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Dissertations / Theses on the topic '3D culture model'

Author: Grafiati

Published: 14 March 2023

Last updated: 25 May 2024

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1

Zhao, Huizhi. "3D Cell Culture Model Synthesized By Polycaprolactone Nanofiber Electrospinning." Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1531319675295094.

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Peddagangannagari, Sreekanth Reddy. "An in vitro human 3D co-culture model to study endothelial-astrocyte interactions." Thesis, Open University, 2012. http://oro.open.ac.uk/54831/.

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At the gliovascular interface, reciprocal inductive influences between brain microvascular endothelial cells (BMVEC) and astrocytes occur. Most of the knowledge in this area of research is derived from in vitro eo-culture models in which astrocytes are cultured on a stiff, two-dimensional (2D) surface. Three-dimensional (3D) culture models closely mimic the in lJZVO cellular architecture and they bridge the gap between 2D culture models and animal models. Hence, an in vitro 3D eo-culture model was developed and characterised, to study the interactions between BMVEC and astrocytes. In this mode

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Smith, Jenny Thompson. "A 3D culture model to investigate cellular responses to mechanical loading in spinal cord injury." Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/16199/.

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Spinal cord injury (SCI) can cause paralysis, loss of sensation, and respiratory dependency, which has a significant impact on the quality of life of patients, their life expectancy and is also a significant economic burden due to the high costs associated with primary care and loss of income. One of the difficulties in establishing a treatment method is the heterogeneity of SCI; there are many different types and severities of traumatic primary injury, across different age groups of patients and different locations within the spinal cord, whilst at a cellular level, there are multiple, intera

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Manzan, Martins Camilla. "EFFECT OF ENDOCRINE DISRUPTORS ON HUMAN ENDOMETRIAL STROMAL CELLS AND THEIR INTERACTION WITH TROPHOBLAST." Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1183943.

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Decidualization is crucial for embryo development and implantation, placenta formation and fetal growth. This process is characterized by morphological and biological changes in endometrial stromal cells that play a key role on fetal trophoblast migration and invasion. Successful placentation depends on the interaction between endometrial stromal cells and extravillous trophoblast cells. The trophoblast spheroids, a 3D culture model, is reported to appropriately mimic the in vivo situation, and reflect the cell to cell interaction. The Bisphenol A (BPA) and para-nonylphenol (p-NP) are endocri

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Sieh, Shirly. "Development of a 3D culture system to study the skeletal metastasis of prostate cancer." Thesis, Queensland University of Technology, 2011. https://eprints.qut.edu.au/50870/1/Shirly_Sieh_Thesis.pdf.

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In the cancer research field, most in vitro studies still rely on two-dimensional (2D) cultures. However, the trend is rapidly shifting towards using a three-dimensional (3D) culture system. This is because 3D models better recapitulate the microenvironment of cells, and therefore, yield cellular and molecular responses that more accurately describe the pathophysiology of cancer. By adopting technology platforms established by the tissue engineering discipline, it is now possible to grow cancer cells in extracellular matrix (ECM)-like environments and dictate the biophysical and biochemical pr

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Vazquez, Marisol. "Development of a novel in vitro 3D osteocyte-osteoblast co-culture model to investigate mechanically-induced signalling." Thesis, Cardiff University, 2013. http://orca.cf.ac.uk/56764/.

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Normal mechanical loading potently induces bone formation mediated by osteocyte effects on osteoblasts. Current in vitro bone models do not reflect these cellular interactions, either focusing on mechanical loading of osteoblasts in monolayers or in 3D and therefore not elucidating the osteocyte-osteoblast interactions that regulate mechanically-induced bone formation. Adenosine, calcium-sensing and glutamate signalling have been shown to influence bone biology, with both adenosine precursors and glutamate having been implicated in mechanotransduction. The aims were to develop a novel in vitro

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Smolina, Margarita. "Breast cancer cell lines grown in a three-dimensional culture model: a step towards tissue-like phenotypes as assessed by FTIR imaging." Doctoral thesis, Universite Libre de Bruxelles, 2018. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/267686.

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Despite the possible common histopathological features at diagnosis, cancer cells present within breast carcinomas are highly heterogeneous in their molecular signatures. This heterogeneity is responsible for disparate clinical behaviors, treatment responses and long-term outcomes in breast cancer patients. Although the few histopathological markers can partially describe the diversity of cells found in tumor tissue sections, the full molecular characterization of individual cancer cells is currently impossible in routine clinical practice. In this respect, Fourier transform infrared (FTIR) mi

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Sorrentino, Rita. "Three dimensional oral mucosa models: development and applications." Doctoral thesis, Università del Piemonte Orientale, 2020. http://hdl.handle.net/11579/114910.

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Animal experimentation has been extensively and for a long time applied in several research fields, but since 2011 it has been substantially limited by the Commission of the European Parliament to ensure people/animals safety and reduce research costs. To respond to these directives, many attempts have been focused on the development and validation of new in vitro 3D systems, bypassing the traditional 2D cell cultures. In this regard, diverse approaches to tissue-engineered bone and oral mucosa have been developed. Despite the promising premises and the cutting-edge results, the used 3D in vit

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9

Cho, Hyung Joon. "Pro-oxidative and Pro-inflammatory Mechanisms of Brain Injury in Experimental Animal and 3D Cell Culture Model Systems." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/73476.

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The pro-oxidative and pro-inflammatory mechanisms have been implicated in various human diseases including neurological and psychiatric disorders. However, there is only limited information available on the etiology in the progression of neurological damage to brain. The emergence of tissue engineering with the growing interest in mechanistic studies of brain injury now raises great opportunities to study complex physiological and pathophysiological process in vitro. Therefore, the prime goals of this study include: (1) Determination of the molecular and cellular mechanisms responsible for bla

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10

Lee, Si Yuen. "Culture of human pluripotent stem cells and neural networks in 3D using an optogenetic approach and a hydrogel model." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:5cecda23-6208-4c0f-a800-d5ddccae24d3.

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Development of optogenetically controllable human neural network models can provide an investigative system that is relevant to the human brain. Conventional cultures of neural networks in two-dimensions (2D) have major limitations of scale. For instance, the soma of neurons in 2D is unrealistically flattened and both axon and dendrite outgrowth is restricted. Using a combination of tissue engineering techniques and the inclusion of optogenetically modified human induced pluripotent stem cell (hiPSC)-derived neural progenitor cells (NPCs), the development of a three-dimensional (3D) human neur

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11

Ståhl, Emmy. "Identification of changes in biomarkers relevant for breast cancer biology occurring in a novel 3D-Biosilk model." Thesis, KTH, Proteinvetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-294248.

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Bröstcancer är den vanligaste formen av cancer som drabbar kvinnor. Det är en heterogen och komplex sjukdom som består av flera undergrupper, var och en med distinkt morfologi och kliniska implikationer [1]. För att modellera och studera cellbiologi, vävnadsmorfologi, molekylära mekanismer och läkemedels effekter används cellkulturer [2]. Idag är tvådimensionella (2D) modeller fortfarande den mest använda metoden för att odla celler in vitro [3]. En nackdel med 2D-modeller är att mikromiljön i dessa modeller inte imiterar in vivo strukturen av tumörer och vävnader, då de saknar tre dimensionel

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Gérémie, Lauriane. "Development of an in-vitro intestinal model featuring peristaltic motion." Thesis, Sorbonne université, 2019. http://accesdistant.sorbonne-universite.fr/login?url=http://theses-intra.upmc.fr/modules/resources/download/theses/2019SORUS118.pdf.

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Le Gut-on-chip fait partie d'un thème de recherche plus générale, appelez Organ-on-chip qui a pour objectif de développer des modèles in-vitro qui récapitulent des caractéristiques essentielles de l'organe d'intérêt. Dans le cas de l'intestin, les Gut-on-chip plateformes ont été principalement développés pour reconstituer soit l'architecture 3D de l'intestin, soit sa dynamique et plus particulièrement le péristaltisme. Durant ma thèse j'ai développé une nouvelle et polyvalente Gut-on-chip, présentant ces deux aspects du micro-environnement intestinale. Cette Peristalsis-on-chip nous a permis d

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Mitterer, Chantal. "The role of inflammation induced by radiation or lipopolysaccharides in the metastatic process in a mouse model of breast cancer." Mémoire, Université de Sherbrooke, 2012. http://hdl.handle.net/11143/6343.

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Mortality from breast cancer is primarily due to metastatic disease, which often appears years after treatment of the primary tumor. Radiation as well as bacterial infection induces inflammation, which by releasing cytokines can be implicated in metastatic processes. Using in vitro and in vivo models, the ability of radiation to awaken dormant lung metastases was assessed as well as the capacity of a bacterial infection to enhance metastatic progression in already proliferating lung metastases. As models, we used the D2.0R (dormant) and D2A1 (proliferative) cell lines, which are derived from s

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Bianchi, Arianna. "Epithelial-mesenchymal interactions in the cornea : development of a novel 3D culture cornea model and progress towards environmental reprogramming of cornea epithelium." Thesis, University of Newcastle upon Tyne, 2015. http://hdl.handle.net/10443/3033.

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The unmet need for corneal epithelial cells for treating human eye diseases makes the cornea important in the cell therapy field. Additionally cornea tissue engineering has become valuable for clinical use, research, and for creating representative models replacing animals for chemical/drug testing. This study initially used qPCR to investigate the expression levels of key markers produced by 2D corneal epithelial cell cultures after wounding in a scratch assay. Then an attempt was made to environmentally reprogram human hair follicle keratinocytes into corneal epithelial cells using limbal ep

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15

Lebeko, Maribanyana Robert. "The use of in vitro 2d co-culture models to determine the optimal keratinocyte: melanocyte ratio to be used in the development of pigmented 3d skin model." Doctoral thesis, University of Cape Town, 2015. http://hdl.handle.net/11427/16564.

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Includes bibliographical references<br>Burn injuries are among the most devastating of all injuries and a major global public health crisis, with fire related burns accounting for approximately 265 000 deaths annually. The African continent, most especially Sub-Saharan Africa, has the second highest mortality rates (15% of global mortality rates). In South Africa, 3.2 % of the total population sustains burn injuries, with 50 % of these cases as children under the age of20 years. Studies have also shown that most of these incidences are prevalent within the age groups of 0-5 years, and account

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Valentin, Loïse. "Développement de modèles de culture en 3D pour l’étude des maladies et des infections hépatiques humaines." Electronic Thesis or Diss., Sorbonne université, 2022. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2022SORUS442.pdf.

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Les infections et maladies hépatiques sont une cause majeure de morbidité et de mortalité. Elles incluent des infections causées par des pathogènes hépatotropiques, comme le parasite Plasmodium, et des maladies d’origine non infectieuse causées notamment par des traitements médicamenteux, telle que la cholestase. L’hépatocyte humain primaire (PHH) est le type cellulaire de choix pour étudier ces infections et pathologies in vitro, mais il est nécessaire de développer de nouveaux modèles de culture plus proches de la physiologie des cellules in vivo et plus pertinents que les modèles 2D. C’est

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17

Rontard, Jessica. "Evaluation expérimentale du risque prion lié aux porteurs asymptomatiques chez l'Homme et le macaque." Thesis, Paris Sciences et Lettres (ComUE), 2018. http://www.theses.fr/2018PSLET008/document.

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La détection de la protéine prion anormale dans les tissus lymphoïdes de patients britanniques suggère qu’après exposition à l’agent de la variante de la maladie de Creutzfeldt-Jakob (vMCJ) plus de 99% des contaminations pourraient demeurer cliniquement silencieuses. Ces données soulignent un risque de transmission secondaire par transfusion sanguine ce qui nous a conduit à une étude expérimentale. En parallèle des formes classiques de vMCJ, nos modèles murins et simiens de retransmission ont mis en evidence des phenotypes atypiques. Ces phénotypes échappent actuellement aux critères de diagno

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18

Mosaad, Eman Mohamed Othman. "Three dimensional prostate cancer model systems." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/118287/1/Eman%20Mohamed%20Othman_Mosaad_Thesis.pdf.

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The prediction of drug efficacy is a major limitation in the cancer research field. This thesis was a step forward in developing an in vitro 3-dimensional prostate cancer model as a potential high throughput drug-screening platform. The merits of using a high throughput microwell platform to efficiently manufacture hundreds of multicellular spheroids were evaluated. The improved Microwell-mesh platform was evaluated as a drug-screening platform. A critical factor was the discovery of the cell-specific bioluminescence assay instability, which was promoter and/or cell line dependent. The first m

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19

Groult, Jessica. "Expansion ex vivo des Cellules Tumorales Circulantes comme modele de pharmacologie predictive des cancers." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS236/document.

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L'émergence des thérapies ciblées dans le traitement des cancers a rendu indispensable la mise au point de marqueurs plus spécifiques et sensibles pour la surveillance des patients. Dès le stade invasif, des cellules tumorales peuvent passer dans le sang où elles constituent les Cellules Tumorales Circulantes (CTC). Les CTC sont accessibles par une simple prise de sang, évitant les biopsies invasives. De plus, elles représentent le seul matériel tumoral résiduel après traitement. C'est la raison pour laquelle les CTC constituent un axe de recherche très actif avec plus de 400 essais cliniques

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Prudon, Nicolas. "Integrative study, from the cell to the animal model, of the development of a cell therapy for Parkinson's disease." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0071.

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Un ensemble d'études précliniques soutient désormais le développement de thérapies de remplacement cellulaire dérivées de cellules souches pluripotentes pour soulager les symptômes moteurs chez les patients parkinsoniens. Le remplacement de la principale population cellulaire dysfonctionnelle au sein de la maladie, les neurones dopaminergiques A9, est l'objectif principal de ces thérapies. Pour y parvenir, la plupart des approches thérapeutiques impliquent la greffe de suspensions cellulaires de progéniteurs dopaminergiques. Cependant, une quantité considérable de cellules meurent pendant le p

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Hammoudi, Taymour Marwan. "3D micropatternable hydrogel systems to examine crosstalk effects between mesenchymal stem cells, osteoblasts, and adipocytes." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/45972.

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Poor skeletal health results from aging and metabolic diseases such as obesity and diabetes and involves impaired homeostatic balance between marrow osteogenesis and adipogenesis. Tissue engineering provides researchers with the ability to generate improved, highly controlled and tailorable in vitro model systems to better understand mechanisms of homeostasis, disease, and healing and regeneration. Model systems that allow assembly of modules of MSCs, osteoblasts, and adipocytes in a number of configurations to engage in signaling crosstalk offer the potential to study integrative physiologica

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Bonda, Ulrich [Verfasser], Carsten [Akademischer Betreuer] Werner, and Dietmar [Gutachter] Hutmacher. "The prostatic tumour stroma : Design and validation of a 3D in vitro angiogenesis co‐culture model / Ulrich Bonda ; Gutachter: Carsten Werner, Dietmar Hutmacher ; Betreuer: Carsten Werner." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-208301.

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Bonda, Ulrich [Verfasser], Carsten [Akademischer Betreuer] [Gutachter] Werner, and Dietmar [Gutachter] Hutmacher. "The prostatic tumour stroma : Design and validation of a 3D in vitro angiogenesis co‐culture model / Ulrich Bonda ; Gutachter: Carsten Werner, Dietmar Hutmacher ; Betreuer: Carsten Werner." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://d-nb.info/1114067938/34.

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Morales, Delphine. "Modèles 3D de mélanome métastatique pour l’évaluation in vitro de l’efficacité de molécules de thérapies ciblées." Thesis, Compiègne, 2019. http://www.theses.fr/2019COMP2498.

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La sensibilité des cellules de mélanomes aux molécules de thérapies ciblées dépend du microenvironnement tumoral (interactions cellule-cellule et cellule-matrice extracellulaire). Les systèmes tridimensionnels (3D) de culture in vitro reflètent mieux l’architecture structurelle native des tissus et sont attrayants pour l’étude des interactions cellulaires. Nous avons développé et comparé plusieurs modèles de mélanome métastatique : les cellules de mélanomes (SK-MEL-28 et SK-MEL-3, mutées BRAF V600E et SK-MEL-2, BRAF sauvages) cultivées en monocouche (2D) et co-cultivées en 3D sur des équivalen

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CERQUENI, GIORGIA. "In vitro strategies and development of bioengineered approaches for studying age-related osteochondral diseases." Doctoral thesis, Università Politecnica delle Marche, 2021. http://hdl.handle.net/11566/292220.

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The purpose of this PhD project is the development of in-vitro culture models to study different aspects of Osteoarthritis (OA), an entire joint degenerative disease that involved articular cartilage, synovium and subchondral bone. The global incidence of knee OA is 203 per 10,000 person-year that grows with the increase of age, reaching a peak between 70-79 years old, thus dragging the peak of prevalence in old age. The main OA disabling symptom is pain that is typically intermittent and weight-bearing (mechanical) and can lead to psychological stress. Contrary to the common description of a

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COSTABILE, FRANCESCA. "Development of an in vitro murine three-dimensional tumor model to study the micro-environment ability to tune cell’s features." Doctoral thesis, Università degli studi di Genova, 2022. http://hdl.handle.net/11567/1079876.

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Background: The tumor microenvironment (TME) is a complex system, shaped by direct interactions among different cell types and by the interactions between cells and extracellular matrix (ECM). Given the emerging importance of the TME in modulating cells’ morphology and function, more sophisticated tumor models incorporating TME features are needed to elucidate cellular, molecular, and immunologic mechanisms of tumor response or resistance. An intensive investigation of in vitro models able to study tumor biology has led to the generation of different three-dimensional (3D) culture methods that

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Latour, Simon. "Rôle des protéines Orai1 et STIM1 dans les lymphomes B non-Hodgkiniens, établissement d'un modèle d'étude en 3D." Thesis, Bordeaux, 2018. http://www.theses.fr/2018BORD0038.

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Les lymphomes B non-Hodgkiniens (LNHB) représentent le type d’hémopathie maligne le plus fréquent. Ces pathologies sont traitées par l’association de chimiothérapies conventionnelles et d’immunothérapies dirigées contre le CD20. Bien qu’efficace, 40% des patients résistent ou rechutent après le traitement. Deux raisons peuvent expliquer ces échecs thérapeutiques : 1) l’absence de cibles thérapeutiques impliquées dans plusieurs processus oncogéniques et 2) l’absence de modèles pré-cliniques de LNHB pertinents pour le test de molécules thérapeutiques et la compréhension de la lymphomagenèse. Le

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Ham, Stephanie Lemmo. "Engineering Tumor Models Using Aqueous Biphasic 3D Culture Microtechnology." University of Akron / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=akron150470381711759.

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Capra, J. (Janne). "Differentiation and malignant transformation of epithelial cells:3D cell culture models." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526218236.

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Abstract The epithelial cells form barriers that compartmentalize the organs. Carcinomas are cancers stemming from epithelial cells and are the most common cancer type. The aim of this study was to understand the differentiation and malignant transformation of epithelial Madin-Darby canine kidney (MDCK) cells and to analyse the electrophysiological parameters which regulate their transport capacity. Emphasis was placed on comparing different culture environments, both in 2D and 3D. First, the effects of drugs or basal extracellular fluid composition on MDCK cell, cyst and lumen volumes were an

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Giudice, Fernanda Salgueiredo. "Análise da expressão das proteínas Akt, NF-kB & Ciclina D1 em linhagens celulares de carcinoma epidermóide de cabeça e pescoço em ambiente tridimensional e câmara de invasão." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/23/23141/tde-29092009-081311/.

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O carcinoma epidermóide representa mais de 90% das neoplasias malignas de cabeça e pescoço, apresentando taxas elevadas de morbi-mortalidade, porém pouco se sabe sobre as vias de sinalização que estão envolvidas na progressão tumoral. Têm sido relatado na literatura, que alguns estímulos podem ativar a holoenzima PI3K que, por sua vez, desencadeia um processo que induz a fosforilação da proteína Akt (pAkt) que leva a ativação e a translocação do NF-B do citoplasma para o núcleo, onde ocorre a transcrição de genes envolvidos na proliferação e invasão celular. Assim, esse estudo analisou, atravé

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Teixeira, Marta Carolina Jesus. "Patient-derived explant cultures for cancer modelling." Master's thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Quimica e Biológica António Xavier, 2018. http://hdl.handle.net/10362/130073.

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"Cancer remains one of the deadliest diseases in the world. The process of drug discovery to find new drugs for oncology treatment takes more than a decade of research and it is associated with high attrition rates during clinical trials. One of the most common reasons claimed for the poor efficiency of new developed drugs is the lack of physiological relevance of the in vitro models used to select the novel compounds and to evaluate their potency. The role of tumor microenvironment on tumor progression and drug sensitivity is being increasingly studied a

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ES, SEBAR LEILA. "Metrology for Cultural Heritage: multispectral 3D models by photogrammetry." Doctoral thesis, Politecnico di Torino, 2022. http://hdl.handle.net/11583/2959961.

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Held, Christoph. "Creating 3D models of cultural heritage sites with terrestrial laser scanning and 3D imaging." Master's thesis, University of Cape Town, 2012. http://hdl.handle.net/11427/12076.

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Includes abstract.<br>Includes bibliographical references.<br>The advent of terrestrial laser-scanners made the digital preservation of cultural heritage sites an affordable technique to produce accurate and detailed 3D-computermodel representations for any kind of 3D-objects, such as buildings, infrastructure, and even entire landscapes. However, one of the key issues with this technique is the large amount of recorded points; a problem which was even more intensified by the recent advances in laser-scanning technology, which increased the data acquisition rate from 25 thousand to 1 million p

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Kim, Leejin. "Analysis and Construction of Engaging Facial Forms and Expressions: Interdisciplinary Approaches from Art, Anatomy, Engineering, Cultural Studies, and Psychology." VCU Scholars Compass, 2013. http://scholarscompass.vcu.edu/etd/567.

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The topic of this dissertation is the anatomical, psychological, and cultural examination of a human face in order to effectively construct an anatomy-driven 3D virtual face customization and action model. In order to gain a broad perspective of all aspects of a face, theories and methodology from the fields of art, engineering, anatomy, psychology, and cultural studies have been analyzed and implemented. The computer generated facial customization and action model were designed based on the collected data. Using this customization system, culturally-specific attractive face in Korean popular

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Schmittlein, Carina [Verfasser], and Joachim [Akademischer Betreuer] Wegener. "Sensors and Actuators for 2D and 3D Cell Culture Models based on Oxygen Sensitive Culture Substrates / Carina Schmittlein ; Betreuer: Joachim Wegener." Regensburg : Universitätsbibliothek Regensburg, 2018. http://d-nb.info/1170955738/34.

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CAMPIONI, GLORIA. "Monolayers and three-dimensional cultures to investigate metabolic reprogramming in breast and bladder cancer." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2022. http://hdl.handle.net/10281/375413.

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La riprogrammazione metabolica è stata osservata in molti tipi di cancro ed è considerata un tratto distintivi di questa malattia eterogenea multifattoriale. Comprendere i meccanismi che portano al riarrangiamento metabolico e come queste attività promuovono l’attivazione di proprietà maligne nel cancro può aiutare a sfruttare le alterazioni metaboliche a beneficio terapeutico. Nei tumori solidi le cellule tumorali interagiscono con il complesso habitat del microambiente tumorale (TME) che può modulare il metabolismo delle cellule tumorali e la loro sensibilità o resistenza al trattamento farm

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Kós, José Ripper. "Urban spaces shaped by past cultures : historical representation through electronic 3D models and databases." Thesis, University of Strathclyde, 2003. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=21518.

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Digital tools have been increasingly used, in the last decades, for the study and representation of the city history. As the available instruments develop and the researchersb ecome more familiar with them, their use turns out to be more effective and provides richer results. This study aims to explore the use of information technology, particularly 3D models, for the city history research. When this study was elaborated, few initiatives effectivelly applied those new tools to convey the history of the city. A smaller number of published scientific enterprises investigated that operation. Ther

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FUCARINO, Alberto Giuseppe. "APPLICATION OF NOVEL 3D CULTURE MODELS OF HUMAN MUCOSAE TO STUDY THE EFFECTS OF ENVIRONMENTAL FACTORS ON NON-COMMUNICABLE DISEASES." Doctoral thesis, Università degli Studi di Palermo, 2014. http://hdl.handle.net/10447/90906.

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BOSIO, ALESSIA GRAZIANA. "IDENTIFICATION OF NOVEL BIGUANIDE BASEDCOMPOUNDS AS ANTI TUMOR AGENTS FOR GLIOBLASTOMA: PHARMACOLOGICAL ASSESSMENT IN 3D CULTURE MODELS." Doctoral thesis, Università degli studi di Genova, 2022. http://hdl.handle.net/11567/1090330.

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Glioblastoma (GBM) is the most common malignant and aggressive adult brain tumour characterized by its clinical behaviour, with high growth rate, diffuse invasiveness, and low response to therapies. Despite of multimodal treatment, which consists in extensive surgery followed by radiotherapy and chemotherapy (temozolomide, TMZ) the mean life expectancy for patients with GBM is less than 2 years. Although remarkable research efforts have been done in the last decades, no significant improvement in patient survival have been obtained from 2005, also due to the lack of appropriate models to study

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Hörr, Christian, Elisabeth Lindinger, and Guido Brunnett. "Considerations on Technical Sketch Generation from 3D Scanned Cultural Heritage." Universitätsbibliothek Chemnitz, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-200901463.

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Drawing sketches is certainly one of the most important but at the same time elaborate parts of archaeological work. Currently, 3D scanning technology is affording a number of new applications, and only one of them is using virtual copies instead of the originals as the basis for documentation. Our major contribution are methods for automatically generating stylized images from 3D models. These are not only intuitive and easy to read but also more objective and accurate than traditional drawings. Besides some other useful tools we show several examples from our daily work proving that the syst

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Barnes, Devon. "In vitro bioengineering applications of melt electrowritten and hydrogel composite scaffolds." Thesis, Queensland University of Technology, 2021. https://eprints.qut.edu.au/212352/1/Devon_Barnes_Thesis.pdf.

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Two-dimensional cell cultures provide an inaccurate representation of how cells develop and are affected by disease and injury. Scaffold-based tissue engineering techniques that combine novel biomaterials and printing methods could assist in the design of more physiologically relevant, three-dimensional experimental tissue models. This thesis investigated the application of carbohydrate glass as a sacrificial material toward producing perfusable hydrogel devices using melt electrowriting, the development and optimisation of a three-dimensional bioengineered bone marrow microenvironment, and a

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DI, LISA DONATELLA. "Biopolymeric microbeads as a 3D scaffold for soft tissue engineering." Doctoral thesis, Università degli studi di Genova, 2020. http://hdl.handle.net/11567/1005298.

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The increase of different types of cell cultures, which can be used for the in vitro studies of physiological and/or pathological processes, has introduced the need to improve culture techniques through the use of materials and culture media that promote growth, recreating a cellular micro-environment that can be asserted in in vivo condition. Therefore, it is important to design and develop new biologically sustainable methods, such as to contribute to the “closer-to-in vivo” condition. In particular, the design of a 3D in vitro model of neuronal culture is an important step to better unders

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Rambani, Komal. "Thick brain slice cultures and a custom-fabricated multiphoton imaging system: progress towards development of a 3D hybrot model." Thesis, Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/22702.

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Development of a three dimensional (3D) HYBROT model with targeted in vivo like intact cellular circuitry in thick brain slices for multi-site stimulation and recording will provide a useful in vitro model to study neuronal dynamics at network level. In order to make this in vitro model feasible, we need to develop several associated technologies. These technologies include development of a thick organotypic brain slice culturing method, a three dimensional (3D) micro-fluidic multielectrode Neural Interface system (ÂµNIS) and the associated electronic interfaces for stimulation and recording

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Picone, Giovanna <1992&gt. "Study of Bone Biomineralization in 2D and 3D Cultures of a Human Osteosarcoma Cell Line As Osteoblast-like Model." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2022. http://amsdottorato.unibo.it/10235/1/Giovanna_Picone_tesi.pdf.

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This PhD project focuses on the study of the early stages of bone biomineralization in 2D and 3D cultures of osteoblast-like SaOS-2 osteosarcoma cells, exposed to an osteogenic cocktail. The efficacy of osteogenic treatment was assessed on 2D cell cultures after 7 days. A large calcium minerals production, an overexpression of osteogenic markers and of alkaline phosphatase activity occurred in treated samples. TEM microscopy and cryo-XANES micro-spectroscopy were performed for localizing and characterizing Ca-depositions. These techniques revealed a different localization and chemical composit

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Dean, Zachary S. "Collective Migration Models: Dynamic Monitoring of Leader Cells in Migratory/Invasive Disease Processes." Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/560817.

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Leader cells are a fundamental biological process that have only been investigated since the early 2000s. These cells have often been observed emerging at the edge of an artificial wound in 2D epithelial cell collective invasion, created with either a mechanical scrape from a pipette tip or from the removal of a plastic, physical blocker. During migration, the moving cells maintain cell-cell contacts, an important quality of collective migration; the leader cells originate from either the first or the second row, they increase in size compared to other cells, and they establish ruffled lamel

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Ferreira, Luís Pedro Correia Pinto. "Development of multicelular 3D cancer testing platforms for evaluation of new anti-cancer therapies." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22713.

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Mestrado em Bioquímica Clínica<br>O cancro do pulmão (CP) é um dos cancros mais diagnosticados a nível mundial e também um dos mais mortíferos. Atualmente, as terapias administradas a nível clínico para o tratamento do CP são ainda extremamente ineficazes e limitadas no que diz respeito ao aumento da taxa de sobrevivência dos pacientes oncológicos. Esta realidade demonstra a necessidade de investigar ativamente novas terapias para o tratamento desta neoplasia. No entanto a validação pré-clínica de terapias inovadoras para o CP tem-se revelado extremamente difícil devido à inexistência de plat

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UREÑA, MARTÍN Carlos. "Study of Caveolae Mechanotransduction Under 3D Compressive Stresses : Comparative Analysis of 2 Models Mimicking Structural and Mechanical Tumor Characteristics." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS525.

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La mécanique et le stress compressif jouent un rôle important dans la progression tumorale. Récemment, plusieurs approches ont été développées pour tester le stress en compression dans des modèles 3D in vitro. Dans le présent travail, nous montrons d’abord la pertinence de la compression dans l’organisation des fibroblastes associés au cancer (CAF), en enveloppant les cellules cancéreuses lors d’une compression isotrope 3D dans des capsules d’alginate creux. Dans ce système, les CAF couvrent les cellules cancéreuses en présence de compression selon un processus impliquant vraisemblablement une

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Bertacchi, Gianna <1990&gt. "Guidelines for the Management of Cultural Heritage using 3D models for the insertion of heterogeneous data." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2022. http://amsdottorato.unibo.it/10046/1/bertacchi_gianna_tesi.pdf.

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The Management of Cultural Heritage (MCH) is a very complex operation aimed at protecting the physical integrity of CH assets, while promoting their historical value and development of tourism industry. In recent years, the use of digital technologies has become an essential part of the MCH delicate process, but the use of 3D models is still limited to few academic research to date. Furthermore, very few supra-national standard guidelines regulating their use are available to date and the operator who decides to use a 3D model as a basis for management is faced with the scarcity and fragmentat

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Eggert, Sebastian. "Design and development of an open-source technology platform for automated manufacturing and screening of 3D cell culture models: A systems engineering approach." Thesis, Queensland University of Technology, 2021. https://eprints.qut.edu.au/212977/1/Sebastian_Eggert_Thesis.pdf.

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This PhD thesis developed, engineered and applied a novel open-source technology platform for automated manufacturing and screening of 3D cell culture models. The modular approach not only enabled agile and inexpensive development of automated solutions, but also facilitated reproducible, scalable, and efficient workflows. The results indicated that such automated solutions are a much-needed tool for in vitro research applications as well as pharmaceutical studies to increase reproducibility and throughput, resulting in time, labour, and consequently cost reductions.

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Amaral, Jonatas Bussador do. "Células MCF-7 como modelo 3D no estudo de câncer de mama humano." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-21072011-134443/.

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O diferencial da cultura de células em 3-dimensões é permitir que as células explorem as 3-dimensões do espaço, aumentando assim as interações com o ambiente e entre as células. Em estudos relacionados à biologia do câncer de mama, vem ganhando espaço a utilização de esferóides para estudos que visam à compreensão da morfogênese do espaço luminal. Neste trabalho foi mostrado que as células MCF-7 reorganizam-se em estruturas tubulares e acinares. Em ambas as situações, a formação do lúmen veio acompanhada pelo estabelecimento de uma camada de células polarizadas, arranjo este muito semelhante a

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