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Relevant bibliographies by topics / 3D co-cultures

Academic literature on the topic '3D co-cultures'

Author: Grafiati

Published: 10 December 2022

Last updated: 28 January 2023

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Journal articles on the topic "3D co-cultures"

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Thanapirom, Kessarin, Elisabetta Caon, Margarita Papatheodoridi, Luca Frenguelli, Walid Al-Akkad, Zhang Zhenzhen, Maria Giovanna Vilia, Massimo Pinzani, Giuseppe Mazza, and Krista Rombouts. "Optimization and Validation of a Novel Three-Dimensional Co-Culture System in Decellularized Human Liver Scaffold for the Study of Liver Fibrosis and Cancer." Cancers 13, no. 19 (September 30, 2021): 4936. http://dx.doi.org/10.3390/cancers13194936.

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The introduction of new preclinical models for in vitro drug discovery and testing based on 3D tissue-specific extracellular matrix (ECM) is very much awaited. This study was aimed at developing and validating a co-culture model using decellularized human liver 3D ECM scaffolds as a platform for anti-fibrotic and anti-cancer drug testing. Decellularized 3D scaffolds obtained from healthy and cirrhotic human livers were bioengineered with LX2 and HEPG2 as single and co-cultures for up to 13 days and validated as a new drug-testing platform. Pro-fibrogenic markers and cancer phenotypic gene/protein expression and secretion were differently affected when single and co-cultures were exposed to TGF-β1 with specific ECM-dependent effects. The anti-fibrotic efficacy of Sorafenib significantly reduced TGF-β1-induced pro-fibrogenic effects, which coincided with a downregulation of STAT3 phosphorylation. The anti-cancer efficacy of Regorafenib was significantly reduced in 3D bioengineered cells when compared to 2D cultures and dose-dependently associated with cell apoptosis by cleaved PARP-1 activation and P-STAT3 inhibition. Regorafenib reversed TGF-β1-induced P-STAT3 and SHP-1 through induction of epithelial mesenchymal marker E-cadherin and downregulation of vimentin protein expression in both co-cultures engrafting healthy and cirrhotic 3D scaffolds. In their complex, the results of the study suggest that this newly proposed 3D co-culture platform is able to reproduce the natural physio-pathological microenvironment and could be employed for anti-fibrotic and anti-HCC drug screening.

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Truzzi, Francesca, Camilla Tibaldi, Anne Whittaker, Silvia Dilloo, Enzo Spisni, and Giovanni Dinelli. "Pro-Inflammatory Effect of Gliadins and Glutenins Extracted from Different Wheat Cultivars on an In Vitro 3D Intestinal Epithelium Model." International Journal of Molecular Sciences 22, no. 1 (December 26, 2020): 172. http://dx.doi.org/10.3390/ijms22010172.

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There is a need to assess the relationship between improved rheological properties and the immunogenic potential of wheat proteins. The present study aimed to investigate the in vitro effects of total protein extracts from three modern and two landrace Triticum aestivum commercial flour mixes, with significant differences in gluten strength (GS), on cell lines. Cytotoxicity and innate immune responses induced by wheat proteins were investigated using Caco-2 monocultures, two dimensional (2D) Caco-2/U937 co-cultures, and three dimensional (3D) co-cultures simulating the intestinal mucosa with Caco-2 epithelial cells situated above an extra-cellular matrix containing U937 monocytes and L929 fibroblasts. Modern wheat proteins, with increased GS, significantly reduced Caco-2 cell proliferation and vitality in monoculture and 2D co-cultures than landrace proteins. Modern wheat proteins also augmented Caco-2 monolayer disruption and tight junction protein, occludin, redistribution in 3D co-cultures. Release of interleukin-8 into the cell medium and increased U937 monocyte migration in both 2D and 3D co-cultures were similarly apparent. Immuno-activation of migrating U937 cells was evidenced from cluster of differentiation 14 (CD14) staining and CD11b-related differentiation into macrophages. The modern wheat proteins, with gluten polymorphism relatedness and increased GS, were shown to be more cytotoxic and immunogenic than the landrace wheat proteins.

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Smithmyer, Megan E., Christopher C. Deng, Samantha E. Cassel, Paige J. LeValley, Brent S. Sumerlin, and April M. Kloxin. "Self-Healing Boronic Acid-Based Hydrogels for 3D Co-cultures." ACS Macro Letters 7, no. 9 (August 31, 2018): 1105–10. http://dx.doi.org/10.1021/acsmacrolett.8b00462.

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Soriano, Luis, Tehreem Khalid, Fergal J. O'Brien, Cian O'Leary, and Sally-Ann Cryan. "A Tissue-Engineered Tracheobronchial In Vitro Co-Culture Model for Determining Epithelial Toxicological and Inflammatory Responses." Biomedicines 9, no. 6 (June 2, 2021): 631. http://dx.doi.org/10.3390/biomedicines9060631.

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Translation of novel inhalable therapies for respiratory diseases is hampered due to the lack of in vitro cell models that reflect the complexity of native tissue, resulting in many novel drugs and formulations failing to progress beyond preclinical assessments. The development of physiologically-representative tracheobronchial tissue analogues has the potential to improve the translation of new treatments by more accurately reflecting in vivo respiratory pharmacological and toxicological responses. Herein, advanced tissue-engineered collagen hyaluronic acid bilayered scaffolds (CHyA-B) previously developed within our group were used to evaluate bacterial and drug-induced toxicity and inflammation for the first time. Calu-3 bronchial epithelial cells and Wi38 lung fibroblasts were grown on either CHyA-B scaffolds (3D) or Transwell® inserts (2D) under air liquid interface (ALI) conditions. Toxicological and inflammatory responses from epithelial monocultures and co-cultures grown in 2D or 3D were compared, using lipopolysaccharide (LPS) and bleomycin challenges to induce bacterial and drug responses in vitro. The 3D in vitro model exhibited significant epithelial barrier formation that was maintained upon introduction of co-culture conditions. Barrier integrity showed differential recovery in CHyA-B and Transwell® epithelial cultures. Basolateral secretion of pro-inflammatory cytokines to bacterial challenge was found to be higher from cells grown in 3D compared to 2D. In addition, higher cytotoxicity and increased basolateral levels of cytokines were detected when epithelial cultures grown in 3D were challenged with bleomycin. CHyA-B scaffolds support the growth and differentiation of bronchial epithelial cells in a 3D co-culture model with different transepithelial resistance in comparison to the same co-cultures grown on Transwell® inserts. Epithelial cultures in an extracellular matrix like environment show distinct responses in cytokine release and metabolic activity compared to 2D polarised models, which better mimic in vivo response to toxic and inflammatory stimuli offering an innovative in vitro platform for respiratory drug development.

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Ruoß, Marc, Vanessa Kieber, Silas Rebholz, Caren Linnemann, Helen Rinderknecht, Victor Häussling, Marina Häcker, Leon H. H. Olde Damink, Sabrina Ehnert, and Andreas K. Nussler. "Cell-Type-Specific Quantification of a Scaffold-Based 3D Liver Co-Culture." Methods and Protocols 3, no. 1 (December 23, 2019): 1. http://dx.doi.org/10.3390/mps3010001.

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In order to increase the metabolic activity of human hepatocytes and liver cancer cell lines, many approaches have been reported in recent years. The metabolic activity could be increased mainly by cultivating the cells in 3D systems or co-cultures (with other cell lines). However, if the system becomes more complex, it gets more difficult to quantify the number of cells (e.g., on a 3D matrix). Until now, it has been impossible to quantify different cell types individually in 3D co-culture systems. Therefore, we developed a PCR-based method that allows the quantification of HepG2 cells and 3T3-J2 cells separately in a 3D scaffold culture. Moreover, our results show that this method allows better comparability between 2D and 3D cultures in comparison to the often-used approaches based on metabolic activity measurements, such as the conversion of resazurin.

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Rausch, Magdalena, Léa Blanc, Olga De Souza Silva, Olivier Dormond, Arjan W. Griffioen, and Patrycja Nowak-Sliwinska. "Characterization of Renal Cell Carcinoma Heterotypic 3D Co-Cultures with Immune Cell Subsets." Cancers 13, no. 11 (May 22, 2021): 2551. http://dx.doi.org/10.3390/cancers13112551.

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Two-dimensional cell culture-based platforms are easy and reproducible, however, they do not resemble the heterotypic cell-cell interactions or the complex tumor microenvironment. These parameters influence the treatment response and the cancer cell fate. Platforms to study the efficacy of anti-cancer treatments and their impact on the tumor microenvironment are currently being developed. In this study, we established robust, reproducible, and easy-to-use short-term spheroid cultures to mimic clear cell renal cell carcinoma (ccRCC). These 3D co-cultures included human endothelial cells, fibroblasts, immune cell subsets, and ccRCC cell lines, both parental and sunitinib-resistant. During spheroid formation, cells induce the production and secretion of the extracellular matrix. We monitored immune cell infiltration, surface protein expression, and the response to a treatment showing that the immune cells infiltrated the spheroid co-cultures within 6 h. Treatment with an optimized drug combination or the small molecule-based targeted drug sunitinib increased immune cell infiltration significantly. Assessing the therapeutic potential of this drug combination in this platform, we revealed that the expression of PD-L1 increased in 3D co-cultures. The cost- and time-effective establishment of our 3D co-culture model and its application as a pre-clinical drug screening platform can facilitate the treatment validation and clinical translation.

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Martinez-Pacheco, Sarai, and Lorraine O’Driscoll. "Pre-Clinical In Vitro Models Used in Cancer Research: Results of a Worldwide Survey." Cancers 13, no. 23 (November 30, 2021): 6033. http://dx.doi.org/10.3390/cancers13236033.

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To develop and subsequently get cancer researchers to use organotypic three-dimensional (3D) models that can recapitulate the complexity of human in vivo tumors in an in vitro setting, it is important to establish what in vitro model(s) researchers are currently using and the reasons why. Thus, we developed a survey on this topic, obtained ethics approval, and circulated it throughout the world. The survey was completed by 101 researchers, across all career stages, in academia, clinical or industry settings. It included 40 questions, many with multiple options. Respondents reported on their field of cancer research; type of cancers studied; use of two-dimensional (2D)/monolayer, 2.5D and/or 3D cultures; if using co-cultures, the cell types(s) they co-culture; if using 3D cultures, whether these involve culturing the cells in a particular way to generate spheroids, or if they use additional supports/scaffolds; techniques used to analyze the 2D/2.5D/3D; and their downstream applications. Most researchers (>66%) only use 2D cultures, mainly due to lack of experience and costs. Despite most cancer researchers currently not using the 3D format, >80% recognize their importance and would like to progress to using 3D models. This suggests an urgent need to standardize reliable, robust, reproducible methods for establishing cost-effective 3D cell culture models and their subsequent characterization.

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Meretoja, Ville V., Rebecca L. Dahlin, Sarah Wright, F. Kurtis Kasper, and Antonios G. Mikos. "Articular Chondrocyte Redifferentiation in 3D Co-cultures with Mesenchymal Stem Cells." Tissue Engineering Part C: Methods 20, no. 6 (June 2014): 514–23. http://dx.doi.org/10.1089/ten.tec.2013.0532.

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Nuñez-Olvera, Stephanie I., Lorena Aguilar-Arnal, Mireya Cisneros-Villanueva, Alfredo Hidalgo-Miranda, Laurence A. Marchat, Yarely M. Salinas-Vera, Rosalio Ramos-Payán, et al. "Breast Cancer Cells Reprogram the Oncogenic lncRNAs/mRNAs Coexpression Networks in Three-Dimensional Microenvironment." Cells 11, no. 21 (November 1, 2022): 3458. http://dx.doi.org/10.3390/cells11213458.

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Organotypic three-dimensional (3D) cell cultures more accurately mimic the characteristics of solid tumors in vivo in comparison with traditional two-dimensional (2D) monolayer cell models. Currently, studies on the regulation of long non-coding RNAs (lncRNAs) have not been explored in breast cancer cells cultured in 3D microenvironments. In the present research, we studied the expression and potential roles of lncRNAs in estrogen receptor-positive luminal B subtype BT-474 breast cancer cells grown over extracellular matrix proteins-enriched 3D cultures. Global expression profiling using DNA microarrays identifies 290 upregulated and 183 downregulated lncRNAs in 3D cultures relative to 2D condition. Using a co-expression analysis approach of lncRNAs and mRNAs pairs expressed in the same experimental conditions, we identify hundreds of regulatory axes modulating genes involved in cancer hallmarks, such as responses to estrogens, cell proliferation, hypoxia, apical junctions, and resistance to endocrine therapy. In addition, we identified 102 lncRNAs/mRNA correlations in 3D cultures, which were similar to those reported in TCGA datasets obtained from luminal B breast cancer patients. Interestingly, we also found a set of mRNAs transcripts co-expressed with LINC00847 and CTD-2566J3.1 lncRNAs, which were predictors of pathologic complete response and overall survival. Finally, both LINC00847 and CTD -2566J3.1 were co-expressed with essential genes for cancer genetic dependencies, such as FOXA1 y GINS2. Our experimental and predictive findings show that co-expressed lncRNAs/mRNAs pairs exhibit a high degree of similarity with those found in luminal B breast cancer patients, suggesting that they could be adequate pre-clinical tools to identify not only biomarkers related to endocrine therapy response and PCR, but to understand the biological behavior of cancer cells in 3D microenvironments.

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Kaushik, Garima, Bhavna Verma, and Amy Wesa. "88 Development of a 3D organoid autologous TIL co-culture platform for high throughput immuno-oncology studies." Journal for ImmunoTherapy of Cancer 8, Suppl 3 (November 2020): A98. http://dx.doi.org/10.1136/jitc-2020-sitc2020.0088.

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BackgroundThe preclinical screening of immune-modulatory therapies suffers from the absence of models that recapitulate in vivo heterogeneous tumor microenvironment (TME). 3D tumor organoid cultures provide a model that closely mimics in situ tumor architecture and is being aggressively used to evaluate therapeutic efficacy ex vivo. A vastly heterogenous TME impacts patient treatment response, and there is a dearth of human tumor models (2D or 3D), that mimic in vivo diversity of TME, including infiltrating immune populations. 3D organoid cultures typically contain neoplastic epithelium; however, they fall short in representing tumor to tumor-infiltrating lymphocytes (TILs) interactions, limiting their ability to generate a clinically relevant response to immunotherapeutics. Addition of immune cells from unrelated donors to organoids can simulate that microenvironment but is complicated by T cell alloreactivity. Here we describe 3D patient-derived xenograft organoid (PDXO) co-cultures with matching autologous human TILs to recapitulate the tumor-specific immune response, leveraging confocal high content analysis and luminex multiplex assays. This platform allows the evaluation and high throughput screening of novel immune targeting agents to determine impacts on patient-derived T cell function, T cell infiltration, and tumor cytotoxicity.MethodsSurgical resections from patients were used to generate patient-derived xenografts and tumor-infiltrating lymphocytes in parallel. PDX were resected and digested to establish PDXO. TILs and organoids from the same patient were fluorescent labeled and cultured together for four days to evaluate tumor infiltration and drug cytotoxicity in 3D cultures. CellInsight CX7 high content imaging platform was used to trace TILs and cancer cells and evaluate T cell infiltration and tumor cell killing in the presence and absence of immuno-modulatory therapies.ResultsPDXO were established to mimic in vivo tumor biology. Tumor-specific TILs were successfully expanded and characterized by flow cytometry. Co-culture resulted in TIL infiltration in organoids from day one in culture and increased over four days. Cytotoxicity and TIL infiltration were quantified using fluorescent dyes via high throughput imaging platform. Significantly enhanced TIL infiltration was observed in autologous co-cultures compared to non-autologous co-cultures. The established unique autologous PDXO immune organoid co-cultures could be used as an improved simulation of the modulatory activity of therapeutic agents in patient-specific T cells against their own tumors.ConclusionsPatient autologous TILs – PDXO co-culture platform is an advanced model for evaluating IO therapeutics with the tumor-specific immune microenvironment. The platform provides an opportunity for precision medicine and high throughput drug screening of immuno-modulatory therapies.Ethics ApprovalThe study was approved by Champions Oncology’s Institutional Animal Care and Use Committee (IACUC).

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Dissertations / Theses on the topic "3D co-cultures"

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Freyer, Nora [Verfasser]. "Optimizing culture conditions for hepatic differentiation of human induced pluripotent stem cells : from 3D culture systems to co-cultures / Nora Freyer." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2018. http://d-nb.info/1160514968/34.

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Gariboldi, Maria Isabella. "Effect of calcium phosphate ceramic architectural features on the self-assembly of microvessels in vitro." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/283005.

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One of the greatest obstacles to clinical translation of bone tissue engineering is the inability to effectively and efficiently vascularise scaffolds. This limits the size of defects that can be repaired, as blood perfusion is necessary to provide nutrient and waste exchange to tissue at the core of scaffolds. The goal of this work was to systematically explore whether architecture, at a scale of hundreds of microns, can be used to direct the growth of microvessels into the core of scaffolds. A pipeline was developed for the production of hydroxyapatite surfaces with controlled architecture. Three batches of hydroxyapatite were used with two different particle morphologies and size distributions. On sintering, one batch remained phase pure and the other two batches were biphasic mixtures of α-tricalcium phosphate (α-TCP) and hydroxyapatite. Sample production methods based on slip casting of a hydroxyapatite-gelatin slurry were explored. The most successful of these involved the use of curable silicone to produce moulds of high-resolution, three dimensional (3D) printed parts with the desired design. Parts were dried and sintered to produce patterned surfaces with higher resolution than obtainable through conventional 3D printing techniques. Given the difficulties associated with the structural reproducibility of concave pores architectures in 3D reported in the literature, in this work, a 2.5D model has been developed that varies architectural parameters in a controlled manner. Six contrasting architectures consisting of semi-circular ridges and grooves were produced. Grooves and ridges were designed to have widths of 330 μm and 660 μm, with periodicities, respectively, of 1240 μm and 630 μm. Groove depth was varied between 150 μm and 585 μm. Co-cultures of endothelial cells and osteoblasts were optimised and used to grow microcapillary-like structures (referred to as "microvessels") on substrates. Literature shows that these precursors to microcapillaries contain lumina and can produce functional vasculature, demonstrating their clinical promise. The effects of the composition and surface texture of grooved samples on microvessel formation were studied. It was found that surface microtopography and phase purity (α-TCP content) did not affect microvessel formation. However, hydroxyapatite architecture was found to significantly affect microvessel location and orientation. Microvessels were found to form predominantly in grooves or between convexities. Two metrics - the degree of alignment (DOA) and the degree of containment (DOC) - were developed to measure the alignment of endothelial cell structures and their localisation in grooves. For all patterned samples, the CD31 (an endothelial cell marker) signal was at least 2.5 times higher along grooves versus perpendicular to grooves. In addition, the average signal was at least two times higher within grooves than outside grooves for all samples. Small deep grooves had the highest DOA and DOC (6.13 and 4.05 respectively), and individual, highly aligned microvessels were formed. An image analysis method that compares sample X-ray microtomography sections to original designs to quantify architectural distortion was developed. This method will serve as a useful tool for improvements to architectural control for future studies. This body of work shows the crucial influence of architecture on microvessel self-assembly at the hundreds of micron scale. It also highlights that microvessel formation has a relatively low sensitivity to phase composition and microtopography. These findings have important implications for the design of porous scaffolds and the refinement of fabrication technologies. While important results were shown for six preliminary architectures, this work represents a toolkit that can be applied to screen any 2.5D architecture for its angiogenic potential. This work has laid the foundations that will allow elucidating the precise correspondence between architecture and microvessel organisation, ultimately enabling the "engineering" of microvasculature by tuning local scaffold design to achieve desirable microvessel properties.

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Chen, Xiang Lin, and 陳翔麟. "Through paracrine stimulation effect on chondrogenesis between human mesenchymal stem cells and chondrocytes in 3D co-cultures." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/12933394957866871172.

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碩士
國立清華大學
化學工程學系
103
While cartilage has very limited self-regeneration capacity, and the source of chondrocytes is limited harvesting, mesenchymal stem cells (MSCs) provide an attractive substitute of cartilage tissue engineering as the precursor or progenitor cells that possess the ability to differentiate into functional chondrocytes without ethical and moral concerns. In order to promote re-differentiation without dedifferentiation of chondrocytes as well as to prevent the cell hypertrophy while MSCs tend to undergo chondrogenic differentiation, co-culture strategies were used in this study, and we establish a co-culture system (monolayer and 3D non-contact) of human (rabbit) chondrocytes and human MSCs without any other growth factors to observe the paracrine stimulation effect between these two cells. Therefore, we explore whether MSCs could be promoted chondrogenesis or chondrocytes could be stimulated toward normal functional phenotype (re-differentiation) by so-called trophic effect of MSCs. After the CM-culture (chondrocyte-cultured medium for MSCculturing) and the 3D non-contact co-culture, MSCs gradually loss the chondrogenic phenotype along with culture days while the more stable chondrogenic phenotype of chondrocytes. But the exchange of soluble factors between these two cells makes MSCs produce cartilage-like ECM (GAGs). On the other hand, the direct co-culture shows the gradual enhancement of chondrogenic phenotype oh MSCs.

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Book chapters on the topic "3D co-cultures"

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Unger, Ronald E., Sven Halstenberg, Anne Sartoris, and C. James Kirkpatrick. "Human Endothelial and Osteoblast Co-cultures on 3D Biomaterials." In Methods in Molecular Biology, 229–41. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-984-0_15.

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Purtscher, Michaela, Mario Rothbauer, Wolfgang Holnthoner, Heinz Redl, and Peter Ertl. "Establishment of Vascular Networks in Biochips Using Co-cultures of Adipose Derived Stem Cells and Endothelial Cells in a 3D Fibrin Matrix." In IFMBE Proceedings, 313–17. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-11128-5_78.

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Bowers, Stephanie L. K., Chun-Xia Meng, Matthew T. Davis, and George E. Davis. "Investigating Human Vascular Tube Morphogenesis and Maturation Using Endothelial Cell-Pericyte Co-cultures and a Doxycycline-Inducible Genetic System in 3D Extracellular Matrices." In Methods in Molecular Biology, 171–89. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1164-6_12.

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GIRÓN BASTIDAS, JULIANA, NATASHA MAURMANN, LUIZA SILVA DE OLIVEIRA, and PATRICIA PRANKE. "IN VIVO EVALUATION OF A BILAYER SCAFFOLD FROM PLGA/FIBRIN AND FIBRIN HYDROGEL FOR SKIN REGENERATION." In Proceedings of the 2nd International Digital Congress on 3D Biofabrication and Bioprinting (3DBB) - Biofabrication, Bioprinting, Additive Manufacturing applied to health. Editora Realize, 2022. http://dx.doi.org/10.46943/ii.3dbb.2022.01.016.

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BECAUSE THE INCIDENCE OF SKIN WOUNDS REQUIRING CLINICAL TREATMENT REPRESENTS A PUBLIC HEALTH PROBLEM WORLDWIDE, THE PRESENT WORK AIMS TO DEVELOP A BILAYER SCAFFOLD OF POLY (LACTIDE- CO- GLYCOLIDE) (PLGA)/FIBRIN ELECTROSPUN MEMBRANE AND FIBRIN HYDROGEL LAYER TO BE TESTED IN VIVO AS SKIN SUBSTITUTES. PRIMARY CULTURES OF FIBROBLASTS AND KERATINOCYTES WERE ISOLATED FROM ISOGENIC WISTAR KYOTO RATS (WKY). FIBROBLASTS WERE CULTIVATED IN THE FIBRIN HYDROGEL AND KERATINOCYTES ON THE ELECTROSPUN LAYER TO GENERATE A SKIN SUBSTITUTE USING AN AIR/LIQUID SYSTEM. SCAFFOLDS WERE TESTED IN A FULL-THICKNESS WOUND MODEL IN WKY RATS OF 3 MONTHS. THREE GROUPS WERE ANALYZED MACROSCOPICALLY AND MICROSCOPICALLY: 1 (BILAYER SCAFFOLD WITHOUT CELLS), 2 (HETEROTYPIC SKIN SUBSTITUTES), 3 (NEGATIVE CONTROL). PARTIAL RESULTS SHOWED A SCAB FORMATION AT DAY 14 IN ALL ANIMALS FROM GROUPS 1, 2, AND 3. NO SIGNS OF WOUND INFECTION WERE PRESENTED. ON DAY 14, ALL WOUNDS WERE RE-EPITHELIALIZED AND GRANULATION TISSUE WAS THICKER IN GROUP 2. IT COULD BE CONCLUDED THAT THE BILAYER SCAFFOLD IS THUS A PROMISING MATRIX TO BE USED AS A SKIN SUBSTITUTE. HOWEVER, IT WILL BE NECESSARY TO COMPLETE THE SAMPLE SIZE FOR EACH GROUP AND REALIZE HISTOLOGICAL AND IMMUNOENZYMATIC ASSAYS TO BETTER UNDERSTAND THE TISSUE REGENERATION PROCESS.

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Conference papers on the topic "3D co-cultures"

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Heaster, Tiffany M., Jiaquan Yu, Margaret Edman, David J. Beebe, and Melissa C. Skala. "Abstract 2785: Metabolic autofluorescence microscopy of 3D microscale macrophage-tumor co-cultures." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-2785.

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Heaster, Tiffany M., Jiaquan Yu, Margaret Edman, David J. Beebe, and Melissa C. Skala. "Abstract 2785: Metabolic autofluorescence microscopy of 3D microscale macrophage-tumor co-cultures." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-2785.

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Giangreco, Angeline Antonio, and Larisa Nonn. "Abstract 584: Vitamin D suppresses pro-inflammatory cytokines and microRNAs in 3D prostatic co-cultures." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-584.

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Thierauf, Julia C., Luca Engelmann, Natalia Koerich Laureano, Hans-Jürgen Stark, Elena-Sophie Prigge, Dominik Horn, Kolja Freier, et al. "Abstract 336: Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinoma." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-336.

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Daszkiewicz, Lidia, Gera Goverse, Nataliia Beztsinna, Marjan van de Merbel, Benjamin Visser, Tomas Veenendaal, Emma Spanjaard, Kuan Yan, and Leo Price. "Abstract P113: Image-based quantification of immunotherapeutic effect on the tumor-immune interactions in 3D co-cultures." In Abstracts: AACR-NCI-EORTC Virtual International Conference on Molecular Targets and Cancer Therapeutics; October 7-10, 2021. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1535-7163.targ-21-p113.

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Constantinou, Pamela E., Brian J. Engel, Lindsey K. Sablatura, Nathaniel J. Doty, Daniel D. Carson, Mary C. Farach-Carson, Daniel A. Harrington, and Thomas I. Zarembinski. "Abstract 604: A multi-layered, hydrogel system for automated 3D high throughput drug screening of cancer-stromal cell co-cultures." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-604.

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Kim, Minwook, Jason A. Burdick, and Robert L. Mauck. "Influence of Chondrocyte Zone on Co-Cultures With Mesenchymal Stem Cells in HA Hydrogels for Cartilage Tissue Engineering." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80859.

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Mesenchymal stem cells (MSCs) are an attractive cell type for cartilage tissue engineering in that they can undergo chondrogenesis in a variety of 3D contexts [1]. Focused efforts in MSC-based cartilage tissue engineering have recently culminated in the formation of biologic materials possessing biochemical and functional mechanical properties that match that of the native tissue [2]. These approaches generally involve the continuous or intermittent application of pro-chondrogenic growth factors during in vitro culture. For example, in one recent study, we showed robust construct maturation in MSC-seeded hyaluronic acid (HA) hydrogels transiently exposed to high levels of TGF-β3 [3]. Despite the promise of this approach, MSCs are a multipotent cell type and retain a predilection towards hypertrophic phenotypic conversion (i.e., bone formation) when removed from a pro-chondrogenic environment (e.g., in vivo implantation). Indeed, even in a chondrogenic environment, many MSC-based cultures express pre-hypertrophic markers, including type X collagen, MMP13, and alkaline phosphatase [4]. To address this issue, recent studies have investigated co-culture of human articular chondrocytes and MSCs in both pellet and hydrogel environments. Chondrocytes appear to enhance the initial efficiency of MSC chondrogenic conversion, as well as limit hypertrophic changes in some instances (potentially via secretion of PTHrP and/or other factors) [5–7]. While these findings are intriguing, articular cartilage has a unique depth-dependent morphology including zonal differences in chondrocyte identity. Ng et al. showed that zonal chondrocytes seeded in a bi-layered agarose hydrogel construct can recreate depth-dependent cellular and mechanical heterogeneity, suggesting that these identities are retained with transfer to 3D culture systems [8]. Further, Cheng et al. showed that differences in matrix accumulation and hypertrophy in zonal chondrocytes was controlled by bone morphogenic protein [9]. To determine whether differences in zonal chondrocyte identity influences MSC fate decisions, we evaluated functional properties and phenotypic stability in photocrosslinked hyaluronic acid (HA) hydrogels using distinct, zonal chondrocyte cell fractions co-cultured with bone marrow derived MSCs.

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Ji, Kyungmin, Zhiguo Zhao, Kamiar Moin, Yong Xu, and Bonnie F. Sloane. "Abstract B65: Live-cell imaging of 3D/4D parallel co-cultures of breast carcinoma cells and breast fibroblasts in tissue architecture and microenvironment engineering (TAME) chambers." In Abstracts: AACR Special Conference: Advances in Breast Cancer; October 17-20, 2015; Bellevue, WA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1557-3125.advbc15-b65.

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Papadopoulou, Nektaria, Pam Collier, Elisabeth Whelband, Anna M. Grabowska, and Susan A. Watson. "Abstract 4280: Differential influence of naïve & tumour-conditioned mesenchymal stem cells on the growth of human epithelial cells in in vitro monolayer co-cultures &3D hollow fibre systems." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-4280.

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Cantini, Marco, Gianfranco B. Fiore, Alberto Redaelli, and Monica Soncini. "CFD-Aided Design of a Dynamic Culture System for the Co-Culture of Adherent and Non-Adherent Cells." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206431.

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Haematopoietic stem cell (HSC) transplantation has been widely used to treat patients that have undergone high-dose chemotherapy or radiotherapy for haematological or non-haematological malignancies, and is currently investigated for the treatment of several other pathologic conditions. Nevertheless, present and expected clinical applications are hindered by the shortage of cells available for transplantation. Hence, many researchers have attempted to achieve an in vitro expansion of HSCs, using different experimental set-ups and approaches, which range from traditional static monolayer cultures to three-dimensional (3D) dynamic systems. Specifically, several bioreactor systems have been proposed, including perfusion chambers, stirred, rotating, hollow fiber, and packed bed reactors [1]. Taken together, literature studies suggest that a dynamic 3D culture system may provide superior expansion of HSCs.

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