Relevant bibliographies by topics / 2D-PAGE

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  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
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Academic literature on the topic '2D-PAGE'

Author: Grafiati
Published: 4 June 2021
Last updated: 11 March 2023

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Journal articles on the topic "2D-PAGE"

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Matera, Robert, Katalin V. Horvath, Hari Nair, Ernst J. Schaefer, and Bela F. Asztalos. "HDL Particle Measurement: Comparison of 5 Methods." Clinical Chemistry 64, no. 3 (March 1, 2018): 492–500. http://dx.doi.org/10.1373/clinchem.2017.277632.

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Abstract BACKGROUND HDL cell cholesterol efflux capacity has been documented as superior to HDL cholesterol (HDL-C) in predicting cardiovascular disease risk. HDL functions relate to its composition. Compositional assays are easier to perform and standardize than functional tests and are more practical for routine testing. Our goal was to compare measurements of HDL particles by 5 different separation methods. METHODS HDL subfractions were measured in 98 samples using vertical auto profiling (VAP), ion mobility (IM), nuclear magnetic resonance (NMR), native 2-dimensional gel electrophoresis (2D-PAGE), and pre-β1-ELISA. VAP measured cholesterol in large HDL2 and small HDL3; IM measured particle number directly in large, intermediate, and small HDL particles; NMR measured lipid signals in large, medium, and small HDL; 2D-PAGE measured apolipoprotein (apo) A-I in large (α1), medium (α2), small (α3–4), and pre-β1 HDL particles; and ELISA measured apoA-I in pre-β1-HDL. The data were normalized and compared using Passing–Bablok, Lin concordance, and Bland–Altman plot analyses. RESULTS With decreasing HDL-C concentration, NMR measured a gradually lower percentage of large HDL, compared with IM, VAP, and 2D-PAGE. In the lowest HDL-C tertile, NMR measured 8% of large HDL, compared with IM, 22%; VAP, 20%; and 2D-PAGE, 18%. There was strong discordance between 2D-PAGE and NMR in measuring medium HDL (R2 = 0.356; rc = 0.042) and small HDL (R2 = 0.376; rc = 0.040). The 2D-PAGE assay measured a significantly higher apoA-I concentration in pre-β1-HDL than the pre-β1-ELISA (9.8 vs 1.6 mg/dL; R2 = 0.246; rc = 0.130). CONCLUSIONS NMR agreed poorly with the other methods in measuring large HDL, particularly in low HDL-C individuals. Similarly, there was strong discordance in pre-β1-HDL measurements between the ELISA and 2D-PAGE assays.
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Vijayendran, Chandran, Sebastian Burgemeister, Karl Friehs, Karsten Niehaus, and Erwin Flaschel. "2DBase: 2D-PAGE database of Escherichia coli." Biochemical and Biophysical Research Communications 363, no. 3 (November 2007): 822–27. http://dx.doi.org/10.1016/j.bbrc.2007.09.050.

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Hanneken, Marina, Doreen Ackermann, Simone König, and Günter Thesseling. "Koordinatenbestimmung von Proteinpunkten in 2D-PAGE-Gelen." BIOspektrum 20, no. 6 (September 30, 2014): 655–57. http://dx.doi.org/10.1007/s12268-014-0503-5.

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Chorney, M. J., J. S. Tung, Y. Bushkin, and F. W. Shen. "Structural characteristics of Tla products." Journal of Experimental Medicine 162, no. 3 (September 1, 1985): 781–89. http://dx.doi.org/10.1084/jem.162.3.781.

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Biochemical study of thymus leukemia antigen (TL) from thymocytes of various Tla genotypes and from leukemia cells revealed features that, given present evidence, are peculiar to TL among class I products of the H-2:Qa:Tla region of chromosome 17. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of TL from thymocytes of all TL+ mouse strains, precipitated by anti-TL antiserum or monoclonal antibodies, showed two closely migrating bands of equal intensity in the heavy (H) chain position (45-50,000 mol wt). Comparison of these two bands by two-dimensional isoelectric focusing (2D IEF)-SDS-PAGE and 2D chymotryptic peptide mapping showed no differences indicative of protein dissimilarity. Thus, the two components of the H chain doublet may differ only in a feature of glycosylation that does not affect charge. The two leukemias studied gave only a single band in the H chain position. On 2D peptide mapping and 2D IEF-SDS-PAGE, the patterns for TL of Tlaa and Tlae thymocytes, which are closely related serologically, were broadly similar, but clearly different from the pattern typical of Tlac and Tlad thymocytes. 2D peptide maps of TL from Tlaa thymocytes and Tlaa leukemia cells did not differ. Leukemia cells of Tlab origin (thymocytes TL-) gave 2D peptide and 2D IEF-SDS-PAGE patterns of a third type. With the exception of Tlaa, thymocytes of TL+ mice yielded additional TL products of higher molecular weight than the TL H chain.
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Pietrogrande, Maria Chiara, Nicola Marchetti, Francesco Dondi, and Pier Giorgio Righetti. "Decoding 2D-PAGE complex maps: Relevance to proteomics☆." Journal of Chromatography B 833, no. 1 (March 20, 2006): 51–62. http://dx.doi.org/10.1016/j.jchromb.2005.12.051.

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Griebel, Anja, Christian Obermaier, Reiner Westermeier, Martin Moche, and Knut Büttner. "Fluoreszenzmarkierung von Proteinen für 1D- und 2D-PAGE." BIOspektrum 19, no. 6 (October 2013): 653–55. http://dx.doi.org/10.1007/s12268-013-0375-0.

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Li, Feng, Françoise Seillier-Moiseiwitsch, and Valeriy R. Korostyshevskiy. "Region-based statistical analysis of 2D PAGE images." Computational Statistics & Data Analysis 55, no. 11 (November 2011): 3059–72. http://dx.doi.org/10.1016/j.csda.2011.05.013.

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Sutton, C. A., M. W. Shirley, and M. H. Wisher. "Characterization of coccidial proteins by two-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis." Parasitology 99, no. 2 (October 1989): 175–87. http://dx.doi.org/10.1017/s0031182000058613.

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SummaryTwo dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D SDS–PAGE) has been used to produce ‘fingerprint’ maps of the proteins from each of the 7 species of Eimeria which infect the chicken. All 7 species could be identified from their array of polypeptides but few differences were detected between strains of the same species. Alterations to the polypeptide array associated with the stage of sporulation of the oocysts were observed. lodination of sporozoites, 2D SDS–PAGE, autoradiography and immunoblotting techniques were combined to identify polypeptides with a surface moiety and those which were antigenic.
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Pavlicevic, Milica, Sladjana Stanojevic, and Biljana Vucelic-Radovic. "Influence of extraction method on protein profile of soybeans." Chemical Industry 67, no. 4 (2013): 687–94. http://dx.doi.org/10.2298/hemind120919115p.

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Comparison between protein profiles of soybean obtained by commonly used methods of extraction (Tris buffer and Tris-urea buffer) with methods used for extraction of plant proteins for 2D PAGE analysis (direct solubilization in IEF buffer, acetone extraction, phenol extraction, extraction with urea solubilization buffer and thiourea-urea extraction) was investigated. 2D profiles of samples extracted directly in IEF buffer, in urea solubilization buffer and in acetone were characterized with low number of spots. Analysis of 2D PAGE profiles of Tris buffer and Tris-urea buffer extracts showed high degree of horizontal and vertical streaking. Thiourea-urea extraction gave the highest number of less intense protein spots than phenol extraction. Method of choice, due to a large number of intense spots, would be phenol extraction.
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Tao, R., H. Yamane, H. Sassa, H. Mori, H. Murayama, T. M. Gradziel, A. M. Dandekar, and A. Sugiura. "Stylar Proteins Associated with Gametophytic Self-incompatibility in the Prunus." HortScience 32, no. 3 (June 1997): 514E—514. http://dx.doi.org/10.21273/hortsci.32.3.514e.

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Stylar proteins of four Prunus species, P. avium, P. dulcis, P. mume, and P. salicina, were surveyed by 2D-PAGE combined with immunoblot and N-terminal amino acid sequence analyses to identify S-proteins associated with gametophytic SI in the Prunus. All four S-allelic products tested for P. dulcis could be identified in the highly basic zone of the gel. These S-proteins had Mr of about 28–30 kDa and reacted with the anti-S4-serum prepared from Japanese pear (Pyrus serotina). Two of six S-allelic products tested for P. avium could be also identified in the 2D-PAGE profiles, with roughly the same pI and Mr as those of S-proteins of P. dulcis. Putative S-proteins for P. mume and P. salicina were found in the same area of 2D-PAGE as the area where S-proteins of P. avium and P. dulcis were located. N-terminal amino acid sequence analysis of these proteins revealed that they were similar to S-RNases reported previously.
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Dissertations / Theses on the topic "2D-PAGE"

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Brown, Andrew S. "Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin." Youngstown State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768.

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Queiroz, João Vitor de. "Estratégias bioanalíticas para caracterização de biomarcadores de exposição ao mercúrio em Arapaima gigas e Serrasalmus rhombeus do rio Madeira/bacia amazônica." Botucatu, 2017. http://hdl.handle.net/11449/150276.

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Orientador: Pedro de Magalhães Padilha
Resumo: Este trabalho apresenta os resultados de proteínas associadas ao mercúrio em amostras de tecido muscular e hepático de pirarucu (Arapaima gigas) e piranha preta (Serrasalmus rhombeus) oriundos do complexo hidrelétrico de Jirau, na Bacia do Rio Madeira, região amazônica do Brasil. O proteôma do músculo e fígado dessas espécies foi obtido por eletroforese bidimensional em gel de poliacrilamida (2D PAGE). O mercúrio presente nos spots proteicos foi determinado por espectrometria de absorção atômica em forno de grafite (GFAAS) após mineralização ácida assistida por banho de ultrassom. Os spots proteicos que apresentaram mercúrio foram caracterizados por espectrometria de massas por ionização com eletrospray em sequência (ESI- MS/MS) após digestão tríptica. As determinações GFAAS indicaram que a maior parte do mercúrio está ligada a fração proteica com massa molar (Mm) inferior a 90 kDa. As concentrações de mercúrio nos spots apresentaram-se na faixa de 4,07 – 164,63 µg g-1 no tecido muscular de pirarucu; 0,86 – 25,34 µg g-1 no tecido hepático de pirarucu; 7,67 – 156,18 µg g-1 no tecido muscular de piranha preta e 2,17 – 31,42 µg g-1 no tecido hepático de piranha preta. A análise por ESI-MS/MS permitiu caracterizar em dezenove spots proteicos as seguintes proteínas e/ou enzimas: Triosephosphate isomerase, Fructose-bisphosphate aldolase, Ckmb protein,, Cofilin 2 (Muscle), Actin_ alpha_ cardiac muscle 1a, Actin_ alpha 1_ skeletal muscle, Novel protein similar to zebrafish hemoglobin... (Resumo completo, clicar acesso eletrônico abaixo)
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Cerbino, Maria Rosa. "ABORDAGEM METALOPROTEÔMICA DO MERCÚRIO EM LEITE MATERNO DE COMUNIDADES DA BACIA AMAZÔNICA - BRASIL." Pontifícia Universidade Católica de Goiás, 2016. http://tede2.pucgoias.edu.br:8080/handle/tede/3556.

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Submitted by admin tede (tede@pucgoias.edu.br) on 2016-11-30T18:10:51Z No. of bitstreams: 1 MARIA ROSA CERBINO.pdf: 2627014 bytes, checksum: 98680184bb60e636de01507e3f1eeec4 (MD5)
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Mercury is a potentially toxic element with a wide distribution on the Amazonian environment. This metal is dangerous and responsible for environmental contaminations and human intoxications as it is capable to biomagnifications and bioaccumulate throughout the food chains becoming the main way of exposing the riverine Amazonian communities to methylmercury to whom the main diet is fish. Therefore, studies related to mercury toxicity are of fundamental importance to health and life quality of the Amazonian communities. This study aimed to detect and evaluate possible proteic biomarkers of mercury toxicity in samples of human milk collected in riverine populations of Madeira and Negro rivers in the Brazilian Amazon. Initially total mercury was determined in the hair of breast feeding women to identify who were contaminated with mercury followed by the obtaining of the proteome of milk samples by two-dimensional electrophoresis (2D PAGE) after precipitation of proteins in half acetone. On the proteic spots obtained in the process of protein fractionation of milk samples, detection of mercury was carried out by atomic absorption spectrometry in graphite furnace(GFAAS), where the results showed that mercury was bonded in proteins of molecular weight around 14-26 kDa. The concentration determination of total mercury by GFAAS was also carried out with milk in natura, lyophilized milk and the proteic pellets aiming a mass balance of mercury related to the concentration of this element on milk and pellets. The measurements of mass balance permitted to observe that, in relation to the milk samples from Madeira River, about 85 to 95%of the mercury present in the lyophilized milk is on the proteic fraction. In relation to the breastfeeding women of the Negro River, about 50% of the total mercury is bound in the proteic fraction and the difference of 51% might be bound to the lipidic fraction. However, more studies in this line of research need to be pursued to achieve more robust conclusions.
O mercúrio é um elemento potencialmente tóxico com ampla distribuição no ambiente amazônico. Este metal é perigoso e responsável por contaminações ambientais e intoxicações humanas, já que é capaz de biomagnificar e bioacumular através das cadeias alimentares, tornando-se assim a principal via de exposição às comunidades amazônicas ribeirinhas do metilmercúrio, cuja dieta é baseada em peixes. Sendo assim estudos relacionados à toxicidade do mercúrio são de fundamental importância para a saúde e a qualidade de vida das comunidades amazônicas. Este estudo buscou detectar e avaliar possíveis biomarcadores proteicos da toxicidade do mercúrio em amostras de leite materno coletadas de populações ribeirinhas do rio Madeira e do rio Negro, na Amazônia brasileira. Inicialmente, determinou-se mercúrio total no cabelo das lactantes para identificar quais estavam contaminadas com mercúrio, em seguida obteve-se o proteoma das amostras de leite por eletroforese bidimensional (2D-PAGE) após precipitação das proteínas em meio acetônico. Nos spotsproteicos obtidos no processo de fracionamento das proteínas, nas amostras leite, foram feitas determinações de mercúrio por espectrometria de absorção atômica em forno de grafite (GFAAS), onde os resultados mostraram que o mercúrio se encontra ligado em proteínas de massa molecular na faixa de 14-26 kDa. A determinação da concentração de mercúrio total por GFAAS foi feita também no leite in natura, leite liofilizado e nos pelletsproteicos, com o objetivo de se fazer um balanço de massa de mercúrio em relação à concentração deste elemento no leite e pellets. As medidas de balanço de massa permitiram observar que, em relação às amostras de leite do rio Madeira, cerca de 85 a 95% do mercúrio presente no leite liofilizado encontrase na fração proteica. Em relação às lactantes do rio Negro, cerca de 50% do mercúrio total está ligado na fração proteica e a diferença de 51% pode estar ligado na fração lipídica. Contudo, mais estudos nesta linha de pesquisa devem ser desenvolvidos, para que se possam ter conclusões mais robustas.
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Nadaf, Somayyeh. "Analyse protéomique et transcriptomique de la maturation folliculaire." Thesis, Tours, 2010. http://www.theses.fr/2010TOUR4037.

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La compréhension des mécanismes cellulaires et moléculaires qui sont mis en jeu au moment de la croissance et la maturation pré-ovulatoire induite par la LH, permettra de définir des marqueurs de qualité et de maturité du follicule destiné à ovuler, et ainsi de mieux anticiper le moment de l’ovulation. L’objectif majeur de cette thèse était d’identifier certain des facteurs régulateur impliqués dans la maturation folliculaire par deux approches globales d’analyse protéomique et transcriptomique. La première étude a permis d’établir pour la première fois les cartes protéiques du liquide folliculaire équin et canin. Les analyses comparatives des liquides folliculaires provenant de différents stades physiologiques n’ont montré, dans nos conditions expérimentales, que peu de différences. Nos résultats obtenus dans la deuxième étude indiquent que les différentes méthodes enrichissement du liquide folliculaire peuvent améliorer, pour certaines de manière conséquente, la résolution des gels 2D-PAGE. Notre étude transcriptomique globale a révélé un groupe de gènes différentiellement exprimés dans les cellules folliculaires aux différents stades étudiés. Ces gènes sont potentiellement impliqués pendant le développement folliculaire dans l’espèce équine. Les deux approches (protéomique et transcriptomique) que nous avons utilisées au cours de ce travail sont complémentaires car la connaissance des gènes exprimés par les cellules folliculaires peuvent permettre d’identifier certains gènes codant pour des protéines sécrétoires retrouvées dans le liquide folliculaire
An understanding of the cellular and molecular mechanisms involved in the growth and maturation of the preovulatory follicle induced by LH, will help us to understand and identify the markers of quality and maturity of the follicle destined to ovulate, and better anticipate the time of ovulation. The main objective of this thesis was to identify some regulatory factors involved in follicle maturation using two global approaches: proteomic and transcriptomic analysis. The first study established for the first time the protein map of equine and canine follicular fluids. The comparative analyses of follicular fluid from different physiological stages were shown little or no difference in our experimental conditions. Results obtained with the second study suggested that between depletion and enrichement methods, the enriched follicular fluid can improve for some consistent manner, the resolution of 2D-PAGE gels. Our global transcriptomic study revealed a group of genes differentially expressed in follicular cells at different physiological stages. These genes are potentially involved during follicle development in the equine species. The two approaches (proteomic and transcriptomic) that we used in this work are complementary, as the knowledge of genes expressed by follicle cells can help to identify some genes coding for secretory proteins secreted in follicular fluid
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Fulton, Benjamin L. "2D-PAGE Analysis of Myocardial Collagen in Male and Female Spontaneously Hypertensive Rats." Connect to resource online, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1219668882.

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Schmitz, Gabriela Justamante Handel. "Análise comparativa dos proteomas das raízes tuberosas de mandioca (Manihot esculenta Crantz) de variedades de mesa e indústria." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-28082015-154641/.

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A mandioca (Manihot esculenta Crantz) é uma das principais culturas do mundo, havendo grande variabilidade genética. As variedades são classificadas com base na palatabilidade e toxicidade das raízes, em mansas ou doces e bravas ou amargas. Apesar da importância, o potencial da mandioca é pouco explorado, não sendo conhecidos, em nível molecular, os elementos determinantes para as suas características. Assim, pretendeu-se identificar, empregando a 2D-PAGE, proteínas que possam estar associadas com as diferenças físico-químicas das raízes tuberosas de variedades de mesa (IAC 576-70 e IAC 06-01), indústria (Cigana Preta, IAC 12 e IAC 90) e de uso misto (Vassourinha Paulista). Após extração de proteínas e separação por 2D-PAGE, as imagens dos géis foram analisadas no programa Delta2D (DECODON), sendo realizada análise estatística utilizando-se ANOVA (p<0,01), Heat Map e Análises de Componentes Principais (ACP) e de Agrupamentos. Os 146 spots de interesse foram removidos dos géis e suas proteínas digeridas e sequenciadas por espectrometria de massas. Algumas proteínas refletiram as características fenotípicas das variedades em estudo, especialmente entre as de mesa e indústria. Pela ACP, foram explicados 54,54% da variabilidade entre as amostras. A primeira componente separou as variedades exclusivamente de mesa de todas as demais, enquanto a segunda separou a IAC 90 de todas as outras, sendo esta caracterizada por um perfil proteico diferente das demais amostras de uso industrial. A IAC 576-70 e a IAC 12 apresentaram alta correlação positiva, assim como, a Vassourinha e a Cigana. A Análise de Agrupamentos corroborou as informações da ACP, revelando que o proteoma das raízes tuberosas refletiu diferenças fenotípicas entre as variedades.
Cassava (Manihot esculenta Crantz) is a main crop with large genetic variability. The varieties are classified according palatability and toxicity of the roots as sweet or bitter cassavas. Despite its importance, little is known about the molecular basis of phenotypic characteristics. Therefore, this study aimed to identify proteins associated to the differences between the sweet (\'IAC 576-70\' e \'IAC 06-01\'), bitter (\'Cigana Preta\', \'IAC 12\' e \'IAC 90\') and the mixed-use (\'Vassourinha Paulista\') varieties by 2D-PAGE. After the protein extraction and separation by 2D-PAGE, the gel images were analyzed through the software Delta 2D (DECODON), and the statistical analysis were performed with ANOVA (p<0,01), Heat Map, Principal Component Analysis (PCA) and Cluster Analysis. The 146 significant spots were removed from the gels, digested and sequenced by mass spectrometry. Some proteins were related to the physico-chemical characteristics of the varieties, especially between the sweet and the bitter. Variability of the samples was explained at the level of 54,54% by the PCA. The first component separated the sweet varieties from all others while the second one separated the \'IAC 90\' from all others. This variety was characterized by a different protein profile among the bitter cassavas. The \'IAC 576-70\' and the \'IAC 12\' were positively correlated, as well as, \'Vassourinha\' and the \'Cigana\'. Cluster Analysis agreed the PCA information, revealing that the proteomes of the tuberous roots reflected phenotypic differences among the varieties.
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MILLI, Alberto. "2D-page coupled to mass spectrometry for proteomic analysis of human, microbial and plant samples." Doctoral thesis, Università degli Studi di Verona, 2009. http://hdl.handle.net/11562/337513.

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Il progetto di dottorato è stato condotto nel Laboratorio di Proteomica del Dipartimento di Biotecnologie dell’Università di Verona. Per la stesura di tale progetto sono state instaurate collaborazioni con alcuni laboratori, sia interni allo stesso Dipartimento sia appartenenti ad altre Università o Istituti di Ricerca. In particolare, per quanto riguarda lo studio su cellule di cancro colorettale trattate con un nuovo chemiofarmaco, il Laboratorio del Prof. Zunino (Fondazione IRCCS, Istituto Nazionale dei Tumori, Milano) ha fornito tutti i campioni biologici, mentre il gruppo del Prof. Marengo (Dipartimento di Scienze dell’Ambiente e della Vita, Università degli Studi del Piemonte Orientale, Alessandria) ha effettuato l’analisi statistica multivariata dei dati. Per quanto riguarda invece la caratterizzazione microbiologica, biochimica e proteomica del ceppo tannasi-positivo VP08 di Lactobacillus plantarum, il lavoro è stato condotto in collaborazione con il Dottor Zapparoli (Laboratorio di Microbiologia, Dipartimento di Biotecnologie, Università di Verona). L’indagine proteomica su campioni di foglie di Vitis vinifera suscettibile a Plasmopara viticola è stata realizzata in collaborazione col gruppo della Dottoressa Polverari (Laboratorio di Biotecnologie Fitopatologiche, Dipartimento di Biotecnologie, Università di Verona). Infine, il gruppo del Prof. Zolla (Laboratorio di Proteomica, Dipartimento di Scienze Ambientali, Università degli Studi della Tuscia, Viterbo) ha effettuato le analisi di spettrometria di massa per l’identificazione delle proteine di interesse individuate nei diversi studi. Ulteriori analisi di massa sono state svolte, in parte, presso il medesimo Laboratorio di Proteomica del Dipartimento di Biotecnologie dell’Università di Verona e, in parte, presso il Laboratorio di Proteomica del Dottor Vindigni (Centro Internazionale di Ingegneria Genetica e Biotecnologia “ICGEB”, Trieste). In questo progetto di dottorato sono stati applicati i metodi classici della proteomica comparativa basata sull’elettroforesi bidimensionale per l’analisi di campioni umani, microbici e vegetali legati a tre differenti problematiche, riguardanti rispettivamente: 1) la risposta di una linea cellulare di cancro al colon-retto ad un nuovo tipo di inibitore delle istone deacetilasi 2) l’effetto dell’acido tannico sul microrganismo del vino Lactobacillus plantarum in condizioni di carenza di nutrienti 3) i cambiamenti nel proteoma di foglia di Vitis vinifera cultivar Pinot Noir a diversi tempi dopo infezione con Plasmopara viticola
The proteome of a cell or an organelle provides information about the ensemble of proteins and protein isoforms expressed in that cell or organelle under specific physiological conditions and at a specific time. Proteomic approaches provide several novel possibilities to address biological questions. In fact, the large-scale screening approach of proteomics enables protein expression studies that are impossible to perform using classical molecular biology and biochemical techniques, in which the expression of only one or a few proteins is studied at a time. Instead, proteomic techniques allow for the analysis of up to thousands of proteins simultaneously, in any tissue or organelle, under any given physiological condition. Thus, proteomic applications are growing in many areas of research and proteomic approaches are nowadays widely exploited for cancer, microbial, and plant investigations. The present work was focused on three proteomics studies: 1) evaluation of the cell response to a novel histone deacetylase inhibitor in colon cancer cell 2) effect of tannic acid on lactobacillus plantarum wine strain during starvation 3) analysis of grapevine leaves after Plasmopara viticola infection The thesis work was conducted at the Proteomics Laboratory of the Biotechnology Department of the University of Verona, in collaboration with other laboratories: concerning the study on colorectal cancer cells, the Laboratory of Oncology of the IRCCS Foundation “Istituto Nazionale dei Tumori”, Milan, provided all the biological samples, whilst the multivariate analysis of protein profiles was possible thanks to the collaboration with the Department of Environmental and Life Sciences of the University of Eastern Piedmont, Alessandria. The biochemical and proteomic analysis of lactobacillus plantarum wine strain was the result of the collaboration with laboratory of Dr. Zapparoli (Department of Biotechnology of the University of Verona). Proteomic investigations on the Grapevine leaves infected by Plasmopara viticola were performed in collaboration with laboratory of Dr. Polverari (Department of Biotechnology of the University of Verona). Finally, the identification of proteins for all the proteomic analyses performed were possible thanks to the collaboration with the Proteomics laboratories of Department of Environmental Sciences, Tuscia University, Viterbo, and of International Centre for Genetic Engineering and Biotechnology, Trieste. The results thus obtained are here discussed and evaluated.
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Vieira, José Cavalcante Souza. "Investigação de metalotioneínas em peixes da região de Jirau - bacia do Rio Madeira - Rondônia." Botucatu, 2017. http://hdl.handle.net/11449/150736.

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Orientador: Pedro de Magalhães Padilha
Resumo: Devido a sua grande concentração de nutrientes, tais como proteínas, vitaminas e minerais, o peixe é considerado um dos alimentos mais saudáveis que se pode encontrar na natureza. No entanto, a ingestão de peixes é considerada a forma predominante de via de exposição do ser humano ao mercúrio (Hg), principalmente para as populações que vivem às margens dos rios, onde o peixe constitui a principal fonte de proteína. Na tentativa de elucidar os mecanismos de toxicidade das espécies mercuriais, o teor desse metal tem sido estudado intensamente pela comunidade científica nas últimas décadas em amostras de solo, sedimentos, humanos e peixes na Amazônia brasileira. Sabe-se que as espécies mercuriais bioacumuladas nos tecidos dos seres vivos ligam-se a metaloproteínas, e quando há uma concentração alta de metal tóxico nos organismos, esses passam a expressar proteínas de defesa, denominadas metalotioneínas (MTs) responsáveis pelo transporte e eliminação de metais tóxicos. Apesar de estudos mostrarem o aumento das metalotioneínas em animais expostos a metais potencialmente tóxicos, essas proteínas não foram caracterizadas para confirmação de sua veridicidade, são analisadas por métodos indiretos, esse fato leva a necessidade de técnicas mais precisas na identificação de metalotioneínas. Levando em consideração o exposto esse estudo teve como objetivo otimizar métodos de quantificação de mercúrio e técnica de eletroforese para identificação de possíveis metalotioneínas biomarcadoras d... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Due to its high concentration of nutrients, such as proteins, vitamins and minerals, fish is considered one of the healthiest foods that one can find in nature. However, fish intake is considered to be the predominant human exposure pathway to mercury (Hg), especially for populations living along riverbanks where fish are the main source of protein. In the attempt to elucidate the toxicity mechanisms of mercurial species, the content of this metal has been intensively studied by the scientific community in recent decades in soil, sediment, human and fish samples in the Brazilian Amazon. It is known that mercurial species bioaccumulated in the tissues of living beings bind to metalloproteins, and when there is a high concentration of toxic metal in organisms, they begin to express defense proteins, called metallothioneins (MTs) responsible for the transport and elimination of Toxic metals. Although studies have shown the increase of metallothioneins in animals exposed to potentially toxic metals, these proteins have not been characterized to confirm their veridicity, are analyzed by indirect methods, this fact leads to the need for more precise techniques in the identification of metallothioneins. Taking into account the above, this study aimed to optimize mercury quantification methods and electrophoresis technique for identification of possible mercury biomarkers metallothionein in muscular and hepatic tissue of fish of economic interest, Tucunaré (Cichla spp.), Filhote (Bra... (Complete abstract click electronic access below)
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9

Lonardoni, Francesco. "Discovery and quantification of proteins of biological relevance through differential proteomics and biosensing." Thesis, Cranfield University, 2012. http://dspace.lib.cranfield.ac.uk/handle/1826/8676.

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Medical diagnosis is the process of attempting to determine and/or identify a possible disease or disorder. This process is revealed by biomarkers, defined by The Food and Drug Administration (FDA) as “characteristics that are objectively measured and evaluated as indicators of normal biologic processes, pathogenic processes, or pharmacologic responses to a therapeutic intervention”. The process of biomarker discovery has been boosted in the last years by proteomics, a research discipline that takes a snapshot of the entire wealth of proteins in an organism/ tissue/ cell/ body fluid. An implementation of the analysis methods can help in isolate proteins present in the low range of concentrations, such as biomarkers very often are. An established biomarker can further be measured with the help of biosensors, devices that can be employed in the point-of care diagnostics. This PhD thesis shows and discusses the results of three projects in the field of protein biomarkers discovery and quantification. The first project exploited proteomics techniques to find relevant protein markers for Intrauterine Growth Restriction (IUGR) in cordonal blood serum (UCS) and amniotic fluid (AF). A 14 proteins in UCS and 11 in AF were successfully identified and found to be differentially expressed. Molecularly Imprinted Polymers (MIPs) directed towards proteins and peptides containing phosphotyrosine were then produced, with the final goal of selectively extracting phosphopeptides from a peptide mixture. An alteration of the phosphorylation pattern is in fact often associated to important diseases such as cancer. The polymers were produced as nanoparticles, that were characterised with Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM). A recipe was also tested for binding capacity towards phosphotyrosine. A Surface Plasmon Resonance (SPR) biosensor to quantify hepcidin hormone was finally produced. This is the major subject in iron homeostasis in vertebrates and marker of iron unbalance diseases. A calibration curve was made and affinity/kinetic parameters for the ligand employed were measured.
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Mabiya, Thembeka. "Development of a plum chromosome doubling method and proteomics and biochemical characterization." University of the Western Cape, 2015. http://hdl.handle.net/11394/4875.

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>Magister Scientiae - MSc
Chromosome doubling has become an important tool in breeding programmes as it offers the ability of introducing novel traits into existing plants. Doubled haploid plants are highly valued by both consumers and breeders as these plants usually show larger flower, leaves and fruit, thus making them more marketable. Marianna open pollinated plum rootstocks’ adaptability to different soil types and moisture conditions has been favoured in polyploidy studies as parental material in breeding programmes. The potential of the microtubule depolymerizing herbicide (oryzalin) for in vitro chromosome doubling were investigated by optimizing the concentration and incubation time of plant shoots to the antimitotic agent. Meristem tissues were treated for two time intervals (24 and 48 h) with five different concentrations of oryzalin (50, 75, 100, 150 or 200 μM) in liquid Murashige and Skoog (MS) medium. After treatment, plants were allowed to grow under a 16/8 h light/dark photoperiod at 24±2˚C for 4 weeks. One and two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) was used to separate, visualise and identify differently expressed proteins. Furthermore, changes in ROS accumulation, photosynthetic pigmentation, lipid peroxidation and antioxidant enzyme activity (SOD, APX and GR) were investigated. Flow cytometry results revealed that treatment of plants with oryzalin concentrations ranging from 75 to 150 μM induced ploidy after 24 h exposure whereas, 200 μM produced mixoploids containing both tetraploid and octoploids plants after 24 h exposure. Longer incubations of 48 h were detrimental to plant tissues as complete mortality was observed in the higher concentration (100 to 200 μM) treatments. Mass spectrometry analysis identified 14 differentially expressed protein spots that were characterized into different functional categories. ROS accumulation, the extent of lipid peroxidation and antioxidant capacity were differentially regulated in response to oryzalin treatment whereas photosynthetic pigments were significantly enhanced. The results suggests that oryzalin-induced proteins may act as potential biomarkers to improve fruit characteristics in future breeding programs whereas antioxidant enzymes play an important role in scavenging ROS in plants to enhance their adaptability to different environmental conditions.
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Books on the topic "2D-PAGE"

1

Posch, Anton, ed. 2D PAGE: Sample Preparation and Fractionation. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-064-9.

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Posch, Anton, ed. 2D PAGE: Sample Preparation and Fractionation. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-210-0.

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Anton, Posch, ed. 2D PAGE: Sample preparation and fractionation. Totowa, NJ: Humana Press, 2008.

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Reichert, Gerd Hermann. Autosomale Trisomien bei Maus und Mensch, Untersuchung der Proteinmuster durch IEF-2D-PAGE [IEF-D-PAGE]. [S.l.]: [s.n.], 1985.

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Posch, Anton. 2D PAGE : Sample Preparation and Fractionation: Volume 1. Humana Press, 2010.

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Posch, Anton. 2D PAGE : Sample Preparation and Fractionation: Volume 2. Humana Press, 2010.

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2D PAGE: Sample Preparation and Fractionation: Volume 1 (Methods in Molecular Biology). Humana Press, 2008.

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Posch, Anton. 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology). Humana Press, 2008.

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Book chapters on the topic "2D-PAGE"

1

Marengo, Emilio, Elisa Robotti, and Marco Bobba. "2D-PAGE Maps Analysis." In Methods in Molecular Biology™, 291–325. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-117-8_16.

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Robotti, Elisa, Elisa Calà, and Emilio Marengo. "for 2D-PAGE Data Analysis." In Methods in Molecular Biology, 15–31. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1641-3_2.

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May, Caroline, Frederic Brosseron, Kathy Pfeiffer, Helmut E. Meyer, and Katrin Marcus. "Proteome Analysis with Classical 2D-PAGE." In Methods in Molecular Biology, 37–46. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-885-6_3.

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May, Caroline, Frederic Brosseron, Kathy Pfeiffer, Kristin Fuchs, Helmut E. Meyer, Barbara Sitek, and Katrin Marcus. "Proteome Analysis with Classical 2D-PAGE." In Methods in Molecular Biology, 53–62. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1024-4_5.

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Maaß, Sandra. "Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE." In Methods in Molecular Biology, 141–62. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8695-8_11.

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Le, Kim Phuong Uyen, Phuong Uyen Vo, Kieu Minh Le, Thi Thu Hien Pham, Huu Hung Nguyen, Dang Giap Do, Minh Thong Tran, Ngoc Phuc Chau Do, and Thi Thu Hoai Nguyen. "2D-Page Analysis of Vietnamese Colorectal Cancer Tissue Samples." In 6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6), 287–93. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-4361-1_48.

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Bitrián, Marta, Antonio F. Tiburcio, and Rubén Alcázar. "Determination of Posttranslational Modifications by 2D PAGE: Applications to Polyamines." In Methods in Molecular Biology, 337–41. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7398-9_28.

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Álvaro, Francisco, Francisco Cruz, Joan-Andreu Sánchez, Oriol Ramos Terrades, and José-Miguel Benedí. "Page Segmentation of Structured Documents Using 2D Stochastic Context-Free Grammars." In Pattern Recognition and Image Analysis, 133–40. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-38628-2_15.

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Green, George R., and Duc P. Do. "Purification and Analysis of Variant and Modified Histones Using 2D PAGE." In The Nucleus, 285–302. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-461-6_16.

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Marengo, Emilio, and Elisa Robotti. "A New Algorithm for the Simulation of SDS 2D-PAGE Datasets." In Methods in Molecular Biology, 407–25. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-821-4_34.

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Conference papers on the topic "2D-PAGE"

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Wang, Mark M., Frederick B. McCormick, and Sadik C. Esener. "2D and 3D Equalizers for Page-Oriented Optical Memories." In Optics in Computing. Washington, D.C.: Optica Publishing Group, 1997. http://dx.doi.org/10.1364/oc.1997.owd.4.

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In this study we investigate the use of 2D and 3D equalizers to suppress intersymbol interference (ISI) in 2D parallel channels. The techniques demonstrated here are best suited for parallel implementation and can be used for any page-oriented readout application including increasing the data density achievable in volume optical memories or increasing the number of links achievable in free-space optical interconnects. Page-oriented optical memories are currently being investigated for their potential as high data-rate, high capacity storage devices,1-3 however the cost-effectiveness of such devices is limited by their requirements for high resolution in the optical system and severe alignment tolerances on the 2D detector array. Various techniques have been used in an attempt to reduce the amount of ISI generated by optical aberrations and misalignment including the use of guard bands around each bit and also reduced fill-factor detectors,2,3 however such techniques have the undesirable result of also reducing the signal intensity of each bit. An alternative approach is to remove the crosstalk electronically after detection. Serial equalizers have been used for years in communications and storage channels as a method of reducing ISI in a temporal bit stream.4 Limited 2D equalizers have also been demonstrated to reduce inter-track interference in high density optical disc recordings with multiple-beam readout heads.5-7 The use of partial-response maximum-likelihood (PRML) detection has been demonstrated to reduce the effects of crosstalk in page-oriented memories, however the complexity of PRML detectors increases rapidly as the number of spatial dimensions and the number of possible states increases.8 In this paper we propose the design of 2D and 3D equalizers suitable for page-access optical memories and investigate the performance of both linear and decision feedback implementations.
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"Page Analysis by 2D Conditional Random Fields." In International Conference on Pattern Recognition Applications and Methods. SciTePress - Science and and Technology Publications, 2013. http://dx.doi.org/10.5220/0004266505640567.

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Neifeld, Mark A. "2D Coding and Signal Processing for Volume Memory Interfaces." In Optics in Computing. Washington, D.C.: Optica Publishing Group, 1997. http://dx.doi.org/10.1364/oc.1997.owd.1.

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Volume optical memory represents a unique type of storage channel. It can be characterized as a 2D parallel, space-variant system with potentially time-varying characteristics. Like any other storage channel however, imperfections and noise degrade the SNR of retrieved data and this degradation may result in data errors. As with traditional memory and communications systems, detection and error handling techniques can be used to recover lost SNR and correct data errors.[1,2] The success of detection theoretic techniques is based on the use of prior knowledge concerning channel characteristics and noise correlations, while error correction techniques are based on the addition of structured redundancy to the stored data. The 2D nature of the volume optical storage channel requires that detection and error correction techniques utilize prior knowledge of the 2D noise and error correlations to produce efficient solutions, thus 2D techniques for detection and error correction are required. In addition, such 2D parallel algorithms will admit 2D parallel implementations which serve to avoid critical data bottlenecks that can arise upon serialization. In this paper we will describe the use of a parallel 2D detection algorithm and a 2D interleaving technique that can improve data fidelity and increase memory capacity in the case of page access volume memory.
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Latib, Norhidayu Abdul, Safida Anira Norshaha, Gires Usup, and Nurul Yuziana Mohd Yusof. "2D-PAGE protein analysis of dinoflagellate Alexandrium minutum based on three different temperatures." In THE 2015 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4931248.

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"2D-PAGE Texture Classification using Support Vector Machines and Genetic Algorithms - An Hybrid Approach for Texture Image Analysis." In International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2013. http://dx.doi.org/10.5220/0004187400050014.

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6

Chen, C. H., B. Hoanca, C. B. Kuznia, J. M. Wu, and A. A. Sawchuk. "Smart Pixel Array Network Interface (SAPIENT) for 2D Parallel Data Packet Networks." In Optics in Computing. Washington, D.C.: Optica Publishing Group, 1997. http://dx.doi.org/10.1364/oc.1997.othd.11.

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We describe a SmArt PIxEl for NeTwork Interface (SAPIENT) chip that performs interfacing of processors to 2-D parallel optical free space networks. This network transfers 2-D parallel data packets between processors on digital optical channels. We assume these parallel data packets contain address information for the destination processor, similar to the format of an ATM packet network, except the packets are passed between processors in a parallel format, in a single clock cycle. Each SAPIENT chip is capable of checking the address bits of an incoming parallel data packet, re-transmitting (or downloading) the packet, and loading electronic data onto the optical network from its host processor. Each data packet arrives as a 2D page-wide (9 bits) packet of network data and can be easily scaled up to larger N × N packets. The SAPIENT demonstration chip contains a 3 × 3 array of smart pixels that provide optical detection and transmission. The SAPIENT also contains address detection and contention avoidance circuitry. In this paper, we describe the optoelectronic technology involved and the circuit function of the SAPIENT chip. We describe a SAPIENT chip in a multiple processor network and present simulation of the SAPIENT interface.
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Evjenth, Andreas, Otto Andreas Moe, Iselin Violet Kjelland Schøn, and Thomas J. Impelluso. "A Dynamic Model for Motion of an ROV due to On-Board Robotics." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-70110.

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A new method in dynamics — the Moving Frame Method (MFM) — is used to conduct the analysis of how a robotic appendage (manipulator) on a Remotely Operated Vehicle (ROV) affects the motion of the ROV. An ROV performs multiple tasks on the seabed in the oil service industry. In most cases, an ROV pilot monitors and adjusts the movement of the vehicle due to induced motion by currents, buoyancy and the manipulators. Simulation data would assist the pilot and improve the stability of the ROV. This paper exploits a new method to analyze the induced movements of the ROV. The method uses the Special Euclidean Group (SE(3)) and the MFM. The method is supplemented with a restricted variation on the angular velocity to extract the equations of motion for the ROV. Then the equations of motion are solved numerically using Runge-Kutta Method and a reconstruction formula (founded upon the Cayley-Hamilton theorem) to secure the 3D rotations of the vehicle. The resulting motion is visualized with selected 2D plots. The 3D animation is displayed on a 3D web page. This paper closes with a summary of the simplifications used in the model and suggestions for advanced work.
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Devanathan, Srikanth, and Karthik Ramani. "Towards Enabling Visual Design Exploration Involving Multiple Abstractions of Design Descriptions." In ASME 2011 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/detc2011-47291.

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Designers use several visual tools for exploring and understanding design problems and solutions. House of Quality (HoQ), function-structure, Morphological matrices, concept selection tables, 2D drawings are some of the visual tools and representations used in mechanical design. In this article we attempt to connect these visual tools and their underlying models to support exploration in early design using a representation called the working knowledge model (WKM). We identify two key aspects in design that are important for establishing such connections: different abstractions are used to describe the same design element, and several alternatives are considered during exploration. The constituent elements of the visual tools such as engineering characteristics (ECs) in a HoQ are described using classes of the information model. A simple wiki-based implementation is described that allows the user to tag the wiki text with WKM classes, which is then extracted to populate a database. This information is used by visual tools that can be embedded within a wiki page; the decisions taken by the user using these visual tools are then incorporated back into the WKM database and the wiki is updated if needed. A case study of the design of an automotive flow control valve is described to demonstrate the prototype.
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Reports on the topic "2D-PAGE"

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Michalski, A,, D. Andersson, R. Rossi, and C. Soriano. D7.1 DELIVERY OF GEOMETRY AND COMPUTATIONAL MODEL. Scipedia, 2021. http://dx.doi.org/10.23967/exaqute.2021.2.020.

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This document describes the industrial application, on which the developments of the project are implemented, and the CFD set-up. The developments are implemented over six analysis cases with increasing complexity starting from a 2D geometry with mean wind inflow to a 3D geometry with turbulent inflow and real-time shape optimization. The application represents the CAARC tall building model, which has served as a benchmark model for many studies since the 1970’s when it was first developed. Base moments (bending and torsional moments) of the building are extracted for validation by comparison of the results with the benchmark study. Page 3 of 19 Deliverable 7.1
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Badia, S., A. Martín, J. Principe, C. Soriano, and R. Rossi. D3.1 Report on nonlinear domain decomposition preconditioners and release of the solvers. Scipedia, 2021. http://dx.doi.org/10.23967/exaqute.2021.2.021.

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This document describes the industrial application, on which the developments of the project are implemented, and the CFD set-up. The developments are implemented over six analysis cases with increasing complexity starting from a 2D geometry with mean wind inflow to a 3D geometry with turbulent inflow and real-time shape optimization. The application represents the CAARC tall building model, which has served as a benchmark model for many studies since the 1970’s when it was first developed. Base moments (bending and torsional moments) of the building are extracted for validation by comparison of the results with the benchmark study. Page 3 of 19 Deliverable 7.1
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