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Journal articles on the topic 'Adsorbed antigen'

Author: Grafiati

Published: 14 March 2023

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1

Zhang, Zhigang, Tianying Zhang, Lu Cao, et al. "Simultaneous in situ visualization and quantitation of dual antigens adsorbed on adjuvants using high content analysis." Nanomedicine 14, no. 19 (2019): 2535–48. http://dx.doi.org/10.2217/nnm-2019-0016.

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Aim: Traditional antigenicity assay requires antigen recovery from the particulate adjuvants prior to analysis. An in situ method was developed for interrogating vaccine antigens with monoclonal antibodies while being adsorbed on adjuvants. Materials & methods: The fluorescence imaging-based high content analysis was used to visualize the antigen distribution on adjuvant agglomerates and to analyze the antigenicity for adsorbed antigens. Results: Simultaneous visualization and quantitation were achieved for dual antigens in a bivalent human papillomavirus vaccine with uniquely labeled anti

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2

Laera, Donatello, Camilla Scarpellini, Simona Tavarini, et al. "Maturation of Aluminium Adsorbed Antigens Contributes to the Creation of Homogeneous Vaccine Formulations." Vaccines 11, no. 1 (2023): 155. http://dx.doi.org/10.3390/vaccines11010155.

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Although aluminium-based vaccines have been used for almost over a century, their mechanism of action remains unclear. It is established that antigen adsorption to the adjuvant facilitates delivery of the antigen to immune cells at the injection site. To further increase our understanding of aluminium-based vaccines, it is important to gain additional insights on the interactions between the aluminium and antigens, including antigen distribution over the adjuvant particles. Immuno-assays can further help in this regard. In this paper, we evaluated how established formulation strategies (i.e.,

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Oksanich, A. S., A. G. Krasko, T. G. Samartseva, E. L. Gasich, and G. M. Ignatyev. "The use of quantitative enzyme-linked immunosorbent assay for the determination of S-antigen concentration in whole-virion inactivated adsorbed coronavirus vaccines." Biological Products. Prevention, Diagnosis, Treatment 22, no. 4 (2022): 405–13. http://dx.doi.org/10.30895/2221-996x-2022-22-4-405-413.

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The severe consequences and high mortality of COVID-19 prompted the development of a wide range of preventive vaccines. The first vaccines to be tested were developed in China and formulated as inactivated SARS-CoV-2 adsorbed on aluminium hydroxide. One of the quality indicators for inactivated adsorbed vaccines is the degree of adsorption, which can be used to control the content not only of non-adsorbed antigen, but also of specific antigen in one dose of a vaccine.The aim of the study was to investigate the possibility of desorbing SARS-CoV-2 antigen from formulated adsorbed vaccines and th

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4

TRUJILLO, Mary, Luz M. SALAZAR, and Jesús VALENCIA. "VACCINE FORMULATION: ADSORPTION OF <I>Plasmodium falciparum</I> MSP-1 PEPTIDE 1585 ON ALUMINIUM HYDROXIDE." Vitae 18, no. 2 (2011): 183–91. http://dx.doi.org/10.17533/udea.vitae.10070.

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The Plasmodium falciparum merozoite surface protein 1 has been studied due to its potential to become a vaccine; likewise, the peptide 1585 which is located in the 42-kDa amino-terminal fragment induces protective immunity in primates. Despite the importance of antigen adsorption in the formulation and production of vaccines containing aluminium adjuvant, the protein fragment adsorption on aluminium hydroxide has not been thoroughly studied. Electrostatic attraction, hydrophobic interaction and ligand exchange have been identified as the major mechanisms involved in antigen retention on the ad

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5

Miletich, JP, and GJ Jr Broze. "Human plasma protein Z antigen: range in normal subjects and effect of warfarin therapy." Blood 69, no. 6 (1987): 1580–86. http://dx.doi.org/10.1182/blood.v69.6.1580.bloodjournal6961580.

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In contrast to the other well-studied vitamin K-dependent proteins that circulate in plasma, protein Z antigen is much more variable. The concentration in plasmas collected in EDTA from 455 normal, healthy donors is normally distributed with a mean of 2.9 micrograms/mL (46 nmol/L) and a SD of 1.0 microgram/mL (95% interval of 32% to 168% of the mean). No significant correlation to age or sex could be detected. In comparison, the concentration of protein C antigen measured with the same type of assay on the same 455 samples has a log normal distribution with a mean of 4.0 micrograms/mL (65 nmol

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Dunstan, RA, MB Simpson, RW Knowles, and WF Rosse. "The origin of ABH antigens on human platelets." Blood 65, no. 3 (1985): 615–19. http://dx.doi.org/10.1182/blood.v65.3.615.615.

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Abstract ABH antigens are present on platelets from individuals of the corresponding red cell phenotype, but the extent to which these antigens are intrinsic or adsorbed remains undefined. To evaluate platelets for intrinsic H substance, an IgM mouse monoclonal antibody against type 2H chain (the intrinsic H structure found on erythrocytes) was labeled with 125I and incubated with platelets from donors of different ABO type. The antibody showed dose-response saturation curves, and binding to platelets paralleled that of the red cell ABO type, with O greater than B greater than A1 greater than

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7

Dunstan, RA, MB Simpson, RW Knowles, and WF Rosse. "The origin of ABH antigens on human platelets." Blood 65, no. 3 (1985): 615–19. http://dx.doi.org/10.1182/blood.v65.3.615.bloodjournal653615.

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ABH antigens are present on platelets from individuals of the corresponding red cell phenotype, but the extent to which these antigens are intrinsic or adsorbed remains undefined. To evaluate platelets for intrinsic H substance, an IgM mouse monoclonal antibody against type 2H chain (the intrinsic H structure found on erythrocytes) was labeled with 125I and incubated with platelets from donors of different ABO type. The antibody showed dose-response saturation curves, and binding to platelets paralleled that of the red cell ABO type, with O greater than B greater than A1 greater than A1B great

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8

Werthén, Maria, and Håkan Nygren. "Cooperativity in the antibody binding to surface-adsorbed antigen." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1162, no. 3 (1993): 326–32. http://dx.doi.org/10.1016/0167-4838(93)90298-6.

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9

Miletich, JP, and GJ Jr Broze. "Human plasma protein Z antigen: range in normal subjects and effect of warfarin therapy." Blood 69, no. 6 (1987): 1580–86. http://dx.doi.org/10.1182/blood.v69.6.1580.1580.

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Abstract In contrast to the other well-studied vitamin K-dependent proteins that circulate in plasma, protein Z antigen is much more variable. The concentration in plasmas collected in EDTA from 455 normal, healthy donors is normally distributed with a mean of 2.9 micrograms/mL (46 nmol/L) and a SD of 1.0 microgram/mL (95% interval of 32% to 168% of the mean). No significant correlation to age or sex could be detected. In comparison, the concentration of protein C antigen measured with the same type of assay on the same 455 samples has a log normal distribution with a mean of 4.0 micrograms/mL

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10

Zhao, Xiubo, Fang Pan, Luis Garcia-Gancedo, et al. "Interfacial recognition of human prostate-specific antigen by immobilized monoclonal antibody: effects of solution conditions and surface chemistry." Journal of The Royal Society Interface 9, no. 75 (2012): 2457–67. http://dx.doi.org/10.1098/rsif.2012.0148.

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The specific recognition between monoclonal antibody (anti-human prostate-specific antigen, anti-hPSA) and its antigen (human prostate-specific antigen, hPSA) has promising applications in prostate cancer diagnostics and other biosensor applications. However, because of steric constraints associated with interfacial packing and molecular orientations, the binding efficiency is often very low. In this study, spectroscopic ellipsometry and neutron reflection have been used to investigate how solution pH, salt concentration and surface chemistry affect antibody adsorption and subsequent antigen b

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11

Bellanti, Joseph A., Feng-Ying C. Lin, Chiayung Chu, et al. "Phase 1 Study of a Recombinant Mutant Protective Antigen of Bacillus anthracis." Clinical and Vaccine Immunology 19, no. 2 (2011): 140–45. http://dx.doi.org/10.1128/cvi.05556-11.

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ABSTRACTA phase 1 study of a recombinant mutant protective antigen (rPA) vaccine was conducted in 186 healthy adults aged 18 to 45 years. Volunteers were randomized to receive one of three formulations of rPA (formalin treated, alum adsorbed, or both), in 10- or 20-μg dosages each, or the licensed vaccine, AVA. Three injections were given at 2-month intervals and a 4th 1 year after the 3rd. Vaccinees were examined at the clinic once following each injection, at 48 to 72 h postinjection. Adverse reactions were recorded in diaries for 7 days. Sera were collected before each injection and 1 week

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12

Xie, Hang, Ihsan Gursel, Bruce E. Ivins, et al. "CpG Oligodeoxynucleotides Adsorbed onto Polylactide-Co-Glycolide Microparticles Improve the Immunogenicity and Protective Activity of the Licensed Anthrax Vaccine." Infection and Immunity 73, no. 2 (2005): 828–33. http://dx.doi.org/10.1128/iai.73.2.828-833.2005.

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ABSTRACT To reduce the biothreat posed by anthrax, efforts are under way to improve the protection afforded by vaccination. This work examines the ability of immunostimulatory CpG oligodeoxynucleotides (ODN) adsorbed onto cationic polylactide-co-glycolide (PLG) microparticles (CpG ODN-PLG) to accelerate and boost the protective immunity elicited by Anthrax Vaccine Adsorbed (AVA, the licensed human anthrax vaccine). The results indicate that coadministering CpG ODN-PLG with AVA induces a stronger and faster immunoglobulin G response against the protective antigen of anthrax than AVA alone. Immu

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Morefield, Garry L., Harm HogenEsch, J. Paul Robinson, and Stanley L. Hem. "Distribution of adsorbed antigen in mono-valent and combination vaccines." Vaccine 22, no. 15-16 (2004): 1973–84. http://dx.doi.org/10.1016/j.vaccine.2003.10.040.

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14

Beaman, Blaine L., and LoVelle Beaman. "Filament Tip-Associated Antigens Involved in Adherence to and Invasion of Murine Pulmonary Epithelial Cells In Vivo and HeLa Cells In Vitro by Nocardia asteroides." Infection and Immunity 66, no. 10 (1998): 4676–89. http://dx.doi.org/10.1128/iai.66.10.4676-4689.1998.

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ABSTRACT The interactions of Nocardia asteroides GUH-2 with pulmonary epithelial cells of C57BL/6 mice and with HeLa cells were studied. Electron microscopy demonstrated that only the tips of log-phase cells penetrated pulmonary epithelial cells following intranasal administration, and nocardiae were recovered from the brain. Coccobacillary cells neither invaded nor disseminated. Serum from immunized mice (IMS) decreased attachment to and penetration of pulmonary epithelial cell surfaces by log-phase GUH-2 and inhibited spread to the brain. IMS was adsorbed against stationary-phase cells. West

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15

Ukidve, Anvay, Zongmin Zhao, Alexandra Fehnel, et al. "Erythrocyte-driven immunization via biomimicry of their natural antigen-presenting function." Proceedings of the National Academy of Sciences 117, no. 30 (2020): 17727–36. http://dx.doi.org/10.1073/pnas.2002880117.

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Erythrocytes naturally capture certain bacterial pathogens in circulation, kill them through oxidative stress, and present them to the antigen-presenting cells (APCs) in the spleen. By leveraging this innate immune function of erythrocytes, we developed erythrocyte-driven immune targeting (EDIT), which presents nanoparticles from the surface of erythrocytes to the APCs in the spleen. Antigenic nanoparticles were adsorbed on the erythrocyte surface. By engineering the number density of adsorbed nanoparticles, (i.e., the number of nanoparticles loaded per erythrocyte), they were predominantly de

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16

Wu, J. Y., B. H. Gardner, C. I. Murphy, et al. "Saponin adjuvant enhancement of antigen-specific immune responses to an experimental HIV-1 vaccine." Journal of Immunology 148, no. 5 (1992): 1519–25. http://dx.doi.org/10.4049/jimmunol.148.5.1519.

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Abstract The adjuvant activity of a single highly purified saponin from the soap bark tree Quillaja saponaria was evaluated by using it as a component in an experimental vaccine containing rHIV-1 envelope protein (HIV-1 160D) adsorbed to alum. BALB/c mice immunized with experimental vaccine formulations containing the saponin adjuvant QS-21 produced significantly higher titers of antibodies than mice vaccinated with only the alum-adsorbed HIV-1 160D. Potent amnestic antibody responses to HIV-1 viral proteins were also induced. Ag-specific proliferative responses to recombinant proteins and to

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17

Kao, KJ, DJ Cook, and JC Scornik. "Quantitative analysis of platelet surface HLA by W6/32 anti-HLA monoclonal antibody." Blood 68, no. 3 (1986): 627–32. http://dx.doi.org/10.1182/blood.v68.3.627.627.

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Abstract Class I molecules of human major histocompatibility complex (HLA) are the most important antigenic system in determining the survival of transfused platelets in alloimmunized patients. Platelets with reduced expression of a specific type of HLA antigen may escape specific anti- HLA antibody-mediated destruction. By using 125I-labeled Fab fragments of W6/32 anti-HLA monoclonal antibody and competitive protein binding assays, we measured the range of total HLA concentrations on platelets. In 12 individuals examined, the mean number of HLA-A, B, and C molecules per platelet was 81,587 +/

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18

Kao, KJ, DJ Cook, and JC Scornik. "Quantitative analysis of platelet surface HLA by W6/32 anti-HLA monoclonal antibody." Blood 68, no. 3 (1986): 627–32. http://dx.doi.org/10.1182/blood.v68.3.627.bloodjournal683627.

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Class I molecules of human major histocompatibility complex (HLA) are the most important antigenic system in determining the survival of transfused platelets in alloimmunized patients. Platelets with reduced expression of a specific type of HLA antigen may escape specific anti- HLA antibody-mediated destruction. By using 125I-labeled Fab fragments of W6/32 anti-HLA monoclonal antibody and competitive protein binding assays, we measured the range of total HLA concentrations on platelets. In 12 individuals examined, the mean number of HLA-A, B, and C molecules per platelet was 81,587 +/- 20,016

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19

Frampton, James E., and Susan J. Keam. "Reduced-Antigen, Combined Diphtheria-Tetanus-Acellular Pertussis Vaccine, Adsorbed (Boostrix?? US Formulation)." Pediatric Drugs 8, no. 3 (2006): 189–95. http://dx.doi.org/10.2165/00148581-200608030-00005.

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20

Halperin, Scott A. "Reduced-Antigen, Combined Diphtheria-Tetanus-Acellular Pertussis Vaccine, Adsorbed (Boostrix?? US Formulation)." Pediatric Drugs 8, no. 3 (2006): 196. http://dx.doi.org/10.2165/00148581-200608030-00006.

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21

Theeten, Heidi, Pierre Van Damme, and Marie Van der Wielen. "Reduced-Antigen, Combined Diphtheria-Tetanus-Acellular Pertussis Vaccine, Adsorbed (Boostrix?? US Formulation)." Pediatric Drugs 8, no. 3 (2006): 196. http://dx.doi.org/10.2165/00148581-200608030-00007.

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22

McCormack, Paul L. "Reduced-Antigen, Combined Diphtheria, Tetanus and Acellular Pertussis Vaccine, Adsorbed (Boostrix®)." Drugs 72, no. 13 (2012): 1765–91. http://dx.doi.org/10.2165/11209630-000000000-00000.

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23

Kanzaki, A., H. Kasuya, K. Yamamura, et al. "Antitumor efficacy of oncolytic herpes simplex virus adsorbed onto antigen-specific lymphocytes." Cancer Gene Therapy 19, no. 4 (2012): 292–98. http://dx.doi.org/10.1038/cgt.2011.91.

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24

Scott, Lesley J., and Paul L. McCormack. "Reduced-Antigen, Combined Diphtheria, Tetanus, and Acellular Pertussis Vaccine, Adsorbed (Boostrix®)." BioDrugs 27, no. 1 (2012): 75–81. http://dx.doi.org/10.1007/s40259-012-0009-y.

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25

Owens, T., and B. Fazekas de St Groth. "Participation of L3T4 in T cell activation in the absence of class II major histocompatibility complex antigens. Inhibition by anti-L3T4 antibodies is a function both of epitope density and mode of presentation of anti-receptor antibody." Journal of Immunology 138, no. 8 (1987): 2402–9. http://dx.doi.org/10.4049/jimmunol.138.8.2402.

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Abstract The recognition of many class II major histocompatibility complex (MHC)-associated antigens by T cells requires the participation of the L3T4 molecule. It has been proposed that this molecule acts to stabilize low affinity binding to antigen in association with MHC and thereby increases the avidity of T cell/antigen interactions. By using antibodies against the T cell antigen receptor (TCR) to activate T cells, thereby circumventing the requirement for antigen presenting cells and MHC-associated antigen, we have been able to study the function of L3T4 in the absence of class II MHC. W

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Weissmueller, Nikolas T., Heiko A. Schiffter, Robert C. Carlisle, Christine S. Rollier, and Andrew J. Pollard. "Needle-Free Dermal Delivery of a Diphtheria Toxin CRM197Mutant on Potassium-Doped Hydroxyapatite Microparticles." Clinical and Vaccine Immunology 22, no. 5 (2015): 586–92. http://dx.doi.org/10.1128/cvi.00121-15.

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ABSTRACTInjections with a hypodermic needle and syringe (HNS) are the current standard of care globally, but the use of needles is not without limitation. While a plethora of needle-free injection devices exist, vaccine reformulation is costly and presents a barrier to their widespread clinical application. To provide a simple, needle-free, and broad-spectrum protein antigen delivery platform, we developed novel potassium-doped hydroxyapatite (K-Hap) microparticles with improved protein loading capabilities that can provide sustained local antigen presentation and release. K-Hap showed increas

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27

Qin, Lin, Jun Cao, Kun Shao, et al. "A tumor-to-lymph procedure navigated versatile gel system for combinatorial therapy against tumor recurrence and metastasis." Science Advances 6, no. 36 (2020): eabb3116. http://dx.doi.org/10.1126/sciadv.abb3116.

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Application of cancer vaccines is limited due to their systemic immunotoxicity and inability to satisfy all the steps, including loading of tumor antigens, draining of antigens to lymph nodes (LNs), internalization of antigens by dendritic cells (DCs), DC maturation, and cross-presentation of antigens for T cell activation. Here, we present a combinatorial therapy, based on a α-cyclodextrin (CD)–based gel system, DOX/ICG/CpG-P-ss-M/CD, fabricated by encapsulating doxorubicin (DOX) and the photothermal agent indocyanine green (ICG). Upon irradiation, the gel system exhibited heat-responsive rel

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Rossi, Omar, Maria Grazia Aruta, Alessandra Acquaviva, Francesca Mancini, Francesca Micoli, and Francesca Necchi. "Characterization of Competitive ELISA and Formulated Alhydrogel Competitive ELISA (FAcE) for Direct Quantification of Active Ingredients in GMMA-Based Vaccines." Methods and Protocols 3, no. 3 (2020): 62. http://dx.doi.org/10.3390/mps3030062.

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Generalized modules for membrane antigens (GMMA) represent a technology particularly attractive for designing affordable vaccines against Gram-negative bacteria. We explored such technology for the development of O-antigen-based vaccines against Shigella and nontyphoidal Salmonella. Adsorption of GMMA on Alhydrogel was required for abrogation of pyrogenicity in rabbits, and Shigella sonnei GMMA on Alhydrogel was well tolerated and immunogenic in humans. Quantification of key antigens in formulated vaccines was fundamental for release and to check stability overtime. Traditionally, the direct q

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Matyas, Gary R., Arthur M. Friedlander, Gregory M. Glenn, Stephen Little, Jianmei Yu, and Carl R. Alving. "Needle-Free Skin Patch Vaccination Method for Anthrax." Infection and Immunity 72, no. 2 (2004): 1181–83. http://dx.doi.org/10.1128/iai.72.2.1181-1183.2004.

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ABSTRACT Three immunizations of mice with recombinant protective antigen (rPA) by transcutaneous immunization (TCI) induced long-term neutralizing antibody titers that were superior to those obtained with aluminum-adsorbed rPA. In addition, rPA alone exhibited adjuvant activity for TCI. Forty-six weeks after completion of TCI, 100% protection was observed against lethal anthrax challenge.

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Mollicone, R., T. Caillard, J. Le Pendu, et al. "Expression of ABH and X (Lex) antigens on platelets and lymphocytes." Blood 71, no. 4 (1988): 1113–19. http://dx.doi.org/10.1182/blood.v71.4.1113.1113.

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Abstract We used a panel of reagents, polyclonal and monoclonal antibodies, and lectins to define the expression of the ABH- and Lewis-related specificities on platelets and lymphocytes. We also determined the expression of the alpha 2- and alpha 3-L-fucosyltransferases necessary for their biosynthesis. The antigens that could be detected by immunofluorescence and Western blot analysis were based on type 2 monofucosylated structures. Antibodies directed toward types 1, 3, and 4 ABH-, X- and Lewis-related antigenic determinants were always negative because the small amounts of ABH and Lewis ant

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Mollicone, R., T. Caillard, J. Le Pendu, et al. "Expression of ABH and X (Lex) antigens on platelets and lymphocytes." Blood 71, no. 4 (1988): 1113–19. http://dx.doi.org/10.1182/blood.v71.4.1113.bloodjournal7141113.

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We used a panel of reagents, polyclonal and monoclonal antibodies, and lectins to define the expression of the ABH- and Lewis-related specificities on platelets and lymphocytes. We also determined the expression of the alpha 2- and alpha 3-L-fucosyltransferases necessary for their biosynthesis. The antigens that could be detected by immunofluorescence and Western blot analysis were based on type 2 monofucosylated structures. Antibodies directed toward types 1, 3, and 4 ABH-, X- and Lewis-related antigenic determinants were always negative because the small amounts of ABH and Lewis antigens ads

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32

Karpova, Olga, Nikolai Nikitin, Sergey Chirkov, et al. "Immunogenic compositions assembled from tobacco mosaic virus-generated spherical particle platforms and foreign antigens." Journal of General Virology 93, no. 2 (2012): 400–407. http://dx.doi.org/10.1099/vir.0.036293-0.

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We reported recently that RNA-free spherical particles (SPs) generated by thermal remodelling of tobacco mosaic virus (TMV) are capable of binding GFP to their surface. Here, we show that SPs represent a universal particle platform that can form compositions by binding a diversity of various foreign proteins/epitopes of viral and non-viral origin to their surface. Numerous molecules of a foreign protein linked to the SP surface were revealed by immunogold electron microscopy. Several SP-based compositions were obtained containing one of the following foreign antigens: antigenic determinant A o

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Li, Dongdong, Mengjie Xu, Gaotian Li, et al. "Mg/Al-LDH as a nano-adjuvant for pertussis vaccine: a evaluation compared with aluminum hydroxide adjuvant." Nanotechnology 33, no. 23 (2022): 235102. http://dx.doi.org/10.1088/1361-6528/ac56f3.

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Abstract Background. Layered double hydroxide (LDH) has been demonstrated as a highly efficient antigen platform to induce effective and durable immune response. However, whether LDH nanoparticles could act as an adjuvant for pertussis vaccines is still unknown. Here we evaluated the potential of Mg/Al-LDH as a nano-adjuvant to improve immune response against pertussis and compared it with commercial aluminum hydroxide (AH) adjuvant. Method. The Mg/Al-LDH nanoparticles were synthesized by a hydrothermal reaction. The morphology, structure and size of Mg/Al-LDH were characterized by transmissio

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Alexander, R. J. "Immunological Evidence for Prothrombin in Human Platelets." Thrombosis and Haemostasis 55, no. 02 (1986): 268–70. http://dx.doi.org/10.1055/s-0038-1661534.

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SummaryAn attempt was made to isolate from plasma the platelet surface substrate for thrombin, glycoprotein V (GPV), because a GPV antigen was reported to be present in plasma (3). Plasma fractionation based on procedures for purification of GPV from platelets revealed a thrombin-sensitive protein with appropriate electrophoretic mobility. The protein was purified; an antiserum against it i) reacted with detergent-solubilized platelet proteins or secreted proteins in a double diffusion assay, ii) adsorbed a protein from the supernatant solution of activated platelets, and iii) inhibited thromb

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Tsuruta, Lilian Rumi, Ana Maria Moro, Denise V. Tambourgi, and Osvaldo Augusto Sant’Anna. "Oral Tolerance Induction by Bothrops jararaca Venom in a Murine Model and Cross-Reactivity with Toxins of Other Snake Venoms." Toxins 13, no. 12 (2021): 865. http://dx.doi.org/10.3390/toxins13120865.

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Oral tolerance is defined as a specific suppression of cellular and humoral immune responses to a particular antigen through prior oral administration of an antigen. It has unique immunological importance since it is a natural and continuous event driven by external antigens. It is characterized by low levels of IgG in the serum of animals after immunization with the antigen. There is no report of induction of oral tolerance to Bothrops jararaca venom. Here, we induced oral tolerance to B. jararaca venom in BALB/c mice and evaluated the specific tolerance and cross-reactivity with the toxins o

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Freitas, Natália Erdens Maron, Emily Ferreira Santos, Leonardo Maia Leony, et al. "Double-antigen sandwich ELISA based on chimeric antigens for detection of antibodies to Trypanosoma cruzi in human sera." PLOS Neglected Tropical Diseases 16, no. 3 (2022): e0010290. http://dx.doi.org/10.1371/journal.pntd.0010290.

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Background Enzyme-linked immunosorbent assays (ELISA) are generally the chosen test for Chagas disease (CD) diagnosis; however, its performance depends on the antigen preparation adsorbed to the solid phase, which may lead to false-positive results and cross-reactions. The use of chimeric recombinant antigens can overcome this limitation. Four chimeric antigens from Trypanosoma cruzi (IBMP-8.1, IBMP-8.2, IBMP-8.3 and IBMP-8.4) were developed and evaluated in phase I, II and III studies using indirect ELISA as diagnostic platform. However, peroxidase-labeled secondary anti-human IgG antibody, w

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Dierks, S. E., J. E. Butler, and H. B. Richerson. "Altered recognition of surface-adsorbed compared to antigen-bound antibodies in the ELISA." Molecular Immunology 23, no. 4 (1986): 403–11. http://dx.doi.org/10.1016/0161-5890(86)90138-0.

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Pérez-Betancourt, Yunys, Péricles Marques Araujo, Bianca de Carvalho Lins Fernandes Távora, Daniele Rodrigues Pereira, Eliana Lima Faquim-Mauro, and Ana Maria Carmona-Ribeiro. "Cationic and Biocompatible Polymer/Lipid Nanoparticles as Immunoadjuvants." Pharmaceutics 13, no. 11 (2021): 1859. http://dx.doi.org/10.3390/pharmaceutics13111859.

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Nanostructures have been of paramount importance for developing immunoadjuvants. They must be cationic and non-cytotoxic, easily assembling with usually oppositely charged antigens such as proteins, haptens or nucleic acids for use in vaccines. We obtained optimal hybrid nanoparticles (NPs) from the biocompatible polymer poly(methyl methacrylate) (PMMA) and the cationic lipid dioctadecyl dimethyl ammonium bromide (DODAB) by emulsion polymerization of methyl methacrylate (MMA) in the presence of DODAB. NPs adsorbed ovalbumin (OVA) as a model antigen and we determined their adjuvant properties.

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Hernandez-Franco, Juan F., Imran M. Jan, Malaycia Goldsmith, and Harm HogenEsch. "Optimizing vaccine performance through improved cross-presentation with a nanoparticle adjuvant." Journal of Immunology 208, no. 1_Supplement (2022): 123.03. http://dx.doi.org/10.4049/jimmunol.208.supp.123.03.

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Abstract Activation of CD8 T cells requires presentation of antigenic peptides by MHC I molecules and costimulatory signals provided by CD80 and CD86 on dendritic cells (DCs). Subunit and purified antigen vaccines often fail to induce CD8 T cell responses because the antigens have limited access to the endogenous MHC I pathway, a phenomenon known as antigen cross-presentation. Vaccine adjuvants are being investigated to promote cross-presentation, but adjuvants in currently licensed vaccines are not effective. The goal of this study was to determine if a combination adjuvant (NanoS100) compris

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Wui, Seo Ri, Ara Ko, Ji In Ryu, et al. "The Effect of a TLR4 Agonist/Cationic Liposome Adjuvant on Varicella-Zoster Virus Glycoprotein E Vaccine Efficacy: Antigen Presentation, Uptake, and Delivery to Lymph Nodes." Pharmaceutics 13, no. 3 (2021): 390. http://dx.doi.org/10.3390/pharmaceutics13030390.

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Adjuvant CIA09, composed of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP)-based cationic liposomes and the toll-like receptor 4 agonist de-O-acylated lipooligosaccharide (dLOS), has been shown to enhance antibody and cellular immune responses to varicella-zoster virus (VZV) glycoprotein E (gE), recombinant tuberculosis vaccine antigen, and inactivated Japanese encephalitis vaccine. In this study, we investigated its modes of action using VZV gE as a model antigen. Liposomes adsorbed gE and cooperatively with dLOS promoted endocytosis-mediated cellular uptake of gE by mouse dendritic cells i

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Ovsyannikova, Inna G., V. Shane Pankratz, Robert A. Vierkant, et al. "Human Leukocyte Antigens and Cellular Immune Responses to Anthrax Vaccine Adsorbed." Infection and Immunity 81, no. 7 (2013): 2584–91. http://dx.doi.org/10.1128/iai.00269-13.

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ABSTRACTInterindividual variations in vaccine-induced immune responses are in part due to host genetic polymorphisms in the human leukocyte antigen (HLA) and other gene families. This study examined associations between HLA genotypes, haplotypes, and homozygosity and protective antigen (PA)-specific cellular immune responses in healthy subjects following immunization with Anthrax Vaccine Adsorbed (AVA). While limited associations were observed between individual HLA alleles or haplotypes and variable lymphocyte proliferative (LP) responses to AVA, analyses of homozygosity supported the hypothe

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Skare, Jonathan T., Denise M. Foley, Santiago R. Hernandez, et al. "Cloning and Molecular Characterization of Plasmid-Encoded Antigens of Borrelia burgdorferi." Infection and Immunity 67, no. 9 (1999): 4407–17. http://dx.doi.org/10.1128/iai.67.9.4407-4417.1999.

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ABSTRACT Thirteen independent clones that encode Borrelia burgdorferi antigens utilizing antiserum from infection-immune rabbits were identified. The serum was adsorbed against noninfectiousB. burgdorferi B31 to enrich for antibodies directed against either infection-associated antigens of B. burgdorferi B31 or proteins preferentially expressed during mammalian infection. The adsorption efficiency of the immune rabbit serum (IRS) was assessed by Western immunoblot analysis with protein lysates derived from infectious and noninfectious B. burgdorferi B31. The adsorbed IRS was used to screen a B

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Williamson, E. D., I. Hodgson, N. J. Walker, et al. "Immunogenicity of Recombinant Protective Antigen and Efficacy against Aerosol Challenge with Anthrax." Infection and Immunity 73, no. 9 (2005): 5978–87. http://dx.doi.org/10.1128/iai.73.9.5978-5987.2005.

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ABSTRACT Immunization with a recombinant form of the protective antigen (rPA) from Bacillus anthracis has been carried out with rhesus macaques. Rhesus macaques immunized with 25 μg or more of B. subtilis-expressed rPA bound to alhydrogel had a significantly increased immunoglobulin G (IgG) response to rPA compared with macaques receiving the existing licensed vaccine from the United Kingdom (anthrax vaccine precipitated [AVP]), although the isotype profile was unchanged, with bias towards the IgG1 and IgG2 subclasses. Immune macaque sera from all immunized groups contained toxin-neutralizing

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Wagner, Leslie, Anita Verma, Bruce D. Meade, et al. "Structural and Immunological Analysis of Anthrax Recombinant Protective Antigen Adsorbed to Aluminum Hydroxide Adjuvant." Clinical and Vaccine Immunology 19, no. 9 (2012): 1465–73. http://dx.doi.org/10.1128/cvi.00174-12.

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ABSTRACTNew anthrax vaccines currently under development are based on recombinant protective antigen (rPA) and formulated with aluminum adjuvant. Because long-term stability is a desired characteristic of these vaccines, an understanding of the effects of adsorption to aluminum adjuvants on the structure of rPA is important. Using both biophysical and immunological techniques, we compared the structure and immunogenicity of freshly prepared rPA-Alhydrogel formulations to that of formulations stored for 3 weeks at either room temperature or 37°C in order to assess the changes in rPA structure t

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Kimura, E., Makoto Itoh, Xu-Guang Qiu, Nipul K. Gunawardena, and Mirani V. Weerasooriya. "Filter paper-adsorbed whole blood for Og4C3 antigen capture elisa and its field application." Parasitology International 47 (August 1998): 45. http://dx.doi.org/10.1016/s1383-5769(98)80074-3.

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Amini, Yousef, Bagher Moradi, Mohsen Tafaghodi, Zahra Meshkat, Kiarash Ghazvini, and Mahdi Fasihi-Ramandi. "TB trifusion antigen adsorbed on calcium phosphate nanoparticles stimulates strong cellular immunity in mice." Biotechnology and Bioprocess Engineering 21, no. 5 (2016): 653–58. http://dx.doi.org/10.1007/s12257-016-0326-y.

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Li, Bei, Yafang Tan, Jingyu Guo, et al. "Use of protein microarray to identify gene expression changes ofYersinia pestisat different temperatures." Canadian Journal of Microbiology 57, no. 4 (2011): 287–94. http://dx.doi.org/10.1139/w11-007.

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Yersinia pestis is a bacterium that is transmitted between fleas, which have a body temperature of 26 °C, and mammalian hosts, which have a body temperature of 37 °C. To adapt to the temperature shift, phenotype variations, including virulence, occur. In this study, an antigen microarray including 218 proteins of Y. pestis was used to evaluate antibody responses in a pooled plague serum that was unadsorbed, adsorbed by Y. pestis cultivated at 26 °C, or adsorbed by Y. pestis cultivated at 26 and 37 °C to identify protein expression changes during the temperature shift. We identified 12 proteins

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Mi, Fwu-Long, Shin-Shing Shyu, Chin-Ta Chen, and Jen-Yu Schoung. "Porous chitosan microsphere for controlling the antigen release of Newcastle disease vaccine: preparation of antigen-adsorbed microsphere and in vitro release." Biomaterials 20, no. 17 (1999): 1603–12. http://dx.doi.org/10.1016/s0142-9612(99)00064-2.

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Skoble, Justin, John W. Beaber, Yi Gao, et al. "Killed but Metabolically Active Bacillus anthracis Vaccines Induce Broad and Protective Immunity against Anthrax." Infection and Immunity 77, no. 4 (2009): 1649–63. http://dx.doi.org/10.1128/iai.00530-08.

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ABSTRACTBacillus anthracisis the causative agent of anthrax. We have developed a novel whole-bacterial-cell anthrax vaccine utilizingB. anthracisthat is killed but metabolically active (KBMA). Vaccine strains that are asporogenic and nucleotide excision repair deficient were engineered by deleting thespoIIEanduvrABgenes, renderingB. anthracisextremely sensitive to photochemical inactivation with S-59 psoralen and UV light. We also introduced point mutations into thelefandcyagenes, which allowed inactive but immunogenic toxins to be produced. Photochemically inactivated vaccine strains maintain

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Vitral, Claudia Lamarca, Ana Maria Coimbra Gaspar, and Clara Fumiko Tachibana Yoshida. "Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen." Revista da Sociedade Brasileira de Medicina Tropical 24, no. 2 (1991): 79–85. http://dx.doi.org/10.1590/s0037-86821991000200003.

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Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.

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