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Journal articles on the topic 'Adipose-derived mesenchymal stem cell'

Author: Grafiati

Published: 11 March 2023

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1

Zahran F, Zahran F., El-Deen IM El-Deen IM, Hamed S. Hamed S, and EL-Shenawy A. EL-Shenawy A. "Characterization of Adipogenic Differentiation of Mesenchymal Stem Cell Derived from Mice Adipose Tissue." Indian Journal of Applied Research 3, no. 7 (October 1, 2011): 18–22. http://dx.doi.org/10.15373/2249555x/july2013/7.

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Kawata, Yumiko, Eiji Ikami, Junya Nojima, Shoichiro Kokabu, Tetsuya Yoda, and Tsuyoshi Sato. "Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Irradiated Bone Marrow-Derived Mesenchymal Stem Cells." Journal of Bone Biology and Osteoporosis 4, no. 1 (November 15, 2018): 94–98. http://dx.doi.org/10.18314/jbo.v4i1.1230.

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Adipose-derived Mesenchymal stem cells have emerged as an attractive alternative source of cell therapy. While radiation therapy is an important application for head and neck cancer, the effect of adipose-derived mesenchymal stem cells on irradiated bone marrow-derived Mesenchymal stem cells is still unclear. Herein, we explored how clinical total radiation dose affect gene expression related with differentiation on murine bone marrow-derived mesenchymal stem cells and how murine adipose-derived mesenchymal stem cells affect irradiated murine bone marrow-derived mesenchymal stem cells. The clinical total radiation dose upregulates osterix mRNA expression. Moreover, adiposederived mesenchymal stem cells dramatically promoted the upregulation of osterix mRNA expression whereas inhibited NFATc1 mRNA expression. Taken as a whole, irradiated bone marrow-derived mesenchymal stem cells co-cultured with adipose-derived mesenchymal stem cells may exhibit osteogenic property.

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An, JH, KB Kim, SC Kwon, HJ Kim, MO Ryu, YI Oh, JO Ahn, and HY Youn. "Canine adipose tissue-derived mesenchymal stem cell therapy in a dog with renal Fanconi syndrome." Veterinární Medicína 67, No. 4 (February 16, 2022): 206–11. http://dx.doi.org/10.17221/213/2020-vetmed.

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Renal Fanconi syndrome (RFS) affects the proximal tubular resorption in the nephrons. This causes excessive loss of key solutes through the urine. In a canine patient, we successfully managed the renal tubular acidosis and proteinuria caused by RFS via transplantation of canine adipose tissue-derived mesenchymal stem cells (cAT-MSCs). cAT-MSCs were administered ten times at intervals of 2–4 weeks. The post-therapy check-up revealed that the cAT-MSC treatment improved the renal tubular acidosis and proteinuria. Hence, a cAT-MSC transplant may be considered as an adjuvant therapy in veterinary medicine to initiate and maintain relief of RFS-induced acidosis and proteinuria.

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Gerth, David J., and Seth R. Thaller. "Adipose-Derived Mesenchymal Stem Cells." Journal of Craniofacial Surgery 30, no. 3 (May 2019): 636–38. http://dx.doi.org/10.1097/scs.0000000000005336.

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5

Bunnell, Bruce A. "Adipose Tissue-Derived Mesenchymal Stem Cells." Cells 10, no. 12 (December 6, 2021): 3433. http://dx.doi.org/10.3390/cells10123433.

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The long-held belief about adipose tissue was that it was relatively inert in terms of biological activity. It was believed that its primary role was energy storage; however, that was shattered with the discovery of adipokines. Scientists interested in regenerative medicine then reported that adipose tissue is rich in adult stromal/stem cells. Following these initial reports, adipose stem cells (ASCs) rapidly garnered interest for use as potential cellular therapies. The primary advantages of ASCs compared to other mesenchymal stem cells (MSCs) include the abundance of the tissue source for isolation, the ease of methodologies for tissue collection and cell isolation, and their therapeutic potential. Studies conducted both in vitro and in vivo have demonstrated that ASCs are multipotent, possessing the ability to differentiate into cells of mesodermal origins, including adipocytes, chondrocytes, osteoblast and others. Moreover, ASCs produce a broad array of cytokines, growth factors, nucleic acids (miRNAs), and other macromolecules into the surrounding milieu by secretion or in the context of microvesicles. The secretome of ASCs has been shown to alter tissue biology, stimulate tissue-resident stem cells, change immune cell activity, and mediate therapeutic outcomes. The quality of ASCs is subject to donor-to-donor variation driven by age, body mass index, disease status and possibly gender and ethnicity. This review discusses adipose stromal/stem cell action mechanisms and their potential utility as cellular therapeutics.

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Franco, GG, BW Minto, LP Coelho, PF Malard, ER Carvalho, FYK Kawamoto, BM Alcantara, and LGGG Dias. "Autologous adipose-derived mesenchymal stem cells and hydroxyapatite for bone defect in rabbits." Veterinární Medicína 67, No. 1 (November 29, 2021): 38–45. http://dx.doi.org/10.17221/85/2020-vetmed.

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This study aims to evaluate the effect of autologous adipose-derived mesenchymal stem cells (AAD-MSC), with and without synthetic absorbable hydroxyapatite (HAP-91), on the bone regeneration in rabbits. Thirty-four female white New Zealand rabbits were submitted to a 10 mm distal diaphyseal radius ostectomy, divided into 3 experimental groups according to the treatment established. The bone gap was filled with 0.15 ml of a 0.9% saline solution containing two million AAD-MSC (G1), or AAD-MSC associated with HAP-91 (G2). The control group (CG) received only 0.15 ml of the 0.9% saline solution. Radiographs were made post-operatively, and after 15, 30, 45 and 90 days. Fifty percent of the samples were submitted to a histological examination at 45 days and the remaining ones at 90 days post-operatively. Radiographically, the periosteal reaction, bone callus volume and bone bridge quality were superior in G2 (P < 0.05). Histologically, the bone repair was faster and more efficient in G1 at 45 days (P < 0.05). In conclusion, AAD-MSC improved the regeneration on the experimentally induced bone defects in rabbits; however, the use of hydroxyapatite requires caution given the granulomatous reaction produced in the species.

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Alió del Barrio, Jorge L., Ana De la Mata, María P. De Miguel, Francisco Arnalich-Montiel, Teresa Nieto-Miguel, Mona El Zarif, Marta Cadenas-Martín, et al. "Corneal Regeneration Using Adipose-Derived Mesenchymal Stem Cells." Cells 11, no. 16 (August 16, 2022): 2549. http://dx.doi.org/10.3390/cells11162549.

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Adipose-derived stem cells are a subtype of mesenchymal stem cell that offers the important advantage of being easily obtained (in an autologous manner) from low invasive procedures, rendering a high number of multipotent stem cells with the potential to differentiate into several cellular lineages, to show immunomodulatory properties, and to promote tissue regeneration by a paracrine action through the secretion of extracellular vesicles containing trophic factors. This secretome is currently being investigated as a potential source for a cell-free based regenerative therapy for human tissues, which would significantly reduce the involved costs, risks and law regulations, allowing for a broader application in real clinical practice. In the current article, we will review the existing preclinical and human clinical evidence regarding the use of such adipose-derived mesenchymal stem cells for the regeneration of the three main layers of the human cornea: the epithelium (derived from the surface ectoderm), the stroma (derived from the neural crest mesenchyme), and the endothelium (derived from the neural crest cells).

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8

Ko, M., TH Kim, Y. Kim, D. Kim, JO Ahn, BJ Kang, S. Choi, I. Park, JH Choi, and JY Chung. "Improvement of systemic lupus erythematosus in dogs with canine adipose-derived stem cells." Veterinární Medicína 64, No. 10 (October 26, 2019): 462–66. http://dx.doi.org/10.17221/46/2019-vetmed.

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A 6-year-old, intact female, Maltese presented with limited movement of the hind limbs and intermittent pruritus for three months. The patient was diagnosed with systemic lupus erythematosus. Conventional immunosuppressive therapy was attempted for 70 days; however, the patient still suffered from life-threatening pancreatitis and hepatopathy. Therefore, we tried canine adipose-derived mesenchymal stem cells for immunomodulation and liver protection. After 6-months of the stem cell therapy, the patient’s walking and hepatopathy improved. These findings indicate that stem cell therapy may be another option for systemic lupus erythematosus in dogs.

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9

Lee, Rebecca, Nicoletta Del Papa, Martin Introna, Charles F. Reese, Marina Zemskova, Michael Bonner, Gustavo Carmen-Lopez, Kristi Helke, Stanley Hoffman, and Elena Tourkina. "Adipose-derived mesenchymal stromal/stem cells in systemic sclerosis: Alterations in function and beneficial effect on lung fibrosis are regulated by caveolin-1." Journal of Scleroderma and Related Disorders 4, no. 2 (January 25, 2019): 127–36. http://dx.doi.org/10.1177/2397198318821510.

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The potential value of mesenchymal stromal/stem cell therapy in treating skin fibrosis in scleroderma (systemic sclerosis) and of the caveolin-1 scaffolding domain peptide in treating lung, skin, and heart fibrosis is known. To understand how these observations may relate to differences between mesenchymal stromal/stem cells from healthy subjects and subjects with fibrosis, we have characterized the fibrogenic and adipogenic potential of adipose-derived mesenchymal stromal/stem cells from systemic sclerosis patients, from mice with fibrotic lung and skin disease induced by systemic bleomycin treatment, and from healthy controls. Early passage systemic sclerosis adipose-derived mesenchymal stromal/stem cells have a profibrotic/anti-adipogenic phenotype compared to healthy adipose-derived mesenchymal stromal/stem cells (low caveolin-1, high α-smooth muscle actin, high HSP47, low pAKT, low capacity for adipogenic differentiation). This phenotype is mimicked by treating healthy adipose-derived mesenchymal stromal/stem cells with transforming growth factor beta or caveolin-1 small interfering RNA and is reversed in systemic sclerosis adipose-derived mesenchymal stromal/stem cells by treatment with caveolin-1 scaffolding domain peptide, but not scrambled caveolin-1 scaffolding domain peptide. Similar results were obtained with adipose-derived mesenchymal stromal/stem cells from systemic sclerosis patients and from bleomycin-treated mice, indicating the central role of caveolin-1 in mesenchymal stromal/stem cell differentiation in fibrotic disease.

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Hodgkinson, Tom, Francis Wignall, Judith A. Hoyland, and Stephen M. Richardson. "High BMPR2 expression leads to enhanced SMAD1/5/8 signalling and GDF6 responsiveness in human adipose-derived stem cells: implications for stem cell therapies for intervertebral disc degeneration." Journal of Tissue Engineering 11 (January 2020): 204173142091933. http://dx.doi.org/10.1177/2041731420919334.

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Stem cell–based regenerative strategies are promising for intervertebral disc degeneration. Stimulation of bone-marrow- and adipose-derived multipotent stem cells with recombinant human growth differentiation factor 6 (rhGDF6) promotes anabolic nucleus pulposus like phenotypes. In comparison to mesenchymal stem cells, adipose-derived multipotent stem cells exhibit greater NP-marker gene expression and proteoglycan-rich matrix production. To understand these response differences, we investigated bone morphogenetic protein receptor profiles in donor-matched human mesenchymal stem cells and adipose-derived multipotent stem cells, determined differences in rhGDF6 signalling and their importance in NP-like differentiation between cell populations. Bone morphogenetic protein receptor expression in mesenchymal stem cells and adipose-derived multipotent stem cells revealed elevated and less variable expression of BMPR2 in adipose-derived multipotent stem cells, which corresponded with increased downstream pathway activation (SMAD1/5/8, ERK1/2). Inhibitor studies demonstrated SMAD1/5/8 signalling was required for rhGDF6-induced nucleus-pulposus-like adipose-derived multipotent stem cell differentiation, while ERK1/2 contributed significantly to critical nucleus pulposus gene expression, aggrecan and type II collagen production. These data inform cell regenerative therapeutic choices for intervertebral disc degeneration regeneration and identify further potential optimisation targets.

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11

Wang, Jingkai, Yiqing Tao, Xiaopeng Zhou, Hao Li, Chengzhen Liang, Fangcai Li, and Qi-xin Chen. "The potential of chondrogenic pre-differentiation of adipose-derived mesenchymal stem cells for regeneration in harsh nucleus pulposus microenvironment." Experimental Biology and Medicine 241, no. 18 (August 19, 2016): 2104–11. http://dx.doi.org/10.1177/1535370216662362.

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Recent studies indicated that cell-based therapy could be a promising approach to treat intervertebral disc degeneration. Though the harsh microenvironment in disc is still challenging to implanted cells, it could be overcome by pre-conditioning graft cells before transplantation, suggested by previous literatures. Therefore, we designed this study to identify the potential effect of chondrogenic pre-differentiation on adipose-derived mesenchymal stem cells in intervertebral disc-like microenvironment, characterized by limited nutrition, acidic, and high osmosis in vitro. Adipose-derived mesenchymal stem cells of rat were divided into five groups, embedded in type II collagen scaffold, and cultured in chondrogenic differentiation medium for 0, 3, 7, 10, and 14 days. Then, the adipose-derived mesenchymal stem cells were implanted and cultured in intervertebral disc-like condition. The proliferation and differentiation of adipose-derived mesenchymal stem cells were evaluated by cell counting kit-8 test, real-time quantitative polymerase chain reaction, and Western blotting and immunofluorescence analysis. Analyzed by the first week in intervertebral disc-like condition, the results showed relatively greater proliferative capability and extracellular matrix synthesis ability of the adipose-derived mesenchymal stem cells pre-differentiated for 7 and 10 days than the control. We concluded that pre-differentiation of rat adipose-derived mesenchymal stem cells in chondrogenic culture medium for 7 to 10 days could promote the regeneration effect of adipose-derived mesenchymal stem cells in intervertebral disc-like condition, and the pre-differentiated cells could be a promising cell source for disc regeneration medicine.

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12

Baer, Patrick C. "Adipose-Derived Stromal/Stem Cells." Cells 9, no. 9 (August 30, 2020): 1997. http://dx.doi.org/10.3390/cells9091997.

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13

Palencar, D., J. Dragunova, I. Hulin, and J. Koller. "Adipose derived mesenchymal stem cells harvesting." Bratislava Medical Journal 120, no. 09 (2019): 686–89. http://dx.doi.org/10.4149/bll_2019_115.

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14

Koplay, Tugba Gun, Gokce Yildiran, Duygu Dursunoglu, Murad Aktan, Selcuk Duman, Osman Akdag, Mehtap Karamese, and Zekeriya Tosun. "The Effects of Adipose-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cell–Originating Exosomes on Nerve Allograft Regeneration." Annals of Plastic Surgery 90, no. 3 (March 2023): 261–66. http://dx.doi.org/10.1097/sap.0000000000003414.

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15

Mori, Daisuke, Shigeru Miyagawa, Takuji Kawamura, Daisuke Yoshioka, Hiroki Hata, Takayoshi Ueno, Koichi Toda, et al. "Mitochondrial Transfer Induced by Adipose-Derived Mesenchymal Stem Cell Transplantation Improves Cardiac Function in Rat Models of Ischemic Cardiomyopathy." Cell Transplantation 32 (January 2023): 096368972211484. http://dx.doi.org/10.1177/09636897221148457.

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Although mesenchymal stem cell transplantation has been successful in the treatment of ischemic cardiomyopathy, the underlying mechanisms remain unclear. Herein, we investigated whether mitochondrial transfer could explain the success of cell therapy in ischemic cardiomyopathy. Mitochondrial transfer in co-cultures of human adipose-derived mesenchymal stem cells and rat cardiomyocytes maintained under hypoxic conditions was examined. Functional recovery was monitored in a rat model of myocardial infarction following human adipose-derived mesenchymal stem cell transplantation. We observed mitochondrial transfer in vitro, which required the formation of cell-to-cell contacts and synergistically enhanced energy metabolism. Rat cardiomyocytes exhibited mitochondrial transfer 3 days following human adipose-derived mesenchymal stem cell transplantation to the ischemic heart surface post-myocardial infarction. We detected donor mitochondrial DNA in the recipient myocardium concomitant with a significant improvement in cardiac function. Mitochondrial transfer is vital for successful cell transplantation therapies and improves treatment outcomes in ischemic cardiomyopathy.

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El-Sayyad, HIH, MA Sobh, SA Khalifa, and OKRA El-Sayyad. "Adipose Derived Mesenchymal Stem Cell Differentiation into Adipogenic and Osteogenic Stem Cells." Studies on Stem Cells Research and Therapy 2, no. 1 (December 29, 2016): 025–32. http://dx.doi.org/10.17352/sscrt.000008.

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17

Bräunig, P., W. G. Glanzner, V. B. Rissi, and P. B. D. Gonçalves. "The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 70, no. 1 (January 2018): 160–68. http://dx.doi.org/10.1590/1678-4162-9132.

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ABSTRACT The adipose tissue is a reliable source of Mesenchymal stem cells (MSCs) showing a higher plasticity and transdifferentiation potential into multilineage cells. In the present study, adipose tissue-derived mesenchymal stem cells (AT-MSCs) were isolated from mice omentum and epididymis fat depots. The AT-MSCs were initially compared based on stem cell surface markers and on the mesodermal trilineage differentiation potential. Additionally, AT-MSCs, from both sources, were cultured with differentiation media containing retinoic acid (RA) and/or testicular cell-conditioned medium (TCC). The AT-MSCs expressed mesenchymal surface markers and differentiated into adipogenic, chondrogenic and osteogenic lineages. Only omentum-derived AT-MSCs expressed one important gene marker related to male germ cell lineages, after the differentiation treatment with RA. These findings reaffirm the importance of adipose tissue as a source of multipotent stromal-stem cells, as well as, MSCs source regarding differentiation purpose.

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Widera, Darius. "Recent Advances in Translational Adipose-Derived Stem Cell Biology." Biomolecules 11, no. 11 (November 9, 2021): 1660. http://dx.doi.org/10.3390/biom11111660.

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Lee, Eun-Sun, Eun-A. Kwon, Jeong-Ran Park, Byung-Chul Kang, Kyung-Sun Kang, and Myung-Haing Cho. "Tumorigenesis Study of Canine Adipose Derived-mesenchymal Stem Cell." Toxicological Research 23, no. 3 (September 30, 2007): 271–78. http://dx.doi.org/10.5487/tr.2007.23.3.271.

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20

Kunze, Kyle N., Robert A. Burnett, Joshua Wright-Chisem, Rachel M. Frank, and Jorge Chahla. "Adipose-Derived Mesenchymal Stem Cell Treatments and Available Formulations." Current Reviews in Musculoskeletal Medicine 13, no. 3 (April 23, 2020): 264–80. http://dx.doi.org/10.1007/s12178-020-09624-0.

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21

Jang, Jun Ho, Hyun Woo Lee, Young-Woo Eom, Seok Yun Kang, Joon Seong Park, Jin Hyuk Choi, and Hugh C. Kim. "Characteristics of Adipose Tissue-Derived Mesenchymal Stem Cells." Blood 108, no. 11 (November 16, 2006): 4251. http://dx.doi.org/10.1182/blood.v108.11.4251.4251.

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Abstract Background: The aim of this study was to examine the differentiation potentials and characteristics of adipose tissue-derived stem cells (ASCs) in other to evaluate for the ASCs to be used as alternative cell sources of mesenchymal stem cells. Methods: ASCs were isolated from lipo-aspirated adipose tissues by treatment of collagenase A and cultured in a Dulbecco’s Modified Eagle’s Medium (DMEM). To know the characteristics of ASCs, the expression of cell surface antigens was analyzed by flow cytometry, and the proliferation potentials were estimated by colony forming abilities or capacities of population doubling. The differentiation potentials into adipocytes or osteoblasts were confirmed by accumulation of neutral lipid vacuoles stained with Oil-red O and expression of alkaline phosphatases. Results: When the nucleated cells were isolated by collagenase treatment after lipo-aspiration, the mean cell yield was about 3.1 X 106 or 1.2 X 106 cells per gram of lipo-aspirate (n=8) processed at 3 or 21 hours, respectively. But cells processed at 21 hours were able to form more colonies than those at 3 hours. Flowcytometric analysis showed that Adipose tissue-derived stem cells (ASCs) have a marker expression that is similar to that of bone marrow stromal cells (BMSCs). ASCs expressed CD44, CD73, CD90, and CD105 and were absent for CD14, CD31 and CD45 expression. When primary cells were plated at 50 or 1000 cells/cm2 on 6-well plates, cumulative population doublings were about 50 times until passage 7 or 13 (approximately 130 days), respectively, and ASCs expanded to 1018 cells. ASCs were multipotent, differentiating to the adipocyte and osteoblast lineages. ASCs did not provoke in vitro alloreactivity of incompatable lymphocytes and, moreover, suppressed mixed lymphocyte reaction (MLR) and lymphocyte proliferative response to mitogen. Conclusion: Our results suggested that ASCs have highly proliferative potentials, multiple differentiation potentials, and immunosuppressive properties like BMSCs. Therefore, ASCs-based reconstructive therapy could employ allogeneic cells and because of their immunosuppressive properties, ASCs could be an alternative source of BMSCs to treat allogeneic conflicts.

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Konno, Masamitsu, Atsushi Hamabe, Shinichiro Hasegawa, Hisataka Ogawa, Takahito Fukusumi, Shimpei Nishikawa, Katsuya Ohta, et al. "Adipose-derived mesenchymal stem cells and regenerative medicine." Development, Growth & Differentiation 55, no. 3 (March 3, 2013): 309–18. http://dx.doi.org/10.1111/dgd.12049.

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Miyagi-Shiohira, Chika, Kiyoto Kurima, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, and Hirofumi Noguchi. "Cryopreservation of Adipose-Derived Mesenchymal Stem Cells." Cell Medicine 8, no. 1-2 (August 2015): 3–7. http://dx.doi.org/10.3727/215517915x689100.

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Surowiecka, Agnieszka, and Jerzy Strużyna. "Adipose-Derived Stem Cells for Facial Rejuvenation." Journal of Personalized Medicine 12, no. 1 (January 16, 2022): 117. http://dx.doi.org/10.3390/jpm12010117.

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The interest in regenerative medicine is increasing, and it is a dynamically developing branch of aesthetic surgery. Biocompatible and autologous-derived products such as platelet-rich plasma or adult mesenchymal stem cells are often used for aesthetic purposes. Their application originates from wound healing and orthopaedics. Adipose-derived stem cells are a powerful agent in skin rejuvenation. They secrete growth factors and anti-inflammatory cytokines, stimulate tissue regeneration by promoting the secretion of extracellular proteins and secrete antioxidants that neutralize free radicals. In an office procedure, without cell incubation and counting, the obtained product is stromal vascular fraction, which consists of not only stem cells but also other numerous active cells such as pericytes, preadipocytes, immune cells, and extra-cellular matrix. Adipose-derived stem cells, when injected into dermis, improved skin density and overall skin appearance, and increased skin hydration and number of capillary vessels. The main limitation of mesenchymal stem cell transfers is the survival of the graft. The final outcomes are dependent on many factors, including the age of the patient, technique of fat tissue harvesting, technique of lipoaspirate preparation, and technique of fat graft injection. It is very difficult to compare available studies because of the differences and multitude of techniques used. Fat harvesting is associated with potentially life-threatening complications, such as massive bleeding, embolism, or clots. However, most of the side effects are mild and transient: primarily hematomas, oedema, and mild pain. Mesenchymal stem cells that do not proliferate when injected into dermis promote neoangiogenesis, that is why respectful caution should be taken in the case of oncologic patients. A longer clinical observation on a higher number of participants should be performed to develop reliable indications and guidelines for transferring ADSCs.

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Yasumura, Yuyo, Takahiro Teshima, Tomokazu Nagashima, Takashi Takano, Masaki Michishita, Yoshiaki Taira, Ryohei Suzuki, and Hirotaka Matsumoto. "Immortalized Canine Adipose-Derived Mesenchymal Stem Cells as a Novel Candidate Cell Source for Mesenchymal Stem Cell Therapy." International Journal of Molecular Sciences 24, no. 3 (January 23, 2023): 2250. http://dx.doi.org/10.3390/ijms24032250.

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Mesenchymal stem cells are expected to be a cell source for stem cell therapy of various diseases in veterinary medicine. However, donor-dependent cell heterogenicity has been a cause of inconsistent therapeutic efficiency. Therefore, we established immortalized cells from canine adipose tissue-derived mesenchymal stem cells (ADSCs) to minimize cellular heterogeneity by reducing the number of donors, evaluated their properties, and compared them to the primary cells with RNA-sequencing. Immortalized canine ADSCs were established by transduction with combinations of the R24C mutation of human cyclin-dependent kinase 4 (CDKR24C), canine cyclin D1, and canine TERT. The ADSCs transduced with CDK4R24C, cyclin D1, and TERT (ADSC-K4DT) or with CDK4R24C and cyclin D1 (ADSC-K4D) showed a dramatic increase in proliferation (population doubling level >100) without cellular senescence compared to the primary ADSCs. The cell surface markers, except for CD90 of the ADSC-K4DT and ADSC-K4D cells, were similar to those of the primary ADSCs. The ADSC-K4DT and ADSC-K4D cells maintained their trilineage differentiation capacity and chromosome condition, and did not have a tumorigenic development. The ability to inhibit lymphocyte proliferation by the ADSC-K4D cells was enhanced compared with the primary ADSCs and ADSC-K4DT cells. The pathway analysis based on RNA-sequencing revealed changes in the pathways mainly related to the cell cycle and telomerase. The ADSC-K4DT and ADSC-K4D cells had decreased CD90 expression, but there were no obvious defects associated with the decreased CD90 expression in this study. Our results suggest that ADSC-K4DT and ADSC-K4D cells are a potential novel cell source for mesenchymal stem cell therapy.

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Moorman, Claude T., Kwadwo A. Owusu-Akyaw, Jonathan Godin, Stefano Pecchia, and Alexander Oldweiler. "Adipose-derived Mesenchymal Stem Cells and Arthroscopic Surgery." Duke Orthopaedic Journal 7, no. 1 (2017): 34–38. http://dx.doi.org/10.5005/jp-journals-10017-1079.

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ABSTRACT Mesenchymal stem cells (MSCs) are multipotent cells with potential reparative properties for connective tissues, such as articular cartilage. The Lipogems adipose graft harvest system is a relatively novel technique for harvesting adipose-derived MSCs and may be utilized in conjunction with various orthopaedic sports medicine procedures. Owusu-Akyaw KA, Godin J, Pecchia S, Oldweiler A, Moorman CT. Adipose-derived Mesenchymal Stem Cells and Arthroscopic Surgery. The Duke Orthop J 2017;7(1):34-38.

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Airuddin, Siti Syahira, Ahmad Sukari Halim, Wan Azman Wan Sulaiman, Ramlah Kadir, and Nur Azida Mohd Nasir. "Adipose-Derived Stem Cell: “Treat or Trick”." Biomedicines 9, no. 11 (November 5, 2021): 1624. http://dx.doi.org/10.3390/biomedicines9111624.

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Stem cells have been widely used for treating disease due to the various benefits they offer in the curing process. Several treatments using stem cells have undergone clinical trials, such as cell-based therapies for heart disease, sickle cell disease, thalassemia, etc. Adipose-derived stem cells are some of the many mesenchymal stem cells that exist in our body that can be harvested from the abdomen, thighs, etc. Adipose tissue is easy to harvest, and its stem cells can be obtained in higher volumes compared to stem cells harvested from bone marrow, for which a more invasive technique is required with a smaller volume obtained. Many scientists have expressed interest in investigating the role of adipose-derived stem cells in treating disease since their use was first described. This is due to these stem cells’ ability to differentiate into multiple lineages and secrete a variety of growth factors and proteins. Previous studies have found that the hormones, cytokines, and growth factors contained in adipose tissue play major roles in the metabolic regulation of adipose tissue, as well as in energy balance and whole-body homeostasis through their endocrine, autocrine, and paracrine functions. These are thought to be important contributors to the process of tissue repair and regeneration. However, it remains unclear how effective and safe ADSCs are in treating diseases. The research that has been carried out to date is in order to investigate the impact of ADSCs in disease treatment, as described in this review, to highlight its “trick or treat” effect in medical treatment.

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Ahmed Elkammar, Hala. "Effect of human bone marrow derived mesenchymal stem cells on squamous cell carcinoma cell line." International Journal of Academic Research 6, no. 1 (January 30, 2014): 110–16. http://dx.doi.org/10.7813/2075-4124.2014/6-1/a.14.

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Qu, Rongmei, Kai He, Tingyu Fan, Yuchao Yang, Liyao Mai, Zhiwei Lian, Zhitao Zhou, et al. "Single-Cell Transcriptomic Sequencing Analyses of Cell Heterogeneity During Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells." Stem Cells 39, no. 11 (August 16, 2021): 1478–88. http://dx.doi.org/10.1002/stem.3442.

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Abstract Mesenchymal stem cells (MSCs) are known for their multilineage differentiation potential with immune-modulatory properties. The molecular underpinnings of differentiation remain largely undefined. In this study, we investigated the cellular and molecular features of chemically induced osteogenesis from MSC isolated from human adipose tissue (human adipose MSCs, hAMSCs) using single-cell RNA-sequencing (scRNA-seq). We found that a near complete differentiation of osteogenic clusters from hAMSCs under a directional induction. Both groups of cells are heterogeneous, and some of the hAMSCs cells are intrinsically prepared for osteogenesis, while variant OS clusters seems in cooperation with a due division of the general function. We identified a set of genes related to cell stress response highly expressed during the differentiation. We also characterized a series of transitional transcriptional waves throughout the process from hAMSCs to osteoblast and specified the unique gene networks and epigenetic status as key markers of osteogenesis.

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Thangarajah, Hariharan, I. Nick Vial, Edwin Chang, Samyra El-ftesi, Edward I. Chang, Kirit Bhatt, Josemaria Paterno, and Geoffrey C. Gurtner. "Hypoxic regulation of proangiogenic adipose-derived mesenchymal stem cell function." Journal of the American College of Surgeons 207, no. 3 (September 2008): S69. http://dx.doi.org/10.1016/j.jamcollsurg.2008.06.172.

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Li, Hong, Bin Zhang, Yuanqing Lu, Marda Jorgensen, Bryon Petersen, and Sihong Song. "Adipose tissue-derived mesenchymal stem cell-based liver gene delivery." Journal of Hepatology 54, no. 5 (May 2011): 930–38. http://dx.doi.org/10.1016/j.jhep.2010.07.051.

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Lo Furno, Debora, Giuliana Mannino, Venera Cardile, Rosalba Parenti, and Rosario Giuffrida. "Potential Therapeutic Applications of Adipose-Derived Mesenchymal Stem Cells." Stem Cells and Development 25, no. 21 (November 2016): 1615–28. http://dx.doi.org/10.1089/scd.2016.0135.

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Wei, Yudong, Jia Fang, Shufang Cai, Changrong Lv, Shiqiang Zhang, and Jinlian Hua. "Primordial germ cell-like cells derived from canine adipose mesenchymal stem cells." Cell Proliferation 49, no. 4 (July 4, 2016): 503–11. http://dx.doi.org/10.1111/cpr.12271.

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Miyagi-Shiohira, Chika, Yoshiki Nakashima, Naoya Kobayashi, Shinji Kitamura, Issei Saitoh, Masami Watanabe, and Hirofumi Noguchi. "Induction of Expandable Adipose-Derived Mesenchymal Stem Cells from Aged Mesenchymal Stem Cells by a Synthetic Self-Replicating RNA." International Journal of Molecular Sciences 19, no. 11 (November 6, 2018): 3489. http://dx.doi.org/10.3390/ijms19113489.

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Adipose-derived mesenchymal stem cells (ADSCs) have attracted attention due to their potential for use in the treatment of various diseases. However, the self-renewal capacity of ADSCs is restricted and their function diminishes during passage. We previously generated induced tissue-specific stem cells from mouse pancreatic cells using a single synthetic self-replicating Venezuelan Equine Encephalitis (VEE)-reprogramming factor (RF) RNA replicon (SR-RNA) expressing the reprogramming factors POU class 5 homeobox 1 (OCT4), Krueppel-like factor 4 (KLF4), Sex determining region Y-box 2 (SOX2), and Glis Family Zinc Finger 1 (GLIS1). This vector was used to generate induced pluripotent stem (iPS) cells. Here, we applied this SR-RNA vector to generate human iTS cells from aged mesenchymal stem cells (hiTS-M cells) deficient in self-renewal that were derived from adipose tissue. These hiTS-M cells transfected with the SR-RNA vector survived for 15 passages. The hiTS-M cells expressed cell surface markers similar to those of human adipose-derived mesenchymal stem cells (hADSCs) and differentiated into fat cells and osteoblasts. Global gene expression profiling showed that hiTS-M cells were transcriptionally similar to hADSCs. These data suggest that the generation of iTS cells has important implications for the clinical application of autologous stem cell transplantation.

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Kaya, Alper, Edip Yılmaz, and Berhan Bayram. "Mesenchymal stem cell applications in chondral lesions: Bone marrow and adipose derived stem cells." TOTBİD Dergisi 22, no. 2 (March 15, 2023): 101–7. http://dx.doi.org/10.5578/totbid.dergisi.2023.16.

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Fernandes, Marcela, MariaJosé da Silva Fernandes, SandraGomes Valente, RodrigoGuerra Sabongi, JoãoBaptista Gomes dos Santos, VilneiMattioli Leite, Henning Ulrich, and ArthurAndrade Nery. "Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration." Neural Regeneration Research 13, no. 1 (2018): 100. http://dx.doi.org/10.4103/1673-5374.224378.

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Irioda, Ana Carolina, Rafael Cassilha, Larissa Zocche, Julio Cesar Francisco, Ricardo Correa Cunha, Priscila Elias Ferreira, Luiz Cesar Guarita-Souza, et al. "Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decreaseα4-Integrin Expression." Stem Cells International 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/2562718.

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Aim. The effects of cryopreservation on adipose tissue-derived mesenchymal stem cells are not clearly documented, as there is a growing body of evidence about the importance of adipose-derived mesenchymal stem cells for regenerative therapies. The aim of this study was to analyze human adipose tissue-derived mesenchymal stem cells phenotypic expression (CD34, CD45, CD73, CD90, CD105, and CD49d), colony forming unit ability, viability, and differentiation potential before and after cryopreservation.Materials and Methods. 12 samples of the adipose tissue were collected from a healthy donor using the liposuction technique. The cell isolation was performed by enzymatic digestion and then the cells were cultured up to passage 2. Before and after cryopreservation the immunophenotype, cellular viability analysis by flow cytometer, colony forming units ability, differentiation potential into adipocytes and osteoblasts as demonstrated by Oil Red O and Alizarin Red staining, respectively.Results. The immunophenotypic markers expression was largely preserved, and their multipotency was maintained. However, after cryopreservation, the cells decreasedα4-integrin expression (CD49d), cell viability, and number of colony forming units.Conclusions. These findings suggest that ADMSC transplanted after cryopreservation might compromise the retention of transplanted cells in the host tissue. Therefore, further studies are warranted to standardize protocols related to cryopreservation to attain full benefits of stem cell therapy.

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Gaiba, Silvana, Lucimar Pereira de França, Jerônimo Pereira de França, and Lydia Masako Ferreira. "Characterization of human adipose-derived stem cells." Acta Cirurgica Brasileira 27, no. 7 (July 2012): 471–76. http://dx.doi.org/10.1590/s0102-86502012000700007.

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PURPOSE: There is a growing scientific interest in the plasticity and therapeutic potential of adipose-derived stem cells (ASCs), which are multipotent and abundant in adipose tissue and can differentiate in vitro into multiple lineages, including adipocytes, chondrocytes, osteoblasts, neural cells, endothelial cells and cardiomyocytes. The aim of this study was to isolate, cultivate and identify ASCs. METHODS: Human adipose precursor cells were obtained from subcutaneous abdominal tissue. Recently dispersed cells were separated by density centrifugation gradient, cultured and then analyzed. RESULTS: Human ASCs were able to replicate in our culture conditions. The cells maintained their phenotypes throughout the studied period on different passages confirming they suitability for in vitro cultivation. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. Flow cytometry results showed that these cells expressed CD73, CD90 and CD105, (mesenchymal stem-cells markers), contrasting with the lack of expression of CD16, CD34 and CD45 (hematopoietic cells markers). CONCLUSION: It was possible to isolate human adipose-derived stem cells by in vitro cultivation without adipogenic induction, maintaining their functional integrity and high proliferation levels. The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro.

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Crop, Meindert J., Carla C. Baan, Sander S. Korevaar, Jan N. M. Ijzermans, Willem Weimar, and Martin J. Hoogduijn. "Human Adipose Tissue-Derived Mesenchymal Stem Cells Induce Explosive T-Cell Proliferation." Stem Cells and Development 19, no. 12 (December 2010): 1843–53. http://dx.doi.org/10.1089/scd.2009.0368.

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Radtke, C., B. Schmitz, M. Spies, J. D. Kocsis, and P. M. Vogt. "Peripheral glial cell differentiation from neurospheres derived from adipose mesenchymal stem cells." International Journal of Developmental Neuroscience 27, no. 8 (August 21, 2009): 817–23. http://dx.doi.org/10.1016/j.ijdevneu.2009.08.006.

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Radtke, C., B. Schmitz, M. Spies, J. Kocsis, and P. M. Vogt. "OP26: OLFACTORY ENSHEATHING CELL-LIKE DIFFERENTIATION OF ADIPOSE-DERIVED MESENCHYMAL STEM CELLS." Plastic and Reconstructive Surgery 124, Supplement (August 2009): 688. http://dx.doi.org/10.1097/01.prs.0000358930.01838.62.

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Zhou, Lingcong, Hui Wang, Sidi Yao, Li Li, and Xin Kuang. "Efficacy of Human Adipose Derived Mesenchymal Stem Cells in Promoting Skin Wound Healing." Journal of Healthcare Engineering 2022 (March 24, 2022): 1–5. http://dx.doi.org/10.1155/2022/6590025.

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Background. The aim of this pilot clinical study is to evaluate the efficacy of human adipose derived mesenchymal stem cells (HAMSCs) treatment for the wound healing with patients. Methods. This study was a clinical trial to investigate the efficacy of human adipose derived mesenchymal stem cells treatment for the wound healing with patients. 346 patients with skin wounds attending the central hospital of Yue Yang were enrolled in the study, setting in the period from January 2016 to January 2021. Patients were randomly allocated into two groups: experimental group received treatment with human adipose derived mesenchymal stem cells for each 10 cm2 of wound and control group received conventional dressing with normal saline for each 10 cm2 of wound. Results. No adverse events were recorded during the period of treatment. The granulation tissue coverage rate and thickness of granulation tissue after 10 days of treatment in experimental group were significantly improved compared with control group. Furthermore, the occurrence of bleeding of wound and suppurative wounds between two groups had significant difference ( P < 0.05 ). Conclusion. The data in this pilot study indicated that human adipose derived mesenchymal stem cells may be a safe and effective alternative therapy for wound healing. Moreover, larger, placebo-controlled, perspective studies are necessity to evaluate the efficacy and safety of human adipose derived mesenchymal stem cells treatment for wound healing patients.

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Liu, Haihui, Mingtai Chen, Lulu Liu, Saisai Ren, Panpan Cheng, and Hao Zhang. "Induction of Human Adipose-Derived Mesenchymal Stem Cells into Germ Lineage Using Retinoic Acid." Cellular Reprogramming 20, no. 2 (April 2018): 127–34. http://dx.doi.org/10.1089/cell.2017.0063.

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Constantin, Gabriela, Silvia Marconi, Barbara Rossi, Stefano Angiari, Laura Calderan, Elena Anghileri, Beatrice Gini, et al. "Adipose-Derived Mesenchymal Stem Cells Ameliorate Chronic Experimental Autoimmune Encephalomyelitis." Stem Cells 27, no. 10 (October 2009): 2624–35. http://dx.doi.org/10.1002/stem.194.

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Carrera-Arrabal, Thaiz, José Luis Calvo-Guirado, Fabricio Passador-Santos, Carlos Eduardo Sorgi da Costa, Frank Róger Teles Costa, Antonio Carlos Aloise, Marcelo Henrique Napimoga, Juan Manuel Aragoneses, and André Antonio Pelegrine. "Vertical Bone Construction with Bone Marrow-Derived and Adipose Tissue-Derived Stem Cells." Symmetry 11, no. 1 (January 8, 2019): 59. http://dx.doi.org/10.3390/sym11010059.

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The purpose of this study was to conduct a histomorphometric analysis of bone marrow-derived and adipose tissue-derived stem cells, associated with a xenograft block, in vertical bone constructions in rabbit calvaria. Ten rabbits received two xenograft blocks on the calvaria, after decortication of the parietal bone. The blocks were fixed with titanium screws. The blocks were combined with the bone marrow-derived mesenchymal stem cells in the bone marrow stem cell (BMSC) group (right side of the calvaria) or with the adipose tissue-derived mesenchymal stem cells in the adipose tissue stem cell (ATSC) group (left side of the calvaria). After 8 weeks, the animals were sacrificed and their parietal bones were fixed in 10% formalin for the histomorphometric analysis. The following parameters were evaluated—newly formed bone (NFB), xenogeneic residual particles (XRP), and non-mineralized tissue (NMT). The histomorphometric analysis revealed 11.9 ± 7.5% and 7.6 ± 5.6% for NFB, 22.14 ± 8.5% and 21.6 ± 8.5% for XRP, and 65.8 ± 10.4% and 70.8 ± 7.4% for NMT in groups BMSC and ATSC, respectively, with statistically significant differences in the NFB and the NMT between the groups, but no differences in the XRP. Therefore, it can be concluded that the bone marrow-derived stem cells seem to have more potential for the bone formation than do the adipose tissue-derived stem cells when used in combination with the xenogenous blocks in the vertical bone construction.

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Fiedler, Tomas, Magdalena Rabe, Ralf G. Mundkowski, Sonja Oehmcke-Hecht, and Kirsten Peters. "Adipose-derived mesenchymal stem cells release microvesicles with procoagulant activity." International Journal of Biochemistry & Cell Biology 100 (July 2018): 49–53. http://dx.doi.org/10.1016/j.biocel.2018.05.008.

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Skubis, Aleksandra, Bartosz Sikora, Nikola Zmarzły, Emilia Wojdas, and Urszula Mazurek. "Adipose-derived stem cells: a review of osteogenesis differentiation." Folia Biologica et Oecologica 12 (December 7, 2016): 38–47. http://dx.doi.org/10.1515/fobio-2016-0004.

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This review article provides an overview on adipose-derived stem cells (ADSCs) for implications in bone tissue regeneration. Firstly this article focuses on mesenchymal stem cells (MSCs) which are object of interest in regenerative medicine. Stem cells have unlimited potential for self-renewal and develop into various cell types. They are used for many therapies such as bone tissue regeneration. Adipose tissue is one of the main sources of mesenchymal stem cells (MSCs). Regenerative medicine intends to differentiate ADSC along specific lineage pathways to effect repair of damaged or failing organs. For further clinical applications it is necessary to understand mechanisms involved in ADSCs proliferation and differentiation. Second part of manuscript based on osteogenesis differentiation of stem cells. Bones are highly regenerative organs but there are still many problems with therapy of large bone defects. Sometimes there is necessary to make a replacement or expansion new bone tissue. Stem cells might be a good solution for this especially ADSCs which manage differentiate into osteoblast in in vitro and in vivo conditions.

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Kim, Jeong Mi, Mi Eun Choi, Eun Jeong Jeon, Jin-Mi Park, Sungryeal Kim, Jeong Eun Park, Seung Wook Oh, and Jeong-Seok Choi. "Cell-derived vesicles from adipose-derived mesenchymal stem cells ameliorate irradiation-induced salivary gland cell damage." Regenerative Therapy 21 (December 2022): 453–59. http://dx.doi.org/10.1016/j.reth.2022.09.007.

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Barboza, Carlos Augusto Galvão, Fernanda Ginani, Diego Moura Soares, Águida Cristina Gomes Henriques, and Roseana de Almeida Freitas. "Low-level laser irradiation induces in vitro proliferation of mesenchymal stem cells." Einstein (São Paulo) 12, no. 1 (March 2014): 75–81. http://dx.doi.org/10.1590/s1679-45082014ao2824.

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Objective : To evaluate the effect of low-level laser irradiation on the proliferation and possible nuclear morphological changes of mouse mesenchymal stem cells. Methods : Mesenchymal stem cells derived from bone marrow and adipose tissue were submitted to two applications (T0 and T48 hours) of low-level laser irradiation (660nm; doses of 0.5 and 1.0J/cm2). The trypan blue assay was used to evaluate cell viability, and growth curves were used to analyze proliferation at zero, 24, 48, and 72 hours. Nuclear alterations were evaluated by staining with DAPI (4’-6-diamidino-2-phenylindole) at 72 hours. Results : Bone marrow-derived mesenchymal stem cells responded to laser therapy in a dose-dependent manner. Higher cell growth was observed when the cells were irradiated with a dose of 1.0J/cm2, especially after 24 hours (p<0.01). Adipose-derived mesenchymal stem cells responded better to a dose of 1.0J/cm2, but higher cell proliferation was observed after 48 hours (p<0.05) and 72 hours (p<0.01). Neither nuclear alterations nor a significant change in cell viability was detected in the studied groups. Conclusion : Low-level laser irradiation stimulated the proliferation of mouse mesenchymal stem cells without causing nuclear alterations. The biostimulation of mesenchymal stem cells using laser therapy might be an important tool for regenerative therapy and tissue engineering.

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Lina, Yani, and Andi Wijaya. "Adipose-Derived Stem Cells for Future Regenerative System Medicine." Indonesian Biomedical Journal 4, no. 2 (August 1, 2012): 59. http://dx.doi.org/10.18585/inabj.v4i2.164.

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BACKGROUND: The potential use of stem cell-based therapies for repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions for a number of disease. Despite the advances, the availability of stem cells remaining a challenge for both scientist and clinicians in pursuing regenerative medicine. CONTENT: Subcutaneous human adipose tissue is an abundant and accessible cell source for applications in tissue engineering and regenerative medicine. Routinely, the adipose issue is digested with collagenase or related lytic enzymes to release a heterogeneous population for stromal vascular fraction (SVF) cells. The SVF cells can be used directly or can be cultured in plastic ware for selection and expansion of an adherent population known as adipose-derived stromal/stem cells (ASCs). Their potential in the ability to differentiate into adipogenic, osteogenic, chondrogenic and other mesenchymal lineages, as well in their other clinically useful properties, includes stimulation of angiogenesis and suppression of inflammation.SUMMARY: Adipose tissue is now recognized as an accessible, abundant and reliable site for the isolation of adult stem cels suitable for the application of tissue engineering and regenerative medicine applications. The past decade has witnessed an explosion of preclinical data relating to the isolation, characterization, cryopreservation, differentiation, and transplantation of freshly isolated stromal vascular fraction cells and adherent, culture-expanded, adipose-derived stromal/stem cells in vitro and in animal models.KEYWORDS: adipose tissue, adult stem cells, regenerative medicine, mesenchymal stem cells

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