Dissertations / Theses on the topic 'Acid-sensitive'
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Jones, John I. W. "Characterisation of acid-sensitive dyspepsia." Thesis, Nottingham Trent University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272811.
Gallovic, Matthew D. "Acid-Sensitive Polymer Microparticles for Subunit Vaccine Delivery." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1468803443.
Lamb, Justin. "Molecular aspects of amino acid sensitive cell cycle control." Thesis, University of Aberdeen, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245976.
Agius, Ronald. "PH-sensitive binding of nickel(II) ions to aspartic acid." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=971555737.
Roberge, Stéphane. "Conjugated Linoleic Acid/Styrene/Butyl Acrylate Bulk and Emulsion Polymerization." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/34536.
Virdee, Susan. "The use of acid sensitive dyes to monitor acid generation and diffusion in thin polymer films." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32562.pdf.
Goss, Heather Vanessa. "Contrasting Chemical Response to Experimental Acidification of Fice Acid-sensitive Streams." Fogler Library, University of Maine, 2006. http://www.library.umaine.edu/theses/pdf/GossHV2006.pdf.
Jadhav, Amol. "Paramagnetic microparticle manipulation for rapid and sensitive nucleic acid sequence identification." Thesis, University of Newcastle upon Tyne, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608361.
Evans, S. W. "The rate of release of aluminium from acidic and acid sensitive soils." Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308840.
Schweitzer, Lawrence D. (Lawrence David). "Identification of novel proteins that regulate the amino acid-sensitive mTORC1 pathway." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/103249.
Cataloged from PDF version of thesis. "February 2016."
Includes bibliographical references.
mTOR is a serine-threonine kinase that, as the catalytic subunit of mTORC1, promotes growth and anabolism. Due to its central role in metabolism, the local and systemic environment surrounding the cell tightly regulate mTORC1. Growth factors and nutrients are each required to activate mTORC1 and promote growth. Activation of mTORC1 by growth factors has been well-elucidated, but it is only recently becoming clear how nutrients, specifically amino acids, activate mTORC1. The presence of amino acids leads to the recruitment of mTORC1 from the cytosol to the surface of the lysosomal membrane, allowing it to be activated downstream of growth factors. This amino acid-induced translocation is mediated by the Rag GTPases and Ragulator (the scaffold for the Rag GTPases and mTORC1 on the lysosomal membrane). Here we describe the identification of two new components of Ragulator, HBXIP and c7orf59, that are required for the lysosomal localization of both the Rag GTPases and mTORC1 and that allowed us to identify new functions that Ragulator fulfills. We also characterized RagA-null mice and RagA-null mouse embryonic fibroblasts (MEFs). RagA is required for embryonic development, and, surprisingly, its deletion in adult mice leads to an expansion of monocytes. MEFs derived from RagA-null embryos display atypical, nutrient-insensitive mTORC1 activation. Finally, we identified c17orf59, a new Ragulator-interacting protein that inhibits the interaction between the Rag GTPases and Ragulator, inhibiting mTORC1 activation by amino acids. We report here our progress in characterizing the components of the amino acid-sensitive mTORC1 pathway and their physiological roles and we discuss the many open questions that remain to be studied regarding how amino acid sufficiency promotes the lysosomal localization of mTORC1.
by Lawrence D. Schweitzer.
Ph. D.
Lowry, Eleanor J. "14-3-3 mediated forward transport and heterodimerisation of acid-sensitive K2p channels." Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.532256.
Alshaikh, Sana. "A novel and sensitive molecular method for nucleic acid discovery in CSF samples." Thesis, University of Manchester, 2011. https://www.research.manchester.ac.uk/portal/en/theses/a-novel-and-sensitive-molecular-method-for-nucleic-acid-discovery-in-csf-samples(65c2353b-40c2-4856-8af6-c64d86773e3e).html.
Bradbeer, Jennifer. "Self-structuring foods based on acid-sensitive gellan gum systems to impact on satiety." Thesis, University of Birmingham, 2014. http://etheses.bham.ac.uk//id/eprint/5169/.
Yaworsky, Karen Lynne. "Engagement of the insulin-sensitive pathway in the stimulation of glucose transport by Ã-lipoic acid." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0020/MQ45881.pdf.
He, Hongyan. "Multifunctional medical devices based on PH-sensitive hydrogels for controlled drug delivery." The Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=osu1148318906.
Löhrer, Daniel Verfasser], Dominik [Akademischer Betreuer] Wiemuth, and Marc [Akademischer Betreuer] [Spehr. "Proteinbiochemische und elektrophysiologische Charakterisierung des bile acid-sensitive ion channels BASIC / Daniel Löhrer ; Dominik Wiemuth, Marc Spehr." Aachen : Universitätsbibliothek der RWTH Aachen, 2017. http://d-nb.info/1162499036/34.
Löhrer, Daniel [Verfasser], Dominik Akademischer Betreuer] Wiemuth, and Marc [Akademischer Betreuer] [Spehr. "Proteinbiochemische und elektrophysiologische Charakterisierung des bile acid-sensitive ion channels BASIC / Daniel Löhrer ; Dominik Wiemuth, Marc Spehr." Aachen : Universitätsbibliothek der RWTH Aachen, 2017. http://d-nb.info/1162499036/34.
Hanafi-Bagby, Dalia. "Towards a fibre optic nucleic acid biosensor, thiazole orange derivatives as sensitive fluorescent probes to detect DNA hybridization." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0016/MQ45848.pdf.
Jakobsson, Emma. "Structural Studies of Echinococcus granulosus Fatty-acid-binding Protein 1 and Human Semicarbazide-sensitive Amine Oxidase." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis: Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5884.
Donnelly, Eilish Teresa. "An investigation of DNA repair in wild-type, amino acid auxotrophs and UV-sensitive mutants of Aspergillus nidulans." Thesis, University of Ulster, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243733.
Klein, Philipp Michael [Verfasser], and Ernst [Akademischer Betreuer] Wagner. "Redox-sensitive and receptor-targeted sequence-defined, cationic carriers for nucleic acid delivery / Philipp Klein ; Betreuer: Ernst Wagner." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1139977741/34.
Klein, Philipp [Verfasser], and Ernst [Akademischer Betreuer] Wagner. "Redox-sensitive and receptor-targeted sequence-defined, cationic carriers for nucleic acid delivery / Philipp Klein ; Betreuer: Ernst Wagner." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1139977741/34.
Helliwell, R. C. "Biogeochemical modelling of acid sensitive systems in Scotland : influence of scale and the potential role of enhanced nitrogen deposition." Thesis, University of Aberdeen, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.592556.
Renshaw, K. J. "Semi-natural vegetation characteristics and the prediction of hydrological and hydrochemical information within moorland, acid-sensitive catchments in upland Wales." Thesis, Swansea University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.638645.
Reinicker, Aaron D. "High Throughput Study of the Structure Sensitive Decomposition of Tartaric and Aspartic Acid on Surfaces Vicinal to Cu(111) and Cu(100)." Research Showcase @ CMU, 2015. http://repository.cmu.edu/dissertations/572.
Gagkas, Zisis. "Effects of broadleaf woodland cover on streamwater chemistry and risk assessments of streamwater acidification in acid-sensitive catchments in the UK." Thesis, University of Edinburgh, 2007. http://hdl.handle.net/1842/2431.
Horn, Christopher D. "Investigation of the effects of thermal enrichment and acid mine drainage on sensitive aquatic biota in the Stony River, Grant County, WV." Morgantown, W. Va. : [West Virginia University Libraries], 2005. https://etd.wvu.edu/etd/controller.jsp?moduleName=documentdata&jsp%5FetdId=3948.
Title from document title page. Document formatted into pages; contains vii, 101 p. : ill. (some col.), maps (part col.). Vita. Includes abstract. Includes bibliographical references.
Evans, Kate Florella. "The effects of acidosis, glutamine starvation and inhibition of the pH sensitive SNAT 2 amino acid transporter on protein metabolism in L6 muscle cells." Thesis, University of Leicester, 2009. http://hdl.handle.net/2381/7335.
Hu, Kang Dickson J. M. "Development and characterization of poly(vinylidene fluoride)-poly(acrylic acid) pore-filledpH-sensitive membranes and potential application on controlled drug release for ruminant animals." *McMaster only, 2007.
Phillipson, Mia. "Acid transport through gastric mucus : A study in vivo in rats and mice." Doctoral thesis, Uppsala University, Department of Medical Cell Biology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3368.
The gastric mucosa is frequently exposed to endogenously secreted hydrochloric acid of high acidity. Gastric mucosal defense mechanisms are arranged at different levels of the gastric mucosa and must work in unison to maintain its integrity.
In this thesis, several mechanisms underlying gastric mucosal resistance to strong acid were investigated in anesthetized rats and mice. The main findings were as follows:
Only when acid secretion occurred did the pH gradient in the mucus gel withstand back-diffusion of luminal acid (100 mM or 155 mM HCl), and keep the juxtamucosal pH (pHjm) neutral. Thus, when no acid secretion occurred and the luminal pH was 0.8-1, the pH gradient was destroyed.
Bicarbonate ions, produced concomitant with hydrogen ions in the parietal cells during acid secretion and blood-borne to the surface epithelium, were carried transepithelially through a DIDS-sensitive transport.
Prostaglandin-dependent bicarbonate secretion seemed to be less important in maintaining a neutral pHjm.
Removal of the loosely adherent mucus layer did not influence the maintenance of the pHjm. Hence, only the firmly adherent mucus gel layer, approximately 80µm thick, seemed to be important for the pHjm.
Staining of the mucus gel with a pH-sensitive dye revealed that secreted acid penetrated the mucus gel from the crypt openings toward the gastric lumen only in restricted paths (channels). One crypt opening was attached to one channel, and the channel was irreversibly formed during acid secretion.
Gastric mucosal blood flow increased on application of strong luminal acid (155 mM HCl). This acid-induced hyperemia involved the inducible but not the neural isoform of nitric oxide synthase. These results suggest a novel role for iNOS in gastric mucosal protection and indicate that iNOS is constitutively expressed in the gastric mucosa.
It is concluded that a pH gradient in the gastric mucus gel can be maintained during ongoing acid secretion, since the acid penetrates the mucus only in restricted channels and bicarbonate is carried from the blood to the lumen via a DIDS-sensitive transporter.
Green, P., Ngozi G. Anyakoha, I. Gispan-Herman, G. Yadid, and Anna Nicolaou. "Arachidonic acid-containing phosphatidylcholine species are increased in selected brain regions of a depressive animal model: implications for pathophysiology." Elsevier, 2009. http://hdl.handle.net/10454/4584.
The Flinders Sensitive Line (FSL) rat is a genetic animal model of depression. Following recent findings that the brain fatty acid composition of FSL is characterised by increased arachidonic acid (AA), we used electrospray tandem mass spectrometry and 1H-NMR to examine lipid species in different brain areas. Cholesterol and sphingolipids were increased in the hypothalamus of the FSL rats. Furthermore, arachidonic acid-containing phosphatidylcholine species (AA-PC) were elevated with PC16:0/20:4, PC18:1/20:4 and PC18:0/20:4 (p<0.003) increased in the hypothalamus and striatum. In contrast, there was a decrease in some docosahexaenoic acid (DHA)-containing species, specifically PC18:1/22:6 (p<0.003) in the striatum and PE18:1/22:6 (p<0.004) in the prefrontal cortex. Since no significant differences were observed in the erythrocyte fatty acid concentrations, dietary or environmental causes for these observations are unlikely. The increase in AA-PC species which in this animal model may be associated with altered neuropathy target esterase activity, an enzyme involved in membrane PC homeostasis, may contribute to the depressive phenotype of the FSL rats.
Roberts, Terri Patricia. "Developmental failure in cochlear hair cells from mouse models of Usher syndrome and the identification of an acid sensitive ionic current in Inner and Outer hair cells." Thesis, University of Sussex, 2013. http://sro.sussex.ac.uk/id/eprint/46460/.
Smit, Michiel Johannes. "Development and validation of selective and sensitive LC-MS/MS Methods for determination of para-aminosalicyclic acid and cycloserine/terizidone applicable to clinical studies for the treatment of tuberculosis." Master's thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29814.
VERMA, SUJIT KUMAR [Verfasser], Sebastian [Akademischer Betreuer] Springer, Sebastian [Gutachter] Springer, Michael [Gutachter] Köhler, Mathias [Gutachter] Winterhalter, and Werner M. [Gutachter] Nau. "Polyelectrolyte Microcapsules: A versatile and sensitive tool for the detection of protein and nucleic-acid analytes / SUJIT KUMAR VERMA ; Gutachter: SEBASTIAN SPRINGER, MICHAEL KÖHLER, MATHIAS WINTERHALTER, WERNER M. NAU ; Betreuer: SEBASTIAN SPRINGER." Bremen : IRC-Library, Information Resource Center der Jacobs University Bremen, 2017. http://d-nb.info/114810397X/34.
Chen, Xiangke. "Vibrational Sum Frequency Generation Studies of Biological and Atmospheric Relevant Interfaces: Lipids, Organosulfur Species and Interfacial Water Structure." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1282061999.
Sequeira, Reynold. "Sustainable Production Strategies for Environmentally Sensitive Industries." University of Cincinnati / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1288976134.
Adebayo, Olajumoke O. "Evaluation of bacterial polymers as protective agents for sensitive probiotic bacteria." Thesis, University of Wolverhampton, 2018. http://hdl.handle.net/2436/621096.
Shah, Bhavik P. "Targeting Fat-Sensitive Pathways In Enteroendocrine Cells Using Nanoparticle-Mediated Drug Delivery." DigitalCommons@USU, 2009. https://digitalcommons.usu.edu/etd/432.
Turelli, Lorenzo. "Étude de nouveaux motifs d'espaceurs dans la construction de conjugués anticorps-médicaments." Electronic Thesis or Diss., Strasbourg, 2023. http://www.theses.fr/2023STRAF063.
The class of Antibody Drug Conjugates (ADCs) represents one of the most fast-growing treatments in oncology therapeutics, enabling the use of highly cytotoxic compounds covalently linked to a monoclonal antibody (mAb) which can selectively get attached to an antigen expressed on the surface of the cancer cell, assuring the precision of this therapy. The linker motif, connecting the mAb to the drug, is key to dictate the modality of the drug’s release as for the pharmacokinetic and pharmacodynamic of the whole conjugate. However, the development of a novel linker is a costly and time-consuming process, in which the ideal motif has to assure both the stability in blood circulation and a fast kinetic in the release of the warhead inside the cancer cell. The work here described aims at addressing the limitations present in this field in two different ways: first by introducing a novel acid sensitive cleavable linker with excellent extracellular stability and selective cleavability in the cancer cell; secondly, embracing a linker-less approach, by introducing a new format of ADC, coined Self Drugged Antibody (SDA), in which the drug is built on the mAb through a Ugi multicomponent reaction
Nicolini, Ariana Marie, and Ariana Marie Nicolini. "Single-Step, Optical Biosensors for the Rapid and Sensitive Detection of Bacterial and Viral Pathogens." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/623158.
DURANTI, GUGLIELMO. "Oxidative stress resistance in skeletal muscle cells: role of vitamin C and redox sensitive transcription factors NF-KB and AP-1." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2005. http://hdl.handle.net/2108/48.
During physical exercise skeletal muscle cells are continuosly exposed to oxidative stress. Thus, they compensate environmental challenges by increasing adaptive responses, characterized by AP-1- and NF-kB-mediated transcriptional up-regulation of endogenous enzymatic and non-enzymatic antioxidants. Also exogenous antioxidants, such as vitamin C contribute to cellular defences. The skeletal muscle cells content of vitamin C is determined both by the efficiency of transport systems and by the ability to maintain the vitamin in its reduced form. Aim of this thesis was to study, in skeletal muscle cells: i) specific antioxidants systems, including glutathione (GSH), thioredoxin reductase (TxR) and antioxidants enzymes; ii) the role of redox sensitive transcription factors, such as AP-1 and NF-kB; iii) the transport of vitamin C, both in the reduced (ascorbic acid, AA) and oxidized form (dehydroascorbic acid, DHA); iiii) the enzymatic systems (NADH-, NADPH-, GSH-, lipoic acid-dependent) involved in vitamin C recycling. To this end, two skeletal muscle cell lines were used (mouse C2C12 and rat L6C5 cells), in both proliferating and differentiated conditions. We found that muscle cell differentiation was associated with increase in antioxidant ability, and this phenomenon was more evident in the L6C5 cell line. C2C12 myoblasts and myotubes show high levels of NF-kB and thioredoxin reductase, together with AP-1 inhibitory complexes. Furthermore, C2C12 cells have antioxidant enzymes highly active thus allowing survival after exposure to oxidative insults. On the contrary, L6C5 myoblasts show a sensitive phenotype, correlated to low levels of thioredoxin reductase, catalase and NF-kB activity, together with high levels of oxidized glutathione (GSSG) and activating AP-1 complexes. Interestingly, this cell line acquires an apoptosis-resistant phenotype, accompanied by drastic changes in the oxidant/antioxidant balance, when induced to differentiate. Indeed, L6C5 myotubes enhance catalase and NF-kB activities and shift AP-1 complexes from an activating to an inhibitory behaviour. Concerning the experiments on vitamin C metabolism, our data show that both cell lines import AA by the SVCT2 transporter, while DHA transport is mediated by glucose carriers GLUT1 and GLUT3. L6C5 myoblasts are more efficient in ascorbic acid transport, while C2C12 cells are more efficient in dehydroascorbic acid transport and ascorbyl free radical/dehydroascorbic acid reduction. Oxidative stress, induced by glutathione depletion, increased SVCT2 expression and thioredoxin reductase-mediated dehydroascorbic acid reduction, especially in differentiated cells. From these data, SVCT2 and NADPH-thioredoxin dependent DHA reduction appear to belong to an inducible system activated in response to oxidative injury.
Liss, Petronella Francina. "Cloning of the gfp (green fluorescent protein) gene downstream of the ldh promoter in a bacteriocin-sensitive strain of Lactobacillus sakei to serve as a reporter strain in bacteriocin studies." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53330.
ENGLISH ABSTRACT: Lactobacillus plantarum 285, isolated from sorghum beer, produces bacteriocin 285, which displays activity against several food spoilage organisms. For future application of bacteriocin 285 in the food industry, it was important to characterize the peptide and identify the genes encoding its production. The effect of bacteriocin 285 on sensitive cells was determined through the use of an indicator (sensitive) organism, Lactobacillus sakei DSM 20017. The indicator strain was genetically modified to express GFP (green fluorescent protein), with the aim of quantifying the antibacterial activity of bacteriocin 285 as a function of GFP fluorescence. Bacteriocin 285 proved to be identical to plantaricin 423 produced by L. plantarum 423. Plantaricin 423 is a class lIa bacteriocin and displays antimicrobial activity towards a broad spectrum of bacteria, including several food spoilage organisms. The sensitivity of L. sakei DSM 20017 towards antibacterial peptides produced by Lactobacillus curvatus DF38, L. plantarum 285, Lactobacillus casei LHS and Lactobacillus salivarius 241 is not limited to the growth stage of the organism. Cells remained sensitive to all four of these bacteriocins, from lag phase to late exponential growth. To inhibit growth of up to 90% of the cells of L. sakei DSM 20017, 1 AU/ml bacteriocin 285 (7 ng/ml) of partially purified bacteriocin 285 was required. However, to kill all viable cells of L. sakei DSM 20017, 16 AU/ml (110 ng/ml) of partially purified bacteriocin 285 was required. The gfpuv gene, encoding GFPuv, was cloned downstream of the Idh promoter and successfully expressed in L. sakei DSM 20017. However, GFPuv fluorescence could not be used as a direct method to quantify the antimicrobial activity of bacteriocin 285, since cells of strain DSM 20017 remained fluorescent for prolonged periods after treatment with lethal concentrations of the bacteriocin. The non-viability of the cells was confirmed with epifluorescence microscopy and a L1VE/DEAD® Baclight™ Bacterial Viability Probe. Cells that were stained with the viability probe indicated that the majority of untreated L. sakei DSM 20017 cells were viable. However, treatment of strain DSM 20017 with 16 AU/ml bacteriocin 285 rendered all visible cells non-viable.
AFRIKAANSE OPSOMMING: Lactobacillus plantarum 285 wat uit sorgumbier geïsoleer is, produseer bakteriosien 285. Die bakteriosien toon aktiwiteit teen verskeie organismes wat voedselbederi veroorsaak. Vir toekomstige aanwending van bakteriosien 285 in die voedselindustrie was dit belangrik om die peptied te karakteriseer en die gene wat vir die produksie daarvan kodeer, te identifiseer. Die effek van bakteriosien 285 op sensitiewe selle is bepaal deur die gebruik van 'n indikator (sensitiewe)-organisme, Lactobacillus sakei DSM 20017. Die indikator-organisme is geneties verander om die GFP (groen fluoreserende proteïen) uit te druk. Die doel was om die antibakteriese aktiwiteit van bakteriosien 285 te kwantifiseer as 'n funksie van GFP fluorisensie. Bakteriosien 285 is identies aan plantarisien 423 wat deur L. plantarum 423 produseer word. Plantarisien 423 is 'n klas Iia bakteriosien en vertoon antimikrobiese aktiwiteit teenoor 'n wye verskeidenheid bakterieë, insluitende verskeie organismes wat voedsel bederf. Die sensitiwiteit van L. sakei DSM 20017 teenoor antibakteriese peptiede wat deur Lactobacillus cutveius DF38, L. plantarum 285, Lactobacillus casei LHS en Lactobacillus salivarius 241 geproduseer word, word nie beïnvloed deur die groeifase van die organisme nie. Selle het sensitief gebly teenoor al vier die bakteriosiene van sloer- tot laat eksponensiële groei. Om groei van tot 90% van L. sakei DSM 20017 selle te inhibeer, word 1 AU/ml (7 ng/ml) gedeeltelik gesuiwerde bakteriosien 285 benodig. Om alle lewensvatbare L. sakei DSM 20017 selle te dood, word 16 AU/ml (110 ng/ml) gedeeltelik gesuiwerde bakteriosien 285 benodig. Die gfpuv-geen, wat GFPuv kodeer is stroomaf van die Idh-promoter gekloneer en suksesvol in L. sakei DSM 20017 uitgedruk. GFPuv fluoresensie kon nie as direkte metode gebruik word om die antimikrobiese aktiwiteit van bakteriosien 285 te bepaal nie, aangesien die selle van L. sakei DSM 20017 fluoreserend gebly het lank na behandeling met dodelike konsentrasies van die bakteriosien. Die lewensvatbaarheid van die selle is bevestig deur epifluoresensiemikroskopie en 'n LlVE/DEAD® Bac/ight™ bakteriese lewensvatbaarheidspeiler. Selle van L. sakei DSM 20017 wat deur die peiler gekleur is, het gewys dat die meeste selle wat nie deur bakteriosien 285 behandel was nie, lewensvatbaar was. Behandeling van L. sakei DSM 20017 met 16 AU/ml bakteriosien 285 het al die sigbare selle gedood.
Vendrell, Arasa Alexandre. "SCF cdc4 regulates msn2 and msn4 dependent gene expression to counteract hog1 induced lethality." Doctoral thesis, Universitat Pompeu Fabra, 2009. http://hdl.handle.net/10803/7153.
També hem observat que la mort cel·lular causada per l'activació sostinguda de Hog1 és deguda a una inducció d'apoptosi. L'apoptosi induïda per Hog1 és inhibida per la mutació al complexe SCFCDC4. Per tant, la via d'extensió de la vida és capaç de prevenir l'apoptosi a través d'un mecanisme desconegut.
Sustained Hog1 activation leads to an inhibition of cell growth. In this work, we have observed that the lethal phenotype caused by sustained Hog1 activation is prevented by SCFCDC4 mutants. The prevention of Hog1-induced cell death by SCFCDC4 mutation depends on the lifespan extension pathway. Upon sustained Hog1 activation, SCFCDC4 mutation increases Msn2 and Msn4 dependent gene expression that leads to a PNC1 overexpression and a Sir2 deacetylase hyperactivation. Then, hyperactivation of Sir2 is able to prevent cell death caused by sustained Hog1 activation.
We have also observed that cell death upon sustained Hog1 activation is due to an induction of apoptosis. The apoptosis induced by Hog1 is decreased by SCFCDC4 mutation. Therefore, lifespan extension pathway is able to prevent apoptosis by an unknown mechanism.
Chang, Chia-Yu, and 張家瑜. "Isolation and Characterization of Acid Sensitive Mutants from Streptococcus mutans." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/31736392427705344764.
國立臺灣大學
微生物學研究所
86
Streptococcus mutans is an etiological agent of human dental caries. The bacterium expresses two important virulence traits for cariogenisity; the ability of the bacterium to colonize specifically on the tooth surface and to produce lactic acid resulting in demineralization of enamel surface. In addition, S. mutans can survive in acid environment, where the pH is below 3.0. It is the most acid tolerant species among the oral streptococci. The aim of this research is to identify genes are involved in acid tolerance(aciduricity) controlling of S. mutans to survive under the acidic environment. The strategy was construction a mutant library using a suicide vectors, pVA891. This plasmid was ligated with Sau3AI randomly digested chromosomal DNA fragment from S. mutans GS-5. The resultant plasmid pools were randomly integrated into the chromosome of strain GS-5. Acid sensitive mutants were screened by inability to survive on the acidic (pH5.5) agar plate, and confirmed by monitoring their growth under the pH5.5 condition. Acid sensitive mutants were analyzed for pVA891 integration by southern hybridization. Finally, mutant chromosomal DNA fragments flanking the plasmids were recovered by a marker rescue method in Escherichia coli and the flanking region was sequenced. In this study, we screened 2690 mutants, and one (74(3)-1-1) was an acid sensitive mutant. Interestingly, 74(3)-1-1, can not grow at acidic medium under the oxygen rich condition, but it restores acid tolerant ability under the anaerobic condition. The flanking regions of p74(3)H1, were partially sequenced, but it has no homology with other genes of the identical function.
Goss, Heather Vanessa. "Contrasting chemical response to experimental acidification of five acid-sensitive streams /." 2006. http://www.library.umaine.edu/theses/pdf/GossHV2006.pdf.
Agius, Ronald [Verfasser]. "PH-sensitive binding of nickel(II) ions to aspartic acid / Ronald Agius." 2004. http://d-nb.info/971555737/34.
Chien-Yi, Chang, and 張千益. "Characterization of an acid sensitive mutant of Streptococcus mutans with pVA891 integration." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/98917073399957109634.
國立臺灣大學
微生物學研究所
88
英文摘要 Streptococcus mutans is an etiological agent of human dental caries. The bacterium can metabolize many kinds of sugar and produce acidic products resulting in demineralization of tooth surface. Previous studies indicated that S. mutans can grow in acidic environment and survive below the pH value of 3.0. Acid production and acid tolerance are major virulence factors of S. mutans. Screening acid sensitive mutants with suicide vector pVA891 integration, we have identified strain 74(3)-1-1 which could not grow in pH value of 5.5 under aerobic condition, but could grow under the same acidic condition, if cultured anaerobically. The DNA fragments flanking the integrated plasmid were recovered from 74(3)-1-1 by marker rescue and the nucleotide sequence was determined. Using the rescued fragment as a probe to screen a S. mutans chromosomal library, several positive clones were identified. One of the clones, Zap7, was completely sequenced and contained three opene reading frames, cel, phsp, and tkt. Southern blot analysis confirmed the restriction maps of the genes and found that phsp was duplicated in 74(3)-1-1, and therefore, the integration of pVA891 did not inactivate the phsp gene. By monitoring the growth in bioreactor, we confirmed that 74(3)-1-1 can grow at pH of 5.0 under strictly anaerobic condition, but grow pooly under aerobic condition with oxygen tension of 30%. When tested in an in vitro assay system, 74(3)-1-1 exhibited enhanced ability to adhere to glass surfaces in the presence or absence of sucrose. In addition, 74(3)-1-1 cannot grow in a define medium in which glucose is the sole carbon source.
Sahoo, Anasurya. "PH sensitive fiber derived from copolymer of acrylonitrile and ocrylic acid derivative." Thesis, 2007. http://localhost:8080/xmlui/handle/12345678/3901.
Grell, Mary Ann Elizabeth. "Soil Chemistry Characterization of Acid Sensitive Watersheds in the Great Smoky Mountains National Park." 2010. http://trace.tennessee.edu/utk_gradthes/802.
Lee, Yen Chen, and 李彥臻. "Applications of Thermo-sensitive Hydrogel Containing Gelatin and Hyaluronic Acid in Cartilage Tissue Engineering." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/45489401906653561499.
長庚大學
化工與材料工程學系
98
Thermo-sensitive co-polymers based on poly(N-isopropylacrylamide) and contain hyaluronic acid (HPN) or hyaluronic acid and gelatin (HPN-G) were synthesized and characterized for applications in cartilage tissue engineering. Thermo-responsive characteristics of HPN and HPN-G and the feasibility of those polymers for chondrocytes cultures were studied. From in vitro cell culture experiments, the results showed that HPN-G hydrogel is preferred over HPN for differentiation and proliferation of chondrocytes. HPN-G hydrogel alone or by combining with porous polycaprolactone disk produced by laser sintering (G-P) were then used for in vivo animal studies using nude mice. The real-time PCR and staining results suggested that HPN-G scaffold not only helped chondrocytes maintain normal cell functions, but also supported excretion of type II collagen, SOX9, and aggregan. With its free flowing property at room temperature and reversible gelling property at physiological temperature, HPN-G polymer will be a suitable injectable scaffold for cartilage tissue engineering. By mixing polymer solution with chondrocytes at room temperature and cultured at physiological temperature, the hydrogel copolymer will provide a high cell loading efficiency and a suitable environment for chondrocytes proliferation and differentiation while maintaining high cell viability.