Journal articles: '2D MS/MS'

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Delahunty, Claire, and John R. Yates III. "Protein identification using 2D-LC-MS/MS." Methods 35, no. 3 (March 2005): 248–55. http://dx.doi.org/10.1016/j.ymeth.2004.08.016.

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Ketha, Hemamalini, Rajiv Kumar, and Ravinder J. Singh. "LC-MS/MS for Identifying Patients with CYP24A1 Mutations." Clinical Chemistry 62, no. 1 (January 1, 2016): 236–42. http://dx.doi.org/10.1373/clinchem.2015.244459.

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Abstract BACKGROUND Patients have been described with loss-of-function CYP24A1 (cytochrome P450, family 24, subfamily A, polypeptide 1) mutations that cause a high ratio of 25-hydroxyvitamin D to 24,25-dihydroxyvitamin D [25(OH)D/24,25(OH)2D], increased serum 1,25-dihydroxyvitamin D, and resulting hypercalcemia, hypercalciuria and nephrolithiasis. A 25(OH)D/24,25(OH)2D ratio that can identify patients who are candidates for confirmatory CYP24A1 genetic testing would be valuable. We validated an LC-MS/MS assay for 24,25(OH)2D (D3 and D2) and determined a 25(OH)D/24,25(OH)2D cutoff to identify candidates for confirmatory genetic testing. METHODS After addition of isotope-labeled internal standard, serum samples were extracted by solid-phase extraction, derivatized with 4-phenyl-1,2,4,-triazoline-3,5-dione, and quantified by LC-MS/MS. We measured 25(OH)D/24,25(OH)2D in 91 healthy patients and 34 patients with clinically suspected CYP24A1-mediated hypercalcemia. RESULTS The limits of detection and quantification were 0.03 (0.2) and 0.1 (0.24) nmol/L, respectively, for 24,25(OH)2D3, and 0.1 (0.23) and 0.5 (1.16) nmol/L for 24,25(OH)2D2. Intra- and interassay imprecision was 4%–15% across the analytical measurement range of 0.1–25 ng/mL (0.2–60 nmol/L). No interference was observed with 25(OH)D and 1,25(OH)2D. 25(OH)D/24,25(OH)2D of 7–35 was observed in healthy patients, whereas in 2 patients with CYP24A1 mutations, 25(OH)D/24,25(OH)2D was significantly increased (99–467; P < 0.001). A 25(OH)D/24,25(OH)2D ratio ≥99 identified patients who were candidates for CYP24A1 genetic testing. CONCLUSIONS Increased 25(OH)D/24,25(OH)2D supports the diagnosis of reduced CYP24A1 activity due to mutations in CYP24A1. Measurement of 25(OH)D/24,25(OH)2D should be considered a part of the clinical workup in patients with hypercalcemia of otherwise unknown etiology.

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Escoffier, Patricia, Luc Paris, Bahram Bodaghi, Martin Danis, Dominique Mazier, and Carine Marinach-Patrice. "Pooling Aqueous Humor Samples: Bias in 2D-LC-MS/MS Strategy?" Journal of Proteome Research 9, no. 2 (February 5, 2010): 789–97. http://dx.doi.org/10.1021/pr9006602.

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Arrigoni, Giorgio, Celine Fernandez, Cecilia Holm, Michaela Scigelova, and Peter James. "Comparison of MS/MS Methods for Protein Identification from 2D-PAGE." Journal of Proteome Research 5, no. 9 (September 2006): 2294–300. http://dx.doi.org/10.1021/pr0601281.

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Zhang, Lina, Chih-Wei Liu, and Qibin Zhang. "Online 2D-LC-MS/MS Platform for Analysis of Glycated Proteome." Analytical Chemistry 90, no. 2 (December 27, 2017): 1081–86. http://dx.doi.org/10.1021/acs.analchem.7b03342.

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Xiao, Mingming, Yajing Chen, Huan Yu, Su Wei, Kang Yu, Huan Zhao, and Ruibing Chen. "Analysis of the whole serum proteome using an integrated 2D LC-MS/MS system." Anal. Methods 6, no. 18 (2014): 7157–60. http://dx.doi.org/10.1039/c4ay01274g.

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Xu, Jiyu, Shuxin Zheng, Mimi Li, Xiaoyan Liu, Haidan Sun, Zhengguang Guo, Jing Wei, Lulu Jia, and Wei Sun. "A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome." Diagnostics 12, no. 9 (September 9, 2022): 2184. http://dx.doi.org/10.3390/diagnostics12092184.

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Profiling bodily fluids is crucial for monitoring and discovering metabolic markers of disease. In this study, a comprehensive analysis approach based on 1D-LC-MS/MS and 2D-LC-MS/MS was applied to profile normal human urine metabolites from 348 children and 315 adults. A total of 2357 metabolites were identified, including 1831 endogenous metabolites and 526 exogenous ones. In total, 1005 metabolites were identified in urine for the first time. The urinary metabolites were mainly involved in amino acid metabolism, small molecule biochemistry, lipid metabolism and cellular compromise. The comparison of adult’s and children’s urine metabolomes showed adults urine had more metabolites involved in immune response than children’s, but the function of binding of melatonin, which belongs to the endocrine system, showed a higher expression in children. The urine metabolites detected by the 1D-LC-MS/MS method were mainly related to amino acid metabolism and lipid metabolism, and the 2D-LC-MS/MS method not only explored metabolites from 1D-LC-MS/MS but also metabolites related to cell signaling, cell function and maintenance, etc. Our analysis comprehensively profiled and functionally annotated the metabolome of normal human urine, which would benefit the application of urinary metabolome to clinical research.

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Rao, R. Nageswara, R. Mastan Vali, and Dhananjay D. Shinde. "On-line 2D-LC-ESI/MS/MS determination of rifaximin in rat serum." Biomedical Chromatography 23, no. 11 (November 2009): 1145–50. http://dx.doi.org/10.1002/bmc.1236.

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Lenglois, S., M. Moser, and A. O. A. Miller. "Microsupport with Two-Dimensional Geometry (2D-MS)." Cytotechnology 44, no. 1/2 (2004): 47–54. http://dx.doi.org/10.1023/b:cyto.0000043403.20008.02.

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Bekkach, Yasin, Annemieke C. Heijboer, Erik Endert, and Mariëtte T. Ackermans. "Determination of urinary aldosterone using a plasma aldosterone 2D ID LC–MS/MS method." Bioanalysis 8, no. 17 (September 2016): 1765–75. http://dx.doi.org/10.4155/bio-2016-0115.

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Kalyanaraman, L., K. K. Sree Ganesh, R. Mohan Kumar, R. Pichai, and K. Vyas. "CHARACTERIZATION OF A BIOACTIVE DERIVATIVE OF CALOPHYLLOLIDE BY 2D NMR AND LC-MS/MS." Journal of Liquid Chromatography & Related Technologies 37, no. 8 (February 10, 2014): 1087–93. http://dx.doi.org/10.1080/10826076.2013.765463.

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Mella, Malorie, Brendan Schweitzer, Claude R. Mallet, Tara Moore, and Sabra Botch-Jones. "Detection of Cocaine and Metabolites in Bone Following Decomposition Using 2D LC–MS-MS." Journal of Analytical Toxicology 42, no. 4 (December 28, 2017): 265–75. http://dx.doi.org/10.1093/jat/bkx106.

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Golizeh, Makan, André LeBlanc, and Lekha Sleno. "Identification of Acetaminophen Adducts of Rat Liver Microsomal Proteins using 2D-LC-MS/MS." Chemical Research in Toxicology 28, no. 11 (November 9, 2015): 2142–50. http://dx.doi.org/10.1021/acs.chemrestox.5b00317.

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Luo, Mi, Liyun Zheng, Suwei Wang, Jianghua Zhu, Zhuohong Tang, and Pingming Gao. "Characterization of four major degradation products in metformin by 2D LC-QTOF/MS/MS." Journal of Pharmaceutical and Biomedical Analysis 192 (January 2021): 113662. http://dx.doi.org/10.1016/j.jpba.2020.113662.

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Sinclair, John, Gergana Metodieva, Dimitra Dafou, Simon A. Gayther, and John F. Timms. "Profiling signatures of ovarian cancer tumour suppression using 2D-DIGE and 2D-LC-MS/MS with tandem mass tagging." Journal of Proteomics 74, no. 4 (April 2011): 451–65. http://dx.doi.org/10.1016/j.jprot.2010.12.009.

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Vlot, Mariska C., Laura Boekel, Jolijn Kragt, Joep Killestein, Barbara M. van Amerongen, Robert de Jonge, Martin den Heijer, and Annemieke C. Heijboer. "Multiple Sclerosis Patients Show Lower Bioavailable 25(OH)D and 1,25(OH)2D, but No Difference in Ratio of 25(OH)D/24,25(OH)2D and FGF23 Concentrations." Nutrients 11, no. 11 (November 15, 2019): 2774. http://dx.doi.org/10.3390/nu11112774.

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Vitamin D (VitD) insufficiency is common in multiple sclerosis (MS). VitD has possible anti-inflammatory effects on the immune system. The ratio between VitD metabolites in MS patients and the severity of the disease are suggested to be related. However, the exact effect of the bone-derived hormone fibroblast-growth-factor-23 (FGF23) and VitD binding protein (VDBP) on this ratio is not fully elucidated yet. Therefore, the aim is to study differences in total, free, and bioavailable VD metabolites and FGF23 between MS patients and healthy controls (HCs). FGF23, vitD (25(OH)D), active vitD (1,25(OH)2D), inactive 24,25(OH)D, and VDBP were measured in 91 MS patients and 92 HCs. Bioavailable and free concentrations were calculated. No difference in FGF23 (p = 0.65) and 25(OH)D/24.25(OH)2D ratio (p = 0.21) between MS patients and HCs was observed. Bioavailable 25(OH)D and bioavailable 1.25(OH)2D were lower (p < 0.01), while VDBP concentrations were higher in MS patients (p = 0.02) compared with HCs, specifically in male MS patients (p = 0.01). In conclusion, FGF23 and 25(OH)D/24.25(OH)2D did not differ between MS patients and HCs, yet bioavailable VitD concentrations are of potential clinical relevance in MS patients. The possible immunomodulating role of VDBP and gender-related differences in the VD-FGF23 axis in MS need further study.

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Verola Mataveli, Lidiane Raquel, Mariana Fioramonte, Fabio César Gozzo, and Marco Aurélio Zezzi Arruda. "Improving metallomics information related to transgenic and non-transgenic soybean seeds using 2D-HPLC-ICP-MS and ESI-MS/MS." Metallomics 4, no. 4 (2012): 373. http://dx.doi.org/10.1039/c2mt00186a.

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Zittermann, Armin, Jana B. Ernst, Tobias Becker, Jens Dreier, Cornelius Knabbe, Jan F. Gummert, and Joachim Kuhn. "Measurement of Circulating 1,25-Dihydroxyvitamin D: Comparison of an Automated Method with a Liquid Chromatography Tandem Mass Spectrometry Method." International Journal of Analytical Chemistry 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/8501435.

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Background. The clinical relevance of circulating 1,25-dihydroxyvitamin D (1,25(OH)2D) is probably underappreciated, but variations in the measurement of this difficult analyte between different methods limit comparison of results.Methods. In 129 clinical samples, we compared a new automated assay with a commercially available liquid chromatography tandem mass spectrometry (LC-MS/MS) kit.Results. Median (interquartile range) 1,25(OH)2D concentrations with the automated assay and the LC-MS/MS method were 26.6 pg/mL (18.5–39.0 pg/mL) and 23.6 pg/mL (16.1–31.3 pg/mL), respectively (P=0.001). Using the method-specific cut-offs for deficient 1,25(OH)2D levels (<20 pg/mL for the automated assay and <17 pg/mL for the LC-MS/MS method), the percentage of patients classified as 1,25(OH)2D deficient was 28.7% and 27.1%, respectively. However, concordance between the two methods for deficient levels was only 62% and the concordance correlation coefficient was poor (0.534). The regression equation resulted in an intercept of −1.99 (95% CI: −7.33–1.31) and a slope of 1.27 (95% CI: 1.04–1.52) for the automated assay. The mean bias with respect to the mean of the two methods was −3.8 (1.96 SD: −28.3–20.8) pg/mL for the LC-MS/MS method minus the automated assay.Conclusions. The two methods show only modest correlation and further standardization is required to improve reliability and comparability of 1,25(OH)2D test procedures.

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徐, 勇. "Preparation and Characterization of Graphene Induced Quasi 2D Nano Aluminum Oxide." Material Sciences 10, no. 08 (2020): 631–38. http://dx.doi.org/10.12677/ms.2020.108077.

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Cassiano, NM, JC Barreiro, RV Oliveira, and QB Cass. "Direct bioanalytical sample injection with 2D LC–MS." Bioanalysis 4, no. 22 (November 2012): 2737–56. http://dx.doi.org/10.4155/bio.12.226.

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Smolders, J., P. Menheere, A. Kessels, J. Damoiseaux, and R. Hupperts. "Association of vitamin D metabolite levels with relapse rate and disability in multiple sclerosis." Multiple Sclerosis Journal 14, no. 9 (July 24, 2008): 1220–24. http://dx.doi.org/10.1177/1352458508094399.

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Background Multiple Sclerosis is associated with low serum levels of 25-hydroxyvitamin D (25(OH)D). We investigated the association between serum levels of 25(OH)D and 1,25-dihydroxyvitamin D (1,25(OH)2D), the biologically active metabolite, and clinical MS severity as expressed by EDSS-score and relapse rate. Study-design Cross-sectional study. Patients and Methods Serum samples from 267 MS patients were collected for 25(OH)D and 1,25(OH)2D measurement. Clinical MS parameters at the date of serum sampling were determined. Results: Both metabolite levels were significantly lower in the progressive forms compared to the relapsing remitting (RR)MS phenotype. In RRMS patients (disease course ≤ 5 years), high 25(OH)D levels were associated with a high chance of remaining relapse-free. Low 25(OH)D levels were associated with high EDSS-scores. 1,25(OH)2D was not directly associated with relapse rate or EDSS-score, and was dependent of age and 25(OH)D level. Conclusion Serum levels of 25(OH)D were associated with both relapse rate and disability in MS patients. These results are suggestive for a disease modulating effect of the serum concentrations of 25(OH)D on MS. The low circulating 1,25(OH)2D levels in progressive MS are due to older age and lower 25(OH)D levels. The potential consequences for vitamin D supplementation in MS will be discussed.

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Chen, Zhen, Lu Long, Kun Wang, Facai Cui, Lepan Zhu, Ya Tao, Qiong Wu, et al. "Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS." Oncotarget 7, no. 23 (April 27, 2016): 34022–37. http://dx.doi.org/10.18632/oncotarget.9067.

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Anandgaonkar, Vaibhav, Abhishek Gupta, Srinivas Kona, and M. V. N. Kumar Talluri. "Isolation, LC–MS/MS and 2D-NMR characterization of alkaline degradants of tenofovir disoproxil fumarate." Journal of Pharmaceutical and Biomedical Analysis 107 (March 2015): 175–85. http://dx.doi.org/10.1016/j.jpba.2014.12.034.

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Wang, Jian, Jinjin Zhou, Yu Xu, Bingqi Zhu, and Yong Jin. "Characterization of two unknown impurities in roxithromycin by 2D LC–QTOF/MS/MS and NMR." Journal of Pharmaceutical and Biomedical Analysis 184 (May 2020): 113196. http://dx.doi.org/10.1016/j.jpba.2020.113196.

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Feng, Huixing, Xi Li, Dandan Niu, and Wei Ning Chen. "Protein profile in HBx transfected cells: A comparative iTRAQ-coupled 2D LC-MS/MS analysis." Journal of Proteomics 73, no. 8 (June 2010): 1421–32. http://dx.doi.org/10.1016/j.jprot.2009.12.004.

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Stevens, A. L., J. S. Wishnok, S. R. Tannenbaum, and A. J. Grodzinsky. "A28 A 2D-LC/MS/MS APPROACH TO IN VITRO JOINT INJURY BIOMARKERS AND BIOLOGY." Osteoarthritis and Cartilage 14 (2006): S31. http://dx.doi.org/10.1016/s1063-4584(07)60481-6.

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Sun, Xianjun, Yubao Lv, Junjun Wang, HuiQin Cheng, Jianhua Huang, Yijie Du, and Jingcheng Dong. "Differential protein expression profiling by iTRAQ‐2D‐LC‐MS/MS of rats treated with oxaliplatin." Journal of Cellular Biochemistry 120, no. 10 (June 25, 2019): 18128–41. http://dx.doi.org/10.1002/jcb.29116.

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Ye, Feng, Huoming Zhang, Yi-Xuan Yang, Huai-Dong Hu, Siu Kwan Sze, Wei Meng, Jingru Qian, et al. "Comparative proteome analysis of 3T3-L1 adipocyte differentiation using iTRAQ-coupled 2D LC-MS/MS." Journal of Cellular Biochemistry 112, no. 10 (September 19, 2011): 3002–14. http://dx.doi.org/10.1002/jcb.23223.

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Ross, Charles W., William J. Simonsick,, and David J. Aaserud. "Application of Stored Waveform Ion Modulation 2D-FTICR MS/MS to the Analysis of Complex Mixtures." Analytical Chemistry 74, no. 18 (September 2002): 4625–33. http://dx.doi.org/10.1021/ac0201833.

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Liu, Xiaoyan, Zhengguang Guo, Wei Liu, Wei Sun, and Chao Ma. "Differential proteome analysis of hippocampus and temporal cortex using label-free based 2D-LC-MS/MS." Journal of Proteomics 165 (August 2017): 26–34. http://dx.doi.org/10.1016/j.jprot.2017.06.008.

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Golizeh, Makan, and Lekha Sleno. "Optimized proteomic analysis of rat liver microsomes using dual enzyme digestion with 2D-LC–MS/MS." Journal of Proteomics 82 (April 2013): 166–78. http://dx.doi.org/10.1016/j.jprot.2013.02.001.

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Yang, Qi, Sijia Tang, Jie Rang, Mingxing Zuo, Xuezhi Ding, Yunjun Sun, Pinghui Feng, and Liqiu Xia. "Detection of Toxin Proteins from Bacillus thuringiensis Strain 4.0718 by Strategy of 2D-LC–MS/MS." Current Microbiology 70, no. 4 (December 5, 2014): 457–63. http://dx.doi.org/10.1007/s00284-014-0747-9.

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Vamathevan, Veronica, and Edwin J. Murby. "Accurate analysis of testosterone in human serum using a heart-cutting 2D-UPLC–MS/MS procedure." Journal of Chromatography B 1038 (December 2016): 49–56. http://dx.doi.org/10.1016/j.jchromb.2016.10.004.

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Wasnik, Samiksha, Isha Sharma, David J. Baylink, and Xiaolei Tang. "Vitamin D as a Potential Therapy for Multiple Sclerosis: Where Are We?" International Journal of Molecular Sciences 21, no. 9 (April 28, 2020): 3102. http://dx.doi.org/10.3390/ijms21093102.

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Multiple sclerosis (MS) is a chronic demyelinating disease of the central nervous system and is caused by an aberrant immune response to myelin sheath. Disease-modifying medications, which mainly aim to suppress such aberrant immune response, have significantly improved MS treatment. However, the disease severity continues to worsen. In contrast, progressively more data suggest that 1,25-dihydroxyvitamin D or 1,25(OH)2D, i.e., the active vitamin D, suppresses the differentiation of potentially pathogenic T cells associated with MS, enhances the differentiation of regulatory T cells that suppress the pathogenic T cells, and promotes remyelination. These novel 1,25(OH)2D functions have encouraged investigators to develop vitamin D as a potential therapy for MS. However, because of the hypercalcemia that is associated with high 1,25(OH)2D concentrations, supplementation of native vitamin D has been a major focus in clinical trials for the treatment of MS, but such trials have produced mixed data. In this article, we will review current progress in the supplementation of different vitamin D forms for the treatment of experimental autoimmune encephalomyelitis (i.e., an MS animal model) as well as MS. Furthermore, we will review alternative strategies that our laboratory and others are pursuing in an attempt to circumvent the hurdles that are hampering the effective use of vitamin D as a potential therapy for MS.

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Wang, Yong, Yi Ren Jiang, and Li Qin. "Using 2D-PAGE and Mass Spectrometry (LC-MS/MS) for Embryo Proteins Analysis in Antheraea pernyi." Advanced Materials Research 884-885 (January 2014): 556–59. http://dx.doi.org/10.4028/www.scientific.net/amr.884-885.556.

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To study the dynamic variation of protein components and contents inAntheraea pernyi, 2D-PAGE, in this job, was used to investigate embryo development. Some special protein spots expression profiles changed obviously were analyzed by mass spectrometry. 2D-PAGE results showed that the protein spots increased from 370 at the development stage of 12 h to 422 at 300 h. Eighteen special protein spots were identified byLC-MS/MS, and 8 kinds of proteins are found to include heat shock proteins families, Vitellogenin, KK-42-binding protein, actin-depolymerizing factor 1, etc., which are mainly involved in some important processes. Analyze the function of those proteins, so as to accumulate basic information on embryonic development inA. pernyi.

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Soumia, Kharfouchi, and Houria Djafri. "On unilateral 2D Markov-Switching Autoregressive (MS-AR) model." International Journal of Mathematics in Operational Research 1, no. 1 (2020): 1. http://dx.doi.org/10.1504/ijmor.2020.10033953.

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Tastet, Laure, Dirk Schaumlöffel, Brice Bouyssiere, and Ryszard Lobinski. "Identification of selenium-containing proteins in selenium-rich yeast aqueous extract by 2D gel electrophoresis, nanoHPLC–ICP MS and nanoHPLC–ESI MS/MS." Talanta 75, no. 4 (May 30, 2008): 1140–45. http://dx.doi.org/10.1016/j.talanta.2008.01.003.

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Szalwinski, Lucas J., L. Edwin Gonzalez, Nicolás M. Morato, Brett M. Marsh, and R. Graham Cooks. "Bacterial growth monitored by two-dimensional tandem mass spectrometry." Analyst 147, no. 5 (2022): 940–46. http://dx.doi.org/10.1039/d1an01901e.

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Zemánková, Kristýna, Kristýna Pavelicová, Antonio Pompeiano, Ludmila Mravcová, Martin Černý, Kamila Bendíčková, Marcela Hortová Kohoutková, et al. "Targeted volatolomics of human monocytes: Comparison of 2D‐GC/TOF‐MS and 1D‐GC/Orbitrap‐MS methods." Journal of Chromatography B 1184 (November 2021): 122975. http://dx.doi.org/10.1016/j.jchromb.2021.122975.

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Zemánková, Kristýna, Kristýna Pavelicová, Antonio Pompeiano, Ludmila Mravcová, Martin Černý, Kamila Bendíčková, Marcela Hortová Kohoutková, et al. "Targeted volatolomics of human monocytes: Comparison of 2D‐GC/TOF‐MS and 1D‐GC/Orbitrap‐MS methods." Journal of Chromatography B 1184 (November 2021): 122975. http://dx.doi.org/10.1016/j.jchromb.2021.122975.

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Wei, Chun-Hui, Ming-Yu Lai, and Xi-Jing Mo. "Screening differentially expressed serum proteins in gastric cancer by iTRAQ labeling coupled with 2D-LC-MS/MS." World Chinese Journal of Digestology 21, no. 36 (2013): 4146. http://dx.doi.org/10.11569/wcjd.v21.i36.4146.

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Verleyen, Peter, Geert Baggerman, Wannes D’Hertog, Evy Vierstraete, Steven J. Husson, and Liliane Schoofs. "Identification of new immune induced molecules in the haemolymph of Drosophila melanogaster by 2D-nanoLC MS/MS." Journal of Insect Physiology 52, no. 4 (April 2006): 379–88. http://dx.doi.org/10.1016/j.jinsphys.2005.12.007.

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Watson, Gregory W., Samanthi Wickramasekara, Claudia S. Maier, David E. Williams, Roderick H. Dashwood, and Emily Ho. "Assessment of global proteome in LNCaP cells by 2D-RP/RP LC–MS/MS following sulforaphane exposure." EuPA Open Proteomics 9 (December 2015): 34–40. http://dx.doi.org/10.1016/j.euprot.2015.08.002.

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Xia, Hailing, Lingfang Feng, Lijun Lin, Zhaoqiang Jiang, Junqiang Chen, Wei Shi, Shibo Ying, et al. "Exploration of identifying novel serum biomarkers for malignant mesothelioma using iTRAQ combined with 2D-LC-MS/MS." Environmental Research 193 (February 2021): 110467. http://dx.doi.org/10.1016/j.envres.2020.110467.

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Ajitomi, Satori, Juichi Fujimori, and Ichiro Nakashima. "Usefulness of two-dimensional measurements for the evaluation of brain volume and disability in multiple sclerosis." Multiple Sclerosis Journal - Experimental, Translational and Clinical 8, no. 1 (January 2022): 205521732110707. http://dx.doi.org/10.1177/20552173211070749.

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Background Two-dimensional (2D) measures have been proposed as potential proxies for whole-brain volume in multiple sclerosis (MS). Objective To verify whether 2D measurements by routine MRI are useful in predicting brain volume or disability in MS. Methods In this cross-sectional analysis, eighty-five consecutive Japanese MS patients—relapsing-remitting MS (81%) and progressive MS (19%)—underwent 1.5 Tesla T1-weighted 3D MRI examinations to measure whole-brain and grey matter volume. 2D measurements, namely, third ventricle width, lateral ventricle width (LVW), brain width, bicaudate ratio, and corpus callosum index (CCI), were obtained from each scan. Correlations between 2D measurements and 3D measurements, the Expanded Disability Status Scale (EDSS), or processing speed were analysed. Results The third and lateral ventricle widths were well-correlated with the whole-brain volume ( p < 0.0001), grey matter volume ( p < 0.0001), and EDSS scores ( p = 0.0001, p = .0004, respectively).The least squares regression model revealed that 78% of the variation in whole-brain volume could be explained using five explanatory variables, namely, LVW, CCI, age, sex, and disease duration. By contrast, the partial correlation coefficient excluding the effect of age showed that the CCI was significantly correlated with the EDSS and processing speed ( p < 0.0001). Conclusion Ventricle width correlated well with brain volumes, while the CCI correlated well with age-independent (i.e. disease-induced) disability.

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Zheng, Fei-Xia, Sheng Li, Chuan Ding, Jian-Tao Zhao, and Jian Ruan. "Theoretical and experimental research on the cartridge two-dimensional (2D) electro-hydraulic servo valve." Advances in Mechanical Engineering 13, no. 2 (February 2021): 168781402199653. http://dx.doi.org/10.1177/1687814021996532.

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A cartridge 2D EHSV based on torque motor is proposed in this paper, which is keeping the advantages of conventional 2D valve, such as compact structure, high power-to-weight ratio, and excellent dynamic characteristics. In order to explore its dynamic characteristics, the design concept and operating principle of the cartridge 2D EHSV valve is presented first. Then the mathematical model of cartridge 2D EHSV valve is derived, especially the rotational viscous damping coefficient which is studied in detail. The simulation results of the open(closed)-loop model show that the support pressure plays an insignificant role in the dynamic characteristics of 2D EHSV, only the phase bandwidth of the closed-loop model increases. And the rise time of the open-loop model is about 10 ms, and the frequency bandwidth is about 40 Hz, while the rise time is about 4 ms and the frequency bandwidth is about 100 Hz of the closed-loop model. Furthermore, as verified by experimental results, the rising time of the step response is about 7 ms and the bandwidth is approximately 38 Hz under the open-loop control, while that is 6 ms and 117 Hz for 25% input signal under the close-loop control. Finally, the difference between the experiment and the simulation is discussed. It is concluded that the cartridge 2D EHSV has excellent dynamic characteristics.

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Chen, Hongyan, Mengke Wang, and Weichun Huang. "Two-Dimensional Selenium Nanosheet-Based Sponges with Superior Hydrophobicity and Excellent Photothermal Performance." Nanomaterials 12, no. 21 (October 26, 2022): 3756. http://dx.doi.org/10.3390/nano12213756.

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Photothermally assisted superhydrophobic materials play an important role in a variety of applications, such as oil purification, waste oil collection, and solar desalination, due to their facile fabrication, low-cost, flexibility, and tunable thermal conversion. However, the current widely used superhydrophobic sponges with photothermal properties are usually impaired by a high loading content of photothermal agents (e.g., gold or silver nanoparticles, carbon nanotubes), low photothermal efficiency, and require harmful processes for modification. Here, a one-pot, simple composite consisting of two-dimensional (2D) selenium (Se) nanosheets (NSs) and commercially used melamine sponge (MS) is rationally designed and successfully fabricated by a facile dip-coating method via physical adsorption between 2D Se NSs and MS. The loading content of 2D Se NSs on the skeleton of the MS can be well controlled by dipping cycle. The results demonstrate that after the modification of 2D Se NSs on the MS, the wettability transition from hydrophilicity to hydrophobicity can be easily achieved, even at a very low loading of 2D Se NSs, and the highly stable photothermal conversion of the as-fabricated composites can be realized with a maximum temperature of 111 ± 3.2 °C due to the excellent photothermal effect of 2D Se NSs. It is anticipated that this composite will afford new design strategies for multifunctional porous structures for versatile applications, such as high-performance solar desalination and photothermal sterilization.

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Wang, Ting Yu, Hua Zhou, Yuen Fan Wong, Pui Kei Wu, Wen-Luan Wendy Hsiao, Elaine Lai-Han Leung, and Liang Liu. "The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–15. http://dx.doi.org/10.1155/2013/582493.

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Qingfu Guanjieshu (QFGJS) is an herbal preparation for treating rheumatoid arthritis (RA). Previous studies revealed that QFGJS significantly inhibited experimental arthritis and acute inflammation, accompanied by reduction of proinflammatory cytokines and elevation of anti-inflammatory cytokines. This study aims to identify the targeted proteins and predict the proteomic network associated with the drug action of QFGJS by using 2D gel and MALDI-TOF-MS/MS techniques. Thirty female Wistar rats were evenly grouped as normal and vehicle- and QFGJS-treated CIA rats. The antiarthritic effect of QFGJS was examined with a 19-day treatment course, and the knee synovial tissues of animals from each group were obtained for 2D gel and MALDI-TOF-MS/MS analysis. Results showed that QFGJS significantly ameliorated collagen II-induced arthritis when administrated at 2.8 g/kg body weight for 19 days. 2D gel image analysis revealed 89 differentially expressed proteins in the synovial tissues among the normal and vehicle- and QFGJS-treated CIA rats from over 1000 proteins of which 63 proteins were identified by MALDI-TOF-MS/MS analysis, and 32 proteins were included for classification of functions using Gene Ontology (GO) method. Finally, 14 proteins were analyzed using bioinformatics, and a predicted proteomic network related to the anti-arthritic effect of QFGJS was established, and Pgk1 plays a central role.

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Berendt, F., T. Froehlich, M. Boelhauve, T. Guengoer, E. Wolf, and G. J. Arnold. "239 OOCYTE MATURATION IN VITRO: PROTEOME ALTERATIONS ANALYZED BY ULTRA-SENSITIVE FLUOROCHROME LABELING AND NANO-ESI-MS-MS." Reproduction, Fertility and Development 20, no. 1 (2008): 199. http://dx.doi.org/10.1071/rdv20n1ab239.

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In mammals, oocytes are arrested in the diplotene stage of the first prophase until the beginning of the estrous cycle. Directly before ovulation, the oocyte completes the first meiotic division and progresses to metaphase II. This process is mimicked by oocyte maturation in vitro, which is critical in assisted reproduction techniques in humans and animals. A sensitive 2-dimensional (2D) difference gel electrophoresis (DIGE) saturation labeling approach including an internal pooled standard was used for quantitative proteome profiling of immature v. in vitro-matured bovine oocytes. A mixture of 0.25 µg of oocyte sample and 0.25 µg of the internal pooled standard was separated on each analytical 2D gel, corresponding to the protein content of approximately five oocytes. In total, the study comprised 48 2D gel images representing 24 DIGE experiments [6 independent samples � 2 gels per stage (immature/mature) � 2 pH gradients]. DeCyder differential in-gel analysis (DIA; Amersham Biosciences, Piscataway, NJ, USA) detected 2244 spots (SD = 100) in pH 4–7 images, and 1291 spots (SD = 65) in pH 6–9 images. In total, 38 spots with different intensity were detected (abundance ratio ≥2; Student's t-test, P ≤ 0.01). Differently abundant spots were identified by nano-LC-MS/MS analysis of matched spots in a preparative gel of saturation-labeled protein extract from 2200 immature oocytes. Ten spots could be unambiguously identified and comprised, e.g., interesting proteins such as clusterin, 14-3-3 ε, redox enzymes, and new polymorphic forms of glutathione-S-transferases. Clusterin is suggested to interact with components of the complement membrane attack complex, and 14-3-3 ε is a mediator of the activation of the maturation promoting factor, a key enzyme of the meiosis. Our study demonstrates the feasibility of differential oocyte proteomics using saturation labeling. Several of the proteins identified in this study are already known to play an important role in oocyte maturation, which reflects the relevance and reliability of our approach. Furthermore, we found proteins which are so far unknown in the context of oocyte development. A more detailed knowledge of the role of these candidates during oocyte maturation may help to optimize the in vitro-maturation process in order to increase the rate of successful in vitro fertilization and other assisted reproduction techniques in cattle and other mammals. This work was supported by grant Deutsche Forschungsgemeinschaft (FOR 478, GRK 1029).

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Laha, Thomas J., Frederick G. Strathmann, Zhican Wang, Ian H. de Boer, Kenneth E. Thummel, and Andrew N. Hoofnagle. "Characterizing Antibody Cross-reactivity for Immunoaffinity Purification of Analytes prior to Multiplexed Liquid Chromatography–Tandem Mass Spectrometry." Clinical Chemistry 58, no. 12 (December 1, 2012): 1711–16. http://dx.doi.org/10.1373/clinchem.2012.185827.

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BACKGROUND Immunoassays for 1α,25-dihydroxyvitamin D [1α,25(OH)2D] lack analytical specificity. We characterized the cross-reactivity of an anti-1α,25(OH)2D antibody with purified vitamin D metabolites and used these data to map the chemical features of 1α,25(OH)2D that are important for antibody binding. Additionally, we hypothesized that when combined with isotope-dilution liquid chromatography–tandem mass spectrometry (LC-MS/MS), antibody cross-reactivity could be used to semiselectively enrich for structurally similar metabolites of vitamin D in a multiplexed assay. METHODS Sample preparation consisted of immunoaffinity enrichment with a solid-phase anti-1α,25(OH)2D antibody and derivatization. Analytes were quantified with LC-MS/MS. Supplementation and recovery studies were performed for 11 vitamin D metabolites. We developed a method for simultaneously quantifying 25(OH)D2, 25(OH)D3, 24,25(OH)2D3, 1α,25(OH)2D2, and 1α,25(OH)2D3 that included deuterated internal standards for each analyte. RESULTS The important chemical features of vitamin D metabolites for binding to the antibody were (a) native orientation of the hydroxyl group on carbon C3 in the A ring, (b) the lack of substitution at carbon C4 in the A ring, and (c) the overall polarity of the vitamin D metabolite. The multiplexed method had lower limits of quantification (20% CV) of 0.2 ng/mL, 1.0 ng/mL, 0.06 ng/mL, 3.4 pg/mL, and 2.8 pg/mL for 25(OH)D2, 25(OH)D3, 24,25(OH)2D3, 1α,25(OH)2D2, and 1α,25(OH)2D3, respectively. Method comparisons to 3 other LC-MS/MS methods yielded an r2 value >0.9, an intercept less than the lower limit of quantification, and a slope statistically indistinguishable from 1.0. CONCLUSIONS LC-MS/MS can be used to characterize antibody cross-reactivity, a conclusion supported by our multiplexed assay for 5 vitamin D metabolites with immunoenrichment in a targeted metabolomic assay.

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Journal articles on the topic '2D MS/MS'

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