Dissertations / Theses on the topic 'Adipogensi'
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AL, HAJ GHINA. "EFFECTS OF LIPID MIXTURE AND A SELECTIVE PPARG MODULATOR ON THE DIFFERENTIATION CAPABILITIES OF HUMAN DERIVED MESENCHYMAL STEM CELLS(HADSCS) DERIVED FROM HEALTHY AN D BREAST CANCER PATIENTS." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/784157.
Full textMetabolic syndrome is associated with many complications especially leading to life threatening disorders such as obesity and cancer. To be able to identify solutions and natural treatments, we need to investigate the underlying causes of this syndrome. Nutrition is one important factor to consider in the prevention and treatment of the metabolic syndrome. Nutrition effects almost all metabolism mechanisms in the human body. One provident effect of nutrition is adiposity. Over the recent years, an interest was noted to studying adipogenesis in relation to obesity. Different factors affect adipogenesis including natural dietary compounds to help decrease adiposity, therefore the risk of developing obesity and later on obesity related diseases such as breast cancer. To be able to study this correlation in-vitro, a wide choice of cell models can be used. Human adipose derived mesenchymal cells (hADSCs) are one of the top choices used to study adipogenesis overcoming the limitations that other cell models have in their applicability to humans regarding the prevailing difference in their metabolism and physiology. In this study, the aim was to study adipogenesis using hADSCs in presence of dietary compounds such as lipids and GMG-43AC, a natural selective peroxisome proliferator-activated receptor g (PPAR g) modulator, that seems to have a positive effect on inhibiting adipogenesis in murine 3T3-L1 cells. We wanted to investigate further on its application on human cell models and try to understand its mechanism in inhibiting this phenomenon. The protocols were set up using the THP-1 cell line, which we noticed upon using a Lipid mixture cocktail (Composition: Non-animal fatty acids; 2 μg/ml arachidonic; 10 μg/ml linoleic acid; 10 μg/ml linolenic acid: 10 μg/ml myristic acid; 10 μg/ml oleic acid; 10 μg/ml palmitic acid; 10 μg/ml stearic acid; 0.22 mg/ml cholesterol from New Zealand sheep′s wool; 2.2 mg/ml Tween-80; 70 μg/ml tocopherol acetate), a decrease in pro-inflammatory cytokines IL-6 and IL-1b. We also noticed a doseIV dependent increase of FABP-4. Our findings regarding hADSCs, that PPARγ expression and lipid accumulation was restored upon the presence of lipid mixture in breast cancer hADSCs that were derived from breast tissue. Secondly, GMG-43AC in both concentrations (0.5mM and 2mM) inhibited lipid accumulation and showed a significant decrease in the expression of adipocyte-specific genes, such as PPARγ and FABP-4 even after the full differentiation of hADSCs that were derived from lipoaspirates. This suggests that dietary compounds are important factors in adipose differentiation and diet has a big influence in the progression and prevention in many metabolic diseases, such as obesity and cancer.
Hafner, Anne-Laure. "Étude des progéniteurs adipeux dérivés des cellules souches pluripotentes induites humaines." Thesis, Nice, 2015. http://www.theses.fr/2015NICE4062.
Full textIn mammals, two types of adipose tissue coexist: the white (WAT) wich is involved in energy storage and the brown (BAT) which is specialized in energy expenditure. Beige adipocytes have recently been described as brown –like adipocytes and represent a third type of adipocytes that are recruited in WAT. The molecular mechanisms involved in the generation of these different types of adipocytes remains unknow in humans, mainly because of the lack of appropriate in vitro cellular models. The human induced Pluripotent Stem (hips) cells are a good model to study the earliest steps of human adipogenesis. We have shown that the generation of white and brown adipocytes progenitors (AP) is regulated by acid retinoic signaling pathway during hips cells differentiation. Functional experiments indicated that the transcription factor Pax3 is a molecular mediator of the brown phenotype. During this study, we could see that AP derived from hips cells display a low adipogenic capacity as compared to progenitors derived from adult adipose tissue. We show in this work that treatment with TGFβ pathway inhibitor SB431542 together with ascorbic acid, hydrocortisone and EGF promoted differentiation of non- genetically modified hiPSCs-BAPs at a high rate. During preliminary results, we have analyzed the role of the transcription factor Hoxc8 on PA differentiation. The surexpression of this factor lead to distinct answers on the phenotype and differentiation between hiPSCs-AP and adult-derived AP
Yarmo, Michelle Nada. "The anti-adipogenic effect of macrophage-conditioned medium on on 3T3-L1 and human adipogenesis." Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28322.
Full textCarvalho, Polliane Morais de 1981. "Evaluation of masticatory, salivary and anthropometric function, presence of volatile sulphur compounds in young adults and changes in salivary gland pos adipogenesis induction in animal model = Avaliação da função mastigatória, salivar, antropométrica, presença de compostos sulfurados voláteis em adultos jovens e alterações em glândula salivar após indução de adipogênese em modelo animal." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/287939.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: Este estudo investigou a função mastigatória, o paladar, a presença de compostos sulfurados voláteis (CSV) e a bioquímica salivar em sujeitos saudáveis (18-33 anos), e a indução de adipócitos em glândula salivar e suas implicações em modelo animal. Três estudos foram conduzidos, apresentados na forma de capítulos. Capítulo 1: Investigou a performance e habilidade mastigatória, paladar e a possível relação com gênero, índice de massa corporal (IMC), circunferência de cintura (CC) e fluxo salivar estimulado (Stim) e não estimulado (Unst). Foram avaliados 171 indivíduos, (125? 46?). A performance mastigatória foi determinada pela capacidade de fragmentação do Optocal plus e habilidade mastigatória com o uso da escala visual analógica (EVA). O paladar foi verificado pela percepção dos quatro sabores primários. A estatística descritiva, teste de normalidade, correlação, comparação e modelos de regressão foram utilizados, sendo as variáveis dependentes a função mastigatória e o paladar e as independentes: gênero, idade, variáveis antropométricas e fluxo salivar. Na análise de regressão linear múltipla, as variáveis independentes não predisseram o modelo para performance mastigatória. Com a habilidade mastigatória (HM) o modelo explicou 14% da variabilidade e para o paladar 5%. Os resultados indicaram que a performance não foi relacionada com parâmetros antropométricos e salivares em indivíduos jovens saudáveis. A habilidade foi relacionada com IMC, CC e gênero. O paladar foi fracamente relacionado ao IMC e CC. Capítulo 2: Verificou a bioquímica salivar e presença de CSV e a possível interferência do IMC, fluxo e pH stim/unst. Para a verificação dos CSV foram avaliados 71 voluntários (57? 14 ?), utilizando o aparelho Oral chromeTM. Foram determinadas as concentrações de proteína total, cálcio, fosfato, amilase e ureia em 171 voluntários (125? 46 ?), em saliva stim/unst. A bioquímica salivar foi semelhante em relação à antropometria. No entanto, as respectivas concentrações diferiram significativamente entre saliva Stim/Unst, com exceção da amilase. Os indivíduos apresentaram quantidades semelhantes de CSV em relação ao IMC. Em indivíduos com valores críticos de metilmercaptana (CH3SH) observou-se correlação significativa (r=0.51) com o pH (unst). Capítulo 3: Investigou o eventual aparecimento de adipócitos nas glândulas salivares em modelo animal (camundongos). A adipogênese foi realizada com dieta rica em gordura, e ainda via receptor de proliferadores de peroxissoma gamma (PPAR gama) com rosiglitazona. Western blot, histoquímica e imunoistoquímica foram utilizadas. Análise de microarray foi realizada para verificar o efeito da dieta. Anticorpos: fosfo-4E BP1 e tirosina hidroxilase marcaram a atividade de mTOR e nervos, respectivamente. O microarray mostrou um número significativo de alterações genéticas. Em relação à dieta observou-se baixa ou nenhuma expressão de fosfo 4E-BP1 e aumento na atividade de tirosina hidroxilase. Em camundongos tratados com rosiglitazona verificou-se ativação de mTOR e tirosina hidroxilase. Conclusão: Pelos resultados dos três capítulos concluiu-se que em indivíduos jovens e saudáveis a função mastigatória e paladar não foram influenciados pelo padrão salivar e foram fracamente relacionados ao antropométrico. A bioquímica salivar e presença de CSV foi semelhante em relação à antropometria. Observaram-se mudanças relacionadas à atividade do sistema nervoso em glândula salivar de camundongos devido à dieta rica em gordura ou ativação de PPAR gamma
Abstract: This study investigated masticatory function, the presence of volatile sulfur compounds (VSC) and salivary biochemistry in healthy subjects (18-33 years), and also the induction of adipocytes in the salivary gland and its implication in an animal model. Three studies were conducted, presented as chapters. Chapter 1: Investigated the performance and chewing ability, taste, and the possible relationship to gender, body mass index (BMI), waist circumference (WC) and stimulated salivary flow (Stim) and unstimulated (Unst). 171 individuals (125? 46?) were evaluated. Masticatory performance was determined by the ability of fragmentation Optocal plus and chewing ability with the use of visual analogue scale (VAS). Taste was verified by the perception of the four primary flavors. Descriptive statistics, normality tests, correlation, comparison and multiple linear regression models were used. In multiple linear regression performance was not predict by the independent variables in the model. With chewing ability the model explained 14% of variability and 5% for the taste. The results indicated that masticatory performance was not related to anthropometric parameters and saliva in healthy young subjects. The ability was related to BMI, WC and Gender. Taste was weakly related to BMI and WC. Chapter 2: Verify the salivary biochemistry and presence of VSC and the possible influence of BMI, flow and pH (Stim)/(Unst). For the verification of VSC 71 volunteers (14 57? ?) were assessed using the Oral chromeTM device. The concentrations of: total protein, calcium, phosphate, urea and amylase were investigated in 171 volunteers (46 125? ?) in saliva (Stim) / (Unst). Biochemical salivary were similar in respect of anthropometry. However, the concentrations differed significantly between saliva (Stim)/(Unst), with the exception of amylase. The sample presented similar amounts of CSV in relation to BMI. In individuals with critical values of methylmercaptan (CH3SH) we observed a significant correlation (r = 0:51) with pH Unst. The results indicate that in healthy young subjects salivary biochemistry and VSC exhibit similar behaviour in relation to BMI. The (CH3SH) when greater than the normal limit concentration was correlated to pH Unst. Chapter 3: We investigated the possible appearance of adipocytes in the salivary glands in animal model (mice). Adipogenesis was performed with high-fat diet, and also via peroxisome proliferator-gamma (PPAR gamma) with rosiglitazone . Western blot, histochemistry and immunohistochemistry were used. Microarray analysis was performed to assess the effect of diet. Antibodies: phospho-4E-BP1 and tyrosine hydroxylase marked mTOR and nerves activity, respectively. The microarray showed a large number of genetic changes. Regarding diet was observed low or no expression of phospho-4E-BP1 and an increase in tyrosine hydroxylase activity. In mice treated with rosiglitazone there was activation of mTOR and tyrosine hydroxylase. The results suggest that there are changes in salivary gland innervation before stimuli for adipogenesis. Conclusion: We concluded that in healthy young individuals masticatory function was not influenced by the salivary pattern and was weakly related to anthropometric. Salivary Biochemical and presence of CSV was similar in relation to anthropometry. There are alterations in the activity of the nervous system in the salivary glands of mice due high fat diet or activation of PPAR gamma
Doutorado
Anatomia
Doutora em Biologia Buco-Dental
Guo, Heng. "Glucocorticoid induced leucine zipper is required for adipogenesis and is a target for the anti-adipogenic activities of oncostatin m." Thesis, Boston University, 2013. https://hdl.handle.net/2144/11007.
Full textFat cell development is a dynamic cellular transition process in which committed preadipocytes convert to adipocytes. The initial goal of my research was to identify repressed gene programs in adipogenesis, and to test the hypothesis that these repressed gene programs suppress adipogenesis. Using microarray analyses of 3T3-L1 cells, we found that the expression of gene programs, most notably cytokines/chemokines, correlated inversely with the differentiation state. We analyzed the effect of different components of the adipogenic hormonal cocktail in 3T3-L1 preadipocytes. It was found that dexamethasone (Dex) acting through the glucocorticoid receptor (GR) was a major suppressor of the cytokine expression. In our efforts to characterize roles for glucocorticoid signaling in adipogenesis, we found that glucocorticoid-induced leucine zipper (GILZ), a target of GR, was required for adipogenesis in both 3T3-L1 preadipocytes and C3H10T1/2 mesenchymal stem cells (MSCs). We also showed that GILZ was required for bone morphogenic protein 4 (BMP4)-induced white adipocyte differentiation and BMP7-induced brown differentiation in C3H10T1/2 MSCs. In a gain-of-function study, GILZ overexpression (OE) had minimal effects on normal adipogenesis in 3T3-L1 preadipocytes, but increased adipogenic gene expression in C3H10T1/2 MSCs. Oncostatin M (OSM) is an anti-adipogenic cytokine. When exploring mechanisms governing the anti-adipogenic activity of OSM and testing the hypothesis that GILZ is a target for OSM, we found that while OSM inhibited the expression of endogenous GILZ, ectopically expressed GILZ overrode OSM's effect in suppressing adipocyte development, suggesting GILZ is a target of the anti-adipogenic activity of OSM. During our studies to find signaling pathways regulating the interplay between GILZ and OSM, we found that OSM induced both ERK and STAT5 phosphorylation, but only inhibition of ERK activity by U0126 partially abolished OSM's inhibition on GILZ expression. Taken together, we identified a cytokine/chemokine program that is downregulated in adipogenesis. Dex-GR-GILZ signaling pathway played an essential role in suppressing the cytokine program and is required for adipogenesis. OSM inhibited adipogenesis by suppressing expression of GILZ, which is mediated by the activation of ERK phosphorylation. These findings highlighted the critical role of GILZ m adipogenesis and can contribute to combating metabolic disorders such as obesity.
SILVESTRINI, ANDREA. "Anti-inflammatory and anti-adipogenic activity of olive leaf extract and its bioactive compounds." Doctoral thesis, Università Politecnica delle Marche, 2022. https://hdl.handle.net/11566/299341.
Full textOlive leaves are a waste material from the olive oil industry, one of the staple foods of the Mediterranean diet. In recent years, scientific research has focused on the possibility of reusing this material because of its wealth of bioactive compounds that can be potentially applied in the biomedical field. the aim of this study was to characterise and evaluate the anti-inflammatory and anti-adipogenic effect of an aqueous olive leaf extract (OLE). The extract, produced in our laboratory from dried leaves harvested from herbicide- and pesticide-free cultivations, was characterised for its phenolic compound content (569.5±22.142 µg/mL in terms of Total Phenol content) and then tested on in vitro cellular models of acute and chronic inflammation and adipogenic differentiation. In particular, the acute effect was analysed in primary cultures of human umbilical vein endothelial cells (HUVEC) and in a human monocytic line (THP1) treated with LPS. The results obtained show a reduction in both the cytokine storm and the expression of adhesion molecules, whose function in the inflammatory process is to promote further recruitment of inflammatory cells into the circulation. The same HUVECs, but in replicative senescence, have been studied as a model of chronic inflammation, 'inflammaging' which in turn is associated with the onset of age- related diseases. Again, the extract inhibits the production of cytokines that characterise the SASP (Senescence associated secretory phenotype). Finally, given that accumulation of bone marrow fat is a common feature of several age-related diseases, and that adoptive tissue contributes to the body's systemic inflammatory state, we assessed whether OLE could inhibit the differentiation of human bone marrow stromal cells (MSCs) in an adipogenic direction. Again, we obtained promising results. In a second phase, we also analysed the effects of several active compounds contained in OLE and purified in the laboratory directed by Prof Antonio Procopio of the Magna Graecia University, one of the world's leading experts on olive tree active compounds. Two of these, oleacein and oleuropein-aglycone, have effects similar to those of the total extract, although the latter is always more effective than the single active compound. The research will continue by testing the effects of these same active ingredients microencapsulated in particles of biocompatible and mucoadhesive materials (chitosan or hyaluronic acid) that can be administered by areosol to subjects with Covid in order to reduce the cytokine storm as described in the FISR2020 project funded by the MUR, responsible Prof. ssa Rippo, and in which I am a participant. This study represents a first step towards the possibility of considering the use of OLE and its compounds as possible adjuvants in therapies for various acute and chronic diseases. The positive results obtained in models of inflammation, senescence and bone marrow fat accumulation are encouraging and stimulating for further research, confirming and expanding the possible practical implications of using natural products to support the treatment of different pathological conditions.
Arçari, Demétrius Paiva 1978. "Avaliação da erva-mate (Ilex paraguariensis) na adipogênese e sinalização da insulina." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317039.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: A obesidade e considerada um problema de saúde publica, principalmente pelo fato desta estar associada com diversas patologias como a resistência a insulina (RI). Atualmente, diversas estratégias são utilizadas visando a redução de peso corporal, dentre estas se destaca o uso de produtos de origem vegetal, incluindo a Ilex paraguariensis, cujo nome comum e erva-mate. Muitos trabalhos mostram que os compostos detectados na erva-mate possuem diferentes funções biológicas, tais como: ação antioxidante, antiinflamatória, imunomodulatoria, anticancerígena, modificação do metabolismo de colesterol, entre outros. Muito embora diversos estudos destaquem as funções biológicas da erva-mate, pouco se sabe sobre sua capacidade de modulação na expressão de genes relacionados a obesidade, e seu efeito na via de sinalização da insulina. Deste modo o presente estudo teve como objetivo avaliar a ação do extrato aquoso de erva-mate tostado no processo de adipogênese e sua ação nos mecanismos de sinalização da insulina. Os dados do presente trabalho mostram que a erva-mate na concentração de 1,0g/kg em animais submetidos a dieta hiperlipídica aumentou a expressão de diferentes genes responsáveis pela ativação da AKT, reduziu a translocação nuclear de NF-kB e FOXO1, reduziu a expressão PEPCK e G6Pase ligados ao processo de gliconeogênese no tecido hepático. Os efeitos da erva-mate na sinalização da insulina foram ratificados, por analise protéica de IRS-1, IRS-2 e AKT, redução na resistência a insulina observada pelo teste do KITT e redução da glicemia basal. O presente trabalho demonstra ainda em cultura celular de 3T3-L1 que a erva-mate e alguns de seus principais compostos bioativos (ácido clorogênico, rutina e quercetina), possuem ação mais expressiva na etapa de diferenciação do adipócito e atuam modulando distintos genes relacionados ao processo de diferenciação do adipócitos. O trabalho ainda sugere que a erva-mate possa atuar in vitro e ex vivo de maneira mais expressiva na redução da adipogênese através da via WNT, visto pelo aumento da expressão de diferentes genes relacionados com essa via. O resultado final da ativação desta via e a repressão significativa de PPARy2 e C/EBP'alfa', principais fatores de transcrição necessários para que ocorra a etapa final do processo de diferenciação dos adipócitos, contribuindo assim para elucidar a redução do peso corpóreo e da gordura epididimal observada nos animais submetidos a dieta hiperlipídica tratados com erva-mate durante 60 dias, que por sua vez reduz a produção de citocinas, em especial o TNF-'alfa', contribuindo parcialmente para a melhora do quadro de sinalização a insulina observado apos intervenção
Abstract: Obesity is a problem of public health, mainly because it is associated with many conditions such especially insulin resistance (IR). Currently, several strategies have been used in order to reduce the total body weight, among these there is a growing evidence supporting the use of products of plant origin, including the Ilex paraguariensis, whose common name is yerba mate. Various studies have shown that the compounds found in yerba mate has several biological functions, such as antioxidant, anti-inflammatory, immunomodulatory, anticancer, modification of cholesterol metabolism and others. Although several studies highlight the biological functions of yerba mate, there are lack of evidence providing their ability to modulate expression of genes related to obesity and its effect on the insulin signaling pathway. Thus, the aim of this study was to evaluate the effects of yerba mate in gene expression that regulate adipogenesis and insulin signaling pathway. Our data showed yerba mate (1,0 g/kg) in animals subjected to high fat diet, increased different gene expression responsible for the activation of the AKT, reduction of FOXO1 and NF-kB nuclear translocation, reduction gene expression of PEPCK and G6Pase involved in gluconeogenesis process in liver. The effects of yerba mate in insulin signaling was confirmed by IRS-1, IRS-2 and AKT protein analysis, reduction in insulin test tolerance by KITT and reduction in glucose. Our data also showed in 3T3-L1 cell culture that yerba mate and some of its major bioactive compounds (chlorogenic acid, rutin and quercetin), act in an early stage of adipocyte differentiation and modulate different gene expression that regulate adipogenesis. Additionally, yerba mate can act in vitro and ex vivo in WNT pathway, seen by the increased expression of different genes in this pathway resulting in a significant repression of C/EBP'alfa' and PPARy2, the most important transcription factors essencial for the occurrence of adipocyte differentiation. This findings collaborate to elucidate the reduction of body weight and epididymal fat observed in animals subjected to high fat diet treated with yerba mate for 60 days, which reduces the production of cytokines, particularly TNF-'alfa', contributing partially to the improvement in insulin signaling observed after intervention
Doutorado
Genetica Animal e Evolução
Doutor em Genetica e Biologia Molecular
Pastel, Emilie. "Rôles des aldose réductases dans l'homéostasie des tissus adipeux blancs humains et murins." Thesis, Clermont-Ferrand 2, 2014. http://www.theses.fr/2014CLF22492/document.
Full textAldose reductases are NADPH-dependent oxydoreductases described for their involvement in cellular detoxification and glucose reduction. The discovery of Akr1b7 expression in murine adipose tissue together with the prostaglandin F2α Synthase (PGFS) activity of some isoforms suggest unreleased biological roles for these enzymes. Prostaglandin F2α (PGF2α) inhibiting adipogenesis, this PGFS function highlights AKR1B potential involvement in white adipose tissue (WAT) physiology. This work aimed at characterising the expression of all AKR1B in both murine and human WAT and understanding their impact on adipose tissue homeostasis and especially on adipogenesis and lipolysis. We showed that all AKR1B were expressed in murine WAT. Akr1b3, Akr1b8 and Akr1b16 were both expressed in the stromal vascular fraction (containing immune cells, vascular cells, progenitors…) and in the adipose fraction. In contrast, Akr1b7 was not expressed in adipocytes. In vitro analyses indicated that, except for Akr1b16, murine AKR1B isoform expression increased early and transiently during adipogenesis. In human, AKR1B1 was expressed in human subcutaneous WAT from obese patients whereas AKR1B10 was hardly detectable (western blot, RT‑qPCR). In vitro, AKR1B1 expression increased throughout adipocyte differentiation unlike AKR1B10, which was preferentially expressed in undifferentiated cells. Using an AKR1B specific inhibitor, we demonstrated that AKR1B1 PGFS activity was a dampen to adipogenesis. We also showed that mechanisms regulating PGF2α action differed according to the species. In human cells, the expression of FP receptor was time-regulated whereas, in murine cells, PGFS expression and thus, PGF2α synthesis, limited PGF2α activity during adipogenesis. Akr1b7 knockout mice have decreased PGF2α intratissular levels associated with an expansion of adipose tissue resulting from an increase of adipogenesis and an adipocyte hypertrophia without any modification of lipogenic enzymes expression (Volat et al., 2012). These data, in agreement with PGF2α anti-adipogenic action, suggest an impact on lipolysis. We demonstrated that loss of Akr1b7 led to a decrease of WAT lipolytic activity. The use of murine (3T3‑L1) and human (hMADS) differentiated cells allowed us to show that the stimulation of lipolysis in response to FP activation was, in part, due to an increase of HSL phosphorylation (active form) and an increase of ATGL accumulation. The third part of this work consisted in characterizing the phenotype of transgenic mice overexpressing AKR1B1 in WAT (aP2‑AKR1B1 mice) in order to study the biological role of this human isoform
MacKay, Maria-Danielle L. "Characterization of Medullary and Human Mesenchymal Stem Cell-Derived Adipocytes." Case Western Reserve University School of Graduate Studies / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=case1232775772.
Full textBarlette, Adriana Gregory. "Avaliação química e biológica do extrato hidroetanólico de erva-mate (ilex paraguariensis a. st. hil.)." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2011. http://hdl.handle.net/10183/31784.
Full textIlex paraguariensis A. St.-Hil., known as maté, is a native tree from South América which leaves and twigs are used to prepare the traditional beverage “chimarrão”. It has a complex chemical composition that might have many potential applications in order to increase the use of maté and then, in consequence, increase the market demand of this raw material. Leaves from I. paraguariensis have xanthines, flavonoids, cafeoylquinic acid derivatives and triterpenoid saponins (ca. 10%). Herein, it was investigated, both chemically and biologically, the hydroethanolic extracts of leaves from I. paraguariensis, its fractions, and some reference substances, as the antioxidant activity, the adipogenesis (TG) and lipolytic activities in 3T3-L1 cell culture. So, it was prepared hydroethanolic extracts of fresh (EBV) and dried leaves (EBS) by maceration, which submitted to further fractionation furnished 8 fractions. Ursolic acid, chlorogenic acid and rutin were determined in these samples by liquid chromatography (HPLC) Also, phenolic constituents were determined using catequines and protein precipitation methods. The phenolic fraction from fresh leaves presented better antioxidant activity than the tested reference substances (rutin and chlorogenic acid), while ascorbic acid presented the best activity. The MTT assay showed that the tested extracts and fractions among 50 μg/mL and 1000 μg/mL did not present citotoxicity to 3T3-L1 cells. Among the tested fractions to adipogenesis, the aqueous residue fraction from fresh leaves (RAV) presented best inibition (24%) of TG at 100 μg/mL. Among tested reference substances, cafeic acid at 300 μg/mL, and rutin at 100 μg/mL presented the best results. In relation to the lipolytic activity, the RAV fraction presented best results at 50 e 75 μg/mL and, among the tested reference substances, galic acid presented best results at 500 e 1000 μg/mL. In order to understand the mechanim of action of these fractions and reference substances at the adipogenesis and lipolysis further studies will be performed specially those about the influence on the gene expression.
Muret, Kévin. "Annotation des ARN longs non-codants chez la poule et les espèces d’élevage : Focus sur les ARNlnc régulateurs du métabolisme des lipides." Thesis, Rennes, Agrocampus Ouest, 2018. http://www.theses.fr/2018NSARC139/document.
Full textGenome annotation is a major challenge in connecting genotypes with phenotypes. Identifying long noncoding RNAs (lncRNA) in genomes is part of this challenge; they are relatively low-expressed and have only been highlighted in 2012 thanks to the development of high throughput sequencing technologies. This research work has led to the identification of a large number of lncRNAs in livestock species, particularly in the chicken, in which no lncRNA had yet been described at the beginning of this thesis (2015). First, my aim was to identify these lncRNAs using liver and adipose tissue and to improve this catalogue by integrating other existing lncRNA public databases.Moreover, according to the literature, lncRNAs are involved in the regulation of any biological process, from gene expression to cell structure. One of the goals of our team is to understand the regulation of lipid metabolism in the chicken, I thus established the list of all lncRNAs known within the animal kingdom and involved in this metabolism or in adipogenesis, the process of storage and formation of adipose tissue. The conservation by synteny analyses revealed around twenty conserved lncRNAs in the chicken. From divergent abdominal fat weight chicken lines, I lastly identified new lncRNAs that potentially regulate this lipid metabolism
Volat, Fanny. "Rôle des aldose réductases dans la physiologie du tissu adipeux blanc : modèles génétiques murins perte et gain de fonction." Phd thesis, Université Blaise Pascal - Clermont-Ferrand II, 2011. http://tel.archives-ouvertes.fr/tel-00686589.
Full textSertié, Rogério Antonio Laurato. "Repercussões do destreinamento físico sobre o metabolismo e a celularidade do tecido adiposo branco periepididimal de ratos." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/42/42137/tde-10012011-164001/.
Full textAll adaptations acquired through physical training are reversible during inactivity. Significant reductions in maximal oxygen uptake (VO2Max) are observed within two-four weeks of detraining. Conversely, the consequences of detraining on adipose tissue are poorly known. The objective was to investigate the physical detraining effects on metabolism and cellularity of rat periepididymal adipose tissue. Methods and Results: Male Wistar rats, ageing 6 weeks, were divided in 3 groups: trained (T) for 12 weeks; detrained (D), (trained for 8 weeks and detrained for 4 weeks), and age-matched sedentary (S). Training consisted in treadmill running sessions (1h/day, 5d/week, 50 60% of the maximal capacity). The morphometric analysis of PE tissue disclosed significant differences between the groups. The adipocyte sectional area of group D was significantly bigger than T and S (3474 µm2 ± 68,8 µm2 vs 1945,7 µm2 ± 45,6 µm2 vs 2492,4 µm2 ± 49,08 µm2, respectively). Compared to T the cells of D animals showed 48% increased ability to perform: lipogenesis, either spontaneously or insulin stimulated and isoproterenol-stimulated lipolysis. Basal lipolysis did not change. A 15% reduction in apoptosis was observed in groups T and D in relation to S. Some gene expressions were changed in D vs S: adiponectin (3-fold up) and PPAR-gamma (2-fold up). PREF-1 gene was 3-fold higher in T vs S. These results strongly suggest that adipogenesis was stimulated in this group. Conclusions: Detraining causes significant increase in adipocyte size and lipogenic capacity. As PE fat cell apoptosis was reduced in D and T, these results suggest that adipose tissue changes following detraining can potentially be obesogenic.
Estrada, Marta Maria Vieira Matutino Falcão. "The role of gliptins on adipogenesis." Master's thesis, Faculdade de Ciências e Tecnologia, 2011. http://hdl.handle.net/10362/10732.
Full textThis work was performed at the Neuroendocrinology and Neurogenesis Group at the Center for Neurosciences and Cell Biology of the University of Coimbra, and financed by FCT (PTDC/SAUFCF/102415/2008) and FEDER
Boulet, Nathalie. "Rôles des Bone Morphogenetic Proteins dans la conversion adipocytaire et le développement du tissu adipeux humain." Thesis, Toulouse 3, 2015. http://www.theses.fr/2015TOU30187/document.
Full textAdipocytes (cells specialized in fat storage) arise from immature cells, called progenitor cells, during the process of adipogenesis. In human, the different stages of adipogenesis are not well defined as well as the signals involved in adipogenic modulation. The first part of my thesis work aimed to characterize the intermediate cell state between progenitor cell and mature adipocyte: the preadipocyte. The second part aimed to evaluate the role of bone morphogenetic proteins (BMPs) in human adipogenesis. In mice, BMP2 and BMP4 induce classical adipogenesis whereas BMP7 leads to the production of "brite" adipocytes with the capacity to use lipids to produce heat. We have shown that BMP2, 4 and 7 are produced in human fat depots and BMP7 is modulated by obesity. BMP2 and 4 induce classical adipogenesis and BMP7 only induces brite adipogenesis from human progenitor cells. These works improve our knowledge about the mechanisms involved in the expansion of fat depot and may allow the identification of new strategies to fight against the development of obesity-associated pathologies
Stillitano, Alexia. "miR-34a : a key regulator of adipogenesis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/206552.
Full textpublished_or_final_version
Medicine
Master
Master of Medical Sciences
Pereira, Angélica Costa Aranha Camacho 1976. "Efeito de um bloqueador do receptor PDGF na adipogênese de camundongos tratados com dieta hiperlipídica." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311204.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: A obesidade é hoje considerada um problema de saúde pública. Essa condição é caracterizada pelo aumento do peso corporal, mais especificamente do tecido adiposo branco. A adipogênese (diferenciação do pré-adipócito em adipócito) é um fenômeno complexo e não muito bem caracterizado. Recentes estudos mostraram que os préadipócitos estão localizadas nas paredes dos vasos que irrigam o tecido adiposo. Estas células estão presentes exclusivamente neste tecido e expressam alguns marcadores, dentre eles o PDGFRß. O PDGFRß é um receptor tirosina quinase cujo papel na migração, proliferação e diferenciação de diversos tipos celulares tem sido extensivamente estudado. O AG1296 (6,7- dimetoxi-2-fenil-quinoxalina) é um potente inibidor do receptor PDGF, pertencente à classe da quinoxalinas. Deste modo, levando-se em consideração o papel do receptor PDGF no crescimento e proliferação celulares e o fato de que as células PDGFR_ positivas provenientes do tecido adiposo possuem alto potencial adipogênico, neste estudo investigamos o efeito do AG1296 na adipogênese de camundongos submetidos à dieta hiperlipídica e à dieta padrão. Nós também investigamos se essa inibição afetaria a sensibilidade à insulina desses grupos estudados. Para tanto, camundongos Swiss machos com seis semanas de vida foram divididos em quatro grupos: o grupo Controle que recebeu dieta padrão, o grupo C+AG1296 que recebeu dieta padrão e tratamento com AG1296, o grupo DH que recebeu dieta hiperlipídica somente e o grupo DH+AG1296 que recebeu dieta hiperlipídica e tratamento com AG1296. Peso corpóreo e ingestão alimentar foram medidos diariamente durante o tratamento (7 ou 15 dias). Através de Western blot, foram quantificadas as principais proteínas pró-adipogênicas (SREBP-1c, C/EBP? e PPAR?) e a fosforilação das principais proteínas da via da insulina (IR, IRS1 e AKT). Nossos resultados indicaram que nos animais controle, após 15 dias de tratamento com AG1296, houve uma redução nas três frações de tecido adiposo, associada a uma redução em algumas das proteínas adipogênicas, além de uma melhora na sinalização insulínica em fígado e músculo e uma redução na glicemia de jejum. Além disso, nos animais submetidos à dieta hiperlipídica, após 7 dias de tratamento com AG1296, foi possível observar uma redução nas proteínas adipogênicas e uma redução na fração epididimal do tecido adiposo. Houve também uma melhora na sinalização insulínica e na tolerância à glicose. Com isso, podemos sugerir que a inibição do PDGFRß pode ter um papel importante na adipogênese e na sinalização insulínica e pode ser um alvo potencial para prevenção da obesidade e resistência à insulina
Abstract: Obesity can be defined as a disease in which body fat is excessively accumulated. Adipogenesis is a complex and not completely known phenomenon. Recent studies showed that adipocyte progenitor cells are exclusively found in adipose tissue and express some markers like PDGFRß (Platelet-derived growth factor ß). AG1296 (6,7-dimethoxy-2-phenyl-quinoxaline) is a potent and selective inhibitor of PDGF receptor kinase. In this context, the main objective of this work was to investigate if the inhibition of PDGF receptor through AG1296 would be able to affect white adipose tissue generation in high-fat-diet-fed and standard-chow-fed mice. We also investigated if this inhibition would have an effect on the insulin sensitivity in these studied groups. For this purpose, six-week-old male Swiss mice were divided into four groups and assigned to receive the following diet and/or treatment: the control group (C) received standard rodent diet, the second group (C + AG1296) received standard rodent diet plus AG1296 (50 mg/Kg/day by gavage), the third group (HFD) received high fat diet (55% calories from fat, 29% calories from carbohydrate and 16% from protein) and the fourth group (HFD+AG1296) received high fat diet plus AG1296. Body weight and food intake were measured during the treatment (7 and 15 days). After that, tissues (epididymal, retroperitoneal and mesenteric adipose tissue, liver and muscle) were extracted and processed. Through Western blot analysis, we were able to quantify the main proteins related to adipogenesis (SREBP-1c, C/EBP? e PPAR?) and the phosphorylation of the main proteins from insulin pathway (IR, IRS1 and Akt). Our results indicated that on control mice, after 15 days of treatment with AG1296, there was a reduction on adipose fat pad, associated with reduction in some adipogenic proteins, an increase in insulin signaling in liver and muscle and a reduction in fasting plasma glucose. Futhermore, on mice fed a high fat diet, after 7 days of treatment with AG1296, it was possible to observe a reduction on adipogenesis proteins and a reduction in epididymal fat pad. Also, there was an improvement in insulin signaling pathway and in glucose tolerance. In conclusion, our results suggest that PDGFRß inhibition might have an important role in adipogenesis and in insulin signaling and could be a potential target for preventing obesity and insulin resistance
Mestrado
Biologia Estrutural, Celular, Molecular e do Desenvolvimento
Mestre em Ciências
Sharafi, Parisa. "The Effect Of Mechanical Forces On Adipogenic Differentiation." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/12609224/index.pdf.
Full text0.2 g, 7.5 ±
0.2 g, and 14.6 ±
0.3 g.) continuously in differentiation medium for 21 days. The control discs were treated with differentiation medium without any compressive weight on top of them. After 21 days, total ribonucleic acids (RNA) have been isolated. Adipogenic differentiation was investigated via reverse transcription coupled quantitative polymerase chain reaction (PCR). The expression of peroxisome proliferators-activated receptors (PPAR-gamma), CCAAT-enhancer binding protein (C/EBP-Beta), leptin, adiponectin, adipophilin and human stearoyl-CoA desaturase (hSCD) have been assessed as adipogenic markers. Differentiation to adipocytes has been further investigated by histochemical Sudan IV staining and immunochemistry and compared to control group. Decrease in the expression of adipogenic factors, size and number of lipid droplets were observed for both MSCs and preadipocytes subjected to compression in agarose discs. The decreases were correlated with the level of mechanical stress. The highest depletion of gene expression was observed in leptin and C/EBP&
#61538
. From our results, it was shown for the first time that mechanical stress impaired the adipogenic differentiation of MSCs and preadipocytes in agarose discs. However, the differentiation pathways should be further investigated.
Chan, Cheuk-ying, and 陳倬瑩. "Effects of (-)-epigallocatechin gallate in 3T3-L1 adipogenesis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B44670679.
Full textHeath, Victoria J. "Inhibition of adipogenesis by the c-myc oncoprotein." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360306.
Full textCassidy, Fearon. "The role of Dlk1 in in vivo adipogenesis." Thesis, Queen Mary, University of London, 2018. http://qmro.qmul.ac.uk/xmlui/handle/123456789/46028.
Full textChristodoulides, Constantinos. "The role of Wnt signalling in human adipogenesis." Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613010.
Full textWu, Yu. "Identification and Functional Characterization of Adipogenesis-related Genes." University of Toledo Health Science Campus / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1229546422.
Full textAdams, Vanessa Lynn. "Adipogenesis in post-weanling pigs fed conjugated linoleic acid." Thesis, Texas A&M University, 2004. http://hdl.handle.net/1969.1/1091.
Full textAbood, Steven. "The Effects of Artemisia Derived Natural Products on Adipogenesis." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3383.
Full textTsuji, Wakako. "Adipogenesis induced by human adipose tissue-derived stem cells." Kyoto University, 2009. http://hdl.handle.net/2433/126447.
Full textXaymardan, Munira. "Structural differentiation in cultured endothelial clusters and adipogenic healing." Thesis, The University of Sydney, 2001. http://hdl.handle.net/2123/4715.
Full textCampos, Carolina Filardi de. "Gene expression and proteomic analysis underlying adipogenesis in livestock." Universidade Federal de Viçosa, 2016. http://www.locus.ufv.br/handle/123456789/9505.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico
Marmoreio ou gordura intramuscular (IMF) é um componente importante da produção animal, já que é um dos principais fatores para a qualidade de carne. IMF é alcançada pela adipogênese, processo de proliferação e diferenciação de pré-adipócitos, e pela lipogênese, com a assimilação subsequente de lipídeos. A compreensão dos eventos que ocorrem durante a diferenciação de pré-adipócitos tem avançado consideravelmente nos últimos anos e baseou-se principalmente no uso de cultura de tecidos. Os modelos animais podem ser utilizados não apenas para a melhor compreensão da deposição de gordura, mas também como modelos para maior compreensão sobre os mecanismos moleculares subjacentes a determinadas condições humanas. Além disso, a diferenciação em adipócitos tem muitas implicações para doenças em humanos. É sabido que esta diferenciação envolve uma cascata transcricional, de modo que o presente estudo proporciona conhecimento sobre genes e fatores de transcrição utilizando a técnica de RT-qPCR, envolvidos na diferenciação de pré-adipócitos em adipócitos maduros comparando duas raças divergentes de suínos. Os resultados mostram que alguns genes são diferencialmente expressos entre a linhagem comercial e a raça Piau. Além disso, um estudo de expressão gênica e um estudo proteômico são fornecidos revelando os efeitos da vitamina A sobre a adipogênese em bovinos, revelando efeito negativo sobre a adipogênese. Assim, destacamos a importância da expressão gênica e dos estudos proteômicos para aumentar a compreensão dos mecanismos moleculares envolvidos na adipogênese.
Marbling or intramuscular fat (IMF) is an important component of livestock production, as it is a major factor in the overall meat quality. IMF is achieved by adipogenesis, the process of proliferation and differentiation of preadipocytes, and lipogenesis, with the subsequent assimilation of lipid. The understanding of events that occur during preadipocyte differentiation has advanced considerably in the last few years and has relied mainly on the use of tissue culture models of adipogenesis. Animal models can be used not only for a better understanding of fat deposition in livestock, but also as models to an increased comprehension on molecular mechanisms behind human conditions. Furthermore, adipocyte differentiation has many implications for human disease conditions. It is well known that this differentiation involves a transcriptional cascade, so the present study provided knowledge about genes and transcription factors using RT-qPCR technique, involved in differentiation of preadipocytes into mature adipocytes comparing two divergent pig breeds. The results show us that some genes are differentially expressed between commercial line and Piau breed. Moreover a gene expression and a proteomic study are provided revealing the effects of vitamin A on adipogenesis in cattle, revealing the negative effect on adipogenesis. Thereby, we highlighted the importance of gene expression and proteomic studies to increase the understanding of molecular mechanisms underlying adipogenesis.
Hadadeh, Ola. "Rôle du système d'activation du plasminogène dans la différenciation des cellules souches embryonnaires de souris." Thesis, Aix-Marseille 2, 2011. http://www.theses.fr/2011AIX20718.
Full textRegulation of the extracellular matrix (ECM) plays an important functional biological role either in physiological or pathological conditions. The plasminogen activation (PA) system, comprising the uPA and tPA proteases and their inhibitor PAI-1, is one of the main suppliers of extracellular proteolytic activity contributing to tissue remodeling. Although its function in development is well documented, its precise role in mouse embryonic stem cell (ESC) differentiationin vitro is unknown. We found that uPA and tPA activities and PAI-1 protein are very low in undifferentiated ESCs and increase strongly during the differentiation, reaching a maximum in well differentiated cells. Adipocyte formation by ESCs is inhibited by amiloride treatment, a specific uPA inhibitor. Likewise, ESCs expressing ectopic PAI-1 under the control of an inducible expression system, display reduced adipogenic capacities after induction of the gene. Our results demonstrate that the control of ESC adipogenesis by the PA system correspond to different successive steps from undifferentiated to well differentiated ESCs. Furthermore, the adipogenic differentiation capacities of PAI-1-/- induced pluripotent stem cells (iPSCs) are augmented as compared to wt iPSCs. Similarly, skeletal myogenesis is decreased by uPA inhibition or PAI-1 overexpression during the terminal step of differentiation. However, interfering with uPA during days 0 to 3 of the differentiation process augments ESC myotube formation. Neither neurogenesis, cardiomyogenesis, endothelial cell nor smooth muscle formation are affected by amiloride or PAI-1 induction. Our results show that the PA system is capable to specifically modulate adipogenesis and skeletal myogenesis of ESCs by successive different molecular mechanisms
Moratal, Claudine. "Les progéniteurs fibro-adipogéniques des muscles squelettiques humains sains et dystrophiques : caractérisation et interactions avec les progéniteurs myogéniques et les macrophages." Thesis, Université Côte d'Azur (ComUE), 2016. http://www.theses.fr/2016AZUR4128/document.
Full textMuscle regeneration involves functional interactions between different types of mononuclear cells including myogenic progenitors (MPs) and macrophages. Following injury, damaged muscles are invaded by immune cells and MPs fuse to generate new myofibres. Transient fibrotic and adipocyte deposits are observed in regenerating muscles, which however persist in Duchenne muscular dystrophy (DMD) and during aging. We demonstrated that fibro-adipogenic progenitors (FAPs) expressing the PDGFRα surface marker would contribute to the development of non-myogenic deposits in healthy muscles. Indeed, these progenitors differentiate into functional white adipocytes that have the feature to be insulino-resistant, and give rise to myofibroblastes. Intramuscular fibrosis in DMD patients could be formed from both FAPs and MPs differentiation. In healthy muscles, FAPs stimulate myogenesis of MPs during regeneration, while myotubes and pro-inflammatory macrophages inhibit the adipogenesis and fibrogenesis of FAPs. Cellular interactions between FAPs and MPs are disrupted for DMD or aged progenitors. Interestingly, they are restored if aged or DMD FAPs are replaced by healthy and young MPs. Our results show that the human muscles contain fibro-adipogenic progenitors that play a crucial role in the control of muscle homeostasis by interacting with myogenic progenitors and macrophages
Joe, Aaron Wai Bun. "Identification, isolation and characterization of murine adult adipogenic progenitor cells." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/22316.
Full textAl-Jabir, M. J. M. H. "Regulation of adipogenesis and inflammation : role(s) of adipose microRNAs." Thesis, University College London (University of London), 2016. http://discovery.ucl.ac.uk/1473942/.
Full textTam, Joshua. "Adipogenesis and angiogenesis : roles in tissue engineering and glucose metabolism." Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/54590.
Full textCataloged from PDF version of thesis.
Includes bibliographical references (p. 118-127).
Adipose tissue serves two main functions in the body: (1) it is the body's primary energy depot; and (2) it also serves as an important endocrine organ, producing and secreting various enzymes, growth factors, cytokines, and hormones. Both of these functions require ample access to circulating blood. Many aspects of angiogenesis during adipose tissue expansion remain poorly understood. Adipocytes produce a large variety of molecules involved in angiogenesis, and obesity is associated with elevated circulating levels of Vascular Endothelial Growth Factor (VEGF). Our lab has previously shown that angiogenesis and adipogenesis are mutually dependent via a VEGF receptor 2 (VEGFR2)-mediated mechanism. Since then several other studies have reinforced a role for the VEGF-VEGFR system in energy metabolism. For example, genetically obese mice treated with anti-VEGF antibody had lower fat pad weights, but the VEGF receptor responsible for this observation is not known. There is also disagreement on the cell type(s) responsible for fat tissue's angiogenic capability, with some studies supporting a dominant role for adipocytes, while others attribute most of the angiogenic capacity to the adipose tissue stromal cells (ASC). This thesis project aimed to fill some of these gaps by examining the angiogenic capacity of adipose tissue relative to other tissues, the effects of VEGFR-1 and R-2 blockade in mouse models of adipogenesis and diet-induced obesity, the respective angiogenic capabilities of adipocytes and ASC, and the possibility of harnessing the angiogenic potential of adipose tissue for vascular tissue engineering.
(cont.) In addition, a physiologically-based mathematical model was developed to simulate the regulatory effects of the leptin pathway on murine energy homeostasis.
by Joshua Tam.
Ph.D.
Lee, Aishlin Elizabeth. "Selenium Treatment Promotes Adipogenesis in Chicken Embryonic Fibroblasts In Vitro." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1367416159.
Full textCawthorn, W. P. "Molecular mechanisms of anti-adipogenesis by tumour necrosis factor-alpha." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597378.
Full textAGUIARI, Paola. "High Glucose Induces Adipogenic Differentiation of Muscle-Derived Stem Cells." Doctoral thesis, Università degli studi di Ferrara, 2009. http://hdl.handle.net/11392/2388681.
Full textBatrakou, Dzmitry G. "Nuclear envelope transmembrane proteins in differentiation systems." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/9981.
Full textPeshdary, Vian. "Effect of Glucose on Human Adipogenesis and its Regulation by Macrophages." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/35051.
Full textAbaiian, Kayvan Jasper. "Regulation of aortic carboxypeptidase-like protein (ACLP) during 3T3-L1 adipogenesis." Thesis, University of Ottawa (Canada), 2001. http://hdl.handle.net/10393/9355.
Full textMuise, Aleixo Michael. "Adipocyte enhancer binding protein (AEBP1), a multifunctional protein involved in adipogenesis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24756.pdf.
Full textTam, Ka-shing, and 譚家承. "Effects of bitter melon extracts on adipogenesis of 3T3-L1 adipocytes." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42182037.
Full textPell, Vidal Núria. "CPEB4-Driven adipocyte reprogramming is required for adipogenesis and pathological inflammation." Doctoral thesis, Universitat de Barcelona, 2020. http://hdl.handle.net/10803/671750.
Full textYamada, Tomoya. "Studies on the expression of adipogenic transcription factors in beef cattle." Kyoto University, 2011. http://hdl.handle.net/2433/135406.
Full textNoguchi, Michio. "Genetic and pharmacological inhibition of Rho-associated kinase 2 enhances adipogenesis." Kyoto University, 2008. http://hdl.handle.net/2433/135793.
Full textTam, Ka-shing. "Effects of bitter melon extracts on adipogenesis of 3T3-L1 adipocytes." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42182037.
Full textGuth, Sinja Viktoria [Verfasser]. "Untersuchungen zur Differenzierung equiner, adipogener, mesenchymaler Stammzellen zu Tenozyten / Sinja Viktoria Guth." Gießen : Universitätsbibliothek, 2013. http://d-nb.info/1065251297/34.
Full textMoseti, Dorothy. "25 Hydroxycholesterol inhibits adipogenesis and expression of adipogenic transcripts in C3H10T1/2 mouse stem cells independent of hedgehog signalling mechanism." 2015. http://hdl.handle.net/1993/30577.
Full textOctober 2015
Deuschl, Jana Daniela. "Der Einfluss des Transkriptionsfaktors Runx2 auf osteogene und adipogene Differenzierungsmarker, insbesondere auf PPARγ." Doctoral thesis, 2013. http://hdl.handle.net/11858/00-1735-0000-0022-5DE8-D.
Full textIannantuono, Nicholas. "Régulation du facteur de transcription FOXK1 par O-GlcNAcylation : implications dans la différenciation adipocytaire." Thèse, 2015. http://hdl.handle.net/1866/13646.
Full textPost-translational modifications such as phosphorylation, O-GlcNAcylation and ubiquitination play critical roles in coordinating protein function and are therefore involved in diverse cellular processes. Of relevance here, ubiquitination may be removed by deubiquitinases such as the tumour suppressor BAP1, which represents the most mutated deubiquitinase gene in the human genome. Recent studies have revealed that important and dynamic post-translational modifications regulate several functions of the BAP1 complex. Indeed, BAP1 has been shown to form a multi-protein complex with several transcriptional regulators including the polycomb group protein OGT and the transcription factors FOXK1 and FOXK2. OGT is a unique enzyme that catalyzes the addition of an O-GlcNAc moiety to target proteins, which impacts protein function including enzymatic activity, protein-protein interactions and subcellular localization. This modification is also highly linked to cellular metabolism, as the donor substrate for the reaction, UDP-GlcNAc, is derived from the hexosamine biosynthesis pathway. Similarly, FOXK1 and FOXK2 have been shown to be implicated in metabolic processes such as myogenesis and autophagy. During our studies, we identified FOXK1 but not FOXK2 as a novel substrate of OGT. Further, we found that this OGlcNAcylation is modulated during the entry/exit of cell cycle. We also found that FOXK1 is critical for adipogenesis and that the interaction between FOXK1/BAP1 is compromised during nutrient starvation. Thus, our studies have revealed that OGT selectively modulates and regulates components of the BAP1 complex which may impact different cellular processes, notably chromatin remodelling and could help understanding how BAP1 acts as a tumor suppressor.
Labrecque, Benoît. "Identification de gènes impliqués dans le développement du tissu adipeux et caractérisation de PON3 et de son impact sur divers paramètres de production chez le porc." Thèse, 2008. http://hdl.handle.net/1866/6403.
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