Relevant bibliographies by topics / 2D MS/MS

Academic literature on the topic '2D MS/MS'

Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "2D MS/MS"

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Delahunty, Claire, and John R. Yates III. "Protein identification using 2D-LC-MS/MS." Methods 35, no. 3 (March 2005): 248–55. http://dx.doi.org/10.1016/j.ymeth.2004.08.016.

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Ketha, Hemamalini, Rajiv Kumar, and Ravinder J. Singh. "LC-MS/MS for Identifying Patients with CYP24A1 Mutations." Clinical Chemistry 62, no. 1 (January 1, 2016): 236–42. http://dx.doi.org/10.1373/clinchem.2015.244459.

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Abstract BACKGROUND Patients have been described with loss-of-function CYP24A1 (cytochrome P450, family 24, subfamily A, polypeptide 1) mutations that cause a high ratio of 25-hydroxyvitamin D to 24,25-dihydroxyvitamin D [25(OH)D/24,25(OH)2D], increased serum 1,25-dihydroxyvitamin D, and resulting hypercalcemia, hypercalciuria and nephrolithiasis. A 25(OH)D/24,25(OH)2D ratio that can identify patients who are candidates for confirmatory CYP24A1 genetic testing would be valuable. We validated an LC-MS/MS assay for 24,25(OH)2D (D3 and D2) and determined a 25(OH)D/24,25(OH)2D cutoff to identify candidates for confirmatory genetic testing. METHODS After addition of isotope-labeled internal standard, serum samples were extracted by solid-phase extraction, derivatized with 4-phenyl-1,2,4,-triazoline-3,5-dione, and quantified by LC-MS/MS. We measured 25(OH)D/24,25(OH)2D in 91 healthy patients and 34 patients with clinically suspected CYP24A1-mediated hypercalcemia. RESULTS The limits of detection and quantification were 0.03 (0.2) and 0.1 (0.24) nmol/L, respectively, for 24,25(OH)2D3, and 0.1 (0.23) and 0.5 (1.16) nmol/L for 24,25(OH)2D2. Intra- and interassay imprecision was 4%–15% across the analytical measurement range of 0.1–25 ng/mL (0.2–60 nmol/L). No interference was observed with 25(OH)D and 1,25(OH)2D. 25(OH)D/24,25(OH)2D of 7–35 was observed in healthy patients, whereas in 2 patients with CYP24A1 mutations, 25(OH)D/24,25(OH)2D was significantly increased (99–467; P < 0.001). A 25(OH)D/24,25(OH)2D ratio ≥99 identified patients who were candidates for CYP24A1 genetic testing. CONCLUSIONS Increased 25(OH)D/24,25(OH)2D supports the diagnosis of reduced CYP24A1 activity due to mutations in CYP24A1. Measurement of 25(OH)D/24,25(OH)2D should be considered a part of the clinical workup in patients with hypercalcemia of otherwise unknown etiology.
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Escoffier, Patricia, Luc Paris, Bahram Bodaghi, Martin Danis, Dominique Mazier, and Carine Marinach-Patrice. "Pooling Aqueous Humor Samples: Bias in 2D-LC-MS/MS Strategy?" Journal of Proteome Research 9, no. 2 (February 5, 2010): 789–97. http://dx.doi.org/10.1021/pr9006602.

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Arrigoni, Giorgio, Celine Fernandez, Cecilia Holm, Michaela Scigelova, and Peter James. "Comparison of MS/MS Methods for Protein Identification from 2D-PAGE." Journal of Proteome Research 5, no. 9 (September 2006): 2294–300. http://dx.doi.org/10.1021/pr0601281.

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Zhang, Lina, Chih-Wei Liu, and Qibin Zhang. "Online 2D-LC-MS/MS Platform for Analysis of Glycated Proteome." Analytical Chemistry 90, no. 2 (December 27, 2017): 1081–86. http://dx.doi.org/10.1021/acs.analchem.7b03342.

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Xiao, Mingming, Yajing Chen, Huan Yu, Su Wei, Kang Yu, Huan Zhao, and Ruibing Chen. "Analysis of the whole serum proteome using an integrated 2D LC-MS/MS system." Anal. Methods 6, no. 18 (2014): 7157–60. http://dx.doi.org/10.1039/c4ay01274g.

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Xu, Jiyu, Shuxin Zheng, Mimi Li, Xiaoyan Liu, Haidan Sun, Zhengguang Guo, Jing Wei, Lulu Jia, and Wei Sun. "A Comprehensive 2D-LC/MS/MS Profile of the Normal Human Urinary Metabolome." Diagnostics 12, no. 9 (September 9, 2022): 2184. http://dx.doi.org/10.3390/diagnostics12092184.

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Profiling bodily fluids is crucial for monitoring and discovering metabolic markers of disease. In this study, a comprehensive analysis approach based on 1D-LC-MS/MS and 2D-LC-MS/MS was applied to profile normal human urine metabolites from 348 children and 315 adults. A total of 2357 metabolites were identified, including 1831 endogenous metabolites and 526 exogenous ones. In total, 1005 metabolites were identified in urine for the first time. The urinary metabolites were mainly involved in amino acid metabolism, small molecule biochemistry, lipid metabolism and cellular compromise. The comparison of adult’s and children’s urine metabolomes showed adults urine had more metabolites involved in immune response than children’s, but the function of binding of melatonin, which belongs to the endocrine system, showed a higher expression in children. The urine metabolites detected by the 1D-LC-MS/MS method were mainly related to amino acid metabolism and lipid metabolism, and the 2D-LC-MS/MS method not only explored metabolites from 1D-LC-MS/MS but also metabolites related to cell signaling, cell function and maintenance, etc. Our analysis comprehensively profiled and functionally annotated the metabolome of normal human urine, which would benefit the application of urinary metabolome to clinical research.
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Rao, R. Nageswara, R. Mastan Vali, and Dhananjay D. Shinde. "On-line 2D-LC-ESI/MS/MS determination of rifaximin in rat serum." Biomedical Chromatography 23, no. 11 (November 2009): 1145–50. http://dx.doi.org/10.1002/bmc.1236.

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Lenglois, S., M. Moser, and A. O. A. Miller. "Microsupport with Two-Dimensional Geometry (2D-MS)." Cytotechnology 44, no. 1/2 (2004): 47–54. http://dx.doi.org/10.1023/b:cyto.0000043403.20008.02.

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Bekkach, Yasin, Annemieke C. Heijboer, Erik Endert, and Mariëtte T. Ackermans. "Determination of urinary aldosterone using a plasma aldosterone 2D ID LC–MS/MS method." Bioanalysis 8, no. 17 (September 2016): 1765–75. http://dx.doi.org/10.4155/bio-2016-0115.

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Dissertations / Theses on the topic "2D MS/MS"

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Joyner, Jeffrey Clark. "The Use of 2D-LC-MS/MS in disease characterization and global proteomics." Connect to resource, 2006. http://hdl.handle.net/1811/6604.

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Thesis (Honors)--Ohio State University, 2006.
Title from first page of PDF file. Document formatted into pages: contains 46 p.; also includes graphics. Includes bibliographical references (p. 46). Available online via Ohio State University's Knowledge Bank.
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Vieira, José Cavalcante Souza. "Investigação de metalotioneínas em peixes da região de Jirau - bacia do Rio Madeira - Rondônia." Botucatu, 2017. http://hdl.handle.net/11449/150736.

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Orientador: Pedro de Magalhães Padilha
Resumo: Devido a sua grande concentração de nutrientes, tais como proteínas, vitaminas e minerais, o peixe é considerado um dos alimentos mais saudáveis que se pode encontrar na natureza. No entanto, a ingestão de peixes é considerada a forma predominante de via de exposição do ser humano ao mercúrio (Hg), principalmente para as populações que vivem às margens dos rios, onde o peixe constitui a principal fonte de proteína. Na tentativa de elucidar os mecanismos de toxicidade das espécies mercuriais, o teor desse metal tem sido estudado intensamente pela comunidade científica nas últimas décadas em amostras de solo, sedimentos, humanos e peixes na Amazônia brasileira. Sabe-se que as espécies mercuriais bioacumuladas nos tecidos dos seres vivos ligam-se a metaloproteínas, e quando há uma concentração alta de metal tóxico nos organismos, esses passam a expressar proteínas de defesa, denominadas metalotioneínas (MTs) responsáveis pelo transporte e eliminação de metais tóxicos. Apesar de estudos mostrarem o aumento das metalotioneínas em animais expostos a metais potencialmente tóxicos, essas proteínas não foram caracterizadas para confirmação de sua veridicidade, são analisadas por métodos indiretos, esse fato leva a necessidade de técnicas mais precisas na identificação de metalotioneínas. Levando em consideração o exposto esse estudo teve como objetivo otimizar métodos de quantificação de mercúrio e técnica de eletroforese para identificação de possíveis metalotioneínas biomarcadoras d... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Due to its high concentration of nutrients, such as proteins, vitamins and minerals, fish is considered one of the healthiest foods that one can find in nature. However, fish intake is considered to be the predominant human exposure pathway to mercury (Hg), especially for populations living along riverbanks where fish are the main source of protein. In the attempt to elucidate the toxicity mechanisms of mercurial species, the content of this metal has been intensively studied by the scientific community in recent decades in soil, sediment, human and fish samples in the Brazilian Amazon. It is known that mercurial species bioaccumulated in the tissues of living beings bind to metalloproteins, and when there is a high concentration of toxic metal in organisms, they begin to express defense proteins, called metallothioneins (MTs) responsible for the transport and elimination of Toxic metals. Although studies have shown the increase of metallothioneins in animals exposed to potentially toxic metals, these proteins have not been characterized to confirm their veridicity, are analyzed by indirect methods, this fact leads to the need for more precise techniques in the identification of metallothioneins. Taking into account the above, this study aimed to optimize mercury quantification methods and electrophoresis technique for identification of possible mercury biomarkers metallothionein in muscular and hepatic tissue of fish of economic interest, Tucunaré (Cichla spp.), Filhote (Bra... (Complete abstract click electronic access below)
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Bittarello, Alis Correia. "Estudo de biomarcadores de mercúrio em peixes da amazônia por meio da metalômica e análise do estresse oxidativo." Botucatu, 2017. http://hdl.handle.net/11449/151174.

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Orientador: Pedro de Magalhães Padilha
Resumo: O mercúrio é um metal tóxico, de distribuição ubíqua, com capacidade para bioacumulação e biomagnificação, que provoca alterações em biomoléculas importantes no metabolismo e contribui para o estabelecimento do estresse oxidativo em organismos aquáticos. Logo, o presente estudo teve por objetivo identificar e avaliar possíveis biomarcadores proteicos e/ou enzimáticos da toxicidade do mercúrio em peixes da região amazônica, por meio do estudo metaloproteômico e avaliação do estresse oxidativo. Foram utilizadas metodologias de fracionamento e identificação de proteínas por eletroforese bidimensional (2D PAGE) associada à espectrometria de massas (MS), mapeamento do mercúrio, em spots proteicos, por espectrometria de absorção atômica em forno de grafite (GFAAS) e avaliação de marcadores de estresse oxidativo. As espécies utilizadas foram o Plagioscion squamosissimus (corvina) e Colossoma macropomum (tambaqui), coletados na área da Usina Hidrelétrica de Jirau (rio Madeira-RO), que foram selecionadas em função da abundância populacional, interesse para a pesca e posição diferente na cadeia trófica (carnívoro e onívoro, respectivamente). Os tecidos amostrados foram o hepático, renal e muscular. Os resultados obtidos demonstraram maior concentração de mercúrio total no P. squamosissimus, espécie carnívora, e padrão de distribuição deste elemento igual para ambas as espécies (fígado>rim>músculo). Há tendência para maior atividade enzimática nos tecidos hepático e renal da espécie com... (Resumo completo, clicar acesso eletrônico abaixo)
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Nakata, Michael Takeshi. "Simulating the FTICR-MS Signal of a Decaying Beryllium-7 Ion Plasma in a 2D Electrostatic PIC Code." Diss., CLICK HERE for online access, 2010. http://contentdm.lib.byu.edu/ETD/image/etd3370.pdf.

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Eckberg, Melanie N. "Forensic Toxicological Screening and Confirmation of 800+ Novel Psychoactive Substances by LC-QTOF-MS and 2D-LC Analysis." FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3923.

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Novel psychoactive substances (NPS) represent a great challenge to toxicologists due to the ability of illicit drug manufacturers to alter NPS chemical structures quickly and with ease to circumvent legislation regulating their use. Each time a new structure is introduced, there is a possibility that it has not been previously recorded in law enforcement or scientific databases. Many toxicology laboratories use targeted analytical methods that rely on libraries of known compounds to identify drugs in samples. However, these libraries do not include large numbers of NPS which could result in non-identification or detection. High-resolution mass spectrometry (HRMS) has been suggested as a method for screening a wide variety of analytes due to its higher sensitivity and mass accuracy as compared to some other forms of mass spectrometry. This technique can generate characteristic MS/MS spectral data for use in compound identification. The main goal of this research was to create a high-resolution mass spectrometry (HRMS) library of NPS and metabolites, as well as validate a method for screening and confirmation of these substances. The study consisted of three main tasks which included; the development of a large high-resolution MS/MS spectral library and database, validation of a method for screening and confirmation of over 800 NPS and metabolites, and screening of blind-spiked and authentic urine specimens to determine real-world applicability of the HRMS library and method. During validation, several isomeric and structurally related NPS were observed which could not be adequately separated using traditional LC methods. A fourth task was therefore added to investigate improved separation using two-dimensional liquid chromatography (2D-LC). Increased resolving power is achieved in 2D-LC through the coupling of multiple orthogonal separation systems. Ultimately, an on-line, comprehensive method was developed using orthogonal reversed-phase columns in each dimension (RP x RP) for improved separation of co-eluting and isomeric synthetic cannabinoids. This work can aid laboratories in the identification of NPS through the use of a validated LC-QTOF-MS method for screening and confirmation and HRMS spectral library. In instances where isomeric and structurally related NPS are not sufficiently separated, RP x RP methods can be explored.
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Howard, James W. "The development of mass spectrometry-based methodologies for the high throughput quantitation of peptides in biological matrices." Thesis, Loughborough University, 2018. https://dspace.lboro.ac.uk/2134/32454.

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The aim of this research was the development of mass spectrometry-based methodologies for the high-throughput quantitation of peptides in biological matrices. Glucagon and GLP-1, which are of interest as biomarkers and in the development of therapeutics, were chosen as model peptides. Immunoassays that are traditionally used to quantify these often perform poorly; therefore, necessitating the development of alternative methodologies. Application of mass spectrometry-based methodologies to these analytes has, however, been limited, primarily due to sensitivity challenges, but also due to analytical challenges associated with their endogenous nature and instability in biological matrices. Chapter 2 describes the development and qualification of the first liquid-chromatography coupled tandem mass spectrometry (LC-MS/MS) method for the quantitation of endogenous glucagon from human plasma. A novel 2D extraction procedure was developed to ensure robustness and sensitivity, whilst a novel surrogate matrix quantitation strategy took into account the endogenous nature of the analyte. A lower limit of quantitation (LLOQ) of 25 pg/mL was qualified, which was a considerable improvement over that previously reported in the literature (250 pg/mL) for a LC-MS/MS method. Clinical samples were cross-validated against a conventional radioimmunoassay (RIA), and similar pharmacokinetic (PK) profiles resulted, demonstrating that the methods were complementary. In Chapter 2 glucagon instability in biological matrix was noted. To characterise this further, in Chapter 3 in vitro glucagon metabolites were identified using high-resolution mass spectrometry (HRMS). Metabolites observed by others (glucagon19-29, glucagon3 29 and [pGlu]3glucagon3 29) in alternative matrices were identified, alongside novel metabolites (glucagon20-29 and glucagon21-29). Cross-interference of these metabolites in immunoassays may help to explain their poor performance, whilst knowledge of metabolism may also aid the development of future stabilisation strategies. The method developed in Chapter 2 was refined in Chapter 4 to improve sensitivity, robustness and throughput, and to add GLP-1 as a secondary analyte. The sensitivity achieved (glucagon: 15 pg/mL LLOQ, GLP-1: 25 pg/mL LLOQ) is the highest reported for both peptides for an extraction avoiding immunoenrichment. Specificity of endogenous glucagon quantitation was assured using a novel approach with a supercharging mobile phase additive to access a sensitive qualifier transition. A cross-validation against established immunoassays using physiological study samples demonstrated some similarities between the methods. Differences between the immunoassay results exemplified the need to develop alternative methodologies. The resulting LC-MS/MS method is considered a viable alternative to immunoassays, for the quantitation of endogenous glucagon, dosed glucagon and/or dosed GLP-1 in human plasma.
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Niranjane, Ajay Pundaiikrao, and ajay niranjane@gmail com. "Screening diverse cellulase enzymes from the white rot fungus Phlebia gigantea for high activity and large scale applications." RMIT University. Applied Sciences, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080513.150257.

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Cellulosic biomass is the major organic matter produced in the biosphere. The biodegradation of this cellulosic material is achieved by enzymatic activities of the cellulose degrading microorganisms. These organisms usually express a complex extracellular or a membrane bound cellulolytic system comprising combination of several cellulase enzymes. Cellulases are the group of hydrolytic enzymes capable of hydrolysing insoluble cellulose to glucose. Phlebia gigantea is an aggressive white rot basidiomycete with ability to tolerate resinous extracts on freshly cut wood and higher growth rate. This helps the fungus to colonise the sapwood preventing other fungi from becoming established. Early research on the cellulase system of this organism reported the presence of a cellulase system composed of P-glucosidase, endoglucanase and a cellobiohydrolase. Based on these unpublished studies, our aim was to obtain a complete sequence of putative cellobiohydrolase I (CbhI) from this organism. Attempts to identify and isolate the cellulase gene resulted in an incomplete cDNA sequence of I 154 bp. To understand the cellulase system, expression and regulation of the cellulase enzymatic activity was examined for incubation of P. gigantea on substrates glucose, xylose, Avicel, carboxymethyl cellulose and cellobiose. The pH, total protein and biomass production results indicated that the capacity of P. gigantea to degrade cellulose is dependent upon the nature of the carbon source and the regulation of the cellulase synthesis is repressed in the presence of simple sugars like glucose and xylose. The study employed the highly effective method of purification by affinity adsorption and purified cellulase complex in large quantity. Characterisation of the kinetic properties of this cellulase complex revealed that the rate of cellulase catalysis were optimum at pH 5.0 and temperature 50GC. The purified complex was comprised of multiple proteins and demonstrated significant CMCase and CBHase activity on zymogram analysis. The purified cellulase complex was characterised by 2D gel electrophoresis and by peptide mass finger printing using MALDI-TOF massspectrometry analysis. The 2D gel analysis of the purified cellulase complex showed 15 spots within the range of pI 3.5 to pI 7 and the molecular weight between 20KDa to 100KDa. Three protein spots were selected based on the IEF and SDS zymogram and identified using MALDI-TOF MS analysis. These proteins were identified based on the peptide mass data belonging to the 6-phospho-a-glucosidase, p-glucosidase and glycosyl hydrolase family 13 a-amylase or pullulanases, suggesting the divergent evolution of specific cellulase proteins. This study showed P. gigantea as a potential cellulase source and the cellulase complex secreted by the induction of substrate, comprises a variety of enzymes related to hydrolysis of cellulose biomass. It is evident from this and previous studies that P. gigantea cellulase complex comprises of a specific set of enzymes that possess the ability to degrade crystalline cellulose and is one of the first organisms to colonise freshly cut wood. Further studies on the cellulase system of this primary colonist may open up the prospects to utilise this organism as the potential onsite bioreactor agent, pre-treating the biomass and increasing the economic feasibility of the industrial bioenergy processes.
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Halienová, Andrea. "Změny proteomu a metabolomu u vybraných organismů ve stresových podmínkách." Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-233313.

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Living conditions of every organism are influenced by various factors at this time. Some of them have positive effect on organism, some negative. Basic condition for surviving is the ability to resist and adapt to changing metabolic and living conditions. Every single stress effect can lead to changes in metabolism but organisms have ability to develope sufficient mechanisms for stress response. Some of them are similar for all living organisms (enzyme production, endogenous primary stress metabolites) some of them are specific for certain organism or stress type. Cell stress response can be observed on different levels (proteomic, genomic, metabolomic). In proper conditions it can be used indrustrially. In this work, influences of various stress factors were studied. These factors were applied on selected organisms – carotenogenic yeast and plant materials. Yeast stress response was induced by osmotic and oxidation stress factors. Changes on proteomic level and in production of selected secondary metabolites were observed. Proteome was analyzed by 1D and 2D electrophoresis with subsequent analysis of proteins by mass spectrometry. Yeast strain Rhodotorula glutinis CCY 20-2-26 showed the best adaptation to stress factors, which was moreover accompanied by overproduction of carotenoids. This finding can be premise for next industrial production of carotenoids. In plant samples predominantly enzymes and metabolites involved in antioxidant response were studied.
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Anastacio, Amandine. "Etude du profil protéomique de follicules ovariens de souris à 3 différents stades de développement in vitro." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2014. http://tel.archives-ouvertes.fr/tel-00990894.

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Alors que le protéome de l'ovocyte isolé, aux stades VG et MII a déjà été étudié, celui du follicule en croissance n'a jamais été décrit.Dans cette étude, nous avons cherché à identifier, comparer et caractériser les profils protéiques de follicules ovariens de souris à trois stades de leur développement in vitro distincts morphologiquement : follicules secondaires en début de culture - stade initial (IS), follicules avec une rupture complète de la membrane de Slavjanski (RMS) et follicules avec une cavité similaire à l'antrum (FA).Après un préfractionnement par IEF et une analyse LC-MS/MS en deux configurations (1D et 2D), 1403 protéines ont pu être identifiées dans le follicule ovarien de souris. 43,4 % (609) des protéines identifiées étaient communes aux trois stades et d'autres ont été identifiées uniquement à un stade : 71 au stade IS, 182 au stade RMS et 193 au stade FA. De plus, on a identifié 365 protéines qui n'avaient pas été décrites antérieurement dans le protéome de l'ovocyte ce qui pourrais indiquer qu'elles sont exprimées dans les cellules somatiques du follicule. Des analyses qualitatives et quantitatives complémentaires ont démontré une surreprésentation pour 44 fonctions biologiques par rapport aux fonctions biologiques des gènes constituant le génome de Mus musculus et mis en évidence des différences d'expression et d'abondance des protéines liées au cycle cellulaire, à la fixation des ions de calcium et à la glycolyse selon le stade de développement. Ces résultats représentent un point de départ pour beaucoup d'autres études de caractérisation moléculaire du développement folliculaire.
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Ngara, Rudo. "A proteomic analysis of drought and salt stress responsive proteins of different sorghum varieties." Thesis, University of the Western Cape, 2009. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1434_1334579378.

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This study reports on a proteomic analysis of sorghum proteomes in response to salt and hyperosmotic stresses. Two-dimensional gel electrophoresis (2DE) in combination with mass spectrometry (MS) was used to separate, visualise and identify sorghum proteins using both sorghum cell suspension cultures and whole plants. The sorghum cell suspension culture system was used as a source of culture filtrate (CF) proteins. Of the 25 visualised CBB stained CF spots, 15 abundant and well-resolved spots were selected for identification using a combination of MALDI-TOF and MALDI-TOFTOF MS, and database searching. Of these spots, 14 were positively identified as peroxidases, germin proteins, oxalate oxidases and alpha-galactosidases with known functions in signalling processes, defense mechanisms and cell wall metabolism.

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Books on the topic "2D MS/MS"

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TRICAD® MS: Grundlagen und Anwendungen anhand der Module Lüftung 2D und 3D. Wiesbaden: Vieweg+Teubner Verlag, 2004.

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Book chapters on the topic "2D MS/MS"

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Messmer, Harald. "Toolbox Konstruktionshilfen -2D." In TRICAD® MS, 52–69. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_4.

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Messmer, Harald. "Menü Platzieren -2D." In TRICAD® MS, 70–87. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_5.

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Messmer, Harald. "Menü Platzieren -2D." In TRICAD® MS, 88–89. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_6.

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Messmer, Harald. "Menü Werkzeug -2D." In TRICAD® MS, 90–108. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_7.

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Messmer, Harald. "Menü Ändern -2D." In TRICAD® MS, 109–17. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_8.

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Messmer, Harald. "Menü Einstellungen -2D." In TRICAD® MS, 118–34. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_9.

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Messmer, Harald. "Einführung TRICAD MS -2D." In TRICAD® MS, 1–5. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_1.

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Messmer, Harald. "Toolbox Eckige Kanäle -2D." In TRICAD® MS, 5–31. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_2.

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Messmer, Harald. "Toolbox Runde Kanäle -2D." In TRICAD® MS, 32–51. Wiesbaden: Vieweg+Teubner Verlag, 2004. http://dx.doi.org/10.1007/978-3-322-92128-4_3.

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Weeks, Mark E. "Urinary Proteome Profiling Using 2D-DIGE and LC-MS/MS." In Methods in Molecular Biology, 293–309. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-780-8_18.

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Conference papers on the topic "2D MS/MS"

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Zhang, Yongqian, Chengjun Lai, Yong Zhu, and Yulin Deng. "Comprehensive Proteome Analysis of Human Smooth Muscle Cells by 2D-HPLC-ESI-MS/MS." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780052.

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Li, Xiu-Min. "Abstract LB-7: The high-risk subject screening of ESCC by iTRAQ-coupled 2D LC-MS/MS." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-lb-7.

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Cui, Zhiming, and Jaehyung Ju. "2D Motion Structures of N-Fold Rotational Symmetry." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-70854.

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Kinematic motion structures having a reconfigurable property appear to be a potential candidate for the programmable matter. Motion structures with N-fold rotational symmetry show a reconfigurable pattern transformation, resulting in providing tunable mechanical properties, which deserves to explore more for their unique properties of transformation and the corresponding structural behaviors. The objective of this work is to synthesize motion structures from a bar-and-joint framework, investigating their transformability, linear structural properties - modulus and Poisson’s ratio, and nonlinear structural behaviors with kinematic bifurcation. Two-dimensional (2D) motion structures are synthesized by central scissor links with revolute joints, connected with binary links in the radial direction. They possess an N-fold rotational symmetry (MS-N), and their transformed patterns are investigated. Five 2D motion structures — MS-4, MS-6, MS-8, MS-10, and MS-12, are generated for investigating their mechanical properties together with their transformability. Analytical models of the motion structures are constructed for obtaining relative density, moduli, Poisson’s ratios, volume at each transformed state, and the strain energy required to transform from one state to another. This study integrates kinematics and structural mechanics, expanding the design space of light-weight structural materials with pattern transformation.
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Fjeldskaar, W., J. Mykkeltveit, H. Johansen, J. M. Langfeldt, O. H. J. Christie, P. Tyvand, O. Skurve, and P. A. Bjørkum. "Interactive 2D Basin Modelling on Workstations." In Petroleum Computer Conference. Society of Petroleum Engineers, 1990. http://dx.doi.org/10.2118/20350-ms.

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Thompson, Troy, Jay Vogt, and Yan Zaretskiy. "Designing and Validating 2D Reservoir Models." In SPE Kingdom of Saudi Arabia Annual Technical Symposium and Exhibition. Society of Petroleum Engineers, 2017. http://dx.doi.org/10.2118/188066-ms.

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Virnovsky, G. A., and A. Lohne. "Efficient 2-phase upscaling in 2D." In SPE Asia Pacific Improved Oil Recovery Conference. Society of Petroleum Engineers, 2001. http://dx.doi.org/10.2118/72113-ms.

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Cao Minh, Chanh, Steven F. Crary, Lukasz Zielinski, Chengbing Liu, Sid Jones, and Scott James Jacobsen. "2D-NMR Applications in Unconventional Reservoirs." In SPE Canadian Unconventional Resources Conference. Society of Petroleum Engineers, 2012. http://dx.doi.org/10.2118/161578-ms.

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Gunn, Heather, Nathan Miller, Nathan Miller, Jay L. Banner, Jay L. Banner, Omero F. Orlandini, and Omero F. Orlandini. "USING LA-ICP-MS 2D CHEMICAL MAPPING OF STALAGMITE GROWTH FABRICS FOR SEASONALLY RESOLVED HYDROCLIMATE RECONSTRUCTION." In GSA Connects 2021 in Portland, Oregon. Geological Society of America, 2021. http://dx.doi.org/10.1130/abs/2021am-370208.

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Singer, Gabriela, Shouxiang Mark Ma, Songhua Chen, and Mahmoud Eid. "2D Surface Roughness Quantification for Enhanced Petrophysical Applications." In SPE Annual Technical Conference and Exhibition. SPE, 2022. http://dx.doi.org/10.2118/210178-ms.

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Abstract Surface roughness is an essential rock parameter affecting petrophysical properties that are surface sensitive such as characterization of pore structure and wettability. For instance, Wenzel's contact angle formula for rough surfaces requires knowledge of the surface roughness, and surface roughness is expected to speed up aging of cores in crude oil for wettability restoration. In addition, proper quantification of surface roughness is critical for obtaining representative, roughness-independent, pore sizes for applications such as prediction of permeability and interpretation of capillary pressure curves. Intuitively, a surface is better characterized in 2D than in 1D. This 2D study is a continuation and enhancement of the previous 1D work, recently published in the SPE Journal (Ma et al., 2021). In this current paper, a comprehensive investigation of 1D versus 2D surface roughness measurements is conducted to evaluate and cross validate the two approaches. In this study, surface roughness is measured on 26 carbonate rock samples by laser scanning confocal microscopy (LSCM), where both the 1D absolute increment surface roughness, Sr, as well as 2D interfacial area ratio of surface roughness, Sdr, are reported. As expected, results indicate that surface roughness characterized by 2D Sdr has a greater dynamic range than the 1D Sr measurement, i.e., the 2D Sdr provides a more representative characterization of surface roughness. A detailed account of methodologies, assumptions, limitations, validation and applications of the 1D and 2D surface roughness characterization is documented in this paper. To extract the roughness features present on rock grain surfaces, effects of de-spiking and filter length, used to eliminate pore size effects, are investigated. For specific applications of surface roughness corrected pore size estimation from nuclear magnetic resonance (NMR) measurements, differences in length-scales of surface roughness are compared between LSCM measurement and that derived from NMR diffusion-T2 plus BET surface area. The surface roughness-corrected NMR pore-size distribution is also validated against the pore-size distribution obtained from measurement of micro-CT scanning.
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Kikuchi, Shuichi. "2D and 3D Well Planning for Horizontal Wells." In Middle East Oil Show. Society of Petroleum Engineers, 1993. http://dx.doi.org/10.2118/25647-ms.

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Reports on the topic "2D MS/MS"

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McKnight, C., David May, and Keaton Jones. Numerical analysis of dike effects on the Mississippi River using a two-dimensional Adaptive Hydraulics model (AdH). Engineer Research and Development Center (U.S.), November 2022. http://dx.doi.org/10.21079/11681/46120.

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This report describes the hydraulic effects of dikes on water surface elevation (WSE) and velocities in the Mississippi River near Vicksburg, MS, from Interstate 20 to Highway 80 using a previously calibrated 2D Adaptive Hydraulics numerical model. Dike heights and their associated hydraulic roughness values were varied to quantify the overall effects of adjustments to dike fields. Steady flows characterized as low, medium, and high conditions were simulated. The WSE and velocity difference plots were generated to illustrate the hydraulic effects on the river under all scenarios discussed above. Overall, the dike adjustments had negligible impacts on WSEs and showed minimal effects on velocities on a system-wide scale.
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Heifetz, Yael, and Michael Bender. Success and failure in insect fertilization and reproduction - the role of the female accessory glands. United States Department of Agriculture, December 2006. http://dx.doi.org/10.32747/2006.7695586.bard.

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The research problem. Understanding of insect reproduction has been critical to the design of insect pest control strategies including disruptions of mate-finding, courtship and sperm transfer by male insects. It is well known that males transfer proteins to females during mating that profoundly affect female reproductive physiology, but little is known about the molecular basis of female mating response and no attempts have yet been made to interfere with female post-mating responses that directly bear on the efficacy of fertilization. The female reproductive tract provides a crucial environment for the events of fertilization yet thus far those events and the role of the female tract in influencing them are poorly understood. For this project, we have chosen to focus on the lower reproductive tract because it is the site of two processes critical to reproduction: sperm management (storage, maintenance, and release from storage) and fertilization. E,fforts during this project period centered on the elucidation of mating responses in the female lower reproductive tract The central goals of this project were: 1. To identify mating-responsive genes in the female lower reproductive tract using DNA microarray technology. 2. In parallel, to identify mating-responsive genes in these tissues using proteomic assays (2D gels and LC-MS/MS techniques). 3. To integrate proteomic and genomic analyses of reproductive tract gene expression to identify significant genes for functional analysis. Our main achievements were: 1. Identification of mating-responsive genes in the female lower reproductive tract. We identified 539 mating-responsive genes using genomic and proteomic approaches. This analysis revealed a shift from gene silencing to gene activation soon after mating and a peak in differential gene expression at 6 hours post-mating. In addition, comparison of the two datasets revealed an expression pattern consistent with the model that important reproductive proteins are pre-programmed for synthesis prior to mating. This work was published in Mack et al. (2006). Validation experiments using real-time PCR techniques suggest that microarray assays provide a conservativestimate of the true transcriptional activity in reproductive tissues. 2.lntegration of proteomics and genomics data sets. We compared the expression profiles from DNA microarray data with the proteins identified in our proteomic experiments. Although comparing the two data sets poses analyical challenges, it provides a more complete view of gene expression as well as insights into how specific genes may be regulated. This work was published in Mack et al. (2006). 3. Development of primary reproductive tract cell cultures. We developed primary cell cultures of dispersed reproductive tract cell types and determined conditions for organ culture of the entire reproductive tract. This work will allow us to rapidly screen mating-responsive genes for a variety of reproductive-tract specifi c functions. Scientific and agricultural significance. Together, these studies have defined the genetic response to mating in a part of the female reproductive tract that is critical for successful fertllization and have identified alarge set of mating-responsive genes. This work is the first to combine both genomic and proteomic approaches in determining female mating response in these tissues and has provided important insights into insect reproductive behavior.
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