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Journal articles on the topic 'Air – Microbiologie'
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1
Squinazi, Fabien. "Microbiologic air contamination and building-associated illness." Aerobiologia 6, no. 1 (June 1990): 45–50. http://dx.doi.org/10.1007/bf02539043.
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Ash, C. "MICROBIOLOGY: Getting Enough Air to Survive." Science 296, no. 5571 (May 17, 2002): 1203d—1203. http://dx.doi.org/10.1126/science.296.5571.1203d.
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Rahardhiman, Aryatama, Ririh Yudhastuti, and R. Azizah. "Microbiology Indoor Air Quality at Hospital During the Covid19 Pandemic." JURNAL KESEHATAN LINGKUNGAN 12, no. 1si (September 30, 2020): 89. http://dx.doi.org/10.20473/jkl.v12i1si.2020.89-92.
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Introduction: Covid19 was an acute respiratory disease with fever, cough, and out of breath as the symptoms. WHO reported that until June 21st 2020, there were 8,708,008 cases were confirmed with 461,715 number of death (CFR 5.3%). In Indonesia, there were 45,891 cases were confirmed with 2,465 number of death (CFR 37%). People who were most at risk were people who physically close contact with the Covid19 patient, including health workers. The purpose of this study was to know the microbiology indoor air quality of Covid19 patient at Hospital before and during the pandemic. Method: The study design of this research was observational cross sectional. The study was done at a Hospital in East Java on December 2019 – June 2020. The sample of this research was a ward’s air in a Hospital. The research variable was the number of microbiology, temperature, and humidity of the ward of Covid19 patient that was measured 3 different points of measurement each rooms. Result and Discussion: The result showed that the average of the number of microbiology before the pandemic was about 46.31 CFU/m3 with the average of the temperature was 27.64°C and the humidity was 44.58%, while during the pandemic the number of microbiology in the air increased to 64 CFU/m3 with the average of the temperature was 27.77°C and the humidity was about 42.46%. Based on the statistic analysis, there were differences between the numbers of the microbiology before and during the pandemic in the ward of Covid19 patients (p value 0.00). Conclusion: The result showed that the number of the microbiology was increased before and during the pandemic although it was still under the quality standard. Increasing of Covid19 patient was the probably reason of the increasing the number of the indoor air microbiology. It was recommended the hospital requires to control the air quality of the treatment room by regulating air ventilation.
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Pramaningsih, Vita, Rusdi, Slamet Isworo, and Ratna Yuliawati. "Indoor Air Quality of Physical and Microbiological in Universitas Muhammadiyah Kalimantan Timur, Indonesia." Indonesian Journal of Environmental Management and Sustainability 6, no. 1 (March 21, 2022): 168–74. http://dx.doi.org/10.26554/ijems.2022.6.1.168-174.
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Indoor air quality is important for mental health and a comfortable environment inside the rooms. Poor indoor air quality has an impact to the sick building syndrome in occupants in the rooms. Physical and microbiological are factors that influence indoor air quality. The purpose of this study is to measure indoor air temperature, humidity, and microbiology in Universitas Muhammadiyah Kalimantan Timur in the morning and afternoon. The method used field measurement, microbiological laboratory analysis, and regression test by SPSS. High temperature and microbiology occur in the afternoon, humidity is not significant between morning and afternoon. Temperature and humidity were influenced by ventilation and the use of air conditioners in the rooms. Ventilation, the efficiency of air circulation, building type, maintenance of air conditioner, and occupant density are factors in getting clean and healthy indoor air quality to keep a comfortable environment. The statistical analysis resulted that temperature and humidity have not affected the indoor air microbiological increase.
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Fontana, Carla, Marco Favaro, and Cartesio Favalli. "How Liquid Based Microbiology Can Change the Workflow in the Microbiology Laboratories." Advances in Microbiology 03, no. 06 (2013): 504–10. http://dx.doi.org/10.4236/aim.2013.36067.
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Harjanto, Sri, and Raharjo Raharjo. "Peran Laminar Air Flow Cabinet Dalam Uji Mikroorganisme Untuk Menunjang Keselamatan Kerja Mahasiswa Di Laboratorium Mikrobiologi." METANA 13, no. 2 (December 1, 2017): 55. http://dx.doi.org/10.14710/metana.v13i2.18016.
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Keamanan dan keselamatan kerja di lingkungan laboratorium sangat dibutuhkan , seperti di laboratorium mikrobiologi. Untuk menunjang hal tersebut di atas peran Laminar air flow cabinet dirasa sangat penting akan keberadaannya karena mempunyai tingkat keamana dan keselamatan bagi pemakai yang lebih tinggi dibandingkan jika kita masih menggunakan peralatan sederhana seperti inkas. Lebih –lebih untuk penanganan bakteri yang bersifat patogen. Di laboratorium mikrobiologi ini melayani mahasiswa yang mengambil mata kuliah praktikum pendidikan juga mahasiswa yang sedang menyelesaikan penelitian Tugas Akhir. Dengan Laminar air flow cabinet ini didukung kedisiplinan serta bekerja sesuai peraturan tata tertib yang ada diharapkan bisa membantu menyelesaikan tugas dan bisa memberikan perlindungan serta rasa aman bagi mahasiswa tersebut. The role of laminar air flow cabinet in the test of microorganism to presurve work safetyin student microbiology laboratory Safety and safety in the laboratory environment is needed, as in the microbiology laboratory. To support the above, the role of Laminar air flow cabinet feels very important to its existence because it has a level of security and safety for users who are higher than if we still use simple equipment such as inkas. More-for the handling of bacteria that are pathogenic. In this microbiology laboratory serves students who take courses of educational practicum as well as students who are completing research Final Assignment. With Laminar air flow cabinet is supported by discipline and work according to existing rules of order are expected to help complete the task and can provide protection and a sense of security for the student.
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Rosmiaty, Rosmiaty, Andy Mizwar, Rizmi Yunita, and Erma Agusliani. "Kajian Laik Fisik Sanitasi Dan Kualitas Mikrobiologis Depot Air Minum (DAM) Dibawah Program Pembinaan Dan Pengawasan Dinas Kesehatan Kabupaten Hulu Sungai Utara." EnviroScienteae 15, no. 1 (April 29, 2019): 127. http://dx.doi.org/10.20527/es.v15i1.6333.
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Determining category of physical appropriateness of DWS under health office development and controlling program of Hulu Sungai Utara Regency, Determining Microbiology quality of DWA under health office development and controlling program of Hulu Sungai Utara Regency, analyzing the correlation of physical appropriateness and microbiology quality if DWS under health office development and controlling program of Hulu Sungai Utara Regency. This is a quantitative study using cross-sectional research design in which the research was conducted in a certain period of time, the location of research was in 10 service area of the public health center in 9 subdistricts under health office development and controlling program of Hulu Sungai Utara Regency. It was found that 30 DWS (Drinking Water Storehouse) is under health office development and controlling program of Hulu Sungai Utara Regency, DWS which categorized as physically not Appropriate (TLF) was 20 DWS (66,7%) and categorized as physically appropriate (LF) was 10 DWS (33,3%). Microbiology quality of Balangan, Tabalong, Negara, Tangkawang and Rantau Bujur Darat River were excess the standard of quality of class I intended for the drinking water source. Microbiology quality of water source (PDAM) from 30 samples, it was found that 14 samples (46,7%) contained Coliform and E. coli, there were 2 samples (6,7%) contained only Coliform. Quality of microbiology and water product of 30 DWS, it was found that 2 DWS contained Coliform, all water of DWS production did not contain E.coli. Statistical test of the correlation of physical appropriateness and microbiology quality of river water cannot be conducted because all samples have the same category which is TMS so that there was no difference in result data of category determination. There was no significant difference between physical appropriateness with microbiology quality of Coliform (p=0,260) and E.coli (p = 0,235) raw water of DWS. There was no significant correlation of physical appropriateness with microbiology quality of Coliform (p = 0,540) water product of DWS, meanwhile for statistical test for correlation of physical appropriateness and microbiology quality of E.coli of water product cannot be conducted because there was no difference in result data of category determination so that all samples has the same category.
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Rositasari, Dianori, Azizah R, Soedjajadi Keman, Mahmudah, Izmi Dwira Eriani, and Sri Rochana. "ANALYSIS OF MICROBIOLOGY QUALITY OF SURGICAL ROOM AIR BASED ON HOSPITAL TYPE AND ITS IMPACT ON THE ENVIRONMENT AND HEALTH IN EAST JAVA ON 2019." International Journal of Psychosocial Rehabilitation 24, no. 04 (February 29, 2020): 1230–43. http://dx.doi.org/10.37200/ijpr/v24i4/pr201094.
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Pamungkas, Oktofa Setia, Henny Ayu Nirwala, and Dina Mala Pardede. "Microbiology Factor Measurement as Indoor Air Quality Parameter in Public Space." BIOEDUKASI 17, no. 2 (October 7, 2019): 75. http://dx.doi.org/10.19184/bioedu.v17i2.14482.
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Nearly 90% of people spend their time in both private and public indoor spaces. Bank is one of the public indoor spaces accessible to the community, as well as a place for some workers spending time every day. This study was conducted in 6 banking sectors in Samarinda, East Kalimantan, focusing on the existence of microorganisms such as bacteria and fungi/mold. The purpose was to investigate the number of microorganisms, both bacteria and fungi, contained in indoor areas of several bank offices in Samarinda. The results showed that the number of bacteria and fungi at several sampling points in 6 offices were above the standard of Permenaker RI No. 5 the year of 2018 and Permenkes RI No. 48 the year of 2016, i.e.,>700 cfu/m3 for bacteria and >1000 cfu/m3 for fungi.
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Hidayah, Euis Nurul, Okik Hendriyanto Cahyonugroho, and Gabriela Veronica. "SOSIALISASI PENANGANAN KEGAGALAN RESIKO PENGOLAHAN AIR MINUM ISI ULANG DI KECAMATAN GUNUNG ANYAR SURABAYA." SELAPARANG Jurnal Pengabdian Masyarakat Berkemajuan 3, no. 2 (May 10, 2020): 156. http://dx.doi.org/10.31764/jpmb.v3i2.2053.
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ABSTRAKAdanya peluang bagi banyak industri untuk membuka depot air minum isi ulang (DAMIU), khususnya di Kecamatan Gunung Anyar, Surabaya. Isu yang muncul saat ini adalah rendahnya jaminan kualitas terhadap air minum isi ulang (AMIU) yang dihasilkan DAMIU. Jumlah koliform yang semakin besar menunjukkan kemungkinan bakteri patogen yang hidup di air yang terkontaminasi pencemaran. Meninjau permasalahan tersebut, maka kegiatan pengabdian masyarakat dilakukan dengan tujuan untuk menginformasikan kepada pengusaha DAMIU di Kecamatan Gunung Anyar tentang hasil pendataan teknologi pengolahan AMIU yang digunakan, menginformasikan hasil kualitas AMIU dari masing-masing DAMIU, menginformasikan risiko penyebab yang mempengaruhi kualitas AMIU dan memberikan rekomendasi upaya mengurangi risiko kegagalan produksi AMIU kepada pengusaha DAMIU. Sejumlah DAMIU menggunakan teknologi sinar UV sebesar 16 depot, teknologi sinar UV-Ozone sebesar 5 depot, teknologi RO sebesar 1 depot, sedangkan terdapat 1 depot tidak menggunakan teknologi desinfeksi. Analisa dari kegagalan kualitas air minum isi ulang pada parameter mikrobiologis (total koliform dan Eschericia coli). Risiko penyebabnya adalah karena sumber daya manusia, sumber air baku, metode, sarana dan prasaran, pengawasan dan evaluasi. Oleh karena itu perlu diberikan rekomendasi untuk mengurangi risiko kegagalan parameter mikrobiologis melalui waktu kontak UV atau Ozon minimal 4 menit, monitoring rutin perawatan unit pengolahan, menyusun standar operasional prosedur. Kata kunci: air minum isi ulang; risiko; eschericia coli; total koliform; desinfeksi ABSTRACTThere are opportunities for many industries to open refill drinking water stores (RDWS), especially in Gunung Anyar District, Surabaya. Some issues have been arised recently, such as issue of low quality assurance of drinking water produced by RDWS. The greater number of coliforms indicates the possibility of pathogenic bacteria that live in contaminated water. Reviewing these problems, community service activities were carried out with the aim of informing to RDWS owner in Gunung Anyar District about the results of data collection on RDW processing technology used, informing the results of RDW quality from each RDWS, informing the risk of causes affecting RDW quality and providing recommendations to reduce the failurd risk of RDW production to RDWS owner. Regarding disinfection technology, about 16 stores applied ultraviolet technology, UV-Ozone was used by stores, and the remain used RO technolog. Analysis of the failure of refill drinking water quality on microbiology parameters (total coliform and Eschericia coli). Those risk were arised due to human resources, raw water sources, methods, facilities and infrastructure, monitoring and evaluation. Therefore it is necessary to provide recommendations to reduce the risk of failure of microbiologyl parameters through UV or Ozone contact time of at least 4 minutes, routine monitoring of treatment units, preparing procedure operational standard. Keywords: refill drinking water; risk; eschericia coli; total coliform; disinfection
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Deguillaume, L., M. Leriche, P. Amato, P. A. Ariya, A. M. Delort, U. Pöschl, N. Chaumerliac, H. Bauer, A. I. Flossmann, and C. E. Morris. "Microbiology and atmospheric processes: chemical interactions of Primary Biological Aerosols." Biogeosciences Discussions 5, no. 1 (February 15, 2008): 841–70. http://dx.doi.org/10.5194/bgd-5-841-2008.
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Abstract. This paper discusses the influence of bioaerosols on atmospheric chemistry and vice versa through microbiological and chemical properties and processes. Several studies have shown that biological matter represents a significant fraction of air particulate matter and hence affects the microstructure and water uptake of aerosol particles. Moreover, airborne micro-organisms can transform chemical constituents of the atmosphere by metabolic activity. Recent studies have emphasized the viability of bacteria and metabolic degradation of organic substances in cloud water. On the other hand, the viability and metabolic activity of airborne micro-organisms depend strongly on physical and chemical atmospheric parameters such as temperature, pressure, radiation, pH value and nutrient concentrations. In spite of recent advances, however, our knowledge of the microbiological and chemical interactions of primary biological particles in the atmosphere is rather limited. Further targeted investigations combining laboratory experiments, field measurements, and modelling studies will be required to characterize the chemical feedbacks, microbiological activities at the air/snow/water interface supplied to the atmosphere.
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Deguillaume, L., M. Leriche, P. Amato, P. A. Ariya, A. M. Delort, U. Pöschl, N. Chaumerliac, H. Bauer, A. I. Flossmann, and C. E. Morris. "Microbiology and atmospheric processes: chemical interactions of primary biological aerosols." Biogeosciences 5, no. 4 (July 30, 2008): 1073–84. http://dx.doi.org/10.5194/bg-5-1073-2008.
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Abstract. This paper discusses the influence of primary biological aerosols (PBA) on atmospheric chemistry and vice versa through microbiological and chemical properties and processes. Several studies have shown that PBA represent a significant fraction of air particulate matter and hence affect the microstructure and water uptake of aerosol particles. Moreover, airborne micro-organisms, namely fungal spores and bacteria, can transform chemical constituents of the atmosphere by metabolic activity. Recent studies have emphasized the viability of bacteria and metabolic degradation of organic substances in cloud water. On the other hand, the viability and metabolic activity of airborne micro-organisms depend strongly on physical and chemical atmospheric parameters such as temperature, pressure, radiation, pH value and nutrient concentrations. In spite of recent advances, however, our knowledge of the microbiological and chemical interactions of PBA in the atmosphere is rather limited. Further targeted investigations combining laboratory experiments, field measurements, and modelling studies will be required to characterize the chemical feedbacks, microbiological activities at the air/snow/water interface supplied to the atmosphere.
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Li, Hui Xing, Bei Ni Li, Geng Geng, Wei Xiao, and Wei Wang. "Detection and Analysis of Microbial Contamination in Central Air Conditioning System of a University." Advanced Materials Research 610-613 (December 2012): 661–64. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.661.
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Objective: Test the wind pipe dust and microorganism pollution status in the central air conditioning system of public buildings in the teaching area.Analysis of microbial pollution of the related factors.Methods:Sampling 6 functionally different area in the teaching building,using the microbiology analysis method on air conditioning system of blower tube surface dust pollution of microorganism detection,and the use of TiO2 photo catalyst purification mechanism on microbial contamination control.Results:on the basis of"centralized air ventilation system in public places sanitary specification",teaching areas of central air conditioning system of dust amount met the requirement basically.Bacterial concentrations generally beyond the standard,fungal concentration accords with the standard.The Method using TiO2 photo catalysis sterilization was proposed. Conclusion:Teaching of regional air conditioning pipe inner product dust weight is relatively large.Microbial pollution is serious.It is necessary that cleaning air conditioning system timely and using TiO2 Photocatalyst purification mechanism for killing air duct surface dust microorganism.
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Amelia, Siska Rizki, Endah Ningrat Jati Prakoso, Ikhsan Ferdiyansah, Ridho Anwar Richo, and Yudhistira Bayu Kuncara. "Analisis Kadar Kesadahan Total Pada Air Sumur dan Air PDAM Metode Kompleksometri di Kaliabang Bekasi." Jurnal Sains Teknologi dalam Pemberdayaan Masyarakat 4, no. 2 (December 31, 2023): 115–20. http://dx.doi.org/10.31599/jstpm.v4i2.1927.
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Water is a natural resource that is very necessary for the life of many people. Good water is water that meets the requirements of physics, microbiology, chemistry. Chemically there is no water in the form of chemicals Arsenic, Iron, chloride, and hardness in the form of CaCO3. Hardness is a condition with excessive lime content in the water. Hardness with high concentrations consumed in the long term can be detrimental to health. Several factors that interfere with residents' wells are the presence of white sediment in the water which causes public health problems, and the frequent blockage of the pipeline used. The method used is the complexometric method, this kind of research is descriptive in nature. The results of checking the well water obtained Ca levels, namely 100 ppm and Mg, namely 61.44 ppm, while the results of checking tap water with Ca kadae were 150.2 ppm and Mg levels were 75.80 ppm. From the results of this study it can be concluded that well water and pump water can be used for consumption because they have a low hardness value.
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Dziurzynski, Mikolaj, Karol Ciuchcinski, Magdalena Dyda, Anna Szych, Paulina Drabik, Agnieszka Laudy, and Lukasz Dziewit. "Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity." Applied Sciences 10, no. 20 (October 13, 2020): 7128. http://dx.doi.org/10.3390/app10207128.
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There is no standardized protocol for the assessment of microbial air contamination in museums and other cultural heritage sites. Therefore, most museums conduct such assessments based on their own guidelines or good practices. Usually, microbial air contamination is assessed using only classical microbiology methods with the application of a single growth medium. Therefore, this medium should be carefully selected to limit any selective cultivation bias. Metabarcoding, i.e., a next-generation sequencing (NGS)-based method, combined with classical microbiological culturing was used to assess the effectiveness of various media applications in microbiological screening at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland). The obtained results indicated that when using a classical microbiology approach to assess the microbial air contamination at the museum, the selection of a proper growth medium was critical. It was shown that the use of rich media (commonly applied by museum conservators) introduced significant bias by severely underreporting putative human pathogens and the bacterial species involved in biodeterioration. Therefore, we recommend the use of other media, such as Frazier or Reasoner’s 2A (R2A) medium, as they could yield more diverse communities and recovered the highest number of genera containing human pathogens, which may be suitable for public health assessments.
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MacNeil, L., T. Kauri, and W. Robertson. "Molecular techniques and their potential application in monitoring the microbiological quality of indoor air." Canadian Journal of Microbiology 41, no. 8 (August 1, 1995): 657–65. http://dx.doi.org/10.1139/m95-091.
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Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This review discusses the shortcomings of current methods of identification and detection and focuses on the potential for molecular techniques in this emerging field. Probe techniques, restriction endonuclease analysis, karyotyping, and DNA and polymerase chain reaction fingerprinting methods available to detect and identify bacteria and fungi significant in the indoor air environment are discussed. Problems that may be encountered using these techniques are also considered. The authors have included a brief discussion on current air sampling techniques as well as adapting these techniques for use with molecular detection methods.Key words: indoor air microbiology, microbiological air sampling, molecular detection methods.
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Karaca, Çiğdem Tepe, Seniha Şenbayrak Akçay, Sema Zer Toros, Çağatay Oysu, Ayşegül Verim, Şaban Çelebi, and Sebahat Aksaray. "External auditory canal microbiology and hearing aid use." American Journal of Otolaryngology 34, no. 4 (July 2013): 278–81. http://dx.doi.org/10.1016/j.amjoto.2012.12.002.
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Khandelwal, Asha. "Aerobiology: Aspects and Prospects." Journal of Palaeosciences 57, no. (1-3) (December 31, 2008): 251–55. http://dx.doi.org/10.54991/jop.2008.242.
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Aerobiology is the study of all biologically significant materials that are transported in the atmosphere. Air-borne bioparticles are either beneficial or hazardous, affecting the health and economic prosperity of man on one hand and serving as bio-indicators on the other. Aerobiology has been used as a discipline and as well as a tool for other disciplines such as medicine (allergology - both human & animal diseases, immunology, occupational hygiene), agriculture (plant pathology, pest management, arthropod dispersal), forestry & gene ecology, meteorology, climatology, biometeorology, microbiology, biodeterioration, indoor air quality, air pollution, industrial aerobiology, cultural heritage, palaeobotany, etc. Aerobiologists should encourage closer collaboration among various disciplines in order to achieve a more meaningful outcome.
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Jirna, I. Nyoman. "ANALISIS KUALITAS FISIK DAN BAKTERIOLOGI AIR BERSIH PERUSAHAAN DAERAH AIR MINUM DESA SELANBAWAK TABANAN." Meditory : The Journal of Medical Laboratory 10, no. 1 (June 2, 2022): 8–15. http://dx.doi.org/10.33992/m.v10i1.1917.
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ABSTRACTIntroduction: PDAM water Selanbawak village is water used by people at Selanbawak Village for their daily needs such as drinking water. Drinking water qualify the requirements of physics, microbiology, chemistry and radioactivity (Permenkes 492 of 2010). In 2020 there occur a landslide disaster which cause damage to pipes, causing health problems such as diarrhea. Methods: The research used quantitative with a descriptive design using the simple random sampling technique of 179 populationsThe study was conducted to determine the quality of clean water from PDAM based on physical and bacteriological parameters from January to April 2021 at the homes of the residents of Banjar Selanbawak kaja, then physical and bacteriological examinations using the Most Probable Number (MPN) method were carried out at Panureksa Utama Laboratory. Results: The results of physical quality from 15 samples (100%) qualify the health requirements based on Permenkes RI No. 492 / MENKES / PER / IV / 2010. Meanwhile, examination of Coliform bacteria (67%) with the category fulfilling the health requirements and examination of Escherichia coli bacteria (73%) with the category fulfilling the requirements based on Permenkes RI No. 492 / MENKES / PER / IV / 2010. Conclusion: Based on these results, it can be concluded that most of the PDAM water still qualify health requirements.Keywords: Selanbawak, physical, bacteriology, PDAM water
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Carson, Christine F., and Timothy JJ Inglis. "Air sampling during flow cytometric analysis of unfixed bacterial suspensions; a risk assessment." Gates Open Research 1 (November 6, 2017): 2. http://dx.doi.org/10.12688/gatesopenres.12759.1.
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This study investigated airborne bacteria in a university research laboratory during operation of an acoustic-enhanced flow cytometer for antimicrobial susceptibility testing by sampling room air before, during and after flow cytometer use. Air sampling in a nearby clinical laboratory was conducted for comparison during the same period. The three species of bacteria undergoing analysis by flow cytometry were Klebsiella pneumoniae, Burkholderia thailandensis and Streptococcus pneumoniae. These species were not detected from multiple 1000 L air samples acquired in the confined research laboratory environment. The main cultured bacteria were skin commensal and environmental bacteria, presumed to have been disturbed or dispersed in laboratory air by personnel movements during routine laboratory operation. The concentrations of bacteria detected in air samples were reduced after laboratory cleaning measures were introduced and were lower than those in a diagnostic clinical microbiology laboratory located nearby on the same biomedical campus.
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Gillen, Alan Lee, Whitney O. Daycock, and Andrew Serafin. "High MRSA Carriage Rate among Nursing Microbiology Students." Advances in Microbiology 04, no. 13 (2014): 871–77. http://dx.doi.org/10.4236/aim.2014.413096.
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Ummah, Muhimatul, and Retno Adriyani. "Hygiene and Sanitation of Drinking Water Depot and Microbiology Quality of Drinking Water in Ngasem Primary Healthcare Area, Kediri, East Java." JURNAL KESEHATAN LINGKUNGAN 11, no. 4 (October 31, 2019): 286. http://dx.doi.org/10.20473/jkl.v11i4.2019.286-292.
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Air minum yang diproduksi oleh Depot Air Minum (DAM) merupakan alternatif pemenuhan kebutuhan air minum masyarakat. Kepraktisan dan harga yang relatif lebih murah jika dibandingkan dengan air minum dalam kemasan, menjadikan daya tarik tersendiri bagi masyarakat untuk mengkonsumsi air minum produksi DAM. Tujuan penelitian ini adalah untuk mendeskripsikan higiene penjamah, sanitasi DAM serta keberadaan E.Coli pada air minum produksi DAM di daerah peri-urban. Penelitian ini merupakan penelitian observasional dengan pendekatan potong-lintang. Sampel penelitian adalah seluruh DAM di wilayah kerja Puskesmas Ngasem, Kabupaten Kediri, Jawa Timur sebanyak 22 DAM. Pengumpulan data dilakukan melalui pengamatan menggunakan lembar observasi dan kuesioner terstruktur sebagai panduan wawancara. Dilakukan pemeriksaan laboratorium keberadaan E. Coli pada sampel air minum produksi DAM. Berdasarkan pengamatan, hanya terdapat 27,3% (6 dari 22) DAM memiliki kategori kondisi higiene penjamah yang baik, 63,6% (14 dari 22) DAM memiliki kategori kondisi sanitasi tempat yang baik, dan seluruh (22) DAM memiliki kategori kondisi sanitasi dan kelengkapan peralatan yang baik. Sebanyak 9,1% (2 dari 22) DAM, air minum produksinya terkontaminasi E.Coli. Disimpulkan bahwa kondisi higiene penjamah dan sanitasi tempat DAM perlu ditingkatkan. Disarankan agar pemilik DAM menyediakan fasilitas cuci tangan yang dilengkapi air mengalir dan sabun sehingga penjamah dapat selalu menjaga kebersihan dirinya. Peningkatan pengetahuan penjamah melalui kursus higiene sanitasi DAM juga perlu dilakukan.
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Gilbert, Yan, and Caroline Duchaine. "Bioaerosols in industrial environments: a reviewThis article is one of a selection of papers published in this Special Issue on Biological Air Treatment." Canadian Journal of Civil Engineering 36, no. 12 (December 2009): 1873–86. http://dx.doi.org/10.1139/l09-117.
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Air quality in workplaces is a main concern for industrial hygienists and occupational health specialists. Aerosolization of microorganisms or their by-products from contaminated material can significantly decrease the air quality and expose the workers’ health to potentially harmful effects. To identify the risks and elaborate safe exposure thresholds, the microbiology of air samples from industrial workplaces must be characterized, which means the airborne microorganisms must be identified and quantified. This review summarizes, for nonbiologist specialists, the principal sampling and analysis techniques for air sample characterization regarding microbiological contamination. A survey of the literature regarding levels of airborne bacteria, fungi, and viruses in agricultural industries, waste management plants, peat and wood processing facilities, and other industries is also provided. This review shows that very complex bioaerosol sources are present in industrial settings, and variable and hardly predictable biodiversity is expected in such environments.
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Buerke, Boris, Alexander Mellmann, Christoph Stehling, Johannes Wessling, Walter Heindel, and Kai Uwe Juergens. "Microbiologic Contamination of Automatic Injectors at MDCT: Experimental and Clinical Investigations." American Journal of Roentgenology 191, no. 6 (December 2008): W283—W287. http://dx.doi.org/10.2214/ajr.07.3954.
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Chen, Yi-Chun, Yin-Shiou Lin, Shu-Fang Kuo, and Chen-Hsiang Lee. "Air Sampling for Fungus around Hospitalized Patients with Coronavirus Disease 2019." Journal of Fungi 8, no. 7 (June 30, 2022): 692. http://dx.doi.org/10.3390/jof8070692.
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The risk of developing coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) depends on factors related to the host, virus, and treatment. However, many hospitals have modified their existing rooms and adjusted airflow to protect healthcare workers from aerosolization, which may increase the risk of Aspergillus exposure. This study aimed to quantitatively investigate airborne fungal levels in negative and slightly negative pressure rooms for COVID-19 patients. The air in neutral pressure rooms in ordinary wards and a liver intensive care unit with high-efficiency particulate air filter was also assessed for comparison. We found the highest airborne fungal burden in recently renovated slightly negative air pressure rooms, and a higher airborne fungal concentration in both areas used to treat COVID-19 patients. The result provided evidence of the potential environmental risk of CAPA by quantitative microbiologic air sampling, which was scarcely addressed in the literature. Enhancing environmental infection control measures to minimize exposure to fungal spores should be considered. However, the clinical implications of a periodic basis to determine indoor airborne fungal levels and further air sterilization in these areas remain to be defined.
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AS, Ezemba, Nwabisi CM, Udemezue IO, Ezemba CC, and Osuala OJ. "Microbial assessment of indoor air of the applied microbiology laboratory, Nnamdi Azikiwe University, Awka, Nigeria." Trends in Infection and Global Health 2, no. 1 (July 2, 2022): 14–21. http://dx.doi.org/10.24815/tigh.v2i1.24475.
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Air is made up of enormous number of microorganisms mainly fungi and bacteria spores. Their estimation is important as an indication of cleanliness of any particular environment. The present study was carried out to assess microorganisms in indoor air of the Applied Microbiology laboratory of Nnamdi Azikiwe University, Awka. Prepared plates of Sabouraud Dextrose agar (SDA), Nutrient agar (NA), and Blood agar were exposed for ten minutes for culturing of microorganisms. The NA and Blood agar plates were incubated at 37oC for 24h while the SDA plate was incubated at room temperature for 48h. A total of ten (10) microorganisms were isolated from the samples. These include six (6) bacteria and four (fungal) species. The bacterial isolates include; Staphylococcus aureus, Bacillus sp., Pseudomonas aeruginosa, Escherichia coli, Streptococcus sp., and Proteus sp. The fungal isolates include Aspergillus sp., Penicillium sp., Fusarium sp., and Rhizopus sp. The most frequently isolated bacteria were Staphylococcus aureus and Bacillus sp. with 25% and 35% occurrence. Proteus sp., Streptococcus sp., E. coli, and P. aeruginosa had 20%, 10%, 5%, and 5% percentage occurrences respectively. Aspergillus and Penicillium sp. were the most frequently isolated fungal isolates with 40% and 30% occurrence respectively. Fusarium sp. and Rhizopus sp. both had 20% and 10% occurrence respectively. With this result, attention must be given to control those environmental factors which favor the growth and multiplication of microbes in the indoor air of the laboratory and air sterilization should be carried out to safeguard the health of users and workers.
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Miller, Thomas E., and Glenne Findon. "Touch Contamination of Connection Devices in Peritoneal Dialysis-Aquantitative Microbiologic Analysis." Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 17, no. 6 (November 1997): 560–67. http://dx.doi.org/10.1177/089686089701700607.
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Objective To determine the level of bacterial contamination associated with touch contact of a connector set during peritoneal dialysis (PD). Design The experiment utilized a laboratory-based simulation of a bag exchange procedure. Deliberatetouch contamination of the connector set spike was followed by quantitative recovery of micro-organisms from the connector and, in some cases, the dialysis bag. Subjects Patients undergoing PD were used as the “test” group. Departmental secretarial and laboratory staff served as the comparative control group. Setting The patients were voluntary subjects from a PD outpatients unit and were tested in their own homes. Outcome The numbers of micro-organisms contaminating a connector set and entering the dialysis bag during a touch-contamination event were determined. Additionally we identified hand hygiene and, in particular, the care taken to dry the hands after washing as being highly relevant to microbial touch-contamination levels. Patient hand disinfection, as practised in most PD units, effectively reduced touch contamination to low levels. Results Touch contamination of a connector set with unprepared hands led to fewer than 100 micro-organisms translocating from fingers to the spike. If the hands were washed but not dried before touch contact was made, up to 4500 micro-organisms trans located to the connector set spike. Air-towel drying of washed hands before touch contact reduced the translocating numbers by 95% -99%. Hand disinfection, as routinely practiced by PD patients, reduced the bacterial numbers reaching the peritoneal cavity after touch contamination to <5. The range of micro-organisms isolated from the fingers of PD patients using hand disinfectants on a regular basis showed considerably more diversity than the control group. Conclusion Hand care prior to bag exchange has a major effect on touch-contamination levels. Accidental touch contact of connecting devices by unprepared hands using a PD-bag exchange procedure leads to the translocation of 500 micro-organisms or fewer to the connector device. If the hands are wet at the time of contact the number translocating can be as high as 4500. Hand drying with an air towel before touch contact reduces the numbers translocating by 95% -99%. Hand disinfection procedures carried out prior to bag exchange minimizes touch-contamination levels.
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Rocha, Danielle Ribeiro, and Gabriele Marisco. "Ateliê didático." Educitec - Revista de Estudos e Pesquisas sobre Ensino Tecnológico 9, jan./dez. (March 3, 2023): e209223. http://dx.doi.org/10.31417/educitec.v9.2092.
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Os microrganismos são importantes para a saúde humana e o ensino sobre esses organismos é capaz de favorecer o conhecimento sobre eles, de modo que as pessoas possam entender seus benefícios e agir em relação às doenças. Como abordar os conteúdos de microbiologia costuma ser desafiador para os professores, a formação continuada é uma ferramenta que contribui para o processo de ensino e aprendizagem. Dessa forma, o objetivo do presente artigo é apresentar o “Ateliê didático Reinventa docente: ensino de microbiologia” e evidenciar suas contribuições para a (re)construção de saberes e práticas docentes no ensino de microbiologia com ênfase em saúde na Educação Básica. O contexto da pesquisa-formação é um processo formativo online realizado com professores de Ciências e Biologia. Na coleta de dados foram utilizados diferentes instrumentos, como questionários e diário formativo, avaliados com a análise de conteúdo. Durante o processo formativo, os participantes relataram questões sobre o ensino de microbiologia, como a importância do conhecimento prévio e da relação dos microrganismos com o cotidiano, além dos desafios enfrentados para abordar conteúdos dessa área do conhecimento. Além disso, houve reflexões, aprendizados e descobertas, demonstrando que o processo formativo contribuiu com aportes teóricos e metodológicos que propiciaram o interesse em incorporar novas estratégias na prática pedagógica. Assim, evidencia-se que o desenvolvimento de processos formativos no âmbito da formação continuada pode contribuir com saberes para a constituição de novos sentidos e práticas no que tange ao ensino de microbiologia com ênfase em saúde.
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Y, Valentina, and Umadevi S. "Phenotypic Detection and Quality Assessment of Indoor Air-Borne Microorganisms Using Passive Air Sampling Technique (Settle Plate) at A Tertiary Care Teaching Hospital in Puducherry." Journal of Pure and Applied Microbiology 13, no. 1 (March 31, 2019): 241–45. http://dx.doi.org/10.22207/jpam.13.1.25.
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Bratakusuma, Trias, Zanuar Rifai, and Muhrofin Muhrofin. "Implementasi Web-Service dan Aplikasi Seluler Pada Sistem Pemantauan Proses Penjernihan Air Perusahaan Daerah Air Minum." Jurnal Sisfokom (Sistem Informasi dan Komputer) 7, no. 2 (September 30, 2018): 137. http://dx.doi.org/10.32736/sisfokom.v7i2.571.
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Clean water is a water that can be used for many household need. To produce a high quality water a supervision on its production needs to be done by pdam on a regular basis in order to qualify for the requirements such as physic, microbiology, chemistry, radioactive. This days reporting process that is on time and accurate has yet to be done, beside that there is potential of mistake from this reporting due to its conventional preparation process was still high. The purpose from this was to implement web service using RESTfull method server that are integrated with cellular app in smartphone with android platform so that the data reporting process can be done at the exact same time with serving it on the data sample in a graphic and table form. The data gathering technique that are being used in this research was observation technique, interview, documentation, literature review, and questionnaire. The method that are being used in this research was the Global extreme programming method (GXP). This research produce a supervision on water cleansing based on cellular app based on web service to support the data reporting that are done real time, so the possibility of mistake in the water processing process In PDAM Tirta Wijaya can be known as soon as possible
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Amir, Ariel, and Nathalie Q. Balaban. "Learning from Noise: How Observing Stochasticity May Aid Microbiology." Trends in Microbiology 26, no. 4 (April 2018): 376–85. http://dx.doi.org/10.1016/j.tim.2018.02.003.
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Van Hamme, Jonathan D., Ajay Singh, and Owen P. Ward. "Recent Advances in Petroleum Microbiology." Microbiology and Molecular Biology Reviews 67, no. 4 (December 2003): 503–49. http://dx.doi.org/10.1128/mmbr.67.4.503-549.2003.
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SUMMARY Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments.
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Yousefsaber, Fatemeh, Zeinab Naseri, and Amir Hosein Hasani. "A Short Review of Forensic Microbiology." Avicenna Journal of Clinical Microbiology and Infection 9, no. 2 (June 29, 2022): 88–96. http://dx.doi.org/10.34172/ajcmi.2022.14.
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Background: Microbial forensics is a multidisciplinary area, which has been recently considered an effective tool in forensic investigations. This growing field of forensics covers a wide spectrum of different branches of science, including biology, chemistry, physics, geology, mathematics, and computer sciences, leading to a practical approach that can be applied in several areas such as bioterrorist actions, environmental issues, emerging and reemerging diseases, as well as reliable trace evidence at a crime scene. Methods: The information has been gathered via Google Scholar using several keywords, including forensic microbiology, bioterrorism, forensic investigation, and trace evidence. The data were from reliable articles and books published over 50 years. This paper is a short review of forensic microbiology with a bioinformatics perspective to use in different fields such as the court. Results: It is known that using either microorganisms or their toxins is a low-cost potential tool with serious morbidity and mortality rates that can spread all around the world by food or water supplies or even through the air, making them a perfect candidate bioweapon with minimum traceability. Studies have indicated that environmental conditions plus biological and abiotic factors would affect the following analysis and the final validation, which is an essential step in the forensic investigation due to its highly effective role in the court vote. To face different challenges, law enforcement has the infrastructure for attribution and deterrence (e.g., following the exact microbial forensics program) so that it can be used in court. Developing more reproducible, sensitive, and accurate methods, preparing a wide reliable database, and devoting the right amount of budget will help improve the whole forensic procedure in the legal system. Conclusions: The current paper is a short review of how forensic scientists can use microbial features on a crime scene to clarify and enhance the procedure to solve different criminal cases.
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Robertson, Charles E., Laura K. Baumgartner, J. Kirk Harris, Kristen L. Peterson, Mark J. Stevens, Daniel N. Frank, and Norman R. Pace. "Culture-Independent Analysis of Aerosol Microbiology in a Metropolitan Subway System." Applied and Environmental Microbiology 79, no. 11 (March 29, 2013): 3485–93. http://dx.doi.org/10.1128/aem.00331-13.
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ABSTRACTThe goal of this study was to determine the composition and diversity of microorganisms associated with bioaerosols in a heavily trafficked metropolitan subway environment. We collected bioaerosols by fluid impingement on several New York City subway platforms and associated sites in three sampling sessions over a 1.5-year period. The types and quantities of aerosolized microorganisms were determined by culture-independent phylogenetic analysis of small-subunit rRNA gene sequences by using both Sanger (universal) and pyrosequencing (bacterial) technologies. Overall, the subway bacterial composition was relatively simple; only 26 taxonomic families made up ∼75% of the sequences determined. The microbiology was more or less similar throughout the system and with time and was most similar to outdoor air, consistent with highly efficient air mixing in the system. Identifiable bacterial sequences indicated that the subway aerosol assemblage was composed of a mixture of genera and species characteristic of soil, environmental water, and human skin commensal bacteria. Eukaryotic diversity was mainly fungal, dominated by organisms of types associated with wood rot. Human skin bacterial species (at 99% rRNA sequence identity) included theStaphylococcusspp.Staphylococcus epidermidis(the most abundant and prevalent commensal of the human integument),S. hominis,S. cohnii,S. caprae, andS. haemolyticus, all well-documented human commensal bacteria. We encountered no organisms of public health concern. This study is the most extensive culture-independent survey of subway microbiota so far and puts in place pre-event information required for any bioterrorism surveillance activities or monitoring of the microbiological impact of recent subway flooding events.
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Arinze S Ezemba, C. M Nwabisi, I. O Udemezue, Oluchi Judith Osuala, and Chinyere Constance Ezemba. "Case report: Microbial assessment of indoor air of the applied microbiology laboratory, Nnamdi Azikiwe University, Awka, Nigeria." International Journal of Frontline Research in Multidisciplinary Studies 1, no. 1 (July 30, 2022): 001–7. http://dx.doi.org/10.56355/ijfrms.2022.1.1.0025.
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Air is made up of enormous number of microorganisms mainly fungi and bacteria spore. Their estimation is important as an indication of cleanliness of any particular environment. The present study was carried out to assess microorganisms in indoor air of Applied Microbiology laboratory of NnamdiAzikiwe University, Awka. Prepared plates of Sabouraud Dextrose agar (SDA), Nutrient agar (NA), and Blood agar were exposed for ten minutes for culturing of microorganisms. The NA and Blood agar plates were incubated at 37oC for 24h while the SDA plate was incubated at room temperature for 48h. A total of ten (10) microorganisms were isolated from the samples. These include six (6) bacteria and four (fungal) species. The bacterial isolates include; Staphylococcus aureus, Bacillus sp., Pseudomonas aeruginosa,Escherichia coli, Streptococcus sp.and Proteus sp. The fungal isolates include Aspergillus sp., Penicillium sp., Fusarium sp. and Rhizopus sp. The most frequently isolated bacteria were Staphylococcus aureus and Bacillus sp. with 25% and 35% occurrence. Proteus sp., Streptococcus sp., E. coli. and P. aeruginosa had 20%, 10%, 5% and 5% percentage occurrence respectively. Aspergillus and Penicillium sp.were the most frequently isolated fungal isolates with 40% and 30% occurrence respectively. Fusarium sp. and Rhizopus sp. both had 20% and 10% occurrence respectively. With this result, attention must be given to control those environmental factors which favor the growth and multiplication of microbes in indoor air of the laboratory and air sterilization should be carried out to safeguard the health of users and workers.
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Gde Mahayana, Rakyan Agung. "PEMODELAN KUALITAS AIR TANAH OLEH AIR LIMBAH DOMESTIK MENGGUNAKAN ARCGIS DI DESA KERTALANGU." Jurnal Riset Rekayasa Sipil 7, no. 2 (April 8, 2024): 78. http://dx.doi.org/10.20961/jrrs.v7i2.85898.
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<p><em>The community living in Kesiman Kertalangu Village, East Denpasar, Denpasar City, Bali, has a few open shallow wells at home. Well water is used for daily activities such as washing, washing vehicles, and watering plants. The main source of water is groundwater, which is found in rocks below the earth's surface. The availability and movement of groundwater depends on the geological conditions of the area.</em><em> </em><em>Groundwater that is used as a source of community life must meet good water quality, so the purpose of this study was to determine the condition of groundwater during the study. The samples used for groundwater testing were 30 wells spread across this village. The methods used were interviews with the local community, field testing of well water samples (smell and temperature tests), laboratory tests (pH, TSS, TDS, DHL and microbiology) and modeling analysis of all research data results using the ARCGIS application. The research results obtained were field tests such as odor tests and temperature tests that passed the clean water requirements because they met the standards set by the Ministry of Health. laboratory tests such as the TDS, TSS and DHL tests have met the requirements as clean water because the number of solid particles dissolved in water and gas is below the specified limits, and the amount of suspended solids in water is also below the limits. Microbiological test results showed that several points were quite polluted by E.Coli and Total Coliform bacteria caused by the distance between the well points and the septic tank in people's homes.</em></p>
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Morris, C. E., D. C. Sands, M. Bardin, R. Jaenicke, B. Vogel, C. Leyronas, P. A. Ariya, and R. Psenner. "Microbiology and atmospheric processes: an upcoming era of research on bio-meteorology." Biogeosciences Discussions 5, no. 1 (January 15, 2008): 191–212. http://dx.doi.org/10.5194/bgd-5-191-2008.
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Abstract. For the past 200 years, the field of aerobiology has explored the abundance, diversity, survival and transport of micro-organisms in the atmosphere. Micro-organisms have been explored as passive and severely stressed riders of atmospheric transport systems. Recently, an interest in the active roles of these micro-organisms has emerged along with proposals that the atmosphere is a global biome for microbial metabolic activity and perhaps even multiplication. As part of a series of papers on the sources, distribution and roles in atmospheric processes of biological particles in the atmosphere, here we describe the pertinence of questions relating to the potential roles that air-borne micro-organisms might play in meteorological phenomena. For the upcoming era of research on the role of air-borne micro-organisms in meteorological phenomena, one important challenge is to go beyond descriptions of abundance of micro-organisms in the atmosphere toward an understanding of their dynamics in terms of both biological and physico-chemical properties and of the relevant transport processes at different scales. Another challenge is to develop this understanding under contexts pertinent to their potential role in processes related to atmospheric chemistry, the formation of clouds, precipitation and radiative forcing. This will require truly interdisciplinary approaches involving collaborators from the biological and physical sciences, from disciplines as disparate as agronomy, microbial genetics and atmosphere physics, for example.
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Lettinga, G. "Digestion and degradation, air for life." Water Science and Technology 44, no. 8 (October 1, 2001): 157–76. http://dx.doi.org/10.2166/wst.2001.0489.
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Anaerobic degradation of dead biomass is a natural gasification process, an anaerobic crematorium producing a very useful end-product composed of methane and carbon dioxide, generally polluted with small amounts of some malodorous and quite toxic volatile S-compounds. It leads to the production of essential building elements for new life. This exciting field became my faith, vision, hope and expectation. This paper intends to present a reflection of more than three decades of research, teaching and advertisement in the field of sustainable environmental protection technologies, particularly of systems based on anaerobic digestion and the biological sulphur cycle. Considerable progress has been made during these decades worldwide, both in the basic understanding of the various processes and concepts, but also in the implementation of these systems, despite the fact that particularly the implementation frequently proceeded very laboriously. The difficulties certainly can no longer be attributed to technological limitations and/or insufficient understanding of the microbiology and chemistry only, but mainly to the frustrating social rigidity and short-term self-interest in all sectors of our society. By combining anaerobic processes with other microbiological degradation or transformation processes, like those based on the biological sulphur cycle, micro-aerobic and conventional aerobic and anoxic processes, ideal conditions can be created to valorise residues (wastes) from domestic, industrial and agricultural origin. It is simply not just ‘technology’, but also a route to achieve more sustainability and justice in society. It is a fight against conservative establishments. Decomposition, disintegration disbandment, it also stands for deliverance and liberation, space and air for continuation of life.
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Laxmi, Rajamanickam Venkata, A. Ramya, S. Vanaja, and Payala Vijayalakshmi. "Microbiological Surveillance of Hospital Environment in Chevella, India." Journal of Pure and Applied Microbiology 15, no. 3 (August 4, 2021): 1449–54. http://dx.doi.org/10.22207/jpam.15.3.38.
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Microbiological air contamination in operation theatres (OTs) and labor rooms (LRs) is a major risk factor for surgical site infection. Routine monitoring in vulnerable areas such as OTs and LRs should always be performed as part of infection control to evaluate the contamination by microorganisms and monitor for the presence of nosocomial agents. The present study is aimed to isolate and identify various pathogens in a hospital environment. The research was performed in the Bacteriology lab, Microbiology Department, Dr.Patnam Mahender Reddy Institute of Medical Sciences, Chevella, Ranga Reddy district, Hyderabad, India between November 2017 and November 2020 for a period of 3 years. Surface swabbing and settle plate techniques were two sampling techniques used in the analysis. A total of 3492 samples were collected from various hospital surface sites and 5 OTs and LR sites and equipment via the swab technique, while a Petri plate gravitational settling (passive) sampling method was selected for the collection of air samples and all these samples were properly transferred to the microbiology laboratory and processed by standard microbiological protocols. A total of 3492 surface swabs were taken from 5 OTs, LR sites and equipment from the hospital. Out of these 294 (8.42%) were culture positive and 3198 (91.58%) were culture negative. Among 294 microbial isolates, the highest number was reported from Bacillus spp. 212 (72.11%) and least number was from Pseudomonas spp. 6 (2.04%). Through air sampling methods, bacterial isolates were isolated from OTs and LR of various clinical departments and it was found that the highest bacterial count was reported from general surgery (677 CFU/m3) followed by orthopaedics (585 CFU/m3). Most of the microbial isolates isolated from OTs and LR of clinical departments found be species belonging to Bacillus spp. and Coagulase-negative Staphylococci (CoNS). In developing countries, routine indoor air quality management in healthcare facilities needs to be constantly monitored and appropriate measures are taken to detect and prevent acquired infections in hospitals. Settle plate methods for air and surface swabbing, also in resource-limited settings, are very useful, simple and cost-effective techniques for OT and LR monitoring.
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Cash, Phillip. "Proteomics in medical microbiology." Electrophoresis 21, no. 6 (April 1, 2000): 1187–201. http://dx.doi.org/10.1002/(sici)1522-2683(20000401)21:6<1187::aid-elps1187>3.0.co;2-f.
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Sari, Indah, Robiyatul Adawiyah, and Nurhidayanti Nurhidayanti. "PERBEDAAN HASIL KADAR FORMALIN PADA SAMPEL TAHU YANG DIRENDAM AIR HANGAT DAN AIR GARAM MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS." JURNAL MEDIA KESEHATAN 15, no. 2 (December 27, 2022): 140–53. http://dx.doi.org/10.33088/jmk.v15i2.857.
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Problems: Formalin Examination is use to determine of presence or absence of harmful substances or preservatives in samples tested or examined on food, especially tofu. The Aim Of The Research: To determine the difference in the results of formaldehyde levels in tofu samples soaked in warm water and salt water using UV-Vis Spectrophotometry method. Research Method: The type of research used was a pure experiment, which was conducted at the Laboratory of Chemistry and Microbiology of Agricultural Products, Department of Agricultural Technology, Faculty of Agriculture, Unsri Palembang. The sample used was tofu and the number of careful samples was 18 tofu samples using the total sampling technique. The Result: Based on the results of examining the formalin content in tofu samples soaked in warm water for 15 minutes using the UV-Vis Spectrophotometry method with the A & E Visible Spectrophotometer yielded an average of 0.56 mg/L while the results of examining formalin levels in tofu samples soaked in salt water produces an average of 0.74 mg/L. Data analysis using the Independent Sample T-Test test obtained a significant value for the warm water treatment p = 0.395 and a significant value for the salt water treatment p = 0.397. Conclutions: There are differences in the results of formalin levels in tofu samples soaked in warm water and salt water using UV-Vis Spectrophotometry method.
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Gautam, Rupesh, Maria Isabel Atienza, Maika Noda, and Mariaem Andres. "Non-tuberculous Mycobacterium Disease: Radiologic Manifestation in an Infant presenting with Respiratory Distress." Nepalese Journal of Radiology 5, no. 1-2 (December 14, 2017): 13–20. http://dx.doi.org/10.3126/njr.v5i1-2.18739.
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Non-tuberculous mycobacterium (NTM) comprises distinct group of organisms with lymphadenitis and pulmonary infection as the common manifestation. The diagnosis of pulmonary disease is based on clinical manifestations, radiologic findings and microbiologic culture. The classic NTM infection may be indistinguishable from pulmonary TB. Non-classic infection has predilection to the middle lobe and lingula unlike tuberculosis which is commonly seen in the upper lobes. The disease may also present as hypersensitivity pneumonitis with ground glass like opacities, centrilobular nodules and air trapping on imaging. The knowledge of imaging manifestations of NTM will aid in timely diagnosis and treatment of the disease.Nepal Journal of Radiology Vol.5(1-2) 2015: 13-20
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Surahmaida, Surahmaida, and Floreta Fiska Yuliarni. "Pengaruh Air Cucian Beras dan Air Mikroorganisme Lokal Terhadap Mortalitas Rayap Tanah." JURNAL SAINS TEKNOLOGI & LINGKUNGAN 9, no. 4 (December 27, 2023): 621–26. http://dx.doi.org/10.29303/jstl.v9i4.465.
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Subterranean termites (Coptotermes curvignathus) are dangerous termites compared to other types of termites because thay cause damage to crops in plantations and agriculture and have a wide distribution. The use of chemical pesticides causes pests to become resistant and harmful to the environment and health. Botanical pesticides are used as an alternative solution that is environmentally friendly by utilizing leri water and MOL water. This research aims to determine the effect of leri water and lokal microorganism ater (MOL) to control subterranean termites and to determine the optimal formulation as an anti-termite. The research method using 5 formulations of botanical pesticide, namely 100% leri water, 100% MOL water, 50% leri water : 50% MOL water, 75% leri water : 25% MOL water, 25% leri water : 75% MOL water and control (without formulation). Each test container contains 5 soldier termites and 20 worker termites and is given a filter paper test that has been soaked with a predeterminated formulation. Each treatment was replicated 3 times and observed every 24 hours for 72 hours. The results showed that all formulations had no effect on subterranean termite mortality.
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Carson, Christine F., and Timothy JJ Inglis. "Air sampling to assess potential generation of aerosolized viable bacteria during flow cytometric analysis of unfixed bacterial suspensions." Gates Open Research 1 (February 27, 2018): 2. http://dx.doi.org/10.12688/gatesopenres.12759.2.
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This study investigated aerosolized viable bacteria in a university research laboratory during operation of an acoustic-assisted flow cytometer for antimicrobial susceptibility testing by sampling room air before, during and after flow cytometer use. The aim was to assess the risk associated with use of an acoustic-assisted flow cytometer analyzing unfixed bacterial suspensions. Air sampling in a nearby clinical laboratory was conducted during the same period to provide context for the existing background of microorganisms that would be detected in the air. The three species of bacteria undergoing analysis by flow cytometer in the research laboratory were Klebsiella pneumoniae, Burkholderia thailandensis and Streptococcus pneumoniae. None of these was detected from multiple 1000 L air samples acquired in the research laboratory environment. The main cultured bacteria in both locations were skin commensal and environmental bacteria, presumed to have been disturbed or dispersed in laboratory air by personnel movements during routine laboratory activities. The concentrations of bacteria detected in research laboratory air samples were reduced after interventional cleaning measures were introduced and were lower than those in the diagnostic clinical microbiology laboratory. We conclude that our flow cytometric analyses of unfixed suspensions of K. pneumoniae, B. thailandensis and S. pneumoniae do not pose a risk to cytometer operators or other personnel in the laboratory but caution against extrapolation of our results to other bacteria and/or different flow cytometric experimental procedures.
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Prastowo, Bambang Widyo, Manja Meyky Bond, and Betutu Senggagau. "Perbandingan Sistem Resirkulasi dan Air Mengalir Untuk Pembesaran Lobster Pasir (Panulirus Homarus): Kajian Dinamika Kualitas Air." Barakuda 45: Jurnal Ilmu Perikanan dan Kelautan 4, no. 1 (April 28, 2022): 12–23. http://dx.doi.org/10.47685/barakuda45.v4i1.205.
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Aquaculture with the Recirculation Aquaculture System (RAS) is a unique engineered ecosystem that minimizes environmental disturbances by reducing the discharge of nutrient pollution through reuse of water used in fish farming. This activity was conducted at LP2IL Serang field test facility starting in June-November 2020. In this test, the RAS system used for growing of sand lobster uses the stages of purification: sedimentation, physical filtration, biological filtration, water disinfection with UV irradiation, removal of CO2, clean water reservoirs and sand lobster rearing tanks. Water quality parameters tested: a) physics: DO, pH, temperature and salinity are measured every day; b) chemistry: ammonia (TAN and UIA), nitrite, nitrate and phosphate are measured every two weeks. If from the UIA value calculation, the value is above the quality standard (> 0.05 mg / L) then added molasses (26.02% organic C) with a concentration of 64 gr / gr TAN; c) microbiology: total bacteria and Vibrio sp. counting. From the results of this study it is known that RAS can maintain stable water quality for a relatively long time. High availability of organic matter (from leftover feed, feces and addition of molasses) can promote the growth of nitrifying bacteria. The "maturation" of the nitrifying bacteria in the biofilter compartment takes about 6 weeks. These bacteria are able to reduce levels of ammonium nitrogen, where the accumulation of nitrate nitrogen (NO3-) which is produced from the overhaul of nitrite nitrogen (NO2-) by nitrifying bacteria, is a signal that the maturation process of nitrifying bacteria has been achieved. The nitrification process consumes alkalis, hence lowers the pH value in the water, besides it also consumes a large amount of oxygen. The low number of bacteria and vibrio in the RAS system was affected by the use of a UV-sterilizer with a wavelength of 254 nm.
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Tisler, Witold, and Adam Szymkiewicz. "Air trapping problem during infiltration on the large areas." E3S Web of Conferences 44 (2018): 00178. http://dx.doi.org/10.1051/e3sconf/20184400178.
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The process of flow modeling in unsaturated porous medium is often found in many fields of sciences: geology, fluid mechanics, thermodynamics, microbiology or chemistry. Problem is relatively complicated due to complexity of the system which contains three phases: water, air and soil skeleton. The flow of water in such a medium can be described using two-phase (2PH) flow formulation, which accounts the inflow of air and water phases, or with simplified model known as Richards (RE) equation where only water flow is taken into account. In many well known programs available in the market (like SeepW, STOMP) the primary interest is only the water flow and the flow of air is omitted. As a result Richard equation in used more often. It’s main assumption is that pore air is continuous and has connection with atmospheric air which is equivalent to infinite mobility of the air phase during all simulation. This paper presents a brief review of the influence of the air phase in soil on water flow and pore pressure generation, with focus on applications related to infiltration process occurring in the large areas. An irrigation effect of rice fields with shallow water table has been investigated. To assess the impact of the gas phase various lengths of the infiltration zone have been considered. Numerical simulations are carried out to investigate the differences between the Richards equation and the two-phase flow model, using an in-house code based on the finite volume method.
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Keng, Zi Xiang, Jamie Jean Minn Tan, Bao Lee Phoon, Chee Chang Khoo, Ianatul Khoiroh, Siewhui Chong, Christinavimala Supramaniam, Ajit Singh, and Guan-Ting Pan. "Aerated Static Pile Composting for Industrial Biowastes: From Engineering to Microbiology." Bioengineering 10, no. 8 (August 7, 2023): 938. http://dx.doi.org/10.3390/bioengineering10080938.
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This work demonstrated the feasibility of an industrial-scale aerated static pile composting system for treating one of the common biowastes—soybean curd residue. The mixing ratios of the feedstock were optimized to achieve a carbon–nitrogen ratio and a moisture level in the ranges of 25–35 and 60–70%, respectively. This open-air composting system required 6–7 months to obtain a mature compost. Solvita and seed germination tests further confirmed the maturity of the compost, with 25% compost extract concentration yielding the best germination index in the absence of phytotoxicity. The bacterial and fungal compositions of the compost piles were further examined with metagenomic analysis. Thermoactinomyces spp., Oceanobacillus spp., and Kroppenstedtia spp. were among the unique bacteria found, and Diutina rugosa, Thermomyces dupontii, and Candida taylorii were among the unique fungi found in the compost piles, suggesting the presence of good microorganisms for degrading the organic biowastes.
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James, Greg. "PC4 laboratory construction: a users’ point of view." Microbiology Australia 29, no. 2 (2008): 86. http://dx.doi.org/10.1071/ma08086.
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The Sydney 2000 Olympic Games was a tremendous event for Australia, bringing the world?s best athletes and thousands of visitors to Sydney. As it was a global event with comprehensive media coverage, it also bought to our shores the possibility of terrorist activity. During the period the games were held, the Centre for Infectious Diseases and Microbiology (CIDM) performed laboratory investigations of suspicious substances found at Olympic Games venues and Sydney airport and worked with the Defence Science and Technology Organisation (DSTO) testing air samples, taken at venues, for the presence of bioterrorism agents.
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Soeldner, Al. "What Is It You Want From Us?" Microscopy Today 3, no. 8 (October 1995): 10–11. http://dx.doi.org/10.1017/s1551929500062891.
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I manage a university service electron microscope facility which provides transmission, scanning transmission, and scanning electron microscopy, each with X-ray energy spectrometry, as well as feature analysis, specimen preparation, and training services. Clients of the facility do basic and applied research in:human, animal, and plant health, pharmacy, and bioengineering;air, water, and soil ecology, pollution, and resource development and conservation;forest pathology, productivity, and development and performance of wood products;foods and nutrition:engineering, advanced metallic, ceramic, glass, and polymeric materials development, failure analysis, and tribology:botany, entomology, microbiology, zoology, chemistry, geology, and physics.
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I Putu Mas, Arie Pradina Putri. "Pemeriksaan Kualitas Air Minum Secara Mikrobiologis di Daerah Bukit Jimbaran." CERATA Jurnal Ilmu Farmasi 12, no. 2 (December 7, 2021): 1–7. http://dx.doi.org/10.61902/cerata.v12i2.200.
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The main problem faced in drinking water consumtion is contamination both chemically and biologically. The increasing population in the Bukit Jimbaran area makes demand for drinking water increased. This study was aims to examine the quality of drinking water microbiologically in Bukit Jimbaran area. Research had been carried out in the Laboratory of Microbiology and Virology, Faculty of Mathematics and Natural Science, Udayana University by taking samples from 3 drilled wells and 3 refill drinking water depots randomly. This research was experimental used Most Probable Number (MPN) test for the microbiological of the water that consist of presumptive test using Lactose Broth (LB), confirmative test using Brilliant Green Lactose Broth (BGLB), and complete test using endo jelly media. The result of presumptive test showed two samples of drilled well drinking water and three samples of refill drinking water were positive for Coliform. The result of confirmative test and complete test showed that three refill drinking water samples were positive for Coliform faecal bacteria and E. coli, which showed that the samples were not suitable for consumption