Dissertations / Theses on the topic '3D organoid'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic '3D organoid.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Johansson, Seiko. "Patient-derived organoid culture for 3D culture of colorectal cancer, renal cancer and osteosarcoma." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-391043.
Full textTOSCANI, CECILIA. "UNCOVERING EPIGENETIC VULNERABILITIES IN INTESTINAL CANCER DEVELOPMENT." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/697052.
Full textGodart, Matthias. "Interactions fonctionnelles entre voies signalétiques intrinsèques et voie des hormones thyroïdiennes dans les cellules souches et progéniteurs de l'épithélium intestinal." Thesis, Lyon, 2019. http://www.theses.fr/2019LYSE1138.
Full textThyroid hormones (THs) control several aspects of gut development and homeostasis. They act through the thyroid hormone nuclear receptors (TRs) that are T3-modulated transcription factors. The paradigm is the amphibian metamorphosis, where they are responsible for gut remodeling and emergence of the stem cells (Ishizuya-Oka et al, 2009). In previous studies we showed that THs play a fundamental role in regulating the balance between cell proliferation and cell differentiation of the murine intestinal epithelial precursors. From a molecular point of view the nuclear receptor TRα1 controls several proliferation/cell-cycle genes as well as the Wnt and Notch pathways (rev. in Sirakov et al, 2104; Skah et al, 2017). In accordance with these functions, targeted expression of TRα1 in the intestinal epithelium (vil-TRα1 mice) is sufficient to induce aberrant and hyper-proliferative crypts and confers increased susceptibility to Apc-mutation dependent intestinal tumorigenic program (vil-TRα1/Apc+/1638N mice) (Kress et al, 2010). The aim of my work was to study TH- and TRα1-dependent control of intestinal stem cells. Indeed, I used the Lgr5-EGFP-ires-CreERT2 mice enable tracking, sorting and targeting the stem cells (Barker et al, 2007) crossed with tamoxifen inducible TRα1 loss-of-function (Quignodon et al, 2007) mouse model (TRα1-LOF). I studied the effect of TH/TRα1 alteration in vivo and in intestinal organoids (ex vivo). In conclusion, our results indicate that HTs and modulating TRα1 expression or activity have a rapid and strong effect on the intestinal stem cells. This work opens a new perspective in the study of TH/TRα1-dependent signal on the physiopathology of the intestinal stem cells
Dinelli, Giulia <1993>. "Matrici Naturali e Sintetiche per colture 3D di Organoidi." Master's Degree Thesis, Università Ca' Foscari Venezia, 2019. http://hdl.handle.net/10579/15246.
Full textWorkman, Michael J. "Generating 3D human intestinal organoids with an enteric nervous system." University of Cincinnati / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416570664.
Full textBagnara, Elena <1993>. "Evaluation of synthetic alternatives scaffold for 3D culture method for liver organoids." Master's Degree Thesis, Università Ca' Foscari Venezia, 2021. http://hdl.handle.net/10579/20090.
Full textAnderle, Marica. "Human cerebellar organoids as an in vitro 3D model of Group 3 Medulloblastoma." Doctoral thesis, Università degli studi di Trento, 2020. http://hdl.handle.net/11572/262782.
Full textMa, Lin. "Multi-scale 3D imaging of the microstructure in organic-rich shales." Thesis, University of Manchester, 2016. https://www.research.manchester.ac.uk/portal/en/theses/multiscale-3d-imaging-of-the-microstructure-in-organicrich-shales(514544f2-39f8-4fe2-b8f4-fecb27380c10).html.
Full textLangner, Maik. "Laterally modified microcavity systems containing organic emitters." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-67568.
Full textLi, Qian. "Organic Semiconductors Based on Triazastarphene Towards 3D : Charge Transport in Crystalline Phase." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0144.
Full textThe research in organic electronics has attracted worldwide attention due to the specific properties of organic materials such as lightness, flexibility, large scale processing ability and low production cost. Compared with inorganic materials, the strong anisotropy and low charge carrier transport mobility limit their integrations in commercial devices. This research aims at developing molecule systems leading to three-dimensional charge transport.To reach this goal, our design strategy is to expand the generally linear-shaped molecular structure into star-shaped structure with C3h symmetry. The designed molecules consist of a planar core of fused aromatic cycles to form efficient - stacking with neighboring molecules and of bulky groups located close to the center to increase the solubility of the materials and prevent 1D columnar packing. Thus, two supramolecular arrangements are speculated (arm and column packing) where charge hopping follows pathways in 3D.Based on this design, 13 triazastarphenes substituted directly by amine or phenyl groups were synthesized and thoroughly characterized. Their electronic properties were carefully determined by UV-visible absorption spectroscopy, cyclic voltammetry and DFT calculations. Results from single crystal XRD showed that the experimental packing is similar to model for few molecules. In detail, one amino-triazastarphene has shown a new 2D layer by layer packing motif, while benzo-triazastarphenes have led to column packing in one case and half arm packing for another as expected. In addition, theoretical approach highlighted 2D and 3D dimensionality for charge carrier transport for the two later examples in the crystal phase.Finally, this work is the first report about straightforward synthesis of extended C3h acridine derivatives. Even though the performances obtained from the devices (OFETs and perovskite solar cells) based on these materials did not reach state of the art performances, the novel synthetic method and the achievement of interesting molecular arrangement motifs in single crystal can contribute to the development of high-performance OSCs
Nassor, Férid. "Etude de mécanismes de type prion impliqués dans la maladie d’Alzheimer sur un modèle de mini-cerveaux humains avec exploration par microscopie 3D." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS105.
Full textHuman neurodegenerative diseases, which are all based on prion-like mechanisms (self-propagation of pathogenic protein aggregates), represent a major societal risk with the increase of their prevalence directly correlated to the increasing longevity of the world population. There is to date neither any cure nor any pertinent experimental model for their study.The objective of this work was to use the potential of human mini-brains, a self-assembled three-dimensional in vitro model able to restitute the complexity of the cerebral cortex. This model will allow us to study prion-like mechanism by developing a validation methodology based on 3D microscopy. These novel 3D structures can be obtained from reprogrammed adult cells into induced pluripotent stem cells (iPSCs) and offer unique possibilities to access, observe and disrupt biological processes in the human brain without bias nor complications as in animal models and ex vivo human brain samples. This model makes it possible to observe the development of aggregates of Aβ and hyper-phosphorylated Tau, two proteins that accumulate and propagate from cell to cell during Alzheimer’s disease.We have been to able to adapt the cerebral organoid model for a future screening approach by modifying the differentiation methodology to enhance its production ratio. We also have been able to test different modalities for disease modeling for Alzheimer’s disease, Parkinson’s disease, Fronto-Temporal Dementia and Creutzfeldt-Jakob disease: with chemical induction, with patient specific cells, through genetic modification and through contact with infectious material. These different approaches allowed us to validate that the cerebral organoid can indeed reproduce key aspects found during pathological development within patients. To compensate for the heterogeneity of the cerebral organoid, we performed an in toto analysis through microscopy, meaning in its totality without prior slicing. The chosen method of acquisition is fluorescence light-sheet microscopy used after staining and optical clearing of cerebral organoids. To do so, we have evaluated and established different strategies in order to obtain a high content screening platform for our cerebral organoid model.This platform centered around the cerebral organoid model, underpinned by 3D microscopy analysis, was developed during the “Investissements d’Avenir” project 3DNeuroSecure. This project has for ambition to bring high performance computing to the biological sciences, notably with the possibility to deal with large scale data, also called “exascale”, like the ones obtained with 3D microscopy. The development of this aspect would allow us to establish the basis for a therapeutic screening tool based on cerebral organoids for neurodegenerative diseases. We have demonstrated that prion-like mechanisms can be studied in a human mini-brain model and multiple research avenues are now opened for both fundamental and applied research. This platform could in turn become the basis for any kind of organoids derived from patients to model the whole human body and become a biological companion for personalized medicine
Senevirathna, Wasana. "Azadipyrromethene-based Metal Complexes as 3D Conjugated Electron Acceptors for Organic Solar Cells." Case Western Reserve University School of Graduate Studies / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1402062085.
Full textMyszczyszyn, Adam. "Studying normal and cancer stem cells in the kidney using 3D organoids and genetic mouse models." Doctoral thesis, Humboldt-Universität zu Berlin, 2021. http://dx.doi.org/10.18452/23127.
Full textAdult mouse organoids are promising models for kidney research. However, their characterization has not been pushed forward to a satisfying level. Here, I have generated and characterized a long-term 3D mouse organoid (tubuloid) model, which recapitulates renewal and repair, and the architecture and functionality of the adult tubular epithelia. In the future, the model will allow detailed investigations of trajectories of self-renewing cells towards both the partial recreation and malignant transformation of the kidney. Clear cell renal cell carcinoma (ccRCC) is the most common and aggressive kidney cancer. Inactivation of the Von Hippel-Lindau (VHL) tumor suppressor gene is the major driver of ccRCC. Earlier, we identified the upregulation of Wnt and Notch signaling in CXCR4+MET+CD44+ cancer stem cells (CSCs) from primary human ccRCCs. Blocking Wnt and Notch in patient-derived xenografts, organoids and non-adherent spheres using small-molecule inhibitors impaired self-renewal of CSCs and tumor growth. To mimic CSC-governed human ccRCC in genetic mouse models, I started from the generation of two double mouse mutants; β-catenin-GOF; Notch-GOF and Vhl-LOF; β-catenin-GOF. Surprizingly, I observed neither tumors or tumor precursor lesions nor higher cell proliferation rates in the mutant kidneys. Further analyses revealed that the mutant mice displayed features of chronic kidney disease (CKD). Thus, β-catenin-GOF; Notch-GOF and Vhl-LOF; β-catenin-GOF mouse mutants did not develop kidney tumors under the given experimental conditions.
Saini, Anuj. "FROM APPLICATION OF ORGANIC THIN MULTILAYER FILMS IN 3D OPTICAL DATA STORAGE TO THEIR FABRICATION FOR ORGANIC ELECTRONIC DEVICES." Case Western Reserve University School of Graduate Studies / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=case1459518551.
Full textSébert, Morgane. "Les effets de la thrombine sur l'épithélium colique humain, grâce aux organoïdes (modèle ex vivo, 3D)." Thesis, Toulouse 3, 2018. http://www.theses.fr/2018TOU30006/document.
Full textThrombin, a serine protease known for its role in the coagulation cascade, was described for its effects on the induction of apoptosis in colonic epithelial cell lines, through the activation of Protease Activated Receptors (PARs). However, the effects of thrombin on complex epithelial structures such as the human intestinal epithelium composed of different cell types and cells at different stages of differentiation, has never been investigated. A new cellular model, named organoid, enables to reconstitute a functional epithelium in 3-dimensions (3D), from human resections or biopsies, thanks to the self-renewal and differentiation properties of stem cells isolated from colonic crypts. The first objective of this thesis was to evaluate thrombin's effects on survival, proliferation, apoptosis and differentiation in human colonic epithelium, using the organoid model. Then, we aimed to determining the implication of PAR1 and PAR4 in the thrombin's effects. Thus, thrombin added (at low dose: 10mU/mL and higher dose: 50mU/mL) to organoid cultures from control patients, led to a decrease by half of metabolic activity and cell proliferation. These effects were blocked by the addition of a PAR1 antagonist. Apoptotic process was 8-fold higher in organoid cultures exposed to thrombin (both doses) and this effect was inhibited by the addition of a PAR1 or a PAR4 antagonist. As per epithelial differentiation, thrombin decreased the number of colonospheres (immature structures) favoring the increase of apoptotic structures and colonoids (budding structures considered as more mature). This effect was due to PAR1 and PAR4 activation as again, it was blocked both by PAR1 and PAR4 antagonist. Taken together, these results reveal that exogenous thrombin acts on apoptosis, proliferation and differentiation processes in complex human colonic epithelium. The use of this ex vivo model will allow to compare pathological versus normal organoid cultures, but also to test the effects of pharmacological approaches and new treatment options directly in cultured human tissues. Thus, the 2nd part of this thesis was to setup the best culture conditions and the best imaging conditions to perform a robust and reproducible screening approach, HCS (High-Content Screening), using organoid cultures. Culture conditions in 96-well plates were set up and allowed to acquire images with the HCS system. Operetta HCS coupled to an analysis software (Harmony, PerkinElmer) was used to develop a specific program enabling the recognition of organoids, their counting, their classification according to their differentiation status and enabling to follow organoid growth in cultures. To sum up, the work performed allowed to highlight the effects of thrombin on metabolic status, apoptosis and differentiation of human colon epithelium, using an ex vivo 3D organoid model. The use this model nicely completed epithelial cell line approaches, offering an integrated view of the complex behavior of human epithelium. The HCS approach initiated within this thesis should allow the automated analysis of a number of drugs and treatments. It should help our understanding of epithelial pathologies and the testing of new therapeutic approaches
Smolka, Rastislav. "Luminiscenční nanočástice pro 3D zobrazování." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-449374.
Full textKearns, Eleanor Rose. "Multi-stimuli Metal-organic frameworks and their composites." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/29561.
Full textMyszczyszyn, Adam [Verfasser]. "Studying normal and cancer stem cells in the kidney using 3D organoids and genetic mouse models / Adam Myszczyszyn." Berlin : Humboldt-Universität zu Berlin, 2021. http://d-nb.info/123964471X/34.
Full textPriadi, Eka. "Behaviour of Tiang Tongkat foundation over Pontianak soft organic soil using 3D-finite element analysis." Doctoral thesis, Technische Universitaet Bergakademie Freiberg Universitaetsbibliothek "Georgius Agricola", 2009. http://nbn-resolving.de/urn:nbn:de:bsz:105-081293.
Full textAnhorn, Michael J. "Nitrogen Rich Porous Organic Frameworks: Proton Conduction Behavior of 3D Benzimidazole and Azo-linked Polymers." VCU Scholars Compass, 2018. https://scholarscompass.vcu.edu/etd/5448.
Full textForlivesi, Claudio. "Biomateriali e 3D bioprinting nella rigenerazione neurale." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2019. http://amslaurea.unibo.it/17888/.
Full textHume, Robert David. "Recapitulating mammary gland development and breast cancer cell migration in vitro using 3D engineered scaffolds." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/271099.
Full textGarcia, Jordan. "DESIGN AND PROCESS OF 3D-PRINTED PARTS USING COMPOSITE THEORY." UKnowledge, 2019. https://uknowledge.uky.edu/me_etds/144.
Full textLafargue, Clément. "Dynamique non-linéaire dans les microcavités laser tridimensionnelles à base de polymères : aspects physiques et technologiques." Phd thesis, École normale supérieure de Cachan - ENS Cachan, 2013. http://tel.archives-ouvertes.fr/tel-00920272.
Full textSalaün, Mathieu. "Croissance d'oxyde métallique par photolyse dans un réseau d'interférences 3D." Phd thesis, Grenoble INPG, 2008. http://tel.archives-ouvertes.fr/tel-00612071.
Full textNur, Hassan Mohammed. "Fabrication of advanced ceramics and selective metallization of non-conductive substrates by inkjet printing." Thesis, Brunel University, 2002. http://bura.brunel.ac.uk/handle/2438/4823.
Full textVazquez, Armendariz Ana Ivonne [Verfasser]. "Establishment of murine 3D bronchioalveolar lung organoids from adult somatic stem cells for organ development and disease modeling / Ana Ivonne Vazquez Armendariz." Gießen : Universitätsbibliothek, 2018. http://d-nb.info/1170582028/34.
Full textLacombe, Elsa. "Évaluation de la technologie photonique sur silicium pour le développement de liens sans fil innovants visant 40 Gb/s au-delà de 200 GHz." Thesis, Université Côte d'Azur (ComUE), 2018. http://www.theses.fr/2018AZUR4095/document.
Full textWith the booming of mobile data traffic, the need for higher data-rates is clearly felt. To cope with this strong demand and support the 5G roll-out, the capacity of the mobile communication network is being improved every day with many solutions, among which the development and installation of millimeterwave (mmW) wireless systems operating at up to 10 Gb/s. However, in order to deliver such high speeds to the user, the fronthaul/backhaul network sending data back to the core network would require above 40 Gb/s data-rate wireless links. This challenge generates a growing interest for sub-mmW and THz frequencies (0.1 THz – 1 THz) at which up-to 100 GHz bandwidth (BW) is accessible. In such BW, it would be possible to achieve up to 100 Gb/s data-rates while using simple modulation schemes to reduce the wireless system’s power consumption. Targeting mass-market high data-rates applications, Silicon Photonics technology seems very promising as it benefits from wide intrinsic BW and powerefficient components, as well as high integration levels and low manufacturing costs. In this context, a main aspect of this PhD project is the evaluation of an industrial Silicon Photonics technology for the development of a THz system-on-chip transmitter capable of reaching up to 100 Gb/s using a photodiode. Since THz antennas are also a hot topic for THz point-to-point transmission, a second aspect of this PhD study is the design of a low-cost and compact THz antenna-system. Hence, a planar antenna using low-loss organic packaging technology and a 3D-printed plastic lens were developed in order to assess those industrial prototyping techniques above 200 GHz
Grosser, Gary [Verfasser]. "Der Sodium-dependent Organic Anion Transporter SOAT : Gewebeexpression, vergleichende funktionelle Charakterisierung und Generierung einer 3D-QSAR Pharmakophore / Gary Grosser." Gießen : Universitätsbibliothek, 2013. http://d-nb.info/1064991521/34.
Full textHamida, Youcef. "MAGNETISM IN A NUMBER OF METAL ORGANIC FRAMEWORKS (MOFs) WITH 1D AND 3D CHARACTERISTICS: AN EXPERIMENTAL AND ANALYTICAL STUDY." Diss., Temple University Libraries, 2012. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/195069.
Full textPh.D.
Metal Organic Frameworks (MOFs) exhibit many excellent physical properties including magnetic properties for potential applications in devices. More importantly for the subject of this thesis, MOFs are ideal for the realization of low dimensional magnetism because of the large selection of ligands connecting magnetic centers in making the framework. The materials studied in this thesis include ten magnetic MOFs of the form M(L1)(L2) [M = Cu, Ni, Co, Fe, Mn; L1 = NDC, bpdc, BDC, BODC, N3; L2 = DMF, H2O, TED, bpy]. Polycrystalline powder samples as well as single crystal samples were synthesized. Their crystal structures were determined, and their magnetic and thermodynamic properties were measured and analyzed. Eight of these materials were characterized as 1D magnets and two as 3D magnets. In the 1D case it is found that above Tm [the temperature at which the magnetic susceptibility χ(T) has a peak] the magnetic behavior of MOFs (S ≥ 1) can be well described with the Classical Fisher Model (CFM). Near and below TC the spins take a more definite orientation than allowed for in the CFM and hence the Ising Model (IM) was used for fitting. Both CFM and IM yield fairly consistent intrachain couplings (J) when applied in their appropriate temperature region. To estimate the interchain exchange (J′), the susceptibility for a magnetic chain in the mean field of neighboring chains is used. In all cases, as expected, the ratio of J to J′ was less than 10%. The special case of Cu(N3)2bpy (S = ½) was analyzed with the spin ½ IM. Although the specific heat data (Ctotal) for most of the 1D MOFs showed no clear phase transition, a low temperature fit to the electron-phonon specific heats yielded apparent heavy fermion-like &gamma values on the order of several hundred mJ/mol K2. The lattice specific heat (C lattice) was estimated using a Debye-Einstein hybrid model. Subtracting Clattice from Ctotal, magnetic specific heat (CM) with a broad peak characteristic of low dimensional magnetism was obtained. The peak in CM was at temperature near that expected from χ(T) fits. The J values obtained from the magnetic specific heat fits were in good agreement with those obtained from χ(T) fits. Once the magnetic specific heat was accounted for, γtakes values in the expected range of few mJ/mol K2. For 3D MOFs [Mn(N3)2bpy and Fe(N3)2bpy], the existence of long range canted antiferromagnetic ordering was observed in both magnetic and specific heat measurements with phase transitions at 38 K and 20 K in the case of Mn(N3)2bpy and Fe(N3)2bpy, respectively. These transition temperatures are considered fairly high for molecular based materials. In both Mn(N3)2bpy and Fe(N3)2bpy, the χ(T) data fit well to the Heisenberg model for a diamond-type network. The transition can clearly be seen with an abrupt increase in the magnetization below TC and a shift to a higher temperature in the specific heat when measured under an applied magnetic field. The systematic approach in this work led to the successful estimate of C lattice resulting in meaningful fitting of χ(T) and Cmagnetic to the appropriate theoretical models in magnetism. It also led the discovery of ferrimagnets or canted antiferromagnets M(N3)2bpy with large coercivity and rather high transition temperature. The results of this study have been published in three articles in the Journal of Applied physics, and two manuscripts are under preparation for submission [1-5].
Temple University--Theses
Hauptvogel, Ines Maria, Volodymyr Bon, Ronny Grünker, Igor A. Baburin, Irena Senkovska, Uwe Müller, and Stefan Kaskel. "A family of 2D and 3D coordination polymers involving a trigonal tritopic linker." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-138965.
Full textDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich
Hauptvogel, Ines Maria, Volodymyr Bon, Ronny Grünker, Igor A. Baburin, Irena Senkovska, Uwe Müller, and Stefan Kaskel. "A family of 2D and 3D coordination polymers involving a trigonal tritopic linker." Royal Society of Chemistry, 2012. https://tud.qucosa.de/id/qucosa%3A27797.
Full textDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
Palacios, Sebastian R. "A smart wireless integrated module (SWIM) on organic substrates using inkjet printing technology." Thesis, Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/51906.
Full textBASTA, Luca. "Defect-engineered graphene functionalization via cycloaddition reaction – towards a versatile platform for nanoscale devices and 3D heterostructures." Doctoral thesis, Scuola Normale Superiore, 2022. https://hdl.handle.net/11384/126242.
Full textBaillie-Johnson, Peter. "The generation of a candidate axial precursor in three dimensional aggregates of mouse embryonic stem cells." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/267818.
Full textBossard, Cédric. "Elaboration et caractérisation d’un hybride organominéral à base de polycaprolactone et de bioverre sous forme de mousse macroporeuse pour la régénération osseuse." Thesis, Université Clermont Auvergne (2017-2020), 2018. http://www.theses.fr/2018CLFAC068/document.
Full textThe increase in life expectancy results in the decline of seniors’ health conditions and the resurgence of chronic diseases. Among the expressions of senescence, disorders of the musculoskeletal system are particularly disabling and considerably accelerate the state of dependency. This is also the case for young people who suffer from traumatic injuries or pathologic conditions. Thus, about 2.2 million bone grafts are performed worldwide every year. Yet, the level of postoperative complications remains high and is estimated at 15% of surgical operations. These facts outline a major societal concern: animal-based materials present a risk of histocompatibility issues and pathogenicity that may lead to implant failure. This is the reason why research efforts focus on the development of synthetic biomaterials capable of promoting bone regeneration. Currently, commercialised bone substitutes are mainly made of bioactive “ceramics” (calcium phosphates, bioactive glass) that are known to be biocompatible, to spontaneously bond to bone tissues, to promote bone cell adhesion and finally to be bioresorbable. However, despite these remarkable properties, the brittleness of these materials limits their applications. An ingenious solution to this brittleness can be learned from the particular structure of bone tissue. Bone tissue intimately blends an inorganic phase, the bone mineral, which is made of apatite crystals (resorbable calcium phosphates), with an organic phase that is mainly collagen. Such a structure associates the stiffness of the inorganic part with the toughness of collagen fibres. Therefore, in order to obtain implants with mechanical properties similar to that of bone, the strategy consists in combining bioactive ceramics with organic matter. To this end, the Biomatériaux team from the Laboratoire de Physique de Clermont (LPC) recently developed an innovative process that allows the synthesis of tridimensional organic-inorganic hybrids comprised of bioactive glass and biocompatible polymer. The objective of the thesis was to exploit this process in order to develop a hybrid bone substitute with optimal properties. First of all, polycaprolactone was selected as the polymer, especially because of its adequate degradation rate for long-term applications such as bone regeneration. Then, the synthesis process was improved (in particular, the calcium source was changed), the macroporous structure was optimised and the organic-inorganic ratio was chosen. Afterwards, elements that are known to induce an osteogenic effect were incorporated in the hybrid at low doses (< 5% of total weight): an inorganic doping was performed using strontium ions and an organic doping was performed using nutrients such as fisetin or hydroxytyrosol. The resultant hybrid scaffolds were eventually characterised in vitro in order to determine their physicochemical and biological properties and in vivo in order to evaluate their performance. After 3 months of implantation in a mouse calvarial critical defect, results demonstrate the potential of this bone substitute: compared to the reference commercial material (treated bovine bone) that leads to a bone reconstruction of 16% (± 5%), the hybrid allows a reconstruction going from 32% (± 3%) when it is not doped, to 55% (± 7%) and even 58% (± 7%) when it is doped respectively with fisetin or strontium. This thesis paves the way to promising perspectives like the association of doping agents and the 3D printing of polycaprolactone-bioactive glass hybrid scaffolds
Zhao, Huiyu. "Analyse de la matière organique et ses propriétés dans l’environnement naturel en spectroscopie de fluorescence 3D traitée par PARAFAC." Thesis, Toulon, 2011. http://www.theses.fr/2011TOUL0003/document.
Full textFluorescence excitation and emission matrices (EEM) are used to characterize natural organic matter (NOM). To make best use of this information, PARAFAC, a trilinear algorithm is employed. After removing Rayleigh and Raman scattering and correction of the inner filter effect, this method allows separating the spectral components present in MEEF.This work presents two studies: the characterization and quantification of NOM according to its origin and calculation of environmental complexation constants of NOM towards copper as ionic form.Spectral components and their relative intensities are calculated by PARAFAC from 1146 samples gathered according to the missions, the medium type, or the salinity level. To study these components, a new spectral representation is proposed in order to highlight their spectral variability. The results show that even when samples spectra of various origins are clustered, the overall spectral overlap and the relative intensities remain almost similar. On the whole spectral range, areas corresponding to humic substances are quite variable, compared to the protein zone.Metal complexation by NOM is analyzed by combination of four tools: metal ions logarithmic addition, MEEF measurement, the PARAFAC dissociation method and the PROSECE modelling algorithm. Fluorescence quenching measurement is not only limited to the modelling of fluorescence intensity but also to the relative intensity of each PARAFAC-dissociated component though surpassing the methods used so far. Finally, the application of this improving technique leads to quantify NOM complexation properties using a two-complexing sites complexation model for each PARAFAC-dissociated component by using the whole fluorescence signal
Simpson, Emily. "Development and characterization of two versions of a new single particle mass spectrometer for organic aerosol analysis that incorporate a 3D ion trap." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/17414.
Full textBruns, Benjamin Verfasser], Ralf [Akademischer Betreuer] [Littke, and Peter A. [Akademischer Betreuer] Kukla. "Unconventional petroleum systems in NW-Germany and the Netherlands : a 3D numerical basin modeling and organic petrography study / Benjamin Bruns ; Ralf Littke, Peter A. Kukla." Aachen : Universitätsbibliothek der RWTH Aachen, 2016. http://d-nb.info/112978746X/34.
Full textRippen, Daniel Verfasser], Ralf [Akademischer Betreuer] [Littke, and Brian [Akademischer Betreuer] Horsfield. "Oil and gas shales of Northern Germany : implications from organic geochemical analyses, petrophysical measurements and 3D numerical basin modelling / Daniel Rippen ; Ralf Littke, Brian Horsfield." Aachen : Universitätsbibliothek der RWTH Aachen, 2015. http://d-nb.info/1130327094/34.
Full textLee, Darryl Liang Wee. "Development of a PP-MOCVD System and its Design and Operational Parameters for Uniform Industrial Coatings on 3D Objects." Thesis, University of Canterbury. Dept. of Mechanical Engineering, 2014. http://hdl.handle.net/10092/9644.
Full textSengal, Asmerom Tesfamariam. "Prognostic, predictive, and therapeutic role of FGFR2 isoforms and cognate FGF ligands in endometrial cancer." Thesis, Queensland University of Technology, 2020. https://eprints.qut.edu.au/205851/1/Asmerom%20Tesfamariam_Sengal_Thesis.pdf.
Full textJenson, Sage. "Digital Morphologies: Environmentally-Influenced Generative Forms." Oberlin College Honors Theses / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=oberlin1499763863308499.
Full textNun, Nicholas. "Improving Skin Wound Healing Using Functional Electrospun Wound Dressings and 3D Printed Tissue Engineering Constructs." University of Akron / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1617985844538101.
Full textRichardson, Kevin Thomas. "DESIGN AND ANALYSIS OF A 3D-PRINTED, THERMOPLASTIC ELASTOMER (TPE) SPRING ELEMENT FOR USE IN CORRECTIVE HAND ORTHOTICS." UKnowledge, 2018. https://uknowledge.uky.edu/me_etds/127.
Full textLoizeau, Vincent. "Prise en compte d'un modèle de sol multi-couches pour la simulation multi-milieux à l'échelle européenne des polluants organiques persistants." Thesis, Paris Est, 2014. http://www.theses.fr/2014PEST1168/document.
Full textPersitent Organic Pollutants (POPs) are toxic substances that bioaccumulate in the food chain. Once emitted in the atmosphere, they are transported by the wind and deposited on soil. Since they are persistent, they can be reemited from soil to atmosphere by volatilization and travel over very long distances. This process is called grasshopper effect. Thus, POPs may be found at significant levels far from their emission source. It is necessary to understand the transport and fate of these pollutants in order to support the decision making process and reduce human exposure to POPs. Regulations over the last decades lead to a decrease of anthropogenic emissions and subsequent decrease of atmospheric concentration. In this context, the soil is no longer a sink of POPs but can be a source to the atmosphere. Many numeric models aim to study the behavior of POPs in the environment. Most of them consider soil compartment as a homogeneous box, leading to an underestimation of reemissions. Then, it appears of great importance to develop more realistic soil models. The objective of my thesis was to develop such a model, with vertical transport within the soil. This model was evaluated against measured concentration soil profile. We also conducted a sensitivity analysis to identify the key parameters involved in the process of reemissions. Then, the soil model was coupled with an atmospheric transport model. A case study was finally undertaken to estimate the impacts of reemissions on global-mass balance of POPs at European scale
Pop-Piron, Flavia-Florina. "Nouvelles architectures fonctionnelles pour la reconnaissance moléculaire, systèmes conjugués à faible Gap et matériaux pour électrode avancés." Phd thesis, Université d'Angers, 2009. http://tel.archives-ouvertes.fr/tel-01016464.
Full textIshiki, Hamilton Mitsugu. "Relações quantitativas entre estrutura química e atividade biológica (QSAR/QSAR-3D) de compostos com potencial atividade antituberculose." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/46/46135/tde-25042016-172925/.
Full textTuberculosis is an illness caused by Mycobacterium tuberculosis. Data from World Health Organization (WHO) estimates, that about 2-3 millions of human population died by Mycobacterium tuberculosis infection and that during the next 15 years about 1 billion will be infected and 35 million will certainly die. Although, in the clinic it was found several antiTBdrugs, these numbers will increase due several reasons including M. tuberculosis resistant strains. It has been stressed the importance of novel medicines and/or alternative biological targets research projects. It is known that Ribonucleotide reductase (RNR), is an enzyme that catalyses the rate limiting step in the de novo synthesis of dNTPs, reducing the ribonucleoside 5\'-diphosphates to the corresponding deoxyribonuc1eoside 5\' -diphosphates. RNR has a critical role in the DNA synthesis and, hence, cell division. This key enzyme, that shows 16% homology when compared with mammals RNR, is a potential target for drug design of cell growth inhibitors, with potential application in cancer therapy, antimalaria and trypanosome chemotherapy. It is known that different types of compounds or species by means of different mechanism pathways can show RNR inhibition, including α-(N)-heterocyclic carboxaldehydes thiosemicarbazones that are one of the most potent classes of RNR inhibitors. More than that, some of them, that shows activity against M. tuberculosis seems to follow the same mechanism pathways proposed to the thiosemicarbazones tumor cells activity that means, that they probably are RNR inhibitors. In this study, a series of 40 α-(N)-2-formyl-pyridine thiosemicarbazone derivatives tested against RNR of H.ep.-2-cells (human epidermoid carcinoma), taken from selected literature (French & Blanz-Jr. 1974), has quantitatively analyzed by means of several QSAR/3D-QSAR approaches. These compounds were divided into 5 individual subsets, namely A, B, C, D, and E, having 40, 39, 30, 23 e 22 compounds, respectively. This procedure has been done in order to achieve more structurally homogeneous subsets. For each set, three individual training and test sets (I,II and III) have been created in order to evaluate the predictivity power of the generated QSAR/3D-QSAR models. QSAR analysis have been done using descriptors generated by DRAGON program that have been further pre-selected by PLS procedures. Considering that crystallographic data of RNR M. tuberculosis are not available in the literature, 3D-QSAR studies have been done these applying, initially, CoMFA and CoMSIA approaches, implemented in SYBYL. Homology model studies have been performed with WHATIF program CoMFA e CoMSIA approaches used optimized geometry obtained by semi-empirical AM1 methods that have been aligned by two different methods. Rigid alignment, in which the compounds were fitted atom-by-atom onto a template, based on the root mean square fit. The N(l) and C(2) atoms of the pyridine moiety and the heavy atoms of thiosemicarbazone backbone of TSC were used as template structure. (2) Field based, in which the steric and electrostatic fields, generated by the SEAL program were considered in the alignment. In both procedures the unsubstituted 2-formylpyridine thiosemicarbazone in its syn conformation, has been taken as template. Homology RNR models were done using as template crystallographic data of ammoniagenes (1KGN) and S. typhimurium (1R2F) as template, respectively, with identity larger than 65%. More recent1y new crystallographic data have been published for the beta chain (smaller subunity) of RNR do M. tuberculosis (1UZR). CoMFA and CoMSIA generated models showed acceptable predictive correlation coefficients with high fitted correlation coefficients and low standard errors. Betler CoMFA and CoMSIA models have been derived considering a homogeneous subset of TSC substituted only at 5-position in pyridine ring. Reasonable predictive correlation coefficients for CoMSIA models with high fitted correlation coefficients and very low standard errors were obtained. The derived CoMFA and CoMSIA countour maps suggested that a hydrogen bond acceptor near the nitrogen pyridine ring could enhance inhibitory activity value. This observation is in good agreement with literature, in which a complex formation between TSC and iron ion has been suggested, to RNR inhibition. These studies are expected to enhance the understanding of the structural features of this class of TSC-RNR inhibitors as antitumor agents in terms of steric, electrostatic, hydrophobic and hydrogen donor and acceptor fields as well as to contribute to rational design of inhibitors of this key enzyme. Additionally, two compounds that have been prepared by us showed activity against M. tuberculosis using in vivo test system.
Grall, Romain. "Nouvelles stratégies pour le traitement des tumeurs chimio et radio-résistantes : Nanodiamants hydrogénés et Solides hybrides poreux. Etude in vitro dans des systèmes 2D et 3D." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA11T034/document.
Full textThe present work focuses on nanoparticles and their great skills for oncology therapies. Two kinds of nanoparticles have been studied in order to biologically validate and characterize their features. The use of hydrogenated Nanodiamonds (H-NDs) as radio sensitizer is based on a physic-chemical postulate where they act as oxidative stress generator through interaction with irradiation. Thus we validated this hypothesis in radio resistant kidney and breast cancer cell lines and identify senescence as the main pathway after co-treatment with H-NDs and irradiation. Metal organic frameworks are also of particular interest for drug delivery because of their very important loading capacities. Here we demonstrate the biocompatibility of the empty compounds in four lung and hepatic cancer cell lines, a main point before their involvement in drug delivery strategies. Finally, following international guidelines encouraging to make animal testing more ethic, we developed a new 3D cell culture mimicking mucinous lung adenocarcinoma. This well characterized model will be used for the study of cancer development and drug screening
Guigue, Julien. "Influence des facteurs biotiques et abiotiques sur la dynamique de la matière organique du sol à partir de la caractérisation biogéochimique des matières organiques solubles." Thesis, Dijon, 2014. http://www.theses.fr/2014DIJOS052/document.
Full textSoils are the greatest reservoir of C on the continents, and organic matter mineralisation bymicrobial activity represents the major part of the CO2 emitted by terrestrial ecosystems.In this work, we studied water-extractable organic matter (WEOM), which corresponds to themore reactive fraction of soil organic matter (SOM). Our objectives were (i) to identify therelationships of WEOM dynamics with bacterial communities, and with soil physico-chemicalparameters; (ii) to provide a precise chemical characterisation of WEOM.There is a strong link between SOM solubility and the structure of bacterial communities, andan erosion of their diversity has an impact on SOM and WEOM dynamics, and leads to adecrease in organic matter mineralisation. A study at the regional scale then allowed us to identifythat the SOM and clay contents control the quantities of WEOM and its aromaticity. TheWEOM characterisation at the molecular level revealed the presence of a large number ofubiquitous molecules in the WEOM. Based on these analyses, we were also able to describe theeffects of vegetation and soil physico-chemical properties on the chemical composition ofWEOM