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1

Cook, Mandy Lee Hill. "Behavioral and auditory evoked potential (AEP) hearing measurements in odontocete cetaceans." [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001769.

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Utz, Heidi Lockhart. "Collective Identity in Appalachia: Place, Protest and the AEP Power Line." Thesis, Virginia Tech, 2001. http://hdl.handle.net/10919/31926.

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Previously, social movement theory has focused on constructs of identity, such as race/ethnicity, gender and sexual preference, for collective identity construction. Prochansky (1983:59) introduces the concept of place identity, situating it along with the other components of identity, such as the ones mentioned above. In addition, literature on Appalachia has shown land to be an important construct of Appalachian peoples identity. This paper analyzes, through content analysis, the collective identities of writers who wrote letters to the U.S. Forest Service in opposition to a proposed AEP power line. This power line was to run through lands in Appalachia, such as various private properties, the Jefferson and George Washington National Forests, and across the New River. Collective identities based on place-identity, specifically including land, were the main target of analysis, due to the importance of land for Appalachian people. This analysis suggests that land, as a type of place identity, does serve as a basis for collective identity.
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Sugathan, Aromal, and Sean Gregory. "Analysis of AEP prediction against production data of commercial wind turbines in Sweden." Thesis, Högskolan i Halmstad, Akademin för företagande, innovation och hållbarhet, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-44527.

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Based on data from 2083 wind turbines installed in Sweden since 1988, the annual energy production (AEP) predictions considered at the project planning phases of the wind turbines in Sweden have been compared to the wind-index-corrected production data. The production data and the predicted AEP data are taken from Vindstat, a database that collects information directly from wind turbine owners in Sweden. The mean error for all analyzed wind turbines was 11.9%,which means that, overall, the predicted AEP has been overestimated. There has been improved accuracy with time and error in prediction decreasing from 12% to 6.3% for wind turbines installed in the 2000s and 2010s, respectively. However, the overall improvement in accuracy seems to have stagnated around 2005 despite the refinement of forecasting methods and better data availability. From the results analyzed for effects of terrain, the error is smaller for wind turbines in forest areas than in open terrain, indicating that the complexity of forest terrain is not the reason behind the error. Also, there is no apparent increase of error with wind farm size, which could have been expected if the wind farm blockage effect was a primary reason for the overestimations. Comparison between significant wind turbine manufacturers Vestas and Enercon in the Swedish context, the error was more prominent for Enercon.
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Savary, Michael. "Prévision de la turbidité par apprentissage statistique : application au captage AEP d'Yport (Normandie)." Thesis, Normandie, 2018. http://www.theses.fr/2018NORMR091/document.

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Près de 25% de la population mondiale est alimentée par de l’eau en provenance d’aquifères karstiques. La compréhension et la protection de ces derniers apparait donc comme essentielle dans le cadre d’une augmentation des besoins en eau potable. De plus, une contamination des forages d'alimentation en eau potable par une eau turbide peut s'avérer fortement dommageable car entrainant une possible contamination des populations desservies. Dans le cas de la Normandie, des coupures régulières son nécessaires afin de préserver la santé des habitants. La modélisation et la prédiction des augmentations de turbidité apparaissent comme un travail difficile du fait des nombreux phénomènes et paramètres régissant la turbidité ainsi que la non-linéarité de la réponse entre les précipitations et la turbidité. Peu de modèles à l'heure actuelle ont été proposés pour représenter la relation liant la turbidité avec les précipitations. C'est ainsi, en s'intéressant au forage AEP d'Yport responsable de l'alimentation en eau potable de la ville du Havre, que nous proposons une application des réseaux de neurones pour la prévision de la turbidité. Durant les travaux de thèse, nous avons mis en avant la nécessité d'effectuer des campagnes d'échantillonnages des produits phytosanitaires afin de permettre l'identification des éventuels proxies des produits phytosanitaires tel que la turbidité, les précipitations ou bien la conductivité. Par la suite, les travaux effectués dans cette thèse nous ont permis (i) de monter que les modèles par réseaux de neurones permettent de prévoir à 12h et 24h les variations de turbidité, (ii) de tester plusieurs voies d'amélioration de ces modèles, (iii) d'intégrer l'analyse multirésolution aux modèles par réseaux de neurones et pour finir (iiii) d'identifier un semi proxy des contaminations en produits phytosanitaires
Approximately 25% of the world's population is supplied by water from karstic aquifers. The understanding and protection of these appears to be essential in the context of drinking water needs increasing. In addition, contamination of drinking water by turbid water can be highly damaging by resulting in possible contamination of the served populations. In the case of Normandy, regular drinking water cut-off are necessary to preserve the health of the inhabitants. The modeling and prediction of turbidity event appears as a challenging work because of the number of phenomenon and parameters involves in turbidity variation as well as the non-linearity of the link between rainfall and turbidity. Actually, few models have been proposed to represent the relationship between turbidity and rainfall. In this context, by focusing on Yport's pumping well which is responsible for Half of Le Havre city drinking water supply, we propose an application of neural networks for turbidity prediction. During this thesis work, we emphasized the need to carry out sampling campaigns for phytosanitary products to enable the identification of possible phytosanitary product proxies such as turbidity, rainfall or conductivity. Subsequently, the work carried out in this thesis enabled us to (i) designed neural network models allow to predict at 12h and 24h the turbidity variations, (ii) test several ways to improve these models, (iii) integrate multiresolution analysis into neural networks models and finally (iiii) identify a semi proxy for phytosanitary product contamination
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5

Morin, Jacques. "Validation de l'échelle Appropriateness Evaluation Protocol, AEP, en gériatrie et dans les disciplines médicales." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0013/MQ41967.pdf.

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Yoda, Cyrille. "Les enjeux de l'Accord de Partenariat Economique (AEP) entre l'Union européenne et les pays ACP : implications pour l'Afrique de l'Ouest et le Burkina Faso." Thesis, Strasbourg, 2019. http://www.theses.fr/2019STRAB013.

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Les relations commerciales entre l’Union européenne (UE) et les Etats d’Afrique, des Caraïbes et du Pacifique (ACP) ont longtemps été caractérisées par un régime préférentiel non réciproque. Ce régime a finalement été dénoncé en raison de son incompatibilité avec les règles de l’Organisation mondiale du commerce (OMC). Pour se mettre en conformité avec les exigences de l’OMC, l’UE a voulu que ses relations commerciales avec les Etats ACP soient désormais régies par des Accords de partenariat économique (APE) qui doivent être négocié avec les groupements régionaux ACP. Après plus de 12 ans de négociations, les pays de la Communauté économique des États de l'Afrique de l'Ouest (CEDEAO) ont signé les APE avec l’UE ; sur fond de controverses quant aux réels bénéfices attendus d’un tel accord. Aussi, notre thèse s’est proposée de réévaluer l’impact (en termes de création ou de détournement de commerce) des APE sur les économies des pays de l’Afrique de l’Ouest, et plus particulièrement le Burkina Faso. Cette étude est basée sur un modèle d’équilibre général calculable (MEGC) construit à partir du modèle PEP-1t de Decaluwé et al. (2013) et calibré avec la Matrice de Comptabilité sociale (MCS) du Burkina version 2012. Nos résultats montrent que les APE donnent un avantage compétitif à l’UE avec des effets de détournement de commerce qui se traduisent par une augmentation des importations en provenance de l’UE, ce qui contraste avec l’absence d’un effet d’accroissement réel des exportations du Burkina Faso vers l’UE et la CEDEAO. Toutefois, les APE améliorent sensiblement le bien-être des populations, mais ne permet pas de réduire la pauvreté et les inégalités
Economic relations between the European Union (EU) and the African, Caribbean and Pacific Group of States (ACP) have been characterized by non-reciprocal preferential trade agreements. These trade agreements have been continuously criticized for being incompatible with WTO rules. In order to conform to the norms of international trade, the EU has required that its economic relations with the ACP states are governed by the Economic Partnership Agreements (EPA), which are negotiated with three African Regional Economic Communities. After more than twelve years of negotiations, the countries of the Economic Community of West African States (ECOWAS) have signed the EPA with the EU, despite differing opinions on the real benefits that can be expected from such agreements. The aim of this thesis is to evaluate the impact of the EPA on the economies of the ECOWAS (in terms of trade creation and diversion), in particular Burkina Faso. The study uses a modified version of the computable general equilibrium model developed in Decaluwé et al. (2013). The static baseline model is calibrated using a social accounting matrix (SAM) for Burkina Faso’s economy of 2012. Our results show that the APE give the EU a competitive advantage. Burkina Faso’s imports originating from the EU increase, manifesting trade diversion. The trade agreements have no significant effect on Burkina Faso’s exports to the EU or the ECOWAS. Furthermore, while the APE considerably improve population well-being, they do not lower poverty or social inequalities
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Devine, Sinéad. "Exploring exposure to trauma in a sample of adolescents attending alternative education provision (AEP) in Northern Ireland." Thesis, Queen's University Belfast, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.676282.

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The research aimed to increase understanding of the adverse life experiences young people attending AEP have been exposed to. The impact of exposure was captured quantitatively - in relation to PTSD and related symptoms scores ~ and qualitatively by accessing Pupil Voice through semi~structured interviews. Sample: The original sample screened comprised 45 young people (25 males; 20 females) attending AEP. The mean age of participants was 15.82 years. Method: Screening measures were employed to identify young people who had been exposed to potentially traumatic events and whether or not they had been affected by this exposure. A subsample of participants were identified and interviewed in relation to the impact of exposure to trauma. Results: Adolescents in AEP settings have been exposed to a wide range of adverse events and have been significantly impacted as a result. Cognisance was taken of Northern Ireland's post~conflict status in an effort to contextualise the results. Conclusion: Adverse life events can have a widespread impact on the lives of young people, extending beyond clinical levels of PTSD and related disorders. School based educators and professionals are well placed to provide support to trauma exposed youth. Support efforts must holistically consider the wider impact of trauma and its manifestations both within and outside the learning environment
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Ferrer, Tarrés Joan Maria. "Revisión de la utilización en el área de observación de urgencias: validez y fiabilidad de una adaptación específica del protocolo AEP." Doctoral thesis, Universitat de Barcelona, 2003. http://hdl.handle.net/10803/2833.

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Fundamento: Los servicios de urgencia constituyen una de las principales puertas de acceso al sistema sanitario. La preocupación por el aumento de la demanda urgente y por su uso indiscriminado, estimula la necesidad de revisar la utilización y organizar áreas alternativas a la hospitalización convencional.

Objetivo: Evaluar la aplicabilidad específica de los procedimientos de revisión de la utilización en la atención de las urgencias. Diseñar un protocolo AEP adaptado a las áreas de observación de urgencias (AOSU). Cuantificar el porcentaje de admisión y estancias inadecuadas, identificando sus motivos, y las variables que se encuentran asociadas.

Material y método: Diseño de un protocolo adaptado con mínimas modificaciones del AEP original. Se analizó la concordancia en la utilización del instrumento intra e interobservadores que obtuvo índices de kappa del 0,80 y 0,68, respectivamente. Tras un análisis descriptivo, se utilizó el paquete estadístico SPSS que permitió un análisis bivariante, cálculo de odds ratio y análisis de regresión logística múltiple.

Resultados: Estudio concurrente de 4.700 pacientes ingresados consecutivamente en AOSU del Hospital Mútua de Terrassa (Barcelona) durante 18 meses. El 55% de los pacientes eran hombres y la edad media global 63,9 años (+/-20,0). Ingresaron para observación de Medicina (78,5%), Cirugía (11%) y Trauma (9%). La estancia media global fue 29,3 horas (+/-17,5), siendo el 35,5% de las altas a domicilio, y el 62% de los pacientes ingresaron. La proporción de ingresos inadecuados en AOSU representó el 5,5% de las admisiones, debido principalmente al acceso directo del paciente a urgencias, priorización de pruebas y atención que podía prestarse a nivel ambulatorio. Las variables independientes significativamente asociadas con una mayor probabilidad de admisión inadecuada han sido la estación del año (verano, primavera y otoño), presión de urgencias <85%, complejidad de la patología <0,6 (según GRD) y sexo mujer. La proporción de estancias inadecuadas fue del 16,9%, motivadas principalmente por déficit de camas hospitalarias convencionales para el adecuado drenaje de pacientes. Se asoció a la edad >64 años, estación (otoño e invierno), duración de la estancia >24 horas, ingreso hospitalario al alta de AOSU, complejidad >0,6, especialidad de medicina y admisión inadecuada previa.

Conclusiones: La utilización de un servicio de urgencias se ve influenciado por características del enfermo (edad, sexo, complejidad), el modelo organizativo y de gestión (especialidad, estancia, presión de urgencias) además de factores ambientales (estacionalidad, día de la semana, horario) y puede optimizarse si se potencia el funcionamiento protocolizado de áreas de observación previos a la admisión definitiva. La monitorización periódica de la adecuación ha de ser un componente esencial del Plan de Calidad de los centros.
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Colbert, Debborah. "Manatee Sound Localization: Performance Abilities, Interaural Level Cues, and Usage of Auditory Evoked Potential Techniques to Determine Sound Conduction Pathways." [Tampa, Fla] : University of South Florida, 2008. http://purl.fcla.edu/usf/dc/et/SFE0002489.

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Powell, Jessica R. "Depredation and angler interactions involving bottlenose dolphins (Tursiops truncatus) in Sarasota Bay, Florida." [Tampa, Fla] : University of South Florida, 2009. http://purl.fcla.edu/usf/dc/et/SFE0002920.

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11

Sheehan, Jared J. "Risk and CSR Reporting: A Case Study of AEP’s Corporate Accountability Report." Miami University Honors Theses / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1303341741.

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Wang, Jialin. "Building integrated wind energy." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/building-integrated-wind-energy(81978798-e68a-4189-87b0-4159b280b6e9).html.

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In considering methods of reducing the emission of carbon dioxide; there is a growing interest for use of wind power at domestic building in U.K. But the technology of wind turbines development in building environment is more complicated than in open areas. Small wind turbines in suburban areas have been reported as having unsatisfactory energy output, but it is not clear whether this is due to insufficient wind resource or low turbine efficiency. The aim of this research is to discover whether the wind resource in suburban areas is large enough for small wind turbines to produce a useful energy output.Historical wind data and manufacturers' turbine characteristics were used to estimate the hourly wind speed and energy output for different U.K. cities, terrain zones and turbines. It was found that for turbines at 10 m height in suburban areas and depending on city, the annual wind energy conversion efficiency ranged from about 20 to 40%, while the number of turbines required to produce the annual average electricity consumption of a UK dwelling ranged from about 6 for the smallest turbine (5.3 m² rotor area) to about 1 for the largest (35.26 m² rotor area).This analysis was based on average conditions, but the wind speed near buildings can vary considerably from one point to another. In order to predict the performance of wind turbines more accurately, the atmospheric boundary layer (ABL) of suburban areas was simulated in both CFD and wind tunnel models, and models of groups of semi-detached and terraced houses were set in this ABL. It was found that at 10 m height in the area of the houses, the turbulence intensity was too high for satisfactory operation of wind turbines (19 to 35%) while the mean velocity at different points ranged from 86 to 108% of the 10m reference velocity. At 30m height the turbulence intensity was satisfactory (less than 19 %), while the mean velocity ranged from 92 to 103 % of the 30 m reference velocity. It is concluded that for wind turbines in suburban areas, at 10 m height the wind speed is too low and the turbulence is too high for satisfactory performance, while at 30 m height the wind speed is much higher and the turbulence is low enough.
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Limbert, Susanne [Verfasser], and Stefan [Akademischer Betreuer] Kleinschmidt. "Vergleich der Narkosetiefemonitore AEP Monitor /2, BIS Vista und Narcotrend-Compact Version 4.0 unter Target-Controlled Infusion mit Propofol und Sufentanil / Susanne Limbert ; Betreuer: Stefan Kleinschmidt." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2019. http://d-nb.info/1191179052/34.

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Joliffe, Edward Keith, and n/a. "Developing a multiple discourse model of analysis through an evaluation of the National Aboriginal and Torres Strait Islander Education Policy." University of Canberra. Education, 1995. http://erl.canberra.edu.au./public/adt-AUC20060802.170810.

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The overarching research problem for this study was the need to improve upon rational models of policy analysis and delivery, to suit complex postmodern implementation environments. A theoretical model suited to implementing and evaluating major education reform initiatives was devised. Called the 'Multiple Discourse Model', it was grounded in systems theory, containing elements reminiscent of social systems, organisational and structural functionalist research, especially that of Hoy and Miskel (1982)1. However the model was also designed to incorporate a parallel naturalistic analysis reminiscent of postmodern critical pragmatic approaches, such as those explored by Cherryholmes (1994)2. Over a period of five years, this model was developed through an evaluation of the implementation of the National Aboriginal and Torres Strait Islander Education Policy (AEP) in the Australian Capital Territory government secondary schools sector. The distinguishing feature of the study's methodology was its multiperspective analysis, an approach suggested by Mclaughlin (1987)3 to take account of the differing communities of discourse which exist in a reformist policy implementation environment. To operationalise the research problem, dimensions of policy effectiveness were articulated. These were addressed through a comprehensive set of research indicators, extracted from the AEP's national policy goals and the local strategic and operational plans. Data aimed at judging the effectiveness of implementation were collected from multiple sources using multiple research instruments. These data were analysed in three stages using a purpose-designed computer program which could cross-reference between the four interacting dimensions of research indicators, research instruments, data sources, and potential variables modifying policy/program outcomes. It was found that this model produced clear conclusions about the effectiveness of AEP implementation in the delimited sector, within the framework of the AEP's own policy assumptions. The model also provided insights into critical issues which are generalisable to the national context, such as the power of cultural hegemony and the socio-political predicament of Aboriginal and Torres Strait Islander dispossession. As a methodology, the model was found to have a number of technical advantages, including its capacity for focussing on selected areas of the implementation environment, its provision of access to multiple levels of detail amongst data and its possession of mechanisms for monitoring its own internal validity. The evaluation case study, used as the vehicle for the Multiple Discourse Model's development, demonstrated that best-practice administration was in place which enhanced short and medium-term policy/program outcomes. However, the study's findings also suggested that a fundamental disjuncture existed between the AEP's policy/administration paradigm and the conflicting assumptions of the primary target communities, reinforcing the findings of Sykes (1986)4. The research results suggested that despite measurable successful inputs, the planned long-term outcomes of the AEP will not necessarily be achieved. No significant administrative structures or actions were apparent which could resolve this lack of synchrony at the interface between government delivery systems and 'grass roots' Aboriginal and Torres Strait Islander community values. This raised doubts about whether any systems approach, however well refined, could be socially useful not only for evaluation, but also as a basis for reform policy and public administration in a postmodern pluralist democratic setting. The evaluation was therefore used as a locus for theoretical reflection as well. A new policy paradigm is suggested, based on a power-sharing 'theory of community', more in keeping with Aboriginal and Torres Strait Islander peoples' aspirations for self determination and more likely to alleviate the so far unresolved destructive effects of cultural and political dispossession.
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Elangovan, Saravanan. "The Role of Auditory Event Related Potentials in Understanding Speech Perception." Digital Commons @ East Tennessee State University, 2006. https://dc.etsu.edu/etsu-works/1578.

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Gelotte, Lovisa, and Nilsson Alexandra Lundevall. "Optimal Placement of FloatingTwo-Turbine Foundations in Offshore Wind Farms." Thesis, KTH, Energiteknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-209833.

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This project is conducted in cooperation with Hexicon AB, which is a Swedish design and engineering company developing floating two-turbine platforms for offshore wind power.The study aims to investigate the optimal placement of Hexicon AB’s platforms in an offshore wind farm with respect to the Annual Energy Production (AEP). Wind farm layout optimization is a complex problem and it has been approached by the development of calculation and optimization programs in Matlab. The analytical Jensen wake model has been utilized for calculation of AEP and important inputs to the program have been turbine parameters and site specific conditions. The optimization strategy used is a multi-stage algorithm where the gradient-based local search algorithm Fmincon has been used in combination with a version of the heuristic genetic algorithm. The developed programs have been tested and evaluated through a case study. Included in the case study is also a brief financial evaluation regarding how different scenarios in electricity export price and costs for cabling could affect the feasibility of the optimized layouts. Concluded from the project is that the developed programs can be used to investigate the optimal placement of floating two-turbine platforms with respect to AEP. In the case study it was found that the optimized layout obtained a wind farm efficiency of around 4% more than for the conventional staggered layout that was tested. What is also emphasized is that the feasibility of the optimized layouts obtained from the program is quite sensitive toward changes in future electricity export price and costs for cabling and installation. Hence, it is important to perform a careful financial analysis in order to draw conclusions regarding what layout is the better option for a specific situation.
Dagens utbyggnad av vindkraft sker i allt större utsträckning genom etablering av vindkraftparker. De främsta fördelarna med att placera vindkraftverken i parker är att de höga fasta kostnaderna fördelas på flera kraftverk samt att man kan beställa ett flertal enheter samtidigt och därigenom minska kostnaden per installerad Megawatt (MW). För att ytterligare kunna öka vinsten på investeringen är det viktigt att undersöka optimal inbördes placering av vindkraftverken för att erhålla en så hög energiproduktion som möjligt. Det finns många studier gjorda inom området och ett flertal programvaror utvecklade. Dock finns det endast ett fåtal studier som har inriktat sig specifikt på flytande vindkraftverk. Detta arbete är utfört i samarbete med Hexicon AB, vilket är ett Stockholmsbaserat ingenjörsföretag som utvecklar en patenterad teknik för plattformar avsedda för flytande vindkraft. Det unika med Hexicon ABs patenterade teknik är att två vindkraftverk är placerade på en gemensam plattform. Denna teknik gör det möjligt för plattformen att anpassa sig till vindriktningen vilket ger en ökning av kraftverkens energiutbyte. Då det inte finns några utvecklade optimeringsmetoder för flytande plattformar som kan anpassa sig efter vindriktning är syftet för denna studie att undersöka den optimala inbördes placeringen av Hexicon ABs plattformar i en vindkraftpark. Eftersom vindkraftsoptimering är ett komplicerat problem som bland annat är icke-linjärt och icke-konvext så finns det ingen exakt lösning tillgänglig för problemet. Komplexiteten gör även många förenklingar och antaganden nödvändiga för att kunna bearbeta problemet. I detta projekt har sambandet mellan årlig elproduktion och inbördes placering av plattformarna undersökts genom att ett kalkylerings- och optimeringsprogram utvecklats i programvaran Matlab. För att kunna undersöka den optimala inbördes placeringen av vindkraftverken är det viktigt att förstå hur vindkraftverken påverkas av att placeras tillsammans i en park. För att göra detta så behövs en modell för att beskriva den så kallade vaken som uppstår bakom respektive vindkraftverk. Detta gjordes genom att använda den analytiska Jensen vakmodellen, vilket är den vanligaste modellen att använda för optimeringssyften. Beräkningen av elproduktion gjordes baserat på given information angående turbinparametrar samt specifika förhållanden på platsen för vindparken. För det utvecklade optimeringsprogrammet användes en tvåstegsalgoritm där den gradientbaserade algoritmen Fmincon utgjorde den centrala delen. Fmincon är en effektiv algoritm för lokal optimering som finns tillgänglig i Matlab. För att generera bra startgissningar till den lokala optimeringen användes en version av en heuristisk genetisk algoritm som komplement till Fmincon. Denna algoritm bygger på samma princip som processen för naturligt urval i evolutionssammanhang där de bäst lämpade individerna för vidare sina egenskaper till nästa generation. För att ytterligare förbättra algoritmen kompletterades den även med ett moment av slumpmässighet. För att testa och utvärdera de utvecklade programmen genomfördes en fallstudie. I denna studie optimerades 50 stycken olika heuristiska startgissningar. De 20 bäst presterande konfigurationerna valdes ut för vidare analys där de blev utvärderade med avseende på olika scenarion för elpris samt kostnad för elektrisk infrastruktur. Detta för att undersöka hur den optima placeringen eventuellt skulle kunna påverkas av osäkerheter i dessa faktorer. Den genomförda fallstudien indikerade att de utvecklade programmen kan användas för att undersöka den inbördes optimala placeringen av vindkraftverk med avseende på elproduktion. Den ekonomiska utvärderingen indikerade även att den optimala placeringen var känslig för olika scenarion där elpris och kostnader för infrastruktur varierades och att detta kunde påverka lönsamheten för investeringen. Det ska därför betonas att det anses vara viktigt att utföra en mer noggrann ekonomisk utvärdering av de optimerade konfigurationerna för att undersöka vilken positionering som är mest lämplig för en viss situation.
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Kempf, Lisa Katharina Verfasser], Jens [Akademischer Betreuer] Lehmberg, and Henning [Akademischer Betreuer] [Bier. "Nicht-invasive Elektrocochleographie mit einer Tympanon-Spiralfeder-Elektrode zum intraoperativen Monitoring der Hörfunktion : Eine alternative Ableitung eines AEP / Lisa Katharina Kempf. Betreuer: Jens Lehmberg. Gutachter: Henning August Bier ; Jens Lehmberg." München : Universitätsbibliothek der TU München, 2016. http://d-nb.info/1088724930/34.

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Nikjeh, Dee Adams. "Vocal and instrumental musicians : electrophysiologic and psychoacoustic analysis of pitch discrimination and production." [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001728.

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Greenhow, Danielle. "Hearing and Echolocation in Stranded and Captive Odontocete Cetaceans." Scholar Commons, 2013. http://scholarcommons.usf.edu/etd/4682.

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Odontocetes use echolocation to detect, track, and discriminate their prey, as well as negotiate their environment. Their hearing abilities match the frequency of greatest sensitivity to the higher frequencies used for foraging and navigation. Hearing and echolocation together provide odontocetes with a highly developed biosonar system. This dissertation examines the hearing ability of several odontocete species to understand what signals they can perceive during echolocation. The variability in hearing ranges between species is examined in the context of phylogenetic and ecological differences among taxa. An autonomous hydrophone array is also developed that could be used in an expanded form in field deployments to study echolocation signals in a wider range of species. Methods for measuring hearing sensitivity include both psychophysical and electrophysiological procedures. Behavioral methods require a large time commitment, for both training and data collection, and can only be performed on captive dolphins. Auditory evoked potential (AEP) methods are non-invasive, rapid measurements of the brain's response to sound stimuli and allow for audiograms to be collected on stranded, high risk dolphins. By determining the hearing abilities of odontocetes either in captivity or during stranding, data can be collected about inter- and intraspecies variability, and the occurrence of hearing impairment. It can also be used as another diagnostic tool to determine the releasability of a stranded animal. A juvenile male short-finned pilot whale (Globicephala macrorhynchus) that stranded in Curacao had severe hearing impairment at all frequencies tested. Four female short-finned pilot whales tested had the best sensitivity at 40 kHz. The juveniles had greater high frequency sensitivity than the adult pilot whales. Cutoff frequencies were between 80 and 120 kHz. Hearing sensitivity was determined for the two mother/calf pairs of Risso's dolphins (Grampus griseus) before and after antibiotic treatment in order to measure any potential effects of antibiotic treatment. Greatest sensitivity occurred at 40 kHz and cutoff frequencies were around 120 kHz for all dolphins tested. Changes in hearing sensitivity after antibiotic dosage were 12 dB or less in all cases except one. The adult female Betty showed a threshold shift at 120 kHz of 54 dB from May to June, which partially demonstrates the presence of an ototoxic effect at one frequency. Dosages of antibiotics during drug treatment detailed in this study should be considered safe dosages of antibiotics for Risso's dolphins. AEP and behavioral methods were used to collect audiograms for three Stenella spp. dolphins. The frequency of best hearing for the Atlantic spotted dolphin and the spinner dolphin was 40 kHz, and their upper cutoff frequencies were above 120 kHz. The pantropical spotted dolphin had the greatest sensitivity at 10 kHz, and had severe high frequency hearing loss with a cutoff frequency between 14 and 20 kHz. Comparisons of high frequency hearing sensitivities among the species tested show two distinct groups. Short-finned pilot whales and Risso's dolphins have a cutoff frequency below 120 kHz, whereas Stenella spp. dolphins have cutoff frequencies above 120 kHz. Expanding the comparison to include other species, killer whales, pygmy killer whales, false killer whales, and long-finned pilot whales also have cutoff frequencies below 120 kHz. Common bottlenose dolphins, white-beaked dolphins, Indo-Pacific humpback dolphins, rough-toothed dolphins, and common dolphins have cutoff frequencies above 120 kHz. Genetic evidence exists for two subfamilies within Delphinidae (Vilstrup et al., 2011) and those species with cutoff frequencies below 120 kHz belong to the subfamily Globicephalinae and those species with cutoff frequencies above 120 kHz belong to the subfamily Delphininae. An autonomous, field-deployable hydrophone array was developed to measure free-swimming echolocation. The array contained 25 hydrophones, two cameras, and a synchronization unit on a PVC frame. The distinct click train was used to time-align all 25 channels, and the light was used to synchronize the video and acoustic recordings. Echolocation beam patterns were calculated and preliminary evidence shows a free-swimming dolphin utilizes head movement, beam steering and beam focusing. Among all areas of cetacean biology more research is necessary to gain a clearer picture of how odontocetes have adapted to function in their acoustic environment. The array system developed can be used to study how dolphins use echolocation in the wild, the impacts of anthropogenic sound on echolocation production, and the potential consequences of high frequency hearing loss.
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20

Aniceto, Erica Alessandra Fernandes. "A linguagem da periferia: construção de identidade por alunos de EJA de uma escola pública." Universidade Federal de Viçosa, 2011. http://locus.ufv.br/handle/123456789/4842.

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Starting from the premise that language is a form of social action, the main aim of this study is to examine how students of an Adults Education Program (AEP), from a public school located in a peripheral neighborhood of a city in Minas Gerais, build and take their social identities. Grounded in theories of Ethnomethodological Conversation Analysis (ECA) and in Interactional Sociolinguistics (IS), we analyze the linguistic and discursive choices of these students, observed from the talk-in-interaction in a focus groups, to identify how these students demonstrate membership in certain social categories. To collect the data, we used a focus group, through which students narrated their experiences of living in a peripheral neighborhood. After a detailed transcript of the data generated in the meetings with students, which was done according to the proposal from ECA, we analyzed it based on the concept of Sacks‟ Membership categorization (1992). Since the categorization processes are in use and in constant negotiations, we revealed, thus, students' discursive action, during interactions with one another, to build their identities through self-categorization and categorization. The results of this research indicate participants‟ orientation throughout the interaction, building, through speech, the identity of outsiders, ratifying the label assigned to them by those who occupy prestigious positions of power and by those who recognize themselves as the "good society", as Elias e Scotson (2000). By categorizing themselves as deviant, the participants seem to perceive their place in the city surveyed, but, they do not often accept this label passively: this generates reports of a conflictual relationship between these young people and the members of the so-called "good society". Thus, we conclude that through membership categorization, students build and assume the identity of outsiders, and that a description and categorization work is quite relevant to the study of the construction of social identities.
Partindo da premissa básica de que a linguagem é uma forma de ação social, o objetivo central deste estudo é analisar como alunos da Educação de Jovens e Adultos (EJA) de uma escola pública localizada em um bairro periférico de uma cidade do interior de Minas Gerais constroem e assumem as suas identidades sociais. Embasados em teorias da Análise da Conversa Etnometodológica (ACE) e da Sociolinguística Interacional (SI), analisamos as escolhas linguístico-discursivas desses alunos, observadas a partir da fala-em-interação em grupos focais, para verificar como esses estudantes demonstram pertencimento a determinadas categorias sociais. Para coleta de dados, utilizamos a técnica do grupo focal, através da qual os discentes narram a experiência de morar em um bairro periférico. Após uma minuciosa transcrição dos dados gerados nos encontros com os estudantes, feita de acordo com a proposta da ACE, fizemos uma análise baseada no conceito de Categorização de Membros, de Sacks (1992). Uma vez que os processos de categorização estão em uso e negociações constantes, revelamos, assim, a ação discursiva dos estudantes, durante interações entre si, para a construção de suas identidades por meio de autocategorização e categorização. Os resultados desta pesquisa apontam a orientação dos participantes durante a interação, os quais constroem, através da fala, a identidade de outsiders, ratificando o rótulo que lhes é atribuído por aqueles que ocupam posições de prestígio e poder e que se reconhecem como a boa sociedade , os quais chamaremos de estabelecidos, assim como Elias e Scotson (2000). Ao se categorizarem como desviantes, os estudantes de EJA demonstram perceber o lugar deles na cidade pesquisada, mas, muitas vezes, não aceitam passivamente tal rótulo, o que gera relatos de uma relação conflituosa entre esses jovens e os estabelecidos. Assim, ao concluir que, através da categorização de membros, os estudantes constroem e assumem a identidade de outsiders, demonstramos que o trabalho de descrição e de categorização de membros é bastante pertinente para o estudo da construção de identidades sociais.
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21

Kritzinger, Mieke. "Cortical Auditory Evoked Potential (CAEP) and the chirp Auditory Steady State Response (ASSR) in predicting behavioural hearing thresholds in adults with sensorineural hearing loss." Diss., University of Pretoria, 2019. http://hdl.handle.net/2263/73231.

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Abstract Purpose: To compare the frequency specific Cortical Auditory Evoked Potential (CAEP) and the chirp-evoked 40 Hz Auditory Steady State Response (ASSR) with equivalent residual noise levels for behavioural threshold prediction in adults with normal hearing and with SNHL. Method: The study tested 23 adults with normal hearing and 20 adults with SNHL. The participants were aged between 18–65 years. A repeated measures within- participant descriptive design was used to collect the quantitative data. The participants underwent behavioural pure tone, CAEP and ASSR testing on the same day. Results: Similar CAEP difference scores across frequencies for the participants with normal hearing (mean=12.32-14.40 dB) and with SNHL (mean=10.00-16.47 dB) were measured. However, for the ASSR difference scores across frequencies slightly smaller difference scores were measured for the participants with SNHL (mean=10.17-17.30 dB) than for the participants with normal hearing (mean=11.74- 17.14 dB). CAEP thresholds were significantly closer to the behavioural pure tone thresholds at 500 (p=0.028; mean absolute difference 14.40 dB) and 2000 (p=0.016; mean absolute difference 12.56 dB) Hz for participants with normal hearing. In participants with sensorineural hearing loss, CAEP and ASSR thresholds were measured at similar sensation levels and were not statistically different (p>0.05). Conclusion: For the purpose of threshold estimation, representing the auditory function to the level of the auditory cortex the CAEP was closer to the behavioural hearing thresholds than the 40 Hz ASSR at all frequencies except at 4000 Hz, regardless of the hearing sensitivity. Keywords: Auditory steady state response (ASSR), Cortical auditory evoked potential (CAEP), Auditory evoked potential (AEP), Residual noise, Signal to noise ratio (SNR), objective threshold estimation, awake adults.
Dissertation (MA (Audiology))--University of Pretoria, 2019.
Speech-Language Pathology and Audiology
MA Audiology
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22

Cuvenhaus, Annelie [Verfasser]. "ADP induziert ein mitochondrial reguliertes, nukleäres Calcium Signal in AEZ Typ II / Annelie Cuvenhaus." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2020. http://d-nb.info/1232407844/34.

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23

Kristou, Nebil. "Étude et conception de métamatériaux accordables pour la miniaturisation d’antennes aux fréquences micro-ondes." Thesis, Rennes 1, 2018. http://www.theses.fr/2018REN1S016/document.

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Les antennes présentes dans la plupart des systèmes communicants comme les véhicules automobiles, les avions et les trains se multiplient et sont soumises à une contrainte d’intégration de plus en plus sévère. De nombreuses techniques de miniaturisation d’antennes existent et passent toutes par un compromis entre la taille et les performances (bande passante et/ou rendement de rayonnement). Pour les systèmes cités ci-dessus, les antennes sont souvent placées devant ou à proximité d’un réflecteur métallique (toit de véhicule, carlingue d’aéronef). Dans ce cas, l’épaisseur de système antennaire est une contrainte majeure et les métamatériaux de type Conducteur Magnétique Artificiel (CMA) ouvrent des perspectives intéressantes grâce à leurs propriétés électromagnétiques non conventionnelles. Cependant, pour les applications sub-GHz (RFID, LTE, PMR…), les CMA sont limités par les dimensions des cellules unitaires nécessaires à leur mise en œuvre (λg/4) ainsi que leur bande réduite de fonctionnement. Réduire leurs dimensions permet de rendre leur utilisation compatible avec le contexte des antennes miniatures intégrées. Ajouter l’agilité fréquentielle permet de palier le problème de la bande passante réduite dans le cas des antennes et des CMA miniaturisés en ajustant le fonctionnement du système antennaire sur une large bande passante. Cette thèse de doctorat propose d’étudier et de développer un nouveau système antennaire à faible profil composé d’une antenne miniature associée à une métasurface compacte reconfigurable en fréquence et compatible avec le standard NB-IoT dans la bande basse LTE (700 MHz – 960 MHz)
Antennas are now very integrated in several connected systems like cars, airplanes and trains. Many antenna miniaturization techniques exist and all go through a compromise between size and performance (bandwidth and/or radiation efficiency). For the systems mentioned above, the antennas are often placed near a metallic reflector (vehicle roof, aircraft cabin). Within this context, Artificial Magnetic Conductors (AMC) present an attractive reflector for low profile antennas which can take advantage of intrinsic zero reflection phase response to boost antenna performance without the need for thick quarter wave backplane. However, for sub-GHz applications (RFID, LTE, PMR ...), AMC are limited by the size of the unit cells necessary for their implementation (λg/4) as well as their reduced operating bandwidth. AMC miniaturization makes their use compatible with small antennas. Adding tunability restores the possibility of adjusting the operating frequency over a large bandwidth. This PhD thesis proposes to study and develop a new electrically small, low-profile antenna based on miniaturized and tunable AMC for the NB-IoT standard in low LTE band (700 MHz – 960 MHz)
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24

Boyacioglu, Beril. "Microstructural Characterization Of Hypoeutectoid Steels Quenched From The Ae1 - Ae3 Intercritical Temperature Range By Magnetic Barkhausen Noise Technique." Master's thesis, METU, 2006. http://etd.lib.metu.edu.tr/upload/12606921/index.pdf.

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This thesis aims to examine the possibility of using Magnetic Barkhausen Noise technique in characterizing the ferritic-martensitic microstructure of hypoeutectoid steels quenched from the intercritical temperature range. For this purpose, rectangular specimens were prepared from SAE 1020, 1040 and 1060 steels. The specimens were heated at different temperatures within the intercritical temperature range and then quenched into water. Microstructures of the specimens were characterized by metallographic examinations and hardness measurements. The measurements of the Magnetic Barkhausen Noise (MBN) were performed by using both Rollscan and &
#956
SCAN sensor connectors. It was seen that, for specimens having identical carbon content, Barkhausen emission decreased as the heating temperature increased. Moreover, in specimens heated at the same temperature, Barkhausen emission decreased as the carbon content of the specimen increased. In both cases, the decrease in Barkhausen emission is associated with the increase in martensite content. The results indicate that MBN is inversely proportional to hardness and that MBN is very sensitive to the microstructural condition of the material. It has been shown that using MBN is a powerful tool for evaluating the microstructure of hypoeutectoid steels quenched from the intercritical temperature range and that the use of this technique could be extended to characterize industrial dual phase steels.
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25

Bellier, Ludovic. "Encodage neuronal des sons de parole : développements méthodologiques, générateurs neuronaux et application au malentendant appareillé." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10135/document.

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A ce jour, six millions de français sont atteints de troubles de l'audition. Face à ce problème de santé publique, des outils performants d'exploration de la fonction auditive sont indispensables. La Speech ABR (Speech Auditory Brainstem Response ou Réponse du tronc cérébral auditif à la parole) est un outil prometteur, comme marqueur électrophysiologique fin de l'encodage neuronal de la parole. Cependant, sa méthodologie reste peu développée, son origine neuronale incertaine et elle n'a jamais été enregistrée chez le malentendant porteur d'aides auditives. Le premier axe de cette thèse porte sur les générateurs neuronaux de la Speech ABR. Le développement d'une méthodologie de recueil topographique de cette réponse jusqu'alors décrite comme strictement sous-corticale, a d'abord suggéré la possibilité d'un générateur cortical. Une étude en stéréo-électroencéphalographie a ensuite confirmé l'existence d'une activité Speech ABR dans les cortex auditifs primaires bilatéraux. Ce résultat apporte un éclairage nouveau sur la représentation des sons de parole par système nerveux auditif. Le second axe concerne l'étude de la Speech ABR chez le malentendant appareillé. Après avoir développé une méthodologie de stimulation acoustique directement au travers des aides auditives, nous avons étudié la plasticité neuronale induite par le port d'aides auditives. Les résultats montrent une amélioration de l'identification des phonèmes amplifiés, liée à une représentation corticale modifiée et à un encodage fréquentiel rééquilibré. Ces toutes premières preuves de plasticité neuronales dès les 4 premiers mois d'utilisation des aides auditives ouvrent de nouveaux espoirs thérapeutiques
To date, six million French are hearing impaired. To address this public health issue, efficient tools for exploration of the hearing function are essentials. Speech ABR (Speech Auditory Brainstem Response) is a promising tool, being a fine electrophysiological marker of the neuronal encoding of speech. Though, its methodology remains underdeveloped, its neural origin is still uncertain, and it has never been recorded in hearing aid users. The first axis of this thesis focuses on the neural generators of Speech ABR. The development of a methodology for recording topographies of this response, up to now described as strictly subcortical, first suggested the possibility of a cortical generator. A stereo-electroencephalography study then confirmed the existence of Speech ABR activity in bilateral primary auditory cortices. This result sheds a new light on the representation of speech sounds within the auditory nervous system. The second axis concerns the study of Speech ABR in hearing aid users. After having developed a methodology of acoustic stimulation directly through hearing aids, we investigated neural plasticity induced by hearing aid use. Results show an improvement in the identification of amplified phonemes, linked to an altered cortical representation and a rebalanced frequency encoding. This very first evidence of neural plasticity as soon as the first four months of hearing aid use opens up new therapeutic hopes
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26

Wyant, Timothy Lewis 1965. "The generation of a murine mesangial cell line deficient in AE2 expression: Introduction of human AE1 into a murine cell line." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/290615.

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Senescent cell antigen (SCA) is an antigen that was first identified on human erythrocytes. The formation of SCA is a normal cellular process which marks cells as old and/or damaged. This has been clearly demonstrated in the erythrocyte systems and is believed to occur in other cell types. SCA is formed from the breakdown of the major anion transport protein (Anion Exchanger, AE, or band 3). The mechanism for the formation of SCA is currently not understood. Research into the formation of SCA has been hampered by lack of in vitro models that allow the manipulation of the AE protein. Here, we describe the formation of two cell lines and a cell clone which may assist in the elucidation of the mechanisms of SCA formation by allowing AE manipulation. The murine mesangial Mes-13 cell line was chosen as the parental cell line. Initially, it was determined that Mes-13 cells express the Anion Exchanger 2 (AE2) form of AE. A cell line deficient in the expression of the AE2 protein was generated using an AE2 RNA antisense construct transfected into the Mes-13 cells. The expression of the AE2 protein was reduced by greater than 60 percent in one of the cell clones (A-8). A second cell expressing human AE1 was generated. Human AE1 was cloned using polymerase chain reaction from reticulocyte cDNA and placed into an expression vector. This vector, called Repbd3, was transfected into the Mes-13 cells and the A-8 clone. A 90 kD protein and a 45 kD protein reactive with anti-AE1 antibodies were shown to be expressed in the Mes-13 cells transfected with the Repbd3 construct. Only the 45 kD version was observed in a pooled A-8 cell line. In individually cloned A-8 cells transfected with the Repbd3 vector, expression of the 45 kD and 90 kD proteins were observed at low levels. The cells lines and vectors generated in this work will greatly assist in the understanding of the AE proteins, the elucidation of the mechanisms involved in the formation of SCA, and set up a system to determine residues critical for SCA formation by site-directed mutagenesis of human AE1.
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Almeida, Deniz Pedrozo de. "AEO." Florianópolis, SC, 2002. http://repositorio.ufsc.br/xmlui/handle/123456789/82328.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Ciência da Computação.
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Casas, Weisser Daniel Alejandro. "Presencia y distribución de amelogenina, citoqueratina AE1/AE3 y citoqueratina 14 en tumores odontogénicos, dientes con amelogénesis imperfecta hipocalcificada y gémenes denatrios." Tesis, Universidad de Chile, 2010. http://repositorio.uchile.cl/handle/2250/134115.

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Trabajo de Investigación Requisito para optar al Título de Cirujano Dentista
Autor no autoriza el acceso a texto completo de su documento
Introducción: La odontogénesis es un proceso complejo, secuencial y altamente regulado en el que se forman estructuras calcificadas. Alteraciones en este proceso podrían dar lugar a diferentes tipos de enfermedades como alteraciones en el número de dientes y en la formación de esmalte, dentina y cemento. Otras lesiones que se pueden generar a partir de los tejidos remanentes de la odontogénesis son los tumores odontogénicos que derivan de tejido epitelial, ectomesenquimático y/o mesequimático presentando algunos de ellos matrices calcificadas. El objetivo del presente trabajo fue determinar la presencia y distribución de amelogenina, citoqueratina AE1/AE3 (pan citoqueratina) y citoqueratina 14 en tumores odontogénicos, dientes con amelogénesis imperfecta hipocalcificada y gérmenes dentarios. Materiales y métodos: Con técnicas inmunohistoquímica se examinó la presencia y distribución de amelogenina, citoqueratina AE1/AE3 y citoqueratina 14 en: 11 odontomas, 11 tumores odontogénicos adenomatoides (TOA), tres tumores odontogénicos quístico calcificante (TOQC), 2 tumores odontogénicos epitelial calcificante (TOEC), 2 dientes con amelogénesis imperfecta hipocalcificada (AIHC) y 3 gérmenes dentarios en desarrollo. Los tumores odontogénicos fueron obtenidos del Instituto de Referencia de Patología Oral (IREPO) de la Facultad de Odontología de la Universidad de Chile entre los años 1988 y 2008. Resultados: Se detectó amelogenina en la matriz de esmalte de odontomas, de dientes con AIHC y de gérmenes dentarios. Esto también fue observado en matrices calcificadas rodeadas por epitelio en TOA y TOQC y en células fantasmas presentes en odontomas y TOQC. Las citoqueratinas fueron detectadas en las células epiteliales de gérmenes dentarios y tumores odontogénicos estudiados. No se observó la presencia de citoqueratinas en dientes con AIHC. Conclusiones: Las proteínas amelogenina, citoqueratina AE1/AE3 y citoqueratina 14 son identificables a través de técnicas inmunohistoquímicas en algunos tumores odontogénicos que forman matrices tipo esmalte originadas por epitelio odontogénico y en gérmenes dentarios en desarrollo
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Arellano, V. Marcelo, and Godoy Gino Marcelo Bazo. "AES Water." Tesis, Universidad de Chile, 2016. http://repositorio.uchile.cl/handle/2250/144403.

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TESIS PARA OPTAR AL GRADO DE MAGÍSTER EN ADMINISTRACIÓN
Marcelo Arellano V. [Parte I], Gino Marcelo Bazo Godoy [Parte II]
Las empresas relacionadas con el mercado del agua son diversas. Este vital elemento para la vida humana como para los procesos industriales es cada vez más escaso y requiere generar conciencia, compromiso e innovación en usar nuevas tecnologías disponibles para optimizar su uso. Llamaremos AESWater a quien será una empresa que se dedique a la comercialización de agua Industrial en el norte de Chile, aprovechando la plataforma de la central térmica de la zona de Mejillones. Su actividad será generar agua industrial, mediante el proceso de desalinización, particularmente a procesos mineros de la zona, de manera que puedan realizar sus actividades actuales y futuras sin el problema de abastecimiento de este vital elemento, cumpliendo con la nueva normativa, contribuyendo de manera significativa a iniciativas gubernamentales de la gestión del agua y cuidado del medio ambiente. Además, atenderá a clientes tales como, sanitarias y centrales térmicas, quienes necesitan de éste vital elemento para sus actividades. Este modelo de negocio es expandible a los otros sitios donde exista una central térmica costera. El tamaño de mercado potencial es de casi MMUS$1.6041 y en donde la industria de la “generación de agua industrial” es atractiva dada la poca presencia de esta tecnología a nivel nacional, existen pocos sustitutos, las barreras de entrada son altas, la competitividad en el mercado chileno es baja y existen restricciones gubernamentales, por ejemplo de la DGA2, a otorgar nuevos permisos. Referente a los clientes, éstos se identifican en diferentes mercados, lo cual reduce el riesgo debido a la diversificación. Los mercados objetivo detectados son Minería MMUS$ 383, Sanitarias MMUS$ 96 e Industria de la Energía MMUS$ 48 quienes poseen diferentes características por lo cual la estrategia de comercialización a utilizar para cada uno difiere dada sus necesidades. Por ejemplo en normativa, RSE3, en imagen, montos de gasto, etc. Evaluados estos segmentos por atractivo, dan como resultado que los primeros clientes a desarrollar están en los segmentos Minería y luego Sanitarias, la Industrial de la Energía requiere una calidad diferente pero alcanzable como parte adicional de proceso. Una de las actividades clave del negocio son los permisos ambientales vigentes, otro no menor es el costo de la energía dada la sinergia con la termoeléctrica, las capacidades operativas que en conjunto logran la oportunidad en la entrega y adaptabilidad a las necesidades de cada cliente. Esto sumado a un servicio post venta de seguimiento, dado que el relacionamiento con el cliente es (B2B) face to face es muy estrecho. En lo medio ambiental el valor en el negocio propuesto aporta con la disponibilidad del recurso hídrico en lugares alejados. Se busca ofrecer soluciones completas con mínimo impacto ecológico y utilizando energía a bajo costo. La oportunidad comercial es de una ventana limitada con contratos a largo plazo antes de la llegada de nuevos competidores, por lo que el ingreso es agresivo y rápido, apuntando a cubrir el mercado detectado en un plazo de 4 años. Los principales resultados obtenidos en la evaluación económica de este plan de negocio, son una VAN de MMUS$ 556,1, una TIR de 31,86%, con una Inversión Total (Activo + Capital de Trabajo) MMUS$ 410,31 y un Payback de 6 años.
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30

Wagner, Michael J. "AEW aircraft design." Thesis, Monterey, California. Naval Postgraduate School, 1992. http://hdl.handle.net/10945/23815.

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31

Diebel, Nadja. "Akustisch evozierte Hirnstammpotentiale (AEHP) bei Frühgeborenen "Zeitgang-BERA" und konventionelle AEHP /." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=969698712.

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32

Gleiss, Dagmar. "Geschichte der Arbeitsgemeinschaft Evangelikaler Missionen, AEM 25 Jahre AEM, 1969-1994 /." Theological Research Exchange Network (TREN), 1994. http://www.tren.com.

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33

Kopec, David M., and Jeff J. Gilbert. "AEF 130360 Overseeding Safety." College of Agriculture and Life Sciences, University of Arizona (Tucson, AZ), 2004. http://hdl.handle.net/10150/216558.

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Several items need consideration when products are evaluated for use as transition agents. These are (1) efficacy of ryegrass removal [rate of ryegrass decline and appearance of turf during transition], (2) tolerance and performance of incoming bermudagrass and (3) application safety for next season’s repeat overseed operations. This test was designed to evaluate application safety for the next overseeding which occurs in the early fall. Therefore, AEF was applied in the summer to bermudagrass turf prior to overseed operations. Most responses of the perennial ryegrass (overseed) turf to previous treatments of AEF 130360 occurred immediately after overseeding, from mid-October to early November. AEF 130360 applied 2 weeks before overseeding caused a significant decrease in seedling vigor, percent plot ryegrass cover, and percent bermudagrass plot straw present. Both AEF treatments applied closest to overseeding (2 weeks prior) had the least amount of initial ryegrass, the greatest amount of green bermudagrass, and later in the season, the most amount of straw (dormant) bermudagrass. Differences in turfgrass quality were not significant due to treatments at any time throughout the test, and most treatments ranked higher than the control in overall quality. Under the conditions of this test, application of AEF 130360 at either 0.64 or 1.28 ounces/product/M made one month before actual oversseding did not cause detrimental effects to ryegrass emergence, ryegrass cover, turfgrass color or overall quality.
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34

Vitolo, Giancarlo, and Balucani Daniel Alejandro Avila. "Valoración AES Panamá SRL." Tesis, Universidad de Chile, 2018. http://repositorio.uchile.cl/handle/2250/165794.

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TESIS PARA OPTAR AL GRADO DE MAGÍSTER EN FINANZAS
Vitolo, Giancarlo [Parte I mediante el método de múltiplos], Avila Balucani, Daniel Alejandro [Parte II mediante el método de flujo de caja descontado]
El trabajo desarrollado a continuación, tiene como principal objetivo efectuar la valoración de la empresa AES Panamá SRL y estimar el precio de su acción, a través de la metodología de flujos de caja descontados (FCD). Esta metodología se basa en el modelo multiperiodo de Modigliani-Miller, que consiste principalmente en modelar los flujos de caja libre de la compañía sobre un horizonte de tiempo determinado, para luego ser descontados a su valor presente. Además de estos flujos de caja, el valor presente debe ser determinado para flujos de caja más allá del horizonte de proyección comúnmente llamado valor terminal o perpetuidad. Bajo esta metodología determinar la tasa de descuento es uno de los factores fundamentales. Para esto se deben considerar aspectos claves como el riesgo, comportamientos históricos y el rendimiento mínimo esperado por compradores o vendedores con una expectativa mínima de rentabilidad. En esta valoración utilizamos una tasa de costo de capital, calculada a través de la fórmula WACC, la cual consiste en un promedio ponderado entre el costo de la deuda (kb) y la rentabilidad exigida por los accionistas (kp). Para realizar la valoración a través de esta metodología, es necesario determinar una estructura de capital objetivo a largo plazo para la compañía, la cual puede obtenerse en base al análisis retrospectivo de las cifras presentadas en sus estados financieros. Como parte de la valoración completa de la empresa bajo este método, debemos identificar y adicionar sus activos prescindibles (activos no necesarios para la operación de la empresa) y ajustar el exceso o déficit de capital de trabajo que haya al momento de la valoración. Como último paso en esta valoración, se determina el valor económico del patrimonio restando al valor obtenido de la empresa el valor presente de la deuda financiera al momento de la valoración. Adicionalmente a la valoración a través del método de FCD, estimaremos el valor de la empresa AES Panamá SRL y el precio de su acción por el método de múltiplos. Este método consiste básicamente en estimar el valor de una empresa analizando la relación de las razones financieras de compañías que pertenecen a la misma industria tomadas como benchmark. AES Panamá, es una empresa que genera y vende electricidad en el mercado panameño y mercado eléctrico regional (MER), es la mayor compañía de generación en Panamá en términos de capacidad instalada y energía despachada promedio, donde el 93% de la capacidad firme de las plantas de la Compañía se encuentra contratada bajo varios acuerdos de compra - venta de potencia y energía con compañías de distribución, grandes clientes y la compañía generadora AES Changuinola. La energía excedente es vendida en el mercado ocasional a los precios establecidos en el mismo (precio spot) Para la ejecución de esta valoración se usaron los estados financieros de la empresa y de compañías que componen la industria desde el 30 de junio de 2013 hasta el 30 de septiembre de 2017, por otra parte, se extrajeron los datos presentados por la Bolsa de Valores de Panamá, donde pudimos compilar la información sobre de la estructura de capital, el patrimonio económico, principales competidores de la industria y estructura de la deuda y demás información necesaria para poder analizar, modelar y realizar las proyecciones de los flujos de caja de la empresa con un horizonte de evaluación de 5 años. Ya finalizada la valoración de AES Panamá al 30 de junio de 2017 con diferentes metodologías, obtuvimos como resultados que con el método de flujos de caja descontados el valor de la empresa o del patrimonio es de USD $1,067,808 en miles y un precio por acción de USD $4.97, por otro lado, el método de valoración en base a múltiplos de la industria, el ratio que presenta una mejor estimación es el del precio sobre ventas (Price to sales ratio) arrojando una estimación del valor de la empresa por USD $1,032,656 en miles y un precio por acción de USD $2.98. Debido a que AES Panamá es una empresa que no tranza o cotiza sus acciones en la Bolsa, actualmente no contamos con el valor efectivo de la acción a la fecha, solo tenemos como referencia en valor contable del precio de la acción. A pesar de no tener información pública comparable de la compañía, luego de realizar los procedimientos de valoración y analizar las estimaciones, consideramos que los resultados son lo suficientemente precisos para confiar en los valores que arrojan las diferentes metodologías aplicadas
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35

Ležák, Petr. "Testovací implementace protokolu ACP." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2012. http://www.nusl.cz/ntk/nusl-219478.

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In general this master’s thesis deals with access control methods and their individual modules and in particular with authentication of supplicants. There are listed authentication methods useful in the implementation of the ACP protocol. ACP protocol is also discussed including possibilities and uses. ACP message format is described in detail with AVP format and types. The transaction mechanism is also mentioned here. The main part of the thesis is focused on software design for protocol testing. Possibilities of the testing are discussed and test scenarios are suggested. Consequently, requirements for test software are listed and its implementation is designed. Furthermore, there is technical documentation of the program. The main ideas used in the program are explained in it. The purpose of each part of the program is written including links between them. Finally, there is a manual for the program. It also contains an illustrative example describing how to make and test a simple scenario of the authentication.
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36

Buresta, Pietro. "Sicurezza e crittografia dei sistemi LoRaWAN." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2021.

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La tesi ha lo scopo di fornire le principali generalità sui sistemi LoRaWAN, per poi concentrarsi sui metodi distribuzione delle chiavi, sulla crittografia relativa alle comunicazioni e sui possibili attacchi che una rete LoRa potrebbe subire. Lo scopo principale infatti è quello di approfondire l' aspetto della sicurezza di rete e i metodi utilizzati da tali sistemi per cercare di garantire comunicazioni sicure e affidabili.
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37

Wanner, Alexander [Verfasser]. "Seismic attenuation system (AEI-SAS) for the AEI 10 m prototype / Alexander Wanner." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2013. http://d-nb.info/1036694690/34.

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38

Berggren, Emil, and Tobias Gustafson. "Jämförelse av GPGPU-ramverk och AES-metoder : Jämförelse av GPGPU-ramverk och AES-metoder för att besvara vilka GPGPU-ramverk och vilken AES-metod som bör rekommenderas för AES-kryptering med GPGPU." Thesis, Tekniska Högskolan, Högskolan i Jönköping, JTH, Datateknik och informatik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-35856.

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Sammanfattning Bakgrund - Dagens processorer börjar närma sig gränsen för hur höga klockfrekvenser de kan köras i. Detta har lett till att processorer har fått fler kärnor för att kunna exekvera flera processer parallellt med flertrådade applikationer. Det finns dock ofta en stor mängd oanvänd beräkningskraft under långa perioder då datorn är igång som ligger i grafikprocessorn, GPU. Då en GPU kan köra tusentals många fler trådar på samma gång än en CPU har ramverk för att göra mer generella beräkningar på GPU utvecklats, dessa kallas för GPGPU-ramverk. Då varje kärna på en GPU inte är lika stark som på en CPU ligger vinsten i att använda algoritmer som går bra att parallellisera. En sådan algoritm är krypteringsalgoritmen AES som är en av de säkraste och vanligaste krypteringsalgoritmerna som används idag. Syfte – Med hjälp av GPU-accelerering kan man kryptera med AES snabbare än med en traditionell CPU-lösning. För att göra GPU-accelereringen så effektiv som möjligt undersöker detta examensarbete vilken AES-metod samt vilket GPGPU-ramverk man bör välja. Metod – För att undersöka vilken/vilka AES-metoder samt vilka GPGPU-ramverk som var lämpliga att använda för denna undersökning gjordes två litteraturstudier. Utifrån data som litteraturstudierna gav genomfördes experiment för att jämföra de valda GPGPU-ramverken med den valda AES-metoden som ansågs vara mest lämpliga. Resultat – Från litteraturstudierna kom det fram att OpenCL och CUDA blir de rekommenderade GPGPU-ramverken och att CTR blir den rekommenderade AES-metoden för AES-kryptering med GPGPU-programmering. Utifrån experimenten som genomförts kunde det konstateras att CUDA är ett effektivare GPGPU-ramverk än OpenCL för AES-CTR på det testade grafikkortet, GTX 560. Implikationer – CUDA är snabbare vid större filer för att OpenCL begränsas mer av dataöverföringshastigheten än CUDA på ett GTX 560. Begränsningar – Experimenten genomfördes endast på ett grafikkort från Nvidia. Eftersom Nvidia inte har något intresse i att optimera för andra GPGPU-ramverk så kunde inte testresultaten från OpenCL verifieras med externa verktyg. Detta p.g.a. att Nvidias verktyg inte längre stödjer debugging eller profiling för OpenCL. Nyckelord – Processorer, GPGPU, AES, CTR, OpenCL, CUDA, GPGPU-ramverk
Abstract Background - Processors today are approaching the limit for how high clockfrequences they can run. This has led to that instead of trying to make them run faster they are instead made with multiple cores so they can utilize parallelization by running several threads in parallel. However aside from the CPU there is still the graphics card which has a large amount of unused computing power for long durations of time while the computer is active. While a GPU might not have as quick processors it instead has several thousands of them at the same time than a CPU which have led to the development of GPGPU-frameworks to use that potential parallelization. The profit in this lies in using algorithms and code functions that got high potential parallelization, one of which is the AES encryption algorithm. AES is one of the most widely used encryption algorithms today and also considered to be one of the most secure. Purpose – By using GPGPU-acceleration the encryption speed of AES is higher than by using a traditional CPU approach. To make the GPU-acceleration as effective as possible this study looks into which AES-method and which GPGPU-framework that should be chosen during development. Method – This study makes two literature studies to determine which AES-methods and which GPGPU-frameworks that are viable for GPU-acceleration of AES. Afterwards this study conducts experiments to determine which of these GPGPU-frameworks are the most effective. Findings – The conclusion drawn from the literature study is that the CTR-method among the AES-methods is preferable due to its parallelization potential and high security measures. Among the current GPGPU-frameworks only two frameworks satisfies the criteria determined from the literature study and those are CUDA and OpenCL. From the experiment the conclusion is thereafter drawn that of the two GPGPU-frameworks CUDA is more effective due to the bandwidth limits that OpenCL have compared to CUDA. This conclusion is valid on at least the tested graphics card, GTX 560. Implications – CUDA is faster at larger file sizes than OpenCL due to limited data transfer speed in OpenCL on a GTX 560. Limitations – The experiments were only conducted on one graphics card from Nvidia due to hardware constraints in that CUDA can only be run on Nvidia hardware. Due to this hardware constraint and Nvidia’s lack of support in their tools for debugging and profiling of OpenCL the results from the testing of OpenCL couldn’t be verified using external tools. Keywords – Processor, GPGPU, AES, CTR, OpenCL, CUDA, GPGPU-framework
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39

Pacheco-Rodriguez, Gustavo. "Synthesis, purification and characterization of small mono(ADP-ribosyl)ated molecules in the ADP-ribose elongation reaction catalyzed by poly(ADP-ribose)polymerase." Thesis, North Texas State University, 1993. https://digital.library.unt.edu/ark:/67531/metadc798168/.

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The ADP-ribose elongation catalyzed by poly(ADP-ribose) polymerase (PARP) [EC 2.2.2.30] has been partially characterized utilizing mono (ADP-ribosyl)ated polyamines. Arginine methyl ester (AME)-(ADP-ribose) and agmatine (AGMT)-(ADP-ribose) were synthesized enzymatically with a eukarytic mono(ADP-ribosyl) transferase and cholera toxin, respectively.
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40

Griffiths, Michael Lee. "Multivariate calibration for ICP-AES." Thesis, University of Plymouth, 2001. http://hdl.handle.net/10026.1/1942.

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The analysis of metals is now a major application area for ICP-AES, however, the technique suffers from both spectral and non-spectral interferences. This thesis details the application of univariate and multivariate calibration methods for the prediction of Pt, Pd, and Rh in acid-digested and of Au, Ag and Pd in fusion-digested autocatalyst samples. Of all the univariate calibration methods investigated matrix matching proved the most accurate method with relative root mean square errors (RRMSEs) for Pt, Pd and Rh of 2.4, 3.7, and 2.4 % for a series of synihelic lest solutions, and 12.0, 2.4, and 8.0 % for autocatalyst samples. In comparison, the multivariate calibration method (PLSl) yielded average relative errors for Pt, Pd, and RJi of 5.8, 3.0, and 3.5 % in the test solutions, and 32.0, 7.5, and 75.0 % in the autocatalyst samples. A variable selection procedure has been developed enabling multivariate models to be built using large parts of the atomic emission spectrum. The first stage identified and removed wavelengths whose PLS regression coefficients were equal to zero. The second stage ranked the remaining wavelengths according to their PLS regression coefficient and estimated standard error ratio. The algorithms were applied to the emission spectra for the determination of Pt, Pd and Rh in a synthetic matrix. For independent test samples variable selection gave RRMSEs of 5.3, 2.5 and 1.7 % for Pt, Pd and Rh respectively compared with 8.3, 7.0 and 3.1 % when using integrated atomic emission lines. Variable selection was then applied for the prediction of Au, Ag and Pd in independent test fusion digests. This resulted in RRMSEs of 74.2, 8.8 and 12.2 % for Au, Ag and Pd respectively which were comparable to those obtained using a more traditional univariate calibration approach. A preliminary study has shown that calibration drift can be corrected using Piecewise Direct Standardisation (PDS). The application of PDS to synthetic test samples analysed 10 days apart resulted in RRMSEs of 4.14, 3.03 and 1.88%, compared to 73.04, 44.39 and 28.06 % without correction, for Pt, Pd, and Rh respectively.
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41

Tasini, Andrea. "Crittografia simmetrica: il sistema AES." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amslaurea.unibo.it/2641/.

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42

Chieco, Davide. "L'algoritmo advanced encryption standard (AES)." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amslaurea.unibo.it/7274/.

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43

Cherry, Shirley J. "AEC – Is It All That?" Digital Commons @ East Tennessee State University, 2006. https://dc.etsu.edu/etsu-works/2485.

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44

Pogosian, Anna, and Katya Mehyeddine. "Marknadsanalys av AED i Norden." Thesis, KTH, Skolan för teknik och hälsa (STH), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-190937.

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Varje år drabbas ca 10 000 människor av hjärtstopp utanför sjukhus och av dem är det endast 500 som överlever. Sannolikheten för överlevnad ökar upp mot 75% vid användning av hjärtstartare.   Kandidatexamensarbetet gjordes i uppdrag av Servicia Medical, där målet var att analysera den nordiska marknaden för AED, utifrån att undersöka största återförsäljarna, attraktiva platser för placering av AED, prisutveckling och tillväxt. Marknadsundersökningen omfattar enbart Sverige, Finland och Danmark. För att ta reda på vilka återförsäljare som är störst utfördes en webbaserad informationssökning, samt telefonsamtal och mejlväxling med generalagenter. Data för attraktiva platser och tillväxt erhölls från olika verksamheter. Intervjuer, mejl och telefonsamtal utnyttjades för att få fram prisutvecklingen.   Återförsäljarna delades in i två kategorier, där den ena avsåg företag som har fokus på första hjälpen och den andra företag med ospecifik fokusering.  Information som krävdes för att undersöka vilka platser som är mest attraktiva, samt tillväxten i Finland och Danmark saknades. Gator och torg, tåg, buss, båt och spårtrafik är de mest attraktiva platserna för placering av AED i Sverige. Under de senaste fem åren har priset sjunkit i Sverige och Danmark. Tillväxten i dessa länder är olika där Sverige har en växande trend, medan Danmark har en ojämn och otydlig. Ingen slutsats kan dras för tillväxten i Finland.
Each year about 10 000 people get a cardiac arrest outside hospital and only 500 of them survives. Defibrillators are an efficient treatment for cardiac arrest, where the probability of survival increases up to 75%.   This Bachelor project is done on behalf of Servicia Medical, where the goal is to analyze the Nordic market of AED by investigating the greatest retailers, the most attractive locations to place AED, price development and growth. The market research covers only Sweden, Finland and Denmark. To discover the greatest retailers, web-based research was performed as well as phone-calls and e-mail exchanges with general agents. Data for analyzing attractive locations and growth were obtained from various businesses. Interviews, e-mails and phone calls were used to derive price development.   The found retailers were divided into two categories. The first category was companies that focus on first aid and the second one had a non-specific focus. Some Finnish and Danish businesses had a lack of required information to examine the most attractive locations and growth. Streets and squares, trains, busses, boats and rail traffic are the most attractive locations in Sweden to place AED. During the past five years prices have decreased in Sweden and Denmark. The growth in these countries is different. Whilst Sweden has a growing trend, Denmark has an uneven and unclear trend. No conclusion can be drawn for the growth in Finland.
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45

Osorio, Gonzales Augusta. "ABP lactancia materna - regresión lineal." En Blanco y Negro, 2012. http://repositorio.pucp.edu.pe/index/handle/123456789/117278.

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El objetivo que persigue el ABP “Lactancia materna” es el de poner al alcance de los alumnos de un curso de Estadística general, las herramientas básicas que le permitan establecer y analizar una recta de regresión lineal entre dos variables cuantitativas. El escenario o problema sobre el que se baso el ABP en el curso fue el resaltar la importancia de la Lactancia Materna, tema no vinculado normalmente con la Estadística, pero familiar para los alumnos (especialmente las damas), por lo cual en la ejecución del ABP se incorporó la temática relacionada con la poca información e interés que muestran los varones en el tema. En el escenario, se creó una ONG ficticia llamada ALACTAR, la que estaba interesada en crear un tríptico de información dirigida a los varones, en el que se resalten los beneficios que proporciona la Lactancia Materna en el infante y la madre que da de lactar. Entre los temas a resaltar, se encuentran el incremento del Coeficiente Intelectual, la disminución de la Obesidad Infantil, la disminución de la Diabetes Juvenil y la disminución en el riesgo de la madre de contraer Cáncer de ovarios. La idea es utilizar datos de investigaciones que se han hecho en el país que tiene en su poder ALACTAR y que aún no se han procesado apropiadamente. Dentro de la metodología ABP, se usaron como herramientas las siguientes: foros, horas de trabajo en aula y la confección de un trabajo final que consiste en un tríptico o póster para ALACTAR.
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46

Thorne, N. M. H. "(ADP-ribosyl)ation and Ap4A." Thesis, University of Bath, 1988. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383797.

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47

Miers, Charles Christian. "Modelo simplificado do cifrador AES." Florianópolis, SC, 2002. http://repositorio.ufsc.br/xmlui/handle/123456789/84201.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Ciência da Computação.
Made available in DSpace on 2012-10-20T07:12:45Z (GMT). No. of bitstreams: 1 190173.pdf: 1149637 bytes, checksum: 3dba892952cb5e2fc288d4bd2af2f87f (MD5)
Nesta dissertação é descrito o desenvolvimento de um modelo
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48

Donald, Emily J. "Experiential Theories: AEDP and EFT." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etsu-works/4945.

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49

Di, Paola Simone. "The mammalian ADP-ribosyl proteome and characterisation of novel putative intracellular mono-ADP-ribosyl transferases." Thesis, Open University, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.552798.

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Protein mono-ADP-ribosylation is a reversible post-translational modification that has been implicated in the regulation of different cell functions, including signal transduction, protein trafficking, and immune responses. In mammals, three families of proteins catalyse this reaction: the ecto-monoARTs/ARTCs, some members of the PARP/ARTD family and some members of the sirtuin family. In this study, I have been focused on the characterisation of human P ARP 161 ARTD 15 and hamster cARTC2.1. Moreover, I have set up novel technologies for the study of intracellular ADP- ribosylation reactions. I have shown that PARP16/ARTD15 is a mono-ADP-ribosyl transferase that localises to the endoplasmic reticulum, where it binds karyopherin-B 1, a pivotal nuclear transport receptor. Moreover, I have also shown that PARP16/ARTD15 induces the mono-ADP-ribosylation of karyopherin-B 1, defining the first substrate for this enzyme. These data suggest a novel regulatory mechanism of karyopherin-Bl functions mediated by PARP16/ARTD15 mono-ADP-ribosyl transferase activity. In parallel, I have also characterised the activity and the intracellular localisation of the hamster isoform cARTC2.1. This enzyme has both NAD-glycohydrolase (NADase) and arginine-specific ADP-ribosyltransferase (ART) activities. I have generated a double mutant of cARTC2.1 by substituting both the glutamates of the EKE active-site motif and showed that this mutated protein loses ART activity, while correctly localise to the plasma membrane. Importantly, we have shown that endogenous ADP-ribosyltransferase is indeed expressed and active in a subset of CHO cells. The subset of ART-positive cells, which represented 5% of the total cells, is tightly maintained in the CHO cell population and shows higher level of cARTC2.1 transcript compared to the ART- negative population. Moreover, I have demonstrated that cARTC2.1 catalyses the mono-ADP- ribosylation of GRP78/BiP, a protein with a central role in different cell functions. This finding represents the first demonstration of the ARTC-mediated GRP78/BiP modification. Finally, I have set up experimental conditions for GST-macro domain based approaches. I have shown that the cross-linking of GST-macro domain to the sepharose resin improves ADP- ribosylated substrate isolation and analysis by macro-based pull-down assay. Moreover, I have demonstrated the suitability of GST-macro domain to label endogenous ADP-ribosylated proteins by a far-western blotting approach. These data established that GST -macro domain is a powerful tool in the study of ADP-ribosylation reactions at protein level.
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50

Morrison, Alan R. "Poly(ADP)-Ribose Polymerase Activity in the Eukaryotic Mono-ADP-Ribosyl Transferase, ART2: a Dissertation." eScholarship@UMMS, 2003. https://escholarship.umassmed.edu/gsbs_diss/126.

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The glycophosphatidylinositol(GPI)-linked membrane protein ART2 is an antigenic determinant for T lymphocytes that regulate the expression of diabetes in the BB/W rat model. Though little is understood of the physiologic role of ART2 on T lymphocytes, ART2 is a member of the mono-ADP-ribosyl transferase subgroup ofthe ADP-ribosyl transferase (ART) protein family. The ART protein family, which traditionally has been divided into mono-ADP-ribosyl transferases (mono-ARTs), poly(ADP)-ribose polymerases (PARPs), and ADP-ribosyl cyclases, influences various aspects of cellular physiology including: apoptosis, DNA damage repair, chromatin remodeling, telomere replication, cellular transport, immune regulation, neuronal function, and bacterial virulence. A structural alignment of ART2.2 with chicken PARP indicated the potential for ART2.2 to catalyze ADP-ribose polymers in an activity thought to be specific to the PARP subgroup and important for their regulation of nuclear processes. Kinetic studies determined that the auto-ADP-ribosyl transferase activity of ART2.2 is multitmeric and heterogeneous in nature. Hydroxylamine-cleaved ADP-ribose moieties from the ART2.2 multimers ran as polymers on a modified sequencing gel, and digestion of the polymers with snake-venom phosphodiesterase produced AMP and the poly(ADP)ribose-specific product, PR-AMP, which was resolved by analytical HPLC and structurally confirmed by ESI-MS. The ratio of AMP to PR-AMP was higher than that of PARP raising the possibility that the ART2.2 polymers had a different branching structure than those of PARP. This alternative branching was confirmed by the presence of ribose phosphate polymers in the snake venom phophodiesterase treated samples. The site of the auto-poly(ADP)-ribose modification was determined to be R185, a residue previously proposed to influence the level of auto-ADP ribosylation of ART2.2 by mutational analysis. These data provide the first demonstration of a hybrid between mono-ARTs and PARPs and are the earliest indication that PARP-like enzymes can exist outside the nucleus and on the cell surface.
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