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1
Pilanc-Kudlek, Paulina, Katarzyna Poleszak, Aleksandra Ellert-Miklaszewska, Adria-Jaume Roura Canalda, Salwador Cyranowski, Julian Swatler, Bartłomiej Gielniewski, and Bożena Kamińska. "615 Reactivating antitumor immunity in gliomas with osteopontin/integrin blocking peptide." Journal for ImmunoTherapy of Cancer 9, Suppl 2 (November 2021): A645. http://dx.doi.org/10.1136/jitc-2021-sitc2021.615.
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BackgroundGlioblastoma (GBM) is the most common and aggressive primary brain tumor in adults. Despite improvements in imaging, surgical techniques, radiotherapy and chemotherapy, the prognosis of patients with GBM remains poor with a median overall survival of 15 months [1,2]. GBM is immunologically a ”cold” tumor with low infiltration of functional T and NK cells, which imposes poor responsiveness of GBM patients to immunotherapies. The immunosuppressive microenvironment in GBM is created by the malignant cells and tumor-associated macrophages (TAMs), such as resident brain microglia and recruited peripheral myeloid cells [3]. Osteopontin/Spp1 is one of glioma-derived factors that is responsible for the protumorigenic reprogramming of TAMs [4]. SPP1 expression is highly elevated in tumor tissues and sera from GBM patients, and inversely correlates with patient survival [5]. Cross-talk between malignant cells and TAMs relays on osteopontin binding to integrin receptors (mainly αvβ3 and αvβ5) via its RGD motif [6]. Thus, with the use of a RGD peptide (our in-house designed competitor of binding to integrins) we interfered with glioma-microglia interaction in vitro and evaluated the in vivo antitumor efficacy of integrin blockade as a monotherapy and in combination with an immune check-point inhibitor.MethodsThe efficacy of the RGD peptide to block microglia-dependent glioma invasion was determined in a Matrigel invasion assay. Antitumor activity of the peptide was assessed in a murine syngeneic orthotopic GL261 glioma model. RGD peptide was administrated intratumorally via osmotic pomps. For combination therapy, the animals received anti-PD-1 or isotype IgG antibody (4 inj. x 10 mg/kg i.p.). Tumor volume was measured using MRI. Heterogeneity of the immune cells compartment of glioma microenvironment was analysed by flow cytometry. The transcriptomes of CD11b+ cells immunosorted from tumor-bearing mouse brains were evaluated using RNAseq. Cytokine levels in the blood and the brain homogenates were measured using Luminex bead-based assays.ResultsThe microglia-stimulated invasion of GL261 glioma cells was reduced significantly in the presence of the RGD peptide in the in vitro co-culture system. The RGD peptide administrated to tumor-bearing mice induced proinflammatory reprogramming of TAMs. Combination of the RGD peptide with anti-PD-1 therapy increased the production of proinflammatory cytokines and the percentage of effector CD8+(CD44+CD62L-) cells in the tumors.ConclusionsThese results demonstrate that blockade of osteopontin/integrin signaling using the RGD peptide can mitigate the immunosuppressive microenvironment, reactivate the antitumor immunity and lay ground for improved response to immunotherapy in GBM.ReferencesJemal A, Murray T, Ward E, Samuels A, Tiwari RC, Ghafoor A, Feuer EJ, Thun MJ: Cancer statistics, 2005. CA Cancer J Clin 2005, 55(1):10–30.Stupp R, Hegi ME, Mason WP, van den Bent MJ, Taphoorn MJ, Janzer RC, Ludwin SK, Allgeier A, Fisher B, Belanger K et al: Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase III study: 5-year analysis of the EORTC- NCIC trial. Lancet Oncol 2009, 10(5):459–466.Woroniecka KI, Rhodin KE, Chongsathidkiet P, Keith KA, Fecci PE: T-cell Dysfunction in Glioblastoma: Applying a New Framework. Clin Cancer Res 2018, 24(16):3792–3802Denhardt, D.T., M. Noda, A.W. O’Regan, D. Pavlin, and J.S. Berman. 2001. Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival. J Clin Invest 107:1055–1061.Grassinger, J., D.N. Haylock, M.J. Storan, G.O. Haines, B. Williams, G.A. Whitty, et al. 2009. Thrombin-cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with alpha9beta1 and alpha4beta1 integrins. Blood 114:49–59.Anborgh, P.H., J.C. Mutrie, A.B. Tuck, and A.F. Chambers. 2010. Role of the metastasis-promoting protein osteopontin in the tumour microenvironment. Journal of cellular and molecular medicine 14:2037–2044Ethics ApprovalAll research protocols conformed to the Guidelines for the Care and Use of Laboratory Animals (European and national regulations 2010/63/UE September 22, 2010 and Dz. Urz. UE L276/20.10.2010, respectively). Animals were decapitated by a qualified researcher. The First Warsaw Local Ethics Committee for Animal Experimentation approved the study (approval no. 812/2019).
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Le Bourgeois, Amandine, Thierry Guillaume, Jacques Delaunay, Pierre Peterlin, Viviane Dubruille, Beatrice Mahe, Nicolas Blin, et al. "Important Prognostic Impact of Early Monocytes Recovery after Reduced Intensity Conditioning Double Umbilical Cord Blood Allogeneic Stem Cell Transplantation in Adults." Blood 124, no. 21 (December 6, 2014): 5923. http://dx.doi.org/10.1182/blood.v124.21.5923.5923.
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Abstract Introduction: Little is known regarding the impact of hematopoietic and immune recoveries after double umbilical cord blood (dUCB) allogeneic stem cell transplantation (allo-SCT), especially after the TCF (low dose 2 Grays total body irradiation + cyclophosphamide 50 mg/Kg 1 day + fludarabine 200 mg/m² 5 days) reduced-intensity conditioning (RIC) regimen, which is considered as a standard RIC regimen for dUCB allo-SCT in adults Patients and Methods: Here we considered a homogeneous cohort of 47 patients (males: n=24; median age: 55.5 years (range: 17.5-69) who engrafted after a dUCB TCF allo-SCT performed between November 2006 and April 2013 in our department. Fifty-three percent of the patients had myeloid disease. The majority of cases were in complete remission at time of transplant (72.3%). GVHD prophylaxis consisted of cyclosporine + mycophenolate mofetyl in all cases. All patients received G-CSF from day 1 until neutrophils recovery. The median nucleated cells dose infused was 4.17 107/kg. The aim of the study was to investigate the impact on outcomes of the recovery of the following cellular subsets: leucocytes, monocytes, lymphocytes, neutrophils at day +30 and day +42, and CD4+, CD8+ T cells, B and NK cells at day+100. Results: Median times for neutrophils and platelets recoveries were 17 days (range: 6-59) and 37 days (range: 0-164), respectively. With a median follow-up of 30.4 months (range: 2.8-77.5), the 3-year overall and relapse-free survivals (OS, RFS), relapse incidence (RI), and non-relapse mortality (NRM) were 65.7%, 57.2%, 27.1% and 19%, respectively. The cumulative incidences of grade II-IV and grade III-IV acute GVHD were 38.3% and 10.6%, respectively, while, 3-year incidence of chronic GVHD was 53.5% (limited 42%, extensive 11.5%). In univariate analysis, 3-year OS was significantly higher in case of lymphoid disease (80.9% vs 51.9%, p=0.05) or when achieving at day+30 or day +42 higher counts of leucocytes (> median: 2760/mm3; 79% vs 51%, p=0.05; median > 4250/mm3; 78.6% vs 55.4%, p=0.04) or monocytes (> median: 615/mm3; 87.5% vs 45.8 %, p=0.02; median > 830/mm3, 86.2% vs 54.1%, p=0.03). Older age (>median: 55 years) and higher monocytes count at day +42 (> median: 830/mm3) were significantly associated with higher 3-year RFS (63.6% vs 49.1 %, p=0.046; and 75.7 vs 44.4%, p=0.014). Higher leucocytes count at day +42 (>median: 4250/mm3) was the only factor associated with significant 3-year lower NRM (7.1% vs 31.7%, p=0.04), while younger age was associated with higher risk of grade 3-4 acute GVHD (16.7% vs 4.4 %, p=0.05). No factor was predictive of chronic GVHD in this series. In multivariate analysis, older age and early higher monocytes count after transplant were the two independent factors associated with a significantly higher OS (>55 years, HR: 0.21; 95%CI: 0.05-0.85, p=0.028; >615/mm3 at day +30, HR: 0.05; 95%CI: 0.01-0.43, p=0.006) while only older age remained independently associated with better RFS (>55 years, HR: 0.25, 95%CI: 0.08-0.78, p=0.017). No factor was predictive of NRM, grade 2-4 GVHD, grade III-IV acute or chronic GVHD. Conclusion: These results suggest that higher early monocytes recovery is predictive of outcome after dUCB TCF RIC allo-SCT in adults. Immune recovery seems to have no impact on survivals in this series while influence of age has to be confirmed by other studies. Our results pave the way for future studies aiming to closely and prospectively monitor the kinetics of hematopoietic and immune recoveries after this type of graft. As all patients received G-CSF after transplant, other immunostimulatory cytokines should be tested to ensure sufficient hematopoietic recovery in the setting of adult dUCB TCF RIC allo-SCT. Disclosures No relevant conflicts of interest to declare.
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Curtin, John, Martin Cormican, Gerard Fleming, John Keelehan, and Emer Colleran. "Linezolid Compared with Eperezolid, Vancomycin, and Gentamicin in an In Vitro Model of Antimicrobial Lock Therapy for Staphylococcus epidermidis Central Venous Catheter-Related Biofilm Infections." Antimicrobial Agents and Chemotherapy 47, no. 10 (October 2003): 3145–48. http://dx.doi.org/10.1128/aac.47.10.3145-3148.2003.
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ABSTRACT Central venous catheter (CVC)-related infection (CVC-RI) is a common complication of CVC use. The most common etiological agents of CVC-RI are gram-positive organisms, in particular, staphylococci. An in vitro model for the formation of biofilms by Staphylococcus epidermidis ATCC 35984 on polyurethane coupons in a modified Robbins device was established. Biofilm formation was confirmed by electron microscopy and was quantified by determination of viable counts. Mueller-Hinton broth was replaced with sterile physiological saline (control) or a solution of vancomycin (10 mg/ml), gentamicin (10 mg/ml), linezolid (2 mg/ml), or eperezolid (4 mg/ml). Viable counts were performed with the coupons after exposure to antimicrobials for periods of 24, 72, 168, and 240 h. The mean viable count per coupon following establishment of the biofilm was 4.6 × 108 CFU/coupon, and that after 14 days of exposure to physiological saline was 2.5 × 107 CFU/coupon. On exposure to vancomycin (10 mg/ml), the mean counts were 2.5 × 107 CFU/coupon at 24 h, 4.3 × 106 CFU/coupon at 72 h, 1.4 × 105 CFU/coupon at 168 h, and undetectable at 240 h. With gentamicin (10 mg/ml) the mean counts were 2.7 × 107 CFU/coupon at 24 h, 3.7 × 106 CFU/coupon at 72 h, 8.4 × 106 CFU/coupon at 168 h, and 6.5 × 106 CFU/coupon at 240 h. With linezolid at 2 mg/ml the mean counts were 7.1 × 105 CFU/coupon at 24 h and not detectable at 72, 168, and 240 h. With eperezolid (4 mg/ml) no viable cells were recovered after 168 h. These data suggest that linezolid (2 mg/ml) and eperezolid (4 mg/ml) achieve eradication of S. epidermidis biofilms more rapidly than vancomycin (10 mg/ml) and gentamicin (10 mg/ml).
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Pereira, Ricardo B., Agnaldo DF Carvalho, Jadir B. Pinheiro, Giovani O. Silva, and Jairo V. Vieira. "Avaliação de híbridos experimentais de cenoura no Distrito Federal." Horticultura Brasileira 33, no. 1 (March 2015): 34–39. http://dx.doi.org/10.1590/s0102-053620150000100006.
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O objetivo deste trabalho foi avaliar o potencial agronômico de 24 híbridos de cenoura, sendo nove obtidos pelo cruzamento entre linhagens de origem tropical (grupo Brasília: CNPH-520 e CNPH-536) e temperada (macho-estéreis: CNPH-610, CNPH-664, CNPH-633, CNPH-712 e CNPH-671) e 15 obtidos do cruzamento entre linhagens de origem tropical (CNPH-1 a CNPH-15) e a linhagem macho-estéril CNPH-612. Entre 2011 e 2012 foram realizados dois experimentos em blocos casualizados com três repetições, em que a parcela experimental consistiu de três fileiras duplas espaçadas por 20 cm, com 10 cm entre linhas. A queima-das-folhas ocorreu de forma natural no campo e foi avaliada 90 dias após a semeadura. Os rendimentos agronômicos foram avaliados 100 dias após a semeadura. No primeiro experimento os híbridos CNPH-610×CNPH-536, CNPH-712×CNPH-536 e CNPH-610×CNPH-520 apresentaram menores severidades da doença, enquanto CNPH-610×CNPH-520 e os híbridos CNPH-664×CNPH-520 e CNPH-671×CNPH-520 apresentaram as maiores produções de raízes comerciais e menores produções de raízes refugo. Os híbridos não diferiram entre si em relação ao peso e comprimento médio de raízes. No segundo experimento, os híbridos CNPH-612×CNPH-2, CNPH-612×CNPH-6, CNPH-612×CNPH-10 e CNPH-612×CNPH-11 apresentaram resultados superiores para todas as características avaliadas (resistência à queima-das-folhas, produção de raízes comerciais e refugo, peso, comprimento e diâmetro médio de raízes), seguidos de CNPH-612×CNPH-3, que apresentou inferior comprimento médio de raízes.
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Nilla, Saima Sharif, Md Anisur Rahman Khan, Md Mahmudur Rahman Khan, Dewan Ali Ahsan, and Md Ghulam Mustafa. "Bacteriological Quality of Marketed Mola Fish, Amblypharyngodon Mola From Dhaka Metropolis." Bangladesh Journal of Zoology 40, no. 1 (December 9, 2012): 77–88. http://dx.doi.org/10.3329/bjz.v40i1.12897.
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The bacteriological quality of mola fish (Amblypharyngodon mola) from three local fish markets as fresh and as frozen from three departmental chain shops of Dhaka metropolis were analyzed. The microbial quality parameters varied with different sources and the quality was found to be poor for local market fish samples. In total 24 samples were considered for bacteriological quality analysis and 10 pathogenic isolates for antibiotic sensitivity test to 12 antibiotics. The total bacterial count ranged from 1.8 ± 0.25×104 to 6.5 ± 0.75 × 106 cfu/g for fresh and 5.5 ± 0.55 × 103 to 7.0 ± 0.80 × 105 cfu/g for frozen mola. The highest total coliform count of mola was 8.0 ± 0.55 × 104 and 6.1 ± 0.40 × 103 cfu/g for local market and departmental chain shop, respectively. All fresh and frozen samples were observed having high quantity of E. coli above 102 cfu/g. Furthermore, Salmonella-Shigella was identified in 67% samples (75% of fresh and 58% of frozen samples) varied from 0.9 ± 0.00 ×102 to 5.3 ± 0.30 × 103 cfu/g whereas Vibrio spp.was confirmed in 79% samples (83% of fresh and 75% of frozen samples) of which 90% samples exceeded 102 cfu/g. Similar pattern was observed in Staphylococcus spp. with 83% of fresh and 58% of frozen samples (63% of total samples) beyond 103 cfu/g. In case of antibiotic sensitivity pattern of the indicator and pathogenic isolates, all of them were resistant to amoxicillin and penicillin. Most of the isolates were sensitive to bacitracin, ciprofloxacin, erythromycin and streptomycin. The findings proved that mola fish under this study was more or less contaminated and local fish market samples were highly privileged with food borne pathogens which confirmed the unhygienic condition of the market as well as the presence of antibiotic resistance bacteria in mola fish may threat to public health safety. DOI: http://dx.doi.org/10.3329/bjz.v40i1.12897 Bangladesh J. Zool. 40(1): 77-88, 2012
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Emmett, Louise, Sarennya Pathmanandavel, Andrew Nguyen, Megan Crumbaker, Andrew On Wah Yam, Wai Ling Chan, Karen Fullard, Bao Ho, Arun Azad, and Anthony M. Joshua. "Results of a phase I/II prospective dose-escalation trial evaluating safety and efficacy of combination 177LuPSMA-617 and NOX66 in men with mCRPC post androgen signalling inhibition and two lines of taxane chemotherapy (LuPIN trial)." Journal of Clinical Oncology 38, no. 6_suppl (February 20, 2020): 120. http://dx.doi.org/10.1200/jco.2020.38.6_suppl.120.
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120 Background: Despite treatment advances, metastatic castrate resistant prostate cancer (mCRPC) remains a lethal disease. Trials in 177LuPSMA-617 have demonstrated good efficacy and safety, but synergistic combinations may further improve treatment responses. NOX66 inhibits external NADH oxidase type 2 with downstream pro-apoptotic actions including radio-sensitization. We present results of a prospective open label single arm phase 1/2 dose escalation/expansion trial of 177LuPSMA-617 and NOX66 in mCRPC. Methods: Men with progressive mCRPC post androgen signalling inhibition (ASI) and taxane chemotherapy were eligible. Inclusion criteria included PSMA PET/CT intensity > SUV max 15, with no discordant disease on FDG PET/CT, Hb > 100 g/L, Platelets > 90 x 106/L and GFR > 40 mL/min. Protocol allowed up to 6 doses of 177 Lu-PSMA 617 (7.5Gbq) on day 1 with NOX66 (suppository) given day 1-10 at 6-weekly intervals; the first 8 men received 400mg NOX66. After safety review, dose was escalated to 800mg. Data regarding safety, efficacy, pain scores, and QOL were collected. Results: 32/43 (26% imaging screen failures) screened men were enrolled (November 2017 – June 2019), of whom 100% had prior docetaxel and ASI, and 94% (30/32) cabazitaxel. All men received ≥ 2 cycles, with 12/32 completing 6 cycles, and 16/32 2 - 5 cycles, while 4/32 remain on treatment. Any PSA response was seen in 84% (27/32), with a PSA response > 50% in 62.5% (20/32). Median PSA PFS was 6.5 months (95%CI 3.54-9.3). To date, 72% (23/32) of patients have progressed. 34% (11/32) men have died with median OS not reached. 50% (12/24) of men with baseline pain scores ≥3 (24/32) had significant reduction in pain. Adverse events are summarized below. Conclusions: Combination 177LuPSMA-617 with NOX66 appears safe and efficacious in men with heavily pre-treated mCRPC. Clinical trial information: ACTRN12618001073291. [Table: see text]
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Gray, James X., Lyle McMillen, Russell Saal, Steven Lane, Peter Mollee, Robert Bird, Devinder Gill, and Paula Marlton. "WT1 Expression Levels at Diagnosis and as a Marker of Minimal Residual Disease (MRD) in Patients with Acute Myeloid Leukaemia (AML)." Blood 114, no. 22 (November 20, 2009): 2639. http://dx.doi.org/10.1182/blood.v114.22.2639.2639.
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Abstract Abstract 2639 Poster Board II-615 Background.- As wt1 expression is elevated in the majority (80%) of acute myeloid leukemias at diagnosis and relapse, a validated wt1 assay would be a powerful adjunctive tool for evaluation and monitoring of patients during their treatment. Monitoring of disease status with wt1 is applicable to more AML patients in a single assay than any other leukemic marker and includes patients that are cytogenetically normal and may not otherwise be amenable to molecular MRD evaluation. We aimed to analyse wt1 expression in the peripheral blood and bone marrow of patients with de novo AML, when measured at diagnosis, post-induction and post-consolidation; and to correlate these wt1 expression levels with patient characteristics and outcome. Methods- This is a prospective, longitudinal study in which the levels of wt1 expression from 99 patients with de novo AML are measured from bone marrow (59 patients) and peripheral blood (94 patients) specimens at the time of diagnosis and at various time-points along their treatment and clinical courses. The specimens were prospectively stored in the PwC Australasian Leukaemia and Lymphoma Group (ALLG) Tissue Bank. Samples analysed included: 59 bone marrow (bm-wt1) and 94 peripheral blood (pb-wt1) at diagnosis; 54 bm-wt1 and 57 pb-wt1 post-induction; and 46 bm-wt1 and 41 pb-wt1 post-consolidation. 90 of the 99 patients were treated with standard induction chemotherapy on a variety of protocols. RNA was extracted from 107 leukocytes purified from blood and bone marrow using TRIzol® (Invitrogen) extraction. Reverse transcription was performed using Superscript ® (Invitrogen) and RQ-PCR was performed using multiplexed 5' nuclease assay with Black Hole Quencher labelled probes according to published methods. Copy number of wt1 was normalised to 104 copies of ABL. Baseline patient characteristics were correlated with baseline wt1 expression levels and the impact of baseline and post-treatment wt1 expression on leukemia-free survival (LFS) was assessed. Results- 99 AML patients were studied: median age 55years, 53% male. Cytogenetic prognostic groups (SWOG) were favourable – 12%, intermediate – 59% and poor – 29%. 90 patients were treated with curative intent and 83% achieved CR, 6% had refractory disease and 11% died during induction. With a median follow-up of 33 months, the median LFS was 12 months and median OS was 24 months. Median pb-wt1 levels at diagnosis were 5122 (range, 1 to 36125) and varied significantly according to: cytogenetic risk group (good risk 12745, intermediate risk 2632, poor risk 4554 (P = 0.009)) and marrow blast percentage (P = 0.02). Diagnostic bm-wt1 showed similar correlations with baseline parameters, but did not reach statistical significance. Median pb-wt1 at relapse was 3781. One of 21 relapsed patients with elevated pb-wt1 expression at diagnosis did not express wt1 at relapse. In a multivariate Cox regression analysis model including age and cytogenetic risk-group, increased expression of wt1 from peripheral blood at diagnosis is predictive of decreased LFS (P=0.04). This correlation was not seen with wt1 measured from bone marrow aspirates. The wt1 expression levels were reduced following induction chemotherapy with a median 2.6 log reduction (range, 0 to 4.2) seen in marrow aspirate and 3.2 log reduction (range, 0 to 4.3) seen in peripheral blood. There was a trend for lower post-induction peripheral blood wt1 to correlate with better LFS when measured as a continuous variable (P=0.067) or undetected vs detected (P=0.051). Lower post-consolidation pb-wt1 significantly correlated with better LFS when measured as a continuous variable (P=0.002) or undetected vs detected (P=0.02) and continued to be significant in multivariate analysis. Bone marrow aspirate wt1 levels post-induction or post-consolidation did not correlate with LFS. Conclusion- This study demonstrates a correlation between wt1 expression levels and known risk factors for early relapse. In the MRD setting, detectable wt1 levels in peripheral blood post-induction and post-consolidation predicted very poor leukaemia free survival. Analysis is ongoing to validate these findings in a uniformly treated cohort of patients enrolled on the current ALLG clinical trial. Disclosures: No relevant conflicts of interest to declare.
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Vinha, Mariana Barboza, Cláudia Lúcia de Oliveira Pinto, and José Benício Paes Chaves. "Estafilococos coagulase positiva em queijos Minas Frescal produzidos em agroindústrias familiares." Revista do Instituto de Laticínios Cândido Tostes 73, no. 2 (November 13, 2018): 62–72. http://dx.doi.org/10.14295/2238-6416.v73i2.656.
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O objetivo deste estudo foi quantificar Estafilococos Coagulase Positiva (ECP) em queijos Minas Frescal, produzidos em seis queijarias não inspecionadas, sendo que destas, três delas utilizam leite cru e três utilizam leite pasteurizado. Foram coletadas 78 amostras, sendo 46 (24 na agroindústria e 22 no comércio) produzidas com leite pasteurizado e 32 (15 na agroindústria e 17 no comércio) fabricadas com leite cru. Queijos produzidos com leite pasteurizado apresentaram baixas contagens de ECP (1,0 x 101 a 2,0 x 103 UFC/g) e menor percentual de amostras não conformes (6,5%), as quais podem ser justificas por contaminações pós-pasteurização, decorrente de falhas higiênico-sanitárias durante a fabricação e comercialização do produto. Queijos fabricados com leite cru apresentaram maior contaminação (6,0 x 102 a 3,6 x 106 UFC/g) e maior percentual de amostras não conformes (75,0%), podendo estas serem atribuídas à contaminação da matéria-prima. Queijos informais são potencialmente capazes de causar gastroenterite e oferecem risco à saúde pública. Agroindústrias familiares devem cumprir as exigências sanitárias a fim de oferecer produtos seguros aos consumidores, evitar surtos, regularizar a produção e ampliar a comercialização, proporcionando o desenvolvimento e consolidação desta importante atividade no meio rural.
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Bassett, D. J., E. Bowen-Kelly, and S. S. Reichenbaugh. "Rat lung glucose metabolism after 24 h of exposure to 100% oxygen." Journal of Applied Physiology 66, no. 2 (February 1, 1989): 989–96. http://dx.doi.org/10.1152/jappl.1989.66.2.989.
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Previous studies with lung homogenates and isolated cells have suggested oxygen cell injury results from the inhibition of key enzymes involved in both cytosolic and mitochondrial energy generation. In this study, the extent and pattern of metabolism of D-[U-14C, 5–3H]glucose was examined in perfused lungs isolated from rats before and after 24 h of in vivo exposure to 100% O2. Lung ATP levels after O2 exposure were maintained by a 53% increase in glucose utilization from an unexposed control value of 18.0 +/- 3.2 to 27.5 +/- 3.0 mumol 3H2O.h-1.g dry wt-1, accounted for by an enhanced rate of lactate plus pyruvate production from 15.7 +/- 2.0 to 32.7 +/- 4.1 mumol.h-1.g dry wt-1 with no alteration in lactate-to-pyruvate ratio. CO2 production was unaltered from a control rate of 27.5 +/- 4.0 14CO2 mumol.h-1.g dry wt-1. Maximal rates of glucose metabolism were determined by perfusion with 0.8 mM dinitrophenol, giving for air-exposed lungs a rate of 53.5 +/- 5.0 mumol 3H2O.h-1.g dry wt-1 and increased lactate plus pyruvate and 14CO2 production rates of 46.5 +/- 6.5 and 128.3 +/- 19.6 mumol.h-1.g dry wt-1, respectively. Although this maximal rate of glucose utilization was unaltered in oxygen-exposed lungs, lactate plus pyruvate production was further increased to 80.0 +/- 9.1 mumol.h-1.g dry wt-1 with a concomitant decrease in the dinitrophenol-induced rate of 14CO2 production to 81.5 +/- 9.2 mumol.h-1.g dry wt-1.(ABSTRACT TRUNCATED AT 250 WORDS)
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Demir, Ibrahim, Canan Oztokat Kuzucu, Sıtkı Ermis, and Güleda Öktem. "Radicle Emergence as Seed Vigour Test Estimates Seedling Quality of Hybrid Cucumber (Cucumis sativus L.) Cultivars in Low Temperature and Salt Stress Conditions." Horticulturae 9, no. 1 (December 20, 2022): 3. http://dx.doi.org/10.3390/horticulturae9010003.
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This work was conducted on the use of the RE (radicle emergence) test to estimate the seedling emergence (SE) and quality (seedling shoot weight (SSW), seedling root weight (SRW) and seedling height (SH)) of twelve hybrid cucumber seed lots with high germination (>95%) at low temperature conditions, high salt conditions and a combination of the two. The percentages of RE, which is a 2 mm radicle protrusion at 25 °C in the dark, were counted between 18 h and 32 h. The seeds were sown in peat moss in trays and kept at 15 °C (low temperature stress), or salty water was applied as 100 mM NaCl at 25 °C (salt stress) for three days, and both were applied in a combined-stress environment. Seedling emergence and quality parameters were reduced by both stress environments and their combination. SE, SSW, SRW and SH values ranged between: 91–100%, 614–844 mg, 102–143 mg, 6.8–8.8 cm at low temperature; 90–98%, 598–904 mg, 101–154 mg, 6.5–7.8 cm at salt stress; and 76–92%, 464–608 mg, 97–133 mg, 5.8–6.9 cm at their combination. The RE values with great differences seen among the lots (20–28 h) were regressed with seedling emergence and quality values. RE 24 h had the highest R2 and significant values in all stress environments as R2 = 0.596-0.858, p < 0.05–0.001 at low temperature; R2 = 0.620–0.827, p < 0.05–0.001 with salt; and R2 = 0.686-0.842, p < 0.05–0.001 with combined stresses. We concluded that RE as a vigour test can be used successfully to estimate the seedling quality of highly germinating hybrid cucumber seed lots. Use of the RE test in hybrid cucumber seeds and its influence on high-quality seedling production were discussed.
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Taguchi, Fumiaki, Jun Dan Chang, Naoki Mizukami, Tatsuo Saito-Taki, Katsushige Hasegawa, and Masayoshi Morimoto. "Isolation of a hydrogen-producing bacterium, Clostridium beijerinckii strain AM21B, from termites." Canadian Journal of Microbiology 39, no. 7 (July 1, 1993): 726–30. http://dx.doi.org/10.1139/m93-105.
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Clostridium beijerinckii strain AM21B was isolated from termites. At uncontrolled pH, it produced 108.9 mmol of hydrogen in 24 h from 10 g of glucose at a conversion rate of 105.7 mmol/g dry cells, and it produced 101.6 mmol of hydrogen in 24 h from 10 g of starch at pH 6.0, at a conversion rate of 77.6 mmol/g dry cells at pH 7.0. The hourly maximum hydrogen evolution rates were 22.3 mmol/h from glucose and 18.3 mmol/h from starch at pH 6.5 and 36 °C. Strain AM21B also produced hydrogen from arabinose, cellobiose, fructose, galactose, lactose, sucrose, and xylose at conversion efficiencies ranging from 15.7 to 19.0 mmol/g of substrate for 24 h.Key words: hydrogen production, carbohydrates, bacteria, termites, Clostridium beijerinckii.
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Lim, Chaemoon, Chang Ho Shin, Won Joon Yoo, and Tae-Joon Cho. "Acute correction of proximal tibial coronal plane deformity in small children using a small monolateral external fixator with or without cross-pinning." Journal of Children's Orthopaedics 15, no. 3 (June 1, 2021): 255–60. http://dx.doi.org/10.1302/1863-2548.15.200187.
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Purpose Surgical correction of proximal tibia deformity in small children can be challenging. We present the surgical technique and outcome of proximal tibia osteotomy fixed with small monolateral external fixator in this patient group. Methods A total of 17 cases in eight patients younger than nine years of age were study subjects. A proximal tibia osteotomy was fixed with a small monolateral external fixator with or without cross-pinning. Outcome was evaluated by changes of radiographic parameters such as medial proximal tibia angle (MPTA), metaphyseal diaphyseal angle (MDA) and clinical findings of complications, time interval until weight bearing and fixator removal time. Results MPTA improved from a preoperative mean of 73° (sd 4°; 66° to 78°) to an immediate postoperative mean of 90° (sd 3°; 85° to 96°) in varus tibiae, and from 104° (sd 1°; 103° to 105°) to 89° (sd 1°; 88° to 89°) in valgus tibiae. In all, 15 of the 17 cases (88.3 %) achieved postoperative MPTA within the normal range (85° to 90°). MDA improved from a preoperative mean of 19° (sd 5°; 11° to 24°) to an immediate postoperative mean of 0° (sd 4°; -6° to 7°) in varus tibiae, and from -25° (sd 2°; -22° to -24°) to 2° (SD 1°; 1° to 3°) in valgus tibiae. Full weight bearing was possible at mean 1.7 months (0.5 to 3.0). Mean follow-up period was 6.5 years (sd 5.4; 1.0 to 16.0). No complications developed during the follow-up. Conclusion Proximal tibia osteotomy fixed with small monolateral external fixator provides accurate, safe and efficient correction in the management of coronal plane angular deformity in small children. Level of Evidence Level IV
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Credito, Kim, Genrong Lin, and Peter C. Appelbaum. "Antistaphylococcal Activity of DX-619 Alone and in Combination with Vancomycin, Teicoplanin, and Linezolid Assessed by Time-Kill Synergy Testing." Antimicrobial Agents and Chemotherapy 51, no. 4 (January 29, 2007): 1508–11. http://dx.doi.org/10.1128/aac.01517-06.
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ABSTRACT Time-kill synergy studies testing in vitro activity of DX-619 alone and with added vancomycin, teicoplanin, or linezolid against 101 Staphylococcus aureus strains showed synergy between DX-619 and teicoplanin at 12 to 24 h in 72 strains and between DX-619 and vancomycin in 28 strains. No synergy was found with linezolid, and no antagonism was observed with any combination.
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Nikitin, Kirill D., Sergei B. Fitilev, Tatiana V. Chernovskaya, Elena G. Rudenko, Alexander V. Vozzhaev, Yulia Y. Titarova, Vadim A. Yakushev, and Roman A. Ivanov. "Pharmacokinetics, pharmacodynamics, and tolerability of BCD-017, a novel pegylated filgrastim: Results of open-label controlled phase I study with dose escalation in healthy volunteers." Journal of Clinical Oncology 30, no. 15_suppl (May 20, 2012): 9060. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.9060.
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9060 Background: Pegfilgrastim (conjugate of filgrastim and 20 kDa PEG) is approved for treatment of chemotherapy-associated neutropenia. BCD-017 is a covalent conjugate of filgrastim with 30 kDa PEG. Increased molecular weight of PEG molecule may provide additional benefits compared to pegfilgrastim. We have conducted this open-label phase I study to assess the PK, PD and tolerability of BCD-017. Methods: 24 healthy male volunteers signed the informed consent and were sequentially assigned to receive 1, 3 or 9 mg of BCD-017 or 5 mcg/kg/day of filgrastim for 7 days, 6 volunteers per group. Outcome measures included absolute neutrophil count (ANC) and СD34+ cell count, PK parameters and adverse events (AEs). Results: BCD-017 induced a fast and significant increase of ANC. Median maximum ANC (ANCmax) for BCD-017 1, 3, 9 mg and filgrastim was 18.68 (10.62-21.02), 25.92 (15.43-28.07), 32.22 (18.22-45.79), and 28.21 (21-31.95) ×103 cells/mm3, respectively; median time to ANCmax was 24 (12-24); 48 (24-72); 72 (48-72); and 132,5 (12-169) h, respectively; median increase in СD34+ cells number 96 h post dose was 4.7 (1.2-6.5), 6.5 (1.7-12.3), 40.9 (24.5-102), and 17.8 (3.3-35.2) times, respectively. Filgrastim serum concentration was analyzed using ELISA. Median Cmax for BCD-017 3 and 9 mg and filgrastim was 45 (31-65), 446 (191-649), and 40 (20-54) ng/mL, respectively; median Tmax was 48 (24-72), 36 (24-48), and 8 (6-8) h respectively; median T1/2 was 65 (51-70), 46 (41-57), and 6.7 (6.2-7.6) h, respectively. BCD-017 was well tolerated. No dose-limiting AEs were observed. AEs included headache, back pain, myalgia, arthralgia, thrombocytopenia, hyperuricemia, alkaline phosphatase/LDH increased. All AEs were of grade 1-2. Compared to filgrastim, the best tolerability was observed in 3 mg group. Conclusions: BCD-017 is shown to be a potent stimulator of granulopoiesis. BCD-017 3 mg did not differ from filgrastim in terms of ANC increase and its safety was shown in healthy volunteers. Further phase II study of BCD-017 for treatment and prophylaxis of neutropenia in patients receiving cytotoxic chemotherapy is necessary.
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Swanson, Analicia J., Kiah M. Gourley, Cassandra K. Jones, Joel M. DeRouchey, Mike D. Tokach, Steve S. Dritz, Robert D. Goodband, and Brent Frederick. "361 Increasing duration of feeding high dietary lysine and energy before farrowing on colostrum quality and yield in mixed parity sows." Journal of Animal Science 97, Supplement_2 (July 2019): 150–51. http://dx.doi.org/10.1093/jas/skz122.266.
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Abstract A total of 472 mixed parity sows were used from d 106 of gestation until weaning to determine the effects of Lys and energy intake in the last 7 d before farrowing on colostrum production, composition, and immunoglobulin G concentrations. On d 106 of gestation, sows were weighed, blocked by parity and weight, and allotted to dietary treatments. Treatments were based on corn-soybean meal diets fed to provide 1) 12.5 g SID Lys and 6.5 Mcal/d ME from d 107 to 112 of gestation, then 28 g SID Lys and 9.4 Mcal/d ME from until farrowing; 2) 12.5 g SID Lys and 6.5 Mcal/d ME from d 107 to 112 of gestation, then 40 g SID Lys and 13.3 Mcal/d ME from until farrowing; 3) 40 g SID Lys and 13.3 Mcal/d ME from d 107 of gestation until farrowing. After birth of the first piglet, a 50 mL colostrum sample was collected. Colostrum samples were analyzed for fat, protein, total solids, lactose, and IgG concentrations. Individual piglets were weighed at birth and 24 h to determine colostrum intake and yield. Data was analyzed for treatment within parity and treatment main effects. There was a treatment within parity difference observed where sows on treatment 3 had decreased (P < 0.05) fat and total solids concentrations compared to treatment 1 sows, with no evidence for difference within gilts. IgG concentrations tended to increase (P = 0.081) in females fed treatment 2 compared to treatment 1, regardless of parity. Total protein and lactose concentrations as well as colostrum intake and yield were similar across treatments, regardless of parity. In conclusion, feeding high Lys and energy for 7 d before farrowing decreased fat and solid concentrations in colostrum in sows, but did not impact other colostrum nutrients or colostrum production.
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Kuo, John, Omid R. Hariri, Galyna Bondar, Julie Ogi, and Paul Micevych. "Membrane Estrogen Receptor-α Interacts with Metabotropic Glutamate Receptor Type 1a to Mobilize Intracellular Calcium in Hypothalamic Astrocytes." Endocrinology 150, no. 3 (October 23, 2008): 1369–76. http://dx.doi.org/10.1210/en.2008-0994.
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Estradiol, acting on a membrane-associated estrogen receptor-α (mERα), induces an increase in free cytoplasmic calcium concentration ([Ca2+]i) needed for progesterone synthesis in hypothalamic astrocytes. To determine whether rapid estradiol signaling involves an interaction of mERα with metabotropic glutamate receptor type 1a (mGluR1a), changes in [Ca2+]i were monitored with the calcium indicator, Fluo-4 AM, in primary cultures of female postpubertal hypothalamic astrocytes. 17β-Estradiol over a range of 1 nm to 100 nm induced a maximal increase in [Ca2+]i flux measured as a change in relative fluorescence [ΔF Ca2+ = 615 ± 36 to 641 ± 47 relative fluorescent units (RFU)], whereas 0.1 nm of estradiol stimulated a moderate [Ca2+]i increase (275 ± 16 RFU). The rapid estradiol-induced [Ca2+]i flux was blocked with 1 μm of the estrogen receptor antagonist ICI 182,780 (635 ± 24 vs. 102 ± 11 RFU, P < 0.001) and 20 nmof the mGluR1a antagonist LY 367385 (617 ± 35 vs. 133 ± 20 RFU, P < 0.001). Whereas the mGluR1a receptor agonist (RS)-3,5-dihydroxyphenyl-glycine (50 μm) also stimulated a robust [Ca2+]i flux (626 ± 23 RFU), combined treatment of estradiol (1 nm) plus (RS)-3,5-dihydroxyphenyl-glycine (50 μm) augmented the [Ca2+]i response (762 ± 17 RFU) compared with either compound alone (P < 0.001). Coimmunoprecipitation demonstrated a direct physical interaction between mERα and mGluR1a in the plasma membrane of hypothalamic astrocytes. These results indicate that mERα acts through mGluR1a, and mGluR1a activation facilitates the estradiol response, suggesting that neural activity can modify estradiol-induced membrane signaling in astrocytes. For rapid 17β-estradiol-induced membrane signaling in hypothalamic astrocytes, mER-α must interact with mGluR1a resulting in a dramatic increase in free cytoplasmic calcium concentration within seconds.
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Patrizio, Imperiali, Ralli Chiara, Duranti Diletta, Clienti Carla, Liberti Maria Elena, Selvi Antonio, Sciri Raffaela, et al. "Possible effect of dialysis membrane in polymethylmethacrylate on clinical variables associated with atherosclerosis development in chronic renal failure patients." Archives of Renal Diseases and Management 7, no. 1 (January 4, 2022): 001–5. http://dx.doi.org/10.17352/2455-5495.000038.
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Background: Chronic hemodialysis patients have higher cardiovascular morbidity compared to the general population. A number of studies have suggested that patients undergoing hemodialysis with polymethylmethacrylate (PMMA) membranes have a better outcome compared to other membranes. Methods: We performed a retrospective, multicenter study to evaluate the impact of PMMA membranes compared to other types of membranes on clinical parameters considered important risk factors for the development of cardiovascular disease in chronic kidney disease patients. Results: The study included 104 patients (52 patients on PMMA and 52 patients on other membranes) from ten dialysis centers, monitored for 24 months. HDL cholesterol (mg/dL) increased significantly in the PMMA group (41.4 ± 10.8 to 44.1 ± 13.5, p = 0.0467), but not in the control group (41.8 ± 13.8 to 39.4 ± 9.6, p = 0.8628). At 24 months total cholesterol and triglycerides (mg/dl) were significantly lower in the PMMA group than in the control group (142.4 ± 43.8 vs. 166.1 ± 43.4, p = 0.0321 and 106 (76.5-176) vs. 170 (118-254), p = 0.014), respectively. Serum creatinine (mg/dL) increased significantly from baseline to 24months in the PMMA group (9.20 ± 2.5 to 9.47 ± 2.3, p = 0.0291), but not in the patients treated with other membranes (8.39 ± 2.6 to 8.37 ± 2.3, p = 0.2743). In addition creatinine was significantly higher in the PMMA group compared to the other group (9.47 ± 2.3 vs. 8.37 ± 2.3, p = 0.0493). WBCs (109/L) increased significantly in the control group (6151 ± 1846 to 6672 ± 1872, p = 0.0457) but not in the PMMA group (6326 ± 2113 to 6152 ± 1832, p = 0.8981). At 24 months platelets (109/L) and CRP (ng/dL) were significantly lower in the PMMA group compared to the control group (185 (144-222) vs. 210 (173-259), p = 0.0498 and 0.70 (0.30-1.59) vs. 3.76 (0.46-10.2), p = 0.023, respectively). Iron and transferrin (μ g/dL) decreased signifi cantly in the patients treated with other membrane (62.5 ± 30.4 to 52.6 ±19.0, p = 0.0113 and 178 (157-218) to 170 (124-203), p = 0.0019, respectively), but not in the PMMA group. Conclusion: This retrospective study of data from 104 patients shows a favorable effect of PMMA on clinical variables considered relevant for the development of atherosclerosis in hemodialysis patients.
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Jiao, P. X., Z. X. He, S. Ding, N. D. Walker, Y. Y. Cong, F. Z. Liu, K. A. Beauchemin, and W. Z. Yang. "Impact of strain and dose of live yeast and yeast derivatives on in vitro ruminal fermentation of a high-grain diet at two pH levels." Canadian Journal of Animal Science 98, no. 3 (September 1, 2018): 477–87. http://dx.doi.org/10.1139/cjas-2017-0079.
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The objective of this study was to determine the effects of live yeast (LY) or yeast derivatives (YD) on gas production (GP), dry matter (DM) disappearance (DMD), fermentation characteristics, and microbial profiles in batch culture. The study was a completely randomized design with a factorial arrangement: 2 media pH × 5 yeasts × 4 dosages. An additional treatment of monensin (Mon) was added as a positive control for each pH level. Media pH was low (5.8) and high (6.5); the yeasts were three LY (LY1-3) and two YD (YD4-5); and doses were 0, 4 × 106, 8 × 106, and 1.6 × 107 cfu mL−1 for LY and 0, 15, 30, and 60 mg bottle−1 for YD. Substrate consisted of 10% silage and 90% concentrate (DM basis) and samples were incubated for 24 h. Media pH of 6.5 vs. 5.8, increased (P < 0.01) GP, DMD, and volatile fatty acid (VFA) concentrations but decreased (P < 0.01) NH3-N concentration and copy numbers of Ruminococcus albus, Ruminococcus flavefaciens, and Selenomonas ruminantium. Increasing dose of LY2 linearly (P < 0.05) increased DMD. Total VFA concentration was greater with LY2 (P < 0.01) than LY3 and YD5 at pH 6.5. Overall, adding yeast products improved (P < 0.05) DMD at pH 5.8, and increased VFA concentration compared with Mon. These results indicate that in vitro GP and DMD of a high-grain diet varied with source and dose of yeast supplementation. Some yeast products have the potential to improve fermentation of feedlot diets when supplemented at appropriate doses.
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Reece, P. A., I. Stafford, R. L. Abbott, C. Anderson, J. Denham, S. Freeman, R. G. Morris, P. G. Gill, and C. L. Olweny. "Two- versus 24-hour infusion of cisplatin: pharmacokinetic considerations." Journal of Clinical Oncology 7, no. 2 (February 1989): 270–75. http://dx.doi.org/10.1200/jco.1989.7.2.270.
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The disposition of unchanged cisplatin was compared after two- and 24-hour intravenous (IV) infusion to eight patients with germ cell cancer (dose, 100 mg/m2), 14 patients with head and neck cancer (dose, seven patients 50 mg/m2; seven patients, 100 mg/m2). Patients were randomized to receive either a two- or 24-hour infusion in the first course of treatment and the reverse in the second course. Cisplatin renal clearance, total clearance, and the percentage of the dose excreted unchanged in urine were significantly lower with the longer infusion. Total clearance was 345 +/- 97.0 mL/min/m2 after the two-hour infusion and 268 +/- 70.7 mL/min/m2 after the 24-hour infusion (P less than .0001). Renal clearance was 79.1 +/- 35.3 mL/min/m2 and 34.1 +/- 14.9 mL/min/m2 (P less than .0001). The percentage of the dose excreted unchanged in urine was 22.9 +/- 6.5% and 12.8 +/- 4.0%, respectively (P less than .0001). The ratio of cisplatin renal clearance to creatinine clearance was 1.95 +/- .96 after the two-hour infusion and .90 +/- .40 after the 24-hour infusion (P less than .001). There was only a poor relationship between cisplatin renal clearance and creatinine clearance after a two-hour infusion (r2 = .05, P greater than .1) or 24-hour infusion (r2 = .18, P greater than .05). The severity of emesis was graded on a four-point scale and was significantly less with the 24-hour infusion than with the two-hour infusion (P less than .05). Twenty-four-hour infusion of cisplatin resulted in greater drug retention in patients due to reduced renal clearance, but was also associated with reduced emetic toxicity, probably as a result of lower peak plasma levels.
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Busnach, G., A. dal Col, B. Brando, M. L. Perrino, C. Brunatti, and L. Minetti. "Efficacy of a Combined Treatment with Plasma Exchange and Cytostatics in Macroglobulinemia." International Journal of Artificial Organs 9, no. 4 (July 1986): 267–70. http://dx.doi.org/10.1177/039139888600900412.
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Monthly plasma exchange (PE) sessions have been carried out in 3 patients with advanced Waldenström macroglobulinemia, in order to reduce electrophoretic M band under 2g/100 ml. When PE was combined to low doses of cytostatics (n=1 8), 3 procedures per session were required to obtain a mean 57.4±12.3% IgM reduction, from 4.2±1.2 to 1.7±0.5 g/100 ml. A mean 61.5±13.1% IgM reduction, from 5.5±1.3 to 2.1±1 g/100 ml, was obtained in 64 procedures carried out as the only therapy in 12 sessions, with 5.3 procedures requirement per monthly session. IgM percent reduction 24 hours after PE was greater with combined treatment (45±9.7 vs. 28.9±1 5.4%; p=0.001). The advantage of a combined treatment is therefore either a lowered PE requirement or a tapered maintenence cytostatic dosage.
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Falkinham, Joseph O. "Growth in catheter biofilms and antibiotic resistance of Mycobacterium avium." Journal of Medical Microbiology 56, no. 2 (February 1, 2007): 250–54. http://dx.doi.org/10.1099/jmm.0.46935-0.
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Cells of Mycobacterium avium strain A5 adhered to plasticized polyvinyl chloride catheter tubing and grew at low nutrient concentration, consistent with reports of catheter-associated M. avium infection. Starting with initial cell densities of 1–2×106 c.f.u. ml−1, biofilms of approximately 350 c.f.u. cm−2 formed within 24 h at room temperature. Growth rates of cells in biofilms were exponential and equal to 2.45 days doubling time. Rates were exponential for 1–2 weeks incubation and reached cell densities of 6.5×104 c.f.u. cm−2 by 4 weeks. Cells grown in catheter biofilms were significantly more resistant to clarithromycin and rifamycin than cells grown in suspension.
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North, Frederick, Elissa M. Nelson, Rebecca J. Majerus, Matthew C. Thompson, Aric J. Knutson, and Brian A. Crum. "Self-Scheduling Process Efficiency and Utilization of Online Self-Scheduling of Lab Tests: A Retrospective Analysis of Self-Scheduled Appointments for COVID Testing." Health Services Research and Managerial Epidemiology 9 (January 2022): 233339282211250. http://dx.doi.org/10.1177/23333928221125034.
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Introduction The COVID 19 pandemic increased the need for rapid and accurate diagnostic testing for COVID. When testing became available, a systems response was needed to efficiently accommodate the high-volume flow of patients who needed testing. Self-scheduling of COVID testing was developed to help patients safely and efficiently schedule their COVID testing online or with a mobile app. Methods We captured the counts of COVID test appointments, time patients spent in scheduling COVID test appointments, appointment lead times, and no-shows for COVID test appointments. For 17 months of self-scheduling, we retrospectively compared self-scheduling with the concurrent staff scheduling of COVID tests. Results From November 2020 through March 2022 there were 619 104 scheduled appointments for COVID testing with 22% (136 252) being self-scheduled. For asymptomatic self-scheduled COVID tests, accounting for 10.3% (63 605/619 104) of total COVID tests scheduled, median time to self-schedule was 3.1 min, interquartile range (IQR) [2.4,4.7]. For symptomatic self-schedulers accounting for 11.7% (72 647/619 104) of total COVID tests scheduled, the median time to self-triage and self-schedule was 5.8 min, IQR[4.3,8.9]. Self-scheduled COVID appointments increased to 44% (42 387/97 086) of the total COVID appointments during the peak month of January 2022. Median appointment lead time for symptomatic self-scheduled COVID test appointments was 6.6 h compared to 2.9 h ( P < .0001) for symptomatic staff scheduled appointments. However, adjusting for the 24% (32 194/135 252) that self-scheduled during hours when testing was unavailable, the median appointment lead time for symptomatic self-scheduled patients dropped to 3.6 h. No-shows were 2.5% for self-scheduled appointments compared to 3.0% no-shows that were staff scheduled (odds ratio 0.83, P < .0001). Conclusion COVID testing was self-scheduled for a large percent of scheduled COVID tests, taking patients only a few minutes to complete. Self-scheduling use increased over time, associated with a decreasing use of staff scheduled appointments and lower no-shows.
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Tsisaryk, O., І. Slyvka, and L. Musiy. "Скринінг технологічних властивостей природних штамів молочнокислих бактерій." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 19, no. 80 (October 8, 2017): 88–92. http://dx.doi.org/10.15421/nvlvet8018.
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У статті висвітлені результати досліджень технологічних властивостей молочнокислих бактерій (МКБ). Проведено скринінг 106 культур МКБ виділених із традиційної бринзи виготовленої у непромислових умовах із овечого молока у Карпатському регіоні України. При досліджені технологічних властивостей МКБ враховували ферментативну і кислотоутворювальну активність штамів на 3,6,9 та 24 год. культивування, розраховували кількість молочної кислоти, визначали здатність до росту мікроорганізмів за різних температурних режимів +10 °С,+15 °С,+45 °С та стійкість культур мікроорганізмів до різних концентрацій NaCl – 2,4 і 6,5%. В результаті проведених досліджень встановлено, що ріст за температури +10 °С і +15 °С та концентрації NaCl 2 і 4% був властивий для більшості лактобактерій. Відзначено активний ріст і розвиток штамів Enterococcus faecium за температури +45°С і концентрації NaCl 6,5%.
Щодо кислотоутворювальної здатності, встановлено, що найактивнішим був вид Lactococcus lactis ssp. lactis з енергією кислотоутворення 80–100 °Т. Найменш вираженою здатність до утворення молочної кислоти характеризувались штами виду Lactococcusgarvieae та Enterococcus faecium 60–80 °Т.
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Nguyen, Chau N., Anh T. Do, Phuc K. Hoang, and Lam X. Truong. "Pathogenicity of four entomopathogenic nematode strains against Asian corn borer, Ostrinia furnacalis (Guenée), in Vietnam." Nematology 20, no. 8 (2018): 729–36. http://dx.doi.org/10.1163/15685411-00003171.
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Summary The virulence and reproductive capacity of four indigenous entomopathogenic nematode (EPN) strains, S-PQ16 (Steinernema sp. PQ16), S-TX1 (Steinernema sangi TX1), S-DL13 (S. siamkayai DL13) and H-NT3 (Heterorhabditis indica NT3), against the last instar larvae of Asian corn borer (ACB), was evaluated. With moderately low concentrations of infective juveniles (IJ) from 10 to 100 IJ insect−1, the ACB larvae were found to be susceptible to all four EPN nematode strains after exposure for 24, 48, 72, 96 and 120 h. The 100% mortality of ACB larvae by these indigenous EPN strains occurred after 96-120 h of exposure. The low LC50 values (between 21.7 and 27.9 IJ) indicated the high virulence of these four EPN strains to ACB larvae. The highest yield of IJ produced inside the insect cadavers for S-PQ16, S-TX1, S-DL13 and H-NT3 was 215.0 ± 6.5 × 103, 83.1 ± 4.9 × 103, 67.8 ± 7.2 × 103 and 225.2 ± 6.4 × 103 IJ, respectively, with low inoculated concentrations of between 30-50 IJ. The results, showing a high degree of virulence and high reproduction capacity, suggest that these indigenous EPN strains have great potential for biological control of the Asian corn borer.
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Shen, Yijun, Xiaolin Lu, Xuejun Ma, Wei Liu, and Dingwei Ye. "Preliminary efficacy and safety results from ReBirth: A phase II study of risk-based bladder-sparing therapy for MIBC." Journal of Clinical Oncology 42, no. 4_suppl (February 1, 2024): 615. http://dx.doi.org/10.1200/jco.2024.42.4_suppl.615.
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615 Background: Trimodal therapy (TMT) has achieved long-term survival and persistent oncologic control in selected MIBC patients, however, tailored treatment using biomarkers based on chemotherapy plus PD-1 inhibitor responses is currently absent. Furthermore, the safety and efficacy of hypo-fractionated radiation in combination with PD-1 inhibitors and concurrent chemotherapy is worth exploring. Methods: This is a two-stage, single-arm, phase II trial recruiting cT2-4aN0-1M0 MIBC pts. Based on results of cystoscopy, urine cytology, imaging and MRD detection after first stage (Tislelizumab (T) 200 mg on D1, Cisplatin (C) 70 mg/m2 on D1 and Gemcitabine (G) 1000 mg/m2 on D1 and D8 Q3W for 3-4 cycles), pts achieving cCR (cT0, cTa) are treated with T, while the other pts receive T and chemoradiotherapy (whole bladder 44Gy/16 fractionation combined with C as radiosensitizer). 1-year BIDFS rate is the primary endpoint. Secondary endpoints include 2-year MFS rate, 2-year BIDFS rate and safety. Tissue and urine samples will be obtained for genetic profiling and biomarker research. Results: The preliminary efficacy and safety are reported this time. As of September 20, 2023 (median follow up: 258 (49-415) days, 25 pts with a median age of 64 (36-77) years were enrolled and 96% are male. 19 pts with cT2 (57.14%), cT3 (33.33%) and cT4 (9.52%) tumors were evaluable. 2 pts were assessed as N1. 14/19 pts (73.68%) achieved cCR and maintained a sustained response. Based on positive urine cytology and MRD, 5 pts were classified as non-cCR (2 pts in cT3N0M0, 2 pts in cT4N0M0 and 1 pt in cT2N0M0). Due to RC (2 pts achieving ypT0N0 and 1 pt achieving ypT2N0) and incomplete treatment cycles, 6 pts were excluded from the efficacy analysis set. BFS rate at 1 yr is evaluated in 6 pts (2 pts received hypo-fractionated on the second stage) and the rate is 100%. TRAEs were found in 19 of 25 pts (76%). The grade 3-4 TRAEs observed (8%) was adrenal cortical insufficiency and immuno-therapy related colonitis. Hematological AEs (32%), renal insufficiency (24%), pruritus (20%), and fatigue (20%) are the most common AEs. During hypo-fractionated radiation, no new safety signs were discovered. Conclusions: The preliminary findings indicate a potential efficacy and manageable toxicity during the two-stage treatment. Enrollment is still ongoing, and long-term efficacy will be proved. Clinical trial information: NCT05531123 .
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Ricaud, Philippe, Massimo Del Guasta, Angelo Lupi, Romain Roehrig, Eric Bazile, Pierre Durand, Jean-Luc Attié, Alessia Nicosia, and Paolo Grigioni. "Supercooled liquid water clouds observed over Dome C, Antarctica: temperature sensitivity and cloud radiative forcing." Atmospheric Chemistry and Physics 24, no. 1 (January 17, 2024): 613–30. http://dx.doi.org/10.5194/acp-24-613-2024.
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Abstract. Clouds affect the Earth climate with an impact that depends on the cloud nature (solid and/or liquid water). Although the Antarctic climate is changing rapidly, cloud observations are sparse over Antarctica due to few ground stations and satellite observations. The Concordia station is located on the eastern Antarctic Plateau (75∘ S, 123∘ E; 3233 m above mean sea level), one of the driest and coldest places on Earth. We used observations of clouds, temperature, liquid water, and surface irradiance performed at Concordia during four austral summers (December 2018–2021) to analyse the link between liquid water and temperature and its impact on surface irradiance in the presence of supercooled liquid water (liquid water for temperature less than 0 ∘C) clouds (SLWCs). Our analysis shows that, within SLWCs, temperature logarithmically increases from −36.0 to −16.0 ∘C when liquid water path increases from 1.0 to 14.0 g m−2. The SLWC radiative forcing is positive and logarithmically increases from 0.0 to 70.0 W m−2 when liquid water path increases from 1.2 to 3.5 g m−2. This is mainly due to the downward longwave component that logarithmically increases from 0 to 90 W m−2 when liquid water path increases from 1.0 to 3.5 g m−2. The attenuation of shortwave incoming irradiance (that can reach more than 100 W m−2) is almost compensated for by the upward shortwave irradiance because of high values of surface albedo. Based on our study, we can extrapolate that, over the Antarctic continent, SLWCs have a maximum radiative forcing that is rather weak over the eastern Antarctic Plateau (0 to 7 W m−2) but 3 to 5 times larger over West Antarctica (0 to 40 W m−2), maximizing in summer and over the Antarctic Peninsula.
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Gilmartin, G. S., M. Lynch, R. Tamisier, and J. W. Weiss. "Chronic intermittent hypoxia in humans during 28 nights results in blood pressure elevation and increased muscle sympathetic nerve activity." American Journal of Physiology-Heart and Circulatory Physiology 299, no. 3 (September 2010): H925—H931. http://dx.doi.org/10.1152/ajpheart.00253.2009.
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Chronic intermittent hypoxia (CIH) is thought to be responsible for the cardiovascular disease associated with obstructive sleep apnea (OSA). Increased sympathetic activation, altered vascular function, and inflammation are all putative mechanisms. We recently reported (Tamisier R, Gilmartin GS, Launois SH, Pepin JL, Nespoulet H, Thomas RJ, Levy P, Weiss JW. J Appl Physiol 107: 17–24, 2009) a new model of CIH in healthy humans that is associated with both increases in blood pressure and augmented peripheral chemosensitivity. We tested the hypothesis that exposure to CIH would also result in augmented muscle sympathetic nerve activity (MSNA) and altered vascular reactivity contributing to blood pressure elevation. We therefore exposed healthy subjects between the ages of 20 and 34 yr ( n = 7) to 9 h of nocturnal intermittent hypoxia for 28 consecutive nights. Cardiovascular and hemodynamic variables were recorded at three time points; MSNA was collected before and after exposure. Diastolic blood pressure (71 ± 1.3 vs. 74 ± 1.7 mmHg, P < 0.01), MSNA [9.94 ± 2.0 to 14.63 ± 1.5 bursts/min ( P < 0.05); 16.89 ± 3.2 to 26.97 ± 3.3 bursts/100 heartbeats (hb) ( P = 0.01)], and forearm vascular resistance (FVR) (35.3 ± 5.8 vs. 55.3 ± 6.5 mmHg·ml−1·min·100 g tissue, P = 0.01) all increased significantly after 4 wk of exposure. Forearm blood flow response following ischemia of 15 min (reactive hyperemia) fell below baseline values after 4 wk, following an initial increase after 2 wk of exposure. From these results we conclude that the increased blood pressure following prolonged exposure to CIH in healthy humans is associated with sympathetic activation and augmented FVR.
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Hsiao, Ting-Jung, Po-Han Chiu, Cheng-Lin Tai, Tzu-Ching Tsao, Chien-Yu Tseng, Yi-Xian Lin, Hsueh-Ren Chen, et al. "Effect of Cu Additions on the Evolution of Eta-prime Precipitates in Aged AA 7075 Al–Zn–Mg–Cu Alloys." Metals 12, no. 12 (December 9, 2022): 2120. http://dx.doi.org/10.3390/met12122120.
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In the present study, after solid solution treatment, four different artificial aging treatments (100, 120, 140 and 160 °C) were performed on Al-5.98Zn-2.86Mg-1.61Cu (wt.%) alloy, denoted as 7075-LCu, and Al-5.91Zn-2.83Mg-1.98Cu (wt.%) alloy, denoted as 7075-HCu. Peak aging conditions were determined for each aging temperature at various hold time intervals of up to 24 h. It was found that both alloys possessed the optimal strengths after artificial aging at 120 °C for 24 h. Under this condition, the ultimate tensile strengths (UTSs) were 618 MPa (7075-LCu) and 623 MPa (7075-HCu), respectively. Moreover, a method was used to calculate the average sizes and number density of the major strengthening precipitates, η′, under peak aging conditions in these two alloys from transmission electron microscopy (TEM) images and electron energy loss spectroscopy (EELS). The above results indicated that for the 7075-LCu and 7075-HCu samples with the optimal UTS strengths, the former possessed an average thickness of 2.15 nm, and a number density of 3.27 × 1017 cm−3; the latter, 2.04 nm and 3.52 × 1017 cm−3.
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Engel-Nitz, Nicole M., Stacey DaCosta Byfield, Timothy Bancroft, Amy J. Anderson, Carolina M. Reyes, Arliene Ravelo, Sarika Ogale, Matthew J. Matasar, and May C. Chen. "Understanding disease progression and treatment patterns in metastatic breast, colorectal, and lung cancer: Implications for evaluating value and quality of care." Journal of Clinical Oncology 34, no. 7_suppl (March 1, 2016): 24. http://dx.doi.org/10.1200/jco.2016.34.7_suppl.24.
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24 Background: Impact of strategies for evaluating value and quality of cancer care may vary for patients with metastatic tumors, particularly for strategies considering lines of treatment therapy as distinct events. To aid in evaluating the value and quality of care in patients’ overall trajectory of disease, this study examined disease progression, mortality, hospice, and treatment patterns in patients (pts) with metastatic breast (mBC), lung (mLC), and colorectal cancers (mCRC). Methods: Included were commercially insured and Medicare Advantage adults from a large US health plan administrative claims database (2007-2014); pts had ≥ 2 claims for BC, CRC, or LC, and ≥ 2 claims for metastases. Index date was defined as the first post-metastatic systemic anti-cancer therapy (TX). Health plan enrollment for 6 mo pre- and post-index date was required; pts with < 6 mo of follow-up due to death were included. Pts with other primary cancers were excluded. A line of therapy (LOT) algorithm was developed and outcomes assessed over a variable follow-up. Progressive disease (PD) was defined as: start of a new LOT, where 1) previous LOT duration > 60 days, and 2) ≥ 1 imaging/lab test occurred between previous LOT and new LOT; receipt of hospice care; or death. Results: Among 7070 mBC, 4767 mCRC and 6994 mLC patients, 73%, 72%, and 84% respectively progressed during the study period (incidence rates: 7, 7, and 13 per 10-pt-years). Mean (median) time in months to PD was 9.5 (6.5) for mBC, 9.0 (7.0) for mCRC and 6.3 (5.0) for mLC. For all cancers, median number of LOT over 1 year was 2, with means of 1.8 (mBC), 1.7 (mCRC), and 1.7 (mLC). Compared to patients without PD, patients with PD had shorter LOT1. Mortality was highest among mLC patients (50%) compared to mCRC (27%) and mBC (22%), with rates per 100-pt-years of 50, 16, and 11 respectively. Hospice rates varied by tumor type. Conclusions: The high level of progression and use of multiple lines of therapy in the metastatic setting suggests a need to delay progression with effective treatments for breast, lung, and colorectal cancers. The value and quality of cancer care should be evaluated across the entire span of patients’ disease.
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Al-Sayyed, Hiba, Abdelmnim M. Altwaiq, Leen N. Ali, and Sa’ib Khouri. "Screening for the Presence of Some Heavy Metals, Total Soluble Solids and Caffeine Contents in Some Energy Drinks Sold in Jordanian Market." Methods and Objects of Chemical Analysis 19, no. 1 (2024): 20–24. http://dx.doi.org/10.17721/moca.2024.20-24.
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Energy drinks (EDs) are beverages that are devoid of alcohol containing high amounts of caffeine and sugar. Energy drinks are available in the Jordanian market and consumed by Jordanian youth. There is no Jordanian standard for EDs. Establishing a standardization for a certain contaminant requires assessing the heavy metal content in the EDs available in the market. This research aimed to screen the 10 most consumed energy drinks sold in the Jordan market for total soluble solids, caffeine contents, and the presence of some heavy metals. This study, thus, will serve as an initial step for setting a standard for heavy metals in EDs in Jordan. The analyzed EDs didn’t contain detectable amounts of Pb. On the other hand, the analyzed samples contained 0.1 to 2.8, 1.8 to 6.5, 0.01, and 0.2 to 3.5 mg kg-1 Cu, Ni, Cd, and Fe respectively. The total soluble solids content of the studied samples ranged between 4.8 to 15.4 g/100 g. On the other hand, the caffeine content of the analyzed samples ranged between 24.7 to 32 mg/100 mL. It is recommended to establish a Jordanian standards specification for energy drinks including heavy metal, total dissolved solids, and caffeine.
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Kramp, R., P. Fourmanoir, and N. Caron. "Endothelin resets renal blood flow autoregulatory efficiency during acute blockade of NO in the rat." American Journal of Physiology-Renal Physiology 281, no. 6 (December 1, 2001): F1132—F1140. http://dx.doi.org/10.1152/ajprenal.0078.2001.
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First published August 15, 2001; 10.1152/ajprenal.00078.2001.—Renal blood flow (RBF) autoregulatory efficiency may be enhanced during NO inhibition in the rat, as recently reported. Under these conditions, endothelin (ET) synthesis and release may be increased. Our purpose was therefore to determine the role of ET in RBF autoregulatory changes induced by NO inhibition. To address this point, ETA/B receptors were blocked in anesthetized rats with bosentan, or selectively with BQ-610 or BQ-788. NO synthesis was inhibited with N G-nitro-l-arginine methyl ester (l-NAME). Mean arterial pressure (MAP) was decreased after bosentan (−10 mmHg; P < 0.01) or increased after l-NAME (25 mmHg; P < 0.001). RBF measured with an electromagnetic flow probe was reduced byl-NAME (−50%) and by BQ-788 (−24%). The pressure limits of the autoregulatory plateau (PA ∼100 mmHg) and of no RBF autoregulation (Po ∼80 mmHg) were significantly lowered by 15 mmHg after l-NAME but were unchanged after bosentan, BQ-610, or BQ-788. During NO inhibition, autoregulatory resetting was completely hindered by bosentan (PA ∼100 mmHg) and by ETB receptor blockade with BQ-788 (PA ∼106 mmHg), but not by ETA receptor blockade with BQ-610 (PA ∼85 mmHg). These results suggest that the involvement of ET in the RBF autoregulatory resetting occurs during NO inhibition, possibly by preferential activation of the ETB receptor. However, the relative contribution of ET receptor subtypes remains to be further specified.
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Pathmanandavel, Sarennya, Megan Crumbaker, Andrew On Wah Yam, Christopher Rofe, Bao Ho, Wai Ling Chan, Shikha Sharma, et al. "Final results of a phase I/II prospective dose escalation trial evaluating safety and efficacy of combination 177Lu PSMA 617 and NOX66 in men with end-stage metastatic castration-resistant prostate cancer (LuPIN trial)." Journal of Clinical Oncology 39, no. 6_suppl (February 20, 2021): 103. http://dx.doi.org/10.1200/jco.2021.39.6_suppl.103.
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103 Background: 177LuPSMA – 617 is a promising therapy for metastatic castrate-resistant prostate cancer (mCRPC). However, treatment resistance occurs frequently and synergistic combination therapy may improve outcomes. We combined 177LuPSMA – 617 with idronoxil (NOX66), an inhibitor of external NADH oxidase type 2 with radio-sensitizing properties. We present the final safety and efficacy results. Methods: Men with progressive mCRPC after androgen signalling inhibition (ASI) and taxane chemotherapy were enrolled. Key inclusion criteria were: PSMA PET/CT intensity SUV max > 15 with no discordant disease on FDG PET/CT, haemoglobin > 100g/L, platelets > 100x109/L and eGFR > 40mls/min. Enrolled patients received up to six doses of 177 Lu-PSMA 617 (7.5Gbq) day 1 every 6 weeks in combination with NOX66 days 1-10 each cycle. Cohort 1 (n = 8) received 400mg NOX66. Following safety reviews the doses were escalated in cohorts 2 (n = 24) and 3 (n = 24) to 800mg and 1200mg of NOX66, respectively. Blood samples were prospectively collected for androgen receptor splice variant 7 (ARV7) expression. PSMA and FDG PET/CT were performed at study entry and on progression. The primary outcomes were safety and tolerability; the secondary outcomes evaluated were efficacy, pain scores, and quality of life. Results: Of the 56 men enrolled, all had received prior treatment with ASI and docetaxel, and 95% (53/56) had prior cabazitaxel. 96% (54/56) patients received ≥2 cycles and 46% (26/56) completed six cycles of treatment. Adverse events are summarized in the table below. PSA responses were as follows: 86% (48/56) had any PSA reduction and 61% (34/56) had > 50% PSA reduction. 84% (47/56) have had PSA progression to date with median follow up 18.9 months (95% CI 11.9-25.8). Median PSA PFS was 7.5 months (95% CI 6.0-9.0). 55% (31/56) have died and median overall survival was 19.7 months (95% CI 10.7-28.7). 34/56 men had baseline pain scores ≥3, of whom 53% (18/34) had significant reduction in pain indicators. There was no correlation between quantitative PET results and either PSA > 50% response, PSA PFS or OS. Conclusions: The combination of 177 Lu-PSMA 617 + NOX66 appears safe and efficacious in men with heavily pre-treated mCRPC. Exploratory analysis of ARV7 expression and quantitative PET imaging markers of treatment response and resistance is in progress. Clinical trial information: ACTRN12618001073291. [Table: see text]
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Morsheda, Hamima Umme, Md Nahid Uz Zaman, and Nahid Afroze. "Assessment of job satisfaction among the senior staff nurses working at Sadar Hospital, Naogaon, Bangladesh." Asian Journal of Medical and Biological Research 2, no. 4 (January 23, 2017): 611–15. http://dx.doi.org/10.3329/ajmbr.v2i4.31004.
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This study aimed to examine nurses? job satisfaction at Sadar Hospital, Naogaon, Bangladesh. It also examines the relationships between nurses? assessment, satisfaction, attitude, and practice with respect to job satisfaction. The descriptive cross sectional study design was used to explore the job satisfaction among the staff nurses. Total number of nurses working in this hospital was 121. A total number of fifty nurses were recruited into this study in order to overcome non-response subjects. The age group 30-39 years were 40%, 40-49 years were 46% and 50+ above were 14% nurses. Male nurses were only 6% and the female were 94%. Marital status single 12%, married 80%, widow 8%. Muslim nurses were 58%, Hindu 38% and Christian 4%. Academic qualification of them was SSC 66% and H.S.C 34%. Professional qualification was Diploma in Nursing /Diploma in Midwifery 92%, B.Sc. in public Health Nursing 6% and MPH 2%. Length of service 1 to 10 years 22%, 11 to 20 years 50%, 21 to 25 years 24%, 31+ years 4%. Regarding maintaining therapeutic relationship; facing problem in duty; keeping records; receiving complicated patient; attend in duty time; maintaining aseptic techniques; maintaining work; carryout admission and discharge the results for all above questions were 100% yes. But regarding satisfy to give care 30% respondent were not satisfied; whole for job salary 68% nurses were not feel enough and remaining 32% were satisfied. Again in case of promotion to the next post most of the respondent 16% were satisfied and 84% were not satisfied.Asian J. Med. Biol. Res. December 2016, 2(4): 611-615
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Yukawa, Hiroshi, Hirofumi Noguchi, Koichi Oishi, Soichi Takagi, Michinari Hamaguchi, Nobuyuki Hamajima, and Shuji Hayashi. "Cell Transplantation of Adipose Tissue-Derived Stem Cells in Combination with Heparin Attenuated Acute Liver Failure in Mice." Cell Transplantation 18, no. 5-6 (May 2009): 611–18. http://dx.doi.org/10.1177/096368970901805-617.
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The effect of adipose tissue-derived stem cells (ASCs) in combination with heparin transplantation on acute liver failure mice with carbon tetrachloride (CCl4) injection was investigated. CCl4 is a well-known hepatotoxin and induces hepatic necrosis. Heparin did not affect the viability of ASCs for at least 24 h. The injection of heparin into the caudal tail vein decreased slightly the activities of the alanine aminotransferase (ALT), asparate aminotransferase (AST), and lactate dehydrogenase (LDH) in plasma. In the transplantation of ASCs (1 × 106 cells) group, there was a trend toward decreased activities of all markers. However, four out of six mice died of the lung infarction. In the transplantation of ASCs in combination with heparin group, there was also a trend toward decreased activities of all markers. In addition, all mice survived for at least the duration of the study period. In conclusion, the transplantation of ASCs in combination with heparin was thus found to effectively treat acute liver failure.
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Tuan, Jessica, Jehanzeb Kayani, Ann Fisher, Brian Kotansky, Louise Dembry, Louise Dembry, and Rupak Datta. "613. Clinical Outcomes Following Dalbavancin Administration during Outpatient Parenteral Antimicrobial Therapy." Open Forum Infectious Diseases 8, Supplement_1 (November 1, 2021): S410. http://dx.doi.org/10.1093/ofid/ofab466.811.
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Abstract Background Dalbavancin, a lipoglycopeptide with prolonged half-life targeting Gram-positive organisms, is approved for treatment of acute bacterial skin and soft tissue infection. It reduces hospital duration in patients with barriers to short-term rehabilitation or outpatient parenteral antimicrobial therapy (OPAT). Increasing evidence supports the off-label use of dalbavancin to treat other types of infection. We conducted a quality improvement study to evaluate outcomes following dalbavancin administration. Methods We performed a cohort study of recipients of ≥1 dose of dalbavancin from 1/31/2016-1/31/2021 at the Veterans Affairs Connecticut Healthcare System. Demographic, comorbidity, microbiological, antibiotic duration prior to dalbavancin, indication for dalbavancin, and type of infection data were collected. Outcomes included 1) lab abnormalities: hepatotoxicity within 2 weeks of dalbavancin; 2) clinical cure: resolution of symptoms of infection within 90 days; 3) all-cause readmission within 90 days; and 4) all-cause mortality within 90 days. Results 42 patients met criteria. Median age was 69 years (range, 32-91), 100% were male, 55% (n=23) had diabetes, 31% (n=13) had liver disease, 36% (n=15) had other immunosuppressive conditions, and 12% (n=5) had substance use disorder (SUD). All received their first dose as inpatients. Median hospital duration was 8 days (range, 1-32). 4 (10%) required critical care. Median antibiotic duration prior to dalbavancin was 7 days (range, 1-42). Indications included ineligibility for OPAT (n=21, 50%), pharmacologic reasons (n=10, 24%), ineligibility for peripherally inserted central catheter (n=6, 14%), or SUD (n=5, 12%). Common microorganisms were Staphylococcus spp. (n=22, 52%), polymicrobial (n=13, 31%), and Corynebacterium spp. (n=10, 24%). 93% (n=39) had clinical cure of infection; readmissions and mortality were rare (Table 1). Conclusion Dalbavancin was associated with clinical cure for diverse infections with low rates of adverse events, readmission and mortality in patients ineligible for traditional OPAT. Although confirmatory data are needed from larger studies, dalbavancin appears to be a versatile therapeutic agent for Gram-positive infections. Disclosures All Authors: No reported disclosures
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Karlsen, Jon, and Johnny Nilsson. "Club Shaft Weight in Putting Accuracy and Perception of Swing Parameters in Golf Putting." Perceptual and Motor Skills 105, no. 1 (August 2007): 29–38. http://dx.doi.org/10.2466/pms.105.1.29-38.
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This study assessed how shaft weight influenced golf putting accuracy and subjective perception of swing parameters. Three putters of different shaft weight (100, 420, and 610 gm) were tested by 24 club players. Distance and deviation in direction were measured, and subjective ratings of the putters recorded. Subjects hit the ball further with lighter shafts. The mean distance hit was 100.2, 99.3, and 98.1% of the target distance for the normal, medium, and heavy putter shafts, respectively. Subjectively, the medium heavy putter was rated best on “overall feeling” and it was also rated better than the normal on “feeling of stability in the downswing.” The heaviest putter was rated as too heavy by 23 of 24 subjects. There were no significant differences between the putter clubs in distance and directional putting accuracy. The major findings are that the golfers putted 2.1% longer with the 100 gm shaft than with the 610 gm shaft and that the perception of overall feeling of the putter club was not related to performance.
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Skorokhod, A. A., A. S. Petrov, A. R. Kozak, M. A. Atyukov, A. O. Nefedov, and P. K. Yаblonskiy. "Efficiency and safety of video-assisted mediastinal lymphadenectomy in the treatment of non-small cell lung cancer." Grekov's Bulletin of Surgery 179, no. 6 (April 2, 2021): 24–33. http://dx.doi.org/10.24884/0042-4625-2020-179-6-24-33.
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INTRODUCTION. A number of studies demonstrate the advantage of bilateral mediastinal lymphadenectomy in surgery of non-small cell lung cancer (nSCLC). For surgical approach to the opposite mediastinum for many years there were proposed sternotomy, video-thoracoscopy, and transcervical video-assisted interventions. In our practice, we use videoassisted mediastinal lymphadenectomy (VAMLA).The OBJECTIVE was to learn the efficiency and safety of VAMLA in surgery of NSCLC.METHODS AND MATERIALS. The study included the materials of examination and treatment of 102 patients with NSCLC. 102 patients were divided into 2 groups. In the 1st group (54 patients), VAMLA and lung resection were performed. In the 2nd group (48 patients): anatomical lung resection and systematic ipsilateral lymphadenectomy (SLD) were performed.RESULTS. The average number of remote lymph node stations in group 1 was (7.8±1.7); in group 2 – (4.5±1.2) (p<0.05). The average number of lymph nodes was 26±8.6 compared to (14.3±6) in both groups, respectively (p<0.05). «Occult» pN2-N3 metastasis was detected in 20 % (7/34) of patients of the group 1 and 6.5 % (2/31) of patients of the group 2 (p<0.05). The level of postoperative complications in both groups was 33.4 vs. 29.2 %, respectively (p>0.05). The duration of the postoperative day ((12.7±4.9) vs. (13.7±6.5)) and the duration of pleural drainage ((5.5±4.2) vs. (5.8±4.4)) did not differ in both groups (p>0.05).CONCLUSION. VAMLA is an effective and safe method for evaluating the pN stage of NSCLC. Performing VAMLA in left-sided NSCLC allows removing significantly more lymph nodes and stations in comparison with SLD available in VATS and thoracotomy, which increases the accuracy of postoperative N-staging. The use of the VAMLA in minimally invasive surgery of right-sided NSCLC may be promising in cases of high risk of «occult» pN3 lesion, but requires further study of the role of contralateral lymphatic dissection.
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Swanepoel, Bianca, Aletta E. Schutte, Marike Cockeran, Krisela Steyn, and Edelweiss Wentzel-Viljoen. "Monitoring the South African population’s salt intake: spot urine v. 24 h urine." Public Health Nutrition 21, no. 03 (November 10, 2017): 480–88. http://dx.doi.org/10.1017/s1368980017002683.
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Abstract Objective The present study set out to determine whether morning spot urine samples can be used to monitor Na (and K) intake levels in South Africa, instead of the ‘gold standard’ 24 h urine sample. Design Participants collected one 24 h and one spot urine sample for Na and K analysis, after which estimations using three different formulas (Kawasaki, Tanaka and INTERSALT) were calculated. Setting Between 2013 and 2015, urine samples were collected from different population groups in South Africa. Subjects A total of 681 spot and 24 h urine samples were collected from white (n 259), black (n 315) and Indian (n 107) subgroups, mostly women. Results The Kawasaki and the Tanaka formulas showed significantly higher (P≤0·001) estimated Na values than the measured 24 h excretion in the whole population (5677·79 and 4235·05 v. 3279·19 mg/d). The INTERSALT formula did not differ from the measured 24 h excretion for the whole population. The Kawasaki formula seemed to overestimate Na excretion in all subgroups tested and also showed the highest degree of bias (−2242 mg/d, 95 % CI−10 659, 6175) compared with the INTERSALT formula, which had the lowest bias (161 mg/d, 95 % CI−4038, 4360). Conclusions Estimations of Na excretion by the three formulas should be used with caution when reporting on Na intake levels. More research is needed to validate and develop a specific formula for the South African context with its different population groups. The WHO’s recommendation of using 24 h urine collection until more studies are carried out is still supported.
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Bettin, Fernanda, Letícia O. da Rosa, Queli Montanari, Aldo J. P. Dillon, and Mauricio M. da Silveira. "Effect of pH and Temperature on the Growth and Laccases Production in the Cultivation of Pleurotus sajor-caju PS-2001 in Stirred-tank Bioreactor." Current Biotechnology 9, no. 3 (December 21, 2020): 219–29. http://dx.doi.org/10.2174/2211550109999200908090053.
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Background: Laccases are multi-copper enzymes that oxidize phenolic/aromatic compounds and represent a promising alternative to environmental decontamination processes and biotechnological applications. Objective: The effects of pH and temperature on the growth and the production of laccases during the cultivation of Pleurotus sajor-caju PS-2001 in stirred-tank bioreactor were assessed. Methods: Assays were performed at fixed pH values from 4.5 to 7.5 (28°C) and at temperatures from 24 to 36°C (pH 6.5). Results: In pH testing, larger biomass concentration (4.5 g L-1) was reached at pH 5.5, whereas concentrations of 3.7, 3.1 and 1.7 g L-1 were measured at pH 4.5, 6.5 and 7.5, respectively. With ABTS as substrate, peaks of laccases activity of 50, 30 and 24 U mL-1, at pH 6.5, 5.5 and 7.5, respectively, were detected. Under different temperatures, higher mycelial concentrations (3.0 g L-1) were quantified at 66 hours at 28°C, while concentrations below 2.0 g L-1 were observed at 24, 32, and 36°C. Maximum laccases activities of 50, 42, 6 and 5 U mL-1 were obtained at 28, 32, 24, and 36°C, respectively. In all tests, the presence of other phenol oxidases – total peroxidase, manganese peroxidase, lignin peroxidase and veratryl alcohol oxidase – was observed. Conclusion: The results indicate that variations in pH and temperature during fungal cultivation strongly affect the enzymatic activity and growth kinetics of P. sajor-caju PS-2001 in a stirredtank bioreactor.
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Anderson, Jeff. "611 High Temperature Acclimation and Catalase Stability in Pepper Leaves." HortScience 35, no. 3 (June 2000): 502D—502. http://dx.doi.org/10.21273/hortsci.35.3.502d.
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Activated forms of oxygen, including hydrogen peroxide, have been implicated in plant responses to stress. Environmental stresses may increase prooxidants, impair defense systems, or both. Stress acclimation may involve changes in capacity or stability of activated oxygen defenses. Catalases and peroxidases are the primary enzymatic detoxifiers of hydrogen peroxide in most plant tissues. Pepper leaf disks treated with hydrogen peroxide solutions from 0 to 100 mM showed increased electrolyte leakage and ethylene and methanol evolution with increasing concentration, but changes were slight compared to freeze-killed tissues. Data suggested that pepper leaves had considerable capacity to detoxify hydrogen peroxide. Cellular damage in heat-stressed tissues occurred over a similar temperature range that catalase activity declined. Leaf disks exposed to 24 to 59 °C for 15 min exhibited a sigmoidal electrolyte leakage response curve with an inflection at 51.5 °C. A similar plot of catalase activity vs. temperature exhibited an inflection point at 53.1 °C. Thermotolerance of plants exposed to the acclimating regime of 38 °C day/30 °C night increased from 50.7 to 53.9 °C based on electrolyte leakage. Catalase activity also showed an adaptive response with the inflection point increasing from 52.6 to 56.8 °C. It appears that catalase activity remains stable to a higher temperature in acclimated leaves, with similar activity in nonstressed control and acclimated plants.
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., Muhamad Ma'ruf, Dwi Putra Buana Sakti, and Handry Sudiartha Athar. "CUSTOMER EXPERIENCE SEBAGAI MEDIASI PENGARUH MULTI HALAL FRIENDLY-HOTEL ATRIBUTES TERHADAP KEPUASAN PELANGGAN GRAND MADANI HOTEL MATARAM." JMM UNRAM - MASTER OF MANAGEMENT JOURNAL 9, no. 4a (December 29, 2020): 27. http://dx.doi.org/10.29303/jmm.v9i4a.613.
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This study aims to analyze the effect of multi halal friendly hotel attributes on customer satisfaction at Grand Madani Hotel Mataram, West Nusa Tenggara Province, Indonesia using associative quantitative methods. In this study, the population is all hotel guests who have stayed at the Grand Madani Hotel Mataram. The number of samples used in this study were 105 samples using nonprobability sampling. The data used in this study are primary and secondary data. Data collection was done using a questionnaire / google form. Data analysis using path analysis using SEM AMOS Version 24 as a data processing tool. The results of this study indicate that: 1. Multi halal friendly hotel attributes have no significant effect on customer experience; 2. Customer experience has a significant effect on customer satisfaction 3. Multi halal friendly hotel attributes have no significant effect on customer satisfaction; 4. Multi halal friendly hotel attributes have a significant positive effect on customer satisfaction after going through the customer experience intervening variable.Keywords:Multi halal friendly hotel attributes; customer experience; customer satisfaction; sharia hotel
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HOSSAIN, MD ISMAIL, FATEMA HOQUE SHIKHA, and MAHMUDUL HASAN. "Post-mortem changes in three fresh water eels, Mastacembelus armatus, Macrognathus aculeatus and Mastacembelus pancalus during ice storage." Bangladesh Journal of Fisheries 32, no. 1 (July 4, 2020): 95–106. http://dx.doi.org/10.52168/bjf.2020.32.11.
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The progress of rigor-mortis and the post-mortem changes in three species of freshwater eelsduring storage at room temperature (30° to 35°C) and ice (0°C) were studied. Rigor-mortis in fish sample atroom temperature, started 1hr after spiking and it reached to a maximum of 76%, 57.14% and 100% within 4hrs after death in Mastacembelus armatus (Baim), Macrognathus aculeatus (Tara baim) and inMastacembelus pancalus (Guchi), respectively. In ice stored eels rigor also started within one hour of spikingand gradually reached to a maximum of 88.89% within 3 hrs in Baim and 85.71%, 100% in Tara baim and inGuchi respectively within 2 hrs. Rigor continued for 17, 14 and 18 hrs in Baim, Tara baim and Guchi,respectively and then started to relax. The pH of the muscle was 6.84, 6.80 and 6.92 immediately after catchin Baim, Tara baim and in Guchi, respectively which decreased gradually with the lapse of storage periodregardless of the storage temperature. The organoleptic quality of eels during ice storage was assessed on thebasis of sensory parameters. Baim was found in acceptable conditions for 24 days whereas Tara baim andGuchi were in acceptable condition for 28 days in ice storage. Percent moisture content increased but proteinand lipid contents decreased in almost all the fish samples with the lapse of storage period but a little changewas observed in ash content. The TVB-N, peroxide and NPN value remained within the recommended valueup to 24 days in Baim and 28 days in Tara baim and Guchi. The initial bacterial loads were 6.5×103,6.2×103 And 6.3×103 CFU/g in Baim, Tara baim and in Guchi, respectively. At the end of the 24 days ofice storage, bacterial load increased in all the fish samples.
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Angulo Lozano, J. C., L. F. Sánchez Musi, R. Racilla González, and J. F. Virgen Gutiérrez. "Penile Cancer in a tertiary care hospital in Mexico City: Risk factors description and frequencies in Mexican population." ANALES RANM 139, no. 139(02) (August 2022): 196–201. http://dx.doi.org/10.32440/ar.2022.139.02.org03.
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Objective: To determine and analyze the prevalence of the principal risk factors in patients with penile cancer in General Hospital “Dr. Eduardo Liceaga” and their survival rate at 24 months. Methods: Cross-sectional, descriptive study. The population (n=93) are hispanic, adult male residents of Mexico City with penile cancer diagnosed. The variables analyzed are: Age, smoking status, HPV status, presence of vascular invasion, treatment and survival status after 24 months. Results: Mean age (n=93) was 57.87. 55.9% were active smokers. 100% were uncircumcised. Only 17.2% were HPV +. Squamous Cell Carcinoma was the predominant type with 86%. 18.3% had vascular invasion. At 24 months after diagnosis with treatment 84.9% patients were alive, 6.5% died of cancer related causes, 6.5% of other causes and (2.2%) of chemotherapy toxicity. Conclusion: This infrequent disease requires a high quality multidisciplinary treatment. Squamous cell carcinomas are the predominant type in Mexico City, risk factors such as smoking, uncircumcised, age and HPV infection were present. HPV vaccines in men could help reduce a proportion of malignancies but there is no data related. Adjuvant therapy should be considered since the survival rate at 24 months of study was lower than first world countries. Periodic evaluation of chemotherapy adverse effects should be considered since a significant proportion died because of toxicity.
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Tseng, Li-Wei, Chih-Hsuan Chen, Yu-Chih Tzeng, Po-Yu Lee, Nian-Hu Lu, and Yury Chumlyakov. "Microstructure and Superelastic Properties of FeNiCoAlTi Single Crystals with the <100> Orientation under Tension." Crystals 12, no. 4 (April 14, 2022): 548. http://dx.doi.org/10.3390/cryst12040548.
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The microstructure and superelastic response of an Fe41Ni28Co17Al11.5Ti2.5 (at.%) single crystal along the <100> orientation was investigated under tension at room temperature after aging at 600 °C for 24 h. From the superelastic results, the samples aged at 600 °C for 24 h exhibited 4.5% recoverable strain at room temperature. The digital image correlation (DIC) method was used to observe the strain distribution during the 6.5% applied strain loading. The DIC results showed that the strain was uniformly distributed during the loading and unloading cycles. Only one martensite variant was observed from the DIC results. This was related to the aging heat treatment times. The martensite morphology became a single variant with a longer aging time. The thermo-magnetization results indicated that the phase transformation and temperature hysteresis was around 36 °C. Increasing the magnetic field from 0.05 to 7 Tesla, the transformation temperatures increased. The maximum magnetization was 160 emu/g under the magnetic field of 7 Tesla. From the transmission electron microscopy results, the L12 precipitates were around 10 nm in size, and they were high in Ni content and low in Fe content.
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Gorbunov, N. A., A. P. Dergilev, L. D. Sidorova, and V. I. Kochura. "DIAGNOSTIC CAPABILITIES OF DIGITAL FLUOROGRAPHY IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE." Bulletin of Siberian Medicine 14, no. 1 (February 28, 2015): 24–31. http://dx.doi.org/10.20538/1682-0363-2015-1-24-31.
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The purpose of the study – to examine the capabilities of the method of digital chest fluorography in diagnosis of chronic obstructive pulmonary disease (COPD) of different stages. By chest digital fluorography 247 patients with clinically diagnosed COPD stage I–III were examined. The average age of the patients was (55.3 1.7) years (16 to 88 years), forced expiration volume for the first second (FEV1) was (59.17 16.28) %. It was shown that for patients with COPD stage I most characteristic radiographic symptom was emphysema of the lung in the upper regions (26 (48%) of 54 patients); the mean values of the inspiratory lung optical density (ILOD) ranged from (703.48 2.15) to (807.47 5.61) optical density units (ODU), the expiratory lung optical density (ELOD) – from (786.05 6.15) to (830.23 4.71) ODU. For patients with COPD stage II the most common radiographic sign was the presence of amplification and deformation pulmonary pattern (95 (89%) of 107 patients); the mean values of ILOD ranged from (646.20 4.94) to (791.38 2.81) ODU, ELOD – from (677.34 9.91) to (813.91 3.29) ODU. Patients with COPD stage III were characterized by a combination of amplification and deformation pulmonary pattern (77 (90%) of 86 patients) with lung emphysema (51 (59%) of 86 patients); the mean values of the inspiratory lung optical density ranged from (765.93 16.4) to (863.37 15.83) ODU, expiratory lung optical density – from (826.80 11.64) to (881.37 4.29) ODU. Thus, we concluded that the method of digital chest fluorography, possessing low dose burden to the patient, can detect the characteristic radiological symptoms and determine the stage of the disease up to the values of the lung optical density in patients with COPD.
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Akiyama, Shintaro, Jacob Ollech, Victoria Rai, Laura Glick, Jorie Singer, Jordan Karpin, Tina Rodriguez, et al. "24 ENDOSCOPIC AND CLINICAL CHARACTERISTICS OF POUCHITIS INVOLVING THE RECTAL CUFF IN PATIENTS WITH INFLAMMATORY BOWEL DISEASE TREATED BY PROCTOCOLECTOMY WITH ILEAL POUCH-ANAL ANASTOMOSIS." Inflammatory Bowel Diseases 26, Supplement_1 (January 2020): S13—S14. http://dx.doi.org/10.1093/ibd/zaa010.031.
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Abstract Background For patients with inflammatory bowel disease (IBD), surgical intervention is sometimes required due to medically refractory colitis or development of neoplasia. Restorative proctocolectomy with ileal pouch-anal anastomosis (IPAA) is the most common procedure for patients with colitis. However, pouchitis can develop in up to 80% of patients after the surgery and significantly impairs quality of life. Despite the high prevalence of pouchitis, endoscopic phenotypes have not been clarified. This study assessed the frequency and characteristics of inflammation involving the rectal cuff. Methods This is a retrospective study from a tertiary IBD center of IBD patients treated by total proctocolectomy with IPAA and subsequently underwent pouchoscopies at the University of Chicago between January 2007 and September 2019. We reviewed the endoscopic findings in different areas of the pouch: the pre-pouch ileum, inlet, “tip of the J”, proximal and distal pouch, anastomosis, rectal cuff, anal canal, and perianal area. This analysis evaluated all available pouchoscopies per patient and included patients with normal cuff and those with cuffitis noted in every pouchoscopy. Demographic and endoscopic data were assessed to compare patients with normal cuff and those with cuffitis. Fisher’s test was used for a univariate analysis to assess factors contributing to cuffitis. Logistic regression analysis was performed as a multivariate analysis including univariate variables with a P-value < 0.15. Results We reviewed 1,081 pouchoscopies from 426 IBD patients who underwent proctocolectomy with IPAA and identified 184 patients (43%) with normal cuff and 107 (25%) with cuffitis. Of these 291 patients, 57% were men, 92% were Caucasian, the mean age at the diagnosis (SD) was 26 ± 12 years, and mean BMI 26 ± 5 kg/m2. The diagnosis before surgery for these patients were for ulcerative colitis (91%), indeterminate colitis (5.8%), and Crohn’s disease (1.7%). A significant difference (P = 0.003) was found in the frequency of pouchitis between patients with normal cuff (126/184, 69%) and those with cuffitis (90/107, 84%) (Table 1). Although not statistically significant, the frequency of pouch failure in patients with cuffitis (13/107, 12%) was higher than those with normal cuff (12/184, 6.5%). Multivariate analysis showed pouchitis was significantly associated with cuffitis (OR = 2.2; 95% CI = 1.2–4.2; P = 0.01) (Table 2). Endoscopic data showed that the pre-pouch ileum was significantly (P = 0.001) involved in patients with cuffitis (45/90, 50%) compared with those with normal cuff (36/126, 29%). Conclusion Our analysis of 291 patients suggested that cuff inflammation can be a significant risk factor of pouchitis and is significantly associated with the development of inflammation in the pre-pouch ileum. Cuffitis can be a therapeutic target to improve J pouch outcomes.
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Miarka, Bianca, Ciro José Brito, John Amtmann, Cláudio Córdova, Fabio dal Bello, and Suzi Camey. "Suggestions for Judo Training with Pacing Strategy and Decision Making by Judo Championship Phases." Journal of Human Kinetics 64, no. 1 (October 15, 2018): 219–32. http://dx.doi.org/10.1515/hukin-2017-0196.
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Abstract The present study aimed to compare pacing and decision making of athletes competing in judo, with particular attention paid to effort-pause ratios occurring in the championship phases of the Olympic Games and non-Olympic Games. The sample was composed of 53,403 sequential actions analyzed during 611 performances of the non-Olympic Games (eliminatory n = 330, quarterfinals n = 60, semi-final n = 88, repechage n = 21, third place playoff n = 26, and final n = 79) and 163 from the Olympic Games (eliminatory n = 71, quarterfinals n = 13, semi-final n = 26, repechage n = 20, third place playoff n = 24, and final n = 14). The analysis of effort-pause ratios included separating bouts into states of approach, gripping, attack, groundwork and pause, according to frequency and time. A Markov multi-state model and analysis of variance were applied (p ≤ 0.05). Approach time presented differences of the eliminatory Olympic Games (7.3 ± 3.2 s) versus final non-Olympic Games (6.0 ± 2.2s), and the third place playoff Olympic Games (8.1 ± 2.3 s) versus semi-final (6.2 ± 2.4 s) and third place playoff (5.9 ± 2.1 s) of the non-Olympic Games, and the semi-final Olympic Games (8.6 ± 2.3 s) versus eliminatory (6.5 ± 2.3 s), quarter-finals (6.5 ± 1.7 s), semi-final (6.2 ± 2.4 s), repechage (6.2 ± 2.2 s), third place playoff (5.9 ± 2.1 s), and final (6.0 ± 2.0 s) of the non-Olympic Games. Pause time presented differences of the semi-final Olympic Games (6.8 ± 2.1 s) versus eliminatory (5.1 ± 3.1 s). The present data suggest a focus on pacing strategy during championship phases, which mimic the requirements of judo combats.
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Loewen, G. M., B. A. Holm, L. Milanowski, L. M. Wild, R. H. Notter, and S. Matalon. "Alveolar hyperoxic injury in rabbits receiving exogenous surfactant." Journal of Applied Physiology 66, no. 3 (March 1, 1989): 1087–92. http://dx.doi.org/10.1152/jappl.1989.66.3.1087.
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We have previously demonstrated that instillation of a calf lung surfactant extract (CLSE) in rabbits after exposure to 100% O2 for 64 h mitigates the progression of lung pathology after return to room air (J. Appl. Physiol. 62: 756–761, 1987). In the present study, we investigated whether we could prevent or reduce the onset and development of hyperoxic lung injury by sequential instillations of CLSE during the hyperoxic exposure. Rabbits were exposed to 100% O2. CLSE (125 mg, approximately 170 mumol of phospholipid) was suspended in 10 ml of sterile saline and instilled intratracheally into their lungs, starting at 24 h in O2, a time at which no physiological or biochemical injury was detected, and at 24-h intervals thereafter. Control rabbits breathed 100% O2 and received either equal volumes of saline or no instillations at all. CLSE-instilled rabbits had higher arterial PO2 (Pao2) values throughout the exposure period and survived longer when compared with saline controls [120 +/- 4 vs. 102 +/- 4 (SE) h; n greater than or equal to 10; P less than 0.05]. At 72 h in O2, CLSE-instilled rabbits had significantly higher lavageable alveolar phospholipid levels (12.5 +/- 1.5 vs. 5 +/- 1 mumol/kg) and total lung capacities (41 +/- 2 vs. 25 +/- 3.5 ml/kg) and lower levels of alveolar protein (24 +/- 3 vs. 52 +/- 8 mg/kg), minimum surface tension (2 +/- 1 vs. 26.1 dyn/cm), and lung wet-to-dry weights (5.9 +/- 0.2 vs. 6.5 +/- 0.3). After 72 h in O2, lungs from both CLSE- and saline-instilled rabbits showed evidence of diffuse hyperoxic injury. However, atelectasis was less prominent in the former. We concluded that instillation of CLSE limits the onset and development of hyperoxic lung injury to the alveolar epithelium of rabbits.
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Liu, Gexiu, Chengcheng Liu, Aizhen Zhu, Jing Lai, Xiaoyu Chen, and Dongmei He. "Estradiol Promotes Hematopoietic Recovery of HSCT in Mice by Improving Bone Marrow Microenvironment." Blood 120, no. 21 (November 16, 2012): 4662. http://dx.doi.org/10.1182/blood.v120.21.4662.4662.
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Abstract Abstract 4662 INTRODUCTION: Although hematopoietic stem cell transplantation (HSCT) has made great progress in recent years, the transplant success rate needs to be further improved. Due to the poorly hematopoietic recovery, part of the patients who received donated bone marrow can not go out of danger safely. A small mount of cord blood for adult's hematopoietic reconstitution is often not enough. Therefore, to further enhance the donor HSC engraftment and improve hematopoietic recovery is crucial. Studies have been reported that the hematopoietic microenvironment including mesenchymal stem cells(MSCs), endothelial cells, osteoblasts, and so on has been injured during conditioning regimens by irradiation or drugs. Recent reports showed that estradiol not only induced MSCs into osteoblasts, but also inhibited differentiation of them into adipocytes, promoted their proliferation, protected them from hydrogen peroxide-induced apoptosis, and enhanced the recruitment of endothelial progenitor cells. Accordingly, we observed the effects of estradiol on hematopoietic recovery after HSCT in mice. METHODS: 180 female BALB/c mice(7–9 weeks old) were separated randomly into control group(I), TBI group(II, myeloablative total body irradiation of 8.5 Gy 60Co γ ray), TBI plus HSCT group(III, irradiation of 8.5 Gy 60Co γ ray, transplanted with 5.0×105 Lin#x2610;−, Sca-1+, c-kit#x2610;+ cells separated from bone marrow by MACS kit per mouse), and TBI plus HSCT and estradiol group(IV, irradiation of 8.5 Gy 60Co γ ray, administrated estradiol using slow-release pellets(100ng/d for 1 month), transplanted with 5.0×105 Lin#x2610;−, Sca-1+, c-kit#x2610;+ cells each mouse), 40 mice for each group, the rest were used to separate HSCs. Mice were subjected to irradiation of 8.5 Gy at rate of 0.65Gy/min with a distance of 2.43 meters from the source. The general condition of all mice was continuously observed: like mental state, vitality, coat color, body weight, diet, etc. Peripheral blood white blood cells(WBC) were counted, and pathological changes of bone marrow were evaluated by hematoxylin and eosin stain under microscope at Day 3, 6, 12, 18, 24, and 30 after administration, analyzing 8 mice of each group at every time point. The basement membranes were observed by the transmission electron microscope (TEM). PAS-8000 pathological image analysis system was used to measure the percentage of intramedullary fat cells area in sections. RESULTS: General conditions of control group mice were good during observation, and there were few adipocytes in bone marrow. Peripheral blood WBC count of control group mice was stable, which was (10.8±1.7)×109 cells/L on the 3rd day, significantly different from that of other groups mice. General conditions of experimental groups mice were worse, especially group II, of which the mice died of hematopoietic disorders during Day 8 –14. WBC count of three experimental groups dropped rapidly, while the amount of adipocytes in bone marrow increased significantly on the 6th day, reaching a peak on the 24th day. Percents of fat vacuoles area in bone marrow of group III mice were (19.2±3.0)%, (64.6±5.1)%, (85.6±6.1)%, (91.7±7.3)%, and (84.2±6.5)% at Day 6, 12, 18, 24, and 30, respectively, and corresponding WBC count were (5.3±1.1)×105, (8.6±1.2)×106, (6.7±1.1)×107, and (7.8±1.1)×109, (9.7±1.8)×109 cells/L, respectively. But group IV had less adipocytes than other two experimental groups at corresponding time points. Percents of fat vacuoles area in group IV mice were (10.7±2.9)%, (33.4±4.9)%, (55.6±6.1)%, (81.5±6.8)%,and (69.2±6.7)%, respectively, and corresponding WBC count were (6.8±1.4)×105, (1.6±0.4)×107, (2.6±0.7)×108, and (8.9±1.1)×109, (10.1±2.1)×109 cells/L, respectively. Moreover, there was less intramedullary hemorrhage in bone marrow of the group IV mice than in group II and III at Day 3. Basement membranes were more completed showed by TEM. CONCLUSION: Estradiol promoted hematopoietic recovery of HSCT in mice, which might through improving bone marrow microenvironment. Disclosures: Liu: National Natural Science Fundation of China: Research Funding. Liu:National Natural Science Foundation of China: Research Funding.
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PRASKURNICHIY, E. A., A. N. KNYAZEV, and I. I. BEGUNOVA. "Comparative assessment of the possibilities of multispiral computer tomography and ultrasound dopplerography in the verification of atherosclerotic lesions of major vessels." Practical medicine 18, no. 5 (2020): 105–9. http://dx.doi.org/10.32000/2072-1757-2020-5-105-109.
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The purpose — to compare the diagnostic potential of multispiral computer tomography and ultrasound dopplerography in the assessment of atherosclerotic lesions of the cerebral arteries in metabolic syndrome. Material and methods. The study included 78 patients. The group consisted of 44 men (56,4%) and 34 women (43,6%); the average age was 62 ± 1,2 years. Coronary heart disease was detected in 54 (69,2%), and no signs were registered in 24 (30,8%). Anamnestically, 19 (24,4%) people were diagnosed with a stroke, and 18 (23%) people had a myocardial infarction. 42 people (53,8%) had verified dyscirculatory encephalopathy of various degrees. 34 (43,6%) people suffered from hypertension. 42 people had disorders of carbohydrate metabolism: 12 (15,3%) — type 2 diabetes, 30 (38,4%) — metabolic syndrome. The patients were divided into 3 groups: 1) persons without metabolic syndrome — 48 people (61.5% of the total number of examined); 2) persons with metabolic syndrome without type 2 diabetes — 18 people (23,1% of the total number of examined); 3) persons with type 2 diabetes — 12 people (15,4% of the total number of examined). Results. Atherosclerotic plaques in the carotid arteries were detected in 78 people by multispiral computer tomography and in 51 patients by ultrasound dopplerography of these vessels. Conclusions. In general, the use of multispiral computer tomography to detect atherosclerotic lesions of the vascular bed is the most preferable in comparison with ultrasound dopplerography, especially in patients with a high risk of cardiovascular events. While for patients who do not belong to this group, ultrasound dopplerography (a simple, accessible, informative technique) can be used as the primary screening.