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Journal articles on the topic "ALG1"

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Ji, Shi-Qi, Bing Wang, Ming Lu, and Fu-Li Li. "Defluviitalea phaphyphila sp. nov., a Novel Thermophilic Bacterium That Degrades Brown Algae." Applied and Environmental Microbiology 82, no. 3 (November 20, 2015): 868–77. http://dx.doi.org/10.1128/aem.03297-15.

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ABSTRACTBrown algae are one of the largest groups of oceanic primary producers for CO2removal and carbon sinks for coastal regions. However, the mechanism for brown alga assimilation remains largely unknown in thermophilic microorganisms. In this work, a thermophilic alginolytic community was enriched from coastal sediment, from which an obligate anaerobic and thermophilic bacterial strain, designated Alg1, was isolated. Alg1 shared a 16S rRNA gene identity of 94.6% withDefluviitalea saccharophilaLIND6LT2T. Phenotypic, chemotaxonomic, and phylogenetic studies suggested strain Alg1 represented a novel species of the genusDefluviitalea, for which the nameDefluviitalea phaphyphilasp. nov. is proposed. Alg1 exhibited an intriguing ability to convert carbohydrates of brown algae, including alginate, laminarin, and mannitol, to ethanol and acetic acid. Three gene clusters participating in this process were predicted to be in the genome, and candidate enzymes were successfully expressed, purified, and characterized. Six alginate lyases were demonstrated to synergistically deconstruct alginate into unsaturated monosaccharide, followed by one uronic acid reductase and two 2-keto-3-deoxy-d-gluconate (KDG) kinases to produce pyruvate. A nonclassical mannitol 1-phosphate dehydrogenase, catalyzingd-mannitol 1-phosphate to fructose 1-phosphate in the presence of NAD+, and one laminarase also were disclosed. This work revealed that a thermophilic brown alga-decomposing system containing numerous novel thermophilic alginate lyases and a unique mannitol 1-phosphate dehydrogenase was adopted by the natural ethanologenic strain Alg1 during the process of evolution in hostile habitats.
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Truong, Vinh. "Effects of type and concentration of alginate on microencapsulation characteristics of lime essential oil (Citrus aurantifolia) produced by extrusion-dripping methods." Journal of Agriculture and Development 19, no. 01 (February 28, 2020): 65–76. http://dx.doi.org/10.52997/jad.9.01.2020.

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The extrusion-dripping method to produce alginate-calcium beads for microencapsulation of lime oil (Citrus aurantifolia) was carried out in this study. The experimental range of alginate concentration was from 1 to 4%. Above 1% alginate concentration, viscosity was pseudoplastic behavior. The size (1.52 - 1.57 mm) and sphericity (above 95%) of the beads were maximum at alginate concentration of 2 - 3%. The extrusion-dripping method was not applicable when alginate concentration was over 3.5% due to the high viscosity resulting in low sphericity. The two types of alginates with a protein content of 9% (alg1) and 2% (alg2) had the same microencapsulation yield of 73 - 74%. However, the solid recovery of alg2 (98.99%) was much higher than that of alg1 (52.71%). This is because alg2 has a higher purity and if it is used in production, it is easier to control the content of active ingredients and reduce the amount of organic waste that is harmful to the environment compared to alg1.
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Gao, X. D. "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum." Glycobiology 14, no. 6 (January 22, 2004): 559–70. http://dx.doi.org/10.1093/glycob/cwh072.

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Ramírez, Ana S., Jérémy Boilevin, Chia-Wei Lin, Bee Ha Gan, Daniel Janser, Markus Aebi, Tamis Darbre, Jean-Louis Reymond, and Kaspar P. Locher. "Chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins." Glycobiology 27, no. 8 (June 1, 2017): 726–33. http://dx.doi.org/10.1093/glycob/cwx045.

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JOUSSE-JOULIN, SANDRINE, MARIA ANTONIETTA d’AGOSTINO, THIERRY MARHADOUR, JEAN DAVID ALBERT, JACQUES BENTIN, ISABELLE CHARY VALCKENAERE, FABIEN ETCHEPARE, et al. "Reproducibility of Joint Swelling Assessment by Sonography in Patients with Long-lasting Rheumatoid Arthritis (SEA-Repro Study Part II)." Journal of Rheumatology 37, no. 5 (March 15, 2010): 938–45. http://dx.doi.org/10.3899/jrheum.090881.

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Objective.To evaluate the intraobserver and interobserver reproducibility of B-mode and power Doppler (PD) sonography in patients with active long-standing rheumatoid arthritis (RA) comparatively with clinical data.Methods.In each of 7 patients being considered for a change in their RA treatment regimen, 7 healthcare professionals examined the 28 joints used in the Disease Activity Score 28-joint count (DAS28). Then 7 sonographers examined each of the 7 patients twice, using previously published B-mode and PD grading systems. The clinical reference standard was presence of synovitis according to at least 4/7 examiners. The sonographic reference standard was at least grade 1 (ALG1) or 2 (ALG2) synovitis according to at least 4/7 sonographers. Interobserver reproducibility of sonography was assessed versus the sonographer having the best intraobserver reproducibility. Agreement was measured by Cohen’s kappa statistic.Results.Intraobserver and interobserver reproducibility of B-mode and PD used separately was fair to good. Agreement between clinicians and sonographers at all sites using B-mode, PD, and both was 0.46, 0.37, and 0.36, respectively, for grade 1 synovitis; and 0.58, 0.19, and 0.19 for grade 2 synovitis. The number of joints with synovitis was smaller by physical examination (36.7%) than by B-mode with ALG1 (58.6%; p < 0.001). The number of joints with synovitis was higher by physical examination than by PD with both ALG1 (17.8%; p < 0.0001) and ALG2 (6.6%; p < 0.0001).Conclusion.PD findings explain most of the difference between clinical and sonographic joint assessments for synovitis in patients with long-standing RA.
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Zhang, Wenyue, Philip M. James, Bobby G. Ng, Xueli Li, Baoyun Xia, Jiang Rong, Ghazia Asif, et al. "A Novel N-Tetrasaccharide in Patients with Congenital Disorders of Glycosylation, Including Asparagine-Linked Glycosylation Protein 1, Phosphomannomutase 2, and Mannose Phosphate Isomerase Deficiencies." Clinical Chemistry 62, no. 1 (January 1, 2016): 208–17. http://dx.doi.org/10.1373/clinchem.2015.243279.

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Abstract BACKGROUND Primary deficiencies in mannosylation of N-glycans are seen in a majority of patients with congenital disorders of glycosylation (CDG). We report the discovery of a series of novel N-glycans in sera, plasma, and cultured skin fibroblasts from patients with CDG having deficient mannosylation. METHOD We used LC-MS/MS and MALDI-TOF-MS analysis to identify and quantify a novel N-linked tetrasaccharide linked to the protein core, an N-tetrasaccharide (Neu5Acα2,6Galβ1,4-GlcNAcβ1,4GlcNAc) in plasma, serum glycoproteins, and a fibroblast lysate from patients with CDG caused by ALG1 [ALG1 (asparagine-linked glycosylation protein 1), chitobiosyldiphosphodolichol β-mannosyltransferase], PMM2 (phosphomannomutase 2), and MPI (mannose phosphate isomerase). RESULTS Glycoproteins in sera, plasma, or cell lysate from ALG1-CDG, PMM2-CDG, and MPI-CDG patients had substantially more N-tetrasaccharide than unaffected controls. We observed a &gt;80% decline in relative concentrations of the N-tetrasaccharide in MPI-CDG plasma after mannose therapy in 1 patient and in ALG1-CDG fibroblasts in vitro supplemented with mannose. CONCLUSIONS This novel N-tetrasaccharide could serve as a diagnostic marker of ALG1-, PMM2-, or MPI-CDG for screening of these 3 common CDG subtypes that comprise &gt;70% of CDG type I patients. Its quantification by LC-MS/MS may be useful for monitoring therapeutic efficacy of mannose. The discovery of these small N-glycans also indicates the presence of an alternative pathway in N-glycosylation not recognized previously, but its biological significance remains to be studied.
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Dhamija, Radhika, and Chelsea Chambers. "Clinical and Molecular Characterization of ALG1-CDG." Pediatric Neurology Briefs 30, no. 2 (April 5, 2016): 14. http://dx.doi.org/10.15844/pedneurbriefs-30-2-5.

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Jain, Sumita, and Dennis E. Ohman. "Deletion of algK in Mucoid Pseudomonas aeruginosa Blocks Alginate Polymer Formation and Results in Uronic Acid Secretion." Journal of Bacteriology 180, no. 3 (February 1, 1998): 634–41. http://dx.doi.org/10.1128/jb.180.3.634-641.1998.

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ABSTRACT Chronic pulmonary infection with Pseudomonas aeruginosais a common and serious problem in patients with cystic fibrosis (CF). The P. aeruginosa isolates from these patients typically have a mucoid colony morphology due to overproduction of the exopolysaccharide alginate, which contributes to the persistence of the organisms in the CF lung. Most of the alginate biosynthetic genes are clustered in the algD operon, located at 34 min on the chromosome. Alginate biosynthesis begins with the formation of an activated monomer, GDP-mannuronate, which is known to occur via the products of the algA, algC, andalgD genes. Polymannuronate forms in the periplasm, but the gene products involved in mannuronate translocation across the inner membrane and its polymerization are not known. One locus of the operon which remained uncharacterized was a new gene called algKbetween alg44 and algE. We sequencedalgK from the mucoid CF isolate FRD1 and expressed it inEscherichia coli, which revealed a polypeptide of the predicted size (52 kDa). The sequence of AlgK showed an apparent signal peptide characteristic of a lipoprotein. AlgK-PhoA fusion proteins were constructed and shown to be active, indicating that AlgK has a periplasmic subcellular localization. To test the phenotype of an AlgK− mutant, the algK coding sequence was replaced with a nonpolar gentamicin resistance cassette to avoid polar effects on genes downstream of algK that are essential for polymer formation. The algKΔ mutant was nonmucoid, demonstrating that AlgK was required for alginate production. Also, AlgK− mutants demonstrated a small-colony phenotype on L agar, suggesting that the loss of AlgK also caused a growth defect. The mutant phenotypes were complemented by a plasmid expressingalgK in trans. When the algKΔ mutation was placed in an algJ::Tn501background, where algA was not expressed due to polar transposon effects, the growth defect was not observed. AlgK− mutants appeared to accumulate a toxic extracellular product, and we hypothesized that this could be an unpolymerized alginate precursor. High levels of low-molecular-weight uronic acid were produced by the AlgK− mutant. When AlgK−culture supernatants were subjected to dialysis, high levels of uronic acids diffused out of the dialysis sac, and no uronic acids were detectable after extensive dialysis. In contrast, the mucoid wild-type strain produced only polymerized uronic acids (i.e., alginate), whereas the algKΔ algJ::Tn501 mutant produced no uronic acids. Thus, the alginate pathway in an AlgK− mutant was blocked after transport but at a step before polymerization, suggesting that AlgK plays an important role in the polymerization of mannuronate to alginate.
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Albuquerque-Wendt, Andreia, Hermann J. Hütte, Falk F. R. Buettner, Françoise H. Routier, and Hans Bakker. "Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily." International Journal of Molecular Sciences 20, no. 19 (September 29, 2019): 4842. http://dx.doi.org/10.3390/ijms20194842.

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Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily. In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins. We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures. A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s. Most of these residues were shown to be essential for DPY19 function and are positioned in luminal loops that showed high conservation within the DPY19 family. Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily. Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices. In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm. We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.
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Jain, Sumita, and Dennis E. Ohman. "Role of an Alginate Lyase for Alginate Transport in Mucoid Pseudomonas aeruginosa." Infection and Immunity 73, no. 10 (October 2005): 6429–36. http://dx.doi.org/10.1128/iai.73.10.6429-6436.2005.

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ABSTRACT The opportunistic pathogen Pseudomonas aeruginosa secretes a capsule-like polysaccharide called alginate that is important for evasion of host defenses, especially during chronic pulmonary disease of patients with cystic fibrosis (CF). Most proteins for alginate biosynthesis are encoded by the 12-gene algD operon. Interestingly, this operon also encodes AlgL, a lyase that degrades alginate. Mutants lacking AlgG, AlgK, or AlgX, also encoded by the operon, synthesize alginate polymers that are digested by the coregulated protein AlgL. We examined the phenotype of an ΔalgL mutation in the highly mucoid CF isolate FRD1. Generating a true ΔalgL mutant was possible only when the algD operon was under the control of a LacIq-repressed trc promoter. Upon induction of alginate production with isopropyl-β-d-thiogalactopyranoside, the ΔalgL mutant cells were lysed within a few hours. Electron micrographs of the ΔalgL mutant showed that alginate polymers accumulated in the periplasm, which ultimately burst the bacterial cell wall. The requirement of AlgL in an alginate-overproducing strain led to a new model for alginate secretion in which a multiprotein secretion complex (or scaffold, that includes AlgG, AlgK, AlgX, and AlgL) guides new polymers through the periplasm for secretion across the outer membrane. In this model, AlgL is bifunctional with a structural role in the scaffold and a role in degrading free alginate polymers in the periplasm.
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Dissertations / Theses on the topic "ALG1"

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Revers, Leigh. "Studies on the expression, purification, and synthetic utility of recombinant yeast #beta#-1,4-mannosyltransferase." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318640.

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Stacey, Sean. "Virulence Regulation in Pseudomonas aeruginosa via the Alginate Regulators, AlgU and AlgR, the posttranscriptional regulator, RsmA, and the Two-component System, AlgZ/R." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etd/3486.

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Pseudomonas aeruginosa is a Gram-negative bacillus able to colonize a wide variety of environments. In the human host, P. aeruginosa can establish an acute infection or persist and create a chronic infection. P. aeruginosa is able to establish a niche and persist in human hosts by using a wide array of virulence factors used for: movement, killing host cells, and evading immune cells and antibiotics. Understanding virulence factors and their regulation has proved to be an important means of combating the morbidity and mortality of P. aeruginosa as well as the ever-increasing threat of drug resistance. By targeting virulence factors or their regulators with antivirulence compounds, the bacterium is rendered defenseless and more readily cleared by the immune system. In this study, we examine three different contributors to virulence factor regulation. First, we examined the role of the extracellular sigma factor AlgU and its contribution to regulating a post-transcriptional RsmA. AlgU is most commonly active in chronic infecting strains that produce copious amounts of the virulence factor, alginate. We confirmed that not only was their more RsmA in this background, but that there was a previously unidentified promoter for rsmA regulated by AlgU. In concert with this study, we followed up by studying the effects of AlgR on this unknown promoter. AlgU and AlgR are known to work together, specifically on the alginate operon, and we hypothesized based off of bioinformatics data this was the case with RsmA. Second, due to increased RsmA in this chronic infection strain, we set out to identify potential unknown virulence targets of RsmA. A previously unrevealed target, pasP, was shown to directly interact with RsmA. Third, in an acute infection model strain we identified a new regulatory loop involving the two-component system AlgZ/R. In a pilW strain deficient in the motility virulence factor type IV pili, we showed increased levels of AlgZ/R compared to wildtype, PAO1. The pilW strain produced less pyocyanin, rhamnolipid, and elastase and was attenuated in J774a.1 macrophages. Overall, these studies push the understanding of virulence factor regulation and open the door to potential therapeutic targets in treating P. aeruginosa infections.
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Kim, Jean. "Alternative regulation of the alginate algD operon by an activated AlgB in nonmucoid Pseudomonas aeruginosa is dependent on Sigma 54." VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/108.

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Alginate overproduction by Pseudomonas aeruginosa, which causes a mucoid phenotype, is a major virulence factor associated with chronic pulmonary infections in cystic fibrosis patients. Expression of the algD operon for alginate biosynthesis requires three major regulators in association with the ECF sigma factor, σ22, in mucoid strains that are typically defective in anti-sigma factor, MucA. One such algD regulator is AlgB, a member of the NtrC family of two-component systems, which typically utilize σ54. However, neither σ54 nor the cognate sensor kinase (KinB) of AlgB are required for algD expression in such mucoid strains. I hypothesized that KinB-phosphorylated AlgB must play some role in gene regulation, and so I sought to construct a constitutively active AlgB that simulated kinase-phosphorylation. I took a predictive approach and genetically introduced substitutions in AlgB that had been shown to activate DctD, a close homologue of AlgB in Rhizobium (52). When one such substitution, AlgBE125K, was transferred to a nonmucoid P. aeruginosa PAO ΔalgB-kinB (JK159) strain, alginate overproduction was observed. Interestingly, introduction of an algT mutation to remove σ22 did not block alginate production induced by AlgBE125K; although, it did stimulate the production of alginate in the presence of AlgBwt in trans to similar levels induced by the constitutive mutant. In contrast, introduction of an rpoN mutation showed that alginate production mediated by AlgBwt and AlgBE125K was σ54 dependent. The increase in expression of alginate by AlgBwt in the presence of σ54 and the absence of σ22 suggested a competition between the sigma factors for binding to PalgD. Biochemical assays were conducted to assess the constitutive property of AlgBE125K. For the ATPase assay, an equivalent amount of ATP hydrolysis was observed between the mutant and the wild type AlgB proteins. Slight differences seen for the EMSA data suggested possible higher order complex formation for AlgBE125K compared to AlgBwt. Collectively, these results suggested that in wild-type (MucA+) P. aeruginosa, expression of the algD operon is dependent on the phosphorylation of AlgB by KinB in a typical two-component fashion that is triggered by some as yet unknown environmental stimulus.
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Williams, Danielle A. "The AlgZ/R Two-Component System Is Responsible for Attenuation of Virulence in Pseudomonas aeruginosa." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etd/3340.

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Pseudomonas aeruginosa is an important opportunistic pathogen. Many P. aeruginosa virulence factors are regulated by the AlgZ/R two component system. AlgZ is the sensor histidine kinase which phosphorylates AlgR, the response regulator. AlgR activates transcription of different gene targets based upon its phosphorylation state. The genes that encode AlgZ and AlgR are transcribed in an operon. While regulation of algR expression has been well studied, regulation of algZ expression has not. Using a pilW mutant in concert with algZTF-lacZ transcriptional fusion, we conducted a transposon mutagenesis to identify algZ regulators. We identified an unknown autoregulatory loop. The type IV pilus minor pilins prevent the phosphorylation of AlgR by AlgZ . This inhibition of the AlgZ/R system subsequently down-regulates both the expression of the fimU operon and the algZ/R operon. Because AlgR regulates virulence, it is possible that virulence can also be reduced by targeting activation of the AlgZ/R system.
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Bickel, Tanja. "Biosynthese von Dolicholpyrophosphat-Chitobiose in Saccharomyces cerevisiae : Identifizierung und biochemische Charakterisierung von Alg13 und Alg14 als Untereinheiten eines neuartigen, dimeren Glykosyltransferase-Komplexes." kostenfrei, 2007. http://www.opus-bayern.de/uni-regensburg/volltexte/2008/882/.

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López, Lucía. "Aprender algo nuevo." Canalé, 2012. http://repositorio.pucp.edu.pe/index/handle/123456789/113973.

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Robles, Daniel, Sophia Wong, Manuel Coral, and Sergio Malásquez. "Ley Universitaria: Cambiar todo para que algo cambie……. (al menos algo)." Universidad Peruana de Ciencias Aplicadas (UPC), 2015. http://hdl.handle.net/10757/344089.

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Trabajo ganador durante en el Curso Taller de Periodismo Literario 2014-2, Facultad de Comunicación y Periodismo, Universidad Peruana de Ciencias Aplicadas - UPC. Lima, Perú
Trabajo ganador durante en el Curso Taller de Periodismo Literario 2014-2, Facultad de Comunicación y Periodismo, Universidad Peruana de Ciencias Aplicadas - UPC. Lima, Perú
La nueva ley universitaria o ley Nº 30220 se promulgó el 8 de julio del 2014 para enfrentar un gran problema estructural del Perú: el bajo nivel educativo de las universidades públicas y privadas. A grandes problemas, grandes soluciones; pero también grandes críticas y polémicas. En este reportaje acronicado, se mencionará y analizará la situación que llevó a la necesidad de la promulgación de esta ley, la mala situación educativa actual (sí, porque por más afiches de excelencia que se repartan junto con los diarios, los ránkings no muestran a alguna universidad peruana dentro de la primera división de centros educativos superiores del mundo; más adelante se mencionará en detalle en qué puestos estamos), los principales puntos de la ley que alterarán el sistema educativo, nuestra opinión sobre la ley, así como las omisiones y trabajo futuro que se debe realizar para que esta reforma cambie no necesariamente todo, sino algo.
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Hughes, Abigail. "PA2771 Affects algZ expression and AlgZ/R Phenotypic Outputs in Pseudomonas aeruginosa." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etd/3462.

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Pseudomonas aeruginosa is a central nosocomial pathogen that can infect any tissue in the human body. A two-component system in P. aeruginosa that regulates many virulence factors is the AlgZ/R system. A previously unidentified regulator of algZ, that does not affect algR, has been identified via transposon mutagenesis, ‘PA2771’. The mechanism of regulation has not been previously studied, and novel evidence of PA2771 functioning as a diguanyalate cyclase was observed. When PA2771 is active, cyclic di-GMP levels are high, promoting the upregulation of the fimU operon and Type VI pili. In the PA2771 mutant, an upregulation in the expression of the flagellar genes and swarming phenotype was observed, and restored via complementation. PA2771's function in regulating algZ expression, is likely indirect and alters virulence gene regulation and phenotypic outputs in P. aeruginosa in the switch between twitching and swimming motility, and appears to be specific to PA2771.
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Horikawa, Karina, and Cesar Molina. "Algo Rapidin (Take Out Food)." Tesis, Universidad de Chile, 2018. http://repositorio.uchile.cl/handle/2250/168159.

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Tesis para el grado de Magíster Administración de Negocios
Horikawa, Karina, [Parte I], Molina, Cesar, [Parte II]
estrategia de Panamá de convertirse en un hub logístico para la región ha traído como consecuencia que se desarrolle la infraestructura necesaria para cumplir con este objetivo como la ampliación del Aeropuerto Internacional de Tocumen con la construcción de una Zona Franca y la construcción de Parques Industriales en todo el país. Según estadísticas obtenidas, se tiene más de 620 mil metros cuadrados disponibles para la renta en Parques Industriales, el cual representa el 49% de participación. Esta información es relevante en el sector económico. Sin embargo, estos lugares no ofrecen un lugar donde los trabajadores puedan comprar sus alimentos. Es por eso que nace Algo Rapidin “Take Out Food” en el Parque Industrial Las Américas, el cual se encuentra ubicado en el corregimiento de Pacora el cual se localiza en la zona este del país. Esta idea de negocio es la venta de alimentos listos para ser consumidos a través de un innovador sistema que los mantendrá en un rango de temperatura ideal para que estén siempre listos para ser consumidos. Los principales atributos que tendrán los almuerzos serán precio y cantidad, los cuales fueron los mejores calificados en las encuestas y estudios de campo realizados durante el proceso de investigación. El precio del producto final será de $4 para el menú Económico y $6 para el Ejecutivo. En cuanto al VAN se obtuvo $258.935 y una TIR de 39%, la tasa de descuento obtenida fue de 13.95% con lo cual en el horizonte del proyecto de 5 años, este demuestra que es rentable y muy atractivo, con la posibilidad de lograr escalabilidad en el mediano plazo en Parques Industriales aledaños y en el largo plazo la posibilidad de expandirse a otro tipo de industria.
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Gomes, Camila Queiroz. "Caracterização de Geitlerinema UFV-E01 (Cyanobacteria) e Stigeoclonium UFV- E02 (Chlorophyta) cultivadas em presença de arsênio." Universidade Federal de Viçosa, 2005. http://www.locus.ufv.br/handle/123456789/8844.

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As cianobactérias e algas são organismos encontrados, abundantemente, em ambientes aquáticos, onde atuam como produtores primários transferindo energia para os diferentes níveis tróficos. A ocorrência de substâncias tóxicas ou xenobióticas tem sido uma ameça constante nos ecossistemas aquáticos. Tais substâncias podem causar efeitos tóxicos sobre diferentes grupos de cianobactérias e algas afetando em nível celular, de população e comunidade, causando prejuízos a cadeia trófica, nestes ambientes. O arsênio (As) é um elemento tóxico freqüentemente associado com depósitos de ouro. Através da mineração, o As é trazido à superfície pelo processo de drenagem ácida sendo, posteriormente, lixiviado e contaminando os corpos d água. Estudos evidenciam que cianobactérias e algas podem acumular As intracelularmente, sendo capazes de biotransformar as formas tóxicas em formas não tóxicas. A utilização destes processos permite a utilização destes organismos como biorremediadores na descontaminação de ambientes aquáticos contaminados pelo As. Com o objetivo de caracterizar os gêneros Geitlerinema UFV-E01 e Stigeoclonium UFV- E02, cultivados na presença de As, foram conduzidos, em laboratório, estudos sobre o crescimento, a absorção e a toxicidade do As. Para a caracterização foram utilizados como parâmetros à alteração morfológica, a produção de biomassa, para ambos os gêneros. A detecção do gene smtA que codifica a proteína metalotioneína (técnica de PCR), foi feita apenas em Geitlerinema UFV-E01. Os resultados mostraram que a absorção de As, em Geitlerinema UFV-E01, foi maior em células cultivadas em meio BG-11 B, com baixa concentração de fosfato (6,5 μmol.mL -1 ). A absorção de As foi proporcional às concentrações crescentes do elemento no meio de cultivo, atingindo o pico máximo de absorção em 24 horas de exposição (665,25 μg As.g -1 ms para células cultivadas em meio BG-11 A e 1.290,32 μg de As.g -1 ms, para células cultivadas em meio BG-11 B), sendo observado uma queda em 48 horas de exposição (500,00 μg As.g -1 ms para células cultivadas em meio BG-11 A, com 17,5 μmol.mL -1 de fosfato, e 810,00 μg de As.g -1 ms para células cultivadas em meio BG-11 B). As células de Geitlerinema UFV-E01 não apresentaram alterações morfológicas e não houve redução na produção de biomassa. Entretanto, foi observada a presença de grânulos densos no citoplasma das células. Os resultados obtidos, por meio da técnica de PCR, mostraram que Geitlerinema UFV-E01 não possui o gene smtA que codifica a proteína metalotioneína, sugerindo que a cianobactéria não utiliza esta estratégia nos processos de detoxificação celular. A absorção de As pelas células de Stigeoclonium UFV-E02, não foram influenciadas pelas concentrações de fosfato no meio BG-11 líquido. A absorção máxima de As ocorreu em 24 horas de exposição (1.300 μg As.g - ms) e foi mantida constante em 48 horas de exposição. Entretanto, em Stigeoclonium UFV- E02, houve redução na produção de biomassa e, também, alterações morfológicas nos filamentos caracterizados pelo aparecimento de células estreitas e longas, com cloroplastos deformados e de tamanho reduzido quando comparado com células sadias (controle).
Cianobacteria and algae are important primary producers in aquatic ecosystems and their energy is transferred throughout the different levels of the environmental trophic chain. The presence of xenobiotic or toxic elements has been a constant threat to aquatic ecosystems. These elements can cause considerable negative effects on microorganisms, since it can affect their different organization levels and consequently impairing the whole trophic chain. The arsenic (As) is a potential toxic element frequently associated with gold deposits. It can be brought to the surface by the gold mining activity, alongside the acid drainage and latter lixiviated, causing water bodies contamination. Previous study has shown that cianobacteria and algae can accumulated toxic As within the cells and sometimes transforming it into a non toxic form. Such trait can be useful for bioremediation purpose, by using these organisms on As decontamination of aquatic environments. In order to assess the bioremediator potential of Geitlerinema UFV-E01 (Cyanobacteria) and Stigeoclonium UFV-02 genera, absorption and toxicity tests were carried out on selected lineages, cultivated in presence of As, under laboratory conditions. The morphological alterations and biomass production were used as evaluation parameters to portray the two genera in relation to As presence. The identification of the smtA gene, which codifies the metallotioneina, was performed on the Geitlerinema UFV-E01 genus by PCR technique. The results indicated that the As absorption by Geitlerinema UFV-E01 cells was greater when they were cultivated in low phosphorus content (6,5 μmomL -1 ) BG-11 medium. The As absorption was proportional to the increase on the element content in culture medium, reaching the maximum after 24 hours of exposure either in the BG-11A medium (665,25 μg As.g -1 ms in BG-11 A and 1.290,32 μg As.g -1 ms in medium BG-11 B). After 48 hours of exposure to As, it was observed a decrease in the amount of element in the cells on both mediums tested, (500,00 μg As.g -1 ms in medium BG- 11 A with 17,5 μmol.mL -1 of fosfato, and 810,00 μg de As.g -1 ms in medium BG-11 B).The G. cells did not shown any signs of morphological alteration or biomass production decay in any of the culture medium tested. Nevertheless, dense corpuscles were observed in the cytoplasm of the cells cultivated. The PCR results revealed that the Geitlerinema UFV-E01 genus does not have the SmtA gene, suggesting that this cianobacteria can be using other mechanism for As detoxification. As detoxification by S. cells was not influenced by the phosphate concentration in the BG-11 medium. The maximum As absorption took place after 24 hours of exposure (1,300μg As.g -1 ms) and stayed constant for 48 hours. It was observed that Stigeoclonium UFV-02 genus had a decrease in the production of biomass and morphological alterations. The filaments shown longer and striated cells, containing smaller and deformed chloroplasts when compared to the control material.
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Books on the topic "ALG1"

1

Hanŭl to algo ttang to algo. Kyŏnggi-do: Haenaem Chʻulpʻansa, 1987.

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1940-, Pak Kyŏng-nyong, ed. Sŏul ŭl algo yŏksa rŭl algo. Sŏul Tʻŭkpyŏlsi: Susŏwŏn, 2003.

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Alma alga. Lima, Perú: Borrador Editores, 2010.

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Cho, Sŏng-ho. Algo innŭnjiyo. [Pʻyŏngyang]: Kŭmsŏng Chʻŏngnyŏn Chʻulpʻansa, 1985.

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Algo erótico. México, D.F: Federación Editorial Mexicana, 1986.

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Serrat, Joan Manuel. Serrat: Algo personal. Madrid: Temas de Hoy, 2008.

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Ŭn-su, Kim, ed. Algi shwiun pŏbinsepŏp. 5th ed. Sŏul-si: Kyŏngyŏng kwa Hoegye, 2004.

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Niall, Binns, and Echeverria Ignacio, eds. Obras completas & algo +. Barcelona: Circulo de Lectores ; Galaxia Gutenberg, 2006.

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Thevenet, Homero Alsina. Algo sobre cine. Montevideo, Uruguay: Irrupciones Grupo Editor, 2013.

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Córdova, Anunciada Fernández de. De algo incierto. Madrid: SIAL Ediciones, 2004.

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Book chapters on the topic "ALG1"

1

Dean, Neta. "Alg1, Alg2, and Alg11 Mannosyltransferases of the Endoplasmic Reticulum." In Handbook of Glycosyltransferases and Related Genes, 1239–47. Tokyo: Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-54240-7_14.

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Dean, Neta, and Xiao-Dong Gao. "Heterodimeric Alg13/Alg14 UDP-GlcNAc Transferase (ALG13,14)." In Handbook of Glycosyltransferases and Related Genes, 1231–38. Tokyo: Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-54240-7_37.

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van Doorslaer, Luc. "Alternative labels for “translation”." In Handbook of Translation Studies, 4–10. Amsterdam: John Benjamins Publishing Company, 2021. http://dx.doi.org/10.1075/hts.5.alt1.

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Clarke, Robert. "¿Compraste algo?" In Mastering Spanish, 227–38. London: Macmillan Education UK, 1995. http://dx.doi.org/10.1007/978-1-349-13467-0_17.

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Helenius, Jonne H., and Claude A. Jakob. "ALG3 Mannosyltransferase." In Handbook of Glycosyltransferases and Related Genes, 557–64. Tokyo: Springer Japan, 2002. http://dx.doi.org/10.1007/978-4-431-67877-9_81.

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Schenk, Barbara, and Claude A. Jakob. "ALG6 Glucosyltransferase." In Handbook of Glycosyltransferases and Related Genes, 565–74. Tokyo: Springer Japan, 2002. http://dx.doi.org/10.1007/978-4-431-67877-9_82.

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Spittel, M., and T. Spittel. "AlMg1 (C)." In Part 2: Non-ferrous Alloys - Light Metals, 330–35. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-13864-5_53.

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Suski, W., and T. Palewski. "ALn1±xBi4±xS8." In Pnictides and Chalcogenides II, 1056–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/10713485_285.

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Hangay, George, Severiano F. Gayubo, Marjorie A. Hoy, Marta Goula, Allen Sanborn, Wendell L. Morrill, Gerd GÄde, et al. "Alga (pl., Algae)." In Encyclopedia of Entomology, 110. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_138.

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"Introduction to Solution-Focused Therapy." In Learning Solution-Focused Therapy. American Psychiatric Publishing, 2013. http://dx.doi.org/10.1176/appi.books.9781615370986.al01.

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Conference papers on the topic "ALG1"

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Katai, Zoltan. "ALGO-RYTHMICS." In the 2014 conference. New York, New York, USA: ACM Press, 2014. http://dx.doi.org/10.1145/2591708.2602684.

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Altaher, Marah, and Ahmed Ferchichi. "ALGO-THINK Approach." In 2018 JCCO Joint International Conference on ICT in Education and Training, International Conference on Computing in Arabic, and International Conference on Geocomputing (JCCO: TICET-ICCA-GECO). IEEE, 2018. http://dx.doi.org/10.1109/icca-ticet.2018.8726211.

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Janovits, Ricardo de Albuquerque, Thiago Camargo de Magalhães, Felipe Romero Lopes Neves, and Vinícius Barroso Hirota. "Musculação e emagrecimento: algo possível?" In Seminário Esportes 2018: Futebol e o Ano da Copa da Rússia. Revista Remecs, 2018. http://dx.doi.org/10.24281/rremecs.2018.04.11.se16-17.

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Bey, Anis, and Tahar Bensebaa. "ALGO+, an assessment tool for algorithmic competencies." In 2011 IEEE Global Engineering Education Conference (EDUCON). IEEE, 2011. http://dx.doi.org/10.1109/educon.2011.5773260.

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Cañas, María. "Investiga, activa y juega, que algo queda." In III Congreso Internacional de Investigación en Artes Visuales :: ANIAV 2017 :: GLOCAL. Valencia: Universitat Politècnica València, 2017. http://dx.doi.org/10.4995/aniav.2017.6897.

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Apuesta en torno al ámbito del vivir y del investigar que se centra en la experimentación artística a través de la “risastencia” (el humor de todos los colores y sabores), el juego, el Do It yourself (DIY), la agitación y las prácticas colaborativas de las multitudes conectadas, como estrategias de insurgencia o, si no, al menos, de resistencia y supervivencia popular. Desafío de defender la carcajada que organiza la rabia... o no, ¿por qué, y si esa rabia al final sólo queda datificada en los muros del ciberespacio? Defendamos a ultranza la no privatización y la liberalización de nuestra memoria histórica e imaginarios. Seamos activistas comprometidos con la cultura libre y con la idea de cultura como construcción colectiva, como contrahistoria, para practicar una cultura de oposición. ¡Vida eterna al dominio público! Apostemos por unas vidas dignas de ser vividas a lo María Zambrano, por el archivo orgánico de internet y el “detritus” audiovisual que nos rodea como herramientas de desarrollo cultural, y por la necesidad de educar e investigar en el hackeo y reciclaje de nuestros imaginarios, para así transformarnos en seres más libres, críticos y creativos. Seamos en las calles y con el Internet de las cosas o como la denomina O’Reilly, la web encontrando el mundo. Nos corresponde enseñar a personas conscientes y sensibles con lo que ocurre, adaptables a los cambios convulsos y que puedan responder de forma creativa, ecológica y ética a los problemas de nuestro tiempo.
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Aditya, Krishna, Chee-Hung H. Chu, and Harold H. Szu. "Fast algo-tectures for discrete wavelet transforms." In Aerospace/Defense Sensing and Controls, edited by Harold H. Szu. SPIE, 1996. http://dx.doi.org/10.1117/12.236037.

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S, Sweena Kripa, Lisa Margreat R, Shiny priscilla C, Sancy Swetha P, J. John Paul, and Thusnavis Bella Mary I. "Chatbot - Attendance and Location Guidance system (ALGs)." In 2021 3rd International Conference on Signal Processing and Communication (ICPSC). IEEE, 2021. http://dx.doi.org/10.1109/icspc51351.2021.9451793.

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Lian, Zongkai, Haiqiong Yang, Fan Wu, Mingxin Li, and Shancheng Jiang. "C-Algl Net: Pathological Images Generate Diagnostic Results." In 2020 IEEE 17th International Symposium on Biomedical Imaging Workshops (ISBI Workshops). IEEE, 2020. http://dx.doi.org/10.1109/isbiworkshops50223.2020.9153419.

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Guo, Xiujuan, Shurong Wang, Zuogang Guo, and Zhongyang Luo. "Properties of Bio-Oil from Alga Fast Pyrolysis." In 2011 Asia-Pacific Power and Energy Engineering Conference (APPEEC). IEEE, 2011. http://dx.doi.org/10.1109/appeec.2011.5748790.

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Khan, Muhammad Farhan, and Muhammad Imran Khan. "An extensive study on application level gateways (ALGs)." In 2011 IEEE 14th International Multitopic Conference (INMIC). IEEE, 2011. http://dx.doi.org/10.1109/inmic.2011.6151496.

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Reports on the topic "ALG1"

1

Lohrenz, Steven E., and Oscar M. Schofield. Optical Detection and Assessment of the Harmful Alga, Karenia brevis. Fort Belvoir, VA: Defense Technical Information Center, September 2003. http://dx.doi.org/10.21236/ada621233.

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López Rivas, Abelardo. Receptores de muerte celular: iniciadores de la apoptosis y algo más... Sociedad Española de Bioquímica y Biología Molecular (SEBBM), March 2012. http://dx.doi.org/10.18567/sebbmdiv_anc.2012.03.1.

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Owings, Raymond. Isolation and characterization of two sterols from the green alga, Selenastrum capricornutum. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.861.

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Goodenough, Ursula. Systems Biology of Lipid Body Formation in the Green Alga Chlamydomonas reinhardtii. Office of Scientific and Technical Information (OSTI), November 2017. http://dx.doi.org/10.2172/1408918.

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V. J. Fabry. Calcium Carbonate Production by Coccolithophorid Alge in Long Term Carbon Dioxide Sequestration. Office of Scientific and Technical Information (OSTI), September 2006. http://dx.doi.org/10.2172/895624.

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van, I. An FTP Application Layer Gateway (ALG) for IPv6-to-IPv4 Translation. RFC Editor, October 2011. http://dx.doi.org/10.17487/rfc6384.

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Worden, Alexandra Z., Stephen Callister, Joshua Stuart, and Richard Smith. Final Report: Connecting genomic capabilities to physiology and response: Systems biology of the widespread alga Micromonas. Office of Scientific and Technical Information (OSTI), September 2014. http://dx.doi.org/10.2172/1158817.

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Ades, Dennis. The role of iron nutrition in regulating patterns of photosynthesis and nitrogen metabolism in the green alga Scenedesmus quadricauda. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.5533.

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Villagra, N., B. López, and A. Monfort. La gestión de intangibles y la marca corporativa: ¿ha cambiado algo en la relación entre las empresas y la sociedad? Revista Latina de Comunicación Social, December 2015. http://dx.doi.org/10.4185/rlcs-2015-1072.

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Stead, A. D., T. W. Ford, A. M. Page, J. T. Brown, and W. Meyer-Ilse. X-ray dense cellular inclusions in the cells of the green alga Chlamydomonas reinhardtii as seen by soft-x-ray microscopy. Office of Scientific and Technical Information (OSTI), April 1997. http://dx.doi.org/10.2172/603459.

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