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Journal articles on the topic 'A novel peptide biosensor platform'

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Author: Grafiati

Published: 10 December 2022

Last updated: 29 January 2023

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1

Gu, Yi, Qian Wen, Yongqing Kuang, Lijuan Tang, and Jianhui Jiang. "Peptide-templated gold nanoclusters as a novel label-free biosensor for the detection of protease activity." RSC Adv. 4, no. 27 (2014): 13753–56. http://dx.doi.org/10.1039/c4ra00096j.

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2

Khozeymeh, Foroogh, Federico Melli, Sabrina Capodaglio, Roberto Corradini, Fetah Benabid, Luca Vincetti, and Annamaria Cucinotta. "Hollow-Core Fiber-Based Biosensor: A Platform for Lab-in-Fiber Optical Biosensors for DNA Detection." Sensors 22, no. 14 (July 8, 2022): 5144. http://dx.doi.org/10.3390/s22145144.

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In this paper, a novel platform for lab-in-fiber-based biosensors is studied. Hollow-core tube lattice fibers (HC-TLFs) are proposed as a label-free biosensor for the detection of DNA molecules. The particular light-guiding mechanism makes them a highly sensitive tool. Their transmission spectrum is featured by alternations of high and low transmittance at wavelength regions whose values depend on the thickness of the microstructured web composing the cladding around the hollow core. In order to achieve DNA detection by using these fibers, an internal chemical functionalization process of the fiber has been performed in five steps in order to link specific peptide nucleic acid (PNA) probes, then the functionalized fiber was used for a three-step assay. When a solution containing a particular DNA sequence is made to flow through the HC of the TLF in an ‘optofluidic’ format, a bio-layer is formed on the cladding surfaces causing a red-shift of the fiber transmission spectrum. By comparing the fiber transmission spectra before and after the flowing it is possible to identify the eventual formation of the layer and, therefore, the presence or not of a particular DNA sequence in the solution.

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3

Er, Simge, Ushna Laraib, Rabia Arshad, Saman Sargazi, Abbas Rahdar, Sadanand Pandey, Vijay Kumar Thakur, and Ana M. Díez-Pascual. "Amino Acids, Peptides, and Proteins: Implications for Nanotechnological Applications in Biosensing and Drug/Gene Delivery." Nanomaterials 11, no. 11 (November 8, 2021): 3002. http://dx.doi.org/10.3390/nano11113002.

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Over various scientific fields in biochemistry, amino acids have been highlighted in research works. Protein, peptide- and amino acid-based drug delivery systems have proficiently transformed nanotechnology via immense flexibility in their features for attaching various drug molecules and biodegradable polymers. In this regard, novel nanostructures including carbon nanotubes, electrospun carbon nanofibers, gold nanoislands, and metal-based nanoparticles have been introduced as nanosensors for accurate detection of these organic compounds. These nanostructures can bind the biological receptor to the sensor surface and increase the surface area of the working electrode, significantly enhancing the biosensor performance. Interestingly, protein-based nanocarriers have also emerged as useful drug and gene delivery platforms. This is important since, despite recent advancements, there are still biological barriers and other obstacles limiting gene and drug delivery efficacy. Currently available strategies for gene therapy are not cost-effective, and they do not deliver the genetic cargo effectively to target sites. With rapid advancements in nanotechnology, novel gene delivery systems are introduced as nonviral vectors such as protein, peptide, and amino acid-based nanostructures. These nano-based delivery platforms can be tailored into functional transformation using proteins and peptides ligands based nanocarriers, usually overexpressed in the specified diseases. The purpose of this review is to shed light on traditional and nanotechnology-based methods to detect amino acids, peptides, and proteins. Furthermore, new insights into the potential of amino protein-based nanoassemblies for targeted drug delivery or gene transfer are presented.

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4

Antonescu, Oana N., Andreas Rasmussen, Nicole A. M. Damm, Ditte F. Heidemann, Roman Popov, Alexander Nesterov-Mueller, Kristoffer E. Johansson, and Jakob R. Winther. "Substitutional landscape of a split fluorescent protein fragment using high-density peptide microarrays." PLOS ONE 16, no. 2 (February 3, 2021): e0241461. http://dx.doi.org/10.1371/journal.pone.0241461.

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Split fluorescent proteins have wide applicability as biosensors for protein-protein interactions, genetically encoded tags for protein detection and localization, as well as fusion partners in super-resolution microscopy. We have here established and validated a novel platform for functional analysis of leave-one-out split fluorescent proteins (LOO-FPs) in high throughput and with rapid turnover. We have screened more than 12,000 variants of the beta-strand split fragment using high-density peptide microarrays for binding and functional complementation in Green Fluorescent Protein. We studied the effect of peptide length and the effect of different linkers to the solid support. We further mapped the effect of all possible amino acid substitutions on each position as well as in the context of some single and double amino acid substitutions. As all peptides were tested in 12 duplicates, the analysis rests on a firm statistical basis allowing for confirmation of the robustness and precision of the method. Based on experiments in solution, we conclude that under the given conditions, the signal intensity on the peptide microarray faithfully reflects the binding affinity between the split fragments. With this, we are able to identify a peptide with 9-fold higher affinity than the starting peptide.

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5

Ronda, Luca, Alessandro Tonelli, Elisa Sogne, Ida Autiero, Francesca Spyrakis, Sara Pellegrino, Giorgio Abbiati, et al. "Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection of Staphylococcus aureus." Sensors 20, no. 17 (September 2, 2020): 4977. http://dx.doi.org/10.3390/s20174977.

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The urgent need to develop a detection system for Staphylococcus aureus, one of the most common causes of infection, is prompting research towards novel approaches and devices, with a particular focus on point-of-care analysis. Biosensors are promising systems to achieve this aim. We coupled the selectivity and affinity of aptamers, short nucleic acids sequences able to recognize specific epitopes on bacterial surface, immobilized at high density on a nanostructured zirconium dioxide surface, with the rational design of specifically interacting fluorescent peptides to assemble an easy-to-use detection device. We show that the displacement of fluorescent peptides upon the competitive binding of S. aureus to immobilized aptamers can be detected and quantified through fluorescence loss. This approach could be also applied to the detection of other bacterial species once aptamers interacting with specific antigens will be identified, allowing the development of a platform for easy detection of a pathogen without requiring access to a healthcare environment.

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6

Sfragano, Patrick Severin, Giulia Moro, Federico Polo, and Ilaria Palchetti. "The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics." Biosensors 11, no. 8 (July 23, 2021): 246. http://dx.doi.org/10.3390/bios11080246.

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Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.

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7

Kirgoz, Ülkü A, Suna Timur, Dilek Odaci, Briza Pérez, Salvador Alegret, and Arben Merkoçi. "Carbon Nanotube Composite as Novel Platform for Microbial Biosensor." Electroanalysis 19, no. 7-8 (April 2007): 893–98. http://dx.doi.org/10.1002/elan.200603786.

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8

Wodnicka, Magdalena, Richard D. Guarino, John J. Hemperly, Mark R. Timmins, David Stitt, and J. Bruce Pitner. "Novel Fluorescent Technology Platform for High Throughput Cytotoxicity and Proliferation Assays." Journal of Biomolecular Screening 5, no. 3 (June 2000): 141–52. http://dx.doi.org/10.1177/108705710000500306.

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We have developed a novel fluorescent Oxygen BioSensor technology platform adaptable to many applications in the area of drug discovery and development, particularly cell-based assays. This biosensor technology requires no additional reagents or incubations, and affords continuous real-time readout of dissolved oxygen concentrations. Since the level of oxygen dissolved in an assay's medium correlates to the number and viability of the cells in the medium, this technology is ideally suited for monitoring cell viability, proliferation, or death. The technology is particularly well suited to investigating cells' kinetic responses to proliferative or toxic stimuli, such as drugs. When incorporated into a 96- or 384-well microplate format, it is compatible with standard laboratory automation systems. Here we present data illustrating the application of the Oxygen BioSensor technology for rapid, homogeneous detection and evaluation of metabolic activity of a variety of eukaryotic and prokaryotic cells, including mammalian cells, insect cells, yeast, and bacteria. In the absence of toxic substances, we find a good correlation between cell number and signal over a wide range of cell concentrations and growth times. To evaluate the usefulness of the Oxygen BioSensor for cytotoxicity assays, we have performed a series of experiments using a range of toxic agents and cell types, including both bacteria and mammalian cell lines. In a side-by-side comparison to standard MTT assays using HL60 cells, comparable IC50 values were found with the Oxygen BioSensor for five different toxins or drugs. This assay method does not have the need for additional reagents, handling steps, or incubation periods required by the MTT assays.

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9

Soylemez, Saniye, Tuğçe Yılmaz, Ece Buber, Yasemin A. Udum, Salih Özçubukçu, and Levent Toppare. "Polymerization and biosensor application of water soluble peptide-SNS type monomer conjugates." Journal of Materials Chemistry B 5, no. 35 (2017): 7384–92. http://dx.doi.org/10.1039/c7tb01674c.

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10

Wang, Jianxiu, Ding Li, Minghui Yang, and Yi Zhang. "A novel ferrocene-tagged peptide nanowire for enhanced electrochemical glucose biosensing." Anal. Methods 6, no. 18 (2014): 7161–65. http://dx.doi.org/10.1039/c4ay01604a.

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11

Eissa, Shimaa, and Mohammed Zourob. "A dual electrochemical/colorimetric magnetic nanoparticle/peptide-based platform for the detection of Staphylococcus aureus." Analyst 145, no. 13 (2020): 4606–14. http://dx.doi.org/10.1039/d0an00673d.

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12

Park, Byung-Wook, Rui Zheng, Kyoung-A. Ko, Brent D. Cameron, Do-Young Yoon, and Dong-Shik Kim. "A novel glucose biosensor using bi-enzyme incorporated with peptide nanotubes." Biosensors and Bioelectronics 38, no. 1 (October 2012): 295–301. http://dx.doi.org/10.1016/j.bios.2012.06.005.

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13

Liu, Xiaochen, Lihao Wang, Junyuan Zhao, Yinfang Zhu, Jinling Yang, and Fuhua Yang. "Enhanced Binding Efficiency of Microcantilever Biosensor for the Detection of Yersinia." Sensors 19, no. 15 (July 29, 2019): 3326. http://dx.doi.org/10.3390/s19153326.

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A novel microcantilever sensor was batch fabricated for Yersinia detection. The microcantilever surface modification method was optimized by introducing a secondary antibody to increase the number of binding sites. A novel microfluidic platform was designed and fabricated successfully. A 30 μL solution could fully react with the microcantilever surface. Those routines enhanced the binding efficiency between the target and receptor on the microcantilever. With this novel designed microfluidic platform, the specific adsorption of 107 Yersinia on the beam surface with modified F1 antibody was significantly enhanced.

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14

Peng, Yu-Chi, Chia-Hsuan Cheng, Hiromi Yatsuda, Szu-Heng Liu, Shih-Jen Liu, Takashi Kogai, Chen-Yen Kuo, and Robert Y. L. Wang. "A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)." Diagnostics 11, no. 10 (October 5, 2021): 1838. http://dx.doi.org/10.3390/diagnostics11101838.

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Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375–1100 ng/mL) of the “spike-in” anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 (p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing.

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15

Hua, Zulin, Qin Qin, Xue Bai, Xin Huang, and Qi Zhang. "An electrochemical biosensing platform based on 1-formylpyrene functionalized reduced graphene oxide for sensitive determination of phenol." RSC Advances 6, no. 30 (2016): 25427–34. http://dx.doi.org/10.1039/c5ra27563f.

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16

Glatz, Richard V., Wayne R. Leifert, Tamara H. Cooper, Kelly Bailey, Chris S. Barton, A. Scott Martin, Amanda L. Aloia, et al. "Molecular Engineering of G Protein-Coupled Receptors and G Proteins for Cell-Free Biosensing." Australian Journal of Chemistry 60, no. 5 (2007): 309. http://dx.doi.org/10.1071/ch06435.

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The ability to express and purify modified recombinant proteins, so they retain their biological function in a cell-free format, has provided a basis for development of molecular biosensors. Here we utilize recombinant G Protein-coupled receptors (GPCRs) and their G proteins for cell-free detection of various binding partners. Fusion peptides were used to improve surface-attachment and fluorescent-labelling capabilities. A novel homogeneous fluorescence resonance energy transfer (FRET)-based assay was developed to detect rearrangements in the G protein heterotrimer. By using this heterotrimeric ‘molecular switch’, we are developing a generic technology such that multiple GPCRs could be assayed for ligand-mediated activation while tethered to surfaces or in solution, with increased throughput compared to current assay platforms.

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17

Yemini, Miri, Meital Reches, Judith Rishpon, and Ehud Gazit. "Novel Electrochemical Biosensing Platform Using Self-Assembled Peptide Nanotubes." Nano Letters 5, no. 1 (January 2005): 183–86. http://dx.doi.org/10.1021/nl0484189.

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18

Lantigua, Darlin, Jamie Trimper, Baris Unal, and Gulden Camci-Unal. "A new paper-based biosensor for therapeutic drug monitoring." Lab on a Chip 21, no. 17 (2021): 3289–97. http://dx.doi.org/10.1039/d1lc00473e.

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19

Kribich, K. R., R. Copperwhite, H. Barry, B. Kolodziejczyk, J. M. Sabattié, K. O’Dwyer, and B. D. MacCraith. "Novel chemical sensor/biosensor platform based on optical multimode interference (MMI) couplers." Sensors and Actuators B: Chemical 107, no. 1 (May 2005): 188–92. http://dx.doi.org/10.1016/j.snb.2004.11.098.

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20

Yu, Yanyan, Peng Wang, Xiaodan Zhu, Qiwen Peng, Yi Zhou, Tianxiao Yin, Yixin Liang, and Xiaoxing Yin. "Combined determination of copper ions and β-amyloid peptide by a single ratiometric electrochemical biosensor." Analyst 143, no. 1 (2018): 323–31. http://dx.doi.org/10.1039/c7an01683b.

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21

Kim, Chuntae, Hansong Lee, Vasanthan Devaraj, Won-Geun Kim, Yujin Lee, Yeji Kim, Na-Na Jeong, et al. "Hierarchical Cluster Analysis of Medical Chemicals Detected by a Bacteriophage-Based Colorimetric Sensor Array." Nanomaterials 10, no. 1 (January 9, 2020): 121. http://dx.doi.org/10.3390/nano10010121.

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M13 bacteriophage-based colorimetric sensors, especially multi-array sensors, have been successfully demonstrated to be a powerful platform for detecting extremely small amounts of target molecules. Colorimetric sensors can be fabricated easily using self-assembly of genetically engineered M13 bacteriophage which incorporates peptide libraries on its surface. However, the ability to discriminate many types of target molecules is still required. In this work, we introduce a statistical method to efficiently analyze a huge amount of numerical results in order to classify various types of target molecules. To enhance the selectivity of M13 bacteriophage-based colorimetric sensors, a multi-array sensor system can be an appropriate platform. On this basis, a pattern-recognizing multi-array biosensor platform was fabricated by integrating three types of sensors in which genetically engineered M13 bacteriophages (wild-, RGD-, and EEEE-type) were utilized as a primary building block. This sensor system was used to analyze a pattern of color change caused by a reaction between the sensor array and external substances, followed by separating the specific target substances by means of hierarchical cluster analysis. The biosensor platform could detect drug contaminants such as hormone drugs (estrogen) and antibiotics. We expect that the proposed biosensor system could be used for the development of a first-analysis kit, which would be inexpensive and easy to supply and could be applied in monitoring the environment and health care.

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22

Korkmaz, Nuriye, Changhyun Hwang, Kim Kristin Kessler, Yuliya E. Silina, Lisann Müller, and Jayoung Park. "A novel copper (II) binding peptide for a colorimetric biosensor system design." Talanta 232 (September 2021): 122439. http://dx.doi.org/10.1016/j.talanta.2021.122439.

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23

Xue, Tianyu, Sudhakara Reddy Bongu, Hao Huang, Weiyuan Liang, Yingwei Wang, Feng Zhang, Zhongyuan Liu, Yupeng Zhang, Han Zhang, and Xiaoqiang Cui. "Ultrasensitive detection of microRNA using a bismuthene-enabled fluorescence quenching biosensor." Chemical Communications 56, no. 51 (2020): 7041–44. http://dx.doi.org/10.1039/d0cc01004a.

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24

Zimina, Tatiana, Vladimir Karasev, Viktor Luchinin, Alexander Kolobov, Ivan Mandrik, Nikita Sitkov, Liudmila Kraeva, Natalia Shevchenko, and Yuri Orekhov. "Peptide-Based Biosensor for Express Diagnostics of Coronavirus Respiratory Infections." Proceedings 60, no. 1 (November 2, 2020): 52. http://dx.doi.org/10.3390/iecb2020-07059.

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At the end of year 2019 the first reports appeared of a new coronavirus and on 31st December 2019 WHO declared a public health emergency of international concern. To date (as of 6:08 pm CET, 24th November 2020) according to WHO the new coronavirus, now called severe acute respiratory syndrome (SARS)-CoV-2, has infected 58,900,547 people and killed 1,393,305 people worldwide. It is extremely important to develop means for express diagnostics to ensure prompt action to limit the spread of infection. One of the diagnostic approaches, is the detection of viral particles in swabs. This approach can be realized using a biosensor with specific ligands, based on peptide molecules complementary to surface viral proteins. The concept of the so-called Systems of Conjugated Ionic-Hydrogen Bonds (abbreviated—SSIVS, CIHBS) implemented in the Protein-3D computer program, was applied to analyze the spatial structures of the bonds between the SARS-CoV-2 spike protein and the ACE-2 (Angiotensin converting enzyme 2) receptor, in order to reveal the perspective peptide sequences. There are two clearly marked areas of contact of the spike with the cell receptor—upper and lower, which are visualized in the SSIVS form, and the complex formed at this site is strong enough to ensure its attachment to the coronavirus spike and can compete for binding with the ACE-2 receptor. Two peptides were developed that form a spatial structure complementary to the coronavirus spike: of eight (No. one) and of 15 (No. two) amino acid residues. The peptides were covalently bound to biochip platforms via neutral linkers to form sites with peptides No. one and No. two. The third site has a neutral hydrophilic surface to serve as a reference. The platform was integrated with a microfluidic channel and was used as a flow through device. The detection of bound viral particles was carried out using UV excitation and direct registration of viral proteins fluorescence. The preliminary laboratory tests demonstrated the efficiency of the biosensor.

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25

Patra, Snehangshu, Tania Hidalgo Crespo, Anastasia Permyakova, Clémence Sicard, Christian Serre, Annie Chaussé, Nathalie Steunou, and Ludovic Legrand. "Design of metal organic framework–enzyme based bioelectrodes as a novel and highly sensitive biosensing platform." Journal of Materials Chemistry B 3, no. 46 (2015): 8983–92. http://dx.doi.org/10.1039/c5tb01412c.

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26

Rad-Malekshahi, Mazda, Marieke F. Fransen, Małgorzata Krawczyk, Mercedeh Mansourian, Meriem Bourajjaj, Jian Chen, Ferry Ossendorp, Wim E. Hennink, Enrico Mastrobattista, and Maryam Amidi. "Self-Assembling Peptide Epitopes as Novel Platform for Anticancer Vaccination." Molecular Pharmaceutics 14, no. 5 (January 27, 2017): 1482–93. http://dx.doi.org/10.1021/acs.molpharmaceut.6b01003.

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27

Bertrand, Yanick, Jean-Christophe Currie, Michel Demeule, Anthony Régina, Christian Ché, Abedelnasser Abulrob, Dorothy Fatehi, et al. "Transport characteristics of a novel peptide platform for CNS therapeutics." Journal of Cellular and Molecular Medicine 14, no. 12 (December 2010): 2827–39. http://dx.doi.org/10.1111/j.1582-4934.2009.00930.x.

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28

Arotiba, Omotayo A., Anna Ignaszak, Rehana Malgas, Amir Al-Ahmed, Priscilla G. L. Baker, Selwyn F. Mapolie, and Emmanuel I. Iwuoha. "An electrochemical DNA biosensor developed on novel multinuclear nickel(II) salicylaldimine metallodendrimer platform." Electrochimica Acta 53, no. 4 (December 2007): 1689–96. http://dx.doi.org/10.1016/j.electacta.2007.08.016.

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29

Bhardwaj, Rahul, Ngashangva Lightson, Yoshiaki Ukita, and Yuzuru Takamura. "Development of oligopeptide-based novel biosensor by solid-phase peptide synthesis on microchip." Sensors and Actuators B: Chemical 192 (March 2014): 818–25. http://dx.doi.org/10.1016/j.snb.2013.10.086.

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30

Mahmood, Hafiz Zeeshan, Asim Jilani, Sajid Farooq, Yasir Javed, Yasir Jamil, Javed Iqbal, Sami Ullah, and Swelm Wageh. "Plasmon-Based Label-Free Biosensor Using Gold Nanosphere for Dengue Detection." Crystals 11, no. 11 (November 2, 2021): 1340. http://dx.doi.org/10.3390/cryst11111340.

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In this study, a novel label-free immunosensor platform is developed to exploit the localized surface plasmon resonance (LSPR) phenomenon. The LSPR solution-based platform is designed by a gold nanospheres probe, functionalized with monoclonal anti-dengue antibody (IgG). Numerical calculations are performed to assess the LSPR extinction spectrum and spatial near electric field distribution around the nanoparticle surface. Important parameters that govern sensor performance, molecular and refractive index sensitivity are evaluated. On the evaluation of the platform as a molecular sensor, the detection of dengue NS1 antigens is presented. The results are consistent with the numerical simulations, which depicts the system’s ability to identify dengue NS1 antigen concentrations as low as 0.07 ± 0.01 µg/mL, along with fosters its potential application in plasmonic sensing.

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Solra, Manju, Rajni Bala, Nishima Wangoo, Gurpreet K. Soni, Munish Kumar, and Rohit K. Sharma. "Optical pico-biosensing of lead using plasmonic gold nanoparticles and a cationic peptide-based aptasensor." Chemical Communications 56, no. 2 (2020): 289–92. http://dx.doi.org/10.1039/c9cc07407d.

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A novel biosensor for the rapid detection of lead ions employing the optical properties of AuNPs, a lead-specific aptamer and a cationic peptide has been demonstrated with ultra-sensitive detection limit.

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Cui, Wei, and Laurie L. Parker. "A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity." Chemical Communications 51, no. 2 (2015): 362–65. http://dx.doi.org/10.1039/c4cc07453j.

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33

Soylemez, Saniye, Seza Goker, and Levent Toppare. "A newly designed anthracene and isoindigo based polymer: synthesis, electrochemical characterization and biosensor applications." New Journal of Chemistry 43, no. 35 (2019): 13979–84. http://dx.doi.org/10.1039/c9nj02546d.

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A novel sensing platform based on a newly designed and synthesized polymer, poly[(E)-6-methyl-6′-(10-methylanthracen-9-yl)-1,1′-diundecyl-[3,3′-biindolinylidene]-2,2′-dione] (namely PIIDAnth), was fabricated and explored as a glucose amperometric biosensor.

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34

Mas-Moruno, Carlos, Roberta Fraioli, Fernando Albericio, José María Manero, and F. Javier Gil. "Novel Peptide-Based Platform for the Dual Presentation of Biologically Active Peptide Motifs on Biomaterials." ACS Applied Materials & Interfaces 6, no. 9 (April 15, 2014): 6525–36. http://dx.doi.org/10.1021/am5001213.

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35

Kim, Soo Min, Taek Lee, Yeong-Gyu Gil, Ga Hyeon Kim, Chulhwan Park, Hongje Jang, and Junhong Min. "Fabrication of Bioprobe Self-Assembled on Au–Te Nanoworm Structure for SERS Biosensor." Materials 13, no. 14 (July 21, 2020): 3234. http://dx.doi.org/10.3390/ma13143234.

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In the present study, we propose a novel biosensor platform using a gold-tellurium (Au–Te) nanoworm structure through surface-enhanced Raman spectroscopy (SERS). Au–Tenanoworm was synthesized by spontaneous galvanic replacement of sacrificial Te nanorods templated with Au (III) cations under ambient conditions. The fabricated Au–Te nanoworm exhibited an interconnected structure of small spherical nanoparticles and was found to be effective at enhancing Raman scattering. The Au–Te nanoworm-immobilized substrate exhibited the ability to detect thyroxine using an aptamer-tagged DNA three-way junction (3WJ) and glycoprotein 120 (GP120) human immunodeficiency virus (HIV) using an antibody. The modified substrates were investigated by scanning electron microscopy and atomic force microscopy (AFM). The optimal Au–Te nanoworm concentration and immobilization time for the thyroxine biosensor platform were further determined by SERS experimentation. Thus, the present study showed that the Au–Te nanoworm structure could be applied to various biosensor platforms.

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36

Wang, Xin, Mengjie Gu, Tan Boon Toh, Nurrul Lissa Binti Abdullah, and Edward Kai-Hua Chow. "Stimuli-Responsive Nanodiamond-Based Biosensor for Enhanced Metastatic Tumor Site Detection." SLAS TECHNOLOGY: Translating Life Sciences Innovation 23, no. 1 (October 11, 2017): 44–56. http://dx.doi.org/10.1177/2472630317735497.

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Metastasis is often critical to cancer progression and linked to poor survival and drug resistance. Early detection of metastasis, as well as identification of metastatic tumor sites, can improve cancer patient survival. Thus, developing technology to improve the detection of cancer metastasis biomarkers can improve both diagnosis and treatment. In this study, we investigated the use of nanodiamonds to develop a stimuli-responsive metastasis detection complex that utilizes matrix metalloproteinase 9 (MMP9) as a metastasis biomarker, as MMP9 increased expression has been shown to be indicative of metastasis. The nanodiamond–MMP9 biosensor complex consists of nanodiamonds functionalized with MMP9-specific fluorescent-labeled substrate peptides. Using this design, protease activity of MMP9 can be accurately measured and correlated to MMP9 expression. The nanodiamond–MMP9 biosensor also demonstrated an enhanced ability to protect the base sensor peptide from nonspecific serum protease cleavage. This enhanced peptide stability, combined with a quantitative stimuli-responsive output function, provides strong evidence for the further development of a nanodiamond–MMP9 biosensor for metastasis site detection. More importantly, this work provides the foundation for use of nanodiamonds as a platform for stimuli-responsive biosensors and theranostic complexes that can be implemented across a wide range of biomedical applications.

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Xu, Huiying, Chong Liao, Shifu Liang, and Bang-Ce Ye. "A Novel Peptide-Equipped Exosomes Platform for Delivery of Antisense Oligonucleotides." ACS Applied Materials & Interfaces 13, no. 9 (February 23, 2021): 10760–67. http://dx.doi.org/10.1021/acsami.1c00016.

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Dervisevic, Muamer, Esma Dervisevic, Emre Çevik, and Mehmet Şenel. "Novel electrochemical xanthine biosensor based on chitosan–polypyrrole–gold nanoparticles hybrid bio-nanocomposite platform." Journal of Food and Drug Analysis 25, no. 3 (July 2017): 510–19. http://dx.doi.org/10.1016/j.jfda.2016.12.005.

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Li, Da-Wei, Lei Luo, Peng-Fei Lv, Qing-Qing Wang, Ke-Yu Lu, An-Fang Wei, and Qu-Fu Wei. "Synthesis of Polydopamine Functionalized Reduced Graphene Oxide-Palladium Nanocomposite for Laccase Based Biosensor." Bioinorganic Chemistry and Applications 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/5360361.

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Graphene based 2D nanomaterials have attracted increasing attention in biosensing application due to the outstanding physicochemical properties of graphene. In this work, palladium nanoparticles (Pd) loaded reduced graphene oxide (rGO) hybrid (rGO-Pd) was synthesized through a facile method. Laccase (Lac) was immobilized on rGO-Pd by utilizing the self-polymerization of dopamine, which generated polydopamine (PDA). The PDA-Lac-rGO-Pd nanocomposites were further modified on electrode surface to construct novel biosensing platform. The obtained electrochemical biosensor was applied in the detection of catechol, achieving excellent analytic results. Under the optimum condition, this biosensor possessed a linear range from 0.1 µM to 263 µM for catechol detection, the sensitivity reached 18.4 µA mM−1, and the detection limit was as low as 0.03 µM. In addition, the biosensor also showed good repeatability, reproducibility, anti-interference, and stability. Moreover, the novel Lac based biosensor was successfully used in the trace detection of catechol existing in real water environment.

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Smith, Dustin D., Joshua P. King, D. Wade Abbott, and Hans-Joachim Wieden. "Development of a Real-Time Pectic Oligosaccharide-Detecting Biosensor Using the Rapid and Flexible Computational Identification of Non-Disruptive Conjugation Sites (CINC) Biosensor Design Platform." Sensors 22, no. 3 (January 26, 2022): 948. http://dx.doi.org/10.3390/s22030948.

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Fluorescently labeled, solute-binding proteins that change their fluorescent output in response to ligand binding are frequently used as biosensors for a wide range of applications. We have previously developed a “Computational Identification of Non-disruptive Conjugation sites” (CINC) approach, an in silico pipeline utilizing molecular dynamics simulations for the rapid design and construction of novel protein–fluorophore conjugate-type biosensors. Here, we report an improved in silico scoring algorithm for use in CINC and its use in the construction of an oligogalacturonide-detecting biosensor set. Using both 4,5-unsaturated and saturated oligogalacturonides, we demonstrate that signal transmission from the ligand-binding pocket of the starting protein scaffold to the CINC-selected reporter positions is effective for multiple different ligands. The utility of an oligogalacturonide-detecting biosensor is shown in Carbohydrate Active Enzyme (CAZyme) activity assays, where the biosensor is used to follow product release upon polygalacturonic acid (PGA) depolymerization in real time. The oligogalacturonide-detecting biosensor set represents a novel enabling tool integral to our rapidly expanding platform for biosensor-based carbohydrate detection, and moving forward, the CINC pipeline will continue to enable the rational design of biomolecular tools to detect additional chemically distinct oligosaccharides and other solutes.

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Xiang, Qian, Ying Gao, Jing Qiu Liu, Kun Qi Wang, Juan Tang, Ming Yang, Shu Ping Wang, and Wei Ling Wang. "Development of Nanomaterials Electrochemical Biosensor and its Applications." Advanced Materials Research 418-420 (December 2011): 2082–85. http://dx.doi.org/10.4028/www.scientific.net/amr.418-420.2082.

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Study of the electrochmeical biosensor has become a new interdisciplinary frontier between biological detection and material science due to their excellent prospects for interfacing biological recognition events with electronic signal transduction. Nanomaterials provided a significant platform for designing a new generation of bioelectronic devices exhibiting novel functions due to their high surface-to-volume ratio, good stability, small dimension effect, good compatibility and strong adsorption ability. In this paper, we review the development of electrochemical biosensors fabricated with various nanoscale materials, also highlight the analytical applications in terms of biochemistry.

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Yang, Xiaoli, Wei Wei, Jianhui Jiang, Guoli Shen, and Ruqin Yu. "Conformational switching of G-quadruplexes as a label-free platform for the fluorescence detection of Ag+ and biothiols." Analytical Methods 8, no. 2 (2016): 311–15. http://dx.doi.org/10.1039/c5ay02632f.

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Kim, Hyun, Ju Hye Jang, In Young Jung, and Ju Hyun Cho. "A Novel Peptide as a Specific and Selective Probe for Klebsiella pneumoniae Detection." Biosensors 12, no. 3 (March 1, 2022): 153. http://dx.doi.org/10.3390/bios12030153.

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Klebsiella pneumoniae is infamous for generating hospital-acquired infections, many of which are difficult to treat due to the bacterium’s multidrug resistance. A sensitive and robust detection method of K. pneumoniae can help prevent a disease outbreak. Herein, we used K. pneumoniae cells as bait to screen a commercially available phage-displayed random peptide library for peptides that could be used to detect K. pneumoniae. The biopanning-derived peptide TSATKFMMNLSP, named KP peptide, displayed a high selectivity for the K. pneumoniae with low cross-reactivity to related Gram-negative bacteria. The specific interaction between KP peptide and K. pneumoniae lipopolysaccharide resulted in the peptide’s selectivity against K. pneumoniae. Quantitative analysis of this interaction by enzyme-linked immunosorbent assay revealed that the KP peptide possessed higher specificity and sensitivity toward K. pneumoniae than commercially available anti-Klebsiella spp. antibodies and could detect K. pneumoniae at a detection limit of 104 CFU/mL. These results suggest that KP peptide can be a promising alternative to antibodies in developing a biosensor system for K. pneumoniae detection.

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Pei, Qianqian, Yu Wang, Su Liu, Yifei Qin, Xueqi Leng, Xuejun Cui, and Jiadong Huang. "Exonuclease III-aided autonomous cascade signal amplification: a facile and universal DNA biosensing platform for ultrasensitive electrochemical detection of S. typhimurium." New Journal of Chemistry 41, no. 15 (2017): 7613–20. http://dx.doi.org/10.1039/c7nj01626c.

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Liu, Qingtao, Yunxin Xiao, Adrian Hawley, and Ben J. Boyd. "Lipid-based lyotropic liquid crystalline phase transitions as a novel assay platform using birefringence as the visual signal output." Journal of Materials Chemistry B 8, no. 29 (2020): 6277–85. http://dx.doi.org/10.1039/d0tb00355g.

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Paiva Maia, Yara Cristina, Thaise Gonçalves Araújo, Diego Leoni Franco, Fausto Emilio Capparelli, Vanesssa Silva Ribeiro, Patrícia Tieme Fujimura, Luanda Calábria, et al. "A novel breast cancer screening platform: An epitope-based biomarker coupled to electrochemical sensor." Journal of Clinical Oncology 33, no. 28_suppl (October 1, 2015): 9. http://dx.doi.org/10.1200/jco.2015.33.28_suppl.9.

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9 Background: The subtractive proteomic selection technology called Phage Display (PD) has been extensively used by our group in the discovery of high affinity ligands to target tissues and molecules. We have selected specific ligands against IgG purified from BC tissues, which was successfully coupled to an electrochemical sensor to detect the tumor-specific immune response. Methods: After PD selections, all immunoreactive peptide ligands were further characterized by DNA sequencing, in vitro translated and submitted to bioinformatic analyses. Further validations were performed by ELISA. We then used one synthetic peptide (SF4) for the construction of an immunosensor, which was applied to patients and control samples for final validation. Electrochemical impedance spectroscopy (EIS) was performed. Results: We have selected the F4 peptide for final validation and sensor construction due to its capability of detecting IgG in the peripheral blood and the excellent ELISA ratio BC:BBD, discriminating more than 70% of BC patients. The synthetic peptide reached a good precision in BC diagnosis (68%), but surprisingly, the selected F4 clone presented the highest sensitivity and specificity, (77.8% and 85.7%, respectively), suggesting that it can be used as a diagnostic reagent for early BC screening prior to imaging and pathological analyses. The electrochemical sensor that was built with the epitope-based peptide discriminated all IgG from BC and healthy individuals. Results with the EIS sensor demonstrated that the presence of (SF4) peptide IgG generated higher resistivity (-Z ') compared to the system containing only the peptide or the control sera. This is justified by the fact that with the immobilized biological peptide layer was correctly conjugated with the IgG forming an antigen: antibody complex that led to an increased resistance to the charge transfer system, producing a decrease in electron transfer between the iron-coupled / ferricyanide redox and the electrode surface. Conclusions: An electrochemical sensor using an epitope-based biomarker was developed, which allows for the first time BC screening using a very simple platform that could be an important auxiliary tool to mammography imaging.

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Ben Halima, Hamdi, Nadia Zine, Joan Bausells, Nicole Jaffrezic-Renault, and Abdelhamid Errachid. "A Novel Cortisol Immunosensor Based on a Hafnium Oxide/Silicon Structure for Heart Failure Diagnosis." Micromachines 13, no. 12 (December 16, 2022): 2235. http://dx.doi.org/10.3390/mi13122235.

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Assessing cortisol levels in human bodies has become essential to diagnose heart failure (HF). In this work, we propose a salivary cortisol detection strategy as part of an easily integrable lab-on-a-chip for detection of HF biomarkers. Our developed capacitive immunosensor based on hafnium oxide (HfO2)/silicon structure showed good linearity between increasing cortisol concentration and the charge-transfer resistance/capacitance. Moreover, the developed biosensor was demonstrated to be highly selective toward cortisol compared to other HF biomarkers such as tumor necrosis factor (TNF-α) and N-terminal pro-brain natriuretic peptide (NT-proBNP). The precision of our developed biosensor was evaluated, and the difference between the determined cortisol concentration in saliva and its expected one is <18%.

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Sitkov, N. O., T. M. Zimina, V. V. Luchinin, A. A. Kolobov, A. A. Romanov, Yu D. Orekhov, A. V. Korlyakov, A. A. Ryabko, O. A. Naretskaya, and M. I. Kiseleva. "Hybrid-Integrated Biosensor for Express Determination of Protein Markers of Diseases based on Molecular Recognition and Direct Fluorimetric Detection." Nano- i Mikrosistemnaya Tehnika 23, no. 6 (December 23, 2021): 326–32. http://dx.doi.org/10.17587/nmst.23.326-332.

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Ways of creating new generation biosensors for multiparametric express diagnostics based on molecular recognition and direct fluorimetric registration of a peptide aptamer — protein marker complex were considered. The biosensor platform comprises a microfluidic channel for delivery sample solutions, coupled with flow-through zones containing covalently attached arrays of peptide probes — aptamers. An outer glass window of the biochip assembly contains a layer of luminophore ZnS:Cu, bound on it via an acrylic lacquer and intended for the re-emitting native fluorescence of bound proteins into the longer wavelength range, more efficient in registering signals with CMOS sensors. The aptamers were designed using "Protein 3D" program for analysis of spatial complementarity of protein structures. The peptide, complementary to Troponin T, was modified by replacement of aromatic amino acid residue while maintaining the spatial configuration. The complementarity of peptide and Troponin T was confirmed using a capillary electrophoresis-on-a-chip. Biosensors are manufactured using thick-film technology and photolithography. The fluorescence of marker proteins was excited using UV-LED with a radiation wavelength of 275 nm. The limit of detection achieved for Troponin T was 6 ng/ml.

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Boobphahom, Siraprapa, Pranee Rattanawaleedirojn, Yuttanant Boonyongmaneerat, Sirirat Rengpipat, Orawon Chailapakul, and Nadnudda Rodthongkum. "TiO2 sol/graphene modified 3D porous Ni foam: A novel platform for enzymatic electrochemical biosensor." Journal of Electroanalytical Chemistry 833 (January 2019): 133–42. http://dx.doi.org/10.1016/j.jelechem.2018.11.031.

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Case, Allison, Angela Desmond, Kate Jiyeun Yi, Pavitra Chakravarty, Joan Smallshaw, Angela Collins, Kelly Dye, et al. "A novel platform to generate peptoid-based mimetic vaccines (P4504)." Journal of Immunology 190, no. 1_Supplement (May 1, 2013): 178.6. http://dx.doi.org/10.4049/jimmunol.190.supp.178.6.

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Abstract The aim of this research is to generate vaccine candidates for any virus for which a neutralizing monoclonal antibody exists without prior knowledge of the protective epitope. We have developed a platform to generate such vaccine candidates and are working on a device to automate their identification. The platform consists of libraries of B cell epitopes prepared by displaying peptoid sequences on beads, screening with neutralizing monoclonal antibodies to select peptoids bound by the antigen binding site of the monoclonal antibody, and retaining the antibody-bound peptoids with protein G dynabeads and a magnet. Peptoids are similar to peptides but with the R group attached to the nitrogen instead of the carbon. They are haptens that can be attached to protein carriers to elicit anti-peptoid antibody responses. We have conducted platform optimization and proof-of-principle testing using FLAG peptide and anti-FLAG monoclonal antibody. We then applied the optimized platform to screen peptoid libraries with monoclonal anti-FLAG and identified two potential mimetics. Mice immunized with the peptoids have made FLAG peptide-reactive antibodies, thereby demonstrating proof of concept. Implementation of this platform with neutralizing monoclonal antibodies we have in hand against HIV, West Nile virus and hepatitis C virus can identify potential mimetic vaccine candidates without prior knowledge of important epitopes, and could represent an entirely new way to generate safe vaccines.

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