Dissertations / Theses on the topic 'Actinobacteria'
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NERYS, Laís Ludmila de Albuquerque. "Avaliação das atividades antimicrobiana e anticâncer de metabólitos produzidos por Streptomyces sp UFPEDA 3407." Universidade Federal de Pernambuco, 2015. https://repositorio.ufpe.br/handle/123456789/18542.
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Actinobactérias são bactérias Gram-positivas que se destacam pela sua grande potencialidade de produzir diversos metabólitos secundários bioativos de interesse científico e industrial. Mais de 50% dos metabólitos microbianos bioativos descobertos são produzidos, tais como antimicrobianos e antitumorais, pelas actinobactérias principalmente pelo gênero Streptomyces.O câncer é considerado um importante problema de saúde pública em países desenvolvidos e em desenvolvimento, sendo a segunda causa de morte no Brasil e no mundo. Diante da diversidade de tecnologias e pesquisas aplicadas no campo das neoplasias, ainda não há uma terapia eficaz para o tratamento de todos os tipos de câncer e os quimioterápicos utilizados atualmente apresentam elevada toxicidade. Neste contexto, o presente projeto objetivou avaliar o potencial antimicrobiano e anticâncer dos extratos brutos produzidos pela por Streptomyces UFPEDA 3407. Após fermentação e extração dos metabólitos com diferentes solventes, foram realizados ensaios para avaliar a atividade antimicrobiana e citotóxica nas diferentes linhagens de células cancerígenas humanas (HL- 60, HT-29 e MCF- 7) e em eritrócitos murinos. Os resultados obtidos demonstraram que os extratos da biomassa extraídos com acetona, etanol e metanol apresentaram um bom espectro de ação contra bactérias Gram-positivas e leveduras. Nos ensaios de citotoxicidade, os mesmos extratos reduziram de maneira significativa a viabilidade das linhagens tumorais, mostrando resultados bastante promissores.
Actinomycetes are Gram-positive bacteria with high potential to produce various bioactive secondary metabolites of scientific and industrial interest. More than 50% of bioactive microbial metabolites discovered to date are produced by actinomycetes mainly by gender Streptomyces. Cancer is considered a major public health problem in developed and developing countries, and the second cause of death in Brazil and in the world. Given the diversity of technologies and applied research in the field of cancer, there is still no effective therapy for the treatment of all types of cancer and the chemotherapy drugs used today have high toxicity. In this context, this project aimed to evaluate the antimicrobial potential anticancer and crude extracts produced by Streptomyces actinobacteria UFPEDA 3407. After fermentation and extraction of metabolites with different solvents, tests were conducted to evaluate the antimicrobial and cytotoxic activity in different strains of human cancer cells (HL- 60, HT-29 and MCF-7) and murine erythrocytes. Partial results showed that the extracts of biomass extracted with acetone, ethanol and methanol had a good spectrum of action against Gram-positive bacteria and yeasts. In the cytotoxicity assays, the same extracts significantly reduced the viability of tumor cell lines, showing promising results.
Watson, Daniel John. "Screening of actinobacteria for novel antimalarial compounds." Doctoral thesis, Faculty of Health Sciences, 2021. http://hdl.handle.net/11427/33080.
Full textBERVANAKIS, GEORGE, and gberva@hotmail com. "DETECTION AND EXPRESSION OF BIOSYNTHETIC GENES IN ACTINOBACTERIA." Flinders University. School of Medicine, 2009. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20090531.033038.
Full textWang, Xiaoling. "Natural product discovery and biosynthesis from soil actinobacteria." Thesis, University of Aberdeen, 2013. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203796.
Full textGarcia, Carlos Eduardo. "Isolamento e identificação de actinobacterias em solos de terra preta antropogenica (TPA) da Amazonia Central por ARDRA e sequenciamento do gene 16S rRNA." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256616.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Doutorado
Doutor em Ciência de Alimentos
Conn, Vanessa Michelle, and vanessa conn@acpfg com au. "Molecular Interactions of Endophytic Actinobacteria in Wheat and Arabidopsis." Flinders University. School of Medicine, 2006. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20060320.171412.
Full textSchäfer, Jenny [Verfasser]. "Untersuchungen zur Diversität von Actinobacteria in Innenräumen / Jenny Schäfer." Gießen : Universitätsbibliothek, 2012. http://d-nb.info/1063954231/34.
Full textSantos, Luísa Ferreira dos. "Metabolic engineering of actinobacteria for the production of flavours." Thesis, université Paris-Saclay, 2022. http://www.theses.fr/2022UPASL034.
Full textThis thesis was carried out in collaboration with ENNOLYS, a French biotechnology company specialised in the production of biomass, enzymes, and natural aromatic molecules. In this context, the main objective of this thesis project is the metabolic engineering of actinobacteria, including those of the genus Amycolatopsis, for the development and improvement of bioproduction processes for natural aromatic molecules, including vanillin, the most used flavouring agent in the world. To this end, we sequenced the genome of the industrial strain Amycolatopsis ZYL926 in order to better understand the potential of this bacterium in the production of specialised metabolites and in the biosynthesis of vanillin. Furthermore, we were able to identify genes involved in the degradation of vanillin (or of its biosynthetic intermediates) by sequence comparison analysis. Secondly, we have identified genetic tools (including vectors and promotors) for the stable insertion and efficient expression of heterologous genes in Amycolatopsis. In addition, we have developed tools for marker-free deletion of genes or large genomic regions in these bacteria. These genetic tools are versatile and could be used in many species of the genus Amycolatopsis. Finally, in order to implement a vanillin biosynthetic pathway from a low-cost substrate, we selected and studied a few candidate enzymes for each new biosynthetic step. These studies enabled us to identify among the candidate enzymes those which are active in Amycolatopsis under the bioconversion conditions used industrially. In addition, we were able to study a limiting step and suggest ways to improve the efficiency of the studied biosynthetic pathway
Mevaere, Jimmy. "Lasso peptides from Actinobacteria - Chemical diversity and ecological role." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066617/document.
Full textLasso peptides are ribosomally synthesized and post-translationally modified peptides produced by bacteria, characterized by a remarkable mechanically-interlocked structure. The lasso topology, reminiscent to a rotaxane, consists in an N-terminal macrolactam ring threaded by a C-terminal tail. This compact and stable structure is stabilized by steric entrapping of the tail in the ring, through bulky amino acid(s) and/or disulphide bonds. Lasso peptides produced by Actinobacteria display the greatest chemical diversity and a range of biological activities (antibacterial, anti-HIV, receptor antagonist…), therefore are of high pharmaceutical interest. Genome mining revealed that Actinobacteria have enormous potential to biosynthesize novel lasso peptides, e.g. harbouring new post-translational modifications. However, the expression of these peptides is generally controlled by complex regulatory systems, making their production under laboratory conditions difficult. Understanding the ecological role and regulation mechanisms of lasso peptides would help to improve production and better exploit the biotechnological potential of these molecules. The first part of my work deals with the identification of new lasso peptides from Actinobacteria, using heterologous expression in Streptomyces hosts. The second part of my work deals with the regulation mechanism and ecological role of lasso peptides using sviceucin, a lasso peptide produced by Streptomyces sviceus, as the model for study
Silva, ValÃria Maria AraÃjo. "Facilitation can increase actinobacteria adaptation capacity and rhizobia "in vitro"." Universidade Federal do CearÃ, 2016. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=17158.
Full textThe natural environment is marked by an intricate network of biotic interactions that shape the structure of ecological communities. The presence of positive ecological interactions between microbial populations in soils semiarid regions, has great importance in structuring the local soil microbiota. In this work, actinomycetes strains of rhizobia and coming from rhizosphere of Park National Ubajara-CE, were evaluated for the ability to grow through cooperative metabolic mechanisms. Of the 27 evaluated actinomycetes, 22 showed compatibility with rhizobia. The strains UB-05, UB-07, UB-08, UB-11 and UB-21 stood out in facilitating tests for amylase and cellulase. The metabolic activity of actinomycetes helped the development of rhizobia strains
O ambiente natural à marcado por uma intrincada rede de interaÃÃes biÃticas que moldam a estrutura das comunidades ecolÃgicas. A presenÃa de interaÃÃes ecolÃgicas positivas entre populaÃÃes microbianas em solos de regiÃes semiÃridas, possui grande relevÃncia na estruturaÃÃo da microbiota do solo local. Neste trabalho, cepas de actinobactÃrias e rizÃbios oriundas de solo rizosfÃrico do Parque Nacional de Ubajara-CE, foram avaliadas quanto à capacidade de crescerem atravÃs de mecanismos metabÃlicos cooperativos. Das 27 actinobactÃrias avaliadas, 22 apresentaram compatibilidade com rizÃbios. As cepas UB-05, UB-07, UB-08, UB-11 e UB-21, destacaram-se nos ensaios de facilitaÃÃo para amilase e celulase. A atividade metabÃlica de actinobactÃrias auxiliou o desenvolvimento das cepas de rizÃbios.
Sanyika, Walter Tendai. "Comparison of actinobacterial diversity in Marion Island terrestrial habitats." Thesis, University of the Western Cape, 2008. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_2619_1263423621.
Full textMoody, Suzy Clare. "Exploring the roles of novel secondary metabolites in Streptomycetes." Thesis, Swansea University, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678483.
Full textMokhtar, Noor Azlin. "Investigations of triacyglyceride metabolism amongst actinomycete isolates from Peninsular Malaysia." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609654.
Full textSpadari, Cristina de Castro. "Bioprospecção de uma substância antifúngica potencialmente nova produzida por actinomicetos isolados no RS." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/79634.
Full textThe dermatophytes fungi and Candida species are some of the microorganisms responsible for mycoses in humans. The treatment of these diseases eventually becomes difficult by the low number of antifungal agents in the market and by the emergence of resistance to the drugs available today. The actinobacteria are known by producing different secondary metabolites. This study aimed to evaluate the antifungal activity of secondary metabolites produced by actinomycetes isolates against fungal pathogens of clinical origin. For the selection of actinomycetes isolates with the best potential inhibition the assay of double layer was performed on starch casein agar (SCA). The isolates that showed activity were submitted to an optimization of the active compound production in submerged culture system. In order to do so, different culture media, temperatures and pH ranges were assessed. The antifungal activity was evaluated every 24 hours for eight days and activity was observed by well diffusion assay, where the antibiosis index was calculated. None of the dermatophyte isolates were inhibited in the double layer assay. Isolates 1S, R18(6) and 6(2) of the actinobacteria demonstrated activity against all the tested Candida species. Isolate R18(6) showed the highest activity in liquid culture and the best growing conditions for producing the antifungal compound was media starch casein broth , without pH control, temperature of 30°C with 72h of cell growth. Assays were performed as the minimum inhibitory concentration, thermal stability test and the effect of different enzymes on antifungal activity of the crude extract. The crude extract was subjected to standardized thin layer chromatography (TLC) with different solvents and an autobiography assay was conducted to verify band with antifungal activity. The active compound was observed at an Rf of 0.35 when the solvent mixture of butanol/ acetic acid/ water was used. After identifying the band with antifungal activity CCD coloring tests were performed with ferric chloride, anisaldehyde and ninhydrin for partial identification of the active compound; it has been observed that the compound does not have hydroxyl groups attached to the aromatic ring, it has no free amino group and possibly some part of the molecule has a linked nitrogen heterocyclic ring. Also, we performed a morphological characterization of the isolate R18 (6) through microcultive and scanning electron microscopy and structures have been observed characteristics of the genus Streptomyces.
Souza, Alessandra Zanin Zambom de 1987. "A suplementação via oral com L-glutamina altera a composição da microbiota intestinal de indivíduos sobrepesos e obesos." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/244481.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Aplicadas
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Resumo: Introdução: Inúmeros fatores contribuem para o aumento da obesidade em todo o mundo. Recentemente, a microbiota intestinal ganhou destaque devido ao seu poder de predispor ou inibir o ganho de peso. Alguns nutrientes são capazes de alterar a composição da microbiota intestinal, o que pode trazer efeitos benéficos ou maléficos, como a obesidade. O aminoácido L-glutamina, além de suas inúmeras funções orgânicas e imunológicas, é conhecido por desempenhar importante papel no trofismo intestinal. O objetivo do presente estudo foi investigar alterações na composição da microbiota intestinal de indivíduos com sobrepeso ou obesidade após suplementação oral com L-glutamina. Métodos: Voluntários com sobrepeso ou obesidade foram selecionados para ingerir 30g de L-glutamina (GLN) por via oral ao dia, por um período de quatorze dias. O grupo controle recebeu L-alanina (ALA) no mesmo tempo e proporção. Amostras de sangue e fezes foram coletadas para análises. Para classificação taxonômica das bactérias intestinais, foi realizado sequenciamento do gene 16S RNA ribossomal. Análises de bioinformática foram conduzidas com base no banco de dados RDP (Ribosomal Database Project). Para análise dos dados, estratégias estatísticas variadas foram utilizadas. Resultados: Após quatorze dias de suplementação, os participantes do grupo GLN exibiram diferenças significativas nos filos Actinobacteria e Firmicutes e nos gêneros Dialister, Dorea, Pseudobutyrivibrio e Veillonella, comparados com o grupo ALA. A razão F / B (Firmicutes / Bacteroidetes), um bom biomarcador para a obesidade, reduziu de 0,85 para 0,57 no grupo GLN e ao contrário, aumentou de 0,91 para 1,12 no grupo ALA. Conclusão: A suplementação oral do aminoácido L-glutamina, em humanos com sobrepeso e obesidade, por um período de quatorze dias, promove alterações na composição da microbiota intestinal similares às promovidas pela perda de peso
Abstract: Introduction: Several factors contribute to the increase of obesity worldwide. Recently, the gut microbiota gained prominence due to its power to predispose or inhibit weight gain. Some nutrients are able to change the composition of the gut microbiota, what can bring beneficial or harmful effects, such as obesity. The amino acid L-glutamine, in addition to its numerous organic and immune functions, is known to play an important role in intestinal tropism. The aim of this study was to investigate changes in the composition of the gut microbiota of overweight or obese adults after oral supplementation with L-glutamine. Methods: Overweight or obese subjects were selected to orally ingest 30g of L-glutamine (GLN) daily for a period of fourteen days. The control group received L-alanine (ALA) in the same period and proportion. Blood and feces were collected for analysis. The 16S rRNA gene sequence was performed for taxonomic classification of intestinal bacteria. Bioinformatics analysis was conducted based on RDP (Ribosomal Database Project). For data analysis, varied statistical strategies were used. Results: After fourteen days of supplementation, participants in the GLN group showed significant differences in the Firmicutes and Actinobacteria phyla and Dialister, Dorea, Pseudobutyrivibrio and Veillonella genera, compared with the ALA group. The F / B (Firmicutes / Bacteroidetes) ratio, a good biomarker for obesity, decreased from 0.85 to 0.57 in GLN group and, as opposed, increased from 0.91 to 1.12 in the ALA group. Conclusion: Oral supplementation with the amino acid L-glutamine in overweight and obese humans, for a period of fourteen days, alters the composition of the gut microbiota in a similar way to weight loss
Mestrado
Nutrição
Mestra em Ciências da Nutrição e do Esporte e Metabolismo
Sfarlea, Iulia. "Isolation and characterisation of indigenous actinobacteria from diverse South African environments." Master's thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/16396.
Full textBelyaevskaya, Anna V. "Characterization of T box riboswitch gene regulation in the phylum Actinobacteria." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437725058.
Full textTawfik, Rahmy. "A Novel Approach to the Discovery of Natural Products From Actinobacteria." Scholar Commons, 2017. http://scholarcommons.usf.edu/etd/6766.
Full textMaake, Takalani Whitney. "Development of an actinobacteria based in vitro transcription and translation systems." University of the Western Cape, 2015. http://hdl.handle.net/11394/4750.
Full textHeterologous metagenomic screening strategies have relied largely on the construction of DNA libraries and screening in Escherichia coli to access novel enzymes. There is an increased demand for the identification of novel lignocellulose degrading enzymes with enhanced biochemical properties which are suitable for applications in industrial processes; biofuels being one of them. The use of heterologous gene expression in function based metagenomic studies has resulted in the discovery of enormous novel bioactive compounds. However, there are limitations associated with using E. coli as a heterologous host which does not allow transcription and translation of all genes in the metagenome. E. coli can only express 40% of the environmental DNA because of promoter recognition, codon usage, and host toxicity of gene products. Therefore alternative strategies for expressing or producing novel enzymes are needed, which can also be employed in metagenomic gene discovery. In vitro protein synthesis is an important tool in molecular biology and used to obtain proteins from genes for functional and expression studies. These systems may hold the key to unlock more of the potential in metagenomic DNA. The broader aim of the study is to develop non- E. coli based cell-free protein synthesis systems to further the metagenomics screening. In this study, Rhodococcus erythropolis H8 was evaluated for its suitability in cell-free expression. Crude extracts containing the macromolecular components (70S or 80S ribosomes, tRNAs, initiation, elongation and termination factors) fromR. erythropolis were prepared using existing crude extract based cell-free protein synthesis (CFPS) protocols. Three genes were selected and used as templates for synthesis: cell11, xp12 and acetyl xylan esterase (axe10), all previously isolated from metagenomic libraries screened inE. coli. As judged by zymograms and enzyme assays, all enzymes were successfully expressedfrom their native promoters and in recombinants clones using the PtipA promoter, and wereactive. Furthermore, the amounts of XP12 protein produced using pFos-XP_12 was 1.2mg/mlfrom E. coli and 1.67mg/ml from R. erythropolis CFPS, showing that the R. erythropolismachinery was more efficient in the expression of XP12 than the E. coli machinery. To the best of our knowledge this is the first demonstration of a cell-free expression using an actinomycete.
Lacey, Heather Jane. "Hidden Underworld: A Study Of Secondary Metabolites From Soil-Derived Actinobacteria." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/29524.
Full textElsayed, Somayah Sameer. "Chemical characterisation of microbial natural products from underexplored habitats." Thesis, University of Aberdeen, 2015. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=227107.
Full textMatsuura, Takeshi. "Caracterização taxonomica de actinomicetos endofiticos produtores de antibioticos isolados de cupuaçuzeiro (Theobroma grandiflorum Schum.) : Takeshi Matsuura." [s.n.], 2004. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256663.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Doutorado
Doutor em Ciência de Alimentos
Sanchez, Beatriz Lisboa. "Fungos negros do tegumento de formigas atíneas derivadas e suas interações com actinobactérias /." Rio Claro, 2019. http://hdl.handle.net/11449/181632.
Full textCoorientador: Fernando Carlos Pagnocca
Banca: Derlene Attili de Angelis
Banca: Vania Aparecida Vicente
Resumo: Os fungos negros são conhecidos por apresentarem melanina na parede celular, a qual proporciona resistência a diversos estresses ambientais. Nos últimos anos, foram descobertas várias associações entre formigas e fungos negros da ordem Chaetothyriales. No caso das formigas cultivadoras de fungos (atíneas), tais associações permanecem pouco exploradas. Fungos próximos ao gênero Cyphellophora (ex-Phialophora), encontrados no tegumento de formigas do gênero Apterostigma (uma atínea basal), inibem as actinobactérias simbiontes presentes no mesmo local, sendo considerados nocivos para as colônias dessas formigas. Por outro lado, espécies de fungos negros da mesma ordem foram encontradas em baixa abundância no tegumento de alados de Atta (uma atínea derivada). Nesse contexto, pouco se sabe sobre a abundância, diversidade e relação ecológica desses fungos em outros gêneros de atíneas derivadas. Este trabalho teve como objetivo descrever a presença e relação ecológica dos fungos negros no tegumento desses insetos. Utilizando dois métodos dependentes de cultivo, avaliamos a diversidade de fungos negros do tegumento de Acromyrmex coronatus (cortadeira de folhas) e Trachymyrmex tucumanus (não-cortadeira de folhas). Após o isolamento, os fungos foram purificados e identificados com base no sequenciamento da região ITS e um fragmento do gene tef1. Em seguida, foram realizados testes de co-cultivo in vitro entre quatro actinobactérias frente aos fungos negros obtidos das mesmas espécies de... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Black fungi are known for the presence of melanin in their cell walls, which provides resistance to different types of environmental stresses. Recently, many associations between ants and black fungi in the order Chaetothyriales have been discovered. When it comes to fungus-growing ants (the "attines"), these associations remain underexplored. Fungi related to the genus Cyphellophora (ex-Phialophora), found on the integument of Apterostigma ants (lower attines), inhibit the symbiotic actinobacteria also present on the ant integument, and for that are considered detrimental for the ant colonies. However, black fungi species belonging to the same order were found in low abundance on the integument of gynes and drones of Atta (higher attines). In this context, little is known about the abundance, diversity and ecological relation of these fungi in other genera of derived attines. The aim of this study was to describe the presence and ecological relation of black fungi on the integument of these insects. By applying two different culture dependent methods, we evaluated the diversity of black fungi on the integument of Acromyrmex coronatus (leafcutter) and Trachymyrmex tucumanus (non-leafcutter). After isolation, fungi were purified and identified based on ITS region and partial tef1 gene sequences. Next, we put together in vitro co-culture assays between four actinobacteria and black fungi obtained from the same ant species. We found 111 black fungi in both species of ants, inclu... (Complete abstract click electronic access below)
Mestre
Sottorff-Neculhueque, Ignacio [Verfasser]. "Diversity of Easter Island Actinobacteria and their secondary metabolites / Ignacio Sottorff-Neculhueque." Kiel : Universitätsbibliothek Kiel, 2019. http://d-nb.info/1188612042/34.
Full textSom, Nicolle. "MtrAB-LpqB : a conserved pathway regulating cell division in the phylum Actinobacteria?" Thesis, University of East Anglia, 2016. https://ueaeprints.uea.ac.uk/64007/.
Full textFrança, Aline Giovana da. "Filo Actinobacteria e abundância do gene alkB em solos rizosféricos cultivados sob sistemas de colheita de cana-de-açúcar." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-29112017-102300/.
Full textThe phylum Actinobacteria is currently considered one of the major phyla belonging to the domain Bacteria. This phylum can represent up to 30% of the soil microbial community. The organisms of the phylum Actinobacteria has been characterized as antibiotic producers and degraders of complex substances such as alkanes, present in several substances found in plants. Regarding the degradation of alkanes it has already been proven that alkB gene (alkane hydroxylase), present in the phylum Actinobacteria, is related to its degradation. But the communities of such organisms can be altered by changes in land use, which in Brazil occur extensively due to implementation of monocultures. The cultivation of sugarcane, which has been expanded annually, appears as one of the crops that can alter the soil microbiota. The different sugarcane harvest systems, with and without straw burning, can alter the soil microbiota.Thus, considering the role of the phylum Actinobacteria in the cycling of organic matter, in addition to their biotechnological importance, this study evaluated how different harvest systems of sugarcane influence rhizospheric communities of Actinobacteria and alkane-oxidizing bacteria under controlled conditions in a greenhouse, using molecular techniques to quantify (qPCR), analyse the structure (T-RFLP) and diversity of the community (metagenomic sequencing). For this, the soil collected from sugarcane fields with harvest managements with and without burning the straw, was used in a mesocosms experiment composed of pots with a sugarcane plant, from where rhizosphere soil was collected, and without plant, from where control soil was collected. The quantification of Actinobacteria 16S rRNA gene and alkB gene showed no statistical differences in the comparison of sugarcane controls soil with and without burning, and in the comparison between rhizosphere and control soil of each type of crop management. Terminal Restriction Fragments (TRF) analysis of control soils of different harvesting systems showed a separation of the bacterial community present in the soil, the same occurred in the comparison between rhizosphere and control soils of different harvesting systems. However, when observing the taxonomic structure of the bacterial community it is noted that that the phyla Proteobacteria, Actinobacteria, Firmicutes and Acidobacteria are predominant in different sampled soils, the statistical differences between the control and rhizosphere soil of each treatment are presented from the analysis of bacterial families present in the soil. For alkB gene, the sequences found in different soils belong to the two most abundant phyla in the soil, the phylum Proteobacteria and Actinobacteria, but some sequences belong to unidentified bacteria.Thus, it is concluded that the changes in the structure of the bacterial community present in soil with monoculture of sugarcane are apparent from the analysis of lower phylogenetic groups and the alkane-degrading bacterial communities belong mainly to the phyla Actinobacteria and Proteobacteria, however it is still needed further studies for the classification of unidentified bacteria
Tangeman, Lorraine Susan. "Can Antibiotics From Recently Discovered Marine Actinobacteria Slow the Tide of Antibiotic Resistance?" Wright State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=wright1377522942.
Full textPelser, James Grant. "Screening environmental actinobacteria for antimycobacterial antibiotics and characterisation of Kribbella stellenboschensis sp. nov." Master's thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29202.
Full textNtsaluba, Luvuyo. "Studies on bioflocculant production by a consortium of two bacterial species belonging to the Methylobacterium and Actinobacterium genera." Thesis, University of Fort Hare, 2012. http://hdl.handle.net/10353/482.
Full textBarroso, Keli Cristiane Carvalho. "Interação entre Acanthamoeba polyphaga e Streptomyces sp. em um modelo de cocultivo visando a obtenção de extrato bruto com ação antimicrobiana." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/143018.
Full textThe interactions that occur between bacteria and amoebas can give through mutual relations, where both organisms benefit from the association or parasitic in which one organism benefits at the expense of the other. The coexistence of various microorganisms share the same environment can produce alterations in the growth of the organisms is, patterns of adaptation in morphology, development, or even in their ability to synthesize proteins and secondary metabolites. This study evaluates the interaction between Acanthamoeba polyphaga and Streptomyces sp. through cocultivation, in order to obtain crude extracts with antimicrobial action. In cocultivation, amoebas made it impossible in the presence of the bacteria. After contact with amoebae were morphological changes in Streptomyces sp. All incubation times with hyphae production, different control spores this remained in phase. From the cocultivation it was possible to obtain crude extract in 50 days, being evaluated in different incubation times (1º, 7º, 14º, 21º and 28º days), against multiresistant bacteria such as Escherichia coli and Pseudomonas aeruginosa, showing antimicrobial activity in both the cocultivation and in control. With statistical analysis found that the extracts in 24 hours (1º) showed greater activity, especially against P. aeruginosa. The extracts produced by the coculture and control behaved differently from one another, but the differences were not statistically significant. Regarding the biomass produced, there was a higher volume of biomass in cocultivation, than in the control, indicating that the contact between the two organisms favored the cell mass production, but there was no significant difference only when compared between days. These results show that there is interaction between Acanthamoeba and Streptomyces since the bacteria benefited amoeba assisting in their development. This interaction between microorganisms may be important in modulating the production of antimicrobial substances, a fact that still requires investigation.
Pinheiro, Damasceno Florentino Bruno [Verfasser], and Kirsten [Akademischer Betreuer] Jung. "The translation elongation factor P in actinobacteria / Bruno Pinheiro Damasceno Florentino ; Betreuer: Kirsten Jung." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/1222436736/34.
Full textEverest, Gareth John. "Selective Isolation and Characterization of Indigenous Actinobacteria, with Particular Emphasis on the Genus Amycolatopsis." Doctoral thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/4259.
Full textDavids, Natasha. "An investigation into the enzymatic activity of deepsea actinobacteria in decolourising crystal violet dye." Master's thesis, Faculty of Engineering and the Built Environment, 2019. http://hdl.handle.net/11427/31145.
Full textKing, Maria Catharina. "Bioactive actinobacteria associated with two South African medicinal plants, Aloe ferox and Sutherlandia frutescens." University of Western Cape, 2021. http://hdl.handle.net/11394/8382.
Full textActinobacteria, a Gram-positive phylum of bacteria found in both terrestrial and aquatic environments, are well-known producers of antibiotics and other bioactive compounds. The isolation of actinobacteria from unique environments has resulted in the discovery of new antibiotic compounds that can be used by the pharmaceutical industry. In this study, the fynbos biome was identified as one of these unique habitats due to its rich plant diversity that hosts over 8500 different plant species, including many medicinal plants. In this study two medicinal plants from the fynbos biome were identified as unique environments for the discovery of bioactive actinobacteria, Aloe ferox (Cape aloe) and Sutherlandia frutescens (cancer bush).
Aryal, Niraj [Verfasser]. "Unravelling the Chemical Diversity of Actinobacteria Obtained from Unique Asian Ecological Niches / Niraj Aryal." Tübingen : Universitätsbibliothek Tübingen, 2023. http://d-nb.info/1240673205/34.
Full textOladele, Agunbiade M. "Studies on bioflocculants produced by three freshwater Actinomycetes (Streptomyces Sp.Gansen, Cellulomonas Sp,Bola and Brachybacterium Sp, UFH) isolated from Tyume river." Thesis, University of Fort Hare, 2011. http://hdl.handle.net/10353/6550.
Full textSalvaggio, Flavia. "Synthesis of biologically active quinolone natural products extracted from the actinomycete Pseudonocardia sp. CL38489." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648770.
Full textOmori, Wellington Pine. "Composição funcional e taxonômica de enzimas carbohidrases que atuam na desconstrução da lignocelulose de torta de filtro /." Jaboticabal, 2018. http://hdl.handle.net/11449/152891.
Full textCoorientador: Daniel Guariz Pinheiro
Coorientador: Luciano Takeshi Kishi
Resumo: A torta de filtro apresenta bagaço residual oriundo do processo de extração do caldo de cana-de-açúcar e quando armazenada por longos períodos, se torna um habitat ideal para o desenvolvimento de comunidades microbianas que atuam na desconstrução da lignocelulose. Nossas análises de dados de sequenciamento de DNA metagenômico sugerem que a torta de filtro armazenada por 40 dias possui uma microbiota com características funcionais e ecológicas exclusivas em relação a outros ambientes com elevada disposição de material lignocelulósico. Assim como em ambientes de compostagem, os filos mais abundantes são Actinobacteria, Proteobacteria, Firmicutes e Bacteroidetes. Dentre os principais genes que estes micro-organismos possuem, estão Glicosiltransferases, Carboidrato Esterases e Glicosil Hidrolases, que atuando em conjunto, são passíveis de desconstruírem a lignocelulose e participarem na liberação de açúcares menores, ácidos orgânicos e outros nutrientes. Neste trabalho, identificamos novas enzimas da família AA10 que oxidam a celulose cristalina, demostrando o potencial deste ambiente em possibilitar a adaptação de micro-organismos que expressam enzimas capazes de desestruturar a celulose altamente condensada, possibilitando a liberação de moléculas de glicose. A comunidade microbiana pode acessar nutrientes como Fósforo e Nitrogênio através da despolimerização da biomassa vegetal ou decomposição da microbiota morta. No ciclo biogeoquímico do nitrogênio, a evaporação de amônia é ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The filter cake presents residual bagasse from the process of extracting the sugarcane juice and when stored for long periods, it becomes an ideal habitat for the development of microbial communities that act in the deconstruction of lignocellulose. Our analyzes of metagenomic DNA sequencing data suggest that the filter cake stored for 40 days has a microbiota with unique functional and ecological characteristics compared to other environments with high lignocellulosic material. Thus in composting environments, the most abundant phyla are Actinobacteria, Proteobacteria, Firmicutes, and Bacteroidetes. Glycosyltransferases, Carbohydrate Estersases and Glycoside Hydrolases, which act together, are capable of deconstructing lignocellulose and participate in the release of smaller sugars, organic acids and other nutrients. In this work, we identify new enzymes of the AA10 family that oxidize crystalline cellulose, demonstrating the potential of this environment to enable the adaptation of microorganisms that express enzymes capable of destabilizing highly condensed cellulose, allowing the release of glucose molecules. The microbial community can access nutrients such as Phosphorus and Nitrogen through the depolymerization of the plant biomass or decomposition of the dead microbiota. In the biogeochemical cycle of nitrogen, the evaporation of ammonia is reduced by the assimilation of this substance by the microbial community, and ammonia is produced by ammonification of nitrate and... (Complete abstract click electronic access below)
Doutor
Toyama, Danyelle. "Análise da diversidade microbiana aquática em rios e lagos da região amazônica." Universidade Federal de São Carlos, 2012. https://repositorio.ufscar.br/handle/ufscar/5501.
Full textFinanciadora de Estudos e Projetos
The Amazon region has the largest hydrographic basin on the planet, the one that includes Amazon River, and it also has the largest rainforest in the world. Moreover, it presents a great biological diversity, both related to the fauna and flora, and microbiological. Microorganisms are responsible for most biogeochemical cycles that shape the terrestrial environment and the freshwater and marine ecosystems, and they can be widely exploited biotechnologically. It is estimated that less than 1% of all bacterial species is known due to our inability to simulate the environment in which they live. However, new techniques have allowed the study of these microorganisms. Through Metagenomics it is possible to study complex environmental samples without the need for isolation and individual cultivation of these organisms. For this purpose, the16S ribosomal DNA (16S rDNA) is used in bacteria and archaea in order to study phylogeny and diversity. This sequence is used because it has been fairly maintained during the processes of biological evolution and it may serve as an indicator of how organisms are closely related. For these studies, this region was amplified by the Polymerase Chain Reaction (PCR) and cloned into vectors through the recombinant DNA technology, thus enabling the construction of 16S rRNA libraries. These libraries were then sequenced and the microorganisms were identified by comparison with databases. In this study it was used DNA extracted from Solimões River filtered water, in addition to water from other rivers and adjacent lakes, to the construction of libraries, in order to study the biodiversity through 16S rRNA analysis. In all libraries, phylum Proteobacteria was the most abundant, and most of the genera observed belong to the Betaproteobacteria class. The freshwater cosmopolitan taxa Candidatus Planktophila limnetica and Polynucleobacter were observed, as well as primary producers were represented by the genera Synechococcus and Cyanobium. Samples in which the construction of 16S rRNA libraries was possible for Archaea, the phylum Crenarchaeota was the most abundant in all libraries.
A Região Amazônica apresenta a maior bacia hidrográfica do planeta, a do rio Amazonas, e também a maior floresta tropical do mundo. Além disso, apresenta uma grande diversidade biológica, tanto relacionada à fauna e flora, quanto microbiológica. Os micro-organismos são responsáveis pela maioria dos ciclos biogeoquímicos que moldam o ambiente terrestre e os ecossistemas de água doce e marinhos, e podem ser amplamente explorados biotecnologicamente. Estima-se que menos de 1% de todas as espécies bacterianas seja conhecida, devido à incapacidade de simulação do ambiente em que vivem. Contudo, novas técnicas têm possibilitado o estudo desses micro-organismos. Através do Metagenoma é possível estudar amostras ambientais complexas sem a necessidade de isolamento e cultivo individual desses organismos. Para tanto, utiliza-se, em bactérias e arquéias, o DNA ribossomal 16S (16S rDNA), para fins de estudos de filogenia e diversidade. Esta sequência é utilizada por se ter mantido bastante conservada durante os processos de evolução biológica, podendo servir como um indicador de como os organismos estão intimamente relacionados. Para estes estudos, esta região foi amplificada pela Reação em Cadeia da Polimerase (PCR) e clonada em vetores através da tecnologia do DNA recombinante, possibilitando, assim, a construção de bibliotecas de 16S rRNA. Estas bibliotecas foram então sequenciadas e os micro-organismos identificados por comparação com bancos de dados. Neste trabalho foi utilizado DNA extraído de filtrados de água do Rio Solimões, além de outros rios e lagos adjacentes, para construção de bibliotecas, com a finalidade de estudar a biodiversidade por meio de análise do 16S rRNA. Em todas as bibliotecas o filo Proteobacteria foi o mais abundante, e a maioria dos gêneros observados pertence à classe Betaproteobacteria. Os taxa cosmopolitas de água doce Candidatus Planktophila limnetica e Polynucleobacter foram observados, assim como os produtores primários foram representados pelos gêneros Synechococcus e Cyanobium. Nas amostras em que a construção das bibliotecas de 16S rRNA foi possível para Archaea, obteve-se o filo Crenarchaeota como o mais abundante em todas as bibliotecas.
Minotto, Elisandra. "Caracterização de compostos produzidos por actinomicetos para o biocontrole de Bipolaris sorokiniana." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/97855.
Full textEndophitic actinobacterias are present in plant tissues and by means of active metabolites they protect and help them in stress conditions. These microorganisms have been widely used in the control of phytopathogenic diseases, such as the spot blotch caused by Bipolaris sorokiniana. This fungus is a causal agent of common root rot, leaf spots, death of seedlings and black point of the seeds of wheat and barley, causing significant reduction in productivity. In this context, the aim of this study was to evaluate the virulence of the B. sorokiniana isolates and the antifungal activity of actinobacterias against this phytopathogen. The antagonists with the higher activity against the phytopatogen were characterize taking in consideration their physiology, enzyme production, growth conditions and metabolites production and 16S rRNA sequencing for identification of the antagonists. Partial characterization (nós não purificamos) of the metabolites was performed using thin layer chromatography (TLC) systems containing different solvents. The results showed that the isolates of B. sorokiniana have a high virulence on wheat seed and seedlings, however the greater aggressiveness was observed to seed. On the other hand, 69.6% of actinomycetes showed high antifungal activity against of B. sorokiniana isolates on solid medium, and 17% maintained this behavior in submerged culture. The highest yield happened, for most isolates, when grown at 30°C with agitation after 72h of incubation. The detection of catalase, starch, pectin, lipase and esterase production was observed for most of the actinomycetes (100, 95.6, 91.30, 95.6, 100%, respectively). While the hydrolysis of casein, carboxymethylcellulase and gelatin was performed by 60.8, 34.78 and 47.82% of the isolates, respectively. Isolates 6(2), 6(4), 16(3) e R18(6), selected due to the high antifungal and enzyme activity, showed a positive reaction for the production of volatile compounds, chitinase and glucanase, siderophores, nitrogen fixation, AIA and colonization of the roots. Only the isolated R18(6) showed no ability to solubilize phosphates. Molecular characterization of the isolates determined that they belong to the genus Streptomyces. The metabolites produced by isolate R18 (6) were more stable to temperature and pH changes, as well to the action of proteases and EDTA, when compared to the others. The solvents ethyl acetate and hexane were more efficient for the extraction of the metabolites from the crude extract, however a better separation of the metabolites in the TLC was obtained with mixture of solvents.
Manderscheid, Niko [Verfasser], and Andriy [Akademischer Betreuer] Luzhetskyy. "Strain development for heterologous expression of secondary metabolite clusters in actinobacteria / Niko Manderscheid. Betreuer: Andriy Luzhetskyy." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2016. http://d-nb.info/108253644X/34.
Full textLiu, Jing [Verfasser], and Shu-Ming [Akademischer Betreuer] Li. "Genome mining-directed discovery of novel 2,5-diketopiperazines from actinobacteria / Jing Liu ; Betreuer: Shu-Ming Li." Marburg : Philipps-Universität Marburg, 2021. http://d-nb.info/1230552634/34.
Full textDu, Plessis Gerda. "Actinobacterial diversity of the Ethiopian Rift Valley lakes." University of the Western Cape, 2011. http://hdl.handle.net/11394/5385.
Full textThe class Actinobacteria consists of a heterogeneous group of filamentous, Gram-positive bacteria that colonise most terrestrial and aquatic environments. The industrial and biotechnological importance of the secondary metabolites produced by members of this class has propelled it into the forefront of metagenomics studies. The Ethiopian Rift Valley lakes are characterized by several physical extremes, making it a polyextremophilic environment and a possible untapped source of novel actinobacterial species. The aims of the current study were to identify and compare the eubacterial diversity between three geographically divided soda lakes within the ERV focusing on the actinobacterial subpopulation. This was done by means of a culture-dependent (classical culturing) and culture-independent (DGGE and ARDRA) approach. The results indicate that the eubacterial 16S rRNA gene libraries were similar in composition with a predominance of α-Proteobacteria and Firmicutes in all three lakes. Conversely, the actinobacterial 16S rRNA gene libraries were significantly different and could be used to distinguish between sites. The actinobacterial OTUs detected belonged to both the Rubrobacterales and Actinomycetales orders with members of the genus Arthrobacter being found in all three lakes. Geochemical properties were significantly different between the lakes, although more than one property attributed to the variance between community compositions. The diversity detected in the culture-based study differed significantly and all isolates belonged to the genus Streptomyces. Two novel strains were characterized by means of phylogenetic (16S rRNA gene sequence), physiological, morphological and biochemical analyses. Both novel isolates were capable of growing under "extreme" conditions- pH 12, 10% NaCl and 45°C. Partial enzyme characterization revealed that both strains produced xylanase enzymes that were active at pH 6.5 and 8.5 with an increase in activity up to 45°C. The results obtained revealed a previously undetected diversity of actinobacteria in the Ethiopian Rift Valley with a potentially novel subpopulation adapted to haloalkaline conditions. The low 16S rRNA sequence similarity of a substantial proportion of the libraries suggests that culture-based isolation may play a vital role in deciphering the community fingerprint.
The National Research Foundation and the Norwegian Research Council
Costa, Luiz Antonio Mendonça Alves da. "Reações de oxidação e hidrolise por microrganismos nos metodos de biocatalise e de biorremediação." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/249096.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Quimica
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Resumo: O presente trabalho foi dividido em dois projetos: a avaliação do potencial biocatalítico de microrganismos isolados da abelha Trigonna sp e o estudo de biorremediação de ambiente contaminado por Alachlor®. A atividade catalítica de oxidação de sulfeto e a hidrólise de éster sulfínico de 12 linhagens de fungos foi avaliada durante a primeira parte deste trabalho, dentre das quais, 8 linhagens foram isoladas do corpo da abelha Trigonna sp em trabalhos anteriores do nosso grupo. Os melhores microrganismos na oxidação enantiosseletiva do etil fenil sulfeto foram os Fungo CCT 5553 e Cladosporium sp. CBMAI 0210 que produziram o (S)-etil-fenil-sulfóxido (ee > 99%) e (R)-etil-fenil-sulfóxido (ee 97%), respectivamente. O (S)-etil-fenil-sulfóxido (ee > 92%) foi aplicado na síntese da S-(+)-4-metil-3-heptanona, feromônio de alarme da formiga do gênero Atta, mas uma racemização durante a eliminação do grupo sulfinila impossibilitou a síntese total. Para a resolução enzimática de (±)-benzenossulfinato de cicloexila foi selecionado os fungos Penicillium sp. CBMAI 0208 e Aspergillus ochraceus CBMAI 0211 ambos fornecendo os produtos com excessos enantioméricos > 99%. Na segunda etapa, avaliou-se a capacidade de degradação do pesticida Alachlor® de 6 linhagens de bactérias (Streptomyces sp.) e as estruturas dos produtos de degradação foram sugeridas baseados em seus padrões de fragmentação. Entre esses 8-etil-quinolina e N-metil-8-etil-indol nunca foram citados nos estudos de biodegradação do Alachlor®.
Abstract: The work presented in this thesis is divided into two projects: the evaluation of the biocatalytic potential of microorganisms isolated from Trigonna bee and those deposited in two brazilian collections and bioremediation of Alachlor contamined soil. The sulfide oxidation and sulfinic esters hydrolysis catalytic activity was screened using 12 different fungi strains, 8 of which were previously isolated from a Trigonna sp. bee. The best microorganisms for the enantioselective oxidation of ethyl phenyl sulfide were Fungus CCT 5553 and Cladosporium sp. CBMAI 0210 WHICH PRODUCED (R)-ethyl phenyl sulfoxide (ee 97%) and (S)-ethyl phenyl sulfoxide (ee > 99%) respectively. The chiral (S)-ethyl phenyl sulfoxide which was applied in the synthesis of S-(+)-4-methyl-3-heptanone, ant alarm pheromone (genus Atta), of but racemization during sulfinyl group elimination step precluded the total asymmetric synthesis. For the enzymatic resolution of the cyclohexyl (±)-benzenosulfinate we have selected Penicillium sp. CBMAI 0208 and Aspergillus ochraceus CBMAI 0211 both with the capacity of resolving the sulfinate in over 99 enantiomeric excess. In the second part, the Alachlor® degradation potential of 6 bacterium strains (Streptomyces sp.) was evaluated and the structures of biodegradation products were suggested based on their mass fragmentation patterns. Among these 8-ethyl-quinoline and N-methyl-8-ethyl-indole have never been mentioned as Alachlor biodegradation products before.
Doutorado
Quimica Organica
Doutor em Quimica
Carvalho, Tiele da Silva. "Avaliação de atividade antibacteriana do actinomiceto endofítico R18(6) contra bactérias gram-negativas multirresistentes." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/108934.
Full textGram-negative bacteria of the Enterobacteriaceae and Pseudomonadacea family are the most common pathogens isolated from infections. Due to the increase of microorganisms resistant to antimicrobial agents available for treatment, the search for new compounds, mainly from natural and culturable sources, has become an important issue. The actinomycetes are a major source of secondary metabolites with antibacterial activity. The aim of this work was to evaluate the potential production of active metabolites by the endophytic actinomycete R18(6) against Gram-negative bacteria multiresistant. For this, the double layer method was used to assess the ability of production of active metabolites by the isolate. Based on this assay the culture condition growth of the isolate in submerged culture was optimized. For that as carbon source, temperature, pH, incubation way and incubation time were tested looking for a better metabolite production. The antimicrobial activity of the isolate was evaluated every 24 hours for 10 days by the well diffusion assay, where the inhibition halo was measured. The actinomycete showed the best activity against Gram-negative bacteria when cultured in base medium supplemented with glucose, adjusted in pH 6,5, incubation temperature of 30ºC for 96 hours with agitation. In the microdilution assay the concentration of crude extract varied from 1/32 to 1/256, and it showed bactericidal or bacteriostatic activity according to Gram-negative tested isolate. The thermal and enzymatic stability of crude extract were evaluated, where it exhibited thermal stability in high temperature and it was unstable to proteolytic enzymes. The crude extract was subjected to solvent extraction and acetone was efficient as extractor solvent. The isolate showed similar characteristics of the genus Streptomyces when evaluated by optical and scanning microscopy. The endophytic actinomycete R18(6) showed to be a new and a promising source of active metabolites production against Gram-negative bacteria multidrug resistant.
Borba, Marcela Proença. "Caracterização de isolados de actinobactérias utilizando BOX-PCR e URP-PCR e purificação de composto bioativo produzido por um isolado de Streptomyces sp." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/143012.
Full textThe Actinobacteria phylum is an important group of Gram positive bacteria widely distributed in terrestrial and aquatic environments. They are major producers of biologically active compounds and therefore of great biotechnological interest. The genus Streptomyces stands out as the largest producer of these compounds, accounting for about 70% of the antibiotics that we use today. The identification of these organisms is still a challenge. For many years the identification was carried out only based on morphological and physiology characteristics. Today, with the advance of molecular techniques, there are a large number of species reported in genomics database. This work aims to identify isolates of actinobacterias present in the Laboratório de Microbiologia Ambiental ICBS / UFRGS with the help of BOX-PCR techniques, widely used in diversity studies within this phylum, and URP-PCR. And besides that, performing partial purification of an antimicrobial compound effective against bacteria produced by Streptomyces 8S isolated. The URP and BOX1AR primers produced different amplification patterns in the isolates, but it was not possible to investigate the relationship of similarity between them. Also the sequencing of 16S rDNA was not efficient to identify the species. To purify the antimicrobial compound was carried out liquid-liquid extraction with the solvent ethyl acetate, subsequently chromatography: gel-filtration (Sephadex G-75) and ion exchange (SP-Sepharose and DEAE-cellulose). The antimicrobial in question did not adhere to the SP-Sepharose column, showing that it has negative charge. The antimicrobial activity of the compound was recovered after each step. The compound remained active after the stability tests like the addition of EDTA, proteolytic enzymes and high temperatures. This suggests that the antimicrobial compound is not a protein. This work suggests further studies based on the obtained preliminary results such as the amplification of the entire 16S rDNA of actinobacterias isolated fragment and the investigation of the antimicrobial compound using high resolution chromatography.
Pereira, Priscila Monteiro. "Avaliação da atividade antifúngica sobre Bipolaris sorokiniana e promoção de crescimento em plantas de trigo de isolados de Streptomyces sp." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2017. http://hdl.handle.net/10183/165282.
Full textStreptomyces sp. R18(6) and 6(4) strains were evaluated for their ability to control brown spot and common root rot caused by Bipolaris sorokiniana in wheat crops. The antifungal activity of these isolates was tested using a doublelayer assay and culture pairing at 28 °C. Physiological and enzymatic activity were evaluated through siderophore, indole-3-acetic acid, nitrogen fixation and phosphate solubilization assays. The biocontrol of the disease and growthpromoting efficiency of wheat seedlings were assessed using in vivo assays in greenhouse. In the culture pairing assays, both strains inhibited B. sorokiniana mycelial growth, while in the double-layer only R18(6). Streptomyces sp. 6(4) produced auxin, siderophores, fixed nitrogen and solubilized phosphate, whereas R18(6) did not produce siderophores. In the greenhouse assays, strain R18 (6) showed statistical differences in shoot dry mass and root dry mass compared with those of strain 6(4) in the presence of the phytopathogen (P ≤ 0.05). These results were more evident when the temperature was higher. In the absence of the phytopathogen, strain 6(4) increased the root dry mass compared with that of the control during the same period. Therefore, these isolates can potentially control root rot and brown spotting and may promote the growth of wheat plants.
Salem, Shaimaa Mohamed. "Biosynthesis of Marineosin, a Spiroaminal Undecylprodiginine Natural Product." PDXScholar, 2012. https://pdxscholar.library.pdx.edu/open_access_etds/936.
Full textLandwehr, Wiebke Verfasser], and Joachim Manfred [Akademischer Betreuer] [Wink. "Isolation and characterization from novel actinobacteria and myxobacteria especially from marine habitats / Wiebke Landwehr ; Betreuer: Joachim M. Wink." Braunschweig : Technische Universität Braunschweig, 2017. http://d-nb.info/1175817252/34.
Full textLandwehr, Wiebke Verfasser], and Joachim [Akademischer Betreuer] [Wink. "Isolation and characterization from novel actinobacteria and myxobacteria especially from marine habitats / Wiebke Landwehr ; Betreuer: Joachim M. Wink." Braunschweig : Technische Universität Braunschweig, 2017. http://nbn-resolving.de/urn:nbn:de:gbv:084-2017071911011.
Full text