Dissertations / Theses on the topic 'Air - Pollution - Toxicology'
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Gorr, Matthew W. "Air Pollution and Cardiovascular Dysfunction." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1428674045.
Full textBillah, Md Baki. "Chemical and toxicological characterization of chemical contaminants in air pollution particulate matter." HKBU Institutional Repository, 2015. https://repository.hkbu.edu.hk/etd_oa/155.
Full textKim, Dasom. "PPAR-gamma Regulates T Cell Responses in Air Pollutant-associated Inflammation." University of Cincinnati / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1522414820700163.
Full textDemer, Frank Robert 1961. "The impact of various cure parameters on the release of 4-phenylcyclohexene from carboxylated styrene-butadiene rubber latex." Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/277075.
Full textBakand, Shahnaz Safety Science Faculty of Science UNSW. "Development of in vitro methods for toxicity assessment of workplace air contaminants." Awarded by:University of New South Wales. School of Safety Science, 2006. http://handle.unsw.edu.au/1959.4/24246.
Full textNorris, Mary Jo. "A Study of Radon in Air and Water in Maine Schools." Fogler Library, University of Maine, 2002. http://www.library.umaine.edu/theses/pdf/NorrisMJ2002.pdf.
Full textAlmeida, Daniela Sanches de. "Estudo da exposição pessoal ao material particulado atmosférico em Londrina." Universidade Tecnológica Federal do Paraná, 2016. http://repositorio.utfpr.edu.br/jspui/handle/1/1900.
Full textA exposição humana a poluentes atmosféricos causam efeitos adversos à saúde. As áreas urbanas com alta densidade populacional e fluxos de veículos elevados são regiões críticas. Os poluentes do ar são onipresentes, e certo nível de exposição é inevitável, variável e dependente de uma série de fatores ambientais e individuais de cada um. O presente trabalho visou medir e avaliar a exposição pessoal ao material particulado atmosférico por indivíduos da cidade de Londrina em um estudo com 30 voluntários de 6 classes de ocupação. Utilizando monitores pessoais de concentração de material particulado atmosférico foram medidas as concentrações em massa e número de partículas no ar inalado. Células bucais dos voluntários foram coletadas antes e após a exposição para a determinação da genotoxicidade pelo teste do cometa. Foram realizadas campanhas de amostragem no inverno, em rotas georreferenciadas que variaram de acordo com a rotina de cada indivíduo. A partir das concentrações medidas, tempo de exposição e massa corporal foram calculadas as doses recebidas individualmente. As concentrações em massa (média de 8 h) encontradas para os voluntários variaram de 2,41 a 34,07 µg m-3 para MP1,0 e de 23,11 a 11060,54 µg m-3 para o MP10. As concentrações em número de partículas encontradas variaram de 14 a 183 partículas cm-3 para o MP0,3; para o MP2,5 elas variaram de 0,20 a 27 partículas cm-3. A categoria Construção e Indústria apresentou as doses recebidas mais elevadas, enquanto o setor Administrativo apresentou as menores. Através do teste de Kruskal-Wallis foi verificado que há diferença significativa de danos no DNA nas classes Comércio, Construção e Indústria e Transporte, quando comparadas ao controle (classe Administrativo). O modelo que melhor se adequou aos dados foi o Binomial Negativo, indicando associação positiva, principalmente das classes de ocupação e número de partículas com os danos ao DNA das células bucais. Os resultados da simulação de deposição pulmonar a partir das concentrações obtidas na amostragem indicaram que nos voluntários onde o diâmetro predominante das partículas era menor, a fração depositada no pulmão foi maior, embora a concentração em massa fosse maior. Os resultados obtidos evidenciam a relevância do tamanho das partículas e composição química nos efeitos à saúde, a importância de medidas de exposição pessoal para estudos epidemiológicos em saúde e a viabilidade deste tipo de amostragem pessoal, porém para resultados mais significativos é recomendável a ampliação deste estudo.
The exposure to air pollutants in humans causes damage effects on health. Urban areas with high population density and high vehicular flows are critical regions. Air pollutants are omnipresent, and some level of exposure is inevitable, variable and dependent on a number of environmental and individual factors of each. This work aimed to measure and assess personal exposure to airborne particulate matter by individuals in Londrina with 30 voluntaries from 6 occupation classes. Using personal monitors the concentration of airborne particulate matter concentrations were measured in mass and number of particles in the inhaled air. Buccal cells of the volunteers were collected before and after exposure to the determination of genotoxicity by Comet assay. Sampling campaigns were carried out in winter with georeferenced routes that varied according to the routine of each individual. From concentrations measured, time of exposure and body weight the received doses were calculated individually. The mass concentrations (average of 8 hours) found in the volunteers ranged from 2.41 to 34.07 µg m-3 for MP1,0 and 23.11 to 11060.54 µg m-3 for PM10. The concentrations in number of particles found varied from 14 to 183 particles cm-3 to MP0,3; for PM2.5 they varied from 0.20 to 27 particles cm-3. Construction and Industry category showed the highest doses, while the administrative sector had the lowest. Using the Kruskal Wallis test was found a significant difference of DNA damage in classes Trade, Construction and Industry and Transportation, when these were compared with the control group (Administrative class). The model best suited to the data was the Negative Binomial, indicating positive association, mainly of the occupation class, number of particles with damage to DNA from buccal cells. The results of the lung deposition simulation from the concentrations obtained in the sample show that in volunteers where the predominant particle diameter was smaller, the fraction deposited in the lungs was higher, although the bulk concentration was higher. The results show the importance of particle size and chemical composition on the health effects, the importance of personal exposure measurements for epidemiological studies on health and the feasibility of this type of personal sampling, but for more significant results it is recommended the magnification of this study.
Ardiles, Leda Gabriela. "Risco à saúde atribuído à poluição do ar e variáveis meteorológicas na região metropolitana de Curitiba." Universidade Tecnológica Federal do Paraná, 2016. http://repositorio.utfpr.edu.br/jspui/handle/1/1907.
Full textEste trabalho visou determinar o risco atribuído à saúde da população pela exposição à poluição do ar e variáveis meteorológicas na Região Metropolitana de Curitiba (RMC) e com maior ênfase nas cidades de Araucária e Curitiba, no período de 2010 a 2014, nas faixas etárias de 0 a 9 anos, 10 a 19, 20 a 64, maiores de 64 anos e para todas as faixas etárias incluídas. Curitiba e Araucária são os maiores polos populacional e industrial do estado, respectivamente, com predominância de distintas fontes de emissão de poluentes. Neste estudo foi investigada as associações da qualidade do ar e variáveis meteorológicas com doenças circulatórias e respiratórias, calculado o Risco Relativo (RR) e o risco atribuído a saúde pelas concentrações de MP10 e O3. Os resultados indicaram que o modelo com distribuição Binomial Negativa foi o mais adequado aos dados e utilizado diferentemente da maioria dos estudos que usam Poisson. A partir dos coeficientes obtidos no modelo de regressão, foram calculados o risco relativo e o risco atribuído causados pelos poluentes do ar. O melhor ajuste de modelo (resíduo de Pearson mais próximo a um), se encontrou para o grupo de idosos com mais de 64 anos, em Araucária, para doenças circulatórias devido às concentrações de CO, e para internações respiratórias devido aos poluentes O3, SO2 e T-Tm, que é a temperatura diária menos a temperatura média de 2010 a 2014. Em Curitiba, o melhor ajuste ocorreu para o grupo com todas as faixas etárias para internações circulatórias devido ao MP10, e o grupo de 20 a 64 anos para internações respiratórias, para CO, O3 e T-Tm. As defasagens mais importantes ocorreram entre 3 a 7 dias após a exposição aos poluentes atmosféricos. Em Araucária, o RR mais significativo estatisticamente para internações circulatórias foi de 1,44635 em maiores de 64 anos para CO, e nas internações respiratórias foi de 2,4128 encontrado na faixa etária de 10 a 19 anos, também para o CO. Em Curitiba o RR mais significativo ocorreu para internações por doenças respiratórias com valor de 1,00315 na faixa de 0 a 9 anos para o MP10, de 1,14881 na faixa de 20 a 64 anos para CO, de 1,00329 entre 20 a 64 anos para O3, e de 1,13886 para maiores de 64 anos devido ao CO. Foram associadas 112 e 97 internações devido ao MP10, por doenças circulatórias e respiratórias na RMC no período de 2010 a 2014. Foram associadas 67 internações respiratórias, devido ao O3. Com a estimação destes valores, é possível avaliar o impacto que a poluição do ar na RMC representa para a saúde pública e que poderiam ser evitados e direcionados na prevenção dos efeitos adversos à saúde da população, na implantação de políticas e normas reguladoras para diminuição da emissão e concentração dos poluentes atmosféricos.
This study aimed to determine the risk attributed to human health by exposure to air pollution and meteorological variables in the Metropolitan Region of Curitiba (MRC). The greater emphasis is on the cities of Araucária and Curitiba, in the period from 2010 to 2014, for age groups of 0-9 years old, 10-19, 20-64, over 64 years, and another including all ages. Curitiba has the major populations and Araucaria has the major industrial centers of the state, with a predominance of different pollutant emission sources. This study investigated the associations of air quality and meteorological variables with circulatory and respiratory diseases, the relative risk (RR) and the risk assigned to health by concentrations of PM10 and O3. The results indicate that the model with Negative Binomial distribution is the most suitable, differently of most of the studies presented until now. The relative risk and attributable risk by air pollutants were calculated from the coefficients obtained in the regression model. The best adjustment was found for the group of people over 64 years in Araucaria, both for circulatory diseases due to CO, as for respiratory hospitalizations due to O3, SO2 and T-Tm. In Curitiba, the best adjustment of the model was for the group containing all ages for circulatory hospitalizations due to PM10, and the group of 20-64 years for respiratory hospitalizations for CO, O3 and T-Tm. The most significant lags were from 3 to 7 days after the exposure to air pollution. In Araucária the most statistically significant RR was 1.44635 for circulatory hospitalizations among people over 64 years due to CO. For respiratory hospitalizations, the RR was 2.4128, found in the age group 10 to 19 years, corresponding to the pollutant CO. The RR was also important in the group of 20-64 years for NO2, which was 1.00758. At Curitiba the most significant RR was by hospitalizations for respiratory diseases in the amount of 1.00315 to the group of 0-9 years due to PM10, of 1.14881 in the range of 20-64 years old for CO, of 1.00329 for 20-64 to O3, and 1.13886 for over 64 years old due to CO. 112 circulatory hospitalizations in the MRC were associated to PM10 and 97 respiratory hospitalization were associated with the PM10. 67 respiratory hospitalizations in the MRC were associated to O3. With the estimation of these values, it is possible to evaluate the impact in public health and how these costs could be avoided and focused on the prevention of adverse effects of public health, the implementation of regulatory policies and regulations to reduce the emission and concentration of air pollutants.
Méausoone, Clémence. "Etude en Interface Air-Liquide de la toxicité des Composés Organiques Volatils lors d’expositions répétées : Cas du toluène, de ses homologues et des émissions issues de son traitement catalytique." Thesis, Littoral, 2019. https://documents.univ-littoral.fr/access/content/group/50b76a52-4e4b-4ade-a198-f84bc4e1bc3c/BULCO/Th%C3%A8ses/Toxicologie/these_Meausoone_Clemence.pdf.
Full textToluene is a solvent widely used in manufacturing industries. It belongs to a family of volatile organic compounds (VOCs), many of which have adverse impacts on human health and are classified as carcinogenic, mutagenic or toxic for reproduction. Different measures have been implemented to reduce the emissions of toxic compounds, such as their replacement in the industry by less harmful compounds and/or reducing gas emissions at the source. In this context, the first objective of the research was to investigate the acute toxicity and the one after repeated exposure to toluene and its superior homologous solvents, which can be used as its substitution compounds, as well as its lower homologous on human bronchial epithelial cells using an air/liquid interface exposure device. The second objective was to assess the toxicity of gaseous effluents from the degradation of toluene by catalytic oxidation. For this purpose, BEAS-2B cells were exposed during 1 hour for 1, 3 or 5 days to benzene, toluene, xylene or mesitylene, and to the exhausts of catalytic oxidation of toluene. Toxic effects were evaluated through cytotoxicity, inflammatory response and gene expression of xenobiotic metabolism enzymes (XME). Exposure of BEAS-2B cells to toluene and its homologous compounds revealed the involvement of metabolic pathways specific to each compound. A significant increase in inflammatory marker response was also observed, with a higher concentration after cell exposure to benzene and to xylene compared to the other molecules. With regard to exposure to gaseous effluents from the catalytic oxidation of toluene, the late expression of genes involved in the metabolism of aromatic organic xenobiotics has made possible to highlight the presence of by-products, such as benzene or polycyclic aromatic hydrocarbons. In conclusion, the results obtained in this project show the interest of conducting repeated in vitro exposures to detect potential late effects, and the importance of toxicological validation of catalytic systems before scaling-up in industrial pilots
André, Paulo Afonso de. "Câmara de Topo Aberto, CTA: construção e uso para observação de potencial tóxico da poluição atmosférica urbana com bioensaios em plantas." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-24102007-134900/.
Full textOpen Top Chamber was modified to obtain a differential concentration on environmental pollution capable to be used on toxicological studies. Since urban aerosol constitutes a very complex chemical composition, with well known toxic action on health but requesting clarification about their biological mechanisms, the use of Open Top Chamber with low cost sentinel systems seems to be an alternative to detect acute toxic effects on such environment. Open Top Chamber was described on its dimensional and operational characteristics, and operated on a 60 days campaign. During this campaign the daily average concentration of fine particles was 28,6 ug/m3 and inside the Open Top Chamber it was obtained a reduction about 75% on such concentration. Tradescantia clone 4430, KU20 and pallida cv. Purpurea were placed inside and outside the chamber to evaluate bioassay response on each pollution concentration. The Trad-MCN bioassay detected a lower micronuclei count on plants inside the chamber (p=0,002). Clones were submitted to stamen hair mutation (Trad-SHM) and pollen mother cell abortion protocols, detecting also a lower effect on plants inside the chamber (p=0,007 for stamen hair mutation and p=0,041 for pollen mother cells abortion). Leaves of all spices were collected and submitted to X-ray fluorescence analysis. The titanium concentration was lower on samplers collected inside the chamber (p=0,049). The factorial analysis identified the presence of elements from soil and automotive sources with a lower concentration on samples collected inside the chamber. The combined use of Open Top Chamber with bioassay on urban environment is capable to detect acute effects on plants when submitted to the obtained particulate concentration reduction.
Lehmann, Catherine. "Le radon dans les habitations." Bordeaux 2, 1995. http://www.theses.fr/1995BOR2P072.
Full textSentissi, Maya. "Impact sanitaire de la pollution atmosphérique urbaine particulaire." Paris 5, 1999. http://www.theses.fr/1999PA05P032.
Full textPariselli, Fabrizio. "Evaluation in vitro de la nocivité des composés organiques volatils de l'air intérieur : développement d'une technique d'exposition dynamique." Paris 5, 2008. http://www.theses.fr/2008PA05P643.
Full textHundreds of chemicals like volatile organic compounds (VOCs) are present in the indoor environment and are suspected to cause acute and chronic adverse health effects. At present, there is a lack of substantial information on toxicological effects caused by VOC mixtures at low concentrations; therefore, new experimental approaches should be designed to detect biological effects at typical indoor air concentrations. The aim of this PhD thesis was the in vitro evaluation of biological effects of low concentrations of indoor air pollutants. Toluene, benzene and formaldehyde were selected as representing key contaminants in indoor non-occupational environments. Human derived cell cultures, representing VOCs target organs were exposed to low concentrations of these air pollutants by means of an original exposure device, named CULTEX®, which permits the application of the volatile compounds directly on cell surface at the air/liquid interface. The exposition of cell cultures to low concentrations of selected volatile compounds, alone and in mixture, were able to initiate different biological effects in terms of toxicity, inflammatory response, oxidative stress and DNA damage. Further studies were performed in order to exploit the peculiarities of the exposure device: i. E. The combination of physical (UV) and chemical agents and the use of atmospheres generated in a climatic room by common construction materials
Dendene, Marie-Aude. "Exposition au monoxyde de carbone et aux hydrocarbures aromatiques monocycliques : étude comparative des divers modes de déplacement urbain et recherche de biomarqueurs de l'air expiré." Paris 5, 1995. http://www.theses.fr/1995PA05P016.
Full textCarteret, Marion. "Evaluation de l’exposition des personnes aux polluants issus des chauffages d’appoint au pétrole." Thesis, Lille 1, 2012. http://www.theses.fr/2012LIL10005/document.
Full textUnvented kerosene space heaters were responsible for 49 cases of acute carbon monoxide poisoning in France in 2007. This kind of heater may also induce chronic intoxications, but no information on these is available. The aim of this work is the laboratory quantification of the gaseous emissions from recent models of kerosene space heaters and the preparation of an indoor air quality study in the Nord-Pas-de-Calais region. Two types of kerosene heaters (wick and injector) were tested in an 8 m3 instrumented environmental chamber. They emit mainly CO2, CO, NO, NO2, particles and some VOCs (especially formaldehyde, benzene, 1,3-butadiene). Emission factors depend upon the kind of heater and upon the type of fuel, and particularly on the fatty acid methyl esters and sulfur content. The accumulation of soot on the wick heater during use leads to a high increase of the CO emissions, and could be responsible for chronic and acute CO intoxications. Field measurements were undertaken to assess the effective exposure of users to pollutants emitted by kerosene space heaters in a complex environment. Kerosene space heater emissions were found to be the main source of pollution. This work will be applied to an epidemiological study on respiratory diseases caused by these heaters
Ndong, Awa. "Pollution de l'air extérieur et intérieur à Dakar (Sénégal) : caractérisation de la pollution, impact toxicologique et évaluation épidémiologique des effets sanitaires." Thesis, Littoral, 2019. http://www.theses.fr/2019DUNK0510/document.
Full textAir pollution is nowadays a major societal challenge, as much for its harmful consequences on human health as on the environment. The general objective of this work was to determine the level of Dakar air pollution, outdoor and indoor, and to assess its health impact in the urban population. Fine particles (PM₂.₅) and a coarser fraction (PM>₂.₅) sampled at an urban site in Dakar (HLM), characterized by high road traffic emissions, were compared with particles sampled at a rural area, Toubab Dialaw located about 40 km from Dakar. The physicochemical characteristics of samples revealed that PMs differ for their physical (surface area) and chemical properties (in terms of CHN, metals, ions, paraffins, VOCs and PAHs) that were 65 to 75 % higher in urban samples. In accordance with the different sources and different physical and chemical properties, the inflammatory response (TNF-α, IL-1β, IL-6, IL-8) and the oxidative damage (total carbonylated proteins and 8-OHdG) were found higher in bronchial BEAS-2B cells exposed to urban PMs. The air quality measurement campaign showed that the bus interior was more polluted with PM₁₀, CO, CO₂ and NO than the market and the urban and rural indoor sites. The comparison of air quality between indoor and outdoor sites revealed that, particularly in urban areas, indoor air quality may be worse than the corresponding outdoor one. Monitoring individual exposure to pollutants showed that housewives in urban and rural sites were more exposed than professionals to air pollution, confirming previous observations of potential higher individual exposure level to pollutants in indoor environment. However, exposure level of traders and bus drivers that would result from the exhaust of traffic with often old, poorly maintained and uncontrolled vehicles has to be taken into consideration due the higher levels of gaseous pollutants here reported
Badran, Ghidaa. "Pollution atmosphérique particulaire : caractérisation physico-chimique et comparaison des effets toxiques des fractions extractible et non-extractible des PM₂.₅ In-vitro evaluation of organic extractable matter from ambient PM₂.₅ using human bronchial epithelial BEAS-2B cells : Cytotoxicity, oxidative stress, pro-inflammatory response, genotoxicity, and cell cycle deregulation. Toxicity of fine and quasi-ultrafine particles : focus on the effects of organic extractable and non-extractable matter fractions. Toxicological appraisal of the chemical fractions of ambient fine (PM₂.₅-₀.₃) and quasi-ultrafine (PM₀.₃) particles in human bronchial epithelial BEAS-2B cells." Thesis, Littoral, 2019. http://www.theses.fr/2019DUNK0547.
Full textAir pollution and particulate matter (PM₂.₅) were classified as carcinigens (group 1) by the International Agency for Research on Cancer in 2013. This particulate fraction represents a complex mixture with a highly variable composition influencing the toxicity. However, few studies have determined the respective involvement of the different chemical fractions of PM in their toxic effects. In this work, fine particles (PM₂.₅₋₀.₃) and quasi-ultrafine particles (PM₀.₃) were sampled in an urban site located in Beirut (Lebanon). After performing the physicochemical characterization of these two types of particles, their toxic effects (global cytotoxicity, metabolic activation, genotoxicity, inflammation, oxidative stress, autophagy and apoptosis) were investigated on a human bronchial epithelial cell line (BEAS-2B). The analysis of the organic content revealed differences between the concentrations of polycyclic aromatic hydrocarbons (PAHs), as welle as oxygenated (O-PAH) and nitrated (N-PAH) congeners, respectively 43, 17 and 4 times higher in PM₀.₃ than in PM₂.₅₋₀.₃.The toxicological study was based on the comparison of the toxicity of the fine particles considered in their entirety (PM₂.₅₋₀.₃), the extracted organic fraction (OEM₂.₅₋₀.₃) and the fraction not extracted by the dichloromethane (NEM₂.₅₋₀.₃). In addition, the specific effects of the organic fraction extrated from the quasi-ultrafine particles (OEM₀.₃) were compared with those of the organic fraction extracted from the fine particles (OEM₂.₅₋₀.₃). Our results showed that all the studied fractions were able to induce at least one of the studied mechanisms. PM₂.₅₋₀.₃ was able to induce toxic effects greater than those induced by OEM₂.₅₋₀.₃ and NEM₂.₅₋₀.₃. The organic fraction extracted from the quasi-ultrafine particles (OEM₀.₃), richer in organic compounds and in particular in PAHs and other congeners, appeared to be responsible for deleterious effects globally greater than that extracted from the fine particles (OEM₂.₅₋₀.₃). The results of this work have brought new elements on the relative toxicity of the different fractions of the fine particles and underline the crucial role played by ultrafine particles, still too little studied
Gaimoz, Cécile. "Caractérisation expérimentale des sources de composés organiques volatils dans deux mégapoles contrastées : Paris et Pékin." Versailles-St Quentin en Yvelines, 2009. http://www.theses.fr/2009VERS0040.
Full textThis work aims at characterizing Volatile Organic Compounds VOC sources and variability in two contrasted megacities (Paris and Beijing). This study based on an important experimental work, which includes instrumental optimization and participation in two intensive fields campaigns performed in May-June 2007 in Paris and in August 2007 in Beijing. Following qualitative analysis of the data sets and using a receptor model based on the PMF method, seven and ten sources of VOC were extracted for Paris and Beijing respectively. Traffic emissions are major VOC local/regional sources contributing to 54% and 35% of emissions in Paris and Beijing respectively. However, continental or regional emissions are also playing a significant role in the measured concentrations and so in the strength of VOC sources at sites receptors. Thus Paris is highly influenced by emissions from north-eastern Europe under anticyclonic condition, and Beijing by regional emissions from the Hebei province, in the south-east sector. Finally, identified sources and their contributions are compared to emission inventory data. For Paris, these results show significant differences with the local emission inventory, and suggest an overestimation of solvent emissions in the inventory
Borgie, Mireille. "Étude des particules fines et ultrafines en suspension dans l'air au Liban : caractérisation physicochimique et évaluation des effets toxicologiques sur des cellules pulmonaires humaines BEAS-2B." Thesis, Littoral, 2014. http://www.theses.fr/2014DUNK0366/document.
Full textThe objectives of this study, one of the first conducted in Lebanon, were to acquire a better knowledge on the physico-chemical characteristics of atmospheric fine particles (FP or PM₂.₅₋₀.₃) and ultrafine ones (UFP or PM₀.₃), and to assess their potential toxicity. Particles were collected at two coastal sites between may and sept. 2011 at Sin El-Fil (urban site in Greater Beirut), and between sept. and oct. 2011 at Bejje (rural site). After sampling, FP and UFP were subjected to a physico-chemical characterization by quantifying their inorganic ions and elements, total carbon and organic compounds contents. Then, composite samples of FP and UFP were prepared in order to expose bronchial epithelial cells (BEAS-2B) in culture, and therefore to assess the underlying toxic mechanisms. Our results showed an influence of combustion sources especially for urban particles that are richer in total carbon, organic compounds, metals and secondary inorganic ions than rural ones. On the other hand, a more pronounced cytotoxicity was caused by UFP when compared to FP. In addition, epigenetic modifications and genotoxicity mechanisms, such as metabolic activation of organic compounds, changes in three microRNAs expression, telomerase activation and DNA breaks induction, which are potentially involved in the initiation and promotion of carcinogenesis, were induced by the two samples of FP, with a more pronounced effect of urban particles. Exposure of BEAS-2B cells to collected FP, especially urban ones, may therefore induce the transformation of lung cells to immortal cells, and consequently the initiation or the promotion of broncho-pulmonary carcinogenesis
Fougère, Bertrand. "Influence de l'âge et du tabac sur les mécanismes génotoxiques et épigénétiques précoces de cancérogénèse broncho-pulmonaire en réponse à la pollution particulaire urbaine." Thesis, Littoral, 2014. http://www.theses.fr/2014DUNK0377/document.
Full textRecently recognized as carcinogenic to human by IARC, air pollution and fine particulate matter (PM₂.₅) can be inhaled and could be retained into the lung or reach the systemic circulation. This can cause or worsen many diseases for which the elderly are often more sensitive. The PhD objective corresponds to the identification of the mechanisms of action involved in the modulation of carcinogenic potential of PM₂.₅, in connection with age or smoking status. PM₂.₅ were collected in Dunkerque, a French seaside city characterized by important industrial activities and heavy motor vehicle traffic. In order to estimate the influence of age, blood lymphocytes sampled from 90 patients from age classes (25-30, 50-55 and 75-80 years old) were ex vivo exposed to PM₂.₅ during 72 hours, before evaluation of telomerase activity and gene expression modulation of P16INK4A and MGMT. PM₂.₅ modulated telomerase activity and telomeres length in all age groups without any influence of age. P16INK4A gene expression increased significantly with age after exposure to PM₂.₅. Age could enhance MGMT gene expression after exposure to particles by decreasing the level of promoter methylation in the oldest group. Regarding the role of smoking status, 26 broncho-alveolar lavage were performed in smoker and non-smoker people. Macrophages were cultured with bronchial epithelial BEAS-2B cells before PM₂.₅ exposure (3 or 15µg/cm²; 72h). The telomerase activity and telomere length vary after exposure and the tobacco modify these parameters in BEAS-2B cells and alveolar macrophages. Methylation of P16INK4A and MGMT genes promoters and their expression are not modified in BEAS-2B cells. In alveolar macrophages, particles lead to a decrease of methylation of P16INK4A gene promoter. The smoking status seems also to increase methylation and to down-regulate expression of these two genes. In conclusion, it seems that the studied PM₂.₅ sample can induce ex vivo modifications described in the initiation and promotion of lung carcinogenesis. The age and smoking status may modulate the toxic effects of particles. Since lung cancer symptoms appear only at an advanced stage, our results could help in proposing new biomarkers of carcinogenesis allowing an early diagnosis to improve survival
Lepers, Capucine. "Pollution atmosphérique de proximité et toxicité respiratoire : recherche in vitro des mécanismes d'action toxique induits par des aérosols atmosphériques particulaires (PM₂.₅) industriels, urbains et ruraux." Thesis, Littoral, 2013. http://www.theses.fr/2013DUNK0352/document.
Full textFine airborne particulate matter (PM₂.₅) can be inhaled and retained in deep lung for long periods, leading to onset or exacerbation of cardio-respiratory diseases. However, the complex composition of PM₂.₅ makes difficult the study of their mechanisms of action. This work fits into a global approach aiming to identify the toxicity mechanisms involved in a putative PM₂.₅ carcinogenicity, in association with PM composition. We study six PM samples collected either under industrial, urban or rural area, in spring-summer 2008 or autumn-winter 2008-2009 seasons. Biological fraction analysis revealed numerous and diverse bacterial and fungal components. We carried out Ames tests revealing a high mutagenic potency for PM samples, presumably linked to their nitro-aromatic content. Based on previous cytotoxicity assays, we studied PM effect on bronchial epithelial cell line BEAS-2B, at two concentrations (3.75 and 15µg/cm²). We demonstrated gene induction of several xenobiotic metabolizing enzymes involved in polycyclic aromatic hydrocarbons metabolic activation. This was associated with an increase in their catalytic activity, leading to bulky DNA-adducts formation in exposed cells. Furthermore, PM₂.₅ lead to DNA single- and double-strand breaks, micronuclei formation, and disturbed telomerase activity. In addition to these genotoxic effects, our study revealed epigenetic alterations such as P16ᴵᴺᴷ⁴ᴬ promoter hypermehylation, histone 3 post-translational modifications, and miRNAs expression changes. Considering the impact of chemical composition on PM toxicity, organic compounds lead to the highest genotoxicity, whereas metals seem to induce more pronounced epigenetic modifications. Altogether, our results indicate that the studied PM₂.₅ samples, through cooperative action of organic and inorganic fractions, may lead in vitro to multiple alterations involved in initiation and promotion steps toward pulmonary carcinogenesis
Ly, Hamidou. "De New York à Dakar : pour une approche critique et méthodologique de la justice environnementale." Thesis, Paris 8, 2016. http://www.theses.fr/2016PA080089.
Full textThe theme of inequality and its corollaries, such as poverty, insecurity, exclusion or segregation, comes more and more from a perspective of justice and concern at the same time the spatial and environmental issues. As to mark the paroxysm of inequalities, the issue of environmental justice comes revive the debate over access to resources within the social and spatial categories, as well as the debate about the unequal distribution and impact of externalities caused by the use of these resources from an environmental point of view.From New York to Dakar, despite the differences in socio-cultural and economic contexts, the same phenomena of inequality to environmental conditions are observed with some differences in the forms of manifestations and interpretations. This work aims to reflect on the variations that can have these phenomena of environmental injustice in the city of Dakar from an approach developed in American cities, the cradle of the protest movements. This work aims to reflect on the variations that can have these phenomena of environmental injustice in the city of Dakar from an approach developed in American cities, the cradle of the protest movements.Thus, based on the analysis of environmental pollution linked to industrial pollution, it is to show the importance of "contextualization" in the study of environmental injustice in terms of socio-spatial dynamics but also perceptions of the said situations of injustice
Al, Zallouha Margueritta. "Étude prospective pilote des effets d'une exposition ex vivo de lymphocytes T humains à la pollution atmosphérique particulaire : recherche de biomarqueurs et influence de l'âge." Thesis, Littoral, 2017. http://www.theses.fr/2017DUNK0472/document.
Full textAtmospheric fine particulate matter (FP) are able to enter the lungs where some compounds can interact with lung cells and reach the bloodstream . Exposure to FP affects in particular susceptible populations such as the elderly. This thesis is part of a project aiming to identify the effects of FP on human T lymphocytes (LT) while attempting to determine biomarkers related to exposure and to evaluate the variation of the cellular response as a function of age. LT were isolated from blood samples of 91 volunteers belonging to three age groups (20-30, 45-55, 70-85 years) then exposed ex vivo for 72h to 45 µg/µl of FP collected in Dunkirk. The steps of isolation, purification and activation of LT were first optimized. Following the characterization of the sampled population, a homogeneous study population was selected (10 subjects/age class). We have demonstrated an induction of the genes coding for the enzymes involved in the metabolic activation of PAH identified in the PF sample. Characterization of the LT profile made it possible to propose a mixed th1/th2 profile cause by the exposure. Teh transcriptomic study of miRNAs revealed an overexpression of miR-124-3p involved in the regulation of several functions in the immune system and miR-1290 involved in several types of cancer. As for the influence of age, overexpression of the genes coding for the antioxidant enzymes (NQO1 and HMOX1), an increase in the concentration of cytokines (IL-4 and IL-13) as well as a modification of the expression profile of some miRNAs were noted on the elderly
Barnhisel, Taylor. "Carbaryl Exposure to Danio rerio Leads to Activation of the Aryl Hydrocarbon Receptor Pathway." Wittenberg University Honors Theses / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617979942441695.
Full textPeiffer, Julie. "Étude de la neurotoxicité d’un Polluant Organique Persistant chez le rat : effets à court et à long terme de l’inhalation répétée de fluorène sur le développement sensori-moteur du jeune et le comportement à l’âge adulte." Thesis, Vandoeuvre-les-Nancy, INPL, 2011. http://www.theses.fr/2011INPL103N/document.
Full textActually air pollution is ubiquitous due to the emission of chemical compound from many sources. In this context, Polycyclic Aromaric Hydrocarbons (PAH)related compounds are widely distributed in the air and have shown deleterious health effects.Fluorene was chosen as a representative compound of PAHs pollution. This work consisted in the evaluation of its neurotoxic effects in adult animals, exposed i.p., orally or by exposure nose-only. The inhalation model of exposure was then applied to the study of its effects on sensorimotor development and on behavioral activity of animals exposed in utero or during lactation.The results showed that fluorene is able to induce behavioral changes in adult animals exposed directly or indirectly on the level of anxiety and the locomotor activity, whereas no effect on learning and memory abilities has been observed. However, no defect on the development of motor and sensory functions was demonstrated. Furthermore, the analysis of the presence of the compound and three of its metabolites showed that the pollutant was able to cross the blood brain barrier and can be metabolized in the brain. Moreover, variations have been observed concerning behavioral and physiological responses between studies, showing that effects induced by fluorene are dependent on the way of administration, the level of contamination and the time of exposure.In conclusion, these results demonstrate in animals the behavioral toxicity of fluorene at levels of contamination corresponding to human cases of exposure, confirming so the risk of PAH exposure throughout life
Bandaly, Victor. "Etude de la persistance de virus sur les filtres des centrales de traitement d'air : influence des paramètres de procédé et impact sur la santé." Thesis, Rennes 1, 2017. http://www.theses.fr/2017REN1B038/document.
Full textAir pollution is one of the major public health problems of our century and especially of indoor air as we spend about 90% of our time in closed environments. Among pollutants bioaerosols have been poorly studied. However, epidemiological studies have already shown a relationship between bioaerosols and human health. The aim of this PhD work is to learn about respiratory viruses in closed environments via ventilation systems in order to study indoor air quality. At the end of state of the art of air pollutants, it is important to define those present in the air that need to be treated, ventilation systems, filtration processes by fibrous media and the processing methods being able to be implemented. The effects of viral bioaerosols on public health in indoor environments were discussed and drafted in a bibliographic review. The methodology of the study was to assess the fate of respiratory viruses, mengoviruses and adenoviruses, in a miniature experimental system similar to air treatment systems used in closed environments. The experimental system used was validated and the filter performance against viral aerosols was investigated. This study presented originality for the characterization and the fate of two non-enveloped respiratory viruses, mengovirus (RNA) and adenovirus (DNA), in indoor environments and their fate on fiber glass filter. This study showed the ability of viruses to pass through the filter and to remain infectious upstream and downstream the filter. There is scarce literature on this subject, and this project allowed us to add new relevant data on the persistence of respiratory viruses in indoor air and more precisely at the level of filters in air handling units
Cassien, Mathieu. "Etudes in vitro/in vivo de la toxicité de polluants atmosphériques. Implication du stress oxydant dans les mécanismes génotoxiques et sur la variation des paramètres biochimiques." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4703.
Full textEpidemiological studies have consistently reported that particulate matter in ambient air pollution is associated with increases in cardiopulmonary diseases and mortality. Because they can deeply penetrate lung tissue, reaching blood stream and organs, nanoparticles (NPs) are considered particularly harmful. Here, our foray into the specific mutagenicity and cytotoxicity of NPs focused on manufactured nano-CeO2 (a fuel additive) and NPs known to form in air from a variety of atmospheric toxicants (eg, combustion residues, pesticides). We first revealed a genotoxic effect of nanoCeO2 on human fibroblasts by a clastogenic mechanism following stimulation of the release of O2•-/H2O2 by NADPH oxidase and mitochondria. However, upon exposure of these cells to nM doses of 1-nitropyrene (a combustion byproduct) promotion of DNA damage involving an aneugenic mechanism occurred before a clastogenic effect was seen at µM doses. Second, using a home-made chamber equipped with an aerosol generator, we determined indices of oxidative stress and tissue damage in rats chronically inhaling toxicant NPs for 1-5 months at environmentally relevant doses. Long term exposure, even at low NPs doses, provoked systemic oxidative stress, lipid peroxidation, kidney damage, liver dysfunction, changes in lipid profile and occurrence of disorders of glucose tolerance. Moreover, a strong impairment of hemodynamic performance was evidenced in hearts from NP-exposed rats. By extending literature data of the in vitro toxicity of NPs to the in vivo situation, our study incriminates the nano-sized components of atmospheric particulate matter as a privileged vector of genotoxicity and cardiotoxicity
Melki, Pamela. "Health impact of airborne particulate matter in Northern Lebanon : from a pilot epidemiological study to physico-chemical characterization and toxicological effects assessment." Thesis, Littoral, 2017. http://www.theses.fr/2017DUNK0444/document.
Full textExposure to air pollution, especially fine particulate matter (PM₂.₅), remains a major health risk, mainly in the developing countries. Northern Lebanon is affected by several sources of anthropogenic, urban and industrial pollution. However, no studies have examined the impact of PM₂.₅ on public health in this region. In addition, it should be noted that the toxicity mechanisms of PM₂.₅ are not fully identified. The aim of this work is to study the composition and the health impact of the atmospheric particulates in Northern Lebanon. An epidemiological survey was performed and fine particles were extracted and characterized physico-chemically and toxicologically. This study was conducted in two sites, one of which is influenced by industrial activities. Perception and epidemiological survet, conducted in two areas in Northern Lebanon, rural and industrial (310 treatable questionnaires/area), showed a relationship between annoyance, respiratory diseases and living in proximity to industrial activities. Moreover, results confirmed the interest in conducting a toxicological study in this region. Hence, to contribute to fulfill the gap of knowledge about the pulmonary toxicity of particulate matter and the mechanisms of action involved in the carcinogenicity, the study of physicochemical characteristics and toxicological endpoints of PM₂.₅₋₀.₃ from both sites were performed. Physicochemical analyses of the collected particles evidenced similar characteristics in major species. In particular, we have shown slightly higher levels of PAHs and trace metals and up to 100 times higher dioxins concentrations at the vicinity of industries. Our results evidenced the influence of numerous combustion sources (diesel, gasoline, coal and biomass burning) ; waste combustion and other industrial processes are also suspected. A more pronounced genotoxic and mutagenic potential was evidenced after exposure to particles collected at the vicinity of industries when compared to the rural ones, using the Ames fluctuation test and SOS chromotest. The effects of the collected particles are probably related to their organic composition. In order to assess the underlying toxic mechanisms, human bronchial epithelial cells (BEAS-2B) were then exposed to different concentrations of the sampled PM₂.₅₋₀.₃. Genotoxicity mechanisms such as metabolic activation of organic compounds (CYP1A1) and consecutive DNA damages such as DNA strands breaks (yH2AX quantification by flow cytometry analysis and in-cell western assay) were induced by the two samples of PM₂.₅₋₀.₃ , with a more pronounced effect of industrial particles. Moreover, PM showed tendency to alter the DNA repair process (OGGI, NTH1, APE1, NUDT1, DNMT1, MGMT, XPA, XRRC1 gene expression and PARP1, DNMT1, OGG1 proteins expression). DNA repair mechanisms were repressed up to 48h of exposure to PM especially to the industrial influenced PM₂.₅₋₀.₃ and reactivated after 72h of exposure. The DNA damages involve bulky DNA adducts, oxidative stress damages, DNA strand breaks and methylation. These results suggest mutagenic, genotoxic and epigenetic mechanisms of action involved in the carcinogenicity of fine particles, partly related to their organic composition
Gaudreau, Jennifer A. "Traffic Related Air Pollution and Lung Function in Bilateral Lung Transplant Patients." 2018. https://scholarworks.umass.edu/masters_theses_2/643.
Full textScharber, Helen. "Three essays on racial disparities in infant health and air pollution exposure." 2011. https://scholarworks.umass.edu/dissertations/AAI3482725.
Full text"A comparison of particulate matter (PM101) in industrially exposed and non exposed communities." Thesis, 2008. http://hdl.handle.net/10413/556.
Full textThesis (MMed.)-University of KwaZulu-Natal, Durban, 2008.
"Measurement and health risk assessment of volatile organic air pollution in fenceline and control communities in south Louisiana." Tulane University, 2004.
Find full textSmith, Nadia. "Air quality monitoring with polar-orbiting hyperspectral infrared sounders : a fast retrieval scheme for carbon monoxide." Thesis, 2014. http://hdl.handle.net/10210/12282.
Full textThe Infrared Atmospheric Sounding Interferometer (lASI), operational in polar-orbit since 2006 on the European MetOp-A satellite, is the most advanced of its kind in space. It has been designed to provide soundings of the troposphere and lower stratosphere at nadir in a spectral interval of 0.25 em" across the range 645-2 760 em". Fine spectral sampling such as this is imperative in the sounding of trace gases. Since its launch, the routine retrievals of greenhouse, species from IASI measurements have made a valuable contribution to atmospheric chemistry studies at a global scale. The main contribution of this thesis is the development of a new trace gas retrieval scheme for IASI measurements. The goal was to improve on the global operational scheme in terms of the algorithm complexity, speed of calculation and spatial resolution achieved in the final solution. This schemedirectly retrieves column integrated trace gas densities at single field-of-view (FOV) from IASI measurements within a 10% accuracy limit. The scheme is built on the Bayesian framework of probability and based on the assumption that the inversion of total column values, as apposed to gas profiles, is a near-linear problem. Performance of the retrieval scheme is demonstrated on simulated noisy measurements for carbon monoxide (CO). Being a linear solution, the scheme is'highly dependent on the accuracy of the a priori. A statistical estimate of the a priori was computed using a principal component regression analysis with 50 eigenvectors. The corresponding root-mean-square (RMS) error of the a priori was calculated to be 9.3%. In general terms, the physical retrieval improved on the a priori, and sensitivity studies were performed to demonstrate the accuracy and stability of the retrieval scheme under a numberof perturbations. A full system characterization and error analysis is additionally preformed to elicidate the nature of this complex problem. The hyperspectral IASI measurements introduce a significant correlation error in the retrieval. The Absorption Line Cluster (ALC) channel selection method was developed in this thesis, to address the correlation error explicitly. When a first neighbour correlation factor of 0.71 is assumed in the measurement error covariance for the clusters of ALC channels, then most of the correlation error is removed in the retrieval. In conclusion, the total column trace gas retrieval scheme developed here is fast, simple, intuitive, transparent and robust. These characteristics together make it highly suitable for implementation in an operational environment intended for air quality monitoring on a regional scale.
"In vitro cytotoxicity of metal ions and roadside dust collected in Hong Kong." 2002. http://library.cuhk.edu.hk/record=b5895982.
Full textThesis (M.Phil.)--Chinese University of Hong Kong, 2002.
Includes bibliographical references (leaves 135-144).
Abstracts in English and Chinese.
Acknowledgements --- p.i
Abstract --- p.ii
Abbreviations --- p.vi
List of figures --- p.viii
List of tables --- p.xi
Contents --- p.xiii
Chapter 1 --- Introduction --- p.1
Chapter 1.1 --- General introduction --- p.1
Chapter 1.2 --- Roadside air pollution worldwide and in Hong Kong --- p.2
Chapter 1.2.1 --- Air quality in Hong Kong --- p.3
Chapter 1.3 --- Characteristics of particulate matter --- p.9
Chapter 1.4 --- Composition and sources of particulate matter --- p.11
Chapter 1.5 --- Toxic effects of particulate matter --- p.12
Chapter 1.5.1 --- Lung injury --- p.12
Chapter 1.5.2 --- Cardiovascular injury --- p.15
Chapter 1.5.3 --- Mutagenesis and carcinogenesis --- p.16
Chapter 1.6 --- Aims of my study --- p.16
Chapter 2 --- Toxic Effects of Heavy Metals Ions on Selected Cultured Cell-lines --- p.18
Chapter 2.1 --- Introduction --- p.18
Chapter 2.1.1 --- Metals --- p.18
Chapter 2.1.1.1 --- Cadmium --- p.22
Chapter 2.1.1.2 --- Chromium --- p.23
Chapter 2.1.1.3 --- Lead --- p.25
Chapter 2.1.1.4 --- Zinc --- p.26
Chapter 2.1.2 --- Metallothioneins --- p.28
Chapter 2.1.3 --- p53 --- p.31
Chapter 2.1.4 --- Tumor Necrosis Factor-alpha (TNF-α) --- p.32
Chapter 2.1.5 --- Aims of this chapter --- p.32
Chapter 2.2 --- Materials and methods --- p.35
Chapter 2.2.1 --- Reagents --- p.35
Chapter 2.2.2 --- Cultured Cell lines --- p.35
Chapter 2.2.2.1 --- PU5-18 --- p.36
Chapter 2.2.2.2 --- LL24 --- p.36
Chapter 2.2.2.3 --- HBE4-E6/E7 --- p.37
Chapter 2.2.3 --- Cytotoxicity assays --- p.37
Chapter 2.2.4 --- ELISA assays --- p.40
Chapter 2.2.4.1 --- ELISA assay ofp53 levels --- p.41
Chapter 2.2.4.2 --- ELISA assay of TNF-α levels --- p.43
Chapter 2.2.5 --- MT gene expression studies by Luciferase assay --- p.44
Chapter 2.2.5.1 --- PCR amplification --- p.44
Chapter 2.2.5.2 --- 5´ة End modification of PCR amplified DNA --- p.44
Chapter 2.2.5.3 --- Ligation of DNA fragment to linearized vector --- p.46
Chapter 2.2.5.4 --- E. coli. transformation by heat shock --- p.46
Chapter 2.2.5.5 --- PCR sequencing --- p.47
Chapter 2.2.5.6 --- Transfection of plasmid into HBE4-E6/E7 cells --- p.49
Chapter 2.2.5.7 --- Data analysis --- p.50
Chapter 2.3 --- Results and discussion --- p.51
Chapter 2.3.1 --- Cytotoxicity assays --- p.51
Chapter 2.3.2 --- Combination effects of metals on cytotoxicity --- p.61
Chapter 2.3.3 --- p53 --- p.65
Chapter 2.3.4 --- TNF-α --- p.68
Chapter 2.3.5 --- MT gene expression studies by Luciferase assay --- p.69
Chapter 2.4 --- Conclusion --- p.74
Chapter 3 --- Effects of Polycyclic Aromatic Hydrocarbons (PAHs) on Cultured Cell-lines --- p.75
Chapter 3.1 --- Introduction --- p.75
Chapter 3.2 --- Materials and methods --- p.79
Chapter 3.2.1 --- Reagents --- p.79
Chapter 3.2.2 --- Cell culture --- p.79
Chapter 3.2.3 --- AlamarBlue assay --- p.80
Chapter 3.2.4 --- EROD assay --- p.80
Chapter 3.3 --- Results and discussion --- p.84
Chapter 3.4 --- Conclusion --- p.88
Chapter 4 --- Chemical and Biological Assays on Roadside Dust --- p.89
Chapter 4.1 --- Introduction --- p.89
Chapter 4.1.1 --- Composition of particulate matter in Hong Kong --- p.89
Chapter 4.1.2 --- Metal contents of particulate matter in Hong Kong --- p.91
Chapter 4.1.3 --- Possible adverse health impacts of particulate matter --- p.94
Chapter 4.1.3.1 --- In vitro studies using different cell models --- p.94
Chapter 4.1.3.2 --- In vivo studies using rodents --- p.97
Chapter 4.1.3.3 --- Epidemiological studies --- p.98
Chapter 4.1.4 --- Aims of this chapter --- p.100
Chapter 4.2 --- Materials and methods --- p.101
Chapter 4.2.1 --- Sampling of roadside dust --- p.101
Chapter 4.2.2 --- Chemical analysis of roadside dust --- p.104
Chapter 4.2.2.1 --- Reagents --- p.104
Chapter 4.2.2.2 --- Total metal contents --- p.105
Chapter 4.2.2.3 --- Extractable metal contents --- p.105
Chapter 4.2.3 --- Biological assays --- p.105
Chapter 4.2.3.1 --- Cell models --- p.106
Chapter 4.2.3.2 --- Pretreatment of roadside dust --- p.106
Chapter 4.2.3.3 --- AlamarBlue assay --- p.106
Chapter 4.2.3.4 --- ELISA assays --- p.108
Chapter 4.2.3.5 --- Luciferase assay --- p.108
Chapter 4.3 --- Results and discussion --- p.110
Chapter 4.3.1 --- Total metal contents --- p.110
Chapter 4.3.2 --- Extractable metal contents --- p.113
Chapter 4.3.3 --- AlamarBlue assay --- p.116
Chapter 4.3.4 --- p53 --- p.122
Chapter 4.3.5 --- TNF-α --- p.122
Chapter 4.3.6 --- Luciferase assay --- p.126
Chapter 4.4 --- Conclusion --- p.129
Chapter 5 --- General discussion and conclusion --- p.130
Chapter 6 --- References --- p.135
Nunez, Yanelli. "The effect of air pollution on aggravation of neurodegenerative diseases: an analysis of long-term exposure to fine particulate matter and its components." Thesis, 2020. https://doi.org/10.7916/d8-cj8x-7534.
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