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1
Mallick, Sambit. "Book review: Asoke Basu and Saibal Datta, Indigenous Roots of Modern Science in Colonial Bengal: From Canon to Criticism (c. 1750–1950)." Sociological Bulletin 69, no. 2 (May 19, 2020): 297–99. http://dx.doi.org/10.1177/0038022920923246.
Abstract:
Asoke Basu and Saibal Datta, Indigenous Roots of Modern Science in Colonial Bengal: From Canon to Criticism (c. 1750–1950). Kolkata: The Asiatic Society, 2019, 294 pp., ₹650 (hardback). ISBN: 978-93-81574-82-9.
2
Jormanainen, Vesa, Maarit Rötsä, and Kimmo Parhiala. "Kuntien sosiaalitoimien sähköiset asiakastietojärjestelmät vuonna 2017." Finnish Journal of eHealth and eWelfare 11, no. 1-2 (March 10, 2019): 125–38. http://dx.doi.org/10.23996/fjhw.76835.
Abstract:
Tietotekniikasta on tullut keskeinen osa nykyisiä työympäristöjä, myös sosiaalihuollossa ja sosiaalityössä. Sosiaalihuollon atk-pohjaisia järjestelmiä oli jo 1990-luvun lopulla käytössä lähes kaikissa kunnissa. Aiemmin julkisen ja yksityisen sosiaalihuollon asiakastiedon tietojärjestelmien tuotemerkkejä on selvitetty organisaatiokyselyissä valtakunnallisesti vuosina 2001, 2011, 2014 ja 2017. Organisaatiokyselyjen ongelmana on ollut merkittävä (yli 50 %) vastaajakato. Selvitimme kuntien sosiaalitoimissa pääasiallisesti käytössä olleiden sähköisten asiakastietojärjestelmien tuotemerkit Suomen kaikissa kunnissa vuonna 2017. Erittäin keskittyneillä kuntien sosiaalitoimen sähköisten asiakastietojärjestelmien markkinoilla esiintyi 3 valmistajan 5 tuotemerkkiä 294 kunnassa vuonna 2017, kun sähköistä asiakastietojärjestelmää ei käytetty 17 kunnassa. Kuntien sosiaalitoimien sähköisten asiakastietojärjestelmien markkinoita hallitsivat Tieto ja CGI, joiden yhteenlaskettu markkinaosuus oli 90 % kuntien ja 95 % asukaslukujen perusteella. Tiedon Effica Sosiaalihuollon ja CGI:n Pro Consona Sosiaalitoimen markkinaosuudet yhteensä olivat 73–82 % kuntien tai asukaslukujen perusteella. Tuotemerkeistä ATJ/VATJ oli käytössä Helsingissä ja Vantaalla, joiden keskiväestö oli 427 000 (väestö 854 522 jaettuna kuntien lukumäärällä 2). Markkinajohtajista Effica oli käytössä suuremmissa (27 600 keskiväestö, 87 kuntaa) ja Pro Consona pienemmissä (9 600 keskiväestö, 170 kuntaa) kunnissa. Kuntien sosiaalitoimet käyttivät pääasiassa vain yhtä sosiaalihuollon sähköistä asiakastietojärjestelmää viiden, kahta 13:n ja 3–4:ää kolmen sairaanhoitopiirin alueella.
3
Chodosh, S., S. Lakshminarayan, H. Swarz, and S. Breisch. "Efficacy and Safety of a 10-Day Course of 400 or 600 Milligrams of Grepafloxacin Once Daily for Treatment of Acute Bacterial Exacerbations of Chronic Bronchitis: Comparison with a 10-Day Course of 500 Milligrams of Ciprofloxacin Twice Daily." Antimicrobial Agents and Chemotherapy 42, no. 1 (January 1, 1998): 114–20. http://dx.doi.org/10.1128/aac.42.1.114.
Abstract:
ABSTRACT A randomized, prospective, double-blind, double-dummy, multicenter study investigated the efficacy and safety of 10 days of oral therapy with grepafloxacin at 400 mg once daily, grepafloxacin at 600 mg once daily, or ciprofloxacin at 500 mg twice daily in 624 patients with acute bacterial exacerbations of chronic bronchitis. At the end of treatment, clinical success (cure or improvement) was achieved for 93% (140 of 151), 88% (137 of 156), and 91% (145 of 160) of patients in the groups receiving grepafloxacin at 400 mg, grepafloxacin at 600 mg, and ciprofloxacin, respectively (clinically evaluable population). At follow-up (14 to 28 days posttreatment), the clinical success rates were 87% (124 of 143), 81% (122 of 151), and 80% (123 of 154) in the groups receiving grepafloxacin at 400 mg and 600 mg and ciprofloxacin, respectively. A total of 379 pathogens were isolated from 290 patients, with the most common isolates being Moraxella catarrhalis(21%), Staphylococcus aureus (20%), Haemophilus influenzae (18%), and Streptococcus pneumoniae(7%). For the evaluable population, successful bacteriologic response was obtained at the end of treatment for 96% (92 of 96), 98% (87 of 89), and 92% (82 of 90) of patients receiving grepafloxacin at 400 mg, grepafloxacin at 600 mg, and ciprofloxacin, respectively, and was maintained in 86% (82 of 95), 88% (78 of 89), and 82% (69 of 84) of patients, respectively, at follow-up. All pretreatment S. pneumoniae isolates were susceptible to grepafloxacin, but two strains were resistant to ciprofloxacin. All treatments were well tolerated, with the most frequently reported drug-related adverse events being nausea, taste perversion, and headache. All drug-related adverse events in the grepafloxacin groups were mild or moderate in severity. This study demonstrates that 10-day courses of grepafloxacin given at 400 or 600 mg once daily were as effective, clinically and bacteriologically, as ciprofloxacin given at 500 mg twice daily for the treatment of acute bacterial exacerbations of chronic bronchitis.
4
Shah, Maunank, Sonia Paradis, Joshua Betz, Natalie Beylis, Renu Bharadwaj, Tatiana Caceres, Eduardo Gotuzzo, et al. "Multicenter Study of the Accuracy of the BD MAX Multidrug-resistant Tuberculosis Assay for Detection of Mycobacterium tuberculosis Complex and Mutations Associated With Resistance to Rifampin and Isoniazid." Clinical Infectious Diseases 71, no. 5 (September 27, 2019): 1161–67. http://dx.doi.org/10.1093/cid/ciz932.
Abstract:
Abstract Background Tuberculosis (TB) control is hindered by absence of rapid tests to identify Mycobacterium tuberculosis (MTB) and detect isoniazid (INH) and rifampin (RIF) resistance. We evaluated the accuracy of the BD MAX multidrug-resistant (MDR)-TB assay (BD MAX) in South Africa, Uganda, India, and Peru. Methods Outpatient adults with signs/symptoms of pulmonary TB were prospectively enrolled. Sputum smear microscopy and BD MAX were performed on a single raw sputum, which was then processed for culture and phenotypic drug susceptibility testing (DST), BD MAX, and Xpert MTB/RIF (Xpert). Results 1053 participants with presumptive TB were enrolled (47% female; 32% with human immunodeficiency virus). In patients with confirmed TB, BD MAX sensitivity was 93% (262/282 [95% CI, 89–95%]); specificity was 97% (593/610 [96–98%]) among participants with negative cultures on raw sputa. BD MAX sensitivity was 100% (175/175 [98–100%]) for smear-positive samples (fluorescence microscopy), and 81% (87/107 [73–88%]) in smear-negative samples. Among participants with both BD MAX and Xpert, sensitivity was 91% (249/274 [87–94%]) for BD MAX and 90% (246/274 [86–93%]) for Xpert on processed sputa. Sensitivity and specificity for RIF resistance compared with phenotypic DST were 90% (9/10 [60–98%]) and 95% (211/222 [91–97%]), respectively. Sensitivity and specificity for detection of INH resistance were 82% (22/27 [63–92%]) and 100% (205/205 [98–100%]), respectively. Conclusions The BD MAX MDR-TB assay had high sensitivity and specificity for detection of MTB and RIF and INH drug resistance and may be an important tool for rapid detection of TB and MDR-TB globally.
5
Alansari, Bader M. "INTERNAL CONSISTENCY OF AN ARABIC ADAPTATION OF THE BECK DEPRESSION INVENTORY-II WITH COLLEGE STUDENTS IN EIGHTEEN ARAB COUNTRIES." Social Behavior and Personality: an international journal 34, no. 4 (January 1, 2006): 425–30. http://dx.doi.org/10.2224/sbp.2006.34.4.425.
Abstract:
This study examined the adequacy and consistency of the second edition of the Beck Depression Inventory using an Arabic version of the BDI-II developed by Ghareeb (2000). Coefficient alphas were computed for samples of male and female undergraduates recruited from Palestine, Lebanon, Syria, Jordan, Saudi Arabia, Kuwait, Qatar, Bahrain, U.A. Emirates, Oman, Yemen, Egypt, Sudan, Tunisia, Libya, Algeria and Morocco (n = 600, 270, 479, 841, 943, 780, 781, 356, 230, 360, 333, 590, 735, 275, 291, 298, 300, 706, respectively). Values of alpha ranged between .82-.93. The inventory seems viable in the Arabic context, its use in cross-cultural research may be explored.
6
BRUMAR, DRAGOMIR, and MARIUS CIOBOATĂ. "GEOTECHNICAL DOCUMENTATION FOR THE CONSTRUCTION OF A GREENHOUSE IN THE LIMITROPHE AREA OF CRAIOVA." "Annals of the University of Craiova - Agriculture Montanology Cadastre Series " 51, no. 2 (December 20, 2020): 178–84. http://dx.doi.org/10.52846/aamc.2021.02.23.
Abstract:
The geotechnical documentation constitutes the quality verification at the requirement of resistance and stability of the foundation lands and earth masses and includes technical information about the land location and morphological data, classification in the geotechnical category, investigation of the foundation land, lithology and physical-mechanical characteristics of the land and the foundation conditions, obtained by performing a geotechnical drilling and dynamic penetration tests and the analysis of stratification, granulometry and physical-mechanical characteristics. The ground is flat and good foundation on 0.0-6.0 m depth, the conventional pressure is 170 kPa for Df = 0.8 m and B = 0.6 m and respectively 264 kPa for Df = 4.0 m and B = 2.0 m being recommended insulated foundations or insulated foundations with balancing beams, the terrain presents a low geotechnical risk and a degree of seismicity 82
7
Tian, Yanli, Yuqiang Zhao, Jiaju Zhou, Ting Sun, Xue Luo, Chet Kurowski, Weirong Gong, Baishi Hu, and Ron R. Walcott. "Prevalence of Acidovorax citrulli in Commercial Cucurbit Seedlots During 2010–2018 in China." Plant Disease 104, no. 1 (January 2020): 255–59. http://dx.doi.org/10.1094/pdis-03-19-0666-re.
Abstract:
Acidovorax citrulli is the causal agent of bacterial fruit blotch (BFB), a serious threat to cucurbit fruit and seed production worldwide. In recent years, the BFB has spread to many areas of China, mainly via the inadvertent distribution of contaminated commercial seeds. To assess the prevalence of seedborne A. citrulli in commercial watermelon and other cucurbitaceous seedlots in China, a 9-year survey was conducted between 2010 and 2018. A total of 4,839 seedlots of watermelon and other cucurbitaceous species were collected from 13 major seed production areas of China and tested by a semiselective media-based colony PCR technique for A. citrulli. Overall, A. citrulli was detected in 18.00% (871/4,839) of all cucurbitaceous seedlots. The bacterium was detected in 21.59% (38/176), 19.19% (33/172), 23.44% (214/913), 40.76% (247/606), 13.28% (85/640), 15.40% (95/617), 13.25% (73/551), 8.03% (48/598), and 6.71% (38/566) of all commercial seedlots tested from the 2010, 2011, 2012, 2013, 2014, 2015, 2016, 2017, and 2018 growing seasons, respectively. Additionally, the prevalence of A. citrulli in cucurbit seedlots was determined for different seed production areas. The prevalence of A. citrulli in cucurbitaceous seedlots produced in Xinjiang, Gansu, Ningxia, Inner Mongolia, and 9 other provinces was 18.76% (582/3103), 26.34% (103/391), 21.47% (82/382), 11.11% (14/126), and 10.75% (90/837), respectively. This is the first survey for A. citrulli in commercial cucurbit seeds in China, and the relatively high prevalence suggests that commercial seeds represent a substantial source of primary inoculum that can threaten cucurbit seed and fruit production in China.
8
JUNG, YANGJIN, ANNA C. S. PORTO-FETT, BRADLEY A. SHOYER, ELIZABETH HENRY, LAURA E. SHANE, MANUELA OSORIA, and JOHN B. LUCHANSKY. "Prevalence, Levels, and Viability of Salmonella in and on Raw Chicken Livers." Journal of Food Protection 82, no. 5 (April 22, 2019): 834–43. http://dx.doi.org/10.4315/0362-028x.jfp-18-430.
Abstract:
ABSTRACTWe surveyed chicken livers from various sources for the presence and levels of Salmonella. The pathogen was recovered from 148 (59.4%) of 249 chicken livers purchased at retail stores in Delaware, New Jersey, and Pennsylvania over about a 9-month period. Positive samples harbored Salmonella at levels of 6.4 most probable number (MPN)/g to 2.4 log CFU/g. The percentage of Salmonella-positive livers purchased at retail outlets in New Jersey (72%, 59 of 82 livers) was significantly higher (P < 0.05) than the percentage for livers purchased in Delaware (48%, 36 of 75 livers); however, this percentage was not significantly different (P > 0.05) from that for livers purchased in Pennsylvania (57.6%, 53 of 92 livers). The pathogen was also recovered more often (P = 0.019) from livers that were packaged by retailers (81 of 121 livers, 66.9%) than from livers packaged directly by processors (67 of 128 livers, 52.3%). In related studies, 12 (5.8%) of 207 chicken livers harvested from birds on a research farm tested positive for Salmonella at levels of 0.4 to 2.2 MPN/g. The recovery rate of Salmonella was 4.4% (6 of 135 livers) from livers with the gall bladder attached and 8.3% (6 of 72 livers) from livers when the gall bladder was removed at harvest on the research farm. We also quantified the levels of a nine-strain cocktail (ca. 6.5 log CFU/g) of Salmonella strains inoculated externally onto or internally into livers both before and after extended cold storage. Storage for at least 2 days at 4°C or 15 days at −20°C resulted in a decrease of about 1.0 log CFU/g in pathogen levels. Given the relatively high recovery rate (ca. 6.0 to 60.0%) and high (possibly illness causing) levels (0.4 MPN/g to 2.4 CFU/g) of Salmonella associated with chicken livers in the present study, further interventions for processors are needed to lower the prevalence and levels of this pathogen on poultry liver.
9
Khan, Huma, Saurabh Mishra, Dhirendra Kumar Srivastava, and Reena Srivastava. "An assessment of the awareness and practices regarding HIV/AIDS among married women of the reproductive age group in urban slums of Eastern Uttar Pradesh." International Journal Of Community Medicine And Public Health 6, no. 8 (July 26, 2019): 3496. http://dx.doi.org/10.18203/2394-6040.ijcmph20193478.
Abstract:
Background: HIV/AIDS is such a dreaded disease which can be prevented if the people are aware of the ways to do so. Urban slums share a specific position in the demographic profile of district because of the inequalities of health care system where the rural health care system is far to reach and the more flourished private health care system is unaffordable for most of the inhabitants. HIV/AIDS is not only a health-related problem but it also has socioeconomic, behavioral and cultural aspects.Methods: Descriptive cross-sectional among currently married women in reproductive age group (15-49 years) residing in any of the selected urban slums of district Gorakhpur. Cluster sampling was used and clusters were chosen by probability proportional to size (PPS) method of cluster sampling from a list of urban slums obtained from District Urban Development Authority (DUDA), Gorakhpur. The final sample size was 600.Results: 82% of women had ever heard about HIV/AIDS and 72% of women got the knowledge about HIV through television followed by 56% of woman who got knowledge by relatives and friends. 68% of woman believes that the mode of transmission of HIV is unsafe sex followed by 58% of women who believed that infected blood transfusion is the mode of transmission of HIV.Conclusions: Awareness is crucial in the prevention and control of HIV/AIDS. Women should have more access to the knowledge resources and facilities for testing and prevention of HIV/AIDS.
10
Jontof-Hutter, Daniel, Paul A. Dalba, and John H. Livingston. "TESS Observations of Kepler Systems with Transit Timing Variations." Astronomical Journal 164, no. 2 (July 7, 2022): 42. http://dx.doi.org/10.3847/1538-3881/ac7396.
Abstract:
Abstract We identify targets in the Kepler field that may be characterized by transit timing variations and are detectable by the Transiting Exoplanet Survey Satellite (TESS). Despite the reduced signal-to-noise ratio of TESS transits compared to Kepler, we recover 48 transits from 13 systems in Sectors 14, 15, 26, 40 and 41. We find strong evidence of a nontransiting perturber orbiting Kepler-396 (KOI-2672) and explore two possible cases of a third planet in that system that could explain the measured transit times. We update the ephemerides and mass constraints where possible at KOI-70 (Kepler-20), KOI-82 (Kepler-102), KOI-94 (Kepler-89), KOI-137 (Kepler-18), KOI-244 (Kepler-25), KOI-245 (Kepler-37), KOI-282 (Kepler-130), KOI-377 (Kepler-9), KOI-620 (Kepler-51), KOI-806 (Kepler-30), KOI-1353 (Kepler-289), and KOI-1783 (Kepler-1662).
11
Wise, R., T. Gee, G. Marshall, and J. M. Andrews. "Single-Dose Pharmacokinetics and Penetration of BMS 284756 into an Inflammatory Exudate." Antimicrobial Agents and Chemotherapy 46, no. 1 (January 2002): 242–44. http://dx.doi.org/10.1128/aac.46.1.242-244.2002.
Abstract:
ABSTRACT The pharmacokinetics of a single dose of BMS 284756 were determined following oral administration of a 600-mg dose to eight healthy male volunteers. Concentrations of the drug were measured in plasma and a cantharidine-induced inflammatory exudate by a microbiological assay. The mean peak concentration in plasma of 10.4 μg/ml (standard deviation [SD], 1.3 μg/ml) was attained at a mean time of 1.2 h (SD, 0.5 h) after the dose. The penetration into the inflammatory exudate was 82% (SD, 15.7%). A mean peak concentration of 7.2 μg/ml (SD, 2.4 μg/ml) was attained in the inflammatory exudate at 5.3 h (SD, 1.5 h). The elimination half-lives from plasma and inflammatory fluid were 9.8 h (SD, 1.1 h) and 8.5 h (SD, 1.9 h), respectively. The areas under the concentration-time curves for plasma and inflammatory fluid were 96.7 μg · h/ml (SD, 10.3 μg · h/ml) and 77.9 μg · h/ml (SD, 19.2 μg · h/ml), respectively.
12
Matos, Fernando B., Amabily Bohn, and Paulo H. Labiak. "The ferns and lycophytes of Reserva Natural Guaricica, Antonina, Paraná, Brazil." Check List 16, no. 1 (February 21, 2020): 183–206. http://dx.doi.org/10.15560/16.1.183.
Abstract:
We found 204 species of pteridophytes in Reserva Natural Guaricica, a private natural heritage reserve (RPPN) in Antonina, Paraná, Brazil. With approximately 8,600 ha and elevations ranging from sea level to 600 m, RPPN Guaricica has more species of pteridophytes than any other area in Paraná. Ferns are represented by 194 species in 82 genera and 26 families, whereas lycophytes comprise 10 species in four genera and two families. The RPPN is the type locality of two recently described species: Hypolepis acantha Schwartsb. and Oleandra australis Schwartsb. & J.Prado. It is also the only place of occurrence of Didymoglossum angustifrons Fée, Diplazium riedelianum (Bong. ex Kuhn) C.Chr., Pteris ensiformis Burm.f., P. tripartita Sw., Saccoloma elegans Kaulf., and Steiropteris polypodioides (Raddi) Salino & T.E.Almeida in Paraná. Pteris ensiformis and Saccoloma brasiliense (C.Presl) Mett. are new state records. Additional species are expected to occur in the area, in view of their known geographical ranges.
13
Bradac, J. A., C. E. Gruber, S. Forry-Schaudies, and S. H. Hughes. "Isolation and characterization of related cDNA clones encoding skeletal muscle beta-tropomyosin and a low-molecular-weight nonmuscle tropomyosin isoform." Molecular and Cellular Biology 9, no. 1 (January 1989): 185–92. http://dx.doi.org/10.1128/mcb.9.1.185-192.1989.
Abstract:
We have isolated and characterized cDNA clones from chicken cDNA libraries derived from skeletal muscle, body wall, and cultured fibroblasts. A clone isolated from a skeletal muscle cDNA library contains the complete protein-coding sequence of the 284-amino-acid skeletal muscle beta-tropomyosin together with 72 bases of 5' untranslated sequence and nearly the entire 3' untranslated region (about 660 bases), lacking only the last 4 bases and the poly(A) tail. A second clone, isolated from the fibroblast cDNA library, contains the complete protein-coding sequence of a 248-amino-acid fibroblast tropomyosin together with 77 bases of 5' untranslated sequence and 235 bases of 3' untranslated sequence through the poly(A) tract. The derived amino acid sequence from this clone exhibits only 82% homology with rat fibroblast tropomyosin 4 and 80% homology with human fibroblast tropomyosin TM30nm, indicating that this clone encodes a third 248-amino-acid tropomyosin isoform class. The protein product of this mRNA is fibroblast tropomyosin 3b, one of two low-molecular-weight isoforms expressed in chicken fibroblast cultures. Comparing the sequences of the skeletal muscle and fibroblast cDNAs with a previously characterized clone which encodes the smooth muscle alpha-tropomyosin reveals two regions of absolute homology, suggesting that these three clones were derived from the same gene by alternative RNA splicing.
14
El Mokni, Ridha, Giulio Barone, Nigel Maxted, Shelagh Kell, and Gianniantonio Domina. "A prioritised inventory of crop wild relatives and wild harvested plants of Tunisia." Genetic Resources and Crop Evolution 69, no. 5 (January 19, 2022): 1787–816. http://dx.doi.org/10.1007/s10722-021-01340-z.
Abstract:
AbstractAn inventory of crop wild relatives (CWR) and wild harvested plants (WHP) occurring in Tunisia, based on the integration of the last available floristic checklists, is presented. The taxa were prioritised according to economic value of the related crop, potential for crop improvement, threat status, endemism, inclusion in the ITPGRFA (Annex I) and average annual contributions to dietary energy (kilocalories) per capita per day by applying a scoring system based on 4 priority levels. Of a total of 2912 taxa belonging to the Tunisian Flora, 2504 CWR and/or WHP (86% of the total), from 143 families and 686 genera, were identified, 2445 of which are CWR and 847 are WHP. In detail, 1654 are solely CWR and 59 are WHP only, whereas 788 are both CWR and WHP. The final priority list for active conservation includes 1036 CWR (43% of the total CWR taxa), with 139 taxa rated as high priority, 660 medium priority and 237 low priority. The final priority list for WHP is composed of 344 taxa and includes eight high priority, 254 medium priority and 82 low priority taxa. Our results confirm Tunisia as a hotspot of CWR and WHP diversity in the Mediterranean area. The inventory here proposed provides the basis for the development and implementation of a more targeted national CWR/WHP conservation strategy for Tunisia.
15
Bradac, J. A., C. E. Gruber, S. Forry-Schaudies, and S. H. Hughes. "Isolation and characterization of related cDNA clones encoding skeletal muscle beta-tropomyosin and a low-molecular-weight nonmuscle tropomyosin isoform." Molecular and Cellular Biology 9, no. 1 (January 1989): 185–92. http://dx.doi.org/10.1128/mcb.9.1.185.
Abstract:
We have isolated and characterized cDNA clones from chicken cDNA libraries derived from skeletal muscle, body wall, and cultured fibroblasts. A clone isolated from a skeletal muscle cDNA library contains the complete protein-coding sequence of the 284-amino-acid skeletal muscle beta-tropomyosin together with 72 bases of 5' untranslated sequence and nearly the entire 3' untranslated region (about 660 bases), lacking only the last 4 bases and the poly(A) tail. A second clone, isolated from the fibroblast cDNA library, contains the complete protein-coding sequence of a 248-amino-acid fibroblast tropomyosin together with 77 bases of 5' untranslated sequence and 235 bases of 3' untranslated sequence through the poly(A) tract. The derived amino acid sequence from this clone exhibits only 82% homology with rat fibroblast tropomyosin 4 and 80% homology with human fibroblast tropomyosin TM30nm, indicating that this clone encodes a third 248-amino-acid tropomyosin isoform class. The protein product of this mRNA is fibroblast tropomyosin 3b, one of two low-molecular-weight isoforms expressed in chicken fibroblast cultures. Comparing the sequences of the skeletal muscle and fibroblast cDNAs with a previously characterized clone which encodes the smooth muscle alpha-tropomyosin reveals two regions of absolute homology, suggesting that these three clones were derived from the same gene by alternative RNA splicing.
16
Gómez-Méndez, Luis D., Luis C. Jiménez-Borrego, Alejandro Pérez-Flórez, Raúl A. Poutou-Piñales, Aura M. Pedroza-Rodríguez, Juan C. Salcedo-Reyes, Andrés Vargas, and Johan M. Bogoya. "LDPE Transformation by Exposure to Sequential Low-Pressure Plasma and TiO2/UV Photocatalysis." Molecules 26, no. 9 (April 26, 2021): 2513. http://dx.doi.org/10.3390/molecules26092513.
Abstract:
Low-density polyethylene (LDPE) sheets (3.0 ± 0.1 cm) received sequential treatment, first by the action of direct-current low-pressure plasma (DC-LPP) with a 100% oxygen partial pressure, 3.0 × 10−2 mbar pressure, 600 V DC tension, 5.6 cm distance, 6-min treatment. Then, sheets were submitted to TiO2 photocatalysis at UV radiation at 254 nm (TiO2/UV) with a pH value of 4.5 ± 0.2 and a TiO2 concentration of 1 gL−1. We achieved a complementary effect on the transformation of LDPE films. With the first treatment, ablation was generated, which increased hydrophilicity. With the second treatment, the cavities appeared. The changes in the LDPE sheets’ hydrophobicity were measured using the static contact angle (SCA) technique. The photocatalytic degradation curve at 400 h revealed that the DC-LPP photocatalysis sequential process decreased SCA by 82°. This was achieved by the incorporation of polar groups, which increased hydrophilicity, roughness, and rigidity by 12 and 38%, respectively. These sequential processes could be employed for LDPE and other material biodegradation pretreatment.
17
Mauad, Edmundo Carvalho, Uilho Antonio Gomes, Domingos Boldrini, Raphael Haikel, Geraldo Hidalgo Santiago, and Luiz M. Maruta. "Câncer de Cólon e Reto em Pacientes Abaixo dos 40 Anos." Revista Brasileira de Cancerologia 46, no. 4 (December 29, 2000): 371–76. http://dx.doi.org/10.32635/2176-9745.rbc.2000v46n4.2421.
Abstract:
O objetivo deste trabalho foi avaliar os aspectos clínicos e sobrevida dos pacientes com câncer colorretal com idade inferior a 40 anos de idade. No período de 1968 a 1996 foram estudados 90 pacientes neste grupo etário na Fundação Pio XII – Hospital São Judas Tadeu de Barretos. Os prontuários médicos constituíram a base dessa análise. Houve comprovação histopatológica em todos esses pacientes. Para análise estatística utilizou-se o programa NCSS 6.0. Em relação ao sexo 51% eram masculino e 32 anos foi a idade média encontrada. Os sintomas mais comuns foram sangramento retal, dor perineal ou abdominal. A localização topográfica mais freqüente foi o reto com quase metade dos pacientes. Somente dois (2.4%) dos 82 pacientes apresentaram câncer no estadiamento inicial. A sobrevida média foi de 39 meses (variando de 1 a 334 meses). Houve menor sobrevida dos pacientes em estádio clínico (EC) III e IV, em comparação com os em EC II (P < 0.005). Concluímos que apesar deste trabalho ter sido feito com base em prontuários médicos, a proporção de casos de câncer nesta idade foi maior do que a apontada pela maioria dos outros trabalhos e merece melhor estudo.
18
Сандухадзе, Баграт Исменович, Людмила Александровна Марченкова, Рамин Закирович Мамедов, Ольга Викторовна Павлова, Валентина Васильевна Бугрова, Мария Сергеевна Крахмалёва, Раиса Федоровна Чавдарь, Татьяна Григорьевна Орлова, and Ярослав Станиславович Молодовский. "Характеристика сортов и линий озимой пшеницы по морфофизиологическим параметрам." Аграрная Россия, no. 4 (April 22, 2023): 15–20. http://dx.doi.org/10.30906/1999-5636-2023-4-15-20.
Abstract:
Представлены экспериментальные данные по оценке морфофизиологических показателей проростков перспективных линий озимой пшеницы в сравнении с контрольными сортами Московская 39 (стандарт) и Московская 40 на начальных этапах их роста и развития. Изучаемые генотипы характеризуются высокой урожайностью в пределах 6,4 – 7,2 т/га, массой 1000 семян — 41,6 – 45,4 г, массой зерна с колоса — 1,13 – 1,86 г и высотой — 82 – 115 см. Установлен широкий полиморфизм органов растений, показана зависимость активизации ростовых процессов от сортовых особенностей. Определен уровень внутрисортового варьирования ростовых функций (6,0 – 17,7 %) и отмечено, что наименьшей средней вариацией отличается длина корешков (7,7 %), наибольшей — ростков (11,3 %). Выявлена степень корреляционной сопряженности всех изучаемых параметров и показана зависимость урожайности и семенной продуктивности от степени развития органов проростков на начальных этапах их роста (r = 0,52 – 0,95). Установлено, что на ранних этапах органогенеза преимущество имеют образцы с высокой активизацией роста ростков и корешков. На основе суммы числовых показателей адаптивности, морфофизиологических параметров, урожайности и элементов семенной продуктивности, преобразованных в ранговый формат, определена биологическая полноценность семян сортов и проростков озимой пшеницы и выявлены сорта и линии, формирующие наиболее широкий диапазон положительных признаков и характеризующиеся самым высоким рейтингом, среди которых Эритроспермум 2294/12, Эритроспермум 383/20 и Эритроспермум 69/21.
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Hakimi, A. Ari, Yasser Ged, Jessica Flynn, Douglas R. Hoen, Renzo G. Di Natale, Kyle A. Blum, Vladimir Makarov, et al. "The impact of PBRM1 mutations on overall survival in greater than 2,100 patients treated with immune checkpoint blockade (ICB)." Journal of Clinical Oncology 37, no. 7_suppl (March 1, 2019): 666. http://dx.doi.org/10.1200/jco.2019.37.7_suppl.666.
Abstract:
666 Background: PBRM1 is the second most commonly mutated gene in clear cell renal cell carcinoma (ccRCC). We have previously shown favorable outcomes in PBRM1-mutated ccRCC tumors treated with vascular endothelial growth factor (VEGF) inhibitors. Recent data suggested PBRM1 mutations may sensitize ccRCC and non RCC malignancies to ICB therapy. We queried the impact of PBRM1 loss on overall survival (OS) across 2,152 patients treated with ICB. Methods: PBRM1 mutations were assessed in metastatic ccRCC patients who received first line (n = 82) or second line (n = 61) ICB or ICB/VEGF combinations. Additionally, 41 cohorts of non-RCC malignancies treated with ICB and combination (n = 2,009) were analyzed. Mutations were assessed by next generation targeted sequencing using archival tissue. Association of mutation status and overall survival (OS) was tested by multivariate Cox regression analysis (MVA) and adjusted for tumor mutation burden (TMB), copy number alterations (CNA), loss of function(LOF) mutations (non RCC cohort) and IMDC risk (for ccRCC patients). Results: PBRM1 mutations were not associated with improved OS in ICB the entire ccRCC cohort (HR 1.37; CI 0.79-2.4; p = 0.265), the first line (p = 0.624) or second line setting (p = 0.39) or as combination with VEGF inhibitors (p = 0.2). Several RCC subgroups were investigated (see Table at bottom). In the non-RCC cohorts (n = 2,009) PBRM1 mutations were not significantly associated with OS on univariate analysis (HR = 0.73, p = 0.22 for LOF and HR = 0.84,p = 0.34 for non LOF), and remained insignificant after adjusting for TMB, total CNA, and drug class (CTLA4, PD-1/PDL-1 and combinations) (HR = 1.07, p = 0.78 for LOF and HR = 1.08,p = 0.67 for non LOF). Conclusions: Neither in ccRCC nor in the pan-cancer cohort did PBRM1 mutations appear to be associated with improved overall survival with ICB therapy.[Table: see text]
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Halai, Mansur M., Ellie Pinsker, and Timothy R. Daniels. "Effect of Novel Anteromedial Approach on Wound Complications Following Ankle Arthroplasty." Foot & Ankle International 41, no. 10 (July 18, 2020): 1198–205. http://dx.doi.org/10.1177/1071100720937247.
Abstract:
Background: The purpose of this study was to evaluate all wound complications following total ankle arthroplasty (TAA) before and after the implementation of a novel extensile anteromedial surgical approach. We further investigated patients and operative factors associated with wound complications. Methods: A series of 660 TAAs were collected. The median follow-up was 84 months (range, 12-204 months). Minor wound complications included discoloration, swelling, or clinical concern that merited bringing the patient back sooner for review. Major wound complications included plastic surgical consultation, debridement by the nurse specialist, negative pressure therapy, or reoperation. All major complications initially had a course of empirical oral antibiotics. Since 2011, higher-risk patients have had their TAA performed through an extensile anteromedial approach. Results: Fifty-six patients (8.5%) had wound healing issues (17 major [2.6%], 39 minor [5.9%]). All issues became evident <21 days postoperatively. Inflammatory arthritis, smoking, and coronary disease were patient factors associated with major wound complications. Four patients were treated with negative pressure therapy, 4 with debridement in the operating room, and 2 eventually converted to fusions. Fourteen patients (82%) who had major complications had an ancillary procedure to balance the TAA through a separate incision. All major wound complications had an anterior surgical approach for their TAA. Before 2011, there were 13 major wound complications. Since 2012, there have been 4 major wound complications overall, with no wound complications in the anteromedial approach cohort. Conclusion: We recommend an extensile anteromedial approach for smokers and patients with heart disease or inflammatory arthritis undergoing TAA. If a wound complication developed, a multidisciplinary team to evaluate wounds was useful. Level of Evidence: Level III, retrospective comparative series.
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Miller, Janette Brand, Stefan Bull, John Miller, and Patricia McVeagh. "The Oligosaccharide Composition of Human Milk." Journal of Pediatric Gastroenterology and Nutrition 19, no. 4 (November 1994): 371–76. http://dx.doi.org/10.1002/j.1536-4801.1994.tb11454.x.
Abstract:
SummaryOligosaccharides are an important component of human milk, but little is known about variations in their composition. The aim of this study was to determine the temporal and inter‐individual variations in carbohydrate composition of human milk during the first 3 months of lactation. Serial milk samples of 10 mothers (eight full‐term and two preterm births) were analyzed to determine the concentration of lactose and three monosaccharide components derived from the non‐lactose carbohydrate: sialic acid, N‐acetylglucosamine, and fucose. In full‐term milk, sialic acid and N‐acetylglucosamine were found to decrease significantly (p < 0.05) from weeks 1 to 13 postnatally. On average (mean ± SD), sialic acid decreased by 71% (from 879 ± 157 to 256 ± 82 mg/L; p < 0.05) and N‐acetylglucosamine by 56% (from 1,459 ± 282 to 646 ± 214 mg/L; p < 0.05), while fucose decreased by only 35% (from 660 ± 192 to 432 ± 180 mg/L; p > 0.05). On average, lactose concentration increased by 17% over the same period, from 55.4 ± 4.2 g/L in week 1 to 64.9 ± 2.3 g/L at 3 months. Preterm milk contained higher concentrations of each component, but temporal changes were similar to those seen in full‐term milk. Apart from temporal changes, there were large inter‐individual differences in oligosaccharide composition: fucose varied fourfold, sialic acid threefold, and N‐acetylglucosamine twofold among women at the same stage of lactation. The changes observed may simply reflect the aging of the cells responsible for milk secretion, but they are also consistent with a programmed adaptation of the milk composition to the needs of the infant.
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Kedem, J., C. Wright, P. M. Scholz, and H. R. Weiss. "Effect of dopamine on local segment work and O2 consumption in collateral-dependent myocardium." American Journal of Physiology-Heart and Circulatory Physiology 267, no. 1 (July 1, 1994): H17—H23. http://dx.doi.org/10.1152/ajpheart.1994.267.1.h17.
Abstract:
To test the hypothesis that dopamine-stimulated work and myocardial O2 consumption (MVO2) in collateral-dependent myocardium would be reduced, Ameroid constrictors were implanted around the circumflex coronary artery (CFX) in nine dogs. Four weeks later, in an anesthetized open-chest preparation, segment length (ultrasonic dimension gauge) and force (miniature force transducer) were measured in myocardium supplied by the CFX and left anterior descending (LAD) coronary arteries. Work in each region was calculated as the systolic area under the force-length curve. Corresponding regional MVO2 was calculated from local O2 extraction (microspectrophotometry) and flow (radiolabeled microspheres). Dopamine infusion (15 micrograms.kg-1.min-1) increased regional work from 262 +/- 56 to 733 +/- 171 mm.g.min-1 in the control (LAD) region, but to a much smaller extent in the collateral-dependent (CFX) region (from 249 +/- 82 to 414 +/- 81 mm.g.min-1). However, regional MVO2 increased to about the same extent in the CFX (from 6.0 +/- 0.7 to 12.4 +/- 0.9 ml O2.min-1 times 100 g-1) and the LAD region (from 7.0 +/- 0.6 to 14.5 +/- 1.3 ml O2.min-1 times 100 g-1). O2 extraction was not elevated in the CFX region. Therefore, the functional impairment was not secondary to O2 supply or consumption limitations.
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Strange, Geoffrey A., Simon Stewart, Nick Curzen, Simon Ray, Simon Kendall, Peter Braidley, Keith Pearce, Renzo Pessotto, David Playford, and Huon H. Gray. "Uncovering the treatable burden of severe aortic stenosis in the UK." Open Heart 9, no. 1 (January 2022): e001783. http://dx.doi.org/10.1136/openhrt-2021-001783.
Abstract:
ObjectiveTo estimate the population prevalence and treatable burden of severe aortic stenosis (AS) in the UK.MethodsWe adapted a contemporary model of the population profile of symptomatic and asymptomatic severe AS in Europe and North America to estimate the number of people aged ≥55 years in the UK who might benefit from surgical aortic valve replacement (SAVR) or transcatheter aortic valve implantation (TAVI).ResultsWith a point prevalence of 1.48%, we estimate that 291 448 men and women aged ≥55 years in the UK had severe AS in 2019. Of these, 68.3% (199 059, 95% CI 1 77 201 to 221 355 people) would have been symptomatic and, therefore, more readily treated according to their surgical risk profile; the remaining 31.7% of cases (92 389, 95% CI 70 093 to 144 247) being asymptomatic. Based on historical patterns of intervention, 58.4% (116 251, 95% CI 106 895 to 1 25 606) of the 199 059 symptomatic cases would qualify for SAVR, with 7208 (95% CI 7091 to 7234) being assessed as being in a high, preoperative surgical risk category. Among the remaining 41.6% (82 809, 95% CI 73 453 to 92 164) of cases potentially unsuitable for SAVR, an estimated 61.7% (51 093, 95% CI 34 780 to 67 655) might be suitable for TAVI. We estimate that 172 859 out of 291 448 prevalent cases of severe AS (59.3%) will subsequently die within 5 years without proactive management.ConclusionsThese data suggest a high burden of severe AS in the UK requiring surgical or transcatheter intervention that challenges the ongoing capacity of the National Health Service to meet the needs of those affected.
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Gutiérrez-Cázarez, Zita, Firdausi Qadri, M. John Albert, and Jorge A. Girón. "Identification of Enterotoxigenic Escherichia coli Harboring Longus Type IV Pilus Gene by DNA Amplification." Journal of Clinical Microbiology 38, no. 5 (2000): 1767–71. http://dx.doi.org/10.1128/jcm.38.5.1767-1771.2000.
Abstract:
DNA amplification of lngA, the structural gene of longus type IV pilus produced by human enterotoxigenicEscherichia coli (ETEC) was achieved by the use of specific oligonucleotide primers designed from the nucleotide sequence oflngA. A 630-bp fragment representing the entirelngA gene was amplified in eight prototype strains previously characterized as longus positive. Five ETEC strains producing colonization factor antigen III (CFA III) (also a type IV pilus) were also positive by PCR, confirming the DNA homology between CFA III and longus. None of the non-ETEC and non-E. colienteropathogens studied showed the 0.63-kbp amplicon. The procedure thus detected only ETEC strains harboring type IV pili genes with or without other colonization factors. Except for five lngAPCR-positive, probe-positive strains, all lngA PCR-positive strains produced the pilin as demonstrated by immunoblotting. To test the amplification procedure in a clinical setting, a collection of 264 fresh clinical E. coli strains isolated from 88 Mexican children with diarrhea was screened by PCR. Among 82 ETEC isolates found, 30 (36.5%) were lngA PCR-positive. Twenty-seven percent of the children shed ETEC that possessed lngA. In parallel with DNA probes or PCR protocols to detect enterotoxin genes, the lngA PCR method may prove useful for detection of ETEC harboring type IV pilus genes in epidemiological studies.
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Mortley, D. G., P. A. Loretan, W. A. Hill, C. K. Bonsi, C. E. Morris, R. Hall, and D. Sullen. "Biocompatibility of Sweetpotato and Peanut in a Hydroponic System." HortScience 33, no. 7 (December 1998): 1147–49. http://dx.doi.org/10.21273/hortsci.33.7.1147.
Abstract:
`Georgia Red' peanut (Arachis hypogaea L.) and TU-82-155 sweetpotato [Ipomoea batatas (L.) Lam] were grown in monocultured or intercropped recirculating hydroponic systems in a greenhouse using the nutrient film technique (NFT). The objective was to determine whether growth and subsequent yield would be affected by intercropping. Treatments were sweetpotato monoculture (SP), peanut monoculture (PN), and sweetpotato and peanut grown in separate NFT channels but sharing a common nutrient solution (SP-PN). Greenhouse conditions ranged from 24 to 33 °C, 60% to 90% relative humidity (RH), and photosynthetic photon flux (PPF) of 200 to 1700 μmol·m-2·s-1. Sweetpotato cuttings (15 cm long) and 14-day-old seedlings of peanuts were planted into growth channels (0.15 × 0.15 × 1.2 m). Plants were spaced 25 cm apart within and 25 cm apart between growing channels. A modified half-Hoagland solution with a 1 N : 2.4 K ratio was used. Solution pH was maintained between 5.5 and 6.0 for treatments involving SP and 6.4 and 6.7 for PN. Electrical conductivity (EC) ranged between 1100 and 1200 μS·cm-1. The number of storage roots per sweetpotato plant was similar for both SP and SP-PN. Storage root fresh and dry mass were 29% and 36% greater, respectively, for plants in the SP-PN treatment than for plants in the SP treatment. The percent dry mass of the storage roots, dry mass of fibrous and pencil roots, and the length-to-diameter ratio of storage roots were similar for SP and SP-PN sweetpotato plants. Likewise, foliage fresh and dry mass and harvest index were not significantly influenced by treatment. Total dry mass was 37% greater for PN than for SP-PN peanut plants, and pod dry mass was 82% higher. Mature and total seed dry mass and fibrous root dry mass were significantly greater for PN than for SP-PN plants. Harvest index (HI) was similar for both treatments. Root length tended to be lower for seedlings grown in the nutrient solution from the SP-PN treatment.
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Ademi, A., A. Bernhoft, E. Govasmark, H. Bytyqi, T. Sivertsen, and B. R. Singh. "Selenium and other mineral concentrations in feed and sheep's blood in Kosovo." Translational Animal Science 1, no. 1 (February 1, 2017): 97–107. http://dx.doi.org/10.2527/tas2016.0010.
Abstract:
Abstract The aim of this study was to assess the concentration of Se and other minerals in sheep and the supplied feed. Four macrominerals (Ca, P, Mg, and S), 7 microminerals (Se, Fe, Zn, Cu, Mn, Co, and Mo), and 2 toxic minerals (Cd and Pb) were analyzed in 69 feed and 292 sheep blood samples from 30 farms in different regions of Kosovo. The samples were analyzed using inductively coupled plasma mass spectrometry, and mineral concentrations in whole blood were measured to assess their status in animals. Concentrations of the different minerals in feed were found in the following ranges: 1.9 to 9.5 g Ca/kg DM, 0.8 to 3.2 g P/kg DM, 0.8 to 3.2 g Mg/kg DM, 1.0 to 2.8 g S/kg DM, 6 to 82 µg Se/kg DM, 33 to 970 mg Fe/kg DM, 15 to 42 mg Zn/ kg DM, 2.6 to 7.5 mg Cu/kg DM, 26 to 250 mg Mn/kg DM, 0.04 to 0.88 mg Co/kg DM, 0.05 to 0.86 mg Mo/ kg DM, 0.07 to 2.02 mg Pb/kg DM, and 0.02 to 0.19 mg Cd/kg DM. Concentrations of the microminerals analyzed in whole blood were found in the following ranges: 15 to 360 µg Se/L, 190 to 500 mg Fe/L, 1.4 to 3.8 mg Zn/L, 0.3 to 2.6 mg Cu/L, 6 to 243 µg Mn/L, 0.1 to 19.6 µg Co/L, and 1.8 to 66.0 µg Pb/L. Among all minerals, the largest deficiency was found for Se both in feed and sheep blood, with 82% of feed samples and 83% blood samples being inadequate, and its supplementation is necessary. Selenium-supplemented sheep had significantly higher Se concentration in blood than non-supplemented sheep (P < 0.01). In addition, other macro- and microminerals in feed such as P, S, Cu, and Co were at inadequate concentrations at some of the farms, and supplementation may also be needed for these minerals.
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Olisov, O. D., M. S. Novruzbekov, V. A. Gulyaev, and K. N. Lutsyk. "The role of calcineurin inhibitors in the progression of hepatocellular carcinoma after liver transplantation." Transplantologiya. The Russian Journal of Transplantation 14, no. 3 (September 15, 2022): 292–300. http://dx.doi.org/10.23873/2074-0506-2022-14-3-292-300.
Abstract:
Introduction. Orthotopic liver transplantation is the most radical method of treatment of hepatocellular carcinoma. The high recurrence rate limits the use of transplantation in patients with hepatocellular cancer. Immunosuppressive therapy may affect the frequency of oncoprogression after liver transplantationAim. To evaluate the role of immunosuppressive therapy in the postoperative progression of hepatocellular cancer in patients after liver transplantationMaterial and methods. The recurrence rate of hepatocellular cancer and tumor free survival in 104 patients after liver transplantation were analyzed. To evaluate the effect of the immunosuppression main component concentration on the postoperative progression of hepatocellular carcinoma, we studied the mean baseline concentration (C0) for the entire follow-up period for patients with a tumor-free period and the mean baseline concentration for patients with hepatocellular carcinoma progression, in whom only the duration of the tumor-free period was studied. According to the degree of tumor lesion, patients were distributed in accordance with the Milan criteria (based on the results of a pathologic and morphological examination of the recipient's explanted liver.Results. The values of the baseline blood level of tacrolimus>6.0 ng/ml and cyclosporine A>100 ng/ml is associated with a high rate of progression of hepatocellular cancer. Reducing the load of calcineurin inhibitors can reduce the incidence of cancer progression by at least 2 times. The values of 1-, 3- and 5-year relapse-free survival in patients with advanced cancer and low figures of the baseline blood level of calcineurin inhibitor are 82%, 70% and 70%, respectively.Conclusion. Minimization of immunosuppression is of crucial importance in the prevention of posttransplant progression of hepatocellular cancer, especially among patients with its common form.
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Sasaki, Koji, Ildefonso Ismael Rodriguez-Rivera, Hagop M. Kantarjian, Susan O'Brien, Elias Jabbour, Gautam Borthakur, Farhad Ravandi, Michael J. Burke, Patrick A. Zweidler-McKay, and Jorge E. Cortes. "Correlation of Lymphocyte Count with Treatment Response to Tyrosine Kinase Inhibitors in Newly Diagnosed Chronic Myeloid Leukemia in Chronic Phase." Blood 124, no. 21 (December 6, 2014): 4538. http://dx.doi.org/10.1182/blood.v124.21.4538.4538.
Abstract:
Abstract Background: Total lymphocyte count (TLC) has been shown to correlate with outcomes in patients (pts) with acute leukemia. The clinical correlation to TLC in pts with chronic myeloid leukemia in chronic phase (CML-CP) who were treated with a tyrosine-kinase inhibitor (TKI) is unclear. Methods: Lymphocyte data in pts with newly diagnosed CML-CP who were enrolled in consecutive or parallel clinical trials with front-line imatinib (IM), nilotinib (Nilo), or dasatinib (Dasa) were collected at the time of diagnosis, and 3 and 6 months (M) after the start of TKI. Relative lymphocytrosis (RLC) was defined as lymphocyte >150% at 3 or 6M compared with baseline at diagnosis. Absolute lymphocytosis (ALC) was defined as lymphocyte > 4,000 /µL at 3 or 6M after the start of TKI. Pts were assessed for response, overall survival (OS), event-free survival (EFS), transformation-free survival (TFS), and failure-free survival (FFS) based on ALC and RLC. The Kaplan-Meier method was used to calculate OS, EFS, TFS, and FFS. A log-rank test and Cox regression were used for univariate (UVA) and multivariate analysis (MVA), respectively. Results: A total of 483 pts were enrolled in this study: 271 in IM, 105 in Nilo, and 107 in Dasa. Patient characteristics and outcomes are summarized in Table 1. Median age at diagnosis was 48 years, and median follow-up was 85M and ongoing (5-154+). Time from diagnosis to start of TKI, Sokal risk score, and ALC at baseline between groups did not differ clinically. Of 481 pts, 93 (19%) developed RLC at 3 or 6M; IM, 38 (14%); Nilo, 23 (22%); Dasa, 32 (30%) (p= .001). ALC at 3 or 6M was observed in 15 (3%); IM, 3 (1%); Nilo, 1 (1%); Dasa, 11 (10%) (p<.001). Overall, cumulative incidence of complete cytogenetic response (CCyR) at 6M, major molecular response (MMR) at 12M, molecular response with 4.5 log reduction by IS (MR4.5) at 24M did not differ significantly between RLC and non-RLC (3 or 6M), or between ALC and non-ALC (3 or 6M). 5-y TFS, EFS and OS in ALC group were significantly worse than those in non-ALC group (p= .002, p=.016, p=.008, respectively). By UVA and MVA related to OS, age [p <.001; Hazard ratio (HR), 1.062; 95% confidence interval (95%CI), 1.036-1.089], presence of ALC at 3 or 6M [p = .028; HR, 10.948; 95%CI, 1.297-92.415], absence of MMR at 24M [p=.016; HR, 2.263; 95%CI, 1.165-4.393] were identified as adverse prognostic factors for OS. Conclusion: The presence of ALC ≥4,000/µL at 3 or 6M of TKI therapies is rare but is adversely associated with overall survival. Table 1. Patient Characteristics and Outcomes (N=483)a Overall [n= 481] IM [n= 271] Nilo [n= 105] Dasa [n= 107] Age, (year) 48 (15-85) 48 (15-85) 49 (17-82) 48 (16-83) Sokal Risk, No. (%) Low 334 (69) 175 (65) 79 (75) 80 (75) Intermediate 114 (24) 74 (27) 18 (17) 22 (21) High 32 (7) 20 (7) 8 (8) 4 (4) Time from diagnosis to start of TKI, (M) 0.9 (0-12.6) 1.0 (0-12.6) 0.5 (0-5.6) 0.7 (0.1-7.8) ALC at baseline, (/109L) 2.5 (0-86.6) 2.4 (0-16.7) 2.6 (0.4-9.2) 2.7 (0.3-86.6) Incidence of Relative Lymphocytosis, No. (%) At 3M 65 (14) 25 (9) 16 (15) 24 (22) At 6M 76 (16) 32 (12) 20 (19) 24 (22) Overall 93 (19) 38 (14) 23 (22) 32 (30) Incidence of Absolute Lymphocytosis, No. (%) At 3M 8 (2) 1 (0) 0 7 (7) At 6M 11 (2) 3 (1) 1 (1) 7 (7) Overall 15 (3) 3 (1) 1 (1) 11 (10) Outcomes of RLC and ALC at any time in each group, +/- (%/%) (p) <10% BCR-ABL/ABL at 3M RLC 36/40 (.596) 22/44 (.213) 50/37 (.280) 31/38 (.537) ALC 38/39 (.952) 0/42 (.394) 100/39 (.214) 36/35 (.952) Cumulative CCyR at 6M RLC 75/75 (.288) 50/66 (.063) 96/90 (.413) 90/87 (.628) ALC 67/75 (.711) 33/64 (.276) 0/92 (.001) 82/89 (.599) Cumulative MMR at 12M RLC 67/74 (.406) 53/70 (.030) 83/82 (.921) 72/74 (.903) ALC 60/73 (.488) 33/68 (.197) 0/83 (.033) 73/74 (.745) Cumulative MR4.5 at 24M RLC 46/52 (.564) 37/50 (.139) 57/55 (.889) 50/57 (.729) ALC 33/52 (.332) 33/48 (.610) 0/56 (.264) 36/57 (.252) 5-y FFS RLC 61/71 (.133) 56/69 (.167) 62/70 (.710) 61/74 (.285) ALC 50/69 (.076) 0/68 (<.001) 0/70 (<.001) 71/70 (.974) 5-y TFS RLC 90/93 (.369) 88/93 (.597) 91/88 (.115) 91/99 (.213) ALC 72/93 (.002) 67/93 (.014) 0/90 (<.001) 80/97 (.121) 5-y EFS RLC 80/86 (.213) 71/83 (.154) 84/87 (.450) 86/93 (.486) ALC 64/85 (.016) 33/82 (<.001) 0/87 (<.001) 80/92 (.574) 5-y OS RLC 89/93 (.068) 81/94 (.007) 100/84 (.126) 96/99 (.207) ALC 82/93 (.008) 67/93 (.001) 100/88 (.847) 83/99 (.040) a Two in IM and 1 in Dasa were not evaluable due to lack of differential data at 3 and 6M. Figure 1. OS in Pts with ALC Figure 1. OS in Pts with ALC Disclosures O'Brien: Amgen, Celgene, GSK: Consultancy; CLL Global Research Foundation: Membership on an entity's Board of Directors or advisory committees; Emergent, Genentech, Gilead, Infinity, Pharmacyclics, Spectrum: Consultancy, Research Funding; MorphoSys, Acerta, TG Therapeutics: Research Funding.
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Bermejo, Begoña, Amparo Ruiz, Manuel Ruiz Borrego, Nuria Ribelles, Alvaro Rodriguez-Lescure, Montserrat Munoz-Mateu, Sonia Gonzalez, et al. "Randomized phase III study of adjuvant chemotherapy for node-positive early breast cancer (BC) patients (pts) comparing epirubicin plus cyclophosphamide followed by docetaxel (EC-T) versus epirubicin plus docetaxel followed by capecitabine (ET-X): Efficacy analysis of the GEICAM/2003-10 trial." Journal of Clinical Oncology 31, no. 15_suppl (May 20, 2013): 1027. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.1027.
Abstract:
1027 Background: X is an active drug in metastatic breast cancer. GEICAM/2003-10 is an adjuvant trial investigating the integration of capecitabine into an epirubicin and docetaxel containing regimen for node-positive early breast cancer pts. Methods: Pts aged 18-70, with T1-T3/N1-3 operable BC were eligible. HER2+ pts were initially allowed. In October 2005, after 803 pts were included in the trial, the study was amended to exclude them. Pts were stratified by site, menopausal status, number of axillary nodes (1-3, 4-9, >9) and hormonal receptor status and randomized to receive EC (90/600 mg/m2 x4) followed by T (100 mg/m2 x4) or ET (90/75 mg/m2 x4) followed by X (1,250 mg/m2 BID, d1–14, x 4) all every three weeks. The primary endpoint was DFS. The trial was designed to detect an absolute 5-y DFS increase of 7% (72% EC-T, 79% ET-X); a sample size of 1,184 evaluable pts (592 per arm) was required to detect this difference (a=0.05, β=80%). Assuming a drop-out rate of 17%, 1,382 pts were required. The first analysis of DFS was planned after 290 events. Results: Between February 2004 and February 2007, 1384 pts (EC-T 669, ET-X 715) were randomized. Patient characteristics were balanced between arms, median age was 51, 84% of pts were HR positive and 11% HER2 positive; 66, 25 and 9% had 1-3, 4-9 and > 9 nodes respectively. The median relative dose intensity was 99% for EC, 99% for T, 99% for ET and 94% for X. The most frequent grade 3-4 toxicities (>5% in either arm) with EC-T vs. ET-X were neutropenia (19% vs. 10%) with 7% febrile neutropenia in both arms, hand-foot syndrome (2% vs. 20%), fatigue (13% vs. 11%), diarrhea (3% vs. 11%), stomatitis (6% vs. 5%) and vomiting (5% vs. 5%). After a median follow-up of 6.6 years and 292 events, the proportion of patients disease free at 5 years is 86% and 82% with EC-T and ET-X (HR for relapse 1.314, 95% CI: 1.042 – 1.657); log-rank p-value=0.0208. Overall survival was not different between treatment arms (HR 1.113, 95% CI: 0.809 – 1.531); log rank p-value=0.511. Conclusions: DFS has been in favour of EC-T in pts with node-positive early BC. Clinical trial information: NCT00129935.
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Geerdink, Lianne M., Tammo Delhaas, Willem A. Helbing, Gideon J. du Marchie Sarvaas, Henriette ter Heide, Lieke Rozendaal, Chris L. de Korte, Petronella G. M. Peer, Irene M. Kuipers, and Livia Kapusta. "Paediatric Ebstein’s anomaly: how clinical presentation predicts mortality." Archives of Disease in Childhood 103, no. 9 (March 22, 2018): 859–63. http://dx.doi.org/10.1136/archdischild-2017-313482.
Abstract:
BackgroundForecasting the prognosis of a child when diagnosed with Ebstein’s anomaly is difficult. We, therefore, studied which factors at the time of diagnosis are associated with death during childhood.MethodsAll consecutive patients (0–18 years) diagnosed with Ebstein’s anomaly in the Netherlands between 1980 and 2014 were included. Survival curves were obtained using the Kaplan-Meier method. By using the Cox proportional hazard model, we analysed the factors (at diagnosis) that were associated with death.ResultsWe included 176 patients. Thirty-one patients (18%) died before the age of 18 years. The 1-year survival was 84% and remained stable at 82% from 35 months after diagnosis and onwards. Modified Ross Heart Failure Class 4 at the time of diagnosis was the most important risk factor for death during childhood (HR 12.5, 95% CI 4.4 to 35.9). Furthermore, diagnosis in the neonatal period (HR 4.2, 95% CI 1.5 to 12.0), severe tricuspid valve regurgitation (HR 2.4, 95% CI 1.2 to 5.0), severe right ventricular outflow tract obstruction (HR 3.7, 95% CI 1.8 to 7.7) and a patent ductus arteriosus (HR 2.8, 95% CI 1.3 to 6.0) at the time of diagnosis were univariately associated with death. Multivariable analysis showed that presentation with Heart Failure Class 4 and a ventricular septal defect is the strongest predictor of death in childhood and adolescence.ConclusionPatients with Ebstein’s anomaly presenting with Heart Failure Class 4 and a ventricular septal defect have a high risk of death during childhood.
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Silvis, Suzanne M., Erik Lindgren, Sini Hiltunen, Sharon Devasagayam, Luuk J. Scheres, Katarina Jood, Susanna M. Zuurbier, et al. "Postpartum Period Is a Risk Factor for Cerebral Venous Thrombosis." Stroke 50, no. 2 (February 2019): 501–3. http://dx.doi.org/10.1161/strokeaha.118.023017.
Abstract:
Background and Purpose— Pregnancy and the postpartum period are generally considered to be risk factors for cerebral venous thrombosis (CVT), but no controlled studies have quantified the risk. Methods— Case-control study using data of consecutive adult patients with CVT from 5 academic hospitals and controls from the Dutch MEGA study (Multiple Environmental and Genetic Assessment of risk factors for venous thrombosis). Men, women over the age of 50, women using oral contraceptives or with a recent abortion or miscarriage were excluded. We adjusted for age and history of cancer, and stratified for pregnancy versus postpartum, and 0 to 6 versus 7 to 12 weeks postpartum. Results— In total 163/813 cases and 1230/6296 controls were included. Cases were younger (median 38 versus 41 years) and more often had a history of cancer (14% versus 4%) than controls. In total 41/163 (25%) cases and 82/1230 (7%) controls were pregnant or postpartum (adjusted odds ratio, 3.8; 95% CI, 2.4–6.0). The association was fully attributable to an increased risk of CVT during the postpartum period (adjusted odds ratio, 10.6; 95% CI, 5.6–20.0). We found no association between pregnancy and CVT (adjusted odds ratio, 1.2; 95% CI, 0.6–2.3). The risk was highest during the first 6 weeks postpartum (adjusted odds ratio, 18.7; 95% CI, 8.3–41.9). Conclusions— Women who have recently delivered are at increased risk of developing CVT, while there does not seem to be an increased risk of CVT during pregnancy.
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Traykov, Vassil, Svetoslav Iovev, Borislav Borisov, Ivaylo Kozhuharov, Momchil Marinov, and Vasil Velchev. "Cardiac implantable electronic devices in Bulgaria: results from the electronic registry BG-Pace for the period 2019-2021." Bulgarian Cardiology 27, no. 3 (October 18, 2021): 69–87. http://dx.doi.org/10.3897/bgcardio.27.e71177.
Abstract:
The current study analyses the activity in cardiac pacing in Bulgaria in 2019, 2020 and 2021 based on data derived from a national registry of patients with cardiac electronic implantable devices (CIEDs). Materials and methods. Data from the national registry BG-Pace in the period 08.2019-06.2021 was retrospectively studied. Demographic data, procedure and device type, pacing mode, etiology, symptoms, preimplantation ECG, number of implantations and centre and operator volumes were analysed. Results. Six thousand nine hundred forty-nine devices were implanted by 47 operators in 28 centres for the study period. Median age was 75 (IQR 68-81, 21-103) years in males and 77 (IQR 71-82, 17-98) years in females, P ‹ 0.05. The largest number of devices were implanted in the age group 70-79 years. The most commonly implanted CIEDs were antibradycardia devices with a total of 486.7/million implantations for the period 08.2019-08.2020 and 353.9/million for the period 08.2020-06.2021. Implanted cardioverter-defibrillators demonstrated a growth from 14.1/million to 20.1/million in the period 08.2020 – 06.2021. The number of all implantations dropped signifi cantly during the two epidemic waves of COVID-19. The mean number of implantations per centre and per operator for the whole period was 232.3±204 (2-705) and 148±139.1 (2-660), respectively. Dual chamber device implantations were more prevalent, representing 65.8% of implantations in AV block I and II degree, 63.5% in complete AV block, 59.8% in intraventricular conduction disturbances and 60.9% in sick sinus syndrome. Pacing modes with atrial sensing represented more than 55% of the implantations for all indications. Increasing age was associated with signifi cantly more common use of VVI pacing mode (P ‹ 0.001). Conclusion. The national registry BG-Pace includes systematic clinical, demographical and procedural data for CIED implantations in Bulgaria. Results demonstrate lower number of implantations compared to the average European volume. There was a signifi cant drop in the implantation rate during the two waves of COVID-19.
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Fiala, Mark A., Tanya M. Wildes, Michael Slade, Jesse Keller, Keith Stockerl-Goldstein, Michael Tomasson, and Ravi Vij. "Variations in Multiple Myeloma Disease Presentation By Race." Blood 126, no. 23 (December 3, 2015): 5618. http://dx.doi.org/10.1182/blood.v126.23.5618.5618.
Abstract:
Abstract Background: Black patients with multiple myeloma (MM) have poorer outcomes than their white counterparts. This has largely been attributed to reduced access to health care; however, little data exists comparing the disease and overall health status at MM presentation between the two races. More severe disease burden, symptom burden, or comorbidities could also explain the differences in outcome. Objective: To compare disease burden, symptom burden, and comorbidities between black and white patients with MM. Methods: Two datasets were analyzed: 1) the Multiple Myeloma Research Foundation (MMRF) CoMMpass study interim analysis 6, and 2) the Surveillance, Epidemiology, and End Results-Medicare Health Outcomes Survey (SEER-MHOS) 2015 dataset (SEER years 1973-2011; MHOS years 1998-2013). The CoMMpass dataset included 625 patients who completed the EORTC QLQ-C30 and QLQ-MY20 at MM diagnosis. The SEER-MHOS dataset included 377 patients who completed the HOS survey the year of or year prior to MM diagnosis. All patients identified as a race other than white or black/African American were excluded. Data was analyzed using SPSS 21. Categorical variables were compared using χ2, continuous with the Mann-Whitney U test. Results: CoMMpass: 585 patients were eligible for analysis. 477 (82%) were white, 108 (19%) were black. Whites and blacks were similar in median age, but a significantly higher percentage of white patients were female (p=0.027). Overall, black patients were more likely to be stage III (p=0.041), have higher LDH (p=0.006) and creatinine (p=0.001), and lower hemoglobin (p<0.001), but were more likely to have CD117+ MM cells (p=0.049). While quality of life measures were similar between the two races, white race was associated with better performance status (p=0.021). Results are summarized in Table 1. SEER-MHOS: 275 patients were eligible for analysis. 234 (85%) were white, 41 (15%) were black. Whites and blacks were similar in median age and sex distribution; there were no significant (p<0.05) differences in quality of life measures or comorbidities between the two races. Results are summarized in Table 2. Conclusions: The presentation of MM was similar between blacks and whites, but black patients tended to have a higher disease burden than whites. Despite this, both races reported similar symptom burden. Further studies are required to determine if higher disease burden account for part of the outcome disparities seen between the two races. Table 1. CoMMpass Whiten= 477 Black n = 108 p Demographics Age in years 65 63 NS Female 63% 49% 0.027 Disease Burden ISS Stage 0.041 Stage I/II 71% 61% Stage III 29% 40% Heavy Chain NS IgG 78% 81% IgA 22% 19% Light Chain NS Kappa 60% 73% Lambda 38% 27% Biclonal 2% 0% Serum M-Protein g/dL 2.9 2.5 NS LDH ukat/L 2.8 3.0 0.006 Bone Marrow Plasma Cells* 9% 9% NS Circulating Plasma Cells* 0% 0% NS Calcium mmol/L 2.4 2.4 NS Creatinine umol/L 93 111 0.001 Hgb mmol/L 6.7 6.0 <0.001 Platelets x109/L 213 207 NS Bone Lesions 56% 51% NS Molecular Characteristics Abnormal Karyotype 43% 39% NS Deletion 13 31% 32% NS Deletion 17p 20% 16% NS Translocation 11;14 19% 14% NS Translocation 4;14 10% 8% NS Phenotype** CD13+ 22% 27% NS CD20+ 17% 10% NS CD33+ 27% 29% NS CD52+ 12% 10% NS CD56+ 78% 79% NS CD117+ 56% 67% 0.049 FGFR3+ 15% 13% NS Symptom Burden/Quality of Life ECOG Performance Status 0.021 0-1 87% 78% 2-4 13% 22% Global Health Scale 58 66 NS Physical Functioning Scale 80 73 NS Cognitive Functioning Scale 83 83 NS Emotional Functioning Scale 75 83 NS Social Functioning Scale 66 66 NS Role Functioning Scale 66 50 NS Disease Symptom Scale 22 27 NS Fatigue Scale 33 33 NS Pain Scale 33 33 NS Median presented unless specified *- CD38+/CD138+ by flow cytometry **- performed on CD38+/CD138+ bone marrow cells Table 2. SEER-MHOS Whiten= 234 Black n = 41 p Demographics Age in years 77 75 NS Female 46% 41% NS Symptom Burden/Quality of Life General Health Scale 54 59 NS Physical Functioning Scale 58 70 NS Emotional Well-Being Scale 80 82 NS Social Functioning Scale 75 88 NS Role Limitation Physical Scale 25 50 NS Role Limitation Emotional Scale 100 100 NS Energy/Fatigue Scale 50 55 NS Pain Scale 51 57 NS Comorbidities Prior Malignancy 21% 10% NS Hypertension 60% 68% NS Coronary Artery Disease 16% 8% NS Congestive Heart Failure 7% 0% NS Myocardial Infarction 12% 10% NS Cerebral Vascular Accident 10% 10% NS Chronic Obstructive Pulmonary Disease 13% 12% NS Diabetes 81% 78% NS Obesity 20% 11% NS Median presented unless specified Disclosures Vij: Takeda, Onyx: Research Funding; Celgene, Onyx, Takeda, Novartis, BMS, Sanofi, Janssen, Merck: Consultancy.
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Boccardo, F., A. Rubagotti, P. Guglielmini, P. Sismondi, A. Farris, D. Amadori, B. Agostara, A. Gambi, G. Catalano, and M. Faedi. "Epirubicin (E) followed by cyclophosphamide, methotrexate, 5-fluorouracil (CMF) versus paclitaxel (T) followed by epirubicin and vinorelbine (EV) in patients (pts) with high-risk operable breast cancer." Journal of Clinical Oncology 27, no. 15_suppl (May 20, 2009): e11521-e11521. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.e11521.
Abstract:
e11521 Background: > 3 N+ pts have a poor outcome, even in the presence of otherwise favourable phenotypic features. CT, alone or combined with endocrine therapy (ET) in hormone receptor (HOR)+ pts, is the gold standard for these women. However questions still exist about the optimal CT regimen. Methods: 244 >3N+ pts were randomized to either four 3-weekly courses of E (100 mg/sqm,d 1)followed by four 4-weekly cycles of CMF (600, 40, 600 mg/sqm, dd 1,8)(E-CMF:N=122)or four 3-weekly courses of T (175 mg/sqm, d1) followed by four 3-weekly cycles of EV (E:75 mg/sqm, da 1; V: 25 mg/sqm dd 1,8;.) (T-EV:N=122). After CT, tamoxifen (plus a LH-RH analog in menstruating women ) was given for 5 years to all HOR+ pts.S was the primary end-point. Results: At 82 mos median f-up, S and RFS did not differ significantly between groups (7-yr S: E-CMF:76%,T- EV:74%;adjust. HR: 0.89;0.54–1.49;p=0.7; 7-yr RFS: E-CMF:67%,T-EV:63%;adjust. HR: 0.95;0.61–1.47;p=0.8;).Cox analysis confirmed no difference between treatments. However RFS forest plots showed some heterogenity in CT effect according to HOR and HER2 status: E- CMF was superior to E-TV in HOR + pts (HR=0.43; CI 0.20–0.93 p=0.03),irrespective of HER2 status, but it was inferior (HR=1.91;CI 0.48- 7.64;p=0.4) in triple negative pts. The two regimens showed different toxicity profiles;however toxicities were manageable and there were no difference in the %of pts receiving all the 8 planned CT cycles (E-CMF: 87.6%;T-EV:81.1% p=0.2) or who discontinued CT (E-CMF: 12.8%; T-EV: 18.9% p=0.2). Conclusions: T-EV was not superior to E-CMF which,combined with ET, still represents an adequate choice for this high risk pts subset, especially for HOR+ pts. No significant financial relationships to disclose.
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Kämpfe, Dietrich, Harald Stein, Roshanak Bob, Sebastian Böttcher, Joerg Brenn, Agnes Knopp, Thomas Haverkamp, Christoph Schulte, and Detlef Haase. "Real Life Dates of Chronic Lymphocytic Leukemia (CLL) in Germany. Interim Analysis of 234 Consecutively New Diagnosed Patients in a German Epidemiological-Clinical Register." Blood 132, Supplement 1 (November 29, 2018): 2264. http://dx.doi.org/10.1182/blood-2018-99-110618.
Abstract:
Abstract Background: Therapy studies yield important insights into clinical features and therapeutic options of CLL. However studies in Germany represent less then 20% of all CLL patients (pts); pts >75 years (yrs) are included in 3% only. Furthermore initial phases are not reflected in multicenter studies and data representing real life situation are scarce. Methods: Out of 1.443 patients consulting our center because of leucocytosis (>10 Gpt/l) between 2003/07/01 and 2018/06/30 we diagnosed CLL consecutively in 234 pts (16,2%): 143 male (m), 91 female (f). Median age at diagnosis is 71,4 yrs. Median leucocyte count is 18,0 Gpt/l. BINET - Stages are: A in 82%, B in 11% and C in 7% of pts. Diagnoses are strictly based on WHO definition: Pts with small lymphocytic lymphomas (sLL, n=11) are included but those with monoclonal lymphocytosis of unknown significance (MLUS) or monoclonal B - lymphocytoses with uncertainly flow cytometry (FC) results are excluded. Histopathology, FC and genetics have been performed by external hematological reference laboratories. All investigations followed the rules of best clinical and laboratory practice. Dates of death are given by the record sections of the involved communities (deadline 2018/06/30). To address different questions we defined 3 groups of pts: A: "collective group", (n=234), i.e. all pts.diagnosed from 2003/07/01 to 2018/06/30. B: "epidemiological group" (n=129), i.e. pts from 3 communities (113.000 inhabitants - inc - in 2009/12/31) in close proximity referred to our center. C: "genetic features group" (n=99), i.e. pts diagnosed continuously between 2012/07/01 and 2018/06/30 with systematically performed genetics, (i.e. at least 80% of pts in this group. Genetics are: Cytogenetic (banding) n=88; FISH (del 6q21/6q, del 11q22.3, +12/+12q, del 13q14/ 13q34, del 17p13.1, 14q32) n=85. PCR (IgVH status - mutated vs. unmutated; TP53, NOTCH1, SF3B1 mutation) n=82. Results: "Collective group" (A): 7,5 years OS is 72% and 15 years OS is 33% respectively (KAPLAN-MEIER). Age of pts acts as a predominant factor for long term OS: 77% in pts < 60 yrs vs. 40% in pts ≥60 to <75 yrs vs. 16% in pts ≥75 yrs. 15 yrs OS of treated pts is 32% (n=78, median age at diagnosis: 73,3 yrs, m : f = 1,50); 15 yrs OS of untreated patients is 33% (n=146, median age at diagnosis: 71,0 yrs, m : f = 1,56). Mean time to 1st treatment was 16,0 month (0,1 to 132,6 months). "Epidemiological group" (B): Raw incidence is 7,56/105 inh. (m: 9,13, f: 6,06), age adjusted incidence is 10,3/105 inh (m: 12,5, f: 8,3), with highest rate of age between 75 to < 80 yrs. Standard incidence is calculated as 6,27/105 (BRD 1987), 4,78/105 (Europe) and 5,06/105 (USA 2000). Prevalence could be measured directly (2017-12-31): 79,1/105 inh. "Genetic features group" (C): IgVH mutation status is hypermutated in 48 pts. vs. unmutated in 39 pts. Results of FISH analyses (n = 85) are: del 6: 5,5%, del 11: 17,6%, +12: 3%, del 13q: 70,3%, del 17p: 8,8%; normal status: 13,2%. PCR revealed mutations of NOTCH1 in 7%, of SF3B1 in 10,5% and of TP53 in 8,6% of 82 pts, respectively. Combined del17p by FISH and molecular TP53 mutations at diagnosis are found in 3 of 81 pts (3,7%). Cytogenetic findings different to results of FISH and/or PCR were found in 36 of 82 pts (43,9%). Calculated 5 yrs freedom of therapy is 75% in IgVH hypermutated pts vs. 45% in unmutated pts (p = 0,0005; log rank test); calculated 5 yrs OS is 92% vs. 59% (p=0,023). Special situations in CLL: 13 of 234 pts (5,5%) have one parent or sibling with low grade NHL, mostly CLL. In contrast there is non spouse suffering of CLL / low grade NHL. Deficiency in Immunoglobuline G (Ig G < 4,0 g/l) was found in 17 of 199 pts at diagnose (8,5%), 29 pts (12,4%) were substituted with Ig G's in the Course of CLL. In 14 (6,0%) pts. we diagnosed a 2nd hematologic malignancy (3x cMPN, 1x CML, 3x MDS, 3x AML, 1x FL, 1x MCL, 1x MZL, 1x DLCBL-independent of CLL; monoclonal gammopathies are not considered). RICHTER syndrome was found in 1 case, but progression to plasmocytic/plasmoblastic disease was seen in 3 cases. Li FRAUMENI Syndrome was revealed in 1 male patient, he has been in stable disease for 13 yrs. Conclusion: CLL seems to be more frequent than yet considered. IgVH mutation status seems to be very important as a single prognostic factor concerning time of 1st therapy as well as OS. Clinical features of CLL are very different and impressing. Disclosures Böttcher: Genentech: Research Funding; Janssen: Honoraria; AbbVie: Honoraria, Research Funding; Celgene: Research Funding; Roche: Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding.
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Edgcomb, Juliet Beni, Chi-hong Tseng, Mengtong Pan, Alexandra Klomhaus, and Bonnie T. Zima. "Assessing Detection of Children With Suicide-Related Emergencies: Evaluation and Development of Computable Phenotyping Approaches." JMIR Mental Health 10 (July 21, 2023): e47084. http://dx.doi.org/10.2196/47084.
Abstract:
Background Although suicide is a leading cause of death among children, the optimal approach for using health care data sets to detect suicide-related emergencies among children is not known. Objective This study aimed to assess the performance of suicide-related International Classification of Diseases, Tenth Revision, Clinical Modification (ICD-10-CM) codes and suicide-related chief complaint in detecting self-injurious thoughts and behaviors (SITB) among children compared with clinician chart review. The study also aimed to examine variations in performance by child sociodemographics and type of self-injury, as well as develop machine learning models trained on codified health record data (features) and clinician chart review (gold standard) and test model detection performance. Methods A gold standard classification of suicide-related emergencies was determined through clinician manual review of clinical notes from 600 emergency department visits between 2015 and 2019 by children aged 10 to 17 years. Visits classified with nonfatal suicide attempt or intentional self-harm using the Centers for Disease Control and Prevention surveillance case definition list of ICD-10-CM codes and suicide-related chief complaint were compared with the gold standard classification. Machine learning classifiers (least absolute shrinkage and selection operator–penalized logistic regression and random forest) were then trained and tested using codified health record data (eg, child sociodemographics, medications, disposition, and laboratory testing) and the gold standard classification. The accuracy, sensitivity, and specificity of each detection approach and relative importance of features were examined. Results SITB accounted for 47.3% (284/600) of the visits. Suicide-related diagnostic codes missed nearly one-third (82/284, 28.9%) and suicide-related chief complaints missed more than half (153/284, 53.9%) of the children presenting to emergency departments with SITB. Sensitivity was significantly lower for male children than for female children (0.69, 95% CI 0.61-0.77 vs 0.84, 95% CI 0.78-0.90, respectively) and for preteens compared with adolescents (0.66, 95% CI 0.54-0.78 vs 0.86, 95% CI 0.80-0.92, respectively). Specificity was significantly lower for detecting preparatory acts (0.68, 95% CI 0.64-0.72) and attempts (0.67, 95% CI 0.63-0.71) than for detecting ideation (0.79, 95% CI 0.75-0.82). Machine learning–based models significantly improved the sensitivity of detection compared with suicide-related codes and chief complaint alone. Models considering all 84 features performed similarly to models considering only mental health–related ICD-10-CM codes and chief complaints (34 features) and models considering non–ICD-10-CM code indicators and mental health–related chief complaints (53 features). Conclusions The capacity to detect children with SITB may be strengthened by applying a machine learning–based approach to codified health record data. To improve integration between clinical research informatics and child mental health care, future research is needed to evaluate the potential benefits of implementing detection approaches at the point of care and identifying precise targets for suicide prevention interventions in children.
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Magnavita, Nicola, Giovanni Tripepi, and Reparata Rosa Di Prinzio. "Symptoms in Health Care Workers during the COVID-19 Epidemic. A Cross-Sectional Survey." International Journal of Environmental Research and Public Health 17, no. 14 (July 20, 2020): 5218. http://dx.doi.org/10.3390/ijerph17145218.
Abstract:
In March–April 2020, the Corona Virus Disease 19 (COVID-19) pandemic suddenly hit Italian healthcare facilities and in some of them many staff members became infected. In this work 595 health care workers from a public company were tested for Severe acute respiratory syndrome coronavirus 2 (82 positive) and asked to complete a questionnaire on early COVID-19 symptoms. Respiratory symptoms were present in 56.1% of cases. Anosmia and dysgeusia in COVID-19 cases were found to have an odds ratio (OR) = 100.7 (95% Confidence Interval [CI] = 26.5–382.6) and an OR = 51.8 (95%CI 16.6–161.9), respectively. About one in three of the cases (29.3%) never manifested symptoms. Anxiety was reported by 16.6% of COVID-19 cases and depression by 20.3%, with a significant increase in the estimated risk (OR = 4.3; 95%CI = 2.4–7.4 for anxiety, OR = 3.5; 95%CI = 2.0–6.0 for depression). In cases, sleep was a significant moderating factor in the relationship between occupational stress, or organizational justice, and anxiety. The early diagnosis of COVID-19 in health care workers, must consider, in addition to respiratory disorders and fever, anosmia, dysgeusia, exhaustion, myalgias and enteric disorders. The frequency of anxiety and depression disorders in the population examined was not higher than that commonly recorded in the same company during periodic checks in the years preceding the epidemic. In COVID-19 cases there was a significant risk of anxiety, especially in those who had low sleep quality. Mental health support and improvement interventions must mainly concern workers with positive tests and should also tend to improve sleep quality.
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Yu, Christopher, Austin C. C. Ng, Lloyd Ridley, Mekhala Anjaria, Silvan Meier, John Yiannikas, Leonard Kritharides, and Christopher Naoum. "Incidentally identified coronary artery calcium on non-contrast CT scan of the chest predicts major adverse cardiac events among hospital inpatients." Open Heart 8, no. 2 (October 2021): e001695. http://dx.doi.org/10.1136/openhrt-2021-001695.
Abstract:
BackgroundCoronary artery calcium (CAC) identified on non-gated CT scan of the chest is predictive of major adverse cardiac events (MACE) in multiple studies with guidelines therefore recommending the routine reporting of incidental CAC. These studies have been limited however to the outpatient setting. We aimed to determine the prognostic utility of incidentally identified CAC on CT scan of the chest among hospital inpatients.Methods and resultsConsecutive patients (n=740) referred for inpatient non-contrast CT scan of the chest at a tertiary referral hospital (January 2011 to March 2017) were included (n=280) if they had no known history of coronary artery disease, active malignancy or died within 30 days of admission. Scans were assessed for the presence of CAC by visual assessment and quantified by Agatston scoring. Median age was 69 years (IQR: 54–82) and 51% were male with a median CAC score of 7 (IQR 0–205). MACE occurred in 140 (50%) patients at 3.5 years median follow-up including 98 deaths. Half of all events occurred within 18 months. Visible CAC was associated with increased MACE (HR) 6.0 (95% CI: 3.7 to 9.7) compared with patients with no visible CAC. This finding persisted after adjusting for cardiovascular risk factors HR 2.4 (95% CI: 1.3 to 4.3) and with both absolute CAC score and CAC score ≥50th percentile.ConclusionIncidental CAC identified on CT scan of the chest among hospital inpatients provides prognostic information that is independent of cardiovascular risk factors. These patients may benefit from aggressive risk factor modification given the high event rate in the short term.
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Withrow, Kirk, Sean Evans, John Harwick, Eric Kezirian, and Patrick Strollo. "Upper Airway Stimulation Response in Older Adults with Moderate to Severe Obstructive Sleep Apnea." Otolaryngology–Head and Neck Surgery 161, no. 4 (May 14, 2019): 714–19. http://dx.doi.org/10.1177/0194599819848709.
Abstract:
Objective To evaluate the impact of age on safety, efficacy, and usage of upper airway stimulation (UAS). Study Design Multicenter observational study. Setting Thirteen US hospitals and 3 German hospitals. Subjects and Methods The ADHERE registry is a multicenter database enrolling patients undergoing UAS implantation from October 2016 to April 2018. Outcome measures included the Epworth Sleepiness Scale, apnea-hypopnea index (AHI), therapy usage, and complications. Data were segmented by age (<65 vs ≥65 years). Results Younger adults (n = 365) were a mean ± SD 52.7 ± 7.9 years old and 82% male, with a body mass index of 29.6 ± 3.8. Older adults (n = 235) were 71.1 ± 4.8 years old and 71% male, with a body mass index of 28.8 ± 3.8. Baseline AHI was similar (younger, 36.2 ± 15.9; older, 36.1 ± 14.8). Both groups had lower AHI at 12 months versus baseline ( P < .001), but the older group showed a greater reduction (7.6 ± 6.9 vs 11.9 ± 13.4, P = .01). The Epworth Sleepiness Scale score decreased from 12.3 ± 5.4 to 7.1 ± 4.8 ( P < .001) among younger adults and from 10.7 ± 5.7 to 6.3 ± 4.4 ( P < .001) among older adults. Usage was slightly higher among older adults (6.0 ± 2.0 vs 5.4 ± 2.1 hours/night, P = .02). Surgical time was similar between younger patients (2.4 ± 0.7 hours) and older patients (2.3 ± 0.7 hours, P = .40), with comparably low complications. Conclusion AHI reduction and therapy usage were found to be somewhat higher among patients aged ≥65 years who were treated with UAS. Surgical complications were low, in contrast to traditional sleep surgery.
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de Castro, Viviane, Daniel Fuentes, and Hermano Tavares. "The Gambling Follow-up Scale: Development and Reliability Testing of a Scale for Pathological Gamblers under Treatment." Canadian Journal of Psychiatry 50, no. 2 (February 2005): 81–86. http://dx.doi.org/10.1177/070674370505000202.
Abstract:
Objective: To provide preliminary data on the Gambling Follow-Up Scale (GFS), a new scale assessing recovering gamblers. Secondary goals included assessing the impact of “work status,” “family relationship,” “leisure,” and “enrolment in Gamblers Anonymous (GA)” on gambling (all items from the scale), together with the impact of treatment. Method: Using the GFS, 3 independent raters interviewed gamblers under treatment. The sample was collected in 2 university centres in the city of São Paulo, Brazil. Patients attended psychotherapy coupled with psychiatric follow-up, participation in GA, or both. Results: We interviewed 47 pathological gamblers; 13 were interviewed twice, with a minimum interval of 6 months, for a total of 60 GFS interviews. Interviews took on average 6.0 minutes, SD 2.7. Interrater concordance ranged from 82% to 95% (intraclass correlation coefficient range 0.85 to 0.99, P < 0.001). A factorial analysis showed a 1-factor solution (Eigenvalue = 2.4, 47.6% of total variance accounted). “Leisure,” “frequency and time gambling,” and “family relationship” showed considerable loadings (0.84; 0.71; 0.71), whereas “enrolment in GA” and “work status” showed moderate loadings (0.59; 0.56). A linear regression model significantly correlated gambling ( R2 = 0.356; P < 0.001) with “leisure” and length of treatment. Treatment modalities affected “leisure” ( F2,43 = 5.00, P = 0.011), with GA attendees reporting more regular and gratifying activities. Conclusions: The GFS showed interrater reliability and construct validity. More leisure and lengthier treatment were significantly related to less gambling. GA enrolment seemed to particularly benefit the quality of leisure. Future studies could profit from the quickness and simple structure of the GFS in providing shareable outcome measures.
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Gupta, Kashvi, Prasanna Mithra, B. Unnikrishnan, T. Rekha, Nithin Kumar, Abhinav Chaturvedi, Siddhant Shetty, Ravi Shankar, Richa Singh, and Ayesha. "IJCM_11A: Clinico-epidemiological profile of malignancies in the tertiary care centres in Mangalore." Indian Journal of Community Medicine 49, Suppl 1 (April 2024): S3—S4. http://dx.doi.org/10.4103/ijcm.ijcm_abstract11.
Abstract:
Background: The Hospital-based Cancer Registry (HBCR) part of the National Cancer Registry Programme provides the number of cancer cases attending the hospital in various disciplines and the exact type of cases classified, diagnosed and treated. This study was done to assess the socio-demographic, clinical profile, and trend of malignancy cases in the tertiary care centres of Mangalore. Methodology: This Record-based retrospective study was conducted in tertiary care hospitals affiliated with Kasturba Medical College, Mangalore and all the cases of malignancies admitted during the study period were included. The data were collected using a proforma and analyzed using SPSS version 25.0. The results were expressed as proportions using appropriate tables and graphs. Results: A total of 600 cancer cases were identified, with females comprising nearly half 48.8%(n=293) and among them the highest number of cases, 28% (n=82) were found in the age group 60 and above. Overall, cancer cases appear to be more prevalent in older age groups for both genders. Among males, the most common cancers were Oral cavity cancers 34.2%(n=105), followed by Gastrointestinal (GI) tract cancers,16.3%(n=50). In females the most common cancers were found to be of the breast followed by uterine cancer then by oral cavity Conclusion: The prevalence of cancer in both genders were found to be almost the same. The highest number of cases were reported from Karnataka followed by Kerala. The highest prevalence was found in higher extremes of age above 60 years.
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Williams Silva, Karly, Hanjie Shen, Eileen Rillamas-Sun, Cindy Lin, Lexi M. Harlow, Jonathan Siman, Susan Veleber, Sofia Cobos, Heather Greenlee, and Stacey Cohen. "Adopting the "Exercise as a Vital Sign" metric to assess physical activity in GI oncology patients." Journal of Clinical Oncology 40, no. 4_suppl (February 1, 2022): 650. http://dx.doi.org/10.1200/jco.2022.40.4_suppl.650.
Abstract:
650 Background: Regular moderate to vigorous physical activity (MVPA) in patients with gastrointestinal (GI) cancers is associated with improved health outcomes, including greater quality of life and reduced fatigue and comorbidity. PA guidelines for cancer survivors recommend ≥150 minutes of MVPA per week for optimal health. Identifying GI oncology patients with low MVPA is important to tailor interventions aimed at increasing MVPA levels in this population. The “Exercise as a Vital Sign” (EVS) tool is a validated, two question assessment tool to quantify minutes per week (min/wk) of MVPA that was incorporated in the clinical rooming process at an academic GI oncology clinic. Here, we report characteristics of patients by amount of MVPA by EVS. Methods: Patient information was entered in the electronic medical record at a large, academic oncology clinic. Data from the first clinic visit where min/wk of MVPA EVS was collected were extracted and included demographics (e.g. age, sex, marital status, race), health status (body mass index (BMI), Eastern Cooperative Oncology Group (ECOG) performance status, comorbidities), cancer characteristics (primary tumor site, localized/metastatic, current treatment), and reason for the clinic visit. MVPA was divided into two groups based on the recommended guidelines: 0-149 min/wk (“low”) and ≥150 min/wk (“high”). Chi-square and t-tests were used to examine statistically significant differences. Results: Among 265 patients abstracted, EVS data were reported from 156 (59%) patients. Mean (standard deviation (SD)) age was 61 (13) years (range: 25-82). The primary cancer site was 35% colorectal, 23% pancreatic, 17% gastroesophageal, 10% neuroendocrine, and 15% other GI cancers. While gastroesophageal cancer patients reported high amounts of MVPA (mean (SD) = 268 (309) min/wk), comparisons with other cancer sites were not statistically different. Of the 156 with EVS data, 84 (54%) had low MVPA (mean (SD): 55 (49) min/wk) and 72 (46%) had high MVPA (mean (SD): 357 (244) min/wk). Demographic and cancer characteristics were similar across the two groups. Patients with high MVPA were more likely to have BMI 18.5-24.9 kg/m2 (56% vs. 31%) and less likely to have BMI ≥25 kg/m2 (40% vs.61%) compared to patients with low MVPA (p = 0.02). Patients with high vs. low MVPA were also more likely to have excellent performance status (ECOG 0; 47% vs. 24%, p < 0.001). Conclusions: In this study, GI oncology patients not meeting PA guidelines for cancer survivors were more likely to be overweight/obese and have worse performance status. The EVS tool was an inexpensive and accessible measure of MVPA that can be used to identify patients with low MVPA for interventions targeted to improve health outcomes. A lack of statistical significance may be due to low power. At the conference, more data will be presented (̃1000 patient visits), including longitudinal data and MVPA by active chemotherapy regimen.
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Hoorntje, Alexander, P. Paul F. M. Kuijer, Berbke T. van Ginneken, Koen L. M. Koenraadt, Rutger C. I. van Geenen, Gino M. M. J. Kerkhoffs, and Ronald J. van Heerwaarden. "Prognostic Factors for Return to Sport After High Tibial Osteotomy: A Directed Acyclic Graph Approach." American Journal of Sports Medicine 47, no. 8 (June 3, 2019): 1854–62. http://dx.doi.org/10.1177/0363546519849476.
Abstract:
Background: High tibial osteotomy (HTO) is increasingly used in young and physically active patients with knee osteoarthritis. These patients have high expectations, including return to sport (RTS). By retaining native knee structures, a return to highly knee-demanding activities seems possible. However, evidence on patient-related outcomes, including RTS, is sparse. Also, time to RTS has never been described. Furthermore, prognostic factors for RTS after HTO have never been investigated. These data may further justify HTO as a surgical alternative to knee arthroplasty. Purpose: To investigate the extent and timing of RTS after HTO in the largest cohort investigated for RTS to date and to identify prognostic factors for successful RTS. Study Design: Case-control study; Level of evidence, 3. Methods: Consecutive patients with HTO, operated on between 2012 and 2015, received a questionnaire. First, pre- and postoperative sports participation questions were asked. Also, time to RTS, sports level and frequency, impact level, the presymptomatic and postoperative Tegner activity score (1-10; higher is more active), and the postoperative Lysholm score (0-100; higher is better) were collected. Finally, prognostic factors for RTS were analyzed using a logistic regression model. Covariates were selected based on univariate analysis and a directed acyclic graph. Results: We included 340 eligible patients of whom 294 sufficiently completed the questionnaire. The mean follow-up was 3.7 years (± 1.0 years). Out of 256 patients participating in sports preoperatively, 210 patients (82%) returned to sport postoperatively, of whom 158 (75%) returned within 6 months. We observed a shift to participation in lower-impact activities, although 44% of reported sports activities at final follow-up were intermediate- or high-impact sports. The median Tegner score decreased from 5.0 (interquartile range [IQR], 4.0-6.0) presymptomatically to 4.0 (IQR, 3.0-4.0) at follow-up ( P < .001). The mean Lysholm score at follow-up was 68 (SD, ± 22). No significant differences were found between patients with varus or valgus osteoarthritis. The strongest prognostic factor for RTS was continued sports participation in the year before surgery (odds ratio, 2.81; 95% CI, 1.37-5.76). Conclusion: More than 8 of 10 patients returned to sport after HTO. Continued preoperative sports participation was associated with a successful RTS. Future studies need to identify additional prognostic factors.
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Carvalho, Almy Junior Cordeiro de, Pedro Henrique Monnerat, Donivaldo Pedro Martins, Salassier Bernardo, and José Accácio da Silva. "Teores foliares de nutrientes no maracujazeiro amarelo em função de adubação nitrogenada, irrigação e epócas de amostragem." Scientia Agricola 59, no. 1 (March 2002): 121–27. http://dx.doi.org/10.1590/s0103-90162002000100018.
Abstract:
A realização de diagnósticos de culturas, através do conhecimento dos teores foliares dos nutrientes, em cada condição de cultivo, se justifica e tem por finalidade corrigir deficiências, inclusive com a utilização de ferramentas tais como o DRIS (Sistema Integrado de Diagnose e Recomendação), que auxiliam no manejo para a obtenção de altos rendimentos das culturas. Este trabalho teve por objetivo avaliar os efeitos da adubação nitrogenada, irrigação e época de amostragem nos teores dos nutrientes foliares na cultura do maracujazeiro amarelo (Passiflora edulis f. flavicarpa). O experimento foi conduzido em Campos-RJ, em Argissolo Amarelo distrófico, onde foram combinadas quatro doses de nitrogênio (50 a 650 g por planta por ano de N) com 6 lâminas de irrigação (0 a 125% da ETo - Evapotranspiração de referência). Na maior produtividade de frutos (41,3 t ha-1), os teores de nutrientes foliares variaram de 40,8 a 58,1 g kg-1 de N, 2,58 a 3,85 g kg-1 de P, 23,2 a 38 g kg-1 de K, 6,13 a 14,4 g kg-1 de Ca, 2,23 a 4,28 g kg-1 de Mg, 3,2 a 4,64 g kg-1 de S, 13,1 a 32,4 g kg-1 de Cl, 21,1 a 36,5 mg kg-1 de Zn, 44,4 a 94,5 mg kg-1 de Mn, 82 a 246 mg kg-1 de Fe, 16,8 a 48,9 mg kg-1 de B e 3,97 a 234 mg kg-1 de Cu. A adubação nitrogenada afetou os teores foliares de N, K, S, Ca, Mg, Mn, Cl e B. A irrigação elevou os teores foliares de Cl e de Na e não influenciou nos teores foliares dos outros nutrientes.
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Mbopi-Kéou, François-Xavier, Stefano Mion, Bertrand Sagnia, and Laurent Bélec. "Validation of a Single-Platform, Volumetric, CD45-Assisted PanLeucogating Auto40 Flow Cytometer To Determine the Absolute Number and Percentages of CD4 T Cells in Resource-Constrained Settings Using Cameroonian Patients' Samples." Clinical and Vaccine Immunology 19, no. 4 (February 15, 2012): 609–15. http://dx.doi.org/10.1128/cvi.00023-12.
Abstract:
ABSTRACTThe study evaluated the single-platform, volumetric, CD45-assisted PanLeucogating Auto40 flow cytometer (Apogee Flow Systems Ltd., Hemel Hempstead, United Kingdom) for CD4 T cell numeration, compared to the reference FACSCalibur flow cytometer. Results of absolute counts and percentages of CD4 T cells by Auto40 and FACSCalibur of 234 tripotassium EDTA (K3-EDTA)-blood samples from 146 adults and 88 children (aged from 18 months to 5 years), living in Yaoundé, Cameroon, were highly correlated (r2= 0.97 andr2= 0.98, respectively). The mean absolute bias and relative bias between Apogee Auto40 and FACSCalibur absolute CD4 T cell counts were +9.6 cells/μl, with limits of agreement from −251 to 270 cells/μl, and +4.1%, with limits of agreement from −16.1 to 24.4%, respectively. The mean absolute bias and relative bias between Apogee Auto40 and FACSCalibur CD4 T cell results expressed as percentages were +0.05% CD4 (95% confidence interval [CI], −0.03 to 0.41), with limits of agreement from −6.0 to 5.9% CD4, and +1.0%, with limits of agreement from −32.3 to 34.4%, respectively. The Auto40 counting allowed identification of the majority of adults with CD4 T cell counts below 200 cells/μl (sensitivity, 87%; specificity, 98%) or below 350 cells/μl (sensitivity, 92%; specificity, 98%) and of children with CD4 T cell counts below 750 cells/μl (sensitivity, 82%; specificity, 98%) or below 25% CD4+(sensitivity, 96%; specificity, 99%). The Auto40 analyzer is a reliable alternative flow cytometer for CD4 T lymphocyte enumeration to be used in routine immunological monitoring according to the WHO recommendations for HIV-infected adults as well as children living in resource-constrained settings.
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Menges, R. M., P. R. Nixon, and A. J. Richardson. "Light Reflectance and Remote Sensing of Weeds in Agronomic and Horticultural Crops." Weed Science 33, no. 4 (July 1985): 569–81. http://dx.doi.org/10.1017/s0043174500082862.
Abstract:
Plant canopy reflectance over the 0.45- to 1.25-μm wavelength (WL) of weed species and crops was recorded with a field spectroradiometer to evaluate the possible use of remote sensing to distinguish weeds from crops. Weed and weed-crop species reflectance differences were generally greater at the 0.85 μm WL in the near-infrared spectral region than at the 0.55 μm WL in the visible region, indicating that color infrared (CIR) aerial photography may be useful to detect weed populations in crops. Canopy reflectance data were more directly related to photographic differences in weed-crop images than were single leaf or inflorescence reflectance data. Aerial photography at altitudes of 610 to 3050 m distinguished climbing milkweed (Sarcostemma cyancboides♯ SAZCY) in orange [Citrus sinensis(L.) Osbeck. ‘Valencia’) trees; ragweed parthenium (Parthenium hysterophorusL. ♯ PTNHY) in carrot (Daucus carotaL., var.sativa‘Long Imperator’); johnsongrass [Sorghum halepense(L.) Pers. ♯ SORHA) in cotton (Gossypium hirsutumL. ‘CP 3774’) and in sorghum (Sorghum bicolorL. Moench. ‘Oro’); London rocket (Sisymbrium irioL. ♯ SSYIR) in cabbage; and Palmer amaranth (Amaranthus palmeriS. Wats. ♯ AMAPA) in cotton. Johnsongrass was also detectable with CIR film in maturing grain sorghum from 18 290 m. Detection of weed species in crops was aided by differential stages of inflorescence and senescence, and by the chlorophyll content, color, area, intercellular space, and surface characteristics of the leaves. Discrete plant community areas were determined by computer-based image analyses from a 1:8000-scale positive transparency with the efficiency of 82, 81, 68, and 100% for Palmer amaranth, johnsongrass, sorghum, and cotton, respectively. The computer analyses should permit discrete aerial surveys of weed-crop communities that are necessary for integrated crop management systems.
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Wigfield, Yuk Y., Mario D. Lacfloix, Monique Lanouette, and Narine P. Gurprasad. "Gas Chromatographic Determination of N-Nitrosodialkanolamines in Herbicide Di- or Trialkanolamine Formulations." Journal of AOAC INTERNATIONAL 71, no. 2 (March 1, 1988): 328–33. http://dx.doi.org/10.1093/jaoac/71.2.328.
Abstract:
Abstract A modified method is presented to determine trace quantities of N-nitrosodiethanolamine (NDE1A) and yV-nitrosodiisopropanolamine (NDiPlA) in the triisopropanolamine (TiPlA) formulation of a mixture of picloram and 2,4-D. Aqueous sample is extracted with dichloromethane to remove organic interferences, and then the aqueous layer is passed sequentially through chloride anion exchange column, hydrogen cation exchange column, and Clin-Elut extraction tube. The final eluate, 10% acetone in ethyl acetate, is concentrated. The isolated nitrosamines are converted to the corresponding trimethylsilyl (TMS) derivatives and determined by gas chromatography (GC) on a DB1 column coupled with a thermal energy analyzer (GC-TEA). Eight samples of commercial TiPlA formulations are analyzed. Maximum detected levels of NDE1A and NDiPlA were 0.6 and 0.9 ppm, respectively, expressed relative to total weight of active ingredients. Analysis of 13 samples of herbicide DEIA formulation using a previously established method and a DB225 column gave NDE1A results of 0.7-6.0 ppm. NDiPlA was not detected in those samples. Results are confirmed by GC-mass spectrometry (GC/MS) with oxygen negative chemical ionization (ONCI) detection. Dectection limits for both nitrosamines are 0.05 or 0.07 ng (0.1 or 0.17 ppm) for GC-TEA detection, depending on the analytical columns used, and 20 pg (0.04 ppm) for GC/MS detection. Recoveries of NDE1A are 87-109% for DEIA formulation spiked at 2.6 and 3.9 ppm and 90-115% for TiPlA formulation spiked at 0.2-0.3 ppm. Similarly, recoveries of NDiPlA are 95.7-100% for the DEIA formulation spiked at 0.24 and 0.48 ppm, and 82-118% for the TiPlA formulation spiked at 0.2-0.3 ppm.
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Sas, С. S., and S. A. Rudenko. "To study the prevalence of type II diabetes mellitus as a high–risk factor in patients with coronary heart disease undergoing coronary artery bypass grafting on a beating heart." Ukrainian Journal of Clinical Surgery 90, no. 4 (September 30, 2023): 11–15. http://dx.doi.org/10.26779/2786-832x.2023.4.11.
Abstract:
Objective. To study the prevalence of type II diabetes mellitus in patients with coronary heart disease who are to undergo coronary artery bypass grafting on a beating heart.
Materials and methods. The study included 991 patients: 786 (79.3%) men and 205 (20.7%) women aged 39 to 84 years, with a mean age of (64.3 ± 1.4) years. Men were aged 39 to 84 years, with a mean age of (62.7 ± 1.7) years, and women were aged 44 to 82 years, with a mean age of (66.0 ± 2.4) years. All study participants underwent coronary artery bypass grafting in the period from 2018 to 2021. The frequency of type II diabetes mellitus in different clinical groups of the study was analysed, taking into account its clinical course, compensation status and patient gender. Statistically, the data were processed using the χ² criterion with the Yates correction.
Results. It was found that the incidence of type II diabetes mellitus is 2.5 times higher in patients with coronary heart disease than in the general population. The prevalence of type II diabetes mellitus is statistically significantly higher in women with two and three or more coronary arteries.
Conclusions. The annual reports of the International Diabetes Federation and numerous scientific studies on type II diabetes mellitus prove that this pathology is extremely common, creates a risk of coronary heart disease, complicates its course, treatment and rehabilitation of patients. A statistical analysis of the prevalence of type II diabetes mellitus showed that in the cohort of patients to undergo coronary artery bypass grafting on a beating heart, it is statistically significantly more common in women with two and three or more coronary arteries affected.
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Videtic, G. M., C. A. Reddy, S. T. Chao, T. W. Rice, D. J. Adelstein, G. H. Barnett, T. M. Mekhail, M. A. Vogelbaum, and J. H. Suh. "Do gender and race influence survival in patients with non-small cell lung cancer brain metastases? An outcomes study utilizing the RTOG RPA class stratification." Journal of Clinical Oncology 24, no. 18_suppl (June 20, 2006): 7153. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.7153.
Abstract:
7153 Background: To explore gender, race and their interactions in the setting of NSCLC brain metastases only, a single-institution brain database was analyzed, using the RTOG recursive partitioning analysis (RPA) brain metastases classification. Methods: From 1/82 to 9/04, 831 NSCLC pts with brain metastases were registered. RPA criteria for analysis were: class I- Karnofsky performance status (KPS) ≥ 70, age<65 years, primary tumor controlled, no extracranial metastases; class III- KPS<70; class II- all others. Results: Median follow-up was 5.4 months (m) (range 0–122.9). Median age was 62.4 (range 25–90). Median KPS was 80 (range 20–100). There were 485 males [M] (58.4%) and 346 females [F] (41.6%). 824 pts (99%) were either African-American (AA; n = 142[17%]) or White (W; n = 682[83%]). Pts characteristics were balanced when stratified by RPA class and by treatments. Median survival (MS) in months from time of brain metastasis diagnosis for all pts was 5.8. MS in months by gender [F vs. M] and race [W vs. AA] was: 6.3 vs. 5.5, p = 0.013; 6.0 vs. 5.2, p = 0.08, respectively. By RPA class for gender, MS trends (in months) favored F over M in classes I and II but not III: 17.1 vs. 9.5 (p = 0.11); 6.8 vs. 6.0 (p = 0.09), 2.7 vs. 2.5 (p = 0.42), respectively. By RPA class for gender and race, MS trends (in months) favored AAF over AAM in classes I and II but not III: 30.0 vs. 12.4, p = 0.50; 11.2 vs. 4.6, p = 0.021; 3.2 vs. 3.2, p = 0.64, respectively; and WF over WM in classes I but not II or III: 14.4 vs. 9.5, p = 0.11; 6.6 vs. 6.3, p = 0.38; 2.4 vs. 2.3, p = 0.49, respectively. On multivariable analysis, significant variables were gender (p = 0.041; RR 0.83); RPA class (p < 0.0001; RR 0.28, for I vs. III; p < 0.0001; RR 0.51, for II vs. III). Conclusions: Gender significantly influences NSCLC brain metastasis survival while race trends to significance. MS trends by RTOG RPA class suggest race may interact with genderprimarily in class I but pt numbers limited significance. Further characterization of these factors is warranted. No significant financial relationships to disclose.
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Uvodich, Mason E., Zachary V. Braig, Anna K. Reinholz, Sara E. Till, Shawn W. O’Driscoll, Mark E. Morrey, Joaquin Sanchez-Sotelo, and Christopher L. Camp. "Incidence and Epidemiology of Symptomatic Capitellar Osteochondritis Dissecans of the Elbow: A United States Population–Based Study Over a 25-Year Period." Orthopaedic Journal of Sports Medicine 10, no. 11 (November 1, 2022): 232596712211359. http://dx.doi.org/10.1177/23259671221135933.
Abstract:
Background: There are limited data on the incidence of capitellar osteochondritis dissecans (OCD) in the United States (US) population. Purpose: To determine the incidence of symptomatic capitellar OCD in a representative US subpopulation and identify changes in its incidence over time and to investigate the relationship between the incidence of capitellar OCD and patient sex and age. Study Design: Descriptive epidemiology study. Methods: A retrospective review was performed of patients aged ≤24 years from Olmsted County, Minnesota, with symptomatic capitellar OCD over a 25-year period (1995-2019). Patients with acute osteochondral injuries, Panner disease, and hereditary arthropathy were excluded. Poisson regression was utilized to identify the predictors of a capitellar OCD diagnosis. Incidence rates (per 100,000) were assessed for changes over time. Age groups of younger (10-15 years) versus older (16-24 years) patients were compared. Results: A total of 45 patients (78% male) were identified. The mean age was 14.5 years (range, 10-24 years); 31 patients were in the younger group, and 14 patients were in the older group. Sport participation was seen in 89%of patients; 90% were overhead athletes, and 58% were throwing athletes. The incidence of capitellar OCD was 6.0 per 100,000 overall, 9.5 per 100,000 for male patients, and 2.6 per 100,000 for female patients. The incidence was highest for male patients in the younger group (15.3/100,000) and lowest for female patients in the older group (0.8/100,000). The estimated incidence rate ratio for younger versus older patients was 3.3 ( P < .001), and the incidence rate ratio for male versus female patients was 3.5 ( P < .001). There were no changes in the incidence of capitellar OCD over time as a continuous or 5-year categorical variable ( P = .290 and P = .460, respectively). Overall, 82% of patients were treated surgically. There were no significant changes in surgical rates over time. Conclusion: In this US subpopulation, the overall incidence of symptomatic capitellar OCD between the ages of 10 and 24 years was 6.0 per 100,000 over the 25-year period studied, which is higher than previously reported US estimates. Incidence rates and treatment strategies did not change significantly over time.