Relevant bibliographies by topics / 3D cell

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic '3D cell'

Author: Grafiati
Published: 14 March 2023

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Journal articles on the topic "3D cell"

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Bikmulina, Polina, Nastasia Kosheleva, Yuri Efremov, Artem Antoshin, Zahra Heydari, Valentina Kapustina, Valery Royuk, et al. "3D or not 3D: a guide to assess cell viability in 3D cell systems." Soft Matter 18, no. 11 (2022): 2222–33. http://dx.doi.org/10.1039/d2sm00018k.

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The study aims at revealing the influence of particular 3D cell systems’ parameters such as the components’ concentration, gel thickness, cell density, on the cell viability and applicability of standard assays based on different cell properties.
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Ylostalo, Joni H. "3D Stem Cell Culture." Cells 9, no. 10 (September 27, 2020): 2178. http://dx.doi.org/10.3390/cells9102178.

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Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seeking optimal stem cell preparations for basic research and clinical applications, and these might be attainable through 3D culture of stem cells. The 3D cultures recapitulate the in vivo cell-to-cell and cell-to-matrix interactions more effectively, and the cells in 3D cultures exhibit many unique and desirable characteristics. The culture of stem cells in 3D may employ various matrices or scaffolds, in addition to the cells, to support the complex structures. The goal of this Special Issue is to bring together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cells and culture techniques, and also highlight stem cell preparations for possible novel therapeutic applications.
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Souza, Rhonda F., Robert E. Schwartz, and Hiroshi Mashimo. "Esophageal stem cells and 3D-cell culture models." Annals of the New York Academy of Sciences 1232, no. 1 (September 2011): 316–22. http://dx.doi.org/10.1111/j.1749-6632.2011.06070.x.

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Sapet, Cédric, Cécile Formosa, Flavie Sicard, Elodie Bertosio, Olivier Zelphati, and Nicolas Laurent. "3D-fection: cell transfection within 3D scaffolds and hydrogels." Therapeutic Delivery 4, no. 6 (June 2013): 673–85. http://dx.doi.org/10.4155/tde.13.36.

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Kiberstis, P. A. "Heart Cell Signaling in 3D." Science Signaling 3, no. 115 (March 30, 2010): ec93-ec93. http://dx.doi.org/10.1126/scisignal.3115ec93.

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Bouchet, Benjamin P., and Anna Akhmanova. "Microtubules in 3D cell motility." Journal of Cell Science 130, no. 1 (January 1, 2017): 39–50. http://dx.doi.org/10.1242/jcs.189431.

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Harunaga, Jill S., and Kenneth M. Yamada. "Cell-matrix adhesions in 3D." Matrix Biology 30, no. 7-8 (September 2011): 363–68. http://dx.doi.org/10.1016/j.matbio.2011.06.001.

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Glaser, Vicki. "Novel 3D Cell Culture Systems." Genetic Engineering & Biotechnology News 33, no. 16 (September 15, 2013): 1, 22, 24–25. http://dx.doi.org/10.1089/gen.33.16.09.

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Even-Ram, Sharona, and Kenneth M. Yamada. "Cell migration in 3D matrix." Current Opinion in Cell Biology 17, no. 5 (October 2005): 524–32. http://dx.doi.org/10.1016/j.ceb.2005.08.015.

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Rangarajan, Rajagopal, and Muhammad H. Zaman. "Modeling cell migration in 3D." Cell Adhesion & Migration 2, no. 2 (April 2008): 106–9. http://dx.doi.org/10.4161/cam.2.2.6211.

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Dissertations / Theses on the topic "3D cell"

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Timp, Winston (Winston G. ). "Study of cell-cell communication using 3D living cell microarrays." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/42059.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007.
This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Includes bibliographical references (p. 135-152).
Cellular behavior is not dictated solely from within; it is also guided by a myriad of external cues. If cells are removed from their natural environment, apart from the microenvironment and social context they are accustomed to, it is difficult to study their behavior in any meaningful way. To that end, I describe a method for using optical trapping for positioning cells with submicron accuracy in three dimensions, then encapsulating them in hydrogel, in order to mimic the in vivo microenvironment. This process has been carefully optimized for cell viability, checking both prokaryotic and eukaryotic cells for membrane integrity and metabolic activity. To demonstrate the utility of this system, I have looked at a model "quorum sensing" system in Vibrio Fischeri, which operates by the emission and detection of a small chemical signal, an acyl-homoserine lactone. Through synthetic biology, I have engineered plasmids which express "sending" and "receiving" genes. Bacteria containing these plasmids were formed into complex 3D patterns, designed to assay signaling response. The gene expression of the bacteria was tracked over time using fluorescent proteins as reporters. A model for this system was composed using a finite element method to simulate signal transport through the hydrogel, and simple mass-action kinetic equations to simulate the resulting protein expression over time.
by Winston Timp.
Ph.D.
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Valldeperas, Roger. "Production Cell Simulation Visualization in 3D." Thesis, Linnéuniversitetet, Institutionen för datavetenskap (DV), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-27964.

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The thesis explains the development process of a production cell simulation in 3D implemented using Unity3D. The developed simulation communicates with existing control software and aims to test this control software in a 3D environment with physics simulation. The final result includes 3D models and also works as a visualization since it allows us to present the control system, and this visualization can be viewed using most web browsers. The thesis also includes a brief study and comparison between currently popular game engines to choose an appropriate option for this project.This is a project in collaboration with a local company (ARiSA) and has a high practical relevance.
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Capra, J. (Janne). "Differentiation and malignant transformation of epithelial cells:3D cell culture models." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526218236.

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Abstract The epithelial cells form barriers that compartmentalize the organs. Carcinomas are cancers stemming from epithelial cells and are the most common cancer type. The aim of this study was to understand the differentiation and malignant transformation of epithelial Madin-Darby canine kidney (MDCK) cells and to analyse the electrophysiological parameters which regulate their transport capacity. Emphasis was placed on comparing different culture environments, both in 2D and 3D. First, the effects of drugs or basal extracellular fluid composition on MDCK cell, cyst and lumen volumes were analysed using time-lapse microscopy. The results showed that MDCK cells were capable of both water secretion and reabsorption. The cells were able to perform these functions in a hyperpolarizing or depolarizing environment; change in osmolality of basal fluid was not required. Taken together, these results validate MDCK cells as a good basic model for studying kidney function. Next, the aim was to analyse the effect of 2D and 3D culture environments on the gene expression of untransformed MDCK and temperature sensitive ts-Src -transformed MDCK cells and the changes a single oncogene can induce. Microarray analysis revealed a decrease in the expression of survivin, an inhibitor of apoptosis protein, when switching the untransformed cells from 2D environment to 3D. This downregulation of survivin occurs in adult tissues as well, indicating that the cells grown in 3D are closer to the in vivo state than 2D cells. Src oncogene induced disintegration of cell junctions, but did not downregulate E-cadherin expression. The last part was to study further the factors controlling survivin expression and its significance to cell survival. MDCK cells grown in 3D did not suffer apoptosis if the cells remained in contact with the extracellular matrix. If MDCK cells were denied of ECM contacts they were more susceptible to apoptosis than survivin-expressing ts-Src MDCK cells. Finally, if cells were denied of cell-cell junctions, cells lacking survivin suffered apoptosis even though they had proper cell-matrix contacts. Taken together, these results highlighted the importance of cellular contacts to the cells: MDCK cells needed ECM contacts to differentiate and cell-cell contacts to avoid apoptosis
Tiivistelmä Epiteelisolut ovat erikoistuneet toimimaan rajapintana elimen ja ympäristön välillä. Ihmisten yleisin syöpä on epiteelisoluista alkunsa saanut karsinooma. Tämän tutkimuksen tarkoituksena oli ymmärtää Madin-Darby-koiran munuaisen solujen (MDCK) erilaistumista ja pahanlaatuistumista sekä analysoida sähköfysiologisia tekijöitä, jotka säätelevät näiden solujen kuljetustoimintaa. Erityisenä kiinnostuksen kohteena oli erilaisten kasvuympäristöjen vertailu. Farmakologisten aineiden tai basaalisen, solunulkopuolisen nesteen koostumuksen vaikutusta MDCK-solujen, -kystan sekä luumenin kokoon tutkittiin valomikroskooppisten aikasarjojen avulla. Tulokset osoittivat MDCK-solujen olevan kykeneviä sekä veden eritykseen että absorptioon, niin hyperpolarisoivassa kuin depolarisoivassakin ympäristössä. Basaalisen nesteen osmolaliteetin muutosta ei tarvittu. Nämä tulokset osoittavat MDCK-solujen olevan hyvä munuaisen tutkimuksen perusmalli. Seuraavaksi analysoitiin kaksi- ja kolmiulotteisten (2D ja 3D) viljely-ympäristöjen vaikutusta ei-transformoitujen MDCK-solujen ja lämpötilaherkkien ts-Src-transformoitujen MDCK-solujen geenien ilmentymiseen sekä yhden onkogeenin aktivoimisen aikaansaamia muutoksia. Microarray-analyysi osoitti apoptoosin estäjän, surviviinin, ilmentymisen vähenemisen, kun kasvuympäristö vaihdettiin 2D-ympäristöstä 3D-ympäristöön. Koska surviviinin väheneminen on normaali tapahtuma aikuisissa kudoksissa, voitiin todeta, että 3D-ympäristössä kasvatetut solut ovat lähempänä luonnonmukaista olotilaa kuin 2D-ympäristössä kasvaneet. Src-onkogeeni sai aikaan soluliitosten hajoamisen, mutta ei vähentänyt E-kadheriinin ilmentymistä. Tutkimuksen viimeinen osa keskittyi surviviinin ilmentymistä säätelevien tekijöiden analysoimiseen ja surviviinin merkitykseen solujen eloonjäämiselle. 3D-ympäristössä kasvaneet MDCK-solut eivät kärsineet apoptoosista edellyttäen, että solut pysyivät kosketuksissa soluväliaineeseen. Jos solut irtautuivat soluväliaineesta, ne päätyivät herkemmin apoptoosiin kuin surviviinia ilmentävät ts-Src MDCK-solut. Mikäli solujen väliset liitokset pakotettiin avautumaan, solut joutuivat apoptoosiin, vaikka ne olivat kosketuksissa soluväliaineeseen. Yhteenvetona nämä tulokset korostavat solujen kontaktien merkitystä: MDCK-solut tarvitsevat soluväliainekontakteja erilaistumiseen ja solujen välisiä kontakteja välttyäkseen apoptoosilta
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Godeau, Amélie. "Cyclic contractions contribute to 3D cell motility." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAF038/document.

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La motilité des cellules est un phénomène fondamental en biologie souvent étudié sur des surfaces planes, conditions peu physiologiques. Nous avons analysé la migration cellulaire dans une matrice cellulaire 3D contenant de la fibronectine fluorescente. Nous démontrons que les cellules y sont confinées, et déforment leur environnement de manière cyclique avec une période de ~14 min avec deux centres de contractions à l’avant et à l’arrière de la cellule qui contractent avec un déphasage de ~3.5 min. Une perturbation de ces cycles entraîne une réduction de la motilité. Par l’utilisation d’inhibiteurs spécifiques, nous avons identifié l’acto-myosine comme étant l’acteur principal de ce phénomène. En imposant des contractions-relaxations locales par ablations laser, nous avons déclenché la motilité cellulaire ce qui confirme notre hypothèse. L’ensemble de cette étude met en évidence un nouveau mécanisme fondamental de dynamique cellulaire impliqué dans le mouvement des cellules
Cell motility is an important process in Biology. It is mainly studied on 2D planar surfaces, whereas cells experience a confining 3D environment in vivo. We prepared a 3D Cell Derived Matrix (CDM) labeled with fluorescently labeled fibronectin, and strikingly cells managed to deform the matrix with specific patterns : contractions occur cyclically with two contraction centers at the front and at the back of the cell, with a period of ~14 min and a phase shift of ~3.5 min. These cycles enable cells to optimally migrate through the CDM, as perturbation of cycles led to reduced motility. Acto-myosin was established to be the driving actor of these cycles, by using specific inhibitors. We were able to trigger cell motility externally with local laser ablations, which supports this framework of two alternating contractions involved in motion. Altogether, this study reveals a new mechanism of dynamic cellular behaviour linked to cell motility
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Atefi, Ehsan. "Aqueous Biphasic 3D Cell Culture Micro-Technology." University of Akron / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1443112692.

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Rajendran, Balakumar. "3D Agent Based Model of Cell Growth." Cincinnati, Ohio : University of Cincinnati, 2009. http://www.ohiolink.edu/etd/view.cgi?acc_num=ucin1231358178.

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Thesis (M.S.)--University of Cincinnati, 2009.
Advisors: Carla Purdy PhD (Committee Chair), Daria Narmoneva PhD (Committee Member), Ali Minai PhD (Committee Member). Title from electronic thesis title page (viewed April 30, 2009). Includes abstract. Keywords: Agent based modeling; cell growth; three dimensional. Includes bibliographical references.
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CAPRETTINI, VALERIA. "Cell membrane interactions with 3D multifunctional nanostructures." Doctoral thesis, Università degli studi di Genova, 2018. http://hdl.handle.net/11567/930970.

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In recent years, cells studies are often carried out with the help of three-dimensional (3D) nanostructured substrates. These kind of substrates can be useful in assisting the access in to the intracellular compartment and the delivery of molecules through holes or nanochannels, as well as in increasing the contact area of a cell with an electrode during electrical recordings.
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Tabriz, Atabak Ghanizadeh. "3D biofabrication of cell-laden alginate hydrogel structures." Thesis, Heriot-Watt University, 2017. http://hdl.handle.net/10399/3370.

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Biofabrication has been receiving a great deal of attention in tissue engineering and regenerative medicine either by manual or automated processes. Different automated biofabrication techniques have been used to produce cell-laden alginate hydrogel structures, especially bioprinting approaches. , These approaches have been limited to 2D or simple 3D structures, however. In this thesis, a new extrusion-based bioprinting technique and a new simple, manual 3D biofabrication method are presented to culture cells in 3D. These methods do not rely on any complex fabrication methods. The bioprinting technique was developed to produce more complex alginate hydrogel structures. This was achieved by dividing the alginate hydrogel cross-linking process into 3 stages: primary calcium ion cross-linking for printability of the gel, secondary calcium cross-linking for rigidity of the alginate hydrogel immediately after printing and tertiary barium ion cross-linking for the long-term stability of the alginate hydrogel in the culture medium. Simple 3D structures including tubes were first printed to ensure the feasibility of the bioprinting technique. Complex 3D structures, such as branched vascular structures, were subsequently printed successfully. The static stiffness of the alginate hydrogel after printing was 20.18 ± 1.62 kPa which was rigid enough to sustain the integrity of the complex 3D alginate hydrogel structure during the printing. The addition of 60 mM barium chloride was found to significantly extend the stability of the cross-linked alginate hydrogel from 3 days to beyond 11 days without compromising the cellular viability. The results based on cell bioprinting suggested that the viability of U87-MG cells was 92.94 ± 0.91 % immediately after bioprinting. Cell viability was maintained above 88 ± 4.3 % in the alginate hydrogel over a period of 11 days. On the other hand, the manual biofabrication approach developed in this thesis enabled the fabrication of scalable 3D cell-laden hydrogel structures easily, without complex machinery. The technique could be carried out using only apparatus available in a typical cell biology laboratory. The fabrication method would involve micro coating cell-laden hydrogels covering the surface of a metal bar by dipping into cross-linking reagent CaCl2 or BaCl2, to form hollow tubular structures. This method could be used to form single- or multi-layered tubular structures. This fabrication method has incorporated the use alginate hydrogel as the primary biomaterial and secondary biomaterial could be added depending on the desired application. The feasibility of this method has been demonstrated by showing the cell survival rate and normal responsiveness of cells within these tubular structures using mouse dermal embryonic fibroblast cells and human embryonic kidney 293 cells containing a tetracycline responsive red fluorescence protein (tHEK cells). By adjusting the fabrication protocol, complex hollow alginate hydrogel structures could be generated.
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Pasturel, Aurélien. "Tailoring common hydrogels into 3D cell culture templates." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0302.

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L’ingénierie d’hydrogels ; leur structuration et fonctionnalisation à l’échelle cellulaire, est une étape clé pour aboutir à de modèles in-vitro plus physiologiques. À ce jour, elle reste difficile car ces matériaux polymères, mous et riches en eau, sont souvent trop fragiles pour la micro-fabrication traditionnelle. Pour pallier à ce fait, nous avons combinée illumination ultraviolette structurée et chambres de réaction perméables au gaz nous offrant la maitrise sur la distribution de photons, les réactifs et les gaz présents à chaque instant et en tout point d’un champ d’illumination. Nous pouvons ainsi contrôler une photochimie adaptée aux hydrogels les plus répandus et structurer, décorer ou liquéfier ces matériaux. Ensemble ces trois opérations forment une boite à outil complète adaptée aux substrats les plus communs que sont Matrigel, Agar, Poly(acrylamide) et Poly(éthylène-glycol). Nous avons par la suite fabriqué des micro-niches en hydrogel permettant la culture standardisée de lignées cellulaire et de neurones primaires soit par adhésion sur des topographies ou par auto-organisation en sphéroïdes. Ceci démontre que la plateforme est à même de répondre à des enjeux importants de culture cellulaire tridimensionnelle
Tailoring hydrogels into biomimetic templates represents a crucial step to build better in-vitro models but it is to date still challenging. Indeed, these synthetic or natural polymeric networks are often so frail they can’t be processed through standard micro-fabrication. Here, we combine a ultra-violet pattern projector with gas permeable microreactors to control gas, reagents and photon distribution and in fine, the reaction kinetics in space and time. Doing so, enabled a generic chemistry that can structure, liquefy or decorate (locally functionalize) common hydrogels. Altogether these three hydrogel engineering operations form a flexible toolbox that supports the most commonly used hydrogels: i.e. Matrigel, Agar-agar, poly(ethylene-glycol) and poly(acryl-amide). We successfully applied this solution to grow cells into standardized micro-niches demonstrating that it can readily address cell culture challenges such has controlled adhesion on topographical structures, standardization of spheroids or culture on shaped Matrigel
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Chetty, Avashnee Shamparkesh. "Thermoresponsive 3D scaffolds for non-invasive cell culture." Thesis, University of Pretoria, 2012. http://hdl.handle.net/2263/25463.

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Conventionally, adherent cells are cultured in vitro using flat 2D cell culture trays. However the 2D cell culture method is tedious, unreliable and does not replicate the complexity of the 3D dynamic environment of native tissue. Nowadays 3D scaffolds can be used to culture cells. However a number of challenges still exist, including the need for destructive enzymes to release confluent cells. Poly(Nisopropylacrylamide) (PNIPAAm), a temperature responsive polymer, has revolutionised the cell culture fraternity by providing a non-invasive means of harvesting adherent cells, whereby confluent cells can be spontaneously released by simply cooling the cell culture medium and without requiring enzymes. While PNIPAAm monolayer cell culturing is a promising tool for engineering cell sheets, the current technology is largely limited to the use of flat 2D substrates, which lacks structural and organisational cues for cells. The aim of this project was to develop a 3D PNIPAAm scaffold which could be used efficiently for non-invasive 3D culture of adherent cells. This project was divided into three phases: Phase 1 (preliminary phase) involved development and characterisation of cross-linked PNIPAAm hydrogels; Phase 2 involved development and characterisation of PNIPAAm grafted 3D non-woven scaffolds, while Phase 3 focused on showing proof of concept for non-invasive temperature-induced cell culture from the 3D PNIPAAm grafted scaffolds. In Phase 1, PNIPAAm was cross-linked with N,N’-methylene-bis-acrylamide (MBA) using solution free-radical polymerisation to form P(PNIPAAm-co-MBA) hydrogels. A broad cross-link density (i.e. 1.1 - 9.1 Mol% MBA) was investigated, and the effect of using mixed solvents as the co-polymerisation medium. The P(PNIPAAm-co-MBA) gels proved unsuitable as a robust cell culture matrix, due to poor porosity, slow swelling/deswelling and poor mechanical properties. Subsequently, in Phase 2, polypropylene (PP), polyethylene terephthalate (PET), and nylon fibers were processed into highly porous non-woven fabric (NWF) scaffolds using a needle-punching technology. The NWF scaffolds were grafted with PNIPAAm using oxyfluorination-assisted graft polymerisation (OAGP). The OAGP method involved a 2 step process whereby the NWF was first fluorinated (direct fluorination or oxyfluorination) to introduce new functional groups on the fibre surface. The functionalised NWF scaffolds were then graft-polymerised with NIPAAm in an aqueous medium using ammonium persulphate as the initiator. Following oxyfluorination, new functional groups were detected on the surface of the NWF scaffolds, which included C-OH; C=O; CH2-CHF, and CHF-CHF. PP and nylon were both easily modified by oxyfluorination, while PET displayed very little changes to its surface groups. Improved wetting and swelling in water was observed for the oxyfluorinated polymers compared to pure NWF scaffolds. PP NWF showed the highest graft yield followed by nylon and then PET. PNIPAAm graft yield on the PP NWF was ~24 ±6 μg/cm2 on grafted pre-oxyfluorinated NWF when APS was used; which was found to be significantly higher compared to when pre-oxyfluorinated NWF was used without initiator (9 ±6 μg/cm2, p= 1.7x10-7); or when grafting was on pure PP with APS (2 ±0.3 μg/cm2, p = 8.4x10-12). This corresponded to an average PNIPAAm layer thickness of ~220 ±54 nm; 92 ± 60 nm; and 19 ± 3 nm respectively. Scanning electron microscopy (SEM) revealed a rough surface morphology and confinement of the PNIPAAm graft layer to the surface of the fibers when oxyfluorinated NWF scaffolds were used, however when pure NWF scaffolds were used during grafting, homopolymerisation was observed as a loosely bound layer on the NWF surface. The OAGP method did not affect the crystalline phase of bulk PP as was determined by X-ray diffraction (XRD), however, twin-melting thermal peaks were detected from DSC for the oxyfluorinated PP and PP-g-PNIPAAm NWF which possibly indicated crystal defects. Contact angle studies and microcalorimetric DSC showed that the PP-g-PNIPAAm NWF scaffolds exhibited thermoresponsive behaviour. Using the 2,2-Diphenyl-1-1-picrylhydrazyl (DPPH) radical method and electron-spin resonance (ESR), peroxides, as well as trapped long-lived peroxy radicals were identified on the surface of the oxyfluorinated PP NWF, which are believed to be instrumental in initiating graft polymerisation from the NWF. A free radical mechanism which is diffusion controlled was proposed for the OAGP method with initiation via peroxy radicals (RO•), as well as SO4•- and OH• radicals, whereby the latter result from decomposition of APS. In Phase 3 of this study, proof-of-concept is demonstrated for use of the PNIPAAm grafted NWF scaffolds in non-invasive culture of hepatocytes. Studies demonstrated that hepatocyte cells attached onto the 3D PNIPAAm scaffolds and remained viable in culture over long periods. The cells were released spontaneously and non-destructively as 3D multi-cellular constructs by simply cooling the cell culture medium from 37°C to 20°C, without requiring destructive enzymes. The PP-g- PNIPAAm NWF scaffolds performed the best in 3D cell culture. Additionally the CSIR is developing a thermo responsive 3D (T3D) cell culturing device, whereby the 3D thermo responsive NWF scaffolds are used in the bioreactor for cell culture. Temperature-induced cell release was also verified from the 3D Thermo responsive scaffolds in the bioreactor. This technology could lead to significant advances in improving the reliability of the in vitro cell culture model. Please cite as follows: Chetty, AS 2012, Thermoresponsive 3D scaffolds for non-invasive cell culture, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-06112013-151344/ > D13/4/713/ag
Thesis (PhD)--University of Pretoria, 2012.
Chemical Engineering
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Books on the topic "3D cell"

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Koledova, Zuzana, ed. 3D Cell Culture. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7021-6.

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Haycock, John W., ed. 3D Cell Culture. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-60761-984-0.

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Kasper, Cornelia, Dominik Egger, and Antonina Lavrentieva, eds. Basic Concepts on 3D Cell Culture. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-66749-8.

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Przyborski, Stefan, ed. Technology Platforms for 3D Cell Culture. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781118851647.

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3D cell culture: Methods and protocols. New York: Humana Press/Springer, 2011.

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Haycock, John W. 3D cell culture: Methods and protocols. New York: Humana Press/Springer, 2011.

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Dmitriev, Ruslan I., ed. Multi-Parametric Live Cell Microscopy of 3D Tissue Models. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-67358-5.

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Huang, Liang, and Wenhui Wang. 3D Electro-Rotation of Single Cells. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-031-01666-0.

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Dutta, Ranjna C., and Aroop K. Dutta. 3D Cell Culture. Jenny Stanford Publishing, 2018. http://dx.doi.org/10.1201/b22417.

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3D Stem Cell Culture. MDPI, 2021. http://dx.doi.org/10.3390/books978-3-03943-804-4.

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Book chapters on the topic "3D cell"

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Moissoglu, Konstadinos, Stephen J. Lockett, and Stavroula Mili. "Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids." In Cell Migration in Three Dimensions, 263–80. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_16.

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AbstractLocalization of mRNAs at the front of migrating cells is a widely used mechanism that functionally supports efficient cell movement. It is observed in single cells on two-dimensional surfaces, as well as in multicellular three-dimensional (3D) structures and in tissue in vivo. 3D multicellular cultures can reveal how the topology of the extracellular matrix and cell-cell contacts influence subcellular mRNA distributions. Here we describe a method for mRNA imaging in an inducible system of collective cancer cell invasion. MDA-MB-231 cancer cell spheroids are embedded in Matrigel, induced to invade, and processed to image mRNAs with single-molecule sensitivity. An analysis algorithm is used to quantify and compare mRNA distributions at the front of invasive leader cells. The approach can be easily adapted and applied to analyze RNA distributions in additional settings where cells polarize along a linear axis.
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Duarte Campos, Daniela F., and Andreas Blaeser. "3D-Bioprinting." In Basic Concepts on 3D Cell Culture, 201–32. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-66749-8_9.

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Koch, Lothar, Andrea Deiwick, and Boris Chichkov. "Laser-Based Cell Printing." In 3D Printing and Biofabrication, 1–27. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-40498-1_11-1.

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Koch, Lothar, Andrea Deiwick, and Boris Chichkov. "Laser-Based Cell Printing." In 3D Printing and Biofabrication, 303–29. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-45444-3_11.

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Leberfinger, Ashley N., Kazim Kerim Moncal, Dino J. Ravnic, and Ibrahim T. Ozbolat. "3D Printing for Cell Therapy Applications." In Cell Therapy, 227–48. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-57153-9_11.

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Jacobs, Kathryn A., and Julie Gavard. "3D Endothelial Cell Migration." In Methods in Molecular Biology, 51–58. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7701-7_6.

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Evans, David M., and Beverly A. Teicher. "3D Cell Culture Models." In Molecular and Translational Medicine, 251–75. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-57424-0_19.

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Rani, Madhu, Annu Devi, Shashi Prakash Singh, Rashmi Kumari, and Anil Kumar. "3D Cell Culture Techniques." In Techniques in Life Science and Biomedicine for the Non-Expert, 197–212. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-19485-6_14.

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Tytgat, L., S. Baudis, H. Ottevaere, R. Liska, H. Thienpont, P. Dubruel, and S. Van Vlierberghe. "Photopolymerizable Materials for Cell Encapsulation." In 3D Printing and Biofabrication, 1–43. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-40498-1_15-1.

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Tytgat, L., Stefan Baudis, H. Ottevaere, R. Liska, H. Thienpont, P. Dubruel, and S. Van Vlierberghe. "Photopolymerizable Materials for Cell Encapsulation." In 3D Printing and Biofabrication, 353–96. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-45444-3_15.

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Conference papers on the topic "3D cell"

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Matsusaki, Michiya, and Mitsuru Akashi. "3D-cell assembly by control of cell surfaces." In 2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2015. http://dx.doi.org/10.1109/mhs.2015.7438309.

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Yan, Karen Chang, Kamila Paluch, Kalyani Nair, and Wei Sun. "Effects of Process Parameters on Cell Damage in a 3D Cell Printing Process." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-11528.

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Various types of bio-fabrication methods have been developed to manufacture products with living cells incorporated via mechanical means. One of fundamental questions that need to be answered is whether cells remain viable and/or functional when subjected to these mechanical disturbances. In this paper, we focus on a 3D cell-printing process via pressure induced deposition. Our experimental studies show that process parameters such as pressure applied and nozzle size affect the cell viability. Given that the cells are suspended in the alginate solution during the printing process, Computational Fluid Dynamic (CFD) analysis is employed to model the pressure-driven flow system and determine the local environment that the living cells are in under varying process parameters. Effects of obtained wall shear stress and exposure time are examined in terms of cell damage based on the corresponding experimental data.
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Farsari, Maria, George Flamourakis, Ioannis Spanos, Vasileia Melissinaki, Zacharias Vangelatos, Costas P. Grigoropoulos, and Anthi Ranella. "3D auxetic metamaterials as scaffolds for cell growth (Conference Presentation)." In Laser 3D Manufacturing VII, edited by Henry Helvajian, Bo Gu, and Hongqiang Chen. SPIE, 2020. http://dx.doi.org/10.1117/12.2543752.

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Kryou, Christina, Panos Karakaidos, Symeon Papazoglou, Apostolos Klinakis, and Ioanna Zergioti. "Laser bioprinting and laser photo-crosslinking of cell-laden bioinks." In Laser 3D Manufacturing IX, edited by Henry Helvajian, Bo Gu, and Hongqiang Chen. SPIE, 2022. http://dx.doi.org/10.1117/12.2607113.

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Lai, Yi-Han, and Shih-Kang Fan. "Electromolding for 3D cell culture." In 2015 IEEE 10th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2015. http://dx.doi.org/10.1109/nems.2015.7147424.

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Campana, Kimberly A., Eric Y. Shin, Beverly Z. Waisner, and Sherry L. Voytik-Harbin. "3D Cell Shape and Cell Fate are Regulated by the Dynamic Micro-Mechanical Properties of the Cell-ECM Interface." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176626.

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Mechanobiology is an interdisciplinary field that focuses on predicting and understanding cellular responses to mechanical loads. The extracellular matrix (ECM) represents a macromolecular framework that naturally imparts structural support and spatial organization for resident cells. The ECM also participates in the communication and transfer of mechanical loads to cells, in part, via integrin attachment to the cytoskeleton (CSK). Recently, using a tissue model in which cells are embedded in a 3D collagen ECM, we have shown that fundamental cell behaviors, including morphology, proliferation, contractility, and ECM remodeling properties, can be modulated by varying 3D microstructural organization and mechanical properties of the surrounding collagen fibrils[1]. While these and other results demonstrate the critical role played by the ECM in regulating cell behavior, the mechanical-based mechanisms underlying these critical cell-ECM interactions have yet to be fully elucidated [2].
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Isu, Giuseppe, Diana Massai, Giulia Cerino, Diego Gallo, Cristina Bignardi, Alberto Audenino, and Umberto Morbiducci. "A Novel Perfusion Bioreactor for 3D Cell Culture in Microgravity Conditions." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14502.

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Cell suspension culture methods based on the generation of microgravity environment are widely used in regenerative medicine for (1) the production of native-like three-dimensional (3D) cell aggregates and engineered tissues [1,2,3], for (2) low cost scalable cell expansion and long-term cell viability maintenance [4,5], and for (3) guiding differentiation of stem cells (SCs) [6]. The generation of a microgravity environment for 3D cell cultures, mimicking the native environment, promotes spatial freedom, cell growth, cell-cell interaction and improves mass transfer and cell exposure to nutrients. Nowadays, microgravity cell cultures are obtained by using stirred or rotating bioreactors, but both devices suffer from limitations: stirring bioreactors generate non-physiological shear stresses, which could damage cultured cells, interfere with SC pluripotency, and limit reproducibility of the culture process; rotating bioreactors are expensive devices due to the complex technological solutions adopted for obtaining rotation [5].
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Hippler, Marc, Kai Weißenbruch, Enrico Lemma, Eva Blasco, Motomu Tanaka, Christopher Barner-Kowollik, Martin Bastmeyer, and Martin Wegener. "Stimuli-responsive 3D micro-scaffolds for single cell actuation (Conference Presentation)." In Laser 3D Manufacturing VII, edited by Henry Helvajian, Bo Gu, and Hongqiang Chen. SPIE, 2020. http://dx.doi.org/10.1117/12.2543477.

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Takeuchi, Masaru, Masahiro Nakajima, and Toshio Fukuda. "Cell culture inside thermoresponsive gels towards 3D cell structures." In 2013 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2013. http://dx.doi.org/10.1109/mhs.2013.6710478.

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Hoshino, Kenji, Sho Nabatame, Atsushi Nagate, and Teruya Fujii. "Inter-cell interference coordination by horizontal beamforming for small cells in 3D cell structure." In 2015 IEEE Wireless Communications and Networking Conference Workshops (WCNCW). IEEE, 2015. http://dx.doi.org/10.1109/wcncw.2015.7122582.

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Reports on the topic "3D cell"

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Malik, Abir, D. Lam, H. A. Enright, S. K. G. Peters, B. Petkus, and N. O. Fischer. Characterizing the Phenotypes of Brain Cells in a 3D Hydrogel Cell Culture Model. Office of Scientific and Technical Information (OSTI), August 2018. http://dx.doi.org/10.2172/1466140.

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Grebennikov, A. N., A. K. Zhitnik, and O. A. Zvenigorodskaya. Results of comparative RBMK neutron computation using VNIIEF codes (cell computation, 3D statics, 3D kinetics). Final report. Office of Scientific and Technical Information (OSTI), December 1995. http://dx.doi.org/10.2172/219464.

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Swanekamp, S. B., A. S. Richardson, I. Ritterdorf, J. W. Schumer, and B. V. Weber. Particle-in-Cell Simulations of Electromagnetic Power-Flow in a Complex 3D Geometry. Office of Scientific and Technical Information (OSTI), February 2018. http://dx.doi.org/10.2172/1422357.

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Kiefer, M. L., D. B. Seidel, R. S. Coats, J. P. Quintenz, T. D. Pointon, and W. A. Johnson. Architecture and computing philosophy of the QUICKSILVER, 3D, electromagnetic, particle-in-cell code. Office of Scientific and Technical Information (OSTI), January 1990. http://dx.doi.org/10.2172/7271685.

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Seidel, D. B., M. F. Pasik, M. L. Kiefer, D. J. Riley, and C. D. Turner. Advanced 3D electromagnetic and particle-in-cell modeling on structured/unstructured hybrid grids. Office of Scientific and Technical Information (OSTI), January 1998. http://dx.doi.org/10.2172/567511.

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Burris-Mog, Trevor John. 3D Particle-In-Cell Model of Axis-I: Cathode to Target Single-Code Development for Scorpius. Office of Scientific and Technical Information (OSTI), January 2019. http://dx.doi.org/10.2172/1492556.

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Dolgashev, Valery A. Simulations of Currents in X-Band Accelerator Structures Using 2D and 3D Particle-in-Cell Code. Office of Scientific and Technical Information (OSTI), August 2002. http://dx.doi.org/10.2172/799912.

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Mastro, Andrea M. Altering the Microenvironment to Promote Dormancy of Metastatic Breast Cancer Cell in a 3D Bone Culture System. Fort Belvoir, VA: Defense Technical Information Center, April 2014. http://dx.doi.org/10.21236/ada604844.

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Mastro, Andrea M., and Erwin Vogler. Altering the Microenvironment to Promote Dormancy of Metastatic Breast Cancer Cell in a 3D Bone Culture System. Fort Belvoir, VA: Defense Technical Information Center, April 2015. http://dx.doi.org/10.21236/ada621382.

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S. Ethier and Z. Lin. Porting the 3D Gyrokinetic Particle-in-cell Code GTC to the CRAY/NEC SX-6 Vector Architecture: Perspectives and Challenges. Office of Scientific and Technical Information (OSTI), September 2003. http://dx.doi.org/10.2172/815094.

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