Dissertations / Theses on the topic 'Abscisic acid'
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Ferreira, Gisela. "Reguladores vegetais na superação da dormência, balanço hormonal e degradação de reservas em sementes de Annona diversifolia SAFF. e A. purpurea Moc. & Sessé Ex Dunal (Annonaceae) /." Botucatu, 2011. http://hdl.handle.net/11449/106722.
Full textResumo: As sementes de anonáceas são conhecidas por apresentarem mecanismos de dormência, o que dificulta a perpetuação das espécies e a formação de áreas produtivas para a exploração comercial. Deste modo, os objetivos deste trabalho foram estudar curva de aquisição de água; a germinação de sementes tratadas com GA3 e GA4+7 + Benziladenina; o balanço hormonal e a degradação de reservas em sementes de Annona diversifolia Saff e Annona purpurea Moc & Sessé ex Dunal tratadas com reguladores vegetais para a superação da dormência. Para tanto foram realizados três experimentos. Para a construção da curva de aquisição de água foram utilizadas 4 repetições de 25 sementes que foram mantidas em embebição e pesadas durante 480 horas. O segundo experimento foi constituído pela germinação das sementes tratadas com os reguladores vegetais; o delineamento experimental empregado foi o inteiramente casualizado com 4 repetições de 25 sementes por parcela em esquema fatorial 2 x 7 (reguladores x concentrações). Os tratamentos foram constituídos pelas combinações entre concentrações de GA3 e de GA4+7 + Benziladenina (GA4+7 + BA) x 0, 200, 400, 500, 600, 800 e 1000 mg L-1 i.a.. No terceiro experimento foram quantificados ABA (Ácido abscísico) e GA (Giberelinas), proteínas, açúcares... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Annonaceae seeds have been known by presenting dormancy mechanisms, which makes difficult the perpetuation of species and the formation of roductive areas for commercial exploration. Thus, the present work aimed to evaluate water uptake curve; germination of seeds treated with GA3 and GA4+7 + Benzyl adenine; hormone balance and reserve degradation in Annona diversifolia Saff and Annona purpurea Moc & Sessé ex Dunal seeds subjected to plant growth regulators for dormancy break. Three experiments were carried out. To obtain the water uptake curve, four replicates of 25 seeds were kept in imbibition and weighed for 480h. The second experiment evaluated the germination of seeds treated with plant growth regulators; experimental design was completely randomized, with four replicates of 25 seeds per plot in the 2x7 (plant growth regulators x concentrations) factorial arrangement. Treatments consisted of combinations between concentrations of GA3 and GA4+7 + Benzyl adenine (GA4+7 + BA) with 0, 200, 400, 500, 600, 800 and 1000 mg L-1 a.i.. In the third experiment, ABA (abscisic acid), GA (gibberellins), proteins, total soluble sugars and lipids were quantified in seeds soaked in water, without imbibition and soaked in GA4+7 + BA for 15 days (on the 0th, 2nd, 5th, 10th and 15th days). Based... (Complete abstract click electronic access below)
Tarr, Alun Rhys. "Genetically induced abscisic acid deficiencies." Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335475.
Full textSaito, Shigeki. "Studies on (+)-abscisic acid 8'-hydroxylase, a key enzyme in the catabolism of abscisic acid." Kyoto University, 2005. http://hdl.handle.net/2433/144582.
Full text0048
新制・課程博士
博士(農学)
甲第11801号
農博第1521号
新制||農||916(附属図書館)
学位論文||H17||N4075(農学部図書室)
23541
UT51-2005-F831
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 坂田 完三, 教授 矢﨑 一史, 教授 宮川 恒
学位規則第4条第1項該当
Milborrow, Barry Vaughan Biotechnology & Biomolecular Sciences Faculty of Science UNSW. "Biosynthesis of abscisic acid in plants." Awarded by:University of New South Wales. Biotechnology & Biomolecular Sciences, 2007. http://handle.unsw.edu.au/1959.4/42883.
Full textParry, Andrew David. "Abscisic acid biosynthesis in higher plants." Thesis, Cardiff University, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328480.
Full textFarias, Euménes Tavares de [UNESP]. "Expressão gênica no embrião e no endosperma micropilar de sementes de café (coffea arabica L.) durante a germinação." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/86394.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A germinação de sementes de café (Coffea arabica L.) é lenta e irregular, controlada por eventos que ocorrem, simultaneamente, no embrião e no endosperma. Embora os referidos eventos estejam determinados, ainda são necessários estudos sobre a fisiologia molecular, para auxiliar na avaliação da qualidade fisiológica das sementes durante a germinação. O objetivo do trabalho foi realizar estudos fisiológicos e moleculares durante a germinação de sementes embebidas em água e em ácido abscísico (ABA) na concentração de 1000 μM. Durante o trabalho foi determinado o teor de água, a curva de embebição, a germinação, o crescimento do embrião e a expressão dos genes associados com o crescimento do embrião e com a degradação do endosperma micropilar. Para tanto, embriões e os endospermas micropilares foram isolados para a extração de RNA total e síntese de cDNA. “Primers” específicos foram desenhados para o estudo da expressão gênica em PCR em tempo real. Foi estudada a expressão dos genes actina, ciclina e α-expansina, associados ao crescimento do embrião, e α-galactosidase, β-manosidase e endo-β-mananase, associados à degradação do endosperma micropilar. A curva de embebição apresentou um padrão trifásico. A primeira semente de café germinou com cinco dias de embebição e 50% de germinação ocorreram no décimo dia de embebição. A expressão dos genes associados com o crescimento do embrião, tais como actina, α-expansina e quinase dependente de ciclina, aumentou durante a germinação em água e inibiu parcialmente a expressão destes genes quando tratados com ABA. A expressão de β-manosidase e endo-β-mananase aumentou durante a embebição em água e ABA inibiu completamente a expressão. No entanto, α-galactosidase parece ter a expressão mais constitutiva durante a germinação em água e é menos influenciada por ABA, em comparação com outras enzimas estudadas
Germination of coffee (Coffea arabica L.) seed is slow and uneven. The germination is a net result of events that occur simultaneously in the embryo and endosperm under the control of ABA. The aim of the study was to perform physiological and molecular studies during germination of seeds imbibed in water and 1000 μM abscisic acid (ABA). We studied the expression of the genes ciclin, α-expansin and cyclin-dependent of kinase in the embryo and α-galactosidase, β-mannosidase, endo-β-mannanase in the micropylar endosperm. The first coffee seed germinate at five days of imbibition and 50% germinate at tenth day of imbibition. Coffee embryo grew inside the seed pior radicle protrusion and ABA inhibited the embryo grow as well as radicle protrusion. The expression of the genes associated with the growth of the embryo such as ciclin, α-expansin and cyclin-dependent of kinase increased during germination and ABA partially inhibited the expression of these genes. The expression of β-mannosidase and endo-β-mannanase increased during imbibitions in water and ABA completely inhibited its expression. However, α-galactosidase seems to have a more constitutive expression during germination in water and it is less affected by ABA as compared with other enzymes studied
Farias, Euménes Tavares de 1986. "Expressão gênica no embrião e no endosperma micropilar de sementes de café (coffea arabica L.) durante a germinação /." Botucatu :, 2012. http://hdl.handle.net/11449/86394.
Full textBanca: Claudio Cavariani
Banca: Juliana Pereira Bravo
Resumo: A germinação de sementes de café (Coffea arabica L.) é lenta e irregular, controlada por eventos que ocorrem, simultaneamente, no embrião e no endosperma. Embora os referidos eventos estejam determinados, ainda são necessários estudos sobre a fisiologia molecular, para auxiliar na avaliação da qualidade fisiológica das sementes durante a germinação. O objetivo do trabalho foi realizar estudos fisiológicos e moleculares durante a germinação de sementes embebidas em água e em ácido abscísico (ABA) na concentração de 1000 μM. Durante o trabalho foi determinado o teor de água, a curva de embebição, a germinação, o crescimento do embrião e a expressão dos genes associados com o crescimento do embrião e com a degradação do endosperma micropilar. Para tanto, embriões e os endospermas micropilares foram isolados para a extração de RNA total e síntese de cDNA. "Primers" específicos foram desenhados para o estudo da expressão gênica em PCR em tempo real. Foi estudada a expressão dos genes actina, ciclina e α-expansina, associados ao crescimento do embrião, e α-galactosidase, β-manosidase e endo-β-mananase, associados à degradação do endosperma micropilar. A curva de embebição apresentou um padrão trifásico. A primeira semente de café germinou com cinco dias de embebição e 50% de germinação ocorreram no décimo dia de embebição. A expressão dos genes associados com o crescimento do embrião, tais como actina, α-expansina e quinase dependente de ciclina, aumentou durante a germinação em água e inibiu parcialmente a expressão destes genes quando tratados com ABA. A expressão de β-manosidase e endo-β-mananase aumentou durante a embebição em água e ABA inibiu completamente a expressão. No entanto, α-galactosidase parece ter a expressão mais constitutiva durante a germinação em água e é menos influenciada por ABA, em comparação com outras enzimas estudadas
Abstract: Germination of coffee (Coffea arabica L.) seed is slow and uneven. The germination is a net result of events that occur simultaneously in the embryo and endosperm under the control of ABA. The aim of the study was to perform physiological and molecular studies during germination of seeds imbibed in water and 1000 μM abscisic acid (ABA). We studied the expression of the genes ciclin, α-expansin and cyclin-dependent of kinase in the embryo and α-galactosidase, β-mannosidase, endo-β-mannanase in the micropylar endosperm. The first coffee seed germinate at five days of imbibition and 50% germinate at tenth day of imbibition. Coffee embryo grew inside the seed pior radicle protrusion and ABA inhibited the embryo grow as well as radicle protrusion. The expression of the genes associated with the growth of the embryo such as ciclin, α-expansin and cyclin-dependent of kinase increased during germination and ABA partially inhibited the expression of these genes. The expression of β-mannosidase and endo-β-mannanase increased during imbibitions in water and ABA completely inhibited its expression. However, α-galactosidase seems to have a more constitutive expression during germination in water and it is less affected by ABA as compared with other enzymes studied
Mestre
Shinde, Suhas, Shivakumar Devaiah, and Aruna Kilaru. "Profiling Abscisic Acid-Induced Changes in Fatty Acid Composition in Mosses." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etsu-works/4745.
Full textPeng, Xiaobing Carleton University Dissertation Biology. "The role of abscisic acid and abscisic acid-analogs in inducing desiccation tolerance in microspore-derived embryos of Brassica Napus." Ottawa, 1994.
Find full textSmith, Timothy Robert. "Enantioselective Synthesis and Chemical Genomics of Abscisic Acid." Thesis, University of Warwick, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491505.
Full textRoss, Gavin Stuart. "Abscisic acid and the control of seed development." Thesis, Queen's University Belfast, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356970.
Full textJones, Matthew O. "Abscisic acid biosynthesis in tomato and tobacco roots." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479001.
Full textDuckham, Stephen Craig. "The biochemical basis of abscisic acid deficient mutants." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303129.
Full textPriest, David M. "Investigation of a glucosyltransferase that recognises abscisic acid." Thesis, University of York, 2005. http://etheses.whiterose.ac.uk/14075/.
Full textTodoroki, Yasushi. "STUDIES ON HIGHLY POTENT ANALOGUES OF ABSCISIC ACID." Kyoto University, 1996. http://hdl.handle.net/2433/160864.
Full textKyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第6474号
農博第887号
新制||農||720(附属図書館)
学位論文||H8||N2918(農学部図書室)
UT51-96-F353
京都大学大学院農学研究科食品工学専攻
(主査)教授 大東 肇, 教授 安本 教傳, 教授 岩村 俶
学位規則第4条第1項該当
Griffiths, Allen. "Abscisic acid and the water relations of tomato roots." Thesis, Bangor University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306779.
Full textBass, Peter R. "Purification of an enzyme involved in abscisic acid biosynthesis." Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385939.
Full textGuri, Amir Joseph. "Abscisic acid ameliorates glucose tolerance and obesity-induced inflammation." Diss., Virginia Tech, 2007. http://hdl.handle.net/10919/29433.
Full textPh. D.
Cowan, Ashton Keith. "The metabolism of abscisic acid in higher plant tissues." Thesis, Rhodes University, 1989. http://hdl.handle.net/10962/d1002024.
Full textVasanthi, G. "Hormones and Cuscuta Development Abscisic Acid And Its Conjugates-Endogenous Levels And Metabolism During Growth And Haustoria Formation." Thesis, Indian Institute of Science, 1994. http://hdl.handle.net/2005/102.
Full textMohr, Peter G., and lswan@deakin edu au. "Abscisic acid regulation of plant defence responses during pathogen attack." Deakin University. School of Biological and Chemical Sciences, 2004. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20060927.120049.
Full textLinforth, R. "A genetic approach to the study of abscisic acid biosynthesis." Thesis, University of Nottingham, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378491.
Full textZhang, Yi. "Improving Freezing Tolerance of Wine Grapes with Exogenous Abscisic Acid." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354643490.
Full textInomata, Masahiro. "Studies on the biosynthetic pathway of abscisic acid in fungi." Kyoto University, 2005. http://hdl.handle.net/2433/145008.
Full text0048
新制・課程博士
博士(農学)
甲第11606号
農博第1462号
新制||農||904(附属図書館)
学位論文||H17||N3999(農学部図書室)
23249
UT51-2005-D355
京都大学大学院農学研究科食品生物科学専攻
(主査)教授 大東 肇, 教授 村田 幸作, 教授 坂田 完三
学位規則第4条第1項該当
Augustyn-Gradkowska, E. "Stereocontrolled synthesis of plant growth regulators, abscisic acid and xanthoxin." Thesis, London Metropolitan University, 1985. http://repository.londonmet.ac.uk/3314/.
Full textJiang, Mingyi. "Role and mechanism of abscisic acid in the induction of antioxidant defense in maize leaves." HKBU Institutional Repository, 2002. http://repository.hkbu.edu.hk/etd_ra/415.
Full textTang, Yulin. "Analysis of auxin and abscisic acid signal transduction in Arabidopsis thaliana." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970018517.
Full textLiang, Jiansheng. "Xylem-carried abscisic acid (ABA) in plant responses to soil-drying." HKBU Institutional Repository, 1997. http://repository.hkbu.edu.hk/etd_ra/167.
Full textFan, Rong. "The roles of 1-aminocyclopropane-1-carboxylate synthase isogenes in the flower and fruit development in tomatoes." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/b40203906.
Full textJokhan, Anjeela Devi. "Root-to-shoot communication in Ricinus communis L. plants subjected to drying a part of the root system." Thesis, University of Bristol, 1997. http://hdl.handle.net/1983/4a9159a4-0a32-457c-bf08-61e6745f8a75.
Full textMichler, Charles H. "Effects of light quality on morphology and endogenous abscisic acid during somatic embryogenesis of carrot /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487265143147375.
Full textRichardson, Gaynor Rose-Marie. "Cell-free biosynthesis of abscisic acid (ABA) in extracts of flavedo from Citrus sinensis (L.) osbeck." Thesis, Rhodes University, 1996. http://hdl.handle.net/10962/d1003790.
Full textCho, In-jeong. "Function of abscisic acid in maintenance of maize primary root growth under water deficit." Diss., Columbia, Mo. : University of Missouri-Columbia, 2006. http://hdl.handle.net/10355/4459.
Full textThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file plus two media files. Title from title screen of research.pdf file (viewed on May 1, 2009) Vita. Includes bibliographical references.
Sun, Xin. "Characterization of an auxin- and abscisic acid-inducible reporter gene : Dc3-GUS in reported auxin mutants, and mutant screening based on auxin responsive Dc3-GUS expression /." View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202003%20SUN.
Full textYang, Zhen. "AtERF4 and AtERF7 are involved in the abscisic acid response in arabidopsis." Morgantown, W. Va. : [West Virginia University Libraries], 2005. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4170.
Full textTitle from document title page. Document formatted into pages; contains ix, 80 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 65-80).
Cook, Ritchard Matthew. "Changes in gene expression in response to abscisic acid and environmental stress." Thesis, University of the West of England, Bristol, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293362.
Full textGrundy, Jack. "Control of environmental stress responses by the circadian clock and abscisic acid." Thesis, University of Warwick, 2016. http://wrap.warwick.ac.uk/90280/.
Full textBadrakh, Turmandakh. "Effects of Abscisic Acid (ABA) on Germination Rate of Three Rangeland Species." BYU ScholarsArchive, 2016. https://scholarsarchive.byu.edu/etd/5881.
Full textTonini, Patricia Pinho. "Conexão entre os processos de degradação das resservas de proteinas e carboidratos e o edfeito dos hormonios e açucares em sementes de Sesbania virgata (Cav.) Pers." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317725.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Sementes de Sesbania virgata (Cav.) Pers. acumulam suas reservas de carbono no endosperma na forma de um polissacarídeo de parede celular, o galactomanano. A mobilização deste ocorre após a germinação e envolve três enzimas hidrolíticas, dentre elas a a-galactosidase. Além da reserva de carbono, há uma grande quantidade de corpos protéicos, no citoplasma das células endospérmicas, que constituem a principal reserva de nitrogênio nestas sementes. Para que ocorra a correta distribuição dos produtos de degradação das reservas deve haver sincronização entre os processos de degradação das reservas de carbono e nitrogênio, porém para compreender tais mecanismos, é necessário estudar aspectos do controle da produção e ação das enzimas responsáveis pela hidrólise das reservas. Buscando determinar em que ponto do metabolismo a semente de S. virgata se encontra em relação à produção destas enzimas hidrolíticas, durante e após a germinação, e supostamente os tecidos envolvidos nesta produção, sementes desta espécie foram embebidas em actinomicina-D (inibidor de transcrição) e cicloheximida (inibidor de tradução) e os efeitos destes inibidores verificados através da atividade e detecção da a-galactosidase no tegumento e endosperma destas sementes. Além disso, buscando verificar e relacionar o efeito do ácido abscísico, do etileno e dos açúcares na degradação das reservas após a germinação de S. virgata, sementes desta espécie foram embebidas em ABA, etileno, glucose e sacarose, e os efeitos destes foram verificados através dos teores protéicos, da atividade da a-galactosidase e da produção endógena de ABA e etileno nestas sementes. Como a presença de actinomicina-D e cicloheximida não inibiram a produção e a atividade da a-galactosidase no endosperma durante e após a germinação, sugere-se que a produção desta enzima ocorra principalmente durante a maturação da semente. Aparentemente, a partir do período pós-germinativo, a enzima pré-formada seria processada e ativada, para conseqüente degradação dos galactomananos, através de um processo proteolítico. Em contrapartida, como a presença deactinomicina-D e cicloheximida inibiram a degradação das proteínas de reserva no tegumento e endosperma e, inclusive, induziram a atividade da a-galactosidase nestes tecidos no final do processo de degradação dos galactomananos, sugerese a síntese de novo de proteases durante e após a germinação e a relação íntima destas enzimas com a degradação das proteínas de reserva e da a-galactosidase no final do processo de degradação dos galactomananos. Quanto aos hormônios, a presença de ABA exógeno retardou o início da degradação das proteínas de reserva no endosperma, assim como diminuiu a atividade da a-galactosidase no mesmo tecido no final do processo de degradação do galactomanano, sugerindo um efeito modulador deste hormônio durante a degradação das reservas, reprimindo a ação das enzimas hidrolíticas. A presença de etileno exógeno, entretanto, aumentou a atividade da a-galactosidase no endosperma e, inclusive, no tegumento no final do processo de degradação do galactomanano, sugerindo um efeito indutor deste hormônio na ativação e ação das enzimas hidrolíticas. De fato, analisando a produção endógena de ABA e etileno, observou-se um aumento brusco dos hormônios no período de mobilização das reservas, que pode estar relacionado à influência destes hormônios na atividade das enzimas hidrolíticas em sementes de S. virgata. Ainda, como ocorreram mudanças na produção de ABA e etileno endógeno na presença de glucose e sacarose, sugere-se uma relação íntima entre a via de sinalização destes hormônios e a dos açúcares, a fim de controlar o processo de degradação das reservas. Desta forma, estas evidências sugerem que o ABA e o etileno controlariam antagonicamente a mobilização de reservas em sementes de S. virgata, juntamente com os açúcares, através da ativação e ação das enzimas hidrolíticas, a fim de controlar o processo de degradação das proteínas e dos galactomananos, evitando a produção dos açúcares redutores e de sacarose em excesso durante o período pós-germinativo e garantindo o afluxo eficiente de carbono e nitrogênio para o desenvolvimento da plântula.
Abstract: Seeds of Sesbania virgata (Cav.) Pers. have an endosperm which accumulates galactomannan as a storage polysaccharide in the cell walls that is hydrolysed after germination by three enzymes (a-galactosidase, endo-ß-mannanase and exo-ß-mannosidase). Besides the storage of carbon, there is a great amount of protein bodies in the cytoplasm of endospermic cells, which play the major role as a nitrogen reserve in this seed. It is likely that a synchronization between the process of galactomannan and protein degradation occurs for a more efficient distribution and use of the storage degradation products. However, to understand these mechanisms, it is necessary to study aspects of the production and action control of the hydrolytic enzymes responsible for the mobilisation of these reserves. Aiming to determine in which point of the metabolism the seed of S. virgata is in relation to the production of the hydrolytic enzymes, during and after germination, and the supposed tissues involved in this production, seeds of this specie were imbibed in actinomycin-D (transcription inhibitor) and cycloheximide (translation inhibitor), and the effects of these inhibitors verified thought the a-galactosidase activity and detection in the testa and endosperm of these seeds. Besides of this, for to verify and relate the effects of abscisic acid, ethylene and sugars on the reserves degradation after germination of the S. virgata, seeds of this specie were imbibed in ABA, ethylene, glucose and sucrose, and the effects of these were verified through the protein content, a-galactosidase activity and endogenous production of ABA and ethylene in this seeds. In the presence of actinomycin-D and cycloheximide, the production and activity of the a- galactosidase were not inhibited during and after germination, suggesting that the production of this enzyme occurs principally during the maturation of the seed. Apparently, after the germination, this enzyme is processed by proteolysis, so that it becomes activated to perform galactomannan degradation. On the other hand, the presence of actinomycin-D and cycloheximide inhibited the protein degradation in the testa and endosperm and at the same time induced the a-galactosidaseactivity in the same tissues at the final steps of the galactomannan degradation process. This suggests the existence of synthesis de novo of the proteases during and after germination and a possible relationship between the storage protein and galactomannan mobilisation. Regarding the hormones, the presence of exogenous ABA retarded the beginning of the storage protein degradation in the endosperm and also decreased a-galactosidase activity in the same tissue at the end of galactomannan degradation. This suggests that there is a regulator effect of this hormone during the storage degradation, repressing the enzymes action. In the presence of exogenous ethylene an increase in the a-galactosidase activity in the endosperm and in the testa were observed at the end of galactomannan degradation, suggesting an inducing effect of this hormone on the hydrolytic enzymes. The finding of endogenous ABA and ethylene production, observed during the period of the storage mobilisation, give support to the above raised hypothesis that the two hormones participate in the control of the hydrolytic enzyme activities in seeds of S. virgata. Furthermore, the changes observed in the endogenous ABA and ethylene production in the presence of glucose and sucrose, suggested a relation between the signaling pathway of these hormones and the sugars signaling, controlling the process of storage degradation. Thus, our results add evidence to suggest that ABA and ethylene antagonistically control the storage mobilisation in seeds of S. virgata, together with the sugars, through the hydrolytic enzymes activation, controlling the process of storage protein and galactomannan degradation, in other to avoiding the excess production of reductor sugars and sucrose during the post-germinative period and assuring the efficient afflux of the carbon and nitrogen to the seedling development.
Doutorado
Biologia Celular
Doutor em Biologia Celular e Estrutural
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