Relevant bibliographies by topics / Actinobacillus pleuropneumoniae

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Academic literature on the topic 'Actinobacillus pleuropneumoniae'

Author: Grafiati
Published: 4 June 2021
Last updated: 20 February 2023

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Journal articles on the topic "Actinobacillus pleuropneumoniae"

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Andrusevich, A. S., E. L. Krasnikova, M. M. Misteyko, I. I. Strelchenya, M. S. Struk, and O. V. Malchik. "BIOCHEMICAL PROPERTIES OF ACTINOBACILLUS PLEUROPNEUMONIAE MUSEUM STRAINS." Ecology and Animal World, no. 1 (May 28, 2021): 58–62. http://dx.doi.org/10.47612/2224-1647-2021-1-58-62.

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The article provides data on the biochemical properties of museum strains of Actinobacillus pleuropneumoni-ae. The belonging of the strains of Actinobacillus pleuropneumoniae was confirmed in the polymerase chain reaction using the developed RUE «Institute of experimental veterinary medicine nam. of S.N. Wyshelessky» test system for detecting the genome of Actinobacillus pleuropneumoniae.
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Rusaleyev, V. S. "ANTIBIOTIC RESISTANCE OF ACTINOBACILLUS PLEUROPNEUMONIAE IN SWINE: PROBLEMS AND SOLUTIONS." Veterinary Science Today, no. 3 (October 3, 2018): 26–29. http://dx.doi.org/10.29326/2304-196x-2018-3-26-26-29.

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Porcine pleuropneumonia is an infectious contagious disease caused by bacteria Actinobacillus pleuropneumoniae. Currently, the disease is widespread in many countries with well-developed pig production. The disease causes significant economic damage to farms due to the large mortality and expenses for treatment of diseased pigs and implementation of veterinary and sanitary measures. Due to increased number of Actinobacillus pleuropneumoniae cases in pigs, and the emergence of actinobacillus-resistant forms, it is necessary to perform a more thorough study and discussion of this problem. The disease epidemic surveillance is based on continuous monitoring aimed at porcine Actinobacillus pleuropneumoniae identification, confirmation and registration, determination of its characteristics and trends in development of sensitivity to antimicrobial preparations. The article addresses the topic of antibiotic use and the antibiotic resistance of microorganisms, which is actual not only for veterinary medicine but also for medicine. The model of swine Actinobacillus pleuropneumoniae was used to study the reasons of antibiotic resistance. Possible approaches to overcoming the resistance of actinobacilli to antibiotics have been discussed. The prospects for the use of antibiotics were discussed in detail to cope with this problem. Targeted surveillance, aimed at monitoring and collecting information on the prescription of antibiotics is of great importance for the solution of the problem of antibiotic resistance. The information obtained from the monitoring can be used for development of the plan and strategy for the use of antibacterial preparations (preparation selection, dose, route of administration, frequency, number of courses), development and implementation of more effective approaches to the treatment of Actinobacillus pleuropneumoniae in pigs, control of the antibiotic-resistant bacteria occurrence and spread.
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Subashchandrabose, Sargurunathan, Rhiannon M. Leveque, Roy N. Kirkwood, Matti Kiupel, and Martha H. Mulks. "The RNA Chaperone Hfq Promotes Fitness of Actinobacillus pleuropneumoniae during Porcine Pleuropneumonia." Infection and Immunity 81, no. 8 (June 3, 2013): 2952–61. http://dx.doi.org/10.1128/iai.00392-13.

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ABSTRACTActinobacillus pleuropneumoniaeis the etiological agent of porcine pleuropneumonia, an economically important disease of pigs. Thehfqgene inA. pleuropneumoniae, encoding the RNA chaperone and posttranscriptional regulator Hfq, is upregulated during infection of porcine lungs. To investigate the role of thisin vivo-induced gene inA. pleuropneumoniae, anhfqmutant strain was constructed. Thehfqmutant was defective in biofilm formation on abiotic surfaces. The level ofpgaCtranscript, encoding the biosynthesis of poly-β-1,6-N-acetylglucosamine (PNAG), a major biofilm matrix component, was lower and PNAG content was 10-fold lower in thehfqmutant than in the wild-type strain. When outer membrane proteins were examined, cysteine synthase, implicated in resistance to oxidative stress and tellurite, was not found at detectable levels in the absence of Hfq. Thehfqmutant displayed enhanced sensitivity to superoxide generated by methyl viologen and tellurite. These phenotypes were readily reversed by complementation with thehfqgene expressed from its native promoter. The role of Hfq in the fitness ofA. pleuropneumoniaewas assessed in a natural host infection model. Thehfqmutant failed to colonize porcine lungs and was outcompeted by the wild-type strain (median competitive index of 2 × 10−5). Our data demonstrate that thein vivo-induced genehfqis involved in the regulation of PNAG-dependent biofilm formation, resistance to superoxide stress, and the fitness and virulence ofA. pleuropneumoniaein pigs and begin to elucidate the role of anin vivo-induced gene in the pathogenesis of pleuropneumonia.
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To, Ho, Kaho Teshima, Michiha Kon, Saori Yasuda, Yuta Akaike, Kazumoto Shibuya, Shinya Nagai, and Chihiro Sasakawa. "Characterization of nontypeable Actinobacillus pleuropneumoniae isolates." Journal of Veterinary Diagnostic Investigation 32, no. 4 (June 9, 2020): 581–84. http://dx.doi.org/10.1177/1040638720931469.

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Two Actinobacillus pleuropneumoniae isolates from clinical cases of porcine pleuropneumonia in Japan were positive in the capsular serovar 15–specific PCR assay, but nontypeable (NT) in the agar gel precipitation (AGP) test. Nucleotide sequence analysis of gene clusters involved in the biosynthesis of capsular polysaccharide (CPS) and lipopolysaccharide O-polysaccharide (O-PS) revealed that both clusters contained transposable element IS Apl1 of A. pleuropneumoniae belonging to the IS30 family. Immunoblot analysis revealed that these 2 isolates could not produce O-PS. We conclude that the IS Apl1 of A. pleuropneumoniae can interfere in the biosynthesis of both CPS and O-PS.
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Frank, Rodney K., M. M. Chengappa, Richard D. Oberst, Kristina J. Hennessy, Steven C. Henry, and Brad Fenwick. "Pleuropneumonia Caused by Actinobacillus Pleuropneumoniae Biotype 2 in Growing and Finishing Pigs." Journal of Veterinary Diagnostic Investigation 4, no. 3 (July 1992): 270–78. http://dx.doi.org/10.1177/104063879200400308.

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Actinobacillus pleuropneumoniae biotype 2 was isolated in pure culture or as the predominant isolate from the lungs of 9 growing and finishing pigs with pleuropneumonia. Gross and microscopic lesions resembled those caused by A. pleuropneumoniae biotype 1 serotypes (nos. 1, 5, and 7) traditionally seen in the United States. The overall mortality rate for growing and finishing pigs on this 1,200-sow far-row-to-finish farm ranged from 0.37% to 0.84% per month from July 1990 to February 1991, and mortality due to respiratory disease ranged from 0.17% to 0.52% per month for the same period. This Actinobacillus species did not require V factor (no satellitism on blood agar with a Staphylococcus streak), was strongly beta-hemolytic, and demonstrated restriction fragment length polymorphisms in hybridization studies with A. suis, A. lignieresii, and A. equuli. Biochemically, the isolate most closely resembled A. pleuropneumoniae, and a DNA fragment considered specific for A. pleuropneumoniae biotypes 1 and 2 was demonstrated using polymerase chain reaction. Necrohemorrhagic pleuropneumonia similar to that caused by A. pleuropneumoniae biotype 1 was reproduced experimentally in 2 4-week-old pigs inoculated intratracheally with broth cultures of the A. pleuropneumoniae biotype 2. This study demonstrated the presence of A. pleuropneumoniae biotype 2 in the United States.
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Zhou, Yang, Lu Li, Zhaohui Chen, Hong Yuan, Huanchun Chen, and Rui Zhou. "Adhesion Protein ApfA of Actinobacillus pleuropneumoniae Is Required for Pathogenesis and Is a Potential Target for Vaccine Development." Clinical and Vaccine Immunology 20, no. 2 (December 26, 2012): 287–94. http://dx.doi.org/10.1128/cvi.00616-12.

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ABSTRACTActinobacillus pleuropneumoniaeis the etiologic agent of porcine pleuropneumonia, which causes serious economic losses in the pig farming industry worldwide. Due to a lack of knowledge of its virulence factors and a lack of effective vaccines able to confer cross-serotype protection, it is difficult to place this disease under control. By analyzing its genome sequences, we found that type IV fimbrial subunit protein ApfA is highly conserved among different serotypes ofA. pleuropneumoniae. Our study shows that ApfA is an adhesin since its expression was greatly upregulated (135-fold) upon contact with host cells, while its deletion mutant attenuated its capability of adhesion. The inactivation ofapfAdramatically reduced the ability ofA. pleuropneumoniaeto colonize mouse lung, suggesting thatapfAis a virulence factor. Purified recombinant ApfA elicited an elevated humoral immune response and conferred robust protection against challenges withA. pleuropneumoniaeserovar 1 strain 4074 and serovar 7 strain WF83 in mice. Importantly, the anti-ApfA serum conferred significant protection against both serovar 1 and serovar 7 in mice. These studies indicate that ApfA promotes virulence through attachment to host cells, and its immunogenicity renders it a promising novel subunit vaccine candidate against infection withA. pleuropneumoniae.
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Jeannotte, Marie-Eve, Maan Abul-Milh, J. Daniel Dubreuil, and Mario Jacques. "Binding of Actinobacillus pleuropneumoniae to Phosphatidylethanolamine." Infection and Immunity 71, no. 8 (August 2003): 4657–63. http://dx.doi.org/10.1128/iai.71.8.4657-4663.2003.

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ABSTRACT The gram-negative bacterium Actinobacillus pleuropneumoniae is the causative agent of porcine fibrinohemorrhagic necrotizing pleuropneumonia, a disease that causes important economic losses to the swine industry worldwide. In general, the initial step of bacterial colonization is attachment to host cells. The purpose of the present study was to evaluate the binding of A. pleuropneumoniae serotype 1 to phospholipids, which are the major constituents of biological membranes. Phospholipids serve as receptors for several bacteria, including respiratory pathogens. To study this effect, we used thin-layer chromatography overlay binding assays to test commercial phospholipids such as phosphatidic acid, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, and phosphatidylethanolamine (PE). Our results indicate that A. pleuropneumoniae serotype 1 binds to PE but not to the other phospholipids tested. Serotypes 5b and 7, which, along with serotype 1, are the most prevalent serotypes of A. pleuropneumoniae in North America, share the ability to bind PE. Inhibition of binding with a monoclonal antibody against A. pleuropneumoniae serotype 1 O antigen and the use of isogenic lipopolysaccharide (LPS) mutants of A. pleuropneumoniae serotype 1 showed that the O antigen seems to be implicated in the binding to PE, at least for A. pleuropneumoniae serotype 1. A. pleuropneumoniae was also shown to bind to a phospholipid extracted from swine lungs by using the method of Folch. Chemical staining with molybdenum blue and ninhydrin, migration with neutral, acidic, and basic solvent systems, and mass spectrometry analysis all indicated that this lipid is PE. This study is, to the best of our knowledge, the first description of A. pleuropneumoniae binding to phospholipids. Our data also suggest that LPS O antigens could be involved in binding to PE.
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Leiner, Gero, Burkart Franz, Katrin Strutzberg, and Gerald-F. Gerlach. "A Novel Enzyme-Linked Immunosorbent Assay Using the RecombinantActinobacillus pleuropneumoniae ApxII Antigen for Diagnosis of Pleuropneumonia in Pig Herds." Clinical Diagnostic Laboratory Immunology 6, no. 4 (July 1, 1999): 630–32. http://dx.doi.org/10.1128/cdli.6.4.630-632.1999.

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ABSTRACT For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.
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Altman, Eleonora, Douglas W. Griffith, and Malcolm B. Perry. "Structural studies of the O-chains of the lipopolysaccharides produced by strains of Actinobacillus (Haemophilus) pleuropneumoniae serotype 5." Biochemistry and Cell Biology 68, no. 11 (November 1, 1990): 1268–71. http://dx.doi.org/10.1139/o90-188.

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The water-phase lipopolysaccharides isolated by phenol–water extraction from the cells of four strains of Actinobacillus pleuropneumoniae serotype 5 (K17, L20, B78-3760, and 81-750) were shown on structural analysis to have O-chain components with the same basic polysaccharide structure of a linear unbranched homopolymer of 1,6-linked β-D-galactopyranosyl residues, although the linear length of the O-chain varied among different strains. While those of strains B78-3760 and 81-750 were partially O-acetylated, the O-chains of strains K17 and L20 were unsubstituted.Key words: Actinobacillus pleuropneumoniae, polysaccharide, lipopolysaccharide, pleuropneumonia.
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Sárközi, Rita, László Makrai, and László Fodor. "Identification of a proposed new serovar of Actinobacillus Pleuropneumoniae: Serovar 16." Acta Veterinaria Hungarica 63, no. 4 (December 2015): 444–50. http://dx.doi.org/10.1556/004.2015.041.

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Five Actinobacillus pleuropneumoniae strains isolated from pathological lesions of porcine pleuropneumonia in Hungary could not be assigned to any of the accepted 15 serovars. Using hyperimmune serum raised against these unty-pable-serovar A. pleuropneumoniae strains in rabbits, indirect haemagglutination tests proved that they form a distinct group and there is no cross-reaction between them and the type strains of A. pleuropneumoniae. All five strains harboured the toxin-associated genes for the production (apxIA) and secretion (apxIB) of ApxI, the gene for the expression of ApxII and the largest-size (2800 bp) apxIV gene. The carbon source utilisation pattern and the sequence analysis of the 16S rRNA gene confirmed the species identification of the suggested type strain, A. pleuropneumoniae A-85/14. A new serovar of A. pleuropneumoniae — serovar 16 — is proposed with A. pleuropneumoniae A-85/14 as reference strain.
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Dissertations / Theses on the topic "Actinobacillus pleuropneumoniae"

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D'Silva, Colin Gerard. "Iron acquisition by Actinobacillus pleuropneumoniae." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=28720.

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Four strains of the swine pathogen Actinobacillus pleuropneumoniae, namely, the type strain (ATCC 27088), the "reference" strain of biotype 2 (Bertschinger 2008/76) and two additional biotype 1 strains, strain BC181, which is less virulent than the type strain, and strain K17 (reference strain of serotype 5A), which was isolated from a lamb, were investigated with respect to iron acquisition. All four strains produced iron-repressible outer membrane proteins. However, only strains ATCC 27088 and Bertschinger 2008/76 could acquire iron from porcine transferrin. No organism could utilize human, bovine or ovine transferrin, or ovine or porcine lactoferrin. Haemoglobin supported good growth of all strains except K17 (which also failed to acquire iron from haemin). In all cases, iron acquisition from transferrin or haemoglobin required direct contact between the organisms and the proteins. Total membranes derived from iron-restricted organisms were subjected to an affinity isolation technique based on biotinylated porcine transferrin and streptavidin-agarose, and the following polypeptides were isolated: 99 kDa and 64 kDa from strain ATCC 27088; 93 kDa from strain Bertschinger 2008/76; 95 kDa (trace amounts) and 60 kDa from strain BC181; none from strain K17. These polypeptides appear to be transferrin receptor components. The 99 kDa polypeptide (TBPl) from the type strain was purified by SDS-PAGE and transferred electrophoretically onto polyvinylidene difluoride membrane. The N-terminal amino acid sequence of the polypeptide was determined commercially. A commercially-synthesized oligonucleotide probe was used to clone the gene encoding the TBPl of the type strain in competent Escherichia coli DH5$ alpha$ cells.
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Maas, Alexander. "DIVA vaccine development against Actinobacillus pleuropneumoniae infection." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=983732574.

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MacKay, David Keith John. "Immunity to 'Actinobacillus (Haemophilus) pleuropneumoniae' in piglets." Thesis, Royal Veterinary College (University of London), 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.519530.

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Ali, Tehmeena. "Characterisation of protein secretion systems of Actinobacillus pleuropneumoniae." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440122.

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Stäger, Martin. "Zur Seroprävalenz Actinobacillus pleuropneumoniae (APP) in Schweizer Schweinezuchtbeständen /." Bern, 1991. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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Silva, Thyara Ferreira da. "Caracterização e expressão da chaperona Hfq em Actinobacillus pleuropneumoniae, o agente causal da pleuropneumonia suína." Universidade Federal de Viçosa, 2016. http://www.locus.ufv.br/handle/123456789/10060.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico
Infecções que acometem o trato respiratório de suínos levam a significativas perdas econômicas na suinocultura mundial. Um dos principais patógenos respiratórios em suínos é a bactéria Actinobacillus pleuropneumoniae, o principal agente causal da pleuropneumonia. Atualmente podem ser encontrados 16 sorotipos de A. pleuropneumoniae com virulência distinta e complexa, sendo os principais fatores de virulência: exotoxinas Apx, lipopolissacarídeo (LPS), cápsula e a capacidade de formação de biofilme. O controle da doença é baseado no uso de antibióticos e cuidados no manejo da granja. A profilaxia pela imunização passiva ainda é ineficiente devido à dificuldade na obtenção de uma vacina contra todos os sorotipos encontrados. Assim, novas abordagens experimentais na elaboração de uma vacina eficiente associada a uma resposta imune protetora são essenciais porque podem representar novas alternativas na estratégia de controle da doença. A proteína Hfq é um componente central de regulação global pós-transcricional e participa diretamente na regulação da expressão de genes por facilitar a interação de RNAs pequenos com mRNAs alvos, sendo esta uma abordagem atual e relacionada ao controle da virulência em diversas bactérias patogênicas. Neste sentido, o estudo da chaperona de RNA Hfq é de extrema importância, uma vez que já foi demonstrado seu efeito pleiotrópico e impacto na virulência, na resposta a diferentes tipos de estresse e no crescimento celular de vários patógenos, incluindo A. pleuropneumoniae. Portanto, esse trabalho teve como objetivos: caracterizar in silico a proteína Hfq em A. pleuropneumoniae e analisar a expressão e a fase de maior abundância desta proteína ao longo do crescimento de A. pleuropneumoniae. As análises filogenéticas realizadas foram baseadas em análises de comparação de sequências de aminoácidos da proteína Hfq de diferentes membros da classe Gammaproteobacteria, na qual A. pleuropneumoniae está inserida. As demais análises foram conduzidas utilizando os sorotipos 1, 8 e 15 de A. pleuropneumoniae, sendo utilizadas as linhagens do tipo selvagem (WT) e hfq::3XFLAG. O alinhamento de sequências da proteína Hfq revelou uma identidade de 98% entre as proteínas Hfq de A. pleuropneumoniae de diferentes sorotipos, além de demonstar que Hfq de espécies de uma mesma família possuem maior relação filogenética. A análise da estrutura tridimensional da proteína em A. pleuropneumoniae demonstrou a presença de estruturas características da proteína presente em outos patógenos Gram-negativos, como uma α-hélice e folhas β. A análise da velocidade específica de crescimento por ANOVA entre as linhagens WT e hfq::3XFLAG do mesmo sorotipo revelou que não há diferença de crescimento entre essas linhagens do mesmo sorotipo. Quanto à expressão de Hfq, foi detectado um maior acúmulo da proteína na fase estacionária de crescimento, no período de 6-8 horas, dependendo do sorotipo investigado, e que a expressão de Hfq foi diferencial entre os sorotipos analisados. Esses resultados revelaram que a proteína Hfq é conservada entre os sorotipos de A. pleuropneumoniae e possui estrutura tridimensional característica. Além disso, a inserção da etiqueta FLAG em Hfq não alterou o perfil de crescimento celular e hà um maior acúmulo da proteína na fase estacionária de crescimento, sendo que os sorotipos apresentaram distribuição das formas diferencial entre os sorotipos e dinâmica de acordo com a fase de crescimento. Essa diferença pode estar relacionada aos diferentes perfis de virulência e de resposta a diferentes condições investigadas previamente, uma vez que a abundância nestes sorotipos apresentou distribuição temporal distinta.
Infections that affect the respiratory tract of pigs lead to significant economic losses in the swine industry worldwide. One of the major respiratory pathogen in pigs is the bacterium Actinobacillus pleuropneumoniae, the main causal agent of the pleuropneumonia. Currently, 16 serotypes can be found of A. pleuropneumoniae with distinct and complex virulence, with the main factors of virulence: exotoxin Apx, lipopolysaccharide (LPS), capsule and the biofilm formation capacity. Control of the disease is based on the use of antibiotics and care in the management of the farm. Prophylaxis by passive immunization is still inefficient because of the difficulty in getting a vaccine against all serotypes found. Thus, new experimental approaches in the development of an effective vaccine associated with a protective immune response are essential because they can represent new alternatives in the disease control strategy. The Hfq protein is a key component of the global post-transcriptional regulation and directly participates in the regulation of gene expression to facilitate the interaction of small RNAs with target mRNAs, which is a current approach and it relates to the control of virulence in many pathogenic bacteria. In this sense, the study of Hfq RNA chaperone is of extreme importance, since it has already demonstrated its pleiotropic effect and impact on virulence in response to different types of stress and cellular growth of various pathogens, including A. pleuropneumoniae. Therefore, this study aimed: to characterize in silico the Hfq protein in A. pleuropneumoniae and to analyze the expression and phase greater abundance of this protein throughout the growth of A. pleuropneumoniae. The phylogenetic analyzes were based on comparative analysis of amino acid sequences of protein Hfq of different members of the class Gammaproteobacteria, which A. pleuropneumoniae is inserted. The other analyzes were conducted using the serotypes 1, 8 and 15 of A. pleuropneumoniae, being used strains of wild-type (WT) and hfq::3XFLAG. The sequence alignment of Hfq protein sequences showed an identity of 98% between Hfq proteins of A. pleuropneumoniae of different serotypes, also demonstrating that Hfq species of the same family have a greater phylogenetic relationship. The analysis of the three-dimensional structure of the protein in A. pleuropneumoniae demonstrated the presence of specific structures of protein present in other Gram-negative pathogens, how one α-helix and β-strands. The analysis of the specific growth rate by ANOVA between strains WT and hfq::3XFLAG of the same serotype showed that there is no difference in growth between the strains. As the expression of Hfq, a greater accumulation of protein in the stationary growth phase was detected in the period of 6-8 hours, depending on the serotype investigated, and the expression of Hfq was differential between serotypes analyzed. These results demonstrate that Hfq is conserved among serotypes of A. pleuropneumoniae and has a three-dimensional structure conserved. Moreover, the insertion of the FLAG tag on Hfq did not affect the cell growth profile and there is a greater accumulation of the protein in the stationary phase of growth, whereas serotypes showed the distribution of forms differential between serotypes and dynamically according to the growth stage. This difference may be related to different profiles of virulence and response to different conditions previously investigated, since these abundant serotypes showed distinct temporal distribution.
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Ma, Jianneng. "Purification, serology and pathogenic role of the 110 kilodalton rtx hemolysins of Actinobacillus pleuropneumoniae." Diss., Virginia Tech, 1991. http://hdl.handle.net/10919/39935.

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Actinobacillus pleuropneumoniae is the etiological agent of contagious swine pleuropneumonia, an economically important disease of the swine industry worldwide. Improved control of this disease requires enhanced understanding of the factors contributing to pathogenesis. The objectives of this study were to investigate the immune response and virulence properties of the 110-kilodalton (110-KDa) hemolysins [hemolysin I (HlyI) and hemolysin II (HlyII)] of A. pleuropneumoniae. Several monoclonal antibodies (MAb) to the hemolysins were developed. An IgGl. MAb (8C2) specific for HlyII, as determined by immunoblotting, was cross-linked to Protein A-Sepharose, and HlyII was purified from serotypes 1 and 5 by immunoaffinity chromatography. An indirect enzyme-linked immunosorbent assay (ELISA) using MAb 8C2, or affinitypurified rabbit IgG to both hemolysins, was developed for detection of swine antibody to one or both hemolysins, respectively. In comparison with the complement fixation test, the ELISA was highly sensitive and specific, and was able to identify animals infected with or exposed to most, if not all, serotypes of A. pleuropneumoniae. Several nonhemolytic mutants of A. pleuropneumoniae serotype 5 were isolated following electroporation of the parent with an hemolysin gene whose open-reading-frame was disrupted with a kanamycin resistance gene. One mutant was characterized for phenotypic and pathogenic properties. Biochemical profiles, growth rate, capsule content, and lipopolysaccharide and whole cell protein electrophoretic profiles of the parent and one of the mutants were similar. The nonhemolytic mutant lacked both HlyI and HlyII proteins in culture supernatant and in whole cell lysates as determined by immunoblot analysis; extracellular and intracellular hemolytic and cytotoxic activity was also absent. The mutant was avirulent in mice and pigs at doses greater than 10 times the lethal dose of the parent. Unlike the parent, the nonhemolytic mutant failed to confer protection against lethal challenge in mice following immunization. Thus, one or both hemolysins are essential for virulence and immunoprotection in A. pleuropneumoniae serotype 5.
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8

Carufel, Karine de. "Mutagenèse par la technologie du transposome chez Actinobacillus pleuropneumoniae /." Thèse, Trois-Rivières : Université du Québec à Trois-Rivières, 2008. http://www.uqtr.ca/biblio/notice/resume/30037972R.pdf.

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Carufel, Karine de. "Mutagenèse par la technologie du transposome chez Actinobacillus pleuropneumoniae." Thèse, Université du Québec à Trois-Rivières, 2008. http://depot-e.uqtr.ca/1839/1/030037972.pdf.

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Costa, Bárbara Letícia Pereira. "Caracterização fenotípica e genotípica de isolados de Actinobacillus pleuropneumoniae provenientes de diferentes estados brasileiros." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-27072017-151034/.

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A infecção por Actinobacillus pleuropneumoniae, doença conhecida como pleuropneumonia suína, assumiu grande importância na suinocultura moderna devido à alta ocorrência observada nos rebanhos. O impacto da doença está relacionado à capacidade do agente em causar pneumonia severa, levando os animais a óbito ou doença crônica, resultando em graves prejuízos zootécnicos. Diante desse cenário, o controle e monitoramento do agente se faz importante por meio da identificação dos diferentes sorotipos, da análise genética e da determinação dos perfis de resistência aos antimicrobianos. O objetivo do presente estudo foi caracterizar fenotípica e genotípicamente estirpes de Actinobacillus pleuropneumoniae isoladas a partir de quadros de pneumonia em suínos. Um total de 85 estipes de A. pleuropneumoniae foram submetidas a reação em cadeia pela polimerase (PCR) para identificação e sorotipagem, determinação da concentração inibitória mínima de antimicrobianos, polimorfismo do comprimento de fragmentos amplificados (AFLP) e eletroforese em gel de campo pulsado (PFGE). Os sorotipos mais frequentes foram: 5 (38,8%), 10 (29,4%), 7 (5,9%), 8 (5,9%) e 6 (3,5%), sendo que 14 (16,5%) estirpes foram não tipáveis. Foi observada alta heterogeneidade de perfil genético entre as estirpes analisadas, tanto pelo AFLP quanto pelo PFGE, e o índice discriminatório para cada técnica foi 0,97 e 0,84, respectivamente. Todas as estirpes foram sensíveis ao ceftiofur, gentamicina, tulatromicina e tilmicosina, sendo que 98,8% das estirpes foram resistentes à tilosina e altas taxas de resistência foram observadas ainda para as tetraciclinas, clindamicina e sulfadimetoxina.
Infection by Actinobacillus pleuropneumoniae, a disease known as swine pleuropneumonia, has gained greater relevance to modern pig farming due to the high recurrence rate observed in herds. The impact of the disease relates to the capacity of the agent to cause severe pneumonia, leading to animal death or chronic conditions, thus resulting in severe zootechnical losses. In view thereof, the control and monitoring of the agent is key, being performed through the identification of different serotypes, genetic analysis and determination of antimicrobial resistance profiles. The objective of this study was to characterize phenotypically and genotypically Actinobacillus pleuropneumoniae strains isolated from swine with clinical presentation of pneumonia. A total of 85 strains of A. pleuropneumoniae were subject to polymerase chain reaction (PCR) for identification and serotyping, determination of the minimal inhibitory concentration, amplified fragment length polymorphism (AFLP) and pulsed field gel electrophoresis typing techniques (PFGE). Most recurring serotypes were: 5 (38.8%), 10 (29.4%), 7 (5.9%), 8 (5.9%) and 6 (3.5%), of which 14 (16.5%) strains were nontypeable. High genetic heterogeneity was observed for both AFLP and PFGE, and the discriminatory index for each technique was 0.97 and 0.84, respectively. All 85 strains were susceptible to ceftiofur, gentamicin, tulatromicin and tilmicosin, 84 of which were resistant to tylosin, and high resistance rates were also observed for clindamycin, tetracyclines and sulfadimethoxine.
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Book chapters on the topic "Actinobacillus pleuropneumoniae"

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Frey, J. "Exotoxins of Actinobacillus Pleuropneumoniae." In Haemophilus, Actinobacillus, and Pasteurella, 101–13. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4899-0978-7_9.

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Frey, Joachim. "RTX-toxins in Actinobacillus pleuropneumoniae and their potential role in virulence." In Developments in Plant Pathology, 325–40. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0746-4_23.

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Nahar, Nusrat, Conny Turni, Greg Tram, Patrick J. Blackall, and John M. Atack. "Actinobacillus pleuropneumoniae: The molecular determinants of virulence and pathogenesis." In Advances in Microbial Physiology, 179–216. Elsevier, 2021. http://dx.doi.org/10.1016/bs.ampbs.2020.12.001.

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Conference papers on the topic "Actinobacillus pleuropneumoniae"

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Mul, Monique, P. Becker, C. van der Peet-Schwering, and N. Stockhofe-Zurwieden. "Garlic reduces effect of Actinobacillus pleuropneumoniae infection in pigs." In Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2011. http://dx.doi.org/10.31274/safepork-180809-600.

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Li, Ying, Sanjie Cao, Yiping Wen, and Xintian Wen. "Complete nucleotide sequence analysis of tolC gene from actinobacillus pleuropneumoniae." In 2014 7th International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2014. http://dx.doi.org/10.1109/bmei.2014.7002882.

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Li, Ren-Feng, Xue-Bin Li, Kun Zhao, and San-Hu Wang. "Web-Based Genomic-Scale Metabolic Pathways Comparative of Two Actinobacillus pleuropneumoniae Strains." In 2010 2nd International Workshop on Database Technology and Applications (DBTA). IEEE, 2010. http://dx.doi.org/10.1109/dbta.2010.5659079.

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Li, Ren-Feng, Xiang-Qin Tian, Jin-Qing Jiang, Xue-Bin Li, and San-Hu Wang. "Notice of Retraction: Genomic Islands Analysis of Three Actinobacillus pleuropneumoniae Strains by Comparative Genomic Methods." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5779990.

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Kruse, A. B., L. R. Nielsen, and L. Alban. "Vaccination against Actinobacillus pleuropneumoniae as an alternative strategy to antimicrobial use in Danish pig herds." In Safe Pork 2015: Epidemiology and control of hazards in pork production chain. Iowa State University, Digital Press, 2015. http://dx.doi.org/10.31274/safepork-180809-331.

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Cişmileanu, Ana, Cornelia Sima, and Constantin Grigoriu. "Quantum dot-based western blot for sensitive detection of pig serum antibody to actinobacillus pleuropneumoniae." In SPIE Proceedings, edited by Valentin I. Vlad. SPIE, 2007. http://dx.doi.org/10.1117/12.756829.

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Dreyfus, A., P. Kuhnert, and J. Frey. "Use of recombinant ApxIV in serodiagnosis of Actinobacillus pleuropneumoniae infections and development of an ApxIV ELISA." In Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-532.

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