Dissertations / Theses on the topic '3D pathology'
To see the other types of publications on this topic, follow the link: 3D pathology.
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 19 dissertations / theses for your research on the topic '3D pathology.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Al-Kwifi, Osama. "Development of 3D magnetic resonance angiography for the detection of vascular pathology." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ60407.pdf.
Full text
2
Meija, Jorge, Richard Van Hoesel, Anna O'Brien, Ingrid McClelland Yeend, Cong-Van M. Nguyen, Earl E. Johnson, and Harvey Dillon. "Acoustic Simulation Using 3d Modeling of the Development of a Super-directional System, past, Present and Future." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etsu-works/1759.
Full textAbstract:
It is well established that modern directional microphones in hearing aids provide listeners with an improved speech understanding in noise. De-spite this, the impact of directional microphones in real life conditions is limited. In fact, hearing in noise remains one of the biggest problems for hearing aid users. Fortunately, recent developments in super-directional technology, at least in laboratory settings, promise to deliver significant benefits to hearing aid users. Experiments suggest large improvements in speech understanding in noise and significant preference for highly directional systems. This advantage is often extrapolated to suggest equal advantages in real world listening situations. However, it is increasingly apparent that hearing in noise entails various complex tasks for the listener. Consequently, super-directional technology may be advantageous in some situations but may also have some limitations in its usage. Here we present a discussion of super-directional microphone technology based on several studies. In our research we have examined various factors that influence benefit such as beam width design, adaptation speed, preservation of spatial cues, vent sizes, acoustic scene, and reverberation. Our evidence appears to be confounded by individual characteristics of the listener such as age, hearing loss, personality traits, and cognition. All these factors combined will guide our discussions and thoughts about future research and development of super-directional systems
3
Ogunleke, Abiodun. "Imagerie chimique 3D de tumeurs du cerveau." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0040/document.
Full textAbstract:
L'histologie tridimensionnelle (3D) est un nouvel outil avancé de cancérologie. L'ensemble du profil chimique et des caractéristiques physiologiques d'un tissu est essentiel pour comprendre la logique du développement d'une pathologie. Cependant, il n'existe aucune technique analytique, in vivo ou histologique, capable de découvrir de telles caractéristiques anormales et de fournir une distribution3D à une résolution microscopique. Nous présentons ici une méthode unique de microscopie infrarouge (IR) à haut débit combinant une correction d'image automatisée et une analyse ultérieure des données spectrales pour la reconstruction d'image 3D-IR. Nous avons effectué l'analyse spectrale d'un organe complet pour un petit modèle animal, un cerveau de souris avec une tumeur de gliome implantée. L'image 3D-IR est reconstruite à partir de 370 coupes de tissus consécutives et corrigée à l'aide du tomogramme à rayons X de l'organe pour une analyse quantitative précise du contenu chimique. Une matrice 3D de spectres IR 89 x 106 est générée, ce qui nous permet de séparer la masse tumorale des tissus cérébraux sains en fonction de divers paramètres anatomiques,chimiques et métaboliques. Nous démontrons pour la première fois que des paramètres métaboliques quantitatifs (glucose, glycogène et lactate) peuvent être extraits et reconstruits en 3D à partir des spectres IR pour la caractérisation du métabolisme cérébral / tumoral (évaluation de l'effet de Warburg dans les tumeurs). Notre méthode peut être davantage exploitée en recherchant l'ensemble du profil spectral, en distinguant différents points de repère anatomiques dans le cerveau.Nous le démontrons par la reconstruction du corps calleux et de la région des noyaux gris centraux du cerveauThree-dimensional (3D) histology is a new advanced tool for cancerology. The whole chemical profile and physiological characteristics of a tissue is essential to understand the rationale of pathology development. However, there is no analytical technique, in vivo or histological, that is able to discover such abnormal features and provide a 3D distribution at microscopic resolution.Here, we introduce a unique high- throughput infrared (IR) microscopy method that combines automated image correction and subsequent spectral data analysis for 3D-IR image reconstruction. I performed spectral analysis of a complete organ for a small animal model, a mouse brain with animplanted glioma tumor. The 3D-IR image is reconstructed from 370 consecutive tissue sectionsand corrected using the X-ray tomogram of the organ for an accurate quantitative analysis of thechemical content. A 3D matrix of 89 x 106 IR spectra is generated, allowing us to separate the tumor mass from healthy brain tissues based on various anatomical, chemical, and metabolic parameters. I demonstrate for the first time that quantitative metabolic parameters (glucose, glycogen and lactate) can be extracted and reconstructed in 3D from the IR spectra for the characterization of the brain vs. tumor metabolism (assessing the Warburg effect in tumors). Our method can be further exploited by searching for the whole spectral profile, discriminating different anatomical landmarks in the brain. I demonstrate this by the reconstruction of the corpus callosum and basal ganglia region of the brain
4
Swenson, Robert David. "The effects of bioprinting materials on HEPM cell proliferation and cytokine release." Thesis, University of Iowa, 2018. https://ir.uiowa.edu/etd/6299.
Full textAbstract:
Objectives: Three-dimensional (3D) bioprinting is a manufacturing process that incorporates viable cells into a 3D matrix by adding layer upon layer of material. The objectives of this study are to characterize a novel matrix of collagen and hydroxyapatite and to assess the effects of the 3D bioprinting process on cytotoxicity, proliferation rate, and cytokine expression of Homo sapiens palatal mesenchyme (HEPM) cells.
Methods: For this, we prepared a 3D matrix of collagen and hydroxyapatite without and with cells. We used light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) to characterize the structure and arrangement of the collagen fibers. We then incubated the matrix with known standards of cytokines to measure adsorption. We assessed the proliferation and viability of HEPM cells in the presence of the 3D construct and after 3D bioprinting. Finally, we assessed the cytotoxicity of this matrix for HEPM cells and assessed its effect on the production of chemokines and cytokines. A one-way fixed effect ANOVA was fit to concentrations of cytokines and pairwise group comparisons were conducted using Tukey’s Honest Significant Differences test (p< 0.05).
Results: The matrix was found to contain interwoven strands of collagen and some hydroxyapatite crystals that did not absorb cytokines except for MIP-1a (p< 0.05). The matrix was found to be non-cytotoxic using an AlamarBlue® assay. We found that the cell proliferation rate was lower when seeded on the 3D construct than in 2D culture. We also found that the proliferation rate was very low for the HEPM cells in the 3D bioprinted constructs. In the presence of the 3D construct, the HEPM cells had similar expression profiles of the cytokines measured (P > 0.05 for GM-CSF, IL-6, IL-8, and RANTES).
Conclusion: 3D-bioprinting has the potential to be used in dentistry as a novel osteogenic bone grafting material. Here we show that a novel matrix of collagen and hydroxyapatite is non-cytotoxic to HEPM cells and does not induce a proinflammatory response.
5
Vijayan, Suvendra. "Reliability of 3D-printed mandibles constructed from CBCT volumes of different voxel sizes." Thesis, University of Iowa, 2018. https://ir.uiowa.edu/etd/6323.
Full textAbstract:
Objectives: The aim of the current study is to establish the reliability of linear cephalometric measurements made on mandibles and their respective 3D printed models created from different voxel resolutions from a cone beam CT machine.
Materials and methods: Ten dry mandibles obtained from the Department of Oral Pathology, Radiology and Medicine at The University of Iowa College Of Dentistry were used for this study. All mandibles were scanned on the i-CAT FLX cone beam CT machine (Imaging Sciences International, LLC, Pennsylvania, USA) using voxel resolutions of .30mm, .25mm and .20 mm in a 16cm x 8cm field of view using 360° rotation. The 3D models were reconstructed and saved as .STL files using 3D Slicer software and send to a 3D printer for printing. Two observers measured the 10 mandibles and 30 3D printed models. The measurement were repeated on 50% of the samples after at least one week interval. Cronbach’s alpha and intraclass correlation coefficient were calculated to measure reliability.
Results: Good to excellent interobserver and intraobserver reliability was achieved across most of the measurements. There was no difference in reliability across models made from different voxel sizes.
Conclusion: The current study successfully showed that the reliability of measurements made on 3D printed models of dry skull mandibles created using fused deposition modeling technique using images of different voxel sizes from an i-CAT FLX CBCT machine are valid, reproducible, and reliable and can be used for diagnostic and clinical purposes.
6
Erdogmus, Nesli. "Utilisation des données 3D pour la reconnaissance du visage." Phd thesis, Télécom ParisTech, 2012. http://pastel.archives-ouvertes.fr/pastel-00982374.
Full textAbstract:
Avec l'augmentation des applications de sécurité, un sujet particulier a attiré plus d'attention que de nombreux autres domaines en reconnaissance de formes : la biométrie - la reconnaissance des personnes en fonction de leur physique et/ou de leurs traits comportementaux. Parmi ces traits, le visage se distingue par un compromis favorable entre accessibilité et fiabilité. Dans cette thèse, nous avons principalement travaillé sur une modalité relativement plus récente en reconnaissance du visage : la forme 3D. Plus avantageuse que son homologue en 2D, car intrinsèquement invariant aux changements d'illumination de pose, l'analyse 3D du visage se heurte encore à des défis majeurs tels que les déformations de surface du visage dues aux expressions ou occultations. En conséquence, une étude approfondie est effectuée sur ce sujet incluant le prétraitement des données 3D du visage, l'annotation automatique, la simulation d'altération et d'expressions en 3D, l'extraction de caractéristiques 3D et l'analyse locale de surface.
7
Namati, Eman, and eman@namati com. "Pre-Clinical Multi-Modal Imaging for Assessment of Pulmonary Structure, Function and Pathology." Flinders University. Computer Science, Engineering and Mathematics, 2008. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20081013.044657.
Full textAbstract:
In this thesis, we describe several imaging techniques specifically designed and developed for the assessment of pulmonary structure, function and pathology. We then describe the application of this technology within appropriate biological systems, including the identification, tracking and assessment of lung tumors in a mouse model of lung cancer.
The design and development of a Large Image Microscope Array (LIMA), an integrated whole organ serial sectioning and imaging system, is described with emphasis on whole lung tissue. This system provides a means for acquiring 3D pathology of fixed whole lung specimens with no infiltrative embedment medium using a purpose-built vibratome and imaging system. This system enables spatial correspondence between histology and non-invasive imaging modalities such as Computed Tomography (CT), Magnetic Resonance Imaging (MRI) and Positron Emission Tomography (PET), providing precise correlation of the underlying 'ground truth' pathology back to the in vivo imaging data. The LIMA system is evaluated using fixed lung specimens from sheep and mice, resulting in large, high-quality pathology datasets that are accurately registered to their respective CT and H&E histology.
The implementation of an in vivo micro-CT imaging system in the context of pulmonary imaging is described. Several techniques are initially developed to reduce artifacts commonly associated with commercial micro-CT systems, including geometric gantry calibration, ring artifact reduction and beam hardening correction. A computer controlled Intermittent Iso-pressure Breath Hold (IIBH) ventilation system is then developed for reduction of respiratory motion artifacts in live, breathing mice. A study validating the repeatability of extracting valuable pulmonary metrics using this technique against standard respiratory gating techniques is then presented.
The development of an ex vivo laser scanning confocal microscopy (LSCM) and an in vivo catheter based confocal microscopy (CBCM) pulmonary imaging technique is described. Direct high-resolution imaging of sub-pleural alveoli is presented and an alveolar mechanic study is undertaken. Through direct quantitative assessment of alveoli during inflation and deflation, recruitment and de-recruitment of alveoli is quantitatively measured. Based on the empirical data obtained in this study, a new theory on alveolar mechanics is proposed.
Finally, a longitudinal mouse lung cancer study utilizing the imaging techniques described and developed throughout this thesis is presented. Lung tumors are identified, tracked and analyzed over a 6-month period using a combination of micro-CT, micro-PET, micro-MRI, LSCM, CBCM, LIMA and H&E histology imaging. The growth rate of individual tumors is measured using the micro-CT data and traced back to the histology using the LIMA system. A significant difference in tumor growth rates within mice is observed, including slow growing, regressive, disappearing and aggressive tumors, while no difference between the phenotype of tumors was found from the H&E histology. Micro-PET and micro-MRI imaging was conducted at the 6-month time point and revealed the limitation of these systems for detection of small lesions ( < 2mm) in this mouse model of lung cancer. The CBCM imaging provided the first high-resolution live pathology of this mouse model of lung cancer and revealed distinct differences between normal, suspicious and tumor regions. In addition, a difference was found between control A/J mice parenchyma and Urethane A/J mice normal parenchyma, suggesting a 'field effect' as a result of the Urethane administration and/or tumor burden. In conclusion, a comprehensive murine lung cancer imaging study was undertaken, and new information regarding the progression of tumors over time has been revealed.
8
Sweet, David Ryan. "Kruppel-Like Factor 2 Regulation of Aging and Healthspan: A Link Between the 3D Nucleome and Disease." Case Western Reserve University School of Graduate Studies / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1619459260412975.
Full text
9
Michel, Fabrice. "Multi-Modal Similarity Learning for 3D Deformable Registration of Medical Images." Phd thesis, Ecole Centrale Paris, 2013. http://tel.archives-ouvertes.fr/tel-01005141.
Full textAbstract:
Even though the prospect of fusing images issued by different medical imagery systems is highly contemplated, the practical instantiation of it is subject to a theoretical hurdle: the definition of a similarity between images. Efforts in this field have proved successful for select pairs of images; however defining a suitable similarity between images regardless of their origin is one of the biggest challenges in deformable registration. In this thesis, we chose to develop generic approaches that allow the comparison of any two given modality. The recent advances in Machine Learning permitted us to provide innovative solutions to this very challenging problem. To tackle the problem of comparing incommensurable data we chose to view it as a data embedding problem where one embeds all the data in a common space in which comparison is possible. To this end, we explored the projection of one image space onto the image space of the other as well as the projection of both image spaces onto a common image space in which the comparison calculations are conducted. This was done by the study of the correspondences between image features in a pre-aligned dataset. In the pursuit of these goals, new methods for image regression as well as multi-modal metric learning methods were developed. The resulting learned similarities are then incorporated into a discrete optimization framework that mitigates the need for a differentiable criterion. Lastly we investigate on a new method that discards the constraint of a database of images that are pre-aligned, only requiring data annotated (segmented) by a physician. Experiments are conducted on two challenging medical images data-sets (Pre-Aligned MRI images and PET/CT images) to justify the benefits of our approach.
10
Jensen, Lauren Elizabeth. "Mechanical preparation of oval-shaped root canals in mandibular premolars with the TRUShape 3D Conforming File: a micro-computed tomography study." Thesis, University of Iowa, 2017. https://ir.uiowa.edu/etd/5523.
Full textAbstract:
The TRUShape 3D Conforming File (TRUShape), a novel, S-shaped nickel-titanium (NiTi) rotary file, was developed to facilitate cleaning and shaping of irregular-shaped root canals. The purpose of this study was to evaluate the shaping ability of TRUShape compared to Vortex Blue (VB) when used in non-round, oval-shaped root canals by micro-computed tomography (MCT).
Thirty single-rooted human mandibular premolar teeth with radiographically similar root canal size and curvature were randomly allocated to two groups (N=15), and mechanically prepared with TRUShape or VB. Each tooth was submitted to MCT at 20 μm resolution at three time intervals: before shaping, and after shaping to an intermediate apical size 30 and a final apical size 40. Three-dimensional data sets were superimposed and evaluated for root canal volume, surface area, and treated surface. Matched axial slices in the apical, middle, and coronal root thirds were evaluated for area, roundness, and canal transportation expressed as center of mass shift (CMS). Data were statistically analyzed using parametric and non-parametric tests.
Root canal volumes increased similarly and significantly overall (p< 0.001; from an initial volume of 7.3±3.5 mm3 to an intermediate volume of 8.7±3.1 mm3 and a final volume of 9.9±3.0 mm3). Treated canal surface was significantly larger in the TRUShape group at both apical sizes 30 and 40 with 72±15% vs. 55±23% and 85±12% vs. 71±20% non-static voxels for TRUShape and VB, respectively (p< 0.05). Canal transportation was less than 100 μm in all but 8 out of 90 cross sections and was not significantly different between groups.
This MCT study demonstrated the TRUShape 3D Conforming File to be effective in the mechanical preparation, specifically, the surface treatment, of single-rooted premolars with non-round, oval-shaped root canals.
Funding was provided by the American Association of Endodontists (AAE) Foundation and the Department of Endodontics at the University of Iowa, College of Dentistry.
11
Massot, Corentin. "Texture et Perception 3D dans les Scènes Naturelles : Modèles d'Inspiration Biologique et Expérimentations Psychophysiques." Phd thesis, Université Joseph Fourier (Grenoble), 2006. http://tel.archives-ouvertes.fr/tel-00207512.
Full textAbstract:
Dans ce travail nous nous intéressons à l'analyse et l'extraction de l'information 3D (orientation et forme) contenue dans les images de scènes naturelles et des textures homogènes. Pour cela nous adoptons une approche pluridisciplinaire de la modélisation du système visuel.Nous présentons d'abord des expérimentations psychophysiques où nous avons cherché à évaluer la contribution relative des indices de variation de fréquence et de perspective linéaire pour la perception 3D.
Pour cela nous avons créé des stimuli spécifiques représentant des textures homogènes composées de masques de Gabor disposés sur une surface plane.
Le plan est vu en projection perspective suivant une inclinaison (slant) et une orientation (tilt) particulière.
La fréquence et l'orientation de chaque masque de Gabor sont déterminées en fonction du gradient de fréquence local et de la perspective linéaire locale définis par la projection.
Nous synthétisons ainsi des textures présentant uniquement une variation de fréquence ou une variation d'orientation ou les deux types de variation (en combinaison ou en conflit).
Pour chaque texture, une tâche de discrimination du slant et du tilt est effectuée. L'indice de variation de fréquence apparaît prépondérant dans l'estimation de l'inclinaison d'une surface par rapport à la perspective linéaire.
Par contre les deux indices jouent un role dans l'estimation de l'orientation.
Ces résultats valident l'utilisation de nos stimuli pour la perception 3D et permettent de préciser la décomposition de l'indice de texture en composantes élémentaires.
Basé sur cette approche, nous présentons un modèle biologiquement plausible d'analyse de la variation de fréquence au niveau de V1.
Nous modélisons la réponse des cellules complexes par des filtres log-normaux à variables séparables présentant différents avantages théoriques et pratiques par rapport aux filtres de Gabor classiquement utilisés.i
L'algorithme se compose d'une étape de prétraitement composé d'un filtrage rétinien pour ne conserver que les informations de texture et d'une décomposition de l'image en un ensemble d'imagettes similairement aux champs récepteurs des cellules corticales.
Une technique robuste d'estimation de la fréquence moyenne locale, indépendante de l'information d'orientation et correspondant à une combinaison simple de lensemble des filtres est appliquée à chaque imagette.
La mesure de la variation locale de fréquence entre chaque imagette permet d'estimer le tilt et le slant de la surface étudiée ainsi que sa forme.
La méthode est évaluée sur différentes bases d'images et de textures. Elle s'avère comparable en précision aux autres techniques et s'applique à des textures irrégulières avec une moindre complexité calculatoire.
12
Mauconduit, Franck. "Imagerie cérébrale par résonance magnétique du tenseur de diffusion : de la modélisation à l'imagerie 3D haute résolution. Applications et "fibre tracking" dans un modèle de schizophrénie chez la souris." Phd thesis, Université de Grenoble, 2011. http://tel.archives-ouvertes.fr/tel-00721763.
Full textAbstract:
Le travail de cette thèse est méthodologique centré autour de l'imagerie du tenseur de diffusion par résonance magnétique (DTI) avec des développements incluant des simula- tion Monte Carlo des signaux RMN de diffusion dans des modèles géométriques du tissu cérébral et des acquisitions de séquence DTI haute résolution 3D pour aboutir à l'imagerie "fibre tracking" dans le cerveau de souris. Différents modèles géométriques de la substance blanche, grise ou encore incluant les deux structures sont proposés. Le principe de leur génération est décrit ainsi que la gestion des contraintes liées à la compartimentation. Il est montré aussi le principe de l'élaboration d'un nouveau "modèle composite" qui per- met de modéliser des géométries complexes comme le mélange de différentes structures ou encore des différentes orientations des axes des fibres de la substance blanche. Un avantage au modèle composite est sa simplicité et le gain en temps de calcul. Les résul- tats des simulations ont été confrontés à des données in vivo et ont permis d'interpréter l'origine des signaux et d'évaluer l'impact de certains paramètres géométriques sur ces signaux. Ils ont permis aussi d'optimiser les conditions d'acquisition. Une application majeure des développements de l'imagerie 3D haute résolution est son application dans un modèle apparenté à la schizophrénie chez la souris. Par comparaison à des souris nor- males, il est montré, pour la première fois en utilisant DTI et l'imagerie fibre tracking, une baisse du volume de la matière blanche et une réduction, voire une absence de la partie post-commissurale du fornix, un tract qui relaie l'hippocampe vers le corps mamillaire.
13
VILLENA, PHILIPPE. "Traitement laser des affections prostatiques : etude de faisabilite en imagerie 2d et 3d." Reims, 1993. http://www.theses.fr/1993REIMM050.
Full text
14
Balocco, Simone. "3D dynamic ultrasonic model of pathologic artery : application to the assessment of arterial wall mechanical parameters." Lyon 1, 2006. http://www.theses.fr/2006LYO10145.
Full textAbstract:
Le travail de recherche porte sur un modèle mathématique multiphysique 3D qui reproduit le comportement biomécanique des vaisseaux humains et leur imagerie échographique. Le modèle, dont la géométrie est basée sur un modèle multi couches, permet la représentation de différentes structures de vaisseau, y compris les sténoses, les bifurcations et les pathologies associées. L'écoulement du sang est simulé en considérant un déplacement dynamique des diffuseurs et un modèle mécanique permet de calculer le déplacement des parois soumises à la pression artérielle à l'aide d'une méthode par éléments finis. Une caractérisation acoustique de chaque région est ensuite réalisée permettant, l'obtention des signaux radiofréquences d'une observation échographique. Le travail de rechereche porte sur la réalisation de deux applications pricipales, représentent respectivement un utile pour la simulation et un utile pour l'aide à la diagnostiqueA mathematic multiphysics 3D model reproducing the biomechanical behavior of human vessels and the related echographic imaging has been developed. The geometry of the model is a multi layer structure based on right generalized cylinders (RGC) and enabling the representation of pathological and healthy vessel structures (stenoses and bifurcations). The blood flow has been simulated considering a dynamical displacement of the erythrocytes and a mechanical model enables the computation of the arterial wall pulsation due to the hydraulic flow pressure. An acoustic characterization of each region has been performed, and radiofrequency signals has been obtained in order to reproduce ultrasound images. The research project has been focused on two main application which represent respectively a simulation and a dignostic support tools
15
Colin, Arnaud. "Etude de methodes de recalage et de fusion d'images 3d du cerveau ; application au suivi d'une pathologie cerebrale." Clermont-Ferrand 1, 1997. http://www.theses.fr/1997CLF1MM13.
Full text
16
Kirchhöfer, Daniel [Verfasser], and Bernd [Akademischer Betreuer] Jähne. "Effiziente Verarbeitungskette für 3D Bilddaten unter Berücksichtigung des Bildaufnahmerauschens am Beispiel der Pathologie / Daniel Kirchhöfer ; Betreuer: Bernd Jähne." Heidelberg : Universitätsbibliothek Heidelberg, 2021. http://d-nb.info/1239116225/34.
Full text
17
"Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Imaging for Accurate 3D Digital Pathology." Tulane University, 2020.
Find full textAbstract:
archives@tulane.eduFor decades, histopathology and cytology have provided the reference standard for cancer diagnosis, prognosis prediction and treatment decisions. However, they are limited to 2D slices, which are created via cutting and/or smearing, thus not faithfully representing the true 3D structures of the cellular or tissue material. Multiple imaging methods have been utilized for non-destructive histologic imaging of tissues, but are usually limited by varying combinations of low resolution, low penetration depth, or a relatively slow imaging speed, and all suffer from anisotropic resolution, which could distort 3D tissue architectural renderings and thus hinder new work to analyze and quantify 3D tissue microarchitecture. Therefore, there is a clear need for a non-destructive imaging tool that can accurately represent the 3D structures of the tissue or cellular architecture, with comparable qualities and features as traditional histopathology. In this work, dual-view inverted selective plane illumination microscopy (diSPIM) has been customized and optimized for fast, 3D imaging of large biospecimens. Imaging contrast of highly scattering samples has been further improved by adding confocal detection and/or structured illumination (SI) as additional optional imaging modes. A pipeline of dual-view imaging and processing has also been developed to achieve more isotropic 3D resolution, specifically on DRAQ5 and eosin (D&E) stained large (millimeter to centimeter size) biopsies. To determine the impact of 3D, high-resolution imaging on clinical diagnostic endpoints, multiple prostate cancer (PCa) biopsies have been collected, imaged with diSPIM, and evaluated by pathologists. It has been found that the pathologist is “equally” confident on the PCa diagnosis from viewing 3D volumes and 2D slices, and the diagnostic agreement between 3D volumes is significantly higher than 2D slices. The high-resolution and large-volume coverage of diSPIM may also help verify results from other lower-resolution modalities by serving as a 3D histology surrogate. Tissue correlations have been found between images acquired by diSPIM and photo-acoustic imaging, or by diSPIM and biodynamic imaging, proving diSPIM as a useful tool to aid in validation of lower-resolution imaging tools. The potential of diSPIM imaging has also been demonstrated in other applications, such as in the study of in-vitro neural models.
1
Bihe Hu
18
Lochovská, Kateřina. "Vznik lidských zubních anomálií na modelu odontogeneze u myší s poruchami genů." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-312521.
Full textAbstract:
Laboratory mouse is a appropriate experimental model for studies on normal tooth development mechanisms and for understanding of etio-pathogenesis of dental anomalies in humans. Mouse dentition consists of one incisor separated from three molars by toothless diastema in each jaw quadrant. Although an adult mice diastema does not contain teeth, rudimentary tooth primordia (MS, R2) appear here during embryonic development. However, their development stops and consequently functional tooth does not arise here. It is known that in mice with mutations in Spry2 and Spry4 genes, supernumerary teeth arise in antemolar region. The understanding of the temporo-spatial dynamics of signaling centers in Spry2/Spry4 double transgenic mice may help to elucidate the role of these embryonic diastemal buds in supernumerary tooth formation. The research of possible regeneration of dental anomalies in off-spring of crossing Spry4 deficient mice with supernumerary teeth formation with Eda deficient mice with predisposition to reduced dentition could be the way to elucidating of mechanism of dental pathologies development in general. Our aim was to describe temporal and spatial dynamics of Shh signaling domains in Spry2/Spry4 embryos, because Shh is one of the markers of early odontogenesis and determining of...
19
Perry, Jamie. "Magnetic resonance imaging and 3D computer modeling of the levator veli palatini muscle before and after primary palatoplasty /." 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3301270.
Full textAbstract:
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007.Source: Dissertation Abstracts International, Volume: 69-02, Section: B, page: 0981. Adviser: David Kuehn. Includes supplementary digital materials. Includes bibliographical references (leaves 123-127) Available on microfilm from Pro Quest Information and Learning.