biossurfactante sugeriu a presença da Surfactina e este composto foi quantificado no tempo de retenção de 13,5 minutos. Com intuito de saber a real produção de Surfactina e crescimento biomassa celular, foram feitas fermentações em biorreator piloto de 40 litros e os resultados mostraram bastantes favoráveis. O tratamento com maior destaque foi referente à receita sugerida pelo Planejamento Composto Central em que o xarope de glicose, extrato de levedura e cloreto de cálcio estavam nas concentrações (g.L-1) de 20, 15 e 4, respectivamente. Neste cultivo o crescimento celular de 6,0 x 109 CFU.mL-1, produção de Surfactina de 0,63 g.L-1 e açúcar residual de 28%. Também foi realizado teste de atividade antimicrobiana contra 5 fungos patogênicos de diferentes gêneros. O caldo fermentado livre de células mostrou-se promissor, pois causou inibição em 4 fungos dos 5 estudos. Portanto, os resultados demonstram que o Bacillus amyloliquefaciens IT45 tem potencial para produção de biocompostos, uma vez que não necessita de altas concentrações de fonte de carbono e nitrogênio para seu desenvolvimento.
Biosurfactants are molecules of microbial origin that have superficial action. Among the most effective are the lipopeptide biosurfactants produced by Bacillus, especially surfactin. These biological products have a number of advantages that potentiate their applications, such as: stability to extreme conditions (pH, temperature), diversity of chemical structures, excellent surface and ecological properties, antibiotic action against pathogenic microorganisms, etc. In this context, the productions of total biosurfactants and Surfactin by Bacillus amyloliquefaciens IT45 were evaluated when the reagents concentration present in the culture medium varied. The submerged fermentations were carried out in an industrial shaker and in a pilot bioreactor of 40 liters capacity. In order to improve the reactants concentration, a Central Composite Design was developed to evaluate the influence of three variables (glucose syrup – Glucodry, yeast extract and calcium chloride) on superficial tension, total biosurfactant production and residual sugar. After statistical analyzes, when the variables were in the concentrations (g.L-1) of 20 for Glucodry, 15 for yeast extract and 4 for calcium chloride, the superficial tension (mN/m) reduces values above 50 to about 30, the residual sugar was minimal, around 31% and the total biosurfactant production was maximum, around 5.5 gL-1, after a period of 48 hours. The characterization of the biosurfactant identified Surfactin presence that was quantified in the retention time of 13.5 minutes. In order to know the real production of Surfactin and cellular biomass growth, fermentations were made in a 40 liter pilot bioreactor and the results were quite favorable. The most important culture medium suggested by the Central Composite Design, where glucose syrup, yeast extract and calcium chloride were in the concentrations (g.L-1) of 20, 15 and 4, respectively. For this fermentation, the cellular growth was 6.0 x 109 CFU.mL-1, Surfactin production was 0.63 g.L-1 and residual sugar was 28%. The results demonstrate that Bacillus amyloliquefaciens IT45 has potential to produce biosurfactants and does not require high concentrations of carbon and nitrogen sources for its development.
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Chu, Hoang Ha. "Identification and functions of type I signal peptidases of Bacillus amyloliquefaciens." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964358999.

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Bashir, Abdallah. "Charakterisierung der Rolle von genereller Stressantwort und Sporulation bei der Anpassung von Bacillus-Stämmen an den Standort Boden." [S.l. : s.n.], 2004. http://archiv.ub.uni-marburg.de/diss/z2004/0512/.

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Mattapally, Peter Vijay. "Characterizing Bacillus amyloliquefaciens UCMB5113 on a Plant Model Arabidopsis thaliana." Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-38378.

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Organic farming is gaining importance and acceptance worldwide due to its beneficial effects in agriculture and standing against losses caused by chemical fertilizers, pesticides and fungicides. Plant growth promoting bacteria (PGPB) plays an important role in organic farming by fixing atmospheric nitrogen, chelate iron, solubilizing phosphorous, producing and modulating phytohormones, providing antibiotics against pathogens. Understanding interaction mechanisms between PGPB and plant will be helpful in developing new formulations to form a strong symbiotic relationship between plant and bacteria. Bacillus amyloliquefaciens UCMB5113 is a red pigmented, rod shaped Gram positive bacteria which has been isolated from fields of the Ukraine. In the present study UCMB5113 and its interactions with the plant has been characterized. There was a significant promotion of plant root growth and protection against biotic stress with the application of 10 μl of 1x107/ml CFU UCMB5113 culture in Arabidopsis. The UCMB5113 can significantly withstand plant antimicrobial activity to stimulate plant root growth, but needs root hair defective RHD proteins to stimulate root hair elongation. UCMB5113 has significantly inhibited primary root elongation and developed number of lateral roots and root hairs in ethylene over expressed mutant, which suggests that it may be affecting ethylene signaling pathway in plants. UCMB5113 has a distinct red pigmentation which is a 38.5kDa water soluble protein with maximum absorbance at 422nm. These features are similar to the Orange Carotenoid Protein (OCP) of Synechocystis PCC 6803. This red pigmented protein has no significant effect on plant root growth promotion. Further biochemical and molecular studies are required to characterize and confirm the mechanisms of interaction.
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Bonelli, Raquel Regina. "Extração e caracterização de uma substância antimicrobiana produzida por bacillus amyloliquefaciens." Florianópolis, SC, 2001. http://repositorio.ufsc.br/xmlui/handle/123456789/80287.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias.
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Extraiu-se uma substância antimicrobiana de uma cultura 18-24 h de Bacillus amyloliquefaciens em Caldo Triptona de Soja suplementado com Extrato de Levedura a 0,6% (TSB-YE - OXOID) por precipitação com 20% de saturação de sulfato de amônio, ressuspensão em tampão fosfato pH 7,2, diálise em membrana 3,5kD (Spectra/Por) e esterilização por filtração (membrana 0,22mm - Millipore). Esta substância foi caracterizada segundo sua sensibilidade a temperatura (50, 75 e 100°C) e pH (2,0 a 9,0). Para determinação de sua natureza química empregaram-se as enzimas (Sigma) protease de S. griseus (P6911), protease de A. saitoi (P2143), a-quimotripsina (C4129) e pepsina (P6887) a 50mg/mL e a a-amilase Termamyl 120 L (Novo Nordisk) a 1% v/v. Para verificação da manutenção da atividade após tais ensaios, o extrato foi aplicado em poços preparados em placas de Ágar Triptona de Soja suplementado com Extrato de Levedura a 0,6% (TSA-YE - OXOID) pré-semeadas com 0,1 mL de uma cultura 105 UFC/mL de Listeria monocytogenes. Determinou-se sua concentração mínima inibitória (CMI) sobre uma cultura 104 UFC/mL de L. monocytogenes e seu peso molecular por eletroforese em gel de poliacrilamida (SDS-PAGE). Como resultado dos testes de caracterização, a substância apresentou-se estável a 50°C, perdendo sua atividade gradualmente a 75°C e rapidamente a 100°C. Também foi estável em valores de pH de 2,0 a 9,0. Perdeu parcialmente sua atividade quando tratada com as enzimas protease de S.griseus, protease de A. saitoi e a-amilase, indicando uma estrutura glicoproteica. A CMI foi 25% v/v e a substância agiu de forma bacteriostática. A eletroforese indicou um peso molecular inferior a 6.500 Daltons.
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Schulz, Denys. "Avaliação toxicológica do extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10." Florianópolis, SC, 2008. http://repositorio.ufsc.br/xmlui/handle/123456789/91977.

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Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-Graduação em Ciência dos Alimentos.
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Alguns microrganismos possuem a capacidade de produzir substâncias que podem influenciar no desenvolvimento de outros microrganismos. Desde os anos 50 é relatada a capacidade de várias espécies de bactérias do gênero Bacillus de produzir substâncias com atividade antimicrobiana como peptídeos, também denominados de bacteriocinas e enzimas como a subtilisina, subtilina, as proteases e as termolisinas. Muitos desses peptídeos e enzimas têm sido caracterizados bioquimicamente e geneticamente. Embora sejam conhecidas, a função estrutural, a biossíntese e modo de ação de alguns peptídeos antimicrobianos e enzimas, muitos aspectos desses compostos ainda permanecem desconhecidos. Frente a essa realidade, o presente estudo investigou a atividade antibacteriana e antifúngica e a toxicidade in vitro e in vivo do extrato bruto de Bacillus amyloliquefaciens R 10. O extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 foi obtido por técnicas de precipitação de proteínas, centrifugação, diálise e esterilização por filtração. A padronização das análises toxicológicas e de atividade antibacteriana e antifúngica foi realizada pela determinação da concentração de proteínas do extrato bruto de Bacillus amyloliquefaciens R 10. O ensaio de atividade antibacteriana e antifúngica foi realizado pela técnica de difusão em poços. Dentre os diferentes indicadores utilizaram-se bactérias Gram-positivas (Listeria monocytogenes NCTC 098630, Staphylococcus aureus ATCC 25923 e Enterococcus faecalis ATCC 29212), Gram-negativas (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Enterobacter aerogenes ATCC 13048 e Pseudomonas aeruginosa ATCC 9027), fungos (Aspergillus fumigatus ATCC 9197 e Penicillium commune J 238) e leveduras (Rhodotorula muscilaginosa J 350, Pichia anomala DSM 70255 e Kluveromyces marxianus ATCC 16045). O extrato bruto foi testado nas concentrações de 5, 20, 40, 60 e 80 g de proteínas, em ambos os ensaios de atividade antimicrobiana. Para avaliar a toxicidade do extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 foram utilizados ensaios in vitro (genotoxicidade) e in vivo (toxicidade aguda em ratos e camundongos e toxicidade de doses repetidas em camundongos). A investigação da genotoxicidade com células VERO ATCC-CCL 81 foi realizada pelo ensaio Cometa, utilizando-se 60, 80 e 100 g de proteínas do extrato bruto. Para análise toxicológica aguda em ratos Wistar (Rattus norvegicus), utilizou-se um grupo de 10 animais (5 machos e 5 fêmeas) tratado com o extrato bruto, por gavagem oral, com a dose de 2.000µg/kg (dose máxima recomendada). Os animais foram pesados no início e no final do experimento e observados por 14 dias quanto aos possíveis sinais de toxicidade (morte, coma, convulsão, prostração, ataxia, tremores, alteração na pele ou pêlo, alteração nas mucosas, diarréia e salivação). Para análise toxicológica aguda em camundongos Swiss (Mus musculus), utilizou-se o extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 por gavagem oral, numa única administração, nas doses de 5, 50, 500 e 5.000 g/kg a 80 camundongos (40 machos e 40 fêmeas, contendo 10 animais por grupo). Outros 20 camundongos (10 machos e 10 fêmeas) receberam veículo (salina, NaCl 0,9%), pela mesma via. Para análise toxicológica de doses repetidas em camundongos Swiss (Mus musculus) utilizou-se o extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 administrado por gavagem oral, por um período de 90 dias nas doses de 50, 500 e 5.000 g/kg em 120 animais (60 machos e 60 fêmeas, contendo 20 animais por grupo). Outros 40 camundongos (20 machos e 20 fêmeas) receberam veículo (salina, NaCl 0,9%), pela mesma via. Constatou-se nos ensaios de atividade antimicrobiana que o extrato bruto de Bacillus amyloliquefaciens R 10 não apresentou atividade contra os bolores e as leveduras nas concentrações testadas, e somente apresentou atividade antibacteriana frente Listeria monocytogenes NCTC 098630. O extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 não foi genotóxico para células VERO na concentração de 60µg/ml, porém apresentou genotoxicidade nas concentrações de 80 e 100µg/ml. Na análise toxicológica aguda em ratos a dose letal 50 (DL50) foi superior a 2.000µg/kg. Na análise toxicológica aguda em camundongos, o extrato bruto apresentou boa tolerabilidade e reduzidos efeitos tóxicos agudos importantes, com DL50 superior a 5.000µg/kg. Já na análise toxicológica de doses repetidas, a non toxic adverse effect level (NOAEL) para o extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 situa-se entre as doses de 50 e 500 g/kg. Os resultados indicam que o extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 apresenta um grande potencial como conservador natural de alimentos, dada sua marcante atividade antilisterial e sua relativa segurança toxicológica. Some microorganisms are able to produce substances that can influence the growth of other microorganisms. The ability of various bacterial species of the genus Bacillus to produce substances with antimicrobial activity such as peptides, also called bacteriocins, and enzymes such as subtilisin, subtilin, proteases and thermolysins has been reported since the 1950's. Many of these peptides and enzymes have been characterized biochemically and genetically. Although the structural function, biosynthesis and mode of action of some antimicrobial peptides and enzymes have been identified, many aspects of these compounds are still unknown. The present study investigated the antibacterial and antifungal activity and the in vitro and in vivo toxicity of the crude extract of Bacillus amyloliquefaciens R 10. The antibacterial crude extract of Bacillus amyloliquefaciens R 10 was obtained by techniques of protein precipitation, centrifugation, dialysis and sterilization through filtration. The standardization of the toxicological analysis, and antibacterial and antifungal activity was realized by determining the concentration of proteins in the crude extract of Bacillus amyloliquefaciens R 10. The assay of antibacterial and antifungal activity was done by agar diffusion technique in wells. Among the different indicators were used the Gram-positive bacteria (Listeria monocytogenes NCTC 098630, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Enterobacter aerogenes ATCC 13048 and Pseudomonas aeruginosa ATCC 9027), molds (Aspergillus fumigatus ATCC 9197 and Penicillium commune J 238), and yeasts (Rhodotorula muscilaginosa J 350, Pichia anomala DSM 70255 and Kluveromyces marxianus ATCC 16045). The crude extract was tested in concentrations of 5, 20, 40, 60 and 80 g of protein in both assays of antimicrobial activity. To evaluate the Bacillus amyloliquefaciens R 10 antibacterial crude extract's toxicity were used in vitro (genotoxicity) and in vivo (acute toxicity in rats and mice and repeated doses toxicity in mice) assays. The research of the genotoxicity with VERO ATCC-CCL 81 cells was made by Comet assay, using 60, 80 e 100 g of protein of the crude extract. To the acute toxicological analysis in rats Wistar (Rattus norvegicus), were used one group with 10 animals (5 males and 5 females) treated with the crude extract by oral gavage, with dose of 2,000µg/kg (maximum dose recommended). The animals were weighed at the beginning and at the end of the experiment, and observed during 14 days as the possible toxicity signs (death, coma, convulsions, prostration, ataxy, tremors, skin or hair alterations, mucous alteration, diarrhea and salivation). To the acute toxicological analysis in Swiss mice (Mus musculus), the antibacterial crude extract of Bacillus amyloliquefaciens R 10 was used by oral gavage, in a single administration, in doses of 5, 50, 500 and 5,000 g/kg to 80 mice (40 males and 40 females, with 10 animals per group). Other 20 mices (10 males and 10 females) received the vehicle (saline, NaCl 0.9%), through the same route. To the repeated doses toxicological analysis in Swiss mice (Mus musculus) the antibacterial crude extract of Bacillus amyloliquefaciens R 10 was administrated by oral gavage during 90 days in doses of 50, 500 and 5,000 g/kg in 120 animals (60 males and 60 females, in groups of 20 animals). Other 40 mice (20 males and 20 females) received the vehicle (saline, NaCl 0.9%), through the same route. It was verified in the antimicrobial activity assays that the crude extract of Bacillus amyloliquefaciens R 10 didn't present activity against molds and yeasts in the tested concentrations, and only presented antibacterial activity against Listeria monocytogenes NCTC 098630. The antibacterial crude extract of Bacillus amyloliquefaciens R 10 was not genotoxic to VERO cells at concentration of 60µg/ml, but showed genotoxicity at concentration of 80 and 100µg/ml. In the acute toxicological analysis in rats the letal dose 50 (DL50) was higher than 2,000µg/kg. In the acute toxicological analysis in mice, the crude extract presented good tolerability and litle important acute toxic effects with DL50 higher than 5,000µg/kg. In the repeated doses toxicological analysis, a non toxic adverse effect level (NOAEL) for the antibacterial crude extract is between the doses of 50 and 500 g/kg. The results showed that the antibacterial crude extract of Bacillus amyloliquefaciens R 10 presents a great potential as a natural food preservative, due to its notable antilisterial activity, and its relative toxicological security.
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Scholz, Romy. "Synthese der Bacteriocine Amylocyclicin A und Plantazolicin in Bacillus amyloliquefaciens FZB42." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16283.

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Bacillus amyloliquefaciens FZB42 ist ein grampositives Bodenbakterium. Es kann in der Rhizosphäre das Wachstum von Pflanzen fördern und durch die Produktion von Sekundärmetaboliten phytopathogene Organismen hemmen. Aus der Genomanalyse und den dazugehörigen Arbeiten war bekannt, dass Bacillus amyloliquefaciens FZB42 nicht-ribosomal je drei antimikrobielle Polyketide und Lipopeptide herstellt, sowie zwei Siderophore und das Dipeptid Bacilysin. Für Bacillus typische Lantibiotika oder große Bacteriocine wurden nicht gefunden. In dieser Arbeit wird erstmalig gezeigt, dass Bacillus amyloliquefaciens FZB42 auf ribosomale Weise antibakterielle Peptide herstellt. Zwei bisher unbekannte Bacteriocine, Amylocyclicin A und Plantazolicin, und deren dazugehörigen Gencluster konnten identifiziert und charakterisiert werden. Amylocyclicin A ist ein unmodifiziertes Peptid, dessen N- und C-Terminus kovalent verbunden sind. Es wurde der Gruppe I der zirkulären Bacteriocine zugeordnet, dessen Mitglieder sich durch schwache Homologie untereinander, aber durch wahrscheinlich ähnliche 3D-Strukturen auszeichnen. Die Masse beträgt 6381 Da und die Substanz ist stark aktiv gegen grampositive Bakterien. Das Biosynthesecluster umfasst sechs Gene für die Synthese, den Export, die Zyklisierung und die Immunität. Plantazolicin ist ein hydrophobes, stark modifiziertes Peptid aus der TOMM-Gruppe, einer Gruppe aus Microcin B17-ähnlichen Peptiden, die nach neueren Erkenntnissen verbreiteter ist, als bisher bekannt. Plantazolicin ist schwach aktiv gegen grampositive Bakterien und besitzt die Masse 1335 Da. Das Biosynthesecluster umfasst zwölf Gene, mit allen nötigen Genen für Synthese, Modifikation, Regulation, Immunität und Export.
Bacillus amyloliquefaciens FZB42 is a Gram-positive, plant-associated bacterium, which stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. Five gene clusters direct the non-ribosomal synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, an unknown peptide and the iron-siderophore bacillibactin. Three gene clusters direct the non-ribosomal synthesis of the antibacterial acting polyketides macrolactin, bacillaene and difficidin; in addition to the non-ribosomal synthesis of the antibacterial dipeptide bacilysin. Genes involved in ribosome-dependent synthesis of lantibiotics and other peptides are scarce. Only two incomplete gene clusters directing immunity against mersacidin and subtilin were found. In this work two ribosomally synthesized antibacterial peptides, amylocyclicin A and plantazolicin, and their corresponding gene clusters were identified. Amylocyclicin A is a circular peptide with a mass of 6381 Da and strong activity against Gram-positive bacteria. Six genes are responsible for the synthesis, maturation, export and immunity of this peptide belonging to group I of circular bacteriocins. Plantazolicin is a strongly modified hydrophobic peptide bearing a molecular mass of 1,335 Da and displaying antibacterial activity toward closely related Gram-positive bacteria. Essential modification contains the incorporation of azole heterocycles, which derive from Cys, Ser, and Thr residues of the precursor peptide and addition of two methyl groups. Twelve genes are responsible for synthesis, modification, export and immunity of this peptide belonging to the TOMM group of thiazol/oxazol modified microcins.
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Benitez, Lisianne Brittes. "Caracterização de peptídeos antimicrobianos de Bacillus amyloliquefaciens com atividade antibacteriana, antifúngica e amebicida." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2010. http://hdl.handle.net/10183/29950.

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Bacteriocinas são peptídeos pequenos sintetizados por bactérias que possuem propriedades antimicrobianas. O presente estudo teve por objetivos identificar e caracterizar uma bacteriocina produzida por Bacillus amyloliquefaciens LBM 5006, isolado de solo da Mata Atlântica, Santa Catarina, Brasil. A produção da substância antimicrobiana tem início na fase exponencial de crescimento e sua atividade máxima ocorre na fase estacionária. O efeito do cocultivo de B. amyloliquefaciens com diferentes bactérias na produção do antimicrobiano foi investigado. O cultivo de células de Escherichia coli intactas ou inativadas pelo calor com a cepa produtora aumentou a síntese da substância antimicrobiana. A bacteriocina LBM 5006 apresentou amplo espectro de ação, produzindo atividade antagonista contra bactérias, fungos e amebas patogênicas. O modo de ação contra células de Listeria monocytogenes e Paenibacillus larvae foi bactericida e bacteriolítico. Atividade esporicida foi observada contra esporos de P. larvae após tratamento com 1600 UA mL-1. Efeitos amebistático e amebicida contra trofozoítos de Acanthamoeba polyphaga com a consequente lise celular foram observados. A bacteriocina não teve efeito inibitório em células Vero nas concentrações que foram efetivas contra as amebas. A substância antimicrobiana foi isolada por precipitação com sulfato de amônio, cromatografia de gel filtração e extração com 1-butanol. O espectro ultravioleta foi típico de um polipeptídeo e o infravermelho indicou a presença de ligações peptídicas e grupamentos acil na sua estrutura. Análises por espectroscopia de massas indicaram que B. amyloquefaciens LBM 5006 produziu dois peptídeos antimicrobianos, com 1058 Da e 1464 Da, correspondentes a peptídeos tipo-iturina e tipo-fengicina, respectivamente.
Bacteriocins are small peptides synthesized by bacteria which have antimicrobiall properties. This study aimed to identify and characterize a bacteriocin produced by Bacillus amyloliquefaciens LBM 5006, isolated from soil of Mata Atlântica, Santa Catarina, Brazil. It has been observed that the antimicrobial substance production starting at the exponencial grown phase and maximum activity occur at stationary phase. The effect of different bacteria on the production of antimicrobial activity by Bacillus amyloliquefaciens LBM 5006 was investigate. It was concluded that the presence of intact or thermally inactivated cells of Escherichia coli enhanced the synthesis of antimicrobial peptides by B. amyloliquefaciens strain. Bacteriocin LBM 5006 showed broad spectrum of action, producing antagonistic activity against bacteria, fungi and pathogenic amoebas. The mode of action against Listeria monocytogenes and Paenibacillus larvae was bactericidal and bacteriolytic. Sporicidal activity was observed against P. larvae spores after treatment whit 1600 AU mL-1. Amoebicidal and amoebistatic effects were detected against throphozoites of Acanthamoeba poliphaga and cell lysis. The bacteriocin had no inhibitory effect on Vero cells at concentrations that were effective against amoebas.The antimicrobial substance was isolated by ammonium sulfate precipitation, gel filtration chromatography and 1-butanol extraction.The ultraviolet spectrum was typical of a polypeptide and the infrared spectrum indicates the presence of peptide bonds and acyl group(s) in its structure. Mass spectroscopy analysis indicated that B. amyloliquefaciens LBM 5006 produces two antimicrobial peptides, with main peaks at m/z 1058 Da and 1464 Da, corresponding to iturinlike and fengycin-like peptides, respectively.
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Larini, Mariana Munhoz. "Produção de surfactina por Bacillus amyloliquefaciens MO.04b em mistura de resíduos agroindustriais." Universidade Estadual de Londrina. Centro de Ciências Exatas. Programa de Pós-Graduação em Biotecnologia, 2017. http://www.bibliotecadigital.uel.br/document/?code=vtls000216058.

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O Brasil, importante no cenário agrícola mundial, é produtor de soja, grão de alto teor proteico, cana de açúcar e arroz. Essas culturas, quando processadas, geram materiais que são potenciais substratos para o cultivo microbiano e produção de metabólitos de alto valor agregado. A fermentação em estado sólido (FES) possibilita o cultivo de microrganismos em condição de baixo teor de umidade e favorece o aproveitamento de diferentes resíduos agroindustriais. O objetivo deste trabalho foi avaliar a produção de surfactina, um biossurfctante lipopeptídico, por FES, utilizando o Bacillus amyloliquefaciens MO.04b. O microrganismo foi cultivado em mistura de resíduos agroindustriais contendo farelo de soja, bagaço de cana-de-açúcar e casca de arroz, umedecidos com solução de sais acrescida de glicose 0,2 % (m/v), inoculados com suspensão de células (107 - 108 UFC mL-1) e incubados a 37 ± 2 °C. Após a interrupção da FES com água destilada, a mistura foi homogeneizada, a biomassa determinada por turbidimetria a 600 nm e contagem das unidades formadoras de colônias (UFC). Essa mistura foi submetida a centrifugação a 3.500 x g por 15 minutos. O peso do material sólido fermentado foi determinado por gravimentria e o extrato livre de células (ELC) utilizado para a determinação do pH (inicial e final), da atividade das enzimas proteases, xilanases, celulases e lacases, das proteínas totais e dos açúcares totais e redutores. Os lipipeptídeos contidos no ELC foram precipitados com adição de HCl 6 mol L-1 até pH 2,0 e mantido em repouso durante 12 horas e extraídos com a mistura de clorofórmio e metanol [CHCl3:CH3OH (4:1)]. O material obtido foi então liofifizado. A quantificação de surfactina foi realizada por cromatografia líquida de alta eficiência (CLAE). O pH final dos cultivos foi crescente ao longo do tempo de cultivo e atingiu o máximo de 8,5 em 72 horas, assim como a biomassa, que cresceu ao longo do tempo, até 18 – 24 horas, atingindo o máximo de 1 x 108 UFC mL-1. Quanto ao material sólido fermentado, foi verificada a redução progressiva do peso seco, de 1,2207g (0 horas) até 0,9268 g (72 horas), mostrando que em 72 horas de cultivo o microrganismo hidrolisou 25 % do total dos resíduos agroindustriais. A atividade de proteases e xilanases foram 0,946 U mL-1 e 0,81 U mL-1, respectivamente, entre 6 e 12 horas de cultivo. Não foram detectadas atividades de celulase e lacase. A surfactina foi produzida em todos os períodos analisados, e teve maior produção em 18 horas de cultivo, de 64,15 mg mL-1. A mistura de resíduos utilizada favoreceu o crescimento do microrganismo, o qual foi capaz de consumir 25 % dos resíduos nas condições de FES apresentadas. Tais condições propiciaram à bactéria a produção das enzimas protease e xilanase e de surfactina, cuja maior produção ocorreu em 18 horas de cultivo.
Brazil, important in the world's agricultural scenario, is a producer of soybeans, high-protein grain, sugar cane and rice. These cultures, when processed, generate materials that are potential substrates for the microbial culture and production of metabolites of high added value. A solid state fermentation (FES) allows the cultivation of microorganisms in a condition of low moisture content and favors the use of different agroindustrial residues. The objective of this work is to evaluate the production of surfactin, a lipopeptide biosurfactant, by FES using Bacillus amyloliquefaciens MO.04b. The microorganism was cultivated in a mixture of agroindustrial residues containing soybean meal, sugarcane bagasse and rice husk, moistened with salt solution plus 0.2% glucose (m/v), inoculated with cell suspension (107-108 CFU ml-1) and incubated at 37 ± 2 °C. After interruption of FES with distilled water, the mixture was homogenized, the biomass determined by turbidimetry at 600 nm and counting of the colony forming units (CFU). This mixture was subjected to centrifugation at 3500 x g for 15 min. The weight of the fermented solid material was determined by gravimetry and the free cell extract (ELC) used for the determination of the pH (initial and final), activity of enzymes protease, xylanases, cellulases and laccases, total proteins, total sugars and reduced sugar. The lipids contained in the ELC were precipitated with addition of 6 mol L-1 HCl to pH 2.0 and held for 12 hours and extracted with a mixture of chloroform and methanol [CHCl3:CH3OH (4:1)]. The obtained material was then lyophilized. The quantification of surfactin was performed by high performance liquid chromatography (HPLC). The final pH of the cultures was increased over the growing time and reached the maximum of 8.5 in 72 hours, as well as the biomass, which grew over time, up to 18 - 24 hours, reaching a maximum of 1 x 108 CFU mL-1. As regards the fermented solid material, a progressive reduction of dry weight was observed from 1.2207g (0 h) to 0.9268 g (72 h), showing that in 72 h of culture the microorganism hydrolyzed 25% of the total agroindustrial waste. The activity of proteases and xylanases were 0.946 U mL-1 and 0.81 U mL-1, respectively, between 6 and 12 hours of culture. No cellulase and laccase activities were detected. Surfactin was produced in all analyzed periods, and had a higher production in 18 hours of culture, of 64.15 mg mL-1. The mixture of residues used favored the growth of the microorganism, which was able to consume 25% of the residues under the FES conditions presented. These conditions allowed the bacterium to produce protease and xylanase and surfactin enzymes, whose highest production occurred in 18 hours of culture.
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Books on the topic "Amyloliquefaciens"

1

Hewitt, Christopher Julian. The synthesis of alpha-amylase (E.C.3.2.1.1.) by Bacillus amyloliquefaciens B20. Birmingham: University of Birmingham, 1993.

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Hillier, Peter K. The effect of nutrient limitation on the cell growth and alpha-amylase(E.C.3.2.1.1): Production of Bacillus amyloliquefaciens B155 in continuous culture. Birmingham: University of Birmingham, 1996.

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Book chapters on the topic "Amyloliquefaciens"

1

Bawden, M. J., T. Litjens, T. R. Hercus, B. K. May, and W. H. Elliott. "Extracellular Protease Production by B. Amyloliquefaciens." In Extracellular Enzymes of Microorganisms, 89–92. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4684-1274-1_11.

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Vehmaanperä, Jari. "Bacillus amyloliquefaciens — Production Host for Industrial Enzymes." In Electrotransformation of Bacteria, 119–23. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-662-04305-9_14.

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Wang, Depei, Kele Li, Yingying Wang, Ying Yang, and Jian Zhang. "Purification and Characterization of Antifungal Lipopeptide from Bacillus amyloliquefaciens BI2." In Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), 465–75. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37916-1_48.

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Hamdache, Ahlem, Mohammed Ezziyyani, and Ahmed Lamarti. "Evaluation of Strawberry Seed Treatments with Biological Control Agents Bacillus amyloliquefaciens." In Advances in Intelligent Systems and Computing, 175–82. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-36664-3_20.

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Borriss, Rainer. "Phytostimulation and Biocontrol by the Plant-Associated Bacillus amyloliquefaciens FZB42: An Update." In Bacilli and Agrobiotechnology, 163–84. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-44409-3_8.

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Asraoui, Meryem, Filipo Zanella, Stefania Marcato, Andrea Squartini, Jamila Amzil, Ahlem Hamdache, Barbara Baldan, and Mohammed Ezziyyani. "Bacillus amyloliquefaciens Enhanced Strawberry Plants Defense Responses, upon Challenge with Botrytis cinerea." In Advances in Intelligent Systems and Computing, 46–53. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-11878-5_5.

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Wang, Yajun, Zhanglei Cao, Miao Yu, and Depei Wang. "Application of Orthogonal Design to Optimize Fermentation Conditions of Bacillus amyloliquefaciens BI2." In Lecture Notes in Electrical Engineering, 487–96. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-46318-5_51.

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Song, Ping, Guang-lun Lei, Xin Ma, Pei-jun Cai, and Cheng-cheng Chu. "Basic Research on the Application of Enzyme Breaker Produced by Bacillus Amyloliquefaciens." In Springer Series in Geomechanics and Geoengineering, 2857–63. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-2485-1_263.

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Borriss, Rainer. "Phytostimulation and Biocontrol by the Plant-Associated Bacillus amyloliquefaciens FZB42: An Update." In Environmental and Microbial Biotechnology, 1–20. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-2576-6_1.

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Borriss, Rainer. "Comparative Analysis of the Complete Genome Sequence of the Plant Growth-Promoting Bacterium Bacillus amyloliquefaciens FZB42." In Molecular Microbial Ecology of the Rhizosphere, 883–98. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118297674.ch83.

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Conference papers on the topic "Amyloliquefaciens"

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Tarakanov, R. I., P. A. Vasilyev, K. S. Troshin, and F. S. U. Dzhalilov. "Activity of Bacillus amyloliquefaciens MBI600 against soybean bacterioses." In Agrobiotechnology-2021. Publishing house of RGAU - MSHA, 2021. http://dx.doi.org/10.26897/978-5-9675-1855-3-2021-187.

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the article presents data on the effectiveness of biological preparations based on bacteria of the genus Bacillus against pathogens of soybean bacteriosis. The results show high activity of the biofungicide based on Bacillus amyloliquefaciens MBI600 both in vitro and in the vegetative experiment against bacterial blight.
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Šarmaitytė, Luka, Aušra Zigmontienė, and Domantas Tracevičius. "MAISTO ATLIEKŲ AEROBINIO FERMENTAVIMO PANAUDOJANT BAKTERIJAS (BACILLUS SUBTILIS, LACTOBACILLUS SALIVARIUS, BACILLUS AMYLOLIQUEFACIENS) IR JUODOSIOS PLOKŠČIAMUSĖS (HERMETIA ILLUCENS) LERVAS TYRIMAS." In 22-oji jaunųjų mokslininkų konferencijos „Mokslas – Lietuvos ateitis“ teminė konferencija APLINKOS APSAUGOS INŽINERIJA. Vilnius Gediminas Technical University, 2020. http://dx.doi.org/10.3846/aainz.2019.014.

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Lietuvoje kasmet susidaro daugiau kaip 100 tūkst. tonų maisto atliekų, kurių didžioji dalis patenka į sąvartynus – labiausiai vengtinus atliekų tvarkymo įrenginius. ES šalyse pramonėje ir individualiuose namuose siekiama įdiegti žiedinės ekonomikos standartus atitinkančias kompostavimo technologijas. Viena iš jų – aerobinė fermentacija mikroorganizmais. Tyrimų tikslas – įvertinti trijų bakterijų rūšių įtaką juodosios plokščiamusės lervų prieaugiui aerobiškai fermentuojant augalinės kilmės maisto atliekas. Tyrimo objektai – Bacillus subtilis, Lactobacillus salivarius, Bacillus amyloliquefaciens bakterijos, Hermetia illucens lervos ir augalinės kilmės maisto atliekos. Eksperimentiniai tyrimai atlikti VGTU Aplinkos apsaugos ir vandens inžinerijos katedros laboratorijose naudojant termostatą, kai fermentacijos trukmė – 7 paros. Tyrimai parodė, kad Bacillus amyloliquefaciens yra tinkamiausia bakterija atlikti augalinės kilmės maisto atliekų aerobinę fermentaciją, nes išgyveno 96,0 vnt. lervų, kurių prieaugis siekė 2,422 g. Mėginiai su Lactobacillus salivarius bakterija parodė prasčiausius rezultatus: vidutiniškai išgyveno 95,7 vnt. lervų, kurių prieaugis buvo 1,599 g. Drėgmės tyrimas parodė, kad lervų išgyvenamumas yra geresnis mažesnės drėgmės terpės mėginiuose (59,29 % – 487 vnt.).
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Depei Wang, Hui Meng, Ting Zhou, and Kele Li. "Study on fermentation conditions of antibacterial substance from Bacillus amyloliquefaciens BI2." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5965323.

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Zhou, C., S. Xu, M. Zhang, Y. Chen, X. Guan, S. Wu, and Z. Bai. "Utilization of sweet potato starch wastewater for biofertilizer production byBacillus amyloliquefaciens." In SUSTAINABLE IRRIGATION 2012. Southampton, UK: WIT Press, 2012. http://dx.doi.org/10.2495/si120201.

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Xu, Yun-Long, Xiang-Rong Liu, and Yao-Wen Zhang. "The biosolubilization of Shenfu coal by Bacillus amyloliquefaciens and Saccharothrix xinjiangensis." In 2017 6th International Conference on Energy and Environmental Protection (ICEEP 2017). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/iceep-17.2017.83.

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Lu, Hedong, Chengyuan Gu, Panping Yang, Hai Xu, Muhanmmad Bilal, and Yan Ding. "Optimization of Cr(VI) Adsorption by Bacillus amyloliquefaciens and Its Mechanism Study." In The International Conference on Biomedical Engineering and Bioinformatics. SCITEPRESS - Science and Technology Publications, 2022. http://dx.doi.org/10.5220/0011382700003443.

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Lu, Hedong, Chengyuan Gu, Tao Yan, Qihan Zhang, Chengxin Geng, and Can Lv. "Screening and Identification of Bacillus amyloliquefaciens from the Gut of Aquatic Animals." In The International Conference on Biomedical Engineering and Bioinformatics. SCITEPRESS - Science and Technology Publications, 2022. http://dx.doi.org/10.5220/0011297900003443.

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Wu, Fei, Xixian Xie, Jianming Shi, Qingyang Xu, and Ning Chen. "Molecular Cloning, Expression and Enzymatic Characterization of Inosine Monophosphate Dehydrogenase from Bacillus amyloliquefaciens." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5518002.

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Zhao, Hailan, Yao Peng, Jing Xu, Haoming Xu, Wenqi Huang, Jiaqi Wang, Xue Guo, et al. "IDDF2021-ABS-0200 Bacillus amyloliquefaciens combined with resistant starch to ameliorate intestinal inflammation." In Abstracts of the International Digestive Disease Forum (IDDF), Hong Kong, 4–5 September 2021. BMJ Publishing Group Ltd and British Society of Gastroenterology, 2021. http://dx.doi.org/10.1136/gutjnl-2021-iddf.58.

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Abreu, Luciana De Paiva Santos, Carlos Eduardo De Souza Teodoro, and Ana Paula Martinazzo. "ATIVIDADE ANTIFÚNGICA IN VITRO DO SOBRENADANTE DA BACTÉRIA BACILLUS AMYLOLIQUEFACIENS SOBRE O FUNGO ASPERGILLUS FLAVUS." In II Congresso Brasileiro de Ciências Biológicas On-line. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1300.

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Introdução: O Aspergillus flavus é um fungo endêmico do solo, foi identificado como grande produtor de aflatoxina, a contaminação ocorre tanto durante o armazenamento, quanto ainda na fase de plantio. O milho é uma das culturas mais afetadas pela espécie, levando à sua contaminação por aflatoxina, e posteriormente esta é repassada aos animais que o consomem. O uso de fungicidas químicos é amplamente aplicado, no entanto esta prática pode levar à contaminação do grão. O biocontrole vêm como uma alternativa sustentável para substituir os defensivos agrícolas químicos. Objetivos: Na bactéria Bacillus amyloliquefaciens–VR002 (BA) foram identificadas características relacionadas ao seu papel como antagonista de microrganismos patogênicos. Analisou-se a atuação do sobrenadante da bactéria BA-VR002 no crescimento micelial do fungo Aspergillus flavus. Material e métodos: Os experimentos ocorreram no Laboratório de Biotecnologia/UFF-VR. A bactéria foi crescida utilizando 150mL do meio Luria Bertani esterilizado e inoculado com a cultura de BA-VR002 deixando-a crescer por 48h em estufa à 37ºC e 170rpm. Após o tempo de fermentação, o caldo foi centrifugado à 3500rpm, à 4ºC por 30min, filtrado em filtro de 22μm para um erlenmeyer estéril. Este filtrado foi então misturado ao meio ágar LB à 50±10ºC nas proporções de: 0%(v/v); 10%(v/v); 20%(v/v) e 30%(v/v). Este foi vertido em placas de Petri estéreis, após o endurecimento do ágar foram dispostos discos de micélio contendo o fungo A. flavus no centro das placas incubando-as em estufa à 30ºC por 5 dias. Os testes foram feitos em sextuplicata, e a área de crescimento foi calculada por meio do programa ImageJ. Resultados: Os resultados mostram que o percentual de inibição fúngico máximo ocorreu na concentração de 30% de sobrenadante, atingindo 96,4% de inibição do crescimento micelial quando comparado ao controle. Na concentração de 10% a inibição foi de 14,8%, na de 20% foi de 84,6%, confirmando a presença das substancias antifúngicas produzidas pela BA-VR002. Conclusão: O sobrenadante da bactéria B. amyloliquefaciens-VR002 contém substâncias antifúngicas, sendo a concentração de 30% a mais eficaz, mostrando-se como um potencial agente de controle do fungo A. flavus.
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