Relevant bibliographies by topics / Affinity adsorption

Academic literature on the topic 'Affinity adsorption'

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Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Affinity adsorption"

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Prasanna, Rajasekar R., and Mookambeswaran A. Vijayalakshmi. "Immobilized metal-ion affinity systems for recovery and structure–function studies of proteins at molecular, supramolecular, and cellular levels." Pure and Applied Chemistry 82, no. 1 (January 3, 2010): 39–55. http://dx.doi.org/10.1351/pac-con-09-01-18.

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Immobilized metal-ion affinity (IMA) adsorption is a collective term that is used to include all kinds of adsorptions where the metal ion serves as the characteristic and most essential part of adsorption center. Of all the IMA techniques, immobilized metal-affinity chromatography (IMAC) has been gaining popularity as the choice of purification technique for proteins. IMAC represents a separation technique that is primarily useful for proteins with natural surface exposed-histidine residues and for recombinant proteins with engineered histidine tag. This review also gives insight into other nonchromatographic applications of IMA adsorption such as immobilized metal-ion affinity gel electrophoresis (IMAGE), immobilized metal-ion affinity capillary electrophoresis (IMACE), and immobilized metal-ion affinity partitioning (IMAP).
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Hubble, J. "Stochastic Modeling of Affinity Adsorption." Biotechnology Progress 17, no. 3 (June 1, 2001): 565–67. http://dx.doi.org/10.1021/bp010014o.

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Ghose, S., G. M. Forde, and N. K. H. Slater. "Affinity Adsorption of Plasmid DNA." Biotechnology Progress 20, no. 3 (June 4, 2004): 841–50. http://dx.doi.org/10.1021/bp034257n.

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Shi, Wei, Yuanyuan Ma, Cunfeng Song, Hairong Jiang, Xiaoning Ru, Jing Tu, Shuhui Jiang, Jun Wang, and Dongtao Ge. "Affinity electromembrane: Electrically facilitated adsorption." Journal of Membrane Science 354, no. 1-2 (May 2010): 86–92. http://dx.doi.org/10.1016/j.memsci.2010.02.072.

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Undabeytia, T., E. Morillo, and C. Maqueda. "Adsorption of Cd and Zn on montmorillonite in the presence of a cationic pesticide." Clay Minerals 31, no. 4 (December 1996): 485–90. http://dx.doi.org/10.1180/claymin.1996.031.4.05.

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AbstractThe adsorption of Cd and Zn on a standard montmorillonite (SAZ-1) in the presence of the cationic pesticide chlordimeform, both in solution (simultaneous adsorption) and when the pesticide is previously adsorbed on the clay (successive adsorption) has been studied. The adsorption of Zn decreases when the chlordimeform concentration increases in both simultaneous and successive adsorptions, following the sequence: chlordimeform-free solutions > successive > simultaneous. In the adsorption of Cd the sequence is different: successive > chlordimeform-free solutions > simultaneous, indicating that a small amount of pesticide adsorbed favours Cd adsorption. In all cases, the apparent affinity of the metal for adsorption on montmorillonite, on the basis of distribution coefficients, KD, is higher for lower metal surface coverage, and decreases largely with the amount of the metal adsorbed. This indicates the existence of high affinity sites on the clay, probably those of variable charge edge regions. The contribution of edge and interlamellar positions to adsorption of Zn and Cd on SAZ-1 has been studied.
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BOI, C., F. CATTOLI, R. FACCHINI, M. SORCI, and G. SARTI. "Adsorption of lectins on affinity membranes." Journal of Membrane Science 273, no. 1-2 (March 31, 2006): 12–19. http://dx.doi.org/10.1016/j.memsci.2005.12.011.

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Bayraktaroğlu, Melis, Hande Orhan, Sinem Evli, Sinan Akgöl, Deniz Aktaş Uygun, and Murat Uygun. "Lectin attached affinity cryogels for amyloglucosidase adsorption." Journal of Carbohydrate Chemistry 37, no. 5 (June 13, 2018): 302–17. http://dx.doi.org/10.1080/07328303.2018.1487972.

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Yavuz, Handan, Mehmet Odabaşi, Sinan Akgöl, and Adil Denizli. "Immobilized metal affinity beads for ferritin adsorption." Journal of Biomaterials Science, Polymer Edition 16, no. 5 (January 2005): 673–84. http://dx.doi.org/10.1163/1568562053783713.

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Pechenyuk, S. I., Yu P. Semushina, and L. F. Kuz’mich. "Adsorption affinity of anions on metal oxyhydroxides." Russian Journal of Physical Chemistry A 87, no. 3 (February 3, 2013): 490–96. http://dx.doi.org/10.1134/s0036024413030205.

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Tolner, Berend, Lisa Smith, Richard H. J. Begent, and Kerry A. Chester. "Expanded-bed adsorption immobilized-metal affinity chromatography." Nature Protocols 1, no. 3 (August 2006): 1213–22. http://dx.doi.org/10.1038/nprot.2006.127.

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Dissertations / Theses on the topic "Affinity adsorption"

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Mayes, Andrew Geoffrey. "Quantitative aspects of affinity adsorption." Thesis, University of Bath, 1992. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303403.

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Horstmann, Brenda Joan. "Affinity adsorption on agarose matrices." Thesis, University of Cambridge, 1989. https://www.repository.cam.ac.uk/handle/1810/250951.

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Clemmitt, Robert Howard. "Metal affinity purification strategies for expanded bed adsorption." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621608.

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Taşcı, Yasemin. "Modeled Affinity Constants for Phosphorus Adsorption and Desorption due to Saltwater Intrusion." Scholar Commons, 2019. https://scholarcommons.usf.edu/etd/7966.

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It is important to understand the processes that regulate phosphorus (P) fluxes to coastal environments, because P is an important nutrient in coastal ecosystems. Phosphorus adsorbs to the surface of minerals in sediment and bedrock, and an influx of seawater can cause some of that P to desorb, raising the P concentration of ambient water. Although seawater-induced P desorption is thought to be an important source of P to coastal environments, the chemical reactions that underlie it have not been established. Previous work provides some relevant surface reactions and associated affinity constants between various aqueous P species and the surface of calcite and in dilute calcium carbonate-P solutions. However, these reactions with their respective affinity constants from the literature fail to predict the behavior of P with calcite in seawater. In this study, we conducted a series of batch experiments involving both adsorption and desorption of P in seawater, freshwater, dilute seawater, and mixtures of seawater and freshwater. We used these results in the geochemical model PHREEQC and the parameter estimation model PEST to optimize the affinity constants for the existing surface reactions. We found that after making minor adjustments to the affinity constants, the existing surface complexation models of calcite surface reactions from the published literature are sufficient to explain seawater-induced P desorption. Specifically, our results suggest that CaPO4- and either CaHPO40 or HPO42- may be important species in the P adsorption/desorption reactions in freshwater-seawater mixing.
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Horn, Carsten. "Downstream processing with affinity chromatography : a study of a continuous process for biospecific adsorption." Thesis, University of South Wales, 1993. https://pure.southwales.ac.uk/en/studentthesis/downstream-processing-with-affinity-chromatography-a-study-of-a-continuous-process-for-biospecific-adsorption(acd33b1a-fed5-47b3-9b01-b389810c1466).html.

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A polymer matrix of hydroxy ethyl methacrylate - ethylene glycol dimethacrylate was synthesised and coupled with the ligand p-aminobenzamidine. Although the adsorbent displayed sufficient affinity towards trypsin, the overall adsorption performance was not satisfactory. Subsequent studies were carried out with a p-aminobenzamidine silica adsorbent, which gave far superior results. Amounts of up to 35 mg of trypsin per g of silica could be adsorbed from a porcine pancreatic extract at buffer conditions of 0.05 M Tris, 0.5 M NaCl, pH 8. Complete elution was achieved with a buffer of 0.1 M acetic acid, pH 3. The system was characterised by fixed bed breakthrough studies of the adsorption, wash and desorption step. A method of data analysis was developed and applied to the experimental results. Axial dispersion could be identified as the predominant 'mass transfer' mechanism. A simulation model was build, based on the data analysis results. The model was verified successfully against the experimental data. Continuous separation experiments were performed with three fluidised beds, namely an adsorption, desorption and wash column, with circulation of solids between the columns. Purities of 83% and 66% were achieved in these runs with complete recovery of trypsin. A continuous process model, also based on the data analysis results and on fluidised bed characteristics, was found to agree fairly well with the experimental data. Simulation experiments were conducted to find the optimum configuration of the process. Best performances with respect to product purity were obtained for an adsorption column height of 3 cm and a wash column height of 4 cm. Step experiments revealed that the process is relatively easy to control if the solid flow rate is chosen as the control parameter.
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Wen, Zhenzhen. "Fundamental studies of affinity separation of glycoproteins and its combination with expanded bed adsorption technique." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=980855373.

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Niranjane, Ajay Pundaiikrao, and ajay niranjane@gmail com. "Screening diverse cellulase enzymes from the white rot fungus Phlebia gigantea for high activity and large scale applications." RMIT University. Applied Sciences, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080513.150257.

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Cellulosic biomass is the major organic matter produced in the biosphere. The biodegradation of this cellulosic material is achieved by enzymatic activities of the cellulose degrading microorganisms. These organisms usually express a complex extracellular or a membrane bound cellulolytic system comprising combination of several cellulase enzymes. Cellulases are the group of hydrolytic enzymes capable of hydrolysing insoluble cellulose to glucose. Phlebia gigantea is an aggressive white rot basidiomycete with ability to tolerate resinous extracts on freshly cut wood and higher growth rate. This helps the fungus to colonise the sapwood preventing other fungi from becoming established. Early research on the cellulase system of this organism reported the presence of a cellulase system composed of P-glucosidase, endoglucanase and a cellobiohydrolase. Based on these unpublished studies, our aim was to obtain a complete sequence of putative cellobiohydrolase I (CbhI) from this organism. Attempts to identify and isolate the cellulase gene resulted in an incomplete cDNA sequence of I 154 bp. To understand the cellulase system, expression and regulation of the cellulase enzymatic activity was examined for incubation of P. gigantea on substrates glucose, xylose, Avicel, carboxymethyl cellulose and cellobiose. The pH, total protein and biomass production results indicated that the capacity of P. gigantea to degrade cellulose is dependent upon the nature of the carbon source and the regulation of the cellulase synthesis is repressed in the presence of simple sugars like glucose and xylose. The study employed the highly effective method of purification by affinity adsorption and purified cellulase complex in large quantity. Characterisation of the kinetic properties of this cellulase complex revealed that the rate of cellulase catalysis were optimum at pH 5.0 and temperature 50GC. The purified complex was comprised of multiple proteins and demonstrated significant CMCase and CBHase activity on zymogram analysis. The purified cellulase complex was characterised by 2D gel electrophoresis and by peptide mass finger printing using MALDI-TOF massspectrometry analysis. The 2D gel analysis of the purified cellulase complex showed 15 spots within the range of pI 3.5 to pI 7 and the molecular weight between 20KDa to 100KDa. Three protein spots were selected based on the IEF and SDS zymogram and identified using MALDI-TOF MS analysis. These proteins were identified based on the peptide mass data belonging to the 6-phospho-a-glucosidase, p-glucosidase and glycosyl hydrolase family 13 a-amylase or pullulanases, suggesting the divergent evolution of specific cellulase proteins. This study showed P. gigantea as a potential cellulase source and the cellulase complex secreted by the induction of substrate, comprises a variety of enzymes related to hydrolysis of cellulose biomass. It is evident from this and previous studies that P. gigantea cellulase complex comprises of a specific set of enzymes that possess the ability to degrade crystalline cellulose and is one of the first organisms to colonise freshly cut wood. Further studies on the cellulase system of this primary colonist may open up the prospects to utilise this organism as the potential onsite bioreactor agent, pre-treating the biomass and increasing the economic feasibility of the industrial bioenergy processes.
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Gonzalez, Ortega Omar. "STUDY AND CHARACTERIZATION OF DUAL-FUNCTION AFFINITY CHROMATOGRAPHIC ADSORBENTS HAVING SIZE EXCLUSION AND ADSORPTION PROPERTIES TO ISOLATE, PURIFY AND RECOVER SMALL BIOMOLECULES FROM COMPLEX BIOLOGICAL MIXTURES." Diss., The University of Arizona, 2010. http://hdl.handle.net/10150/195896.

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In this work, the main emphasis of the research concerns the development of isolation and purification methods of biomolecules from biological fluids. Several separation techniques were incorporated in chromatographic gels to obtain multifunctional hybrid chromatographic separation media for proteins, peptides and amino acid isolation and purification.In the first part of the research, several chelating agents were synthesized and their effectiveness to purify immunoglobulins using Immobilized Metal Affinity Chromatography (IMAC) was investigated. Ethylenediamine triacetic acid (TED) with immobilized copper resulted in the most effective in terms of purification and protein capacities.The next part of the work involved the development of hybrid chromatographic media that combines protein specific adsorption with sharp controlled size access permeation. This was accomplished by incorporating two types of ligand derivatives, one that permits the permeation of only certain molecular size range compounds, and a second one that specifically binds target biomolecules among the compounds of that specific molecular size range. Hybrid systems included binding ligands for Immobilized Metal Affinity Chromatography (IMAC), Ion Exchange Chromatography (IEX) and Hydrophobic Interaction Chromatography (HIC) combined with a controlled access polymer at different densities such as polyethylene glycol (PEG) and dextran derivatives. In general, low grafting density of high molecular weight PEG was found to be as effective as high grafting density of low molecular weight PEG in the rejecting properties of the semi-permeable synthesized media.Theoretical and experimental batch adsorption studies were also performed with the hybrid media and a mathematical model was developed to study the uptake of proteins under specific conditions of controlled permeation.In the last stage of this work, chelating surfactants were synthesized and used as reversible affinity ligands on reversed phase adsorbents for protein separations.One of the main accomplishments of this research was the development of separation media for small molecular size compounds from larger molecules and from complex biological systems. Applications of special interest will include the isolation and purification of solutes, such as metal ions, toxins, drugs, biomolecules, including proteins, biotoxins, nucleic acids, peptides, hormones, and biomarkers from biological fluids (such as human serum, urine, etc.) and from aqueous solutions.
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Duarte, Isa Santos. "Adsorção de ige humana a partir de amostras sericas ou plasmaticas em lectinas imobilizadas em agarose." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267686.

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Orientadores : Sonia Maria Alves Bueno, Ricardo de Lima Zollner
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Quimica
Made available in DSpace on 2018-08-06T11:50:08Z (GMT). No. of bitstreams: 1 Duarte_IsaSantos_D.pdf: 749498 bytes, checksum: 83d66753c75e7828fd4a8b858aecf473 (MD5) Previous issue date: 2006
Resumo: A alergia é uma enfermidade do sistema imunológico que afeta aproximadamente de 20 a 30% da população mundial. Dentre as reações alérgicas, a reação de hipersensibilidade imediata é mediada pelas imunoglobulinas E (IgE). Os indivíduos geneticamente predispostos a manifestar reações por hipersensibilidade imediata e polissensibilizados aos alérgenos ambientais são considerados atópicos e, geralmente, possuem teores de IgE total até 10.000 vezes mais elevados do que as pessoas não-atópicas. O conhecimento das interações entre a IgE e ligantes de afinidade pode levar ao desenvolvimento de novos tratamentos da hipersensibilidade imediata, por exemplo a terapia de adsorção seletiva através de circulação extracorpórea, assim como ao desenvolvimento de métodos de obtenção de IgE purificada, para aplicação nas áreas de diagnóstico, pesquisa molecular, dentre outras. Os adsorventes empregados na terapia de adsorção seletiva, bem como na purificação de IgE, geralmente são anticorpos anti-IgE imobilizados em agarose, os quais são de alto custo e difícil obtenção. Este trabalho avaliou o desempenho de adsorventes alternativos ao Sepharose-anti-IgE, visando a remoção de IgE total e específica aos ácaros Dermatophagoides pteronyssinus e Blomia tropicalis de amostras plasmáticas e a preparação de soluções enriquecidas em IgE, como uma das etapas do processo de purificação de IgE. Os adsorventes estudados constituíram-se de lectinas (concanavalina A e Lens culinaris), aminas (poli-L-lisina e aminohexil) e o aminoácido D-triptofano, imobilizados em agarose. Dentre eles, o gel agarose-Lens culinaris mostrou-se o mais promissor para aplicação na terapia de adsorção seletiva de IgE e o gel Sepharose-concanavalina A mostrou-se o mais adequado para ser usado na obtenção de soluções enriquecidas em IgE. Experimentos cromatográficos foram realizados visando estabelecer condições experimentais (velocidade superficial, número de passagens de plasma pela coluna, temperatura e razão entre volume de plasma e volume de leito) mais favoráveis à adsorção de IgE em agarose-Lens culinaris. Posteriormente, essas condições foram utilizadas nos experimentos de simulação in vitro de circulação extracorpórea, nos quais o gel agarose-Lens culinaris removeu de 40,7 a 42,8% de IgE¿s total e específicas. A obtenção da solução enriquecida em IgE foi realizada por meio de duas etapas cromatográficas, empregando-se os princípios de afinidade (colunas agarosejacalina e Sepharose-concanavalina A) e de exclusão por tamanho (permeação em gel). A solução final enriquecida em IgE obtida, continha como principais impurezas, IgA e IgG. Como resultado das duas etapas, 36,6% de IgE foi recuperada e o fator de enriquecimento em IgE, em relação a IgA, IgG, IgM e albumina, foi de 75,8. Apesar do gel Sepharose-anti- IgE apresentar desempenho melhor tanto na remoção quanto na purificação de IgE, os adsorventes agarose-Lens culinaris e Sepharose-concanavalina A apresentam custos mais atrativos
Abstract: Allergy is a disorder of the imune system, affecting approximately 20%-30% of the general population. Among allergic reactions, immediate hypersensitivity is mediated by immunoglobulin E (IgE). Individuals that have a genetic predisposition for responses to immediate hypersensitivity are named atopic and generally have elevated serum IgE concentration, up to 10,000-fold higher than in the normal population. The knowledge of the interactions between IgE and affinity ligands may lead to the development of new methods of treatment for immediate hypersensitivity, for example, IgE selective adsorption therapy through extracorporeal circulation, as well as to new methods for obtaining purified IgE, which is employed in diagnostic and in molecular research. The adsorbents employed in IgE selective adsorption therapy, as well as in IgE purification, are usually antibodies anti-IgE immobilized on agarose, which have high costs and are difficult to obtain. This work assessed the performance of adsorbents (alternative to Sepharose-anti-IgE) for the removal of total IgE and IgE specific for the airbone allergens Dermatophagoides pteronyssinus and Blomia tropicalis from plasma samples, as well as in the production of IgE enriched solutions, considered as a step of IgE purification. The adsorbents studied were lectins (concanavalina A and Lens culinaris), amines (poli-L-lisina e aminohexil) and the aminoacid D-tryptophan, all of them immobilized on agarose. Among them, Lens culinaris-agarose showed the best performance for IgE selective adsorption therapy, and Sepharose¿concanavalin A was considered the most appropriate for the production of IgE enriched solutions. Chromatographic experiments were accomplished in order to determine operating conditions (superficial velocity, number of times the plasma passed through the column, temperature, and ratio of plasma volume to bed volume) more favorable to IgE adsorption on Lens culinaris-agarose. The selected conditions were utilized in in vitro simulation assays of extracorporeal circulation, in which the Lens culinaris-agarose removed from 40.7% to 42.8% of total and specific IgE. The production of IgE enriched solutions was carried out with two chromatographic steps, employing affinity (columns jacalin-agarose and Sepharose-concanavalin A) and size exclusion (gel permeation) principles. The IgE enriched final solution contained IgA and IgG as the major impurities. As a result of both steps, 36.6% of IgE was recovered and the IgE enrichement number concerning IgA, IgG, IgM, and albumin was 75.8. Despite Sepharose-anti-IgE has better performance in removal and purification of IgE, Lens culinaris-agarose and Sepharose-concanavalin A adsorbents have more attractive costs
Doutorado
Desenvolvimento de Processos Biotecnologicos
Doutora em Engenharia Quimica
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Nanakassé, Sidiki. "Sur l'utilisation du gel de silice dans des machines frigorifiques à affinité : adsorption de l'eau et du méthanol." Dijon, 1986. http://www.theses.fr/1986DIJOS070.

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Etude théorique de l'évolution morphologique du gel de silice (Rhône-Poulenc, Prolabo) sous l'action combinée de la température et de la vapeur d'adsorbat (eau, méthanol). Application à la réfrigération. Le système gel de silice-adsorbat (eau, méthanol) est très efficace dans le cas des faibles différences de température entre la source froide et la source chaude, et la source froide et la température de l'évaporateur; mais il est très sensible aux écarts de température au niveau de l'adsorbeur. La faible vitesse d'adsorption entraine quelques difficultés à utiliser ce système pour la mise en froid d'une charge. Par contre, il sera facilement utilisable pour la compensation des pertes thermiques.
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Books on the topic "Affinity adsorption"

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He, Lizhong. Self-assembly and fundamental aspects of affinity adsorption of glycoconjugates. Hamburg: Universität der Bundeswehr, 2002.

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Khan, Asad R. Characterisation of the adsorption affinity parameters of ionic oligomers by anion-exchange chromatography. Manchester: UMIST, 1997.

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Klein, Elias. Affinity membranes: Their chemistry and performancein adsorptive separation processes. New York: Wiley, 1991.

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Klein, Elias. Affinity membranes: Their chemistry and performance in adsorptive separation processes. New York: Wiley, 1991.

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Scouten, William H. Affinity chromatography: Bioselective adsorption on inert matrices (Chemical analysis). Sigma-Aldrich Co, 1992.

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Horn, Carsten Stefan. Downstream processing with affinity chromatography: A study of a continuous process for biospecific adsorption. 1993.

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Book chapters on the topic "Affinity adsorption"

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Odde, D. J. "Affinity adsorption." In Handbook of Downstream Processing, 70–89. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-1563-3_4.

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Scopes, Robert K. "Separation by Adsorption—Affinity Techniques." In Protein Purification, 187–237. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4757-2333-5_7.

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Wang, Henry Y., Patrick Evans, Kevin Dykstra, Somesh Nigam, and Gregory S. Hyatt. "Application of Affinity Adsorption in Antibiotic Production." In Bioproducts and Bioprocesses, 267–77. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74227-9_24.

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Nigam, Somesh C., and Henry Y. Wang. "Mathematical Modeling of Bioproduct Adsorption Using Immobilized Affinity Adsorbents." In ACS Symposium Series, 153–68. Washington, DC: American Chemical Society, 1986. http://dx.doi.org/10.1021/bk-1986-0314.ch011.

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Zieris, A., S. Prokoph, K. R. Levental, P. B. Welzel, K. Chwalek, K. Schneider, U. Freudenberg, and C. Werner. "Sustainable Growth Factor Delivery through Affinity-Based Adsorption to starPEG-Heparin Hydrogels." In ACS Symposium Series, 525–41. Washington, DC: American Chemical Society, 2012. http://dx.doi.org/10.1021/bk-2012-1120.ch024.

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Wen, J. J., E. Klein, and A. F. Spatola. "Evaluation of a new membrane support for peptide synthesis and affinity adsorption purification." In Peptides, 153–55. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0683-2_47.

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Nandakumar, Renu, Hessam Afshari, and Duane F. Bruley. "Analysis of Equilibrium Adsorption Isotherms for Human Protein C Purification by Immobilized Metal Affinity Chromatography." In Advances in Experimental Medicine and Biology, 191–99. Boston, MA: Springer US, 2003. http://dx.doi.org/10.1007/978-1-4757-6125-2_27.

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Potempa, L. A., M. Motie, B. Anderson, E. Klein, and U. Baurmeister. "Conjugation of a Modified Form of Human C-Reactive Protein to Affinity Membranes for Extracorporeal Adsorption." In Biologically Modified Polymeric Biomaterial Surfaces, 105–17. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-1872-9_14.

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Hammarström, L. G., I. Fängmark, P. G. Jönsson, P. R. Norman, A. L. Ness, S. L. McFarlane, and N. M. Osmond. "Strategies for Selection of Test Compounds in Structure-Affinity Modelling of Active Carbon Adsorption Performance: A Multivariate Approach." In Molecular Modeling and Prediction of Bioactivity, 293–94. Boston, MA: Springer US, 2000. http://dx.doi.org/10.1007/978-1-4615-4141-7_47.

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Kaliszan, Roman, Anna Kaliszan, Terence A. G. Noctor, William P. Purcell, and Irving W. Waine. "Mechanism of retention of benzodiazepines in affinity, reversed-phase and adsorption high-performance liquid chromatography in view of quantitative structure-retention relationships." In Trends in QSAR and Molecular Modelling 92, 109–11. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1472-1_14.

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Conference papers on the topic "Affinity adsorption"

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Yang, Yin-Jie, Ellis M. Shelley, Yi-Chia Liaw, Shing-Yi Suen, Min-Ying Wang, Eric D. Conte, Tai-Hong Cheng, Cheng-Chiang Huang, and Shin-Ying Chou. "Preparation of Polyacrylonitrile-based Immobilized Metal-Ion Affinity Membrane for Protein Adsorption." In 14th Asia Pacific Confederation of Chemical Engineering Congress. Singapore: Research Publishing Services, 2012. http://dx.doi.org/10.3850/978-981-07-1445-1_304.

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Funar-Timofei, Simona, Ludovic Kuruntzi, Walter Fabian, and Daniela Ionescu. "Structure-Affinity Modeling of Azo Dye Adsorption on Cellulose Fibre by MLR." In The 12th International Electronic Conference on Synthetic Organic Chemistry. Basel, Switzerland: MDPI, 2008. http://dx.doi.org/10.3390/ecsoc-12-01283.

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Al Ezzi, Amged, and Hongbin Ma. "Equilibrium Adsorption Isotherm Mechanism of Water Vapor on Zeolites 3A, 4A, X, and Y." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-72601.

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Many researchers have analyzed the equilibrium adsorption isotherm mechanism of water vapor molecules on 4A, 3A, Y and X zeolites for decades. The demand for moisture removal continues to increase along with the increasing stringency of requirements for product quality control and production energy efficiency. Due to the negative charge of the zeolite framework, exchangeable compensation cations such as Na+, Li+, K+, Ba2+, Mg2+, and Ca2+ might be added. These cations are located at different sites within the framework and with different concentrations. Each cation shows a strong affinity to bond with water molecules. Adsorption sites must show a characteristic energy signature corresponding to the adsorbed amount. The values of enthalpies and entropies at each site are different since the bonding strength between the moisture molecules and cations of the site is also different. These exchangeable cations have a very important contribution in determining the zeolites adsorptive properties and selectivity. This investigation of the water vapor adsorption mechanism reveals that size, location, and type of exchangeable compensation cations have a concrete relationship to the adsorption process and zeolite cage capacity.
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Zhang, Hai-Tao, Jie Han, Yong Xue, Hua-Li Nie, Li-Min Zhu, and C. Branford-White. "Surface Modification of Electrospun Nylon Nanofiber Based Dye Affinity Membrane and Its Application to Papain Adsorption." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5163544.

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Adams, G. A., and C. Hallée. "THROMBOSPONDIN ADSORPTION AND PLATELET ADHESION TO SURFACES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643589.

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Recent research into cell adhesion has focused on a tripeptide sequence arg-gly-asp (RGD) that is common to a number of cytoadhesive proteins such as von Willebrand factor, fibronectin and fibrinogen. We have previously reported that thrombospondin (TSP) inhibited platelet adhesion to RGD proteins. On further purification of TSP, the inhibitory activity separated away from the TSP. In this report, we demonstate that TSP adsorbs to surfaces and promotes platelet adhesion and thus may belong to this family of cytoadhesins. TSP was purified by heparin affinity chromatography, ammonium sulfate precipitation and sucrose gradient ultracentrifugation. Final preparations were free of TSP aggregates and gave one band on SDS-PAGE. Washed human platelets were radiolabelled, combined with red blood cells and perfused through protein-coated glass or polyethylene (PE) tubes for 7 mins. at a shear rate of 100 s™1. TSP supported platelet adhesion to the same level as fibrinogen (FG), but less than collagen. Platelets were spread but no thrombi were present on the TSP or FG-coated tubes while thrombi formed on collagen-coated tubes. Levels of adsorption were similar for purified solutions of radioiodinated FG or TSP on both glass and PE surfaces. The competitive interactions between these proteins during adsorption to surfaces indicated at equimolar amounts the TSP and FG both deposited on the surface but as FG:TSP ratio increased FG was preferentially adsorbed. These results indicate that TSP is a cytoadhesive protein for platelets when it is adsorbed to surfaces. Thus, the release of TSP from platelets may promote haemostasis or thrombogenesis.(supported by Ontario Heart Foundation, Canada)
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Riethorst, W., M. W. P. M. te Booy, T. Beugeling, A. Bantjes, J. Over, and W. G. van Aken. "THE ISOLATION OF COAGULATION FACTOR VIII FROM HUMAN BLOOD PLASMA BY AFFINITY CHROMATOGRAPHY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644059.

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The need for high quality concentrates of coagulation factor VIII (FVIII:C) for treatment of haemophilia A is increasing. As the purity of FVIII:C obtained with existing large scale methods is poor and yields are low, another method for the isolation of FVIII is being developed primarily to avoid losses incurred during cryoprecipitation.Affinity gels were prepared by derivatizing Sepharose CL 4B with different positively charged ligand-spacer combinations. The adsorption of FVIII as well as the von Willebrand factor (VWF) from human blood plasma onto these gels was measured by a one-stage assay for FVIII:C, and enzyme immuno assays (ELISA) for FVIII:CAG and VWF:AG using monoclonal antibodies. The influences of pH, conductivity, ligand density, geltplasma ratio, and length and composition of the spacer as well as the adsorption kinetics were studied to obtain information about the types of interactions responsible for bonding of FVIII to the gels. A combination of at least electrostatic and hydrophobic interactions was concluded to play a role in most cases.At optimal conditions more than 90 % of FVIII could be adsorbed batch-wise from plasma at room temperature in less than one hour with a gel:plasma ratio of 1:20 (i.e. 2.5 g dried gel/1 plasma). In different runs 65-75 % of the FVIII:C applied was recovered by column-wise elution with a salt gradient. The eluate contained less than 0.34 % of the protein applied, which implies that FVIII was purified 190 times. Using fresh-frozen plasma (0.8 IU FVIII/ml) as a starting material for this one-step procedure the final specific activity was 2.3 IU/mg, which is significantly better than that obtained for FVIII isolated by cryoprecipitation. Furthermore, the F. VIII:C to VWF ratio in the eluate was approximately 1:1. The isolated FVIII:C was stable at room temperature and the supernatant plasma appears suitable for further fractionation. It is concluded that this method is worth scaling up and its use for purification of FVIII from other sources is anticipated.
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Haghshenas, Alireza, and Mohammad Hamedpour. "Adsorption Modeling of CO2 and CH4 in Nanopores Using Simplified Local Density Model: Application in Enhanced Hydrocarbon Recovery in Unconventional Reservoirs." In International Petroleum Technology Conference. IPTC, 2021. http://dx.doi.org/10.2523/iptc-21296-ms.

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Abstract Adsorption isotherms of methane and carbon dioxide adsorption isotherms on shale samples are measured and the simplified local density (SLD) model is used to match the experimental data. The SLD equation also has the generic modeling capabilities to draw the deviation from bulk properties as a function of fluid-solid interaction energies. Still, compared to other available techniques such as molecular simulation, SLD has an advantage of mathematical simplicity and being run at shorter time interval. The model also has satisfactory potential to paint the underlying mechanisms for adsorption preference of one fluid component over the other. The higher adsorption preference of carbon dioxide is reflected in the measurement data and the adsorption model is successfully used to fit the data. In addition to different critical properties of carbon dioxide compared to methane, the main important factor that may describe the high adsorption affinity of carbon dioxide is the fluid-solid potential energy. As shown in this work, the potential energy function shows deeper well depth, the deeper the well depth, the stronger the interaction between the fluid particles and solid surface. By storing gas in high density, liquid like adsorbed phase, the adsorption mechanism can enhance the overall storage capacity of CO2 in deep reservoir rock relative to if there were a free phase alone.
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Mercury, M., M. Carette, A. Janulyte, R. Denoyel, C. Reynard, Y. Zerega, and A. Simon-Masseron. "Experimental study of the zeolite affinity for the dioxin adsorption for a new direct and on-line measurement method." In 2009 1st International Conference on Advancements in Nuclear Instrumentation, Measurement Methods and their Applications (ANIMMA). IEEE, 2009. http://dx.doi.org/10.1109/animma.2009.5503701.

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Messaïkeh, H., N. Belattar, D. Gulino, J. Jozefonvicz, and Y. Sultan. "REMOVAL OF HUMAN ANTIBODIES TO FACTOR VIII :C FROM HEMOPHILIAC PLASMA USING NEW SYNTHETIC SORBENTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644716.

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Human antibodies that neutralize factor VIII procoagulant activity (Anti VIII:C) detected in polytransfused patients with hemophilia A cause serious difficulties for the affected patients as they inactivate any injected FVIII preparations.We hypothesized that FVIII:C might possess active sequences composed of amino acids able to bind Anti VIII:C antibodies. Consequently, completely synthetic resins with suitable chemical substituents mimicking these sequences might interact with Anti VIII:C antibodies. Based upon this hypothesis, crosslinked polystyrene was substituted by various amino acids or their derivatives in order to obtain completely synthetic adsorbents able to remove Anti VIII:C antibodies from hemophiliac plasmas. To establish the relationship between chemical composition of the resins and their affinity towards Anti VIII:C antibodies, the "in vitro" removal of these inhibitors from hemophiliac1s immunoglobulins G was tested by measuring simultaneous adsorptions of either IgG or Anti VIII:C antibodies. Specific and accurate methods veil adapted for studying the adsorption of these two kinds of proteins were used for evaluating either the IgG concentrations (rocket iirrnunoelectrophoresis) or the Anti VIII:C concentrations (immunoradicmetric method). To determine the most suitable chemical groups able to develop a specific adsorption, a first screening on amino acid substituents was undertaken and allowed the selection of glutamic acid and hydro -xyproline. In fact, among the twenty resins tested, the most interesting one is obtained by linking glutamic dimethyl ester derivative onto the polystyrene matrix. This resin possesses a pseudo-specificity towards Anti VIII :C antibodies as it is possible to remove 12 % of Anti VIII :C antibodies and only 3 % of IgG on 5 mg of this resin. Furthermore, the hydrophobic sites on the surface seems to be involved in the Anti VIII :C adsorption as demonstrated by comparing selectivity obtained with mono and diester derivatives either in aspartic or glutamic series.
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Sheveleva, Irina V., Veniamin V. Zheleznov, Svetlana Yu Bratskaya, Valery G. Kuryavyi, and Valentin A. Avramenko. "Adsorption of Cesium Radionuclides by the Composite Sorbents Carbon Fiber/Transition Metals Ferrocyanides." In ASME 2011 14th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2011. http://dx.doi.org/10.1115/icem2011-59255.

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Among various methods of cesium removal from aqueous solutions, sorption using transition metals ferrocyanides is the most efficient method due to extremely high affinity of cesium ions to ferrocyanides. The efficiency of transition metals ferrocyanides application is known to depend on the crystal size being the highest for nanocrystals. Although nanocrystals are difficult to handle in direct application, they can be used in composite materials. In this case two main problems arise: how to control the crystal size of transition metals ferrocyanides and fix them reliably in the supporting matrix. Here we suggest a new route to preparation of composite materials selective to cesium ions using transition metals ferrocyanides stabilized by siloxane-acrylate latexes. The size of transition metals ferrocyanides is controlled by the size of latex particles and their stability is determined by ionization of polyacrylic acid carboxylic groups on the functionalized latex surface. These functionalized particles can be used as precursors in preparation of composite materials by sedimentation and polymerization of latexes on the solid surface of porous matrix, e.g. carbon fibers. Several routes of preparation of carbon fiber based composite materials using functionalized latexes and sorption properties of the obtained materials are discussed. The effect of preparation conditions (method used, carbon fiber polarization potential, concentration of latexes functionalized with transition metals ferrocyanides) on cesium uptake by composite sorbents from solutions of various salinity is reported.
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