Academic literature on the topic 'A novel peptide biosensor platform'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'A novel peptide biosensor platform.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "A novel peptide biosensor platform"
1
Gu, Yi, Qian Wen, Yongqing Kuang, Lijuan Tang, and Jianhui Jiang. "Peptide-templated gold nanoclusters as a novel label-free biosensor for the detection of protease activity." RSC Adv. 4, no. 27 (2014): 13753–56. http://dx.doi.org/10.1039/c4ra00096j.
Full text
2
Khozeymeh, Foroogh, Federico Melli, Sabrina Capodaglio, Roberto Corradini, Fetah Benabid, Luca Vincetti, and Annamaria Cucinotta. "Hollow-Core Fiber-Based Biosensor: A Platform for Lab-in-Fiber Optical Biosensors for DNA Detection." Sensors 22, no. 14 (July 8, 2022): 5144. http://dx.doi.org/10.3390/s22145144.
Full textAbstract:
In this paper, a novel platform for lab-in-fiber-based biosensors is studied. Hollow-core tube lattice fibers (HC-TLFs) are proposed as a label-free biosensor for the detection of DNA molecules. The particular light-guiding mechanism makes them a highly sensitive tool. Their transmission spectrum is featured by alternations of high and low transmittance at wavelength regions whose values depend on the thickness of the microstructured web composing the cladding around the hollow core. In order to achieve DNA detection by using these fibers, an internal chemical functionalization process of the fiber has been performed in five steps in order to link specific peptide nucleic acid (PNA) probes, then the functionalized fiber was used for a three-step assay. When a solution containing a particular DNA sequence is made to flow through the HC of the TLF in an ‘optofluidic’ format, a bio-layer is formed on the cladding surfaces causing a red-shift of the fiber transmission spectrum. By comparing the fiber transmission spectra before and after the flowing it is possible to identify the eventual formation of the layer and, therefore, the presence or not of a particular DNA sequence in the solution.
3
Er, Simge, Ushna Laraib, Rabia Arshad, Saman Sargazi, Abbas Rahdar, Sadanand Pandey, Vijay Kumar Thakur, and Ana M. Díez-Pascual. "Amino Acids, Peptides, and Proteins: Implications for Nanotechnological Applications in Biosensing and Drug/Gene Delivery." Nanomaterials 11, no. 11 (November 8, 2021): 3002. http://dx.doi.org/10.3390/nano11113002.
Full textAbstract:
Over various scientific fields in biochemistry, amino acids have been highlighted in research works. Protein, peptide- and amino acid-based drug delivery systems have proficiently transformed nanotechnology via immense flexibility in their features for attaching various drug molecules and biodegradable polymers. In this regard, novel nanostructures including carbon nanotubes, electrospun carbon nanofibers, gold nanoislands, and metal-based nanoparticles have been introduced as nanosensors for accurate detection of these organic compounds. These nanostructures can bind the biological receptor to the sensor surface and increase the surface area of the working electrode, significantly enhancing the biosensor performance. Interestingly, protein-based nanocarriers have also emerged as useful drug and gene delivery platforms. This is important since, despite recent advancements, there are still biological barriers and other obstacles limiting gene and drug delivery efficacy. Currently available strategies for gene therapy are not cost-effective, and they do not deliver the genetic cargo effectively to target sites. With rapid advancements in nanotechnology, novel gene delivery systems are introduced as nonviral vectors such as protein, peptide, and amino acid-based nanostructures. These nano-based delivery platforms can be tailored into functional transformation using proteins and peptides ligands based nanocarriers, usually overexpressed in the specified diseases. The purpose of this review is to shed light on traditional and nanotechnology-based methods to detect amino acids, peptides, and proteins. Furthermore, new insights into the potential of amino protein-based nanoassemblies for targeted drug delivery or gene transfer are presented.
4
Antonescu, Oana N., Andreas Rasmussen, Nicole A. M. Damm, Ditte F. Heidemann, Roman Popov, Alexander Nesterov-Mueller, Kristoffer E. Johansson, and Jakob R. Winther. "Substitutional landscape of a split fluorescent protein fragment using high-density peptide microarrays." PLOS ONE 16, no. 2 (February 3, 2021): e0241461. http://dx.doi.org/10.1371/journal.pone.0241461.
Full textAbstract:
Split fluorescent proteins have wide applicability as biosensors for protein-protein interactions, genetically encoded tags for protein detection and localization, as well as fusion partners in super-resolution microscopy. We have here established and validated a novel platform for functional analysis of leave-one-out split fluorescent proteins (LOO-FPs) in high throughput and with rapid turnover. We have screened more than 12,000 variants of the beta-strand split fragment using high-density peptide microarrays for binding and functional complementation in Green Fluorescent Protein. We studied the effect of peptide length and the effect of different linkers to the solid support. We further mapped the effect of all possible amino acid substitutions on each position as well as in the context of some single and double amino acid substitutions. As all peptides were tested in 12 duplicates, the analysis rests on a firm statistical basis allowing for confirmation of the robustness and precision of the method. Based on experiments in solution, we conclude that under the given conditions, the signal intensity on the peptide microarray faithfully reflects the binding affinity between the split fragments. With this, we are able to identify a peptide with 9-fold higher affinity than the starting peptide.
5
Ronda, Luca, Alessandro Tonelli, Elisa Sogne, Ida Autiero, Francesca Spyrakis, Sara Pellegrino, Giorgio Abbiati, et al. "Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection of Staphylococcus aureus." Sensors 20, no. 17 (September 2, 2020): 4977. http://dx.doi.org/10.3390/s20174977.
Full textAbstract:
The urgent need to develop a detection system for Staphylococcus aureus, one of the most common causes of infection, is prompting research towards novel approaches and devices, with a particular focus on point-of-care analysis. Biosensors are promising systems to achieve this aim. We coupled the selectivity and affinity of aptamers, short nucleic acids sequences able to recognize specific epitopes on bacterial surface, immobilized at high density on a nanostructured zirconium dioxide surface, with the rational design of specifically interacting fluorescent peptides to assemble an easy-to-use detection device. We show that the displacement of fluorescent peptides upon the competitive binding of S. aureus to immobilized aptamers can be detected and quantified through fluorescence loss. This approach could be also applied to the detection of other bacterial species once aptamers interacting with specific antigens will be identified, allowing the development of a platform for easy detection of a pathogen without requiring access to a healthcare environment.
6
Sfragano, Patrick Severin, Giulia Moro, Federico Polo, and Ilaria Palchetti. "The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics." Biosensors 11, no. 8 (July 23, 2021): 246. http://dx.doi.org/10.3390/bios11080246.
Full textAbstract:
Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.
7
Kirgoz, Ülkü A, Suna Timur, Dilek Odaci, Briza Pérez, Salvador Alegret, and Arben Merkoçi. "Carbon Nanotube Composite as Novel Platform for Microbial Biosensor." Electroanalysis 19, no. 7-8 (April 2007): 893–98. http://dx.doi.org/10.1002/elan.200603786.
Full text
8
Wodnicka, Magdalena, Richard D. Guarino, John J. Hemperly, Mark R. Timmins, David Stitt, and J. Bruce Pitner. "Novel Fluorescent Technology Platform for High Throughput Cytotoxicity and Proliferation Assays." Journal of Biomolecular Screening 5, no. 3 (June 2000): 141–52. http://dx.doi.org/10.1177/108705710000500306.
Full textAbstract:
We have developed a novel fluorescent Oxygen BioSensor technology platform adaptable to many applications in the area of drug discovery and development, particularly cell-based assays. This biosensor technology requires no additional reagents or incubations, and affords continuous real-time readout of dissolved oxygen concentrations. Since the level of oxygen dissolved in an assay's medium correlates to the number and viability of the cells in the medium, this technology is ideally suited for monitoring cell viability, proliferation, or death. The technology is particularly well suited to investigating cells' kinetic responses to proliferative or toxic stimuli, such as drugs. When incorporated into a 96- or 384-well microplate format, it is compatible with standard laboratory automation systems. Here we present data illustrating the application of the Oxygen BioSensor technology for rapid, homogeneous detection and evaluation of metabolic activity of a variety of eukaryotic and prokaryotic cells, including mammalian cells, insect cells, yeast, and bacteria. In the absence of toxic substances, we find a good correlation between cell number and signal over a wide range of cell concentrations and growth times. To evaluate the usefulness of the Oxygen BioSensor for cytotoxicity assays, we have performed a series of experiments using a range of toxic agents and cell types, including both bacteria and mammalian cell lines. In a side-by-side comparison to standard MTT assays using HL60 cells, comparable IC50 values were found with the Oxygen BioSensor for five different toxins or drugs. This assay method does not have the need for additional reagents, handling steps, or incubation periods required by the MTT assays.
9
Soylemez, Saniye, Tuğçe Yılmaz, Ece Buber, Yasemin A. Udum, Salih Özçubukçu, and Levent Toppare. "Polymerization and biosensor application of water soluble peptide-SNS type monomer conjugates." Journal of Materials Chemistry B 5, no. 35 (2017): 7384–92. http://dx.doi.org/10.1039/c7tb01674c.
Full text
10
Wang, Jianxiu, Ding Li, Minghui Yang, and Yi Zhang. "A novel ferrocene-tagged peptide nanowire for enhanced electrochemical glucose biosensing." Anal. Methods 6, no. 18 (2014): 7161–65. http://dx.doi.org/10.1039/c4ay01604a.
Full textDissertations / Theses on the topic "A novel peptide biosensor platform"
1
Goujon, Jennyfer. "Towards the development of a novel colourimetric nucleic acid biosensor based on peptide nucleic acid-functionalised polydiacetylene liposomes." Thesis, Heriot-Watt University, 2009. http://hdl.handle.net/10399/2306.
Full textAbstract:
The aim of the research project described here was to develop a novel colourimetric nucleic acids biosensor based on polydiacetylene liposomes containing lipophilic peptide nucleic acids. Preliminary investigations in this area have shown that PNA-containing PDA liposomes can be constructed and that they are blue in colour as expected. However, their poor water solubility and the resulting precipitation made it necessary to synthesise and evaluate a second generation. In these, the PNA head-group is separated from the lipid tail by an amino diethylene glycol-type spacer molecule. The first hydrophilic spacer synthesised was 8-(tert-butoxycarbonyl)amino-3,6- dioxaoctanoic acid. Three methods based on the O-alkylation of mono-Boc, di-Boc and dibenzyl 5-amino-3-oxapentanol were devised. The Boc strategy afforded the corresponding ether in low yield of 20% while in the case of the dibenzyl approach a suitable methodology to effectively cleave the protecting groups from the amino function could not be found. Moreover, the model reaction between dibenzyl 8-amino-3,6- dioxaoctanoic acid and thyminyl PNA monomer afforded the corresponding conjugates in only 6%. An alternative type of connection to link the spacer to the PNA headgroup was thus sought. A 1,4-disubstituted [1,2,3]-triazole moiety was obtained in 60% yield by reacting spacer, 8-(tert-butoxycarbonyl)amino-3,6-dioxaoctan-1-azide and N-alkynyl PNA monomers bearing thymine, Cbz protected adenine and Cbz protected cytosine according to the conditions of the ‘Click’ reaction. These ‘spacer-PNA monomer’ intermediates were then functionalised at their N-terminus using 10,12-pentacosadiynoyl fluoride, to afford ‘lipid-spacer-PNA monomer’ models. The saturated conjugates were obtained by first preparing the ‘lipid-spacer’ intermediate, N-(8-azido-3,6- dioxaoctanyl)stearamide which was then connected to the N-alkynyl PNA monomers using the ‘Click’ reaction. Following this last strategy, a homothymine PNA dimer, prepared in solution phase, was also functionalised with the saturated lipid chain. This second generation of lipid functionalised-PNA monomer models were incorporated into PDA-liposomes. Upon photo-polymerisation, the liposome solutions became blue. They were found to be stable in solution, at 4 oC over long period of time. Their colour changed to red upon environmental changes (i.e. pH and temperature).
2
Nausch, Lydia. "Novel Insights into PKG Activation and cGMP Signaling in Response to Nitric Oxide and Atrial Natriuretic Peptide in Vascular Smooth Muscle Cells." ScholarWorks @ UVM, 2008. http://scholarworks.uvm.edu/graddis/158.
Full textAbstract:
Cyclic 3',5'-guanosine monophosphate (cGMP) is a key signaling molecule involved in a myriad of physiological processes, including vascular smooth muscle (VSM) tone, water- and electrolyte homeostasis, platelet aggregation, airway smooth muscle tone, smooth muscle proliferation and bone formation. Increased occurrence of vascular disorders including erectile dysfunction, hypertension, stroke and coronary artery disease, have made it increasingly important to study the dynamic interplay between cGMP synthesis and hydrolysis in VSM cells. This dissertation examines the spatial distribution of intracellular cGMP, [cGMP]i, in response to NO and atrial natriuretic peptide (ANP) in VSM cells. To investigate the spatial patterning of [cGMP]i, we have developed a new generation of non-FRET (fluorescence resonance energy transfer) cGMP biosensors that are suitable to monitor [cGMP]i in response to physiological (low-nanomolar) NO and ANP concentrations and that qualify for real-time, confocal imaging techniques. We have termed these indicators FlincGs, for green fluorescent indicators of cGMP. For the development of FlincGs, we made use of the specific cGMP binding characteristics of PKG. We utilized site-specific mutagenesis, kinetic cGMP binding, dissociation and kinase assays, as well as crystallography, in order to investigate PKG activation and cGMP binding dynamics in greater detail. Based on these studies, our novel, non-FRET cGMP biosensors were designed by attaching cGMP binding fragments of PKG to the N-terminus of circular permutated green fluorescent protein. We applied FlincGs in cultured VSM cells as well as in intact tissue to determine whether two spatially distinct populations of guanlylyl cyclase (cytosolic versus membrane bound) underlie the generation of spatiotemporally-specific patterns of [cGMP]i formation.
3
Carpenter, Sarah Elizabeth. "Enzyme linked spectroscopic assays for Glyoxylate the use of Peptidylglycine alpha-Amidating Monoxygenase for the discovery of Novel alpha-Amidated hormones /." [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001415.
Full text
4
Abdul, Rahman Mohd Syaifudin Bin. "Novel sensor design for detection of dangerous contaminated marine biotoxins : a thesis submitted in fulfilment of the requirements for the degree of Master of Engineering in Information and Telecommunication Engineering, School of Engineering and Advanced Technology, Massey University, Palmerston North, New Zealand." Massey University, 2009. http://hdl.handle.net/10179/1007.
Full textAbstract:
Planar electromagnetic sensing system has been used as one of the NDT methods to evaluate the material properties i.e., to evaluate near-surface properties such as conductivity, permeability and dielectric properties. The applications of planar electromagnetic sensors will depend on both the characteristic of the sensor type chosen and also the characteristic of material under test. Conventional planar interdigital sensors and novel planar interdigital sensors have been designed, fabricated and tested for detection of dangerous marine biotoxins in seafood. Our main objective is to sense the presence of dangerous contaminated acid in mussels and other seafoods. Initial studies were conducted with three peptide derivatives namely Sarcosine, Proline and Hydroxylproline. These three chemicals are structurally closely related to our target molecule (domoic acid). The initial results have shown that all sensors respond very well to the chemicals and it is possible to discriminate the different chemicals from the output of the sensor. Novel interdigital sensors have shown better sensitivity measurement compared to conventional interdigital sensors. The novel interdigital sensors were then being tested with three seafood products. Results from the analysis have shown that novel interdigital sensor with configuration #1 (Sensor_1) has better sensitivity compared to other sensors. Sensor_1 has been chosen for experiment using proline and mussels. The changes in sensor sensitivity were analysed with mussels before and after adding the proline. The presence of proline on the mussel surface and also injected proline to the mussel samples were clearly detected by Sensor_1. Further experiment was conducted with small amount of domoic acid (0.5 µg to 5.0 µg) injected to a mussel and it was found that Sensor_1 was able to detect small amount of domoic acid (1.0 µg) injected into the mussel sample. Sensor_1 was able to detect approximately 12.6 µg/g of domoic acid in mussel meat. Three threshold levels of particular sample thickness have been established for detection of domoic acid. The first prototype of a low cost sensing system known as SIT (Seafood Inspection Tool) has been developed. The outcomes from the experiments provide chances of opportunity for further research in developing a low cost miniature type of sensors for reliable sensing system for commercial use.
5
"Characterization and Analysis of a Novel Platform for Profiling the Antibody Response." Doctoral diss., 2011. http://hdl.handle.net/2286/R.I.14273.
Full textAbstract:
abstract: Immunosignaturing is a new immunodiagnostic technology that uses random-sequence peptide microarrays to profile the humoral immune response. Though the peptides have little sequence homology to any known protein, binding of serum antibodies may be detected, and the pattern correlated to disease states. The aim of my dissertation is to analyze the factors affecting the binding patterns using monoclonal antibodies and determine how much information may be extracted from the sequences. Specifically, I examined the effects of antibody concentration, competition, peptide density, and antibody valence. Peptide binding could be detected at the low concentrations relevant to immunosignaturing, and a monoclonal's signature could even be detected in the presences of 100 fold excess naive IgG. I also found that peptide density was important, but this effect was not due to bivalent binding. Next, I examined in more detail how a polyreactive antibody binds to the random sequence peptides compared to protein sequence derived peptides, and found that it bound to many peptides from both sets, but with low apparent affinity. An in depth look at how the peptide physicochemical properties and sequence complexity revealed that there were some correlations with properties, but they were generally small and varied greatly between antibodies. However, on a limited diversity but larger peptide library, I found that sequence complexity was important for antibody binding. The redundancy on that library did enable the identification of specific sub-sequences recognized by an antibody. The current immunosignaturing platform has little repetition of sub-sequences, so I evaluated several methods to infer antibody epitopes. I found two methods that had modest prediction accuracy, and I developed a software application called GuiTope to facilitate the epitope prediction analysis. None of the methods had sufficient accuracy to identify an unknown antigen from a database. In conclusion, the characteristics of the immunosignaturing platform observed through monoclonal antibody experiments demonstrate its promise as a new diagnostic technology. However, a major limitation is the difficulty in connecting the signature back to the original antigen, though larger peptide libraries could facilitate these predictions.Dissertation/Thesis
Ph.D. Molecular and Cellular Biology 2011
Book chapters on the topic "A novel peptide biosensor platform"
1
Mathivanan, Sivaji. "Perspectives of Nano-Materials and Nanobiosensors in Food Safety and Agriculture." In Novel Nanomaterials. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.95345.
Full textAbstract:
Nanobiosensor is one type of biosensor made up with usage of nanomaterials i.e., nanoparticles and nanostructures. Because of the nanomaterials’ unique properties such as good conductivity, and physicochemical, electrochemical, optical, magnetic and mechanical properties, Nanobiosensors are highly reliable and more sensitive in biosensing approaches over conventional sensors which is having various limitation in detection. Quantum dots, nanotubes, nanowires, magnetic and other nanoparticles enhance sensitivity and lower limit of detection by amplifying signals and providing novel signal transduction mechanisms enable detection of a very low level of food contaminants, pesticides, foodborne pathogens, toxins and plant metabolites. Nanobiosensors are having a lot of scope in sustainable agriculture because of its detecting ability i.e., sensing changes occurred in molecular level. So it can be utilized to find out the variations or modification of plant metabolities, volatiles, gas exchange, hormonal and ion concentration etc. which are the indicators of various harsh environmental stresses (abiotic), biotic and physiological stress. Identification of the stress in the starting stage itself will help us to avoid intensive plant damage and prevent yield losses created by the stress. Nanosensors can be used in smart farming, in which all the environmental factors related to plant growth like temperature, water, pH, humidity, nutritional factor etc. are measured and precaution taken to control the factors which reduce the crop production with the help of IOT platform, thereby enhance the productivity. In this review, discussed about nanobiosensors for detection of food contaminants and various application and its potential in agriculture.
2
Anton Okhai, Timothy, Azeez O. Idris, Usisipho Feleni, and Lukas W. Snyman. "Nanomaterial-Enhanced Receptor Technology for Silicon On-Chip Biosensing Application." In Biosensor - Current and Novel Strategies for Biosensing [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.94249.
Full textAbstract:
Nanomaterials integration in biosensors designs are known to enhance sensing and signaling capabilities by exhibiting remarkably high surface area enhancement and intrinsic reactivity owing to their distinctive optical, chemical, electrical and catalytic properties. We present the synthesis and characterization of silver nanoparticles (AgNPs), and their immobilization on a silicon on-chip biosensor platform to enhance sensing capability for prostate specific antigen (PSA) - cancer biomarkers. Several techniques, including UV-Visible (UV-Vis) absorption spectrum, Fourier transforms infrared spectroscopy (FTIR), high resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) were used for characterizing the AgNPs. The biochemical sensor consists of AgNPs immobilized on the receptor layer of a silicon avalanche mode light emitting device (Si AM LED) which enables on-chip optical detection biological analytes. A bio-interaction layer etched from the chip interacts with the evanescent field of a micro dimensioned waveguide. An array of detectors below the receptor cavity selectively monitor reflected light in the UV, visible, infrared and far infrared wavelength regions. AgNPs used as an immobilization layer in the receptor layer enhances selective absorption analytes, causing a change in detection signal as a function of propagation wavelength as light is dispersed. The analytes could range from gases to cancer biomarkers like prostate specific antigen.
3
Pourasl, Ali Hosseingholi, Mohammad Taghi Ahmadi, Meisam Rahmani, Razali Ismail, and Michael Loong Pengl Tan. "Graphene and CNT Field Effect Transistors Based Biosensor Models." In Handbook of Research on Nanoelectronic Sensor Modeling and Applications, 294–333. IGI Global, 2017. http://dx.doi.org/10.4018/978-1-5225-0736-9.ch012.
Full textAbstract:
In this chapter, novel ideas of graphene and CNT based electrical biosensors are provided. A liquid gated graphene field effect transistor (LG-GFET) based biosensor model is analytically developed for electrical detection of Escherichia coli (E. coli) bacteria. E. coli absorption effects on the graphene surface in the form of conductance variation is considered. Moreover, the current-voltage characteristic in terms of conductance model is applied to evaluate the performance of the biosensor model. Furthermore, the CNT-FET platform is employed for modeling biosensor in order to detect Glucose. For diagnosing and monitoring the blood glucose level, glucose oxidase (GOx) based enzyme sensors have been immensely used. According to the proposed CNT-FET structure, charge based analytical modeling approach is used. The charge-based carrier velocity model is implemented to study electrical characteristics of CNT-FET. In the presented model, the gate voltage is considered as a function of glucose concentration. Finally, the both of presented models are compared with published experimental data.
4
Jaswal, Radhika R., Kanica Kaushal, Shubhi Joshi, Pratibha Sharma, Shweta Sharma, Simran Preet, and Avneet Saini. "Exploring the Potential of Peptides and Peptidomimetics in Biosensing." In Advances in Medical Diagnosis, Treatment, and Care, 33–65. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-0307-2.ch003.
Full textAbstract:
Biosensors are devices that capture the biological signal and convert it into a detectable electrical signal through transduction. Biological entities like DNA, RNA, and proteins/enzymes can be conjugated onto the biosensor surface to detect and observe certain biological analytes in environment, biomedical, and food industries. Peptides have been efficiently used in the fabrication of peptide-based biosensors due to their attractive properties like established synthesis protocols, diverse structures, and as highly enzyme-selective substrates. However, owing to their labile nature, peptidomimetics are the best alternatives at the bioreceptor interface due to their specificity and stability, relatively low cost and easy modifications, and capability to form supramolecular assemblies like nanosheets. Such bioconjugation strategies efficiently convert interaction information into a measurable signal, thus highlighting the importance in the fabrication of next-generation novel robust biosensors desirable for detection and dissemination of pathogens causing infections in the living and non-living worlds.
5
Singleton, Rana, Carrie Sanders, and Alain B. Waffo. "Application of Phage Biotechnology in Nanobiotechnology." In Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering, 36–48. IGI Global, 2015. http://dx.doi.org/10.4018/978-1-4666-6363-3.ch003.
Full textAbstract:
To date, the phage display system has enabled the discovery of material binding peptides. Diversity and functionality of these peptides could be improved using RNA-based display systems instead of the conventional DNA-based ones. RNA phage replication systems possess unique features that make them a versatile tool for any combinatory approach and evolutionary application. Phage display was used to monitor the chemical surface properties and to initiate nanoparticle assembly. Novel bio-panning was recently used in RNA-based display to screen new functionality without acidic elution used in other conventional DNA phage display systems. Therefore, Hybrid RNA phages would be a perfect platform for attachment and exploration of nanoparticles. In this chapter, the authors present an overview on research conducted on these cross fields and areas. They not only focus on the novel selection and amplification process but also on the importance of RNA phage and its peptide display as tools for preventing nanoparticle aggregation.
6
Singleton, Rana, Carrie Sanders, and Alain B. Waffo. "Application of Phage Biotechnology in Nanobiotechnology." In Medical Imaging, 1151–64. IGI Global, 2017. http://dx.doi.org/10.4018/978-1-5225-0571-6.ch047.
Full textAbstract:
To date, the phage display system has enabled the discovery of material binding peptides. Diversity and functionality of these peptides could be improved using RNA-based display systems instead of the conventional DNA-based ones. RNA phage replication systems possess unique features that make them a versatile tool for any combinatory approach and evolutionary application. Phage display was used to monitor the chemical surface properties and to initiate nanoparticle assembly. Novel bio-panning was recently used in RNA-based display to screen new functionality without acidic elution used in other conventional DNA phage display systems. Therefore, Hybrid RNA phages would be a perfect platform for attachment and exploration of nanoparticles. In this chapter, the authors present an overview on research conducted on these cross fields and areas. They not only focus on the novel selection and amplification process but also on the importance of RNA phage and its peptide display as tools for preventing nanoparticle aggregation.
Conference papers on the topic "A novel peptide biosensor platform"
1
Song, Taehyeon, Minwoo Nam, SeungYeon Song, Hyun C. Yoon, and Keekeun Lee. "A novel wireless Love wave biosensor platform for multifunctional detection." In SPIE MOEMS-MEMS, edited by Sonia Garcia-Blanco and Rajeshuni Ramesham. SPIE, 2011. http://dx.doi.org/10.1117/12.876098.
Full text
2
Weilbaecher, Craig R., Maruf Hossain, Shubhra Gangopadhyay, and Sheila A. Grant. "Development of a novel nanomaterial-based optical platform for a protease biosensor." In Optics East 2007, edited by Brian M. Cullum and D. Marshall Porterfield. SPIE, 2007. http://dx.doi.org/10.1117/12.734679.
Full text
3
Veisi, Zeinab, Muhammet Ceylan, Anil Mahapatro, and Ramazan Asmatulu. "An Electrospun Polyaniline Nanofiber as a Novel Platform for Real-Time COX-2 Biomarker Detection." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-65269.
Full textAbstract:
The presence of Cyclooxygenase-2 (COX-2) biomarker has been associated with the development of certain types of cancer such as breast cancer. Moreover, reliable quantification of COX-2 as an enzyme responsible for pain and inflammation is vital. Here we demonstrate the feasibility of sensitive COX-2 detection via integration of nanoporous polyaniline fibers on the microfabricated platform to develop a label-free biosensor. Highly porous polyaniline nanofibers were fabricated in different diameters and integrated on the interdigitated microelectrodes to develop electrochemical platforms. Characterization results revealed that the smaller diameter improved the sensitivity of the biosensor due to enhancement in the specific surface area. The developed biosensor was able to detect analyte as low as 0.1pg/mL with a large dynamic linear range of 10fg/mL to 1μg/mL. The fabricated sensor showed remarkable sensitivity towards COX-2 antigen suggesting the significant contribution of this nanofiber based platform to the enhanced sensitivity in COX-2 analyte detection.
4
Silva, L. B., P. Baptista, L. Raniero, G. Doria, R. Franco, R. Martins, and E. Fortunato. "Novel Optoelectronic Platform using an Amorphous/Nanocrystalline Silicon Biosensor for the Specific Identification of Unamplified Nucleic Acid Sequences Based on Gold Nanoparticle Probes." In TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference. IEEE, 2007. http://dx.doi.org/10.1109/sensor.2007.4300285.
Full text
5
Ahmad, Asad, Nathan Gallant, Rasim Guldiken, and Onursal Onen. "Surface Functionalization of an Ovarian Cancer Diagnostic Biosensor." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-64311.
Full textAbstract:
Ovarian cancer is the fifth leading cause of death among women in United States and the disease has 1.4% (1 in 71) lifetime risk. Patients with ovarian cancer have a short median survival time after diagnosis with their 5-year survival rate being less than 40%. Early stage ovarian cancer represents an important target for screening since it is lethal in most late stage cases (1). Currently the primary screening procedure for ovarian cancer are blood levels of cancer antigen (CA) 125, however CA 125 levels can also be elevated due to other disorders and do not provide conclusive results (2). Utilizing the research done at the Cell and Molecular Biology department at the University of South Florida which conclusively revealed that urinary levels of bcl-2 are elevated in ovarian cancer patients (3), this research it the first of its kind looking to assess the capture of an analyte protein on a series of potential bioconjugated surfaces for use in a novel acoustic biosensor. Therefore, this research addresses the need for a reliable and economic testing platform to detect ovarian cancer.
6
Liu, Fei, Anis N. Nordin, Fang Li, and Ioana Voiculescu. "A Sensitive Multiparametric Biosensor With Capabilities of Rapid Toxicity Detection of Drinking Water." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-62281.
Full textAbstract:
Recently, there has been interest to develop biosensors based on live mammalian cells to monitor the toxicity of water. The cell viability after exposure to toxic water can be monitored by electric cell-substrate impedance sensing (ECIS) of the cell membrane. However, these impedance based toxicity sensors can only provide one single sensing endpoint (impedance measurement), and many toxicants cannot be detected at the concentration between Military Exposure Guideline levels and estimated Human Lethal Concentrations. The goal of this paper is to provide a rapid and sensitive sensing platform for long-term water toxicity detection. In this paper a novel multiparametric biosensor with integrated microfluidic channels for water toxicity detection is presented. Toxicity tests to study bovine aortic endothelial cells (BAECs) responsiveness to health-threatening concentrations of ammonia in de-ionized (DI) water will be presented. We demonstrated the BAECs can rapidly respond to ammonia concentrations between the military exposure guideline of 2mM and human lethal concentration of 55mM. The successful testing of water toxicity by simultaneous gravimetric and impedimetric measurements indicates that the multiparametric biosensor platform is able to perform rapid and sensitive detection of water toxicity and minimize the false-positive rate.
7
Ku, Yu-Fu, Yi-Kuang Yen, Ping-Yen Lin, and Long-Sun Huang. "Self Thermal Elimination of Nanomechanics-Based Biosensor Using a Single Free-Standing Piezoresistive Microcantilever." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13142.
Full textAbstract:
A compact piezoresistive microcantilever platform integrated by MEMS technique provides a convenient and reliable approach for biochemical detection. In contrast to the conventional double free-standing piezoresistive cantilever beams of heterogeneous sensing surfaces, a single free-standing piezoresistive microcantilever sensor was first proposed with the elimination of interference of chemical effect. The single beam piezoresistive sensor which is temperature-sensitive was well controlled within ±0.2°C by introducing the novel method of self elimination of thermal effect. This new approach maintains device compactness with no additional use of bulky temperature-controlled apparatus, and significantly reduces noises of temperature coefficient of resistance (TCR) and bimorph effect. The C-reactive protein (CRP) detection of the microcantilever biosensor with self elimination of thermal effect was verified with the concentration of 100 μg/mL in a room-temperature environment.
8
Radha Shanmugam, Nandhinee, Sriram Muthukumar, and Shalini Prasad. "Zinc Oxide Nanostructures as Electrochemical Biosensors on Flexible Substrates." In ASME 2015 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/smasis2015-9085.
Full textAbstract:
A novel flexible electrochemical biosensor for protein biomarker detection was successfully designed and fabricated on a nanoporous polyimide membrane using zinc oxide (ZnO). Nanostructures of ZnO were grown on microelectrode platform using aqueous solution bath. Electrochemical measurements were performed using gold, ZnO seed and nanostructured electrodes to study the influence of electrode surface area on biosensing performance. Feasibility analysis of sensor platforms was evaluated using high concentrations (in ng/mL) of troponin-T. The results showed that improved performance can be obtained on nanostructured platform by careful optimization of growth conditions. This study demonstrates the development of nanostructured ZnO flexible biosensors towards ultra-sensitive protein biosensing.
9
Onen, Onursal, Patricia Kruk, and Rasim Guldiken. "Design of Urinary Biomarker Sensor for Early Ovarian Cancer Detection." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-62818.
Full textAbstract:
In this paper, our efforts on the design, surface functionalization and characterization of ultrasonic MEMS sensor for early ovarian cancer is presented. The sensor detects urinary anti-apoptotic protein Bcl-2 level that has been presented as being elevated for different stages of ovarian cancer. Our novel biosensor approach employs a pair of MEMS ultrasound transducers for generating and sensing surface acoustic waves and a delay path in-between with oriented Bcl-2 antibodies (C8C) attached. Piezoelectric surface acoustic wave devices are employed for sensor for their high coupling efficiency and ease of fabrication. The sensor quantifies the cancer progression by detecting mass loading change generated by adhesion of Bcl-2 molecules to antibodies on the sensor surface. The device is fabricated using common MEMS fabrication techniques and a multi-step surface functionalization is utilized for effective protein adhesion. As a result, our biosensor platform has various unique advantages such as: ultra-sensitive (sub pg/ml), low cost, and simple operation (reminiscent of a pregnancy test) not necessitating trained personnel.
10
Dutta, S., and D. Banerjee. "Characterization of Micro-Valves for Lab-on-Chip Device." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-42587.
Full textAbstract:
The objective of this study is to develop a portable hand held diagnostics platform for monitoring pollutants and water quality testing. We are developing a lab-on-chip (LOC) device for in-situ synthesis of gold nano-particles and for using a colorimetric peptide assay for water quality monitoring. The gold nano-particles are synthesized in-situ in our experiments. The gold nano-particles exhibit various optical properties due to their Surface Plasmon Resonance (SPR). These stabilized mono-disperse gold nano-particles are coated with bio-molecular recognition motifs on their surfaces. The stabilization and functionalization with bio-molecular recognition motif provides flexibility for various applications. For example, the gold nano-particles synthesized by this process are tested for their ability to be recognized by a surface coated with anti-Flg antibodies. The LOC consists of micro-wells housing different reagents and samples that feed to a common reaction chamber. The reaction products are delivered to several waste chambers in a pre-defined sequence to enable subsequent reagents/ samples to flow into the reaction chamber. Passive flow actuation is obtained by capillary driven flow (wicking). Dissolvable micro-structures are used as passive micro-valves that actuate at predefined intervals and do not require any external power source for actuation. The microfluidic chip (LOC) and the dissolvable microstructures are fabricated using soft lithography techniques. The passive valves are incorporated into the microfluidics platform by novel micro-fabrication and bonding techniques.
Reports on the topic "A novel peptide biosensor platform"
1
Gurevitz, Michael, William A. Catterall, and Dalia Gordon. face of interaction of anti-insect selective toxins with receptor site-3 on voltage-gated sodium channels as a platform for design of novel selective insecticides. United States Department of Agriculture, December 2013. http://dx.doi.org/10.32747/2013.7699857.bard.
Full textAbstract:
Voltage-gated sodium channels (Navs) play a pivotal role in excitability and are a prime target of insecticides like pyrethroids. Yet, these insecticides are non-specific due to conservation of Navs in animals, raising risks to the environment and humans. Moreover, insecticide overuse leads to resistance buildup among insect pests, which increases misuse and risks. This sad reality demands novel, more selective, insect killers whose alternative use would avoid or reduce this pressure. As highly selective insect toxins exist in venomous animals, why not exploit this gift of nature and harness them in insect pest control? Many of these peptide toxins target Navs, and since their direct use via transformed crop plants or mediator microorganisms is problematic in public opinion, we focus on the elucidation of their receptor binding sites with the incentive of raising knowledge for design of toxin peptide mimetics. This approach is preferred nowadays by agro-industries in terms of future production expenses and public concern. However, characterization of a non-continuous epitope, that is the channel receptor binding site for such toxins, requires a suitable experimental system. We have established such a system within more than a decade and reached the stage where we employ a number of different insect-selective toxins for the identification of their receptor sites on Navs. Among these toxins we wish to focus on those that bind at receptor site-3 and inhibit Nav inactivation because: (1) We established efficient experimental systems for production and manipulation of site-3 toxins from scorpions and sea anemones. These peptides vary in size and structure but compete for site-3 on insect Navs. Moreover, these toxins exhibit synergism with pyrethroids and with other channel ligands; (2) We determined their bioactive surfaces towards insect and mammalian receptors (see list of publications); (3) We found that despite the similar mode of action on channel inactivation, the preference of the toxins for insect and mammalian channel subtypes varies greatly, which can direct us to structural features in the basis of selectivity; (4) We have identified by channel loop swapping and point mutagenesis extracellular segments of the Navinvolved with receptor site-3. On this basis and using channel scanning mutagenesis, neurotoxin binding, electrophysiological analyses, and structural data we offer: (i) To identify the residues that form receptor site-3 at insect and mammalian Navs; (ii) To identify by comparative analysis differences at site-3 that dictate selectivity toward various Navs; (iii) To exploit the known toxin structures and bioactive surfaces for modeling their docking at the insect and mammalian channel receptors. The results of this study will enable rational design of novel anti-insect peptide mimetics with minimized risks to human health and to the environment. We anticipate that the release of receptor site-3 molecular details would initiate a worldwide effort to design peptide mimetics for that site. This will establish new strategies in insect pest control using alternative insecticides and the combined use of compounds that interact allosterically leading to increased efficiency and reduced risks to humans or resistance buildup among insect pests.
2
Tucker, Mark L., Shimon Meir, Amnon Lers, Sonia Philosoph-Hadas, and Cai-Zhong Jiang. Elucidation of signaling pathways that regulate ethylene-induced leaf and flower abscission of agriculturally important plants. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597929.bard.
Full textAbstract:
The Problem: Abscission is a highly regulated process, occurring as a natural terminal stage of development, in which various organs are separated from the parent plant. In most plant species, the process is initiated by a decrease in active auxin in the abscission zone (AZ) and an increase in ethylene, and may be accelerated by postharvest or environmental stresses. Another potential key regulator in abscission is IDA (Inflorescence Deficient in Abscission), which was identified as an essential peptide signal for floral organ abscission in Arabidopsis. However, information is still lacking regarding the molecular mechanisms integrating all these regulators. In our previous BARD funded research we made substantial progress towards understanding these molecular events in tomato, and the study is still in progress. We established a powerful platform for analysis of genes for regulatory proteins expressed in AZ. We identified changes in gene expression for several transcription factors (TFs) directly linked to ethylene and auxin signaling and several additional regulatory proteins not so obviously linked to these hormones. Moreover, we demonstrated using a virus-induced gene silencing (VIGS) assay that several play a functional role in the onset of abscission. Based on these results we have selected 14 genes for further analysis in stably transformed tomato plants. All 14 genes were suppressed by RNA interference (RNAi) using a constitutive promoter, and 5 of them were also suppressed using an abscission-specific promoter. Transformations are currently at different stages of progress including some lines that already display an abscission phenotype. Objectives: We propose here to (1) complete the functional analysis of the stably transformed tomato plants with T2 lines and perform transcriptome analysis using custom abscission-specific microarrays; (2) conduct an indepth analysis of the role of IDA signaling in tomato leaf and flower abscission; (3) perform transcriptome and proteome analyses to extend the earlier gene expression studies to identify transcripts and proteins that are highly specific to the separation layer (i.e., target cells for cell separation) prior to the onset of abscission; (4) extend and compliment the work in tomato using a winnowed set of genes in soybean. Methodology: Next Generation Sequencing (NGS) of mRNA will be used to further increase the list of abscission-associated genes, and for preparation of a custom tomato abscission microarray to test altered gene expression in transgenic plants. Tandem mass spectrometry (LC-MS/MS) of protein extracts from leaf petiole, flower pedicel and their AZ tissues will be used to identify the proteome of the AZ before and during abscission. AZ-specific gene promoters will be used in stably transformed tomato plants to reduce non-target phenotypes. The bean pod mottle virus (BPMV) plasmid vectors will be used for VIGS analysis in soybean. Expected Contribution: Our study will provide new insights into the regulation of ethylene-induced abscission by further revealing the role of key regulators in the process. This will permit development of novel techniques for manipulating leaf and flower abscission, thereby improving the postharvest performance of agriculturally important crops.