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Journal articles on the topic 'ACAE'

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Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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1

Chiou, Jeng-Feng, Alexander T. H. Wu, Wei-Tin Wang, Tsu-Hsiang Kuo, Juri G. Gelovani, I.-Hsin Lin, Chih-Hsiung Wu, Wen-Ta Chiu, and Win-Ping Deng. "A Preclinical Evaluation ofAntrodia camphorataAlcohol Extracts in the Treatment of Non-Small Cell Lung Cancer Using Non-Invasive Molecular Imaging." Evidence-Based Complementary and Alternative Medicine 2011 (2011): 1–12. http://dx.doi.org/10.1093/ecam/nep228.

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This study was carried out to provide a platform for the pre-clinical evaluation of anti-cancer properties of a unique CAM (complementary and alternative medicine) agent,Antrodia camphorataalcohol extract (ACAE), in a mouse model with the advantageous non-invasivein vivobioluminescence molecular imaging technology.In vitroanalyses on the proliferation, migration/invasion, cell cycle and apoptosis were performed on ACAE-treated non-small cell lung cancer cells, H441GL and control CGL1 cells.In vivo, immune-deficient mice were inoculated subcutaneously with H441GL followed by oral gavages of ACAE. The effect of ACAE on tumor progression was monitored by non-invasive bioluminescence imaging. The proliferation and migration/invasion of H441GL cells were inhibited by ACAE in a dose-dependent manner. In addition, ACAE induced cell cycle arrest at G0/G1 phase and apoptosis in H441GL cells as shown by flow cytometric analysis, Annexin-V immunoflourescence and DNA fragmentation.In vivobioluminescence imaging revealed that tumorigenesis was significantly retarded by oral treatment of ACAE in a dose-dependent fashion. Based on our experimental data, ACAE contains anti-cancer properties and could be considered as a potential CAM agent in future clinical evaluation.

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2

Liu, Hen-Yu, Chiung-Fang Huang, Chun-Hao Li, Ching-Yu Tsai, Wei-Hong Chen, Hong-Jian Wei, Ming-Fu Wang, Yueh-Hsiung Kuo, Mei-Leng Cheong, and Win-Ping Deng. "Osteoporosis Recovery byAntrodia camphorataAlcohol Extracts through Bone Regeneration in SAMP8 Mice." Evidence-Based Complementary and Alternative Medicine 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/2617868.

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Antrodia camphoratahas previously demonstrated the efficacy in treating cancer and anti-inflammation. In this study, we are the first to evaluateAntrodia camphorataalcohol extract (ACAE) for osteoporosis recoveryin vitrowith preosteoblast cells (MC3T3-E1) andin vivowith an osteoporosis mouse model established in our previous studies, ovariectomized senescence accelerated mice (OVX-SAMP8). Our results demonstrated that ACAE treatment was slightly cytotoxic to preosteoblast at 25 μg/mL, by which the osteogenic gene expression (RUNX2, OPN, and OCN) was significantly upregulated with an increased ratio of OPG to RANKL, indicating maintenance of the bone matrix through inhibition of osteoclastic pathway. Additionally, evaluation by Alizarin Red S staining showed increased mineralization in ACAE-treated preosteoblasts. Forin vivostudy, our results indicated that ACAE inhibits bone loss and significantly increases percentage bone volume, trabecular bone number, and bone mineral density in OVX-SAMP8 mice treated with ACAE. Collectively,in vitroandin vivoresults showed that ACAE could promote osteogenesis and prevent bone loss and should be considered an evidence-based complementary and alternative medicine for osteoporosis therapy through the maintenance of bone health.

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Chalumeau, Vanessa, Raphaël Blanc, Hocine Redjem, Gabriele Ciccio, Stanislas Smajda, Jean-Philippe Desilles, Daniele Botta, et al. "Anterior cerebral artery embolism during thrombectomy increases disability and mortality." Journal of NeuroInterventional Surgery 10, no. 11 (May 8, 2018): 1057–62. http://dx.doi.org/10.1136/neurintsurg-2018-013793.

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ObjectiveDuring thrombectomy, thromboembolic migration in previously unaffected territory may occur and is not systematically notified. We report our data on the incidence, predictors, and clinical outcome of anterior cerebral artery emboli (ACAE).MethodsFrom a prospectively collected thrombectomy database of consecutive patients with anterior circulation stroke between January 2012 and December 2016, 690 angiographic images were analyzed to assess ACAE. The primary outcome was a favorable outcome, defined as a 3 month modified Rankin Scale score of 0–2 or equal to the pre-stroke score.ResultsACAE occurred in 65 patients (9.4%; 95% CI 7.2% to 11.6%). Internal carotid artery occlusion (tandem or terminal), Alberta Stroke Program Early CT Score <7, increasing number of passes, and use of stent retriever alone (compared with distal aspiration alone or combined with stent retriever) were found to be independent predictors of ACAE. Compared with patients without ACAE, patients with ACAE had lower rates, with an adjusted OR (95% CI) of 0.48 (0.25 to 0.92; P=0.027) for favorable outcome and 0.49 (0.25 to 0.96; P=0.038) for early neurologic improvement. ACAE was significantly associated with a higher mortality (adjusted OR 1.93; 95% CI 1.03 to 3.61; P=0.039) and intracranial hemorrhagic complications (adjusted OR 2.45; 95% CI 1.33 to 4.47; P=0.004). Despite a successful reperfusion modified Thrombolysis in Cerebral Infarction score of 2b–3 at the end of the procedure, a favorable outcome was reached in 30% of patients with ACAE compared with 52.4% in the other patients (OR 0.39; 95% CI 0.19 to 0.78; P=0.008).ConclusionsProcedural ACAE was not an uncommon condition, and was associated with increased mortality and disability rates, regardless of the success of reperfusion.

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4

Singh, Vaibhav Pratap, and R. U. Khan. "Effect of different nutrient media on radial growth of Alternaria brassicae (Berk.) Sacc. infecting different Brassica host crops." International Journal of Agricultural Invention 3, no. 02 (November 27, 2018): 137–40. http://dx.doi.org/10.46492/ijai/2018.3.2.7.

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Alternaria brassicae (Berk.) Sacc. is the most common and destructive pathogen of a number of Brassica crops belong to family Brassicaceae. It has been reported from all the continent of the world and causes considerable losses in terms of quality and quantity of crop produce. The present investigation was undertaken in vitro, to know the effect of different nutrient media viz., Potato Dextrose Agar (PDA), V-8 Juice Agar (V8JA), Richard’s Agar (RA), Czapeck’s Dox Agar (CDA) and Corn Meal Agar (CMA) on the growth of A. brassicae isolates collected from the different Brassica host crops i.e. Mustard, Cauliflower, Cabbage and Radish. These isolates were designated on the basis of their hosts as Acae M, Acae Cf, Acae Ca and Acae R, respectively. Radial growth of all isolates was observed after seven days of incubation. The result showed a marked variation in radial growth of A. brassicae isolates. However, maximum growths of all isolates were recorded in V-8 Juice Agar (V8JA) followed by Potato Dextrose Agar (PDA) while the minimum growth of pathogen was observed in Corn Meal Agar (CMA).

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5

Chen, Shuo, and Min Wu. "Attention Collaborative Autoencoder for Explicit Recommender Systems." Electronics 9, no. 10 (October 18, 2020): 1716. http://dx.doi.org/10.3390/electronics9101716.

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Recently, various deep learning-based models have been applied in the study of recommender systems. Some researches have combined the classic collaborative filtering method with deep learning frameworks in order to obtain more accurate recommendations. However, these models either add additional features, but still recommend in the original linear manner, or only extract the global latent factors of the rating matrices in a non-linear way without considering some local special relationships. In this paper, we propose a deep learning framework for explicit recommender systems, named Attention Collaborative Autoencoder (ACAE). Based on the denoising autoencoder, our model can extract the global latent factors in a non-linear fashion from the sparse rating matrices. In ACAE, attention units are introduced during back propagation, enabling discovering potential relationships between users and items in the neighborhood, which makes the model obtain better results in the rating prediction tasks. In addition, we propose how to optimize the training process of the model by proposing a new loss function. Experiments on two public datasets demonstrate the effectiveness of ACAE and its outperformance of competitive baselines.

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6

Wang, Kai, Joost van de Weijer, and Luis Herranz. "ACAE-REMIND for online continual learning with compressed feature replay." Pattern Recognition Letters 150 (October 2021): 122–29. http://dx.doi.org/10.1016/j.patrec.2021.06.025.

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7

Kapadia, Z. Z., D. V. Spracklen, S. R. Arnold, D. J. Borman, G. W. Mann, K. J. Pringle, S. A. Monks, et al. "Impacts of aviation fuel sulfur content on climate and human health." Atmospheric Chemistry and Physics Discussions 15, no. 13 (July 10, 2015): 18921–61. http://dx.doi.org/10.5194/acpd-15-18921-2015.

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Abstract. Aviation emissions impact both air quality and climate. Using a coupled tropospheric chemistry-aerosol microphysics model we investigate the effects of varying aviation fuel sulfur content (FSC) on premature mortality from long-term exposure to aviation-sourced PM2.5 (particulate matter with a dry diameter of < 2.5 μm) and on the global radiation budget due to changes in aerosol and tropospheric ozone. We estimate that present-day non-CO2 aviation emissions with a typical FSC of 600 ppm result in 3597 (95 % CI: 1307–5888) annual mortalities globally due to increases in cases of cardiopulmonary disease and lung cancer, resulting from increased surface PM2.5 concentrations. We quantify the global annual mean combined radiative effect (REcomb) of non-CO2 aviation emissions as −13.3 mW m−2; from increases in aerosols (direct radiative effect and cloud albedo effect) and tropospheric ozone. Ultra-low sulfur jet fuel (ULSJ; FSC =15 ppm) has been proposed as an option to reduce the adverse health impacts of aviation-induced PM2.5. We calculate that swapping the global aviation fleet to ULSJ fuel would reduce the global aviation-induced mortality rate by 624 (95 % CI: 227–1021) mortalities a−1 and increase REcomb by +7.0 mW m−2. We explore the impact of varying aviation FSC between 0–6000 ppm. Increasing FSC increases annual mortality, while enhancing climate cooling through increasing the aerosol cloud albedo effect (aCAE). We explore the relationship between the injection altitude of aviation emissions and the resulting climate and air quality impacts. Compared to the standard aviation emissions distribution, releasing aviation emissions at the ground increases global aviation-induced mortality and produces a net warming effect, primarily through a reduced aCAE. Aviation emissions injected at the surface are 5 times less effective at forming cloud condensation nuclei, reducing the aviation-induced aCAE by a factor of 10. Applying high FSCs at aviation cruise altitudes combined with ULSJ fuel at lower altitudes result in reduced aviation-induced mortality and increased negative RE compared to the baseline aviation scenario.

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8

Dall’Acqua, Stefano, Kouadio Ibrahime Sinan, Irene Ferrarese, Stefania Sut, Kouadio Bene, Mohamad Fawzi Mahomoodally, Nabeelah Bibi Sadeer, Gunes Ak, and Gokhan Zengin. "Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts." Molecules 25, no. 23 (November 25, 2020): 5537. http://dx.doi.org/10.3390/molecules25235537.

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Breynia retusa (Dennst.) Alston (also known as Cup Saucer plant) is a food plant with wide applications in traditional medicine, particularly in Ayurveda. Extracts obtained with four solvents (dichloromethane, methanol, ethyl acetate and water), from three plant parts, (fruit, leaf and bark) were obtained. Extracts were tested for total phenolic, flavonoid content and antioxidant activities using a battery of assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), total antioxidant capacity (TAC) (phosphomolybdenum) and metal chelating. Enzyme inhibitory effects were investigated using acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase as target enzymes. Results showed that the methanolic bark extract exhibited significant radical scavenging activity (DPPH: 202.09 ± 0.15; ABTS: 490.12 ± 0.18 mg Trolox equivalent (TE)/g), reducing potential (FRAP: 325.86 ± 4.36: CUPRAC: 661.82 ± 0.40 mg TE/g) and possessed the highest TAC (3.33 ± 0.13 mmol TE/g). The methanolic extracts were subjected to LC-DAD-MSn and NMR analysis. A two-column LC method was developed to separate constituents, allowing to identify and quantify forty-four and fifteen constituents in bark and fruits, respectively. Main compound in bark was epicatechin-3-O-sulphate and isolation of compound was performed to confirm its identity. Bark extract contained catechins, procyanidins, gallic acid derivatives and the sulfur containing spiroketal named breynins. Aerial parts mostly contained flavonoid glycosides. Considering the bioassays, the methanolic bark extract resulted a potent tyrosinase (152.79 ± 0.27 mg kojic acid equivalent/g), α-amylase (0.99 ± 0.01 mmol acarbose equivalent ACAE/g) and α-glucosidase (2.16 ± 0.01 mmol ACAE/g) inhibitor. In conclusion, methanol is able to extract the efficiently the phytoconstituents of B. retusa and the bark is the most valuable source of compounds.

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9

Quintanilha, Denise Penna, and Zenilda Alexandre Pasquini. "TAKEO SAWADA: UMA ANÁLISE DE SUA PRÁTICA DOCENTE NO CURSO “PINTURA INFANTIL COM CRIATIVIDADE” DA ACAE EM PRESIDENTE PRUDENTE." Colloquium Humanarum 6, no. 1 (June 25, 2009): 34–43. http://dx.doi.org/10.5747/ch.2009.v06.n1.h063.

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10

Stenehjem, David D., Irene Santi, Komal Gupte-Singh, John R. Penrod, and Meena Venkatachalam. "Estimated costs of grade 3–4 all-cause and treatment-related adverse events for stage IV or recurrent non-small cell lung cancer (NSCLC) in the CheckMate 227 (CM-227) trial." Journal of Clinical Oncology 37, no. 8_suppl (March 10, 2019): 120. http://dx.doi.org/10.1200/jco.2019.37.8_suppl.120.

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120 Background: In the ongoing phase 3 CM-227 trial, nivolumab + ipilimumab (N+I) showed significantly longer progression-free survival compared to chemotherapy (C) among patients with NSCLC and tumor mutational burden (TMB) ≥10 mutations/megabase (high) by Foundation One CDx. The frequencies of all-cause (AC) and treatment-related (TR) adverse events (AEs) including grade 3–4 AEs were similar between the N+I and C arms, although the types of AEs differed. This study estimated the costs of managing grade 3–4 ACAEs and TRAEs in the first year after treatment initiation in CM-227 patients with high TMB. Methods: The frequency, grade, and attribution of AEs were extracted from CM-227 patient-level data. Costs of managing each grade 3–4 ACAE and grade 3–4 TRAE were estimated using related codes of the International Classification of Disease, 10th Revision, as well as cost information from the Healthcare Cost and Utilization Project National Inpatient Sample data (2015). A sensitivity analysis accounting for treatment duration and a temporal trends analysis were conducted. Results: In patients treated with N+I (n = 135), 157 grade 3–4 ACAEs and 70 grade 3–4 TRAEs were observed compared to 153 and 74 respectively among the patients treated with C (n = 159). The cost of managing grade 3–4 ACAEs per treated patient for N+I was $19,602 and for C was $16,353 (difference $3,249; 95% CI $1,774–$4,723), while the cost of managing grade 3–4 TRAEs per treated patient was $6,921 and $8,925 for N+I and C, respectively (difference $2,004; 95% CI $878–$3,131). An analysis adjusting for treatment duration yielded lower cost per person-month for N+I compared to C for both grade 3–4 ACAEs ($2,886 and $3,521; difference $634; 95% CI $379–$889) and grade 3–4 TRAEs ($1,019 and $1,921, difference $902; 95% CI $696–$1,109). For both N+I and C, the cost of managing grade 3–4 ACAEs and grade 3–4 TRAEs per person-month was highest in the first 3 months and declined thereafter. Conclusions: The cost of ACAEs was somewhat higher for N+I than for C, while the cost of TRAEs was somewhat lower for N+I than for C. Adjusting for treatment duration, N+I had lower costs for both ACAEs and TRAEs compared to C. Clinical trial information: NCT02477826.

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11

Angelini, Paola, Roberto Venanzoni, Giancarlo Angeles Flores, Bruno Tirillini, Giustino Orlando, Lucia Recinella, Annalisa Chiavaroli, et al. "Evaluation of Antioxidant, Antimicrobial and Tyrosinase Inhibitory Activities of Extracts from Tricholosporum goniospermum, an Edible Wild Mushroom." Antibiotics 9, no. 8 (August 13, 2020): 513. http://dx.doi.org/10.3390/antibiotics9080513.

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Tricholosporum goniospermum (Bres.) Guzmán ex T.J. Baroni is an excellent edible mushroom whose compounds and biological properties are still unknown. In this study, n-hexane, ethyl acetate and methanol extracts from fruiting bodies and liquid-cultured mycelia were compared for the analysis of phenolic compounds, the evaluation of scavenger (DPPH, ABTS) and reducing (CUPRAC, FRAP) activities, and the enzyme inhibition of α-amylase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase. Additionally, T. goniospermum extracts were evaluated for antibacterial and antimycotic activities against Gram+ and Gram− bacteria, and clinical yeast and fungal dermatophytes. Finally, based on the extract content in phenolic compounds, in silico studies, including the docking approach, were conducted to predict the putative targets (namely tyrosinase, lanosterol-14-α-demethylase, the multidrug efflux system transporters of E. coli (mdtK) and P. aeruginosa (pmpM), and S. aureus β-lactamase (ORF259)) underlying the observed bio-pharmacological and microbiological effects. The methanolic extract from mycelia was the richest in gallic acid, whereas the ethyl acetate extract from fruiting bodies was the sole extract to show levels of catechin. Specifically, docking runs demonstrated an affinity of catechin towards all docked proteins, in the micromolar range. These in silico data are consistent, at least in part, with the highest activity of ethyl acetate extract as an antimicrobial and anti-tyrosinase (554.30 mg KAE/g for fruiting bodies and 412.81 mg KAE/g for mycelia) agent. The ethyl acetate extracts were also noted as being the most active (2.97 mmol ACAE/g for fruiting bodies and 2.25 mmol ACAE/g for mycelia) on α-amylase. BChE inhibitory activities varied from 2.61 to 26.78 mg GALAE/g, while the tested extracts were not active on AChE. In conclusion, all mushroom extracts tested in this study had potent antimicrobial activities. Particularly, among the tested extracts, the ethyl acetate extract showed the highest efficacy as both an antimicrobial and anti-tyrosinase agent. This could be related, albeit partially, to its content of catechin. In this regard, the bioinformatics analyses showed interactions of catechin with tyrosinase and specific microbial proteins involved in the resistance to chemotherapeutic drugs, thus suggesting innovative pharmacological applications of T. goniospermum extracts.

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Zengin, Gokhan, Dejan Stojković, Mohamad Fawzi Mahomoodally, Bibi Sharmeen Jugreet, Mehmet Yavuz Paksoy, Marija Ivanov, Uroš Gašić, Monica Gallo, and Domenico Montesano. "Comprehensive Biological and Chemical Evaluation of Two Seseli Species (S. gummiferum and S. transcaucasicum)." Antioxidants 10, no. 10 (September 24, 2021): 1510. http://dx.doi.org/10.3390/antiox10101510.

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Seseli L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two Seseli species, S. gummiferum and S. transcaucasicum were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of S. transcaucasicum (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic S. transcaucasicum extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl- and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of S. gummiferum displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both Seseli species showed lower cytotoxicity towards HaCaT cells (IC50: >500 µg/mL), compared to the water extracts (IC50: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against Staphylococcus lugdunensis (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). S. gummiferum methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of S. lugdunensis to HaCaT cells as well. Taken together, this study demonstrated the two Seseli species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.

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13

Zengin, Gokhan, Mohamad Fawzi Mahomoodally, Kouadio Ibrahime Sinan, Gunes Ak, Ouattara Katinan Etienne, Jugreet B. Sharmeen, Luigi Brunetti, et al. "Chemical Composition and Biological Properties of Two Jatropha Species: Different Parts and Different Extraction Methods." Antioxidants 10, no. 5 (May 17, 2021): 792. http://dx.doi.org/10.3390/antiox10050792.

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Jatropha L. species, in particular, J. curcas and J. gossypiifolia, are well known medicinal plants used for treating various diseases. In the present study, leaf and stem bark extracts of J. curcas and J. gossypiifolia obtained by maceration or homogenizer assisted extraction, were investigated for their phytochemical contents and biological potential as antioxidants, enzyme inhibitors and neuromodulators. In this regard, the gene expression of tumor necrosis factor α (TNFα) and brain-derived neurotrophic factor (BDNF) was investigated in hypothalamic HypoE22 cells. Finally, a bioinformatics analysis was carried out with the aim to unravel the putative mechanisms consistent with both metabolomic fingerprints and pharmacological effects. The leaf extracts of J. curcas showed higher total phenolic content (TPC) and total flavonoid content (TFC) than the stem bark extracts (range: 5.79–48.95 mg GAE/g and 1.64–13.99 mg RE/g, respectively), while J. gossypiifolia possessed TPC and TFC in the range of 42.62–62.83 mg GAE/g and 6.97–17.63 mg RE/g, respectively. HPLC-MS/MS analysis revealed that the leaf extracts of both species obtained by homogenizer assisted extraction are richer in phytochemical compounds compared to the stem bark extracts obtained by the same extraction method. In vitro antioxidant potentials were also demonstrated in different assays (DPPH: 6.89–193.93 mg TE/g, ABTS: 20.20–255.39 mg TE/g, CUPRAC: 21.07–333.30 mg TE/g, FRAP: 14.02–168.93 mg TE/g, metal chelating activity: 3.21–17.51 mg EDTAE/g and phosphomolybdenum assay: 1.76–3.55 mmol TE/g). In particular, the leaf extract of J. curcas and the stem bark extract of J. gossypiifolia, both obtained by homogenizer assisted extraction, showed the most potent antioxidant capacity in terms of free radical scavenging and reducing activity, which could be related to their higher TPC and TFC. Furthermore, anti-neurodegenerative (acetylcholinesterase inhibition: 1.12–2.36 mg GALAE/g; butyrylcholinetserase inhibition: 0.50–3.68 mg GALAE/g), anti-hyperpigmentation (tyrosinase inhibition: 38.14–57.59 mg KAE/g) and antidiabetic (amylase inhibition: 0.28–0.62 mmol ACAE/g; glucosidase inhibition: 0.65–0.81 mmol ACAE/g) properties were displayed differentially by the different extracts. Additionally, the extracts were effective in reducing the gene expression of both TNFα and BDNF, which could be partially mediated by phenolic compounds such as naringenin, apigenin and quercetin. Indeed, the scientific data obtained from the present study complement the several other reports highlighting the pharmacological potentials of these two species, thus supporting their uses as therapeutically active plants.

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Riyanto, Julius, and Sogandi. "Investigating the anti-acne potential of endophytic bacterial extracts isolated from Mangifera casturi in indigenous South Borneo, Indonesia." Journal of Agriculture and Applied Biology 1, no. 2 (December 24, 2020): 54–63. http://dx.doi.org/10.11594/jaab.01.02.03.

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Endophytic bacteria are beneficial microorganisms living in the tis-sues system of various parts of a plant, including fruits, leaves, twigs, and roots. The bacteria are stated as forming colonies without caus-ing any damage to the plant. Thus, this study aimed at isolating endo-phytic bacterial from the leaves, barks, and fruits of Kasturi plant (Mangifera casturi), screening its endophytic bacteria, determining the potential of those endophytic bacteria, identifying 16S rRNA and analyzing potential growth of the bacteria. The isolated endophytic bacteria appeared to show potential activity against pathogenic bac-teria Propionibacterium acnes with disc-diffusion methods. Besides, the observations on bacterial activities showed isolate L2, S2 and F4 isolated from leaves, bark and fruits, respectively, as the most potent producers of antibacterial compounds. Technically, those activities were indicated by the formation of clear zones. Molecular identifica-tion was investigated by applying PCR amplification on 16S rRNA gene. Furthermore, the isolate L2 was identified as Enterobacter clo-acae with 99% sequence similarities; however, isolates S2 and F4 were identified as Escherichia coli. Therefore, these findings sug-gested that the identified strains would contribute to any further searches for new sources of anti-acne substances.

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Garcia-Gimeno, M. Adelaida, and Kevin Struhl. "Aca1 and Aca2, ATF/CREB Activators in Saccharomyces cerevisiae, Are Important for Carbon Source Utilization but Not the Response to Stress." Molecular and Cellular Biology 20, no. 12 (June 15, 2000): 4340–49. http://dx.doi.org/10.1128/mcb.20.12.4340-4349.2000.

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ABSTRACT In Saccharomyces cerevisiae, the family of ATF/CREB transcriptional regulators consists of a repressor, Acr1 (Sko1), and two activators, Aca1 and Aca2. The AP-1 factor Gen4 does not activate transcription through ATF/CREB sites in vivo even though it binds these sites in vitro. Unlike ATF/CREB activators in other species, Aca1- and Aca2-dependent transcription is not affected by protein kinase A or by stress, and Aca1 and Aca2 are not required for Hog1-dependent salt induction of transcription through an optimal ATF/CREB site. Aca2 is important for a variety of biological functions including growth on nonoptimal carbon sources, and Aca2-dependent activation is modestly regulated by carbon source. Strains lacking Aca1 are phenotypically normal, but overexpression of Aca1 suppresses some defects associated with the loss of Aca2, indicating a functional overlap between Aca1 and Aca2. Acr1 represses transcription both by recruiting the Cyc8-Tup1 corepressor and by directly competing with Aca1 and Aca2 for target sites. Acr1 does not fully account for osmotic regulation through ATF/CREB sites, and a novel Hog1-dependent activator(s) that is not a bZIP protein is required for ATF/CREB site activation in response to high salt. In addition, Acr1 does not affect a number of phenotypes that arise from loss of Aca2. Thus, members of the S. cerevisiae ATF/CREB family have overlapping, but distinct, biological functions and target genes.

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Placines, Chloé, Viana Castañeda-Loaiza, Maria João Rodrigues, Catarina G. Pereira, Azzurra Stefanucci, Adriano Mollica, Gokhan Zengin, Eulogio J. Llorent-Martínez, Paula C. Castilho, and Luísa Custódio. "Phenolic Profile, Toxicity, Enzyme Inhibition, In Silico Studies, and Antioxidant Properties of Cakile maritima Scop. (Brassicaceae) from Southern Portugal." Plants 9, no. 2 (January 22, 2020): 142. http://dx.doi.org/10.3390/plants9020142.

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Cakile maritima Scop. (sea rocket) is an edible halophyte plant with several ethnomedicinal uses. This work reports the chemical profile and bioactivities of food grade extracts from sea rocket organs. Toxicity was determined on mammalian cells, and phenolic profiling and the quantitation of the main metabolites were made by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Enzymatic inhibition was determined towards acetyl- and butyrylcholinesterase (AChE, BuChE), α-glucosidase, α-amylase, and tyrosinase. Docking studies were performed to tyrosinase, on the major metabolites, and samples were tested for antioxidant properties. Extracts were not toxic, were constituted mainly by flavonoids, and some compounds (roseoside and oleuropein) are here described for the first time in the species. The aerial organs’ ethanol extract had relevant activity towards 2,2-diphenyl-1-picrylhydrazyl [DPPH, half maximal inhibitory concentration (IC50) = 0.59 mg/mL], and ferric-reducing activity power (FRAP, IC50 = 0.99 mg/mL). All samples were more active towards AChE than on BuChE. The ethanol fruits’ extract inhibited α-glucosidase [2.19 mmol of equivalent of acarbose (ACAE)/g]. Samples were active against tyrosinase, especially the aerial organs’ ethanol extracts [25.9 mg of equivalent of kojic acid (KAE)/g]. Quercetin and kaempferol glycosides fit well into the enzymatic pocket of tyrosinase. Our results suggest sea rocket as a candidate to be further explored as a source of bioactive products.

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Rahmati Ishka, Maryam, Elizabeth Brown, Alexa Rosenberg, Shawn Romanowsky, James A. Davis, Won-Gyu Choi, and Jeffrey F. Harper. "Arabidopsis Ca2+-ATPases 1, 2, and 7 in the endoplasmic reticulum contribute to growth and pollen fitness." Plant Physiology 185, no. 4 (January 28, 2021): 1966–85. http://dx.doi.org/10.1093/plphys/kiab021.

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Abstract Generating cellular Ca2+ signals requires coordinated transport activities from both Ca2+ influx and efflux pathways. In Arabidopsis (Arabidopsis thaliana), multiple efflux pathways exist, some of which involve Ca2+-pumps belonging to the Autoinhibited Ca2+-ATPase (ACA) family. Here, we show that ACA1, 2, and 7 localize to the endoplasmic reticulum (ER) and are important for plant growth and pollen fertility. While phenotypes for plants harboring single-gene knockouts (KOs) were weak or undetected, a triple KO of aca1/2/7 displayed a 2.6-fold decrease in pollen transmission efficiency, whereas inheritance through female gametes was normal. The triple KO also resulted in smaller rosettes showing a high frequency of lesions. Both vegetative and reproductive phenotypes were rescued by transgenes encoding either ACA1, 2, or 7, suggesting that all three isoforms are biochemically redundant. Lesions were suppressed by expression of a transgene encoding NahG, an enzyme that degrades salicylic acid (SA). Triple KO mutants showed elevated mRNA expression for two SA-inducible marker genes, Pathogenesis-related1 (PR1) and PR2. The aca1/2/7 lesion phenotype was similar but less severe than SA-dependent lesions associated with a double KO of vacuolar pumps aca4 and 11. Imaging of Ca2+ dynamics triggered by blue light or the pathogen elicitor flg22 revealed that aca1/2/7 mutants display Ca2+ transients with increased magnitudes and durations. Together, these results indicate that ER-localized ACAs play important roles in regulating Ca2+ signals, and that the loss of these pumps results in male fertility and vegetative growth deficiencies.

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Youssef, Fadia S., Munira A. Mamatkhanova, Nilufar Z. Mamadalieva, Gokhan Zengin, Salima F. Aripova, Elham Alshammari, and Mohamed L. Ashour. "Chemical Profiling and Discrimination of Essential Oils from Six Ferula Species Using GC Analyses Coupled with Chemometrics and Evaluation of Their Antioxidant and Enzyme Inhibitory Potential." Antibiotics 9, no. 8 (August 14, 2020): 518. http://dx.doi.org/10.3390/antibiotics9080518.

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The differences in the composition of essential oils obtained from the aerial parts of six Ferula species viz., F. caratavica (Fc), F. kuchistanica (Fk), F. pseudoreoselinum (Fp), F. samarcandica (Fs), F. tenuisecta (Ft) and F. varia (Fv) were detected both qualitatively and semi-quantitatively using GC-MS and GC-FID analyses. One hundred and six metabolites were identified that account for 92.1, 96.43, 87.43, 95.95, 92.90 and 89.48% of Fc, Fk, Fp, Fs, Ft and Fv whole essential oils, respectively. The data from the GC-MS analyses were subjected to unsupervised pattern recognition chemometric analysis utilizing principal component analysis (PCA) to improve the visualization of such differences among the six species. Fk and Ft are very closely related to each other and were gathered together in one cluster. The antioxidant potential was assessed in vitro using different assays including 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), ferric reducing power (FRAP) and phosphomolybdenum (PM) assays. Ft and Fp exhibited the most notable antioxidant properties as evidenced by their pronounced activities in most of the antioxidant assays performed, followed by Fc. Fk showed the most effective tyrosinase inhibitory potential, which was estimated as 119.67 mgKAE/g oil, while Fp exhibited the most potent α-amylase inhibitory potential, which was equivalent to 2.61 mmol ACAE/g oil. Thus, it was concluded that Ferula species could serve as a promising natural antioxidant drug that could be included in different products and spices to alleviate hyperglycemia and used as a natural ingredient in pharmaceutical cosmetics to counteract hyperpigmentation.

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Zhang, Leilei, Gabriele Rocchetti, Gökhan Zengin, Gunes Ak, Fatema R. Saber, Domenico Montesano, and Luigi Lucini. "The UHPLC-QTOF-MS Phenolic Profiling and Activity of Cydonia oblonga Mill. Reveals a Promising Nutraceutical Potential." Foods 10, no. 6 (May 28, 2021): 1230. http://dx.doi.org/10.3390/foods10061230.

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Cydonia oblonga Mill., normally known as the quince fruit, has been widely used in agro-food industries mainly to produce jams and jellies. However, other parts of the plants are still underutilized and not completely assessed for their nutraceutical profile. Therefore, in this work, the polyphenolic profile of C. oblonga was investigated using an untargeted metabolomics approach based on high-resolution mass spectrometry. Several compounds were identified in the different parts of the plants, including flavonoids (i.e., anthocyanins, flavones, flavan-3-ols, and flavonols), phenolic acids (both hydroxycinnamics and hydroxybenzoics), low-molecular-weight phenolics (tyrosol equivalents), lignans, and stilbenes. Overall, C. oblonga leaves showed the highest in vitro antioxidant potential, as revealed by 2,2-difenil-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and cupric ion reducing antioxidant capacity (CUPRAC) assays, being 189.5, 285.6, 158.9, and 348.8 mg Trolox Equivalent/g, respectively. The enzymes acetyl- and butyryl-cholinesterases were both inhibited by the different plant parts of C. oblonga, with stems showing the higher inhibitory potential. Interestingly, the fruit extracts were the only parts inhibiting the α-glucosidase, with a value of 1.36 mmol acarbose equivalents (ACAE)/g. On the other hand, strong tyrosinase inhibition was found for stems and leaves, being 72.11 and 68.32 mg Kojic acid Equivalent/g, respectively. Finally, a high number of significant (0.05 < p < 0.01) correlations were outlined between phenolics (mainly anthocyanins, flava-3-ols, and tyrosol equivalents) and the different biological assays. Taken together, our findings suggest a potential exploitation of C. oblonga leaves and stems for the food, pharmaceutical, and cosmetic industries.

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Bibi Sadeer, Nabeelah, Kouadio Ibrahime Sinan, Zoltán Cziáky, József Jekő, Gokhan Zengin, Rajesh Jeewon, Hassan H. Abdallah, Kannan R. R. Rengasamy, and Mohamad Fawzi Mahomoodally. "Assessment of the Pharmacological Properties and Phytochemical Profile of Bruguiera gymnorhiza (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis." Biomolecules 10, no. 5 (May 7, 2020): 731. http://dx.doi.org/10.3390/biom10050731.

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Bruguiera gymnorhiza (L.) Lam. is claimed to effectively manage a number of ailments including diabetes and associated complications. Nonetheless, no attempt has been made to delineate its pharmacological propensities and phytochemical profile. This study was designed to appraise the antioxidant and enzymatic inhibitory properties relevant to the management of diabetes mellitus, obesity, and neurodegenerative and skin disorders. A combination of colorimetric assays and ultra-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) were applied for the phytochemical screening of leaf, root, twig, and fruit extracts (methanol and ethyl acetate). In vitro antioxidant evaluations were via radical scavenging abilities (DPPH, ABTS), reducing potential (FRAP, CUPRAC), chelating power, and total antioxidant capacity (phosphomolybdenum). Seven key metabolic enzymes (α-amylase, α-glucosidase, tyrosinase, elastase, lipase, AChE, and BChE) were targeted to determine the inhibitory effects. Multivariate and in silico docking analysis were performed on collected data. Methanolic fruit extract yielded the highest total phenolic, tannin, and triterpenoid contents (174.18 ± 4.27 mg GAE/g, 176.24 ± 3.10 mg CE/g, 63.11 ± 3.27 mg OAE/g, respectively); significantly depressed tyrosinase, elastase, and α-amylase activities (155.35 ± 0.29 mg KAE/g, 4.56 ± 0.10 mg CAE/g, 1.00 ± 0.05 mmol ACAE/g, accordingly); and harboured the most potent antioxidant capacities with DPPH, CUPRAC, FRAP (492.62 ± 5.31, 961.46 ± 11.18, 552.49 ± 8.71 mg TE/g, respectively), and phosphomolybdenum (4.17 ± 0.31 mmol TE/g) assays. Multivariate analysis suggested that the type of solvents used influenced the biological activities more compared to plant parts. Docking analysis showed that azelaic acid binds with tyrosinase by Van der Waals and conventional hydrogen bonds. We anticipate that the present study may establish baseline data on this halophyte that could open new avenues for the development of biomedicine.

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Thompson, Richard M., and Robert T. Downs. "Systematic generation of all nonequivalent closest-packed stacking sequences of length N using group theory. Erratum." Acta Crystallographica Section B Structural Science 58, no. 1 (January 24, 2001): 153. http://dx.doi.org/10.1107/s0108768102000927.

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In the paper by Thompson & Downs (2001) the last line in Table 1 is missing. The correct table is reprinted here.The two nonequivalent sequences for N = 4 and their symmetrical equivalentsABAB ABAC ACAC ABCB BABA ACAB BCBC ACBC CACA BABC CBCB BACA BCBA BCAC CACB CABA CBCA CBAB

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22

&NA;. "ACAM-2000." Reactions Weekly &NA;, no. 1313 (August 2010): 7. http://dx.doi.org/10.2165/00128415-201013130-00023.

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Balakrishnan, Vijay Shankar. "Jacques Acar." Lancet Infectious Diseases 20, no. 7 (July 2020): 788. http://dx.doi.org/10.1016/s1473-3099(20)30504-1.

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24

&NA;. "ACCE NEWS." Journal of Clinical Engineering 24, no. 1 (January 1999): 17. http://dx.doi.org/10.1097/00004669-199901000-00006.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 18. http://dx.doi.org/10.1097/00004669-199901000-00007.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 18. http://dx.doi.org/10.1097/00004669-199901000-00008.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 19. http://dx.doi.org/10.1097/00004669-199901000-00009.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 20. http://dx.doi.org/10.1097/00004669-199901000-00010.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 20. http://dx.doi.org/10.1097/00004669-199901000-00011.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 20. http://dx.doi.org/10.1097/00004669-199901000-00012.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 20. http://dx.doi.org/10.1097/00004669-199901000-00013.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 21. http://dx.doi.org/10.1097/00004669-199901000-00014.

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&NA;. "ACCE News." Journal of Clinical Engineering 24, no. 1 (January 1999): 21–22. http://dx.doi.org/10.1097/00004669-199901000-00015.

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&NA;. "ACCE News." Journal of Clinical Engineering 25, no. 2 (March 2000): 90–94. http://dx.doi.org/10.1097/00004669-200025020-00010.

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&NA;. "ACCE News." Journal of Clinical Engineering 25, no. 3 (May 2000): 144–49. http://dx.doi.org/10.1097/00004669-200025030-00009.

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Gieras, Izabella. "ACCE Collaborations." Journal of Clinical Engineering 29, no. 2 (April 2004): 58. http://dx.doi.org/10.1097/00004669-200404000-00008.

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Levin-Epstein, Michael. "ACCE News." Journal of Clinical Engineering 42, no. 1 (2017): 15–18. http://dx.doi.org/10.1097/jce.0000000000000199.

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Levin-Epstein, Michael. "ACCE News." Journal of Clinical Engineering 42, no. 2 (2017): 62–65. http://dx.doi.org/10.1097/jce.0000000000000203.

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Levin-Epstein, Michael. "ACCE News." Journal of Clinical Engineering 42, no. 3 (2017): 110–13. http://dx.doi.org/10.1097/jce.0000000000000220.

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&NA;, &NA;. "ACCE@AAMI." Journal of Clinical Engineering 35, no. 4 (October 2010): 180–81. http://dx.doi.org/10.1097/jce.0b013e3181fb99cc.

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&NA;, &NA;. "ACCE International." Journal of Clinical Engineering 35, no. 4 (October 2010): 185–86. http://dx.doi.org/10.1097/jce.0b013e3181fb9a65.

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Lebatha, P. D., J. J. Spies, and M. H. Buys. "HYACINTH ACEAE." Bothalia 33, no. 1 (September 12, 2003): 135–37. http://dx.doi.org/10.4102/abc.v33i1.440.

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Fazio, Sergio, Dwayne E. Dove, and MacRae F. Linton. "ACAT Inhibition." Arteriosclerosis, Thrombosis, and Vascular Biology 25, no. 1 (January 2005): 7–9. http://dx.doi.org/10.1161/01.atv.0000153086.46231.8c.

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Krämer-Hoppe, Rike. "ACAB = Beleidigung?" JURA - Juristische Ausbildung 40, no. 6 (May 1, 2018): 621–30. http://dx.doi.org/10.1515/jura-2018-0128.

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Khund-Sayeed, Syed, Ximiao He, Timothy Holzberg, Jun Wang, Divya Rajagopal, Shriyash Upadhyay, Stewart R. Durell, et al. "5-Hydroxymethylcytosine in E-box motifs ACAT|GTG and ACAC|GTG increases DNA-binding of the B-HLH transcription factor TCF4." Integrative Biology 8, no. 9 (2016): 936–45. http://dx.doi.org/10.1039/c6ib00079g.

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We designed a novel method to double-strand Agilent microarrays such that 5mC and 5hmC are incorporated on one DNA strand. Using protein binding microarrays we demonstrate the utility of this method in exploring how cytosine modification outside of CG dinucleotide alter the DNA binding of sequence-specific transcription factors.

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Ryan, Daniel E., and Koji Nakanishi. "Methine-selective deuteration of V(acac)3, [Co(acac)2]4, and Al(acac)3." Journal of Labelled Compounds and Radiopharmaceuticals 36, no. 6 (June 1995): 595–98. http://dx.doi.org/10.1002/jlcr.2580360611.

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Catlett, John B. "AAAI and ACAI." Allergy and Asthma Proceedings 14, no. 5 (September 1, 1993): 377–78. http://dx.doi.org/10.2500/108854193778774038.

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Hastjarjo, T. Dicky. "Rancangan Eksperimen Acak." Buletin Psikologi 22, no. 2 (December 1, 2014): 73. http://dx.doi.org/10.22146/bpsi.11455.

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Rancangan eksperimen oleh McGuigan(1987) secara umum diartikan sebagaipenerapan metode ilmiah yang diawalidengan merumuskan permasalahan, dilan-jutkan dengan merumuskan hipotesis,menyeleksi partisipan, menempatkan par-tisipan ke kelompok eksperimen dankontrol, menentukan variabel independendan dependen, mengendalikan variabelluar yang relevan, melakukan analisa sta-tistik, membuat generalisasi dan penje-lasan hipotesis jika terkonfirmasi, sertadiakhiri dengan memprediksi terhadapsituasi baru lewat replikasi.

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Dutta, M. Jan. "ACAES activity report." Journal of Asian Economics 6, no. 4 (December 1995): 611–17. http://dx.doi.org/10.1016/1049-0078(95)90033-0.

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&NA;. "ORBIS & ACCE." Journal of Clinical Engineering 32, no. 3 (July 2007): 100–101. http://dx.doi.org/10.1097/01.jce.0000282642.15083.61.

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