To see the other types of publications on this topic, follow the link: AFLP.
Journal articles on the topic 'AFLP'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'AFLP.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Hagen, Ferry, Maria-Teresa Illnait-Zaragozi, Karen H. Bartlett, Daniëlle Swinne, Erik Geertsen, Corné H. W. Klaassen, Teun Boekhout, and Jacques F. Meis. "In Vitro Antifungal Susceptibilities and Amplified Fragment Length Polymorphism Genotyping of a Worldwide Collection of 350 Clinical, Veterinary, and Environmental Cryptococcus gattii Isolates." Antimicrobial Agents and Chemotherapy 54, no. 12 (September 20, 2010): 5139–45. http://dx.doi.org/10.1128/aac.00746-10.
Full textAbstract:
ABSTRACT The in vitro susceptibilities of a worldwide collection of 350 Cryptococcus gattii isolates to seven antifungal drugs, including the new triazole isavuconazole, were tested. With amplified fragment length polymorphism (AFLP) fingerprinting, human, veterinary, and environmental C. gattii isolates were subdivided into seven AFLP genotypes, including the interspecies hybrids AFLP8 and AFLP9. The majority of clinical isolates (n = 215) comprised genotypes AFLP4 (n = 76) and AFLP6 (n = 103). The clinical AFLP6 isolates had significantly higher geometric mean MICs for flucytosine and fluconazole than the clinical AFLP4 isolates. Of the seven antifungal compounds examined in this study, isavuconazole had the lowest MIC90 (0.125 μg/ml) for all C. gattii isolates, followed by a 1 log2 dilution step increase (MIC90, 0.25 μg/ml) for itraconazole, voriconazole, and posaconazole. Amphotericin B had an acceptable MIC90 of 0.5 μg/ml, but fluconazole and flucytosine had relatively high MIC90s of 8 μg/ml.
2
Abdel-Hadi, Ahmed, Markus Schmidt-Heydt, Roberto Parra, Rolf Geisen, and Naresh Magan. "A systems approach to model the relationship between aflatoxin gene cluster expression, environmental factors, growth and toxin production by Aspergillus flavus." Journal of The Royal Society Interface 9, no. 69 (August 31, 2011): 757–67. http://dx.doi.org/10.1098/rsif.2011.0482.
Full textAbstract:
A microarray analysis was used to examine the effect of combinations of water activity ( a w , 0.995–0.90) and temperature (20–42°C) on the activation of aflatoxin biosynthetic genes (30 genes) in Aspergillus flavus grown on a conducive YES (20 g yeast extract, 150 g sucrose, 1 g MgSO 4 ·7H 2 O) medium. The relative expression of 10 key genes ( aflF , aflD , aflE , aflM , aflO , aflP , aflQ , aflX , aflR and aflS ) in the biosynthetic pathway was examined in relation to different environmental factors and phenotypic aflatoxin B 1 (AFB 1 ) production. These data, plus data on relative growth rates and AFB 1 production under different a w × temperature conditions were used to develop a mixed-growth-associated product formation model. The gene expression data were normalized and then used as a linear combination of the data for all 10 genes and combined with the physical model. This was used to relate gene expression to a w and temperature conditions to predict AFB 1 production. The relationship between the observed AFB 1 production provided a good linear regression fit to the predicted production based in the model. The model was then validated by examining datasets outside the model fitting conditions used (37°C, 40°C and different a w levels). The relationship between structural genes ( aflD , aflM ) in the biosynthetic pathway and the regulatory genes ( aflS , aflJ ) was examined in relation to a w and temperature by developing ternary diagrams of relative expression. These findings are important in developing a more integrated systems approach by combining gene expression, ecophysiological influences and growth data to predict mycotoxin production. This could help in developing a more targeted approach to develop prevention strategies to control such carcinogenic natural metabolites that are prevalent in many staple food products. The model could also be used to predict the impact of climate change on toxin production.
3
Mackill, D. J., Z. Zhang, E. D. Redoña, and P. M. Colowit. "Level of polymorphism and genetic mapping of AFLP markers in rice." Genome 39, no. 5 (October 1, 1996): 969–77. http://dx.doi.org/10.1139/g96-121.
Full textAbstract:
Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for gene mapping in plant species. We compared the levels of polymorphism for AFLP, RAPD, and microsatellite markers on 12 japonica and 2 indica rice cultivars. For AFLPs, seven EcoRI and seven MseI primers used in 18 primer combinations generated a total of 529 bands, of which 147 were clearly polymorphic among the accessions. The 21 RAPD primers produced 103 bands of which 43 were polymorphic. For the microsatellite markers the number of alleles per locus ranged from one (1 locus) to six. All marker types gave the same classification of the rice accessions into subspecies. Within japonica cultivars, the average percent polymorphism between any two accessions was 22% for AFLP, 24% for RAPD, and 36% for microsatellite markers (monomorphic bands excluded). The average percent polymorphism between indica and japonica accessions was 65, 35, and 76%, for AFLP, RAPD, and microsatellite markers, respectively. The total number of polymorphic bands was much higher for AFLPs, averaging over eight per gel. Seven AFLP primer combinations were assayed on 80 F2 plants of an indica × japonica cross previously mapped with RFLP markers. Of 54 AFLP bands scored, 50 could be mapped to specific chromosomes, and these appeared to be distributed throughout the rice genome. This indicates that AFLPs are a promising marker for mapping important genes in rice. Key words : Oryza sativa, AFLP, genetic mapping, polymorphism.
4
Ipek, Meryem, Ahmet Ipek, and Philipp W. Simon. "Comparison of AFLPs, RAPD Markers, and Isozymes for Diversity Assessment of Garlic and Detection of Putative Duplicates in Germplasm Collections." Journal of the American Society for Horticultural Science 128, no. 2 (March 2003): 246–52. http://dx.doi.org/10.21273/jashs.128.2.0246.
Full textAbstract:
Garlic (Allium sativum L.) is an asexually propagated crop that displays much morphological diversity. Studies which have assessed garlic diversity with isozymes and randomly amplified polymorphic DNA (RAPD) markers generally agreed with the morphological observations but sometimes failed to discriminate clones. To discriminate among closely related garlic clones in more detail, we introduced amplified fragment-length polymorphism (AFLPs) to evaluate the genetic diversity and phenetic relatedness of 45 garlic clones and three A. longicuspis clones and we compared AFLP results with RAPD markers and isozymes. Three AFLP primer combinations generated a total of 183 polymorphic fragments. Although similarities between the clusters were low (≥0.30), some clones within the clusters were very similar (>0.95) with AFLP analysis. Sixteen clones represented only six different banding patterns, within which they shared 100% polymorphic AFLPs and RAPD markers, and likely are duplicates. In agreement with the results of other investigators, A. longicuspis and A. sativum clones were clustered together with no clear separation, suggesting these species are not genetically or specifically distinct. The topology of AFLP, RAPD, and isozyme dendrograms were similar, but RAPD and isozyme dendrograms reflected less and much less polymorphism, respectively. Comparison of unweighted pair group method with arithmetic averaging (UPGMA) dendrograms of AFLP, RAPD, and isozyme cluster analyses using the Mantel test indicated a correlation of 0.96, 0.55, and 0.57 between AFLP and RAPD, AFLP and isozyme, and RAPD and isozyme, respectively. Polymorphic AFLPs are abundant in garlic and demonstrated genetic diversity among closely related clones which could not be differentiated with RAPD markers and isozymes. Therefore, AFLP is an additional tool for fingerprinting and detailed assessment of genetic relationships in garlic.
5
Liu, X. Z., C. S. He, Y. M. Yang, and H. Y. Zhang. "Genetic diversity among flue-cured tobacco cultivars on the basis of AFLP markers." Czech Journal of Genetics and Plant Breeding 45, No. 4 (December 27, 2009): 155–59. http://dx.doi.org/10.17221/15/2009-cjgpb.
Full textAbstract:
AFLP analyses were used to assess the genetic similarity among selected accessions at the South China Tobacco Breeding Research Centre (Yunnan province, Southwest China). 154 AFLP polymorphic fragments out of 561 fragments were used to assess the genetic diversity among 28 tobacco accessions. The average number of polymorphic bands per AFLP primer pair was 15.4. AFLPs seemed to be an effective classification tools for germplasm conservation and breeding. Limited genetic variation was detected within this group of accessions. The relationship of cultivars was estimated by cluster analysis based on AFLP data.
6
Albertini, Emidio, Andrea Porceddu, Gianpiero Marconi, Gianni Barcaccia, Luca Pallottini, and Mario Falcinelli. "Microsatellite-AFLP for genetic mapping of complex polyploids." Genome 46, no. 5 (October 1, 2003): 824–32. http://dx.doi.org/10.1139/g03-058.
Full textAbstract:
In spite of the economical relevance of polyploid crops, genetic mapping of these species has been relatively overlooked. This is because of intrinsic difficulties such as the uncertainty of the chromosome behavior at meiosis I and the need for very large segregating populations. An important, yet underestimated issue, in mapping polyploids is the choice of the molecular marker system. An ideal molecular marker system for polyploid mapping should maximize the percentage of single dose markers (SDMs) detected and the possibility of recognizing allelic markers. In the present work, the marker index for genetic mapping (MIgm) of M-AFLP is compared with that of AFLP and SAMPL. M-AFLPs have the highest MIgm values (22 vs. 18.5 of SAMPL and 9.83 of AFLP) mostly because of their high power to detect polymorphism. Owing to their prevalent codominant inheritance, it is proposed that M-AFLP can be used for the preliminary identification of hom(e)ologous groups.Key words: AFLP, mapping, microsatellite-AFLP, polyploids, SSR.
7
Evaristo, I., S. Santos, R. Tenreiro, and R. Costa. "Comparison of Genetic Structure Assessed by Amplified Fragment Length Polymorphism and Retrotransposon-based Sequence-specific Amplification Polymorphism for Portuguese Populations of Pinus pinea L." Silvae Genetica 57, no. 1-6 (December 1, 2008): 93–100. http://dx.doi.org/10.1515/sg-2008-0015.
Full textAbstract:
Abstract In order to assess genetic diversity within and among populations of Pinus pinea L. (stone pine), seven Portuguese populations originating from three Provenance Regions were selected and genotyped using two marker systems. We compared the genetic variation of these populations using retrotransposon-based sequence-specific amplification polymorphism (SSAP) and amplified fragment length polymorphism (AFLP). In total, 105 trees were screened with three primer enzyme combinations (PEC), producing 232 SSAP and 132 AFLP loci. Where SSAP yielded approximately twice-the number of polymorphic fragments compared to AFLP. Differentiation was slightly higher for SSAP, than for AFLP (FST = 0.105 for SSAP and 0.074 for AFLP), and both significantly different from zero, P < 0.01. The levels of average genetic diversity within-population found with the two types of marker were not significantly different between SSAPs and AFLPs (26.6% and 22.8%, respectively). The populations that displayed the highest and lowest genetic diversity scores were the same for both markers, and only two populations had significantly different He estimates. The neighbor-joining tree based on the Nei’s genetic distance displayed some geographic pattern. With the AFLP markers the populations grouped according to the provenance regions where they were sampled, resulting in one well supported cluster with the Southern populations, but with SSAP the pattern was not so coherent. In this study SSAP generated more polymorphic fragments and higher estimates of genetic diversity than AFPL did, due, probably, to the higher mutation rate of retrotransposition relative to base mutation. Nevertheless, congruence was found between estimates obtained with both markers, which is very interesting, for, in general, SSAP markers have lower costs compared to AFLPs, and they might be an interesting alternative marker system, when higher resolution is requested.
8
Posselt, U. K., P. Barre, G. Brazauskas, and L. B. Turner. "Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs." Czech Journal of Genetics and Plant Breeding 42, No. 3 (November 21, 2011): 87–94. http://dx.doi.org/10.17221/3647-cjgpb.
Full textAbstract:
Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92).
9
Aranzana, Maria José, Joaquim Carbó, and Pere Arús. "Using Amplified Fragment-length Polymorphisms (AFLPs) to Identify Peach Cultivars." Journal of the American Society for Horticultural Science 128, no. 5 (September 2003): 672–77. http://dx.doi.org/10.21273/jashs.128.5.0672.
Full textAbstract:
A sample of 210 cultivars of Prunus persica (L.) Batsch, with a wide range of fruit and plant characteristics, was studied for variability using nine polymorphic amplified fragment length polymorphism (AFLP) primer combinations. Forty-seven AFLPs allowed identification of 196 (93%) different genotypes, 187 of which could be distinguished with three primer combinations. Eleven cultivars with the same AFLP phenotype corresponded to known somatic mutations (sports), but from the four sports of the `Springcrest' group, two (`Maycrest' and `Queencrest') differed at three AFLPs from the others (`Starcrest' and `Early Maycrest'). Cluster analysis allowed differentiation of most cultivars with nonmelting fruit flesh, generally used for canning, from the melting-flesh peach and nectarine cultivars used for fresh consumption.
10
Hale, Anna, and Mark W. Farnham. "(5) A Comparative Study of SRAP, AFLP, and SSR Markers for Detecting Genetic Differences among Elite Broccoli Inbreds." HortScience 40, no. 4 (July 2005): 998E—999. http://dx.doi.org/10.21273/hortsci.40.4.998e.
Full textAbstract:
Private and public vegetable breeders are interested in using current and emerging PCR-based marker systems in their respective improvement programs. However, before new systems are employed to replace existing ones, the new systems must prove to be efficient and cost-effective alternatives. Sequence related amplified polymorphisms (SRAPs), amplified fragment length polymorphisms (AFLPs), and simple sequence repeats (SSRs) were compared for their ability to differentiate individuals of a diverse group of 24 elite broccoli (Brassica oleracea L. italica) inbreds. Genomic DNA was assayed using 24 AFLP, 24 SRAP, and 44 SSR primer pairs. In this assessment, SSRs produced an average of only two bands per primer, with 25% of these bands being monomorphic, and the remaining bands detecting very few differences among the inbreds. Although the AFLP method resulted in a lower rate (63%) of polymorphism than the SSRs, it produced about 20 bands per primer. SRAPs produced an average of 14 bands per primer, with 82% of these bands being polymorphic. Since AFLP and SRAP markers had a higher multiplex ratio and SSRs were frequently monomorphic, AFLP and SRAPs were more effective in differentiating the elite broccoli inbreds examined in this study. Similarity matrices were generated from the AFLP and SRAP data, and resulting dendographs were compared.
11
Hagidimitriou, Marianna, Andreas Katsiotis, George Menexes, Constantinos Pontikis, and Michael Loukas. "Genetic Diversity of Major Greek Olive Cultivars Using Molecular (AFLPs and RAPDs) Markers and Morphological Traits." Journal of the American Society for Horticultural Science 130, no. 2 (March 2005): 211–17. http://dx.doi.org/10.21273/jashs.130.2.211.
Full textAbstract:
The aim of the present study was to develop a reliable reference database to discriminate between the major Greek olive (Olea europaea L.) cultivars and reveal their genetic relationships, since Greece is considered a secondary center of diversity. In order to establish genetic relationships among the 26 Greek and eight international cultivars, four amplified fragment length polymorphism (AFLP) primer pairs, 12 randomly amplified polymorphic DNA (RAPD) primers, along with measurements from 10 morphological traits, were used. A total of 576 AFLP and 113 RAPD markers were produced. Genetic similarities, estimated using the Jaccard algorithim, ranged from 0.45 to 0.83 for the AFLP data and 0.27 to 0.87 for the RAPD data. The cophenetic correlation coefficients between the genetic similarities and the unweighted pair group method of arithmetic averages (UPGMA) phenograms were 0.77 for the AFLPs, 0.81 for the RAPDs, and 0.69 for the morphological traits. However, limited clustering similarities among the phenograms derived from the three methods were observed. This was also reflected by the low correlation between the three genetic similarity matrices produced (AFLP and RAPD, r = 0.39; AFLP and morphological traits, r = 0.11; RAPD and morphological traits, r = 0.02). According to the molecular results, olive cultivars are clustered according to fruit size but not according to geographical origin. Three of the cultivars tested, `Vasilicada,' `Throumbolia', and `Lianolia Kerkiras', were found to branch distantly to the others, according to the AFLP results, and can be considered as ancient Greek cultivars.
12
Xu, Mingliang, Ernesto Huaracha, and Schuyler S. Korban. "Development of sequence-characterized amplified regions (SCARs) from amplified fragment length polymorphism (AFLP) markers tightly linked to the Vf gene in apple." Genome 44, no. 1 (February 1, 2001): 63–70. http://dx.doi.org/10.1139/g00-103.
Full textAbstract:
Amplified fragment length polymorphism (AFLP) markers have become widely used in saturating the region of a gene of interest for the ultimate goal of map-based cloning of the gene or for marker-assisted selection. However, conversion of AFLP markers into restriction fragment length polymorphism (RFLP) or polymerase chain reaction (PCR)-based markers will greatly expand their usefulness in genetic applications. Previously, we have identified 15 AFLP markers tightly linked to the Vf gene conferring scab resistance in apple. In this study, we have successfully converted 11 of these AFLPs into sequence-characterized amplified region (SCAR) markers. Of the remaining four nonconverted AFLP markers, one, ET2MC8-1, has been found to be very short (83 base pairs) and is an A/T rich (90%) marker; a second, EA2MG11-1, has shown identical sequences between Malus floribunda 821 (the original source of the Vf gene) and scab-susceptible apple cultivars; while the other two, EA12MG16-1 and ET8MG1-1, have not been cloned. Using the 11 converted SCAR markers along with 5 previously identified SCAR markers, a high-resolution linkage map around the Vf gene has been constructed, and found to be consistent with its corresponding AFLP map. Three converted SCAR markers (ACS-3, -7, and -9) are inseparable from the Vf gene; whereas one (ACS-6) is located left of, and the remaining seven (ACS-1, -2, -4, -5, -8, -10, and -11) are located right of the Vf gene at genetic distances of 0.4 and 0.2 cM, respectively. A reliable and robust procedure for development of SCAR markers from AFLP markers is presented.Key words: apple, AFLP, SCAR, apple scab disease, Vf gene.
13
Wong, A., M. R. Forbes, and M. L. Smith. "Characterization of AFLP markers in damselflies: prevalence of codominant markers and implications for population genetic applications." Genome 44, no. 4 (August 1, 2001): 677–84. http://dx.doi.org/10.1139/g01-051.
Full textAbstract:
Amplified fragment length polymorphism (AFLP) analysis is becoming increasingly popular as a method for generating molecular markers for population genetic applications. For practical considerations, it is generally assumed in population studies that AFLPs segregate as dominant markers, i.e., that present and absent are the only possible states of a given locus. We tested the assumption of dominance in natural populations of the damselfly Nehalennia irene (Hagen) (Odonata: Coenagrionidae). Electro-blotted AFLP products from 21 samples were probed with individual markers. Eleven markers were analyzed, of which two were monomorphic and nine were polymorphic. Only two of the polymorphic markers behaved in a strictly dominant manner. The remaining seven polymorphic markers displayed various degrees of codominance, with 210 visible alleles in the sample. Of the three markers displaying the highest degree of variability, two contained microsatellite repeat tracts. Our results suggest that the assumption of dominance is unfounded. As a result, AFLP analysis may be unsuitable for estimating several important population genetic parameters, including genetic diversity.Key words: AFLP, population genetics, dominant markers, microsatellite, insect, damselfly.
14
Bradeen, James M., and Philipp W. Simon. "AFLP-derived, Codominant Markers for Locus-specific Applications." HortScience 33, no. 3 (June 1998): 514e—514. http://dx.doi.org/10.21273/hortsci.33.3.514e.
Full textAbstract:
The amplified fragment length polymorphism (AFLP) is a powerful marker, allowing rapid and simultaneous evaluation of multiple potentially polymorphic sites. Although well-adapted to linkage mapping and diversity assessment, AFLPs are primarily dominant in nature. Dominance, relatively high cost, and technological difficulty limit use of AFLPs for marker-aided selection and other locus-specific applications. In carrot the Y2 locus conditions carotene accumulation in the root xylem. We identified AFLP fragments linked to the dominant Y2 allele and pursued conversion of those fragments to codominant, PCR-based forms useful for locus-specific applications. The short length of AFLPs (≈60 to 500 bp) precludes development of longer, more specific primers as in SCAR development. Instead, using sequence information from cloned AFLP fragments for primer design, regions outside of the original fragment were amplified by inverse PCR or ligation-mediated PCR, cloned, and sequenced. Differences in sequences associated with Y2 vs. y2 allowed development of simple PCR assays differentiating those alleles. PCR primers flanking an insertion associated with the recessive allele amplified differently sized products for the two Y2 alleles in one assay. This assay is rapid, technologically simple (requiring no radioactivity and little advanced training or equipment), reliable, inexpensive, and codominant. Our PCR assay has a variety of large scale, locus-specific applications including genotyping diverse carrot cultivars and wild and feral populations. Efforts are underway to improve upon conversion technology and to more extensively test the techniques we have developed.
15
Salamini, F., M. Heun, A. Brandolini, H. Özkan, and J. Wunder. "Comment on "AFLP data and the origins of domesticated crops"." Genome 47, no. 3 (June 1, 2004): 615–20. http://dx.doi.org/10.1139/g04-013.
Full textAbstract:
We review some concepts and methods of handling and using DNA fingerprinting in phylogenetic analyses related to crop domestication. Particular reference is made to AFLP markers and mode and place of einkorn, barley, and tetraploid wheat domestication in the Neolithic by human communities in the Fertile Crescent. The reconsideration of AFLP databases of domesticated and wild lines demonstrates that phylogenetic tree topologies, originally described for the three species, match closely the new results obtained by principle coordinate analyse.Key words: AFLPs, discontinuous markers, crop domestication, einkorn wheat, barley, tetraploid wheat.
16
Park, Young Hoon, Marilyn A. L. West, and Dina A. St. Clair. "Evaluation of AFLPs for germplasm fingerprinting and assessment of genetic diversity in cultivars of tomato (Lycopersicon esculentum L.)." Genome 47, no. 3 (June 1, 2004): 510–18. http://dx.doi.org/10.1139/g04-004.
Full textAbstract:
Cultivated tomato (L. esculentum L.) germplasm exhibits limited genetic variation compared with wild Lycopersicon species. Amplified fragment length polymorphism (AFLP) markers were used to evaluate genetic variation among 74 cultivars, primarily from California, and to fingerprint germplasm to determine if cultivar-specific patterns could be obtained. All 74 cultivars were genotyped using 26 AFLP primer combinations; of the 1092 bands scored, 102 AFLP bands (9.3%) were polymorphic. Pair-wise genetic similarity coefficients (Jaccard and Nei–Li) were calculated. Jaccard coefficients varied from 0.16 to 0.98 among cultivar pairs, and 72% of pair-wise comparisons exceeded 0.5. UPGMA (unweighted pair-group method with arithmetic averaging) clustering and principle component analysis revealed four main clusters, I–IV; most modern hybrid cultivars grouped in II, whereas most vintage cultivars grouped in I. Clusters III and IV contained three and two cultivars, respectively. Some groups of cultivars closely related by pedigree exhibited high bootstrap values, but lower values (<50%) were obtained for cluster II and its four subgroups. Unique fingerprints for all 74 cultivars were obtained by a minimum of seven AFLP primer pairs, despite inclusion of some closely related cultivars. This study demonstrated that AFLP markers are effective for obtaining unique fingerprints of, and assessing genetic diversity among, tomato cultivars.Key words: Lycopersicon esculentum, AFLPs, DNA fingerprinting, genetic diversity, phenetic relationships.
17
He, Congfen, Badraldin Ebrahim Sayed-Tabatabaei, and Takao Komatsuda. "AFLP targeting of the 1-cM region conferring the vrs1 gene for six-rowed spike in barley, Hordeum vulgare L." Genome 47, no. 6 (December 1, 2004): 1122–29. http://dx.doi.org/10.1139/g04-073.
Full textAbstract:
Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well-characterized near-isogenic lines as plant materials. The AFLP markers were integrated into three different maps, in which 'Azumamugi' was used as the maternal parent. Of the three maps, Hordeum vulgare L. 'Azumamugi' × H. vulgare 'Golden Promise' showed recombination of the AFLP markers and the vrs1 locus (closest, 0.05 cM), providing the best mapping population for positional cloning of alleles at the vrs1 locus. Conversion of AFLP bands into polymorphic sequence-tagged sites (STSs) is necessary for further high-throughput genotype scoring and for bacterial artificial chromosome (BAC) library screening. We cloned and sequenced the five AFLP bands and synthesized primer pairs. PCR amplification generated DNAs of the same size from all four parental lines for each marker. Restriction endonuclease treatment of e40m36-1110/AccIII, e34m13-260/Psp1406I, e52m32-270/FokI, and e31m26-520/MnlI revealed fragment length polymorphisms between 'Azumamugi' and all the two-rowed parents. Allelism between the AFLPs and corresponding STS markers was confirmed genetically, indicating the usefulness of the STSs as genetic markers.Key words: positional cloning, codominance, near-isogenic lines, high-resolution maps, STSs.
18
Clark, Rebecca, Sarah M. Brown, Steve C. Collins, Chris D. Jiggins, David G. Heckel, and Alfried P. Vogler. "Colour pattern specification in the Mocker swallowtail Papilio dardanus : the transcription factor invected is a candidate for the mimicry locus H." Proceedings of the Royal Society B: Biological Sciences 275, no. 1639 (February 19, 2008): 1181–88. http://dx.doi.org/10.1098/rspb.2007.1762.
Full textAbstract:
The swallowtail butterfly, Papilio dardanus , is an iconic example of a polymorphic Batesian mimic. The expression of various female-limited colour forms is thought to be controlled by a single autosomal locus, termed H , whose function in determining the wing pattern remains elusive. As a step towards the physical mapping of H , we established a set of 272 polymorphic amplified fragment length polymorphism (AFLP) markers ( Eco RI- Mse I). Segregation patterns in a ‘female-informative’ brood (exploiting the absence of crossing over in female Lepidoptera) mapped these AFLPs to 30 linkage groups (putative chromosomes). The difference between the hippocoon and cenea female forms segregating in this family resides on a single one of these linkage groups, defined by 14 AFLPs. In a ‘male-informative’ cross (markers segregating within a linkage group), a pair of AFLPs co-segregated closely with the two female forms, except in four recombinants out of 19 female offspring. Linkage with these AFLP markers using four further female-informative families demonstrated that the genetic factor determining other morphs ( poultoni , lamborni and trimeni ) also maps to this same linkage group. The candidate gene invected , obtained in a screen for co-segregation of developmental genes with the colour forms, resides in a 13.9 cM interval flanked by the two AFLP markers. In the male-informative family invected co-segregated perfectly with the hippocoon / cenea factor, despite the four crossovers with the AFLPs. These findings make invected , and possibly its closely linked paralogue engrailed , strong candidates for H . This is supported by their known role in eyespot specification in nymphalid butterfly wings.
19
Brubaker, Curt L., and Anthony H. D. Brown. "The use of multiple alien chromosome addition aneuploids facilitates genetic linkage mapping of the Gossypium G genome." Genome 46, no. 5 (October 1, 2003): 774–91. http://dx.doi.org/10.1139/g03-063.
Full textAbstract:
Primary germplasm pools represent the most accessible source of new alleles for crop improvement, but not all effective alleles are available in the primary germplasm pool, and breeders must sometimes confront the difficulties of introgressing genes from the secondary and tertiary germplasm pools in cotton by using synthetic polyploids as introgression bridges. Two parental Gossypium nelsonii × Gossypium australe AFLP genetic linkage maps were used to identify G genome chromosome-specific molecular markers, which in turn were used to track the fidelity and frequency of G. australe chromosome transmission in a Gossypium hirsutum × G. australe hexaploid bridging family. Conversely, when homoeologous recombination is low, first generation aneuploids are useful adjuncts to genetic linkage mapping. Although locus ordering was not possible, the distribution of AFLP markers among 18 multiple chromosome addition aneuploids identified mapping errors among the G. australe and G. nelsonii linkage groups and assigned non-segregating G. australe AFLPs to linkage groups. Four putatively recombined G. australe chromosomes were identified in 5 of the 18 aneuploids. The G. australe and G. nelsonii genetic linkage maps presented here represent the first AFLP genetic linkage maps for the Gossypium G genome.Key words: Gossypium, G genome, AFLP, cotton, aneuploid.
20
Levi, Amnon, Claude E. Thomas, M. Newman, O. U. K. Reddy, X. Zhang, and Y. Xu. "ISSR and AFLP Markers Differ among American Watermelon Cultivars with Limited Genetic Diversity." Journal of the American Society for Horticultural Science 129, no. 4 (July 2004): 553–58. http://dx.doi.org/10.21273/jashs.129.4.0553.
Full textAbstract:
Wide phenotypic diversity exists among American heirloom cultivars of watermelon (Citrullus lanatus var. lanatus). However, in published studies, low or no polymorphism was revealed among those heirlooms using isozyme or randomly amplified polymorphic DNA (RAPD) markers. In this study, experiments with inter-simple sequence repeat (ISSR) [also known as simple sequence repeat-(SSR-) anchored primers] and amplified fragment-length polymorphism (AFLP) markers produced high polymorphisms among watermelon heirloom cultivars. ISSR (111) and AFLP (118) markers (229 total) identified 80.2% to 97.8% genetic similarity among heirloom cultivars. The phylogenetic relations based on ISSR and AFLP markers are highly consistent with the parental records available for some of the heirloom cultivars, providing confidence in the dendogram constructed for heirlooms based on similarity values. As compared with RAPD markers, ISSRs and AFLPs are highly effective in differentiating among watermelon cultivars or elite lines with limited genetic diversity.
21
NEMATI, Zahra, Ali TEHRANIFAR, Mohammad FARSI, Amin MIRSHAMSI KAKHKI, Hossein NEMATI, and Mehdi KHAYAT. "Evaluation of Genetic Diversity of Iranian Pomegranate Cultivars Using Fruit Morphological Characteristics and AFLP Markers." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 40, no. 1 (May 14, 2012): 261. http://dx.doi.org/10.15835/nbha4017369.
Full textAbstract:
The present research evaluated the diversity of a number of Iranian pomegranate cultivars using fruit morphological characteristics and AFLP markers. Thirty-one pomegranate cultivars were collected from Yazd Pomegranate Collection in Iran to study their diversity. Seven AFLP primer combinations were used to amplify a total of 112 polymorphic fragments (47.26%). By use of AFLPs, a low genetic diversity level was detected among cultivars. The relationship between fruit characteristics was analyzed using the principal component analysis (PCA). The cluster analysis based on both fruit characteristics and AFLP data indicated that cultivars were not grouped according to their geographic origins. Moreover, the correlation between the diversity matrix based on fruit characteristics and Dice’s genetic similarity coefficient was insignificant (r=0.06). The results obtained from this study can improve the conservation and management of pomegranate germplasm resources and could be helpful in optimizing breeding programs.
22
Gedil, Melaku Ayele, Crispin Wye, Simon Berry, Bart Segers, Johan Peleman, Richard Jones, Alberto Leon, Mary B. Slabaugh, and Steven J. Knapp. "An integrated restriction fragment length polymorphism - amplified fragment length polymorphism linkage map for cultivated sunflower." Genome 44, no. 2 (April 1, 2001): 213–21. http://dx.doi.org/10.1139/g00-111.
Full textAbstract:
Restriction fragment length polymorphism (RFLP) maps have been constructed for cultivated sunflower (Helianthus annuus L.) using three independent sets of RFLP probes. The aim of this research was to integrate RFLP markers from two sets with RFLP markers for resistance gene candidate (RGC) and amplified fragment length polymorphism (AFLP) markers. Genomic DNA samples of HA370 and HA372, the parents of the F2 population used to build the map, were screened for AFLPs using 42 primer combinations and RFLPs using 136 cDNA probes (RFLP analyses were performed on DNA digested with EcoRI, HindIII, EcoRV, or DraI). The AFLP primers produced 446 polymorphic and 1101 monomorphic bands between HA370 and HA372. The integrated map was built by genotyping 296 AFLP and 104 RFLP markers on 180 HA370 × HA372 F2 progeny (the AFLP marker assays were performed using 18 primer combinations). The HA370 × HA372 map comprised 17 linkage groups, presumably corresponding to the 17 haploid chromosomes of sunflower, had a mean density of 3.3 cM, and was 1326 cM long. Six RGC RFLP loci were polymorphic and mapped to three linkage groups (LG8, LG13, and LG15). AFLP markers were densely clustered on several linkage groups, and presumably reside in centromeric regions where recombination is reduced and the ratio of genetic to physical distance is low. Strategies for targeting markers to euchromatic DNA need to be tested in sunflower. The HA370 × HA372 map integrated 14 of 17 linkage groups from two independent RFLP maps. Three linkage groups were devoid of RFLP markers from one of the two maps.Key words: amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), Helianthus, sunflower, genetic map.
23
Safari, Nassim, Mehran Mirabzadeh Ardakani, Roghayeh Hemmati, Alessia Parroni, Marzia Beccaccioli, and Massimo Reverberi. "The Potential of Plant-Based Bioactive Compounds on Inhibition of Aflatoxin B1 Biosynthesis and Down-regulation of aflR, aflM and aflP Genes." Antibiotics 9, no. 11 (October 23, 2020): 728. http://dx.doi.org/10.3390/antibiotics9110728.
Full textAbstract:
The use of plant extracts in pre- and post-harvest disease management of agricultural crops to cope with aflatoxin B1 contamination has shown great promise due to their capability in managing toxins and safe-keeping the quality. We investigated the anti-aflatoxigenic effect of multiple doses of eight plant extracts (Heracleum persicum, Peganum harmala, Crocus sativus, Trachyspermum ammi, Rosmarinus officinalis, Anethum graveolens, Berberis vulgaris, Berberis thunbergii) on Aspergillus flavus via LC-MS and the down-regulatory effect of them on aflR, aflM and aflP genes involved in the aflatoxin B1 biosynthesis pathway using RT-qPCR analyses. Our results showed that H. persicum (4 mg/mL), P. harmala (6 mg/mL) and T. ammi (2 mg/mL) completely stopped the production of aflatoxin B1, without inducing significant changes in A. flavus growth. Furthermore, our findings showed a highly significant correlation between the gene expression and the aflatoxin B1 biosynthesis, such that certain doses of the extracts reduced or blocked the expression of the aflR, aflM and aflP and consequently reduced the synthesis of aflatoxin B1. Interestingly, compared to the regulatory gene (aflR), the down-regulation of expression in the structural genes (aflM and aflP) was more consistent and correlated with the inhibition of aflatoxin B1 production. Overall, this study reveals the anti-aflatoxigenic mechanisms of the selected plant extracts at the gene expression level and provides evidence for their use in plant and crop protection.
24
Labombarda, Paola, Alessandra Busti, Maria Eugenia Caceres, Fulvio Pupilli, and Sergio Arcioni. "An AFLP marker tightly linked to apomixis reveals hemizygosity in a portion of the apomixis-controlling locus in Paspalum simplex." Genome 45, no. 3 (June 1, 2002): 513–19. http://dx.doi.org/10.1139/g02-014.
Full textAbstract:
A mapping population of Paspalum simplex segregating for apomixis (asexual reproduction through seeds) was screened with AFLPs to find apomixis-linked markers. Four AFLPs linked to apomixis in coupling phase were found. Three of them did not show recombinants among the 87 individuals of the mapping population, whereas the other was more loosely linked. Integrating the AFLP data with those obtained previously with rice RFLP anchor markers, a map was drawn for the chromosome region of P. simplex encompassing apomixis. We cloned the three AFLPs tightly linked with apomixis into plasmid vectors and used them as probes to hybridize the restriction digested DNA of the mapping population. Two of them revealed RFLP bands linked to apomixis together with other alleles, whereas one was proven to belong to a hemizygous portion of the apomixis locus. The total picture resulting from AFLP and RFLP analyses was that a cluster of markers tightly linked with apomixis was detected in P. simplex together with two other markers that were more loosely linked. These two markers enclosed a relatively large chromosome segment characterized by strong repression of recombination. The block of recombination may have caused sequence divergence and, therefore, hemizygosity of some regions belonging to the apomixis-controlling chromosome segment of P. simplex. The potential of developing an apomixis-specific sequence for screening large-fragment libraries for the physical isolation of the locus encompassing apomixis is discussed.Key-words: AFLP, apomixis, hemizygosity.
25
van Vugt, Joke J. F. A., Ron G. M. van der Hulst, Andrea J. P. Pruijssers, Patrick Verbaarschot, Richard Stouthamer, and Hans de Jong. "Comparative AFLP reveals paternal sex ratio chromosome specific DNA sequences in the parasitoid wasp Trichogramma kaykai." Genome 52, no. 5 (May 2009): 447–55. http://dx.doi.org/10.1139/g09-024.
Full textAbstract:
The parasitoid wasp Trichogramma kaykai with a haplo-diploid sex determination has a B chromosome called the paternal sex ratio (PSR) chromosome that confers paternal genome loss during early embryogenesis, resulting in male offspring. So far, it is not well known whether the PSR chromosome has unique DNA sequence characteristics. By comparative AFLP fingerprinting of genomic DNA from wasps with and without the PSR chromosome, we isolated DNA from PSR-specific bands. Fourteen of such DNA fragments were analysed to confirm their PSR specificity. Seven were sequenced and two (PT-AFLP 1 and PT-AFLP1 3) were identified as parts of retrotransposon genes based on BLAST searches. Internal primers designed from a third AFLP fragment allowed PCR amplification of a PSR chromosome specific marker, which can be used to screen for the PSR trait in male wasps. Southern analysis revealed a dispersed repetitive nature of this third sequence in the T. kaykai genome, suggesting that it is part of a transposon. A fourth AFLP fragment (PT-AFLP 5) appears to be a large repetitive sequence on the PSR chromosome. This sequence is also found in the genome of both T. kaykai and the closely related species Trichogramma deion , but its distribution on the PSR chromosome strongly resembles that of T. deion rather than that of T. kaykai. Our results provide further insight into the repetitive nature of sequences comprising B chromosomes and their similarities with their host and closely related species.
26
Zhou, Lili, Frank Kappel, Cheryl Hampson, Paul A. Wiersma, and Guus Bakkeren. "Genetic Analysis and Discrimination of Sweet Cherry Cultivars and Selections Using Amplified Fragment Length Polymorphism Fingerprints." Journal of the American Society for Horticultural Science 127, no. 5 (September 2002): 786–92. http://dx.doi.org/10.21273/jashs.127.5.786.
Full textAbstract:
Amplified fragment length polymorphisms (AFLPs) were used to analyze the relationships between sweet cherry (Prunus avium L.) cultivars and selections from the breeding program at the Pacific Agri-Food Research Centre in Summerland, Canada. Six pairs of preselected primers were used for the analysis of a total of 67 cultivars and selections. Scoring the absence and presence of 118 polymorphic DNA fragments produced a unique binary code for each cultivar and selection. Two phylogenetic trees were constructed using these 118 polymorphic fragments, one tree for 55 related cultivars and selections from the Summerland breeding program and the other for 23 self-incompatible cultivars of differing origins. The reliability of AFLP DNA fingerprints was confirmed by correlating relationships revealed by AFLP profiles with known genetic relationships of some sweet cherry cultivars and by a blind test for cultivar identification. Results indicate that AFLP analysis is a good technique to evaluate genetic distance and relationships in a sweet cherry breeding population.
27
Campbell, A. W., G. Daggard, F. Békés, A. Pedler, M. W. Sutherland, and R. Appels. "Targetting AFLP-DNA markers to specific traits and chromosome regions." Australian Journal of Agricultural Research 52, no. 12 (2001): 1153. http://dx.doi.org/10.1071/ar01028.
Full textAbstract:
The amplified fragment length polymorphism (AFLP) technique is widely used in mapping of wheat as high polymorphism rates are obtained and the procedure is relatively simple. In this study the AFLP markers were targetted to wheat chromosome regions of interest, especially those in which random mapping approaches located relatively few markers. Results showed that the combination of bulk segregant analysis and AFLP markers could target new markers to regions of interest in wheat, and as a result, additional markers were identified for the dough mixing time and noodle colour traits. The new markers for noodle colour were closer to this trait than the markers previously identified by random procedures. As a result, their association with the trait was more significant, an aspect that is important for selection efficiency. In order to improve genome coverage, it was found that regions of chromosomes containing telomere sequences could be targetted using AFLPs combined with a telomere sequence anchor primer.
28
Hale, Anna L., Mark W. Farnham, and Monica A. Menz. "Use of PCR-based Markers for Differentiating Elite Broccoli Inbreds." Journal of the American Society for Horticultural Science 131, no. 3 (May 2006): 418–23. http://dx.doi.org/10.21273/jashs.131.3.418.
Full textAbstract:
Breeders of cole crops (Brassica oleracea L.) have an interest in utilizing current and emerging PCR-based marker systems to differentiate elite germplasm. However, until efficiency and cost-effectiveness are determined, most breeders are hesitant to change methods. In this study, our goal was to compare simple sequence repeat (SSR), amplified fragment-length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) marker systems for their effectiveness in differentiating a diverse population of 24 elite broccoli (B. oleracea Italica Group) inbreds. Published SSR primer sequences for Brassica L. species were used along with AFLP and SRAP primer combinations. Several SSR primers failed to amplify DNA in the broccoli population, but all AFLP and SRAP primer combinations produced multiple bands. Twenty-nine percent of the SSR primers were monomorphic, while most of the remaining primers detected only one or two differences among inbreds. AFLP and SRAP methods produced multiple differences per primer in almost every case. Phenetic analysis revealed that the type of marker affected the classification of the genotypes. All three marker systems were able to successfully differentiate between the 24 elite inbreds, however, AFLPs and SRAPs were more efficient, making them better alternatives than SSRs over other established methods for fingerprinting B. oleracea inbreds.
29
Semagn, Kassa, Åsmund Bjørnstad, Helge Skinnes, Anne Guri Marøy, Yalew Tarkegne, and Manilal William. "Distribution of DArT, AFLP, and SSR markers in a genetic linkage map of a doubled-haploid hexaploid wheat population." Genome 49, no. 5 (May 1, 2006): 545–55. http://dx.doi.org/10.1139/g06-002.
Full textAbstract:
A genetic linkage mapping study was conducted in 93 doubled-haploid lines derived from a cross between Triticum aestivum L. em. Thell 'Arina' and a Norwegian spring wheat breeding line, NK93604, using diversity arrays technology (DArT), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) markers. The objective of this study was to understand the distribution, redundancy, and segregation distortion of DArT markers in comparison with AFLP and SSR markers. The map contains a total of 624 markers with 189 DArTs, 165 AFLPs and 270 SSRs, and spans 2595.5 cM. All 3 marker types showed significant (p < 0.01) segregation distortion, but it was higher for AFLPs (24.2%) and SSRs (22.6%) than for DArTs (13.8%). The overall segregation distortion was 20.4%. DArTs showed the highest frequency of clustering (27.0%) at < 0.5 cM intervals between consecutive markers, which is 3 and 15 times higher than SSRs (8.9%) and AFLPs (1.8%), respectively. This high proportion of clustering of DArT markers may be indicative of gene-rich regions and (or) the result of inclusion of redundant clones in the genomic representations, which was supported by the presence of very high correlation coefficients (r > 0.98) and multicollinearity among the clustered markers. The present study is the first to compare the utility of DArT with AFLP and SSR markers, and the present map has been successfully used to identify novel QTLs for resistance to Fusarium head blight and powdery mildew and for anther extrusion, leaf segment incubation, and latency.Key words: 'Arina', diversity arrays technology, double haploid, genetic map, marker clustering, microsatellite.
30
Allaby, Robin G., and Terence A. Brown. "AFLP data and the origins of domesticated crops." Genome 46, no. 3 (June 1, 2003): 448–53. http://dx.doi.org/10.1139/g03-025.
Full textAbstract:
Amplified fragment length polymorphism (AFLP) datasets have been used to construct neighbor-joining trees from which monophyletic origins for crops such as einkorn wheat, barley, and emmer wheat have been inferred. We simulated several different multiple domestication scenarios for an imaginary cereal crop and examined the resulting domesticated populations. The simulations showed that the population biology aspects of the domestication process can result in independently domesticated populations merging in such a way that a monophyletic origin is erroneously inferred when the resulting population is examined by AFLP genotyping and neighbor-joining analysis. The results bring into question the use of this method to infer the origins of real crops.Key words: AFLPs, agriculture, neighbor-joining, plant domestication.
31
Blas, Andrea L., Qingyi Yu, Cuixia Chen, Olivia Veatch, Paul H. Moore, Robert E. Paull, and Ray Ming. "Enrichment of a papaya high-density genetic map with AFLP markers." Genome 52, no. 8 (August 2009): 716–25. http://dx.doi.org/10.1139/g09-043.
Full textAbstract:
A high-density genetic linkage map of papaya, previously developed using an F2 mapping population derived from the intraspecific cross AU9 × SunUp, was enriched with AFLP markers. The comprehensive genetic map presented here spans 945.2 cM and covers 9 major and 5 minor linkage groups containing 712 SSR, 277 AFLP, and 1 morphological markers. The average marker density for the 9 major linkage groups is 0.9 cM between adjacent markers, and the total number of gaps >5 cM was reduced from 48 to 27 in the current map. AFLPs generated by EcoRI/MseI primer combinations were distributed throughout the 14 linkage groups and resulted in several large locus order rearrangements within the 9 major linkage groups. Integration of AFLP markers provided tighter linkage association between loci, leading to a reduction in map distance on LGs 1, 2, and 4, which were inflated in the previous map, and correction of the marker order on LG8. Suppression of recombination in the male-specific Y region (MSY) of LG1 is further validated by the addition of 27 sex co-segregating AFLP markers. A large region of distorted segregation surrounding the MSY spans 54.4 cM and represents ∼71% of the linkage group. This comprehensive high-density genetic map provides a framework for mapping quantitative trait loci and for fine mapping as well as for comparative genomic studies of crop plant development and evolution.
32
Markussen, T., A. Tusch, B. R. Stephan, and M. Fladung. "Identification of Molecular Markers for Selected Wood Properties of Norway Spruce Picea abies L. (Karst.) II. Extractives Content." Silvae Genetica 54, no. 1-6 (December 1, 2005): 145–52. http://dx.doi.org/10.1515/sg-2005-0022.
Full textAbstract:
Abstract We describe the development of a SCAR-marker linked to low extractives content of Norway Spruce (Picea abies L [Karst.]) derived from AFLPs. In these analyses 57 different primer enzyme combinations were used in a bulked segregant analysis approach comparing individuals with high and low extractives content. A total of 14 polymorphic AFLP markers were detected between the pools. Five markers were selected for further analyses to verify their linkage to extractives content based on individuals used for pool constitution. One AFLP marker, found to be significant linked to low extractives content was converted into a SCAR marker for further validation. For this marker, a monomorphic band was obtained by using sets of nested primers or restriction site specific primers (RSS) which include the AFLP-restriction recognition site. The separation of the marker from unlinked size homologous marker-alleles was realized by a SSCP-approach. Validation of the marker on different full-sib families confirmed the usability to separate the classes for low and high extractives content of Picea abies.
33
Krauss, Siegfried L., and Rod Peakall. "An Evaluation of the AFLP Fingerprinting Technique for the Analysis of Paternity in Natural Populations of Persoonia mollis (Proteaceae)." Australian Journal of Botany 46, no. 4 (1998): 533. http://dx.doi.org/10.1071/bt97043.
Full textAbstract:
The accurate assignment of paternity in natural plant populations is required to address important issues in evolutionary biology, such as the factors that affect reproductive success. Newly developed molecular fingerprinting techniques offer the potential to address these aims. Here, we evaluate the utility of a new PCR-based multi-locus fingerprinting technique called Amplified Fragment Length Polymorphism (AFLP) for paternity studies in Persoonia mollis (Proteaceae). AFLPs were initially scored for five individuals from three taxonomic levels for 64 primer pairs: between species (P. mollis and P. levis), between subspecies (P. mollis subsp. nectens and subsp. livens), between individuals within a single population of P. mollis, as well as for a naturally pollinated seed from a single P. mollis subsp. nectens plant. Overall, 1164 fragments (24.6% of all fragments) were polymorphic between species, 743 (16.5%) between subspecies, 371 (8.6%) between individuals within a single population, and 265 (6.2%) between a plant and its seed. Within a single P. mollis population of 14 plants, 42 polymorphic fragments were scored from profiles generated by a single AFLP primer pair. The mean frequency of the recessive allele (q) over these 42 loci was 0.773. Based on these observations, it will be feasible to generate well over 100 polymorphic AFLP loci with as few as three AFLP primer pairs. This level of polymorphism is sufficient to assign paternity unambiguously to more than 99% of all seed in experiments involving small, known paternity pools. More generally, the AFLP procedure is well suited to molecular ecological studies, because it produces more polymorphism than allozymes or RAPDs but, unlike conventionally developed microsatellite loci, it requires no prior sequence knowledge and minimal development time.
34
Lu, Yingzhi, Jessica Curtiss, Danielle Miranda, Ed Hughs, and Jinfa Zhang. "ATG-anchored AFLP (ATG-AFLP) analysis in cotton." Plant Cell Reports 27, no. 10 (June 27, 2008): 1645–53. http://dx.doi.org/10.1007/s00299-008-0568-z.
Full text
35
Miyashita, Naohiko T., Akira Kawabe, and Hideki Innan. "DNA Variation in the Wild Plant Arabidopsis thaliana Revealed by Amplified Fragment Length Polymorphism Analysis." Genetics 152, no. 4 (August 1, 1999): 1723–31. http://dx.doi.org/10.1093/genetics/152.4.1723.
Full textAbstract:
Abstract To investigate the level and pattern of DNA variation of Arabidopsis thaliana at the entire genome level, AFLP analysis was conducted for 38 ecotypes distributed throughout the world. Ten pairs of selective primers were used to detect a total of 472 bands, of which 374 (79.2%) were polymorphic. The frequency distribution of polymorphic bands was skewed toward an excess of singleton variation. On the basis of AFLP variation, nucleotide diversity for the entire genome was estimated to be 0.0106, which was within the range reported previously for specific nuclear genes. The frequency distribution of pairwise distance was bimodal because of an ecotype (Fl-3) with a large number of unique bands. Linkage disequilibrium between polymorphic AFLPs was tested. The proportion of significant linkage disequilibria was close to random expectation after neglecting the ecotype Fl-3. This result indicates that the effect of recombination could not be ignored in this selfing species. A neighbor-joining tree was constructed on the basis of the AFLP variation. This tree has a star-like topology and shows no clear association between ecotype and geographic origin, suggesting a recent spread of this plant species and limited migration between its habitats.
36
Zhang, Hangning, Shuhei Nasuda, and Takashi R. Endo. "Identification of AFLP markers on the satellite region of chromosome 1BS in wheat." Genome 43, no. 5 (October 1, 2000): 729–35. http://dx.doi.org/10.1139/g00-039.
Full textAbstract:
The satellite region on the short arm of chromosome 1B in wheat (Triticum aestivum L., 2n = 6x = 42) carries many agronomically important genes; i.e., genes conferring fungal disease resistance, seed storage proteins, and fertility restoration. To find molecular markers located on the satellite region, we applied the fluorescent AFLP (amplified fragment length polymorphism) technique to aneuploids and deletion stocks of the cultivar T. aestivum 'Chinese Spring'. Out of 6017 fragments amplified with 80 primer combinations in normal 'Chinese Spring', 24 were assigned to 1BS. Twelve of them clustered within a small region of the satellite known to be rich in RFLP (restriction fragment length polymorphism) markers. AFLPs in 1BS and in the whole genome were calculated between 'Chinese Spring' and T. spelta var. duhamelianum. The polymorphism rates in the satellite region (58.3%) and in the 1BS arm (45.8%) were much higher than the average rate for the whole genome (10.7%). Seven of the 12 AFLP markers in the satellite region were revealed to be specific to 'Chinese Spring' and could potentially be useful for genetic mapping in a segregation population of 'Chinese Spring' × T. spelta.Key words: AFLP, wheat, deletion mapping, 1BS satellite.
37
Matsui, K., Y. Kiryu, T. Komatsuda, N. Kurauchi, T. Ohtani, and T. Tetsuka. "Identification of AFLP makers linked to non-seed shattering locus (sht1) in buckwheat and conversion to STS markers for marker-assisted selection." Genome 47, no. 3 (June 1, 2004): 469–74. http://dx.doi.org/10.1139/g04-007.
Full textAbstract:
Shattering habit in buckwheat has two forms: brittle pedicel and weak pedicel. Brittle pedicel is observed in wild buckwheat, but not in cultivated buckwheat. Brittle pedicel in buckwheat is produced by two complementary, dominant genes, Sht1 and Sht2. The sht1 locus is linked to the S locus; almost all common buckwheat cultivars possess the allele sht1. To detect molecular makers linked to the sht1 locus, we used amplified fragment-length polymorphism (AFLP) analysis in combination with bulked segregant analysis of segregating progeny of a cross between a non-brittle common buckwheat and a brittle self-compatible buckwheat line. We screened 312 primer combinations and constructed a linkage map around the sht1 locus by using 102 F2 plants. Five AFLP markers were linked to the sht1 locus. Two of these, e54m58/610 and e55m46/320, cosegregated with the sht1 locus without recombination. The two AFLP markers were converted to STS markers according to the sequence of the AFLPs. The STS markers are useful for marker-assisted selection of non-brittle pedicel plants and provides a stepping-stone for map-based cloning and characterization of the gene encoding non-brittle pedicel.Key words: Fagopyrum esculentum, brittle pedicel, self-compatibility, bulked segregant analysis.
38
García-Martínez, Santiago, Lorella Andreani, Marta Garcia-Gusano, Filippo Geuna, and Juan J. Ruiz. "Evaluation of amplified fragment length polymorphism and simple sequence repeats for tomato germplasm fingerprinting: utility for grouping closely related traditional cultivars." Genome 49, no. 6 (June 1, 2006): 648–56. http://dx.doi.org/10.1139/g06-016.
Full textAbstract:
Cultivated tomato (Solanum lycopersicum L.) germplasm shows limited genetic variation. Many DNA marker systems have been used for genetic diversity studies in wild and cultivated tomatoes, but their usefulness for characterizing phenotypic differences among very closely related cultivars remains uncertain. We have used 19 selected simple sequence repeat (SSR) markers and 7 amplified fragment length polymorphism (AFLP) primer combinations to characterize 48 cultivars of tomato, mainly traditional cultivars from the south-east of Spain. The main types were Solanum lycopersicum L. 'Muchamiel', 'De la pera', and 'Moruno'. The robustness of the dendrograms and the discrimination power reached with each marker type were similar. Unique fingerprinting even of the most closely related tomato cultivars could be obtained using a combination of some SSR and AFLP markers. A better grouping of the 'Muchamiel' cultivars was observed with SSR markers, whereas the grouping of cultivars of 'De la pera' type was best achieved with AFLPs. However, both types of markers adequately grouped cultivars of the main types, confirming the utility of SSR and AFLP markers for the identification of traditional cultivars of tomato.Key words: genetic variability, molecular markers, Solanum lycopersicum.
39
Santos, Carlos A. F., and Philipp W. Simon. "Merging Carrot Linkage Groups based on Conserved Dominant AFLP Markers in F2 Populations." Journal of the American Society for Horticultural Science 129, no. 2 (March 2004): 211–17. http://dx.doi.org/10.21273/jashs.129.2.0211.
Full textAbstract:
Markers were placed on linkage groups, ordered, and merged for two unrelated F2 populations of carrot (Daucus carota L.). Included were 277 and 242 dominant Amplified fragment-length polymorphism (AFLP) markers and 10 and eight codominant markers assigned to the nine linkage groups of Brasilia × HCM and B493 × QAL F2 populations, respectively. The merged linkage groups were based on two codominant markers and 28 conserved dominant AFLP markers (based upon sequence and size) shared by both populations. The average marker spacing was 4.8 to 5.5 cM in the four parental coupling phase maps. The average marker spacing in the six merged linkage groups was 3.75 cM with maximum gaps among linkage groups ranging from 8.0 to 19.8 cM. Gaps of a similar size were observed with the linkage coupling phase maps of the parents, indicating that linkage group integration did not double the bias which comes with repulsion phase mapping. Three out of nine linkage groups of carrot were not merged due to the absence of common markers. The six merged linkage groups incorporated similar numbers of AFLP fragments from the four parents, further indicating no significant increase in bias expected with repulsion phase linkage. While other studies have merged linkage maps with shared AFLPs of similar size, this is the first report to use shared AFLPs with highly conserved sequence to merge linkage maps in carrot. The genome coverage in this study is suitable to apply quantitative trait locus analysis and to construct a cross-validated consensus map of carrot, which is an important step toward an integrated map of carrot.
40
Barchi, Lorenzo, Sergio Lanteri, Ezio Portis, Anikò Stàgel, Giampiero Valè, Laura Toppino, and Giuseppe Leonardo Rotino. "Segregation distortion and linkage analysis in eggplant (Solanum melongena L.)." Genome 53, no. 10 (October 2010): 805–15. http://dx.doi.org/10.1139/g10-073.
Full textAbstract:
An anther-derived doubled haploid (DH) population and an F2 mapping population were developed from an intraspecific hybrid between the eggplant breeding lines 305E40 and 67/3. The former incorporates an introgressed segment from Solanum aethiopicum Gilo Group carrying the gene Rfo-sa1, which confers resistance to Fusarium oxysporum ; the latter is a selection from an intraspecific cross involving two conventional eggplant varieties and lacks Rfo-sa1. Initially, 28 AFLP primer combinations (PCs) were applied to a sample of 93 F2 individuals and 93 DH individuals, from which 170 polymorphic AFLP fragments were identified. In the DH population, the segregation of 117 of these AFLPs as well as markers closely linked to Rfo-sa1 was substantially distorted, while in the F2 population, segregation distortion was restricted to just 10 markers, and thus the latter was chosen for map development. A set of 141 F2 individuals was genotyped with 73 AFLP PCs (generating 406 informative markers), 32 SSRs, 4 tomato RFLPs, and 3 CAPS markers linked to Rfo-sa1. This resulted in the assignment of 348 markers to 12 major linkage groups. The framework map covered 718.7 cM, comprising 238 markers (212 AFLPs, 22 SSRs, 1 RFLP, and the Rfo-sa1 CAPS). Marker order and inter-marker distances in this eggplant map were largely consistent with those reported in a recently published SSR-based map. From an eggplant breeding perspective, DH populations produced by anther culture appear to be subject to massive segregation distortion and thus may not be very efficient in capturing the full range of genetic variation present in the parental lines.
41
Terauchi, Ryohei, and Günter Kahl. "Mapping of the Dioscorea tokoro genome: AFLP markers linked to sex." Genome 42, no. 4 (August 1, 1999): 752–62. http://dx.doi.org/10.1139/g99-001.
Full textAbstract:
Two framework linkage maps were constructed for the genome of the dioecious wild yam species Dioscorea tokoro. The pseudo-testcross strategy was employed, using 271 amplified fragment length polymorphisms (AFLPs), five sequence-tagged microsatellite sites, one isozyme, and one morphological marker. For the two parents DT7 and DT5 used in the cross, 13 and 12 linkage groups, respectively, were identified. The total map lengths were 669 and 613 cM, respectively, for DT7 and DT5, which cover more than 75% of the D. tokoro genome. Ten AFLP markers heterozygous only in the male parent showed tight linkages with the sex of its progeny, which suggests that male is the heterogametic sex (XY) and the female is the homogametic sex (XX).Key words: Dioscorea tokoro, yam, linkage map, AFLP, sex determination.
42
Xu, D. H., and T. Ban. "Conversion of AFLP markers associated with FHB resistance in wheat into STS markers with an extension-AFLP method." Genome 47, no. 4 (August 1, 2004): 660–65. http://dx.doi.org/10.1139/g04-022.
Full textAbstract:
Amplified fragment length polymorphism (AFLP) has proven a powerful tool for tagging genes or quantitative trait loci (QTLs) of interest in plants. However, conversion of AFLP markers into sequence-tagged site (STS) markers is technically challenging in wheat owing to the complicated nature of its genome. In this study, we developed an "extension-AFLP" method to convert AFLP markers associated with Fusarium head blight (FHB) resistance into STS markers. When an AFLP marker of interest was detected with an EcoRI+3MseI+4-selective primer combination, the PCR product was used as a template for an additional selective amplification with four primer pairs, in which one additional selective base (either A, C, G, or T) was added to the 3' end of one of the two primers. The extended primer pair that produced the targeted band was further extended by adding each of the four selective nucleotide bases for the next round of selective amplification. Extension selective amplification was performed until the target bands became clear enough for subsequent cloning and sequencing. By using the extension-AFLP method, we successfully converted two AFLP markers located on chromosome 3BS and associated with FHB resistance into STS markers. Our results indicated that the extension-AFLP method is an efficient approach for converting AFLP markers into STS markers in wheat. The developed STS markers might be used for marker-assisted selection (MAS) for FHB resistance in wheat breeding programs.Key words: extension-AFLP, wheat, STS, FHB resistance.
43
Yusuf, Hajara Oyiza, Joshua Olu, Shakirat O. AJENIFUJAH- SOLEBO, Rebecca Oziohu OMOSIMUA, Charity Irekpita AKHIGBE, and Okechukwu Ibeh BARTHOLOMEW. "Identification and Sequencing of aflP (omt) and aflD (Nor-1) Genes from Aspergillus flavus Species isolated from Maize (Zea mays L.), Obtained Across Abuja, Nigeria." Journal of Biochemistry and Molecular Biology 1, no. 1 (October 20, 2022): 10–19. http://dx.doi.org/10.36108/jbmb/2202.10.0120.
Full textAbstract:
This study aims to examine aflD (Nor-1) and aflP (omt) genes that could be present in species of Aspergillus flavus isolated from varieties of maize seeds obtained across Abuja, Nigeria, utilizing the PCR method and Sanger sequencing method. The DNA extraction of16 isolates (14 were procured maize varieties and 2 known aflatoxin infected varieties of yellow and white maize from the laboratory to serve as positive control) was carried out with Zymo, D6005 DNA extraction kit. All 16 A. flavus isolates were tested for amplification of Aflatoxin genes with oligonucleotide primers (FwOmt-1:5’, RvOmt-1:5’, FwNor-1: 5′, and Rv Nor-1: 5′). The PCR analysis was carried out with a DreamTaq Green PCR Master Mix (2x). The sanger sequencing was carried out using ExoSAP-IT™ Express PCR Product Cleanup Sequencing Kit. The findings of this study showed that all A. flavus isolated from the various maize varieties had the presence of NOR (afl D) gene except for the A. flavus isolated from Maize-Samaz 15, Maize-Oba super 39 and Maize-Oba super 6 (UNIAbuja). In addition, Maize-DK 777, Maize-Samaz 52 (Abaji) and Maize-Samaz 37 varieties had the OMT (aflP) gene. This study has demonstrated that an Aspergillus flavus attack may either be accompanied with aflatoxin production or not, with the Maize-Oba super 39, Maize-Samaz 15 and Maize-Oba super 6 (UNIAbuja) although infected with A. flavus they had no aflD and aflP gene present in them. In addition, this study revealed that the maize varieties across Abuja were prone to A. flavus contamination which have tendency to produce aflatoxin.
44
GITZENDANNER, M. A., and P. S. SOLTIS. "GENETIC VARIATION IN RARE AND WIDESPREAD LOMATIUM SPECIES (APIACEAE): A COMPARISON OF AFLP AND SSCP DATA." Edinburgh Journal of Botany 58, no. 2 (June 2001): 347–56. http://dx.doi.org/10.1017/s0960428601000671.
Full textAbstract:
Plant conservation genetics has been hampered by a lack of markers for studies of levels and patterns of variation in rare species. We investigated the levels of variation in several rare and widespread species of the western North American genus Lomatium Raf. (Apiaceae) using two relatively new molecular markers: AFLPs and single-strand conformation polymorphisms (SSCPs). For each species, approximately 150 AFLP loci have been scored, yielding estimates of species-level percent polymorphic loci in rare species ranging from near zero to over 80%. Levels of AFLP diversity were similar in two of the rare species, L. bradshawii (Rose ex Mathias) Mathas & Constance and L. ochocense Helliwell & Constance, and the widespread species. The third rare species, L. cookii Kagan, which has small populations, has low levels of diversity based on AFLPs. We also examined nucleotide diversity at the single-copy nuclear-DNA locus glyceraldehyde 3-phosphate dehydrogenase (Gap-C). PCR-amplified segments were analysed for allelic variation using SSCPs, and intrapopulational nucleotide polymorphisms were identified in both L. bradshawii and L. cookii. In the 211bp segment of Gap-C analysed, five nucleotide sites were segregating within populations of L. bradshawii and two in L. cookii.
45
Morré, Servaas A., Jacobus M. Ossewaarde, Paul H. M. Savelkoul, Jeroen Stoof, Chris J. L. M. Meijer, and Adriaan J. C. van den Brule. "Analysis of Genetic Heterogeneity in Chlamydia trachomatis Clinical Isolates of Serovars D, E, and F by Amplified Fragment Length Polymorphism." Journal of Clinical Microbiology 38, no. 9 (2000): 3463–66. http://dx.doi.org/10.1128/jcm.38.9.3463-3466.2000.
Full textAbstract:
Amplified fragment length polymorphism (AFLP) fingerprinting of clinical isolates of Chlamydia trachomatis serovars D, E, and F showed a low percentage of genetic heterogeneity, but clear differences were found. Isolates from index patients and partners had identical AFLP patterns and AFLP markers. Characterization of these AFLP markers could give more insight into the differences in virulence and clinical course of C. trachomatis infections.
46
Lindstedt, Bjørn-Arne, Even Heir, Traute Vardund, Kjetil K. Melby, and Georg Kapperud. "Comparative Fingerprinting Analysis ofCampylobacter jejuni subsp. jejuni Strains by Amplified-Fragment Length Polymorphism Genotyping." Journal of Clinical Microbiology 38, no. 9 (2000): 3379–87. http://dx.doi.org/10.1128/jcm.38.9.3379-3387.2000.
Full textAbstract:
Amplified-fragment length polymorphism (AFLP) analysis with the endonucleases BglII and MfeI was used to genotype 91 Campylobacter jejuni subsp. jejunistrains from outbreaks and sporadic cases. AFLP-generated fragments were labeled with fluorescent dye and separated by capillary electrophoresis. The software packages GeneScan and GelCompar II were used to calculate AFLP pattern similarities and to investigate phylogenetic relationships among the genotyped strains. The AFLP method was compared with two additional DNA-based typing methods, pulsed-field gel electrophoresis (PFGE) using SmaI and restriction fragment length polymorphism analysis on PCR products (PCR-RFLP) of theflaA and flaB genes. We found that AFLP analysis of C. jejuni strains is a rapid method that offers better discriminatory power than do both PFGE and PCR-RFLP. AFLP and, to a lesser extent, PCR-RFLP could differentiate strains within the same PFGE profiles, which also makes PCR-RFLP an alternative to PFGE. We were able to clearly distinguish 9 of 10 recognized outbreaks by AFLP and to identify similarities among outbreak and sporadic strains. Therefore, AFLP is suitable for epidemiological surveillance ofC. jejuni and will be an excellent tool for source identification in outbreak situations.
47
Meng, Fanjuan, Mu Peng, and Fachun Guan. "Amplified Fragment Length Polymorphism Analysis of Genetic Diversity and Relationships of Wild and Cultivated Peach (Prunus persica L.)." HortScience 50, no. 1 (January 2015): 44–50. http://dx.doi.org/10.21273/hortsci.50.1.44.
Full textAbstract:
To date, a narrow genetic base is a serious obstacle in peach (Prunus persica L.) production. Wild peach resources are useful germplasms for breeding new cultivars. In this study, amplified fragment length polymorphisms (AFLPs) were used to analyze the genetic diversity and relationships of wild and cultivated peach germplasms. These results showed that AFLP is an efficient technique for identifying the genetic relationships of wild and cultivated peach. Thirteen AFLP primer combinations generated a total of 377 scorable and clear fragments, all of which (100%) were polymorphic. Moreover, the polymorphism information content (PIC) values ranged from 0.91 to 0.96 with a mean of 0.95. The results of the principal component analysis (PCoA) largely corresponded to those obtained using cluster analysis. The three principal axes accounted for 2.6%, 5.79%, and 25.26% of the total variation, respectively. In conclusion, wild peach germplasms should receive special attention to ensure their conservation.
48
van Eldere, Johan, Paul Janssen, Annette Hoefnagels-Schuermans, Stefaan van Lierde, and Willy E. Peetermans. "Amplified-Fragment Length Polymorphism Analysis versus Macro-Restriction Fragment Analysis for Molecular Typing ofStreptococcus pneumoniae Isolates." Journal of Clinical Microbiology 37, no. 6 (1999): 2053–57. http://dx.doi.org/10.1128/jcm.37.6.2053-2057.1999.
Full textAbstract:
Forty-eight pneumococci were genotyped by on-line laser fluorescence amplified-fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE) analysis of chromosomal restriction fragments. Overall, the data generated by the two methods corresponded well. However, with AFLP, clusters were delineated at a higher similarity level, and isolate differentiation was more pronounced. AFLP and PFGE were equally efficient for assessing intraserotype diversity. We conclude that AFLP is a useful alternative to PFGE.
49
Xiao, Shushu, Jinsong Xu, Yuan Li, Lei Zhang, Shijun Shi, Shuwen Shi, Jiangsheng Wu, and Kede Liu. "Generation and mapping of SCAR and CAPS markers linked to the seed coat color gene in Brassica napus using a genome-walking technique." Genome 50, no. 7 (July 2007): 611–18. http://dx.doi.org/10.1139/g07-044.
Full textAbstract:
The yellow seed coat trait in No. 2127-17, a resynthesized purely yellow Brassica napus line, is controlled by a single partially dominant gene, Y. A double-haploid population derived from the F1 of No. 2127-17 × ‘ZY821’ was used to map the seed coat color phenotype. A combination of AFLP analysis and bulked segregant analysis identified 18 AFLP markers linked to the seed coat color trait. The 18 AFLP markers were mapped to a chromosomal region of 37.0 cM with an average of 2.0 cM between adjacent markers. Two markers, AFLP-K and AFLP-H, bracketed the Y locus in an interval of 1.0 cM, such that each was 0.5 cM away from the Y locus. Two other markers, AFLP-A and AFLP-B, co-segregated with the seed color gene. For ease of use in breeding programs, these 4 most tightly linked AFLP markers were converted into reliable PCR-based markers. SCAR-K, which was derived from AFLP-K, was assigned to linkage group 9 (N9) of a B. napus reference map consisting of 150 commonly used SSR (simple sequence repeat) markers. Furthermore, 2 SSR markers (Na14-E08 and Na10-B07) linked to SCAR-K on the reference map were reversely mapped to the linkage map constructed in this study, and also showed linkage to the Y locus. These linked markers would be useful for the transfer of the dominant allele Y from No. 2127-17 to elite cultivars using a marker-assisted selection strategy and would accelerate the cloning of the seed coat color gene.
50
Zhang, D., Z. Zhang, and K. Yang. "Identification of AFLP Markers Associated with Embryonic Root Development in Populus tomentosa Carr." Silvae Genetica 56, no. 1-6 (December 1, 2007): 27–32. http://dx.doi.org/10.1515/sg-2007-0004.
Full textAbstract:
Abstract Embryonic root (radicle) development in the mature embryo following germination is essential for the formation of the root organ in plants. In this study a phenotype described by a lack of proper radicle development was identified in an intraspecific hybrid of Populus tomentosa Carr.. Association of this trait with Amplified Fragment Length Polymorphisms (AFLPs) markers was investigated in a segregating F1 population generated by intraspecific-controlled crossing between a highly fertile female P. tomentosa clone “5082” and a male P. tomentosa clone “JY”. A total of 3193 seeds were obtained, and the rate of germination found to be 48.74% at 15 to 20 days. 376 (24%) of seedlings were shown to lack a root organ following visual assessment of the developing radicle. Genetic regulation of this trait appeared to be via a single dominant gene or a set of tightly linked genes, based on the 3:1 ratio of the rooting versus nonrooting seed embryos. A Bulked Segregant Analysis approach using 5600 AFLP markers was applied to this population and revealed 2 AFLP markers, EcoRI + GAG/ Mse I + AAT-492 and EcoRI + GAG/Mse I + CCA-502, that were associated with the radicle development-controlling locus in P. tomentosa. The AFLP markers identified have potential for application in hybrid breeding via marker assisted selection, and provide a starting point for map based cloning of the radical development-controlling gene.