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Academic literature on the topic '2D gel'

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Published: 4 June 2021
Last updated: 4 February 2022

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Journal articles on the topic "2D gel"

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Görg, Angelika. "Advances in 2D gel techniques." Trends in Biotechnology 18 (July 2000): 3–6. http://dx.doi.org/10.1016/s0167-7799(00)00011-1.

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Kaczmarek, K., B. Walczak, S. de Jong, and B. G. M. Vandeginste. "Matching 2D Gel Electrophoresis Images." Journal of Chemical Information and Computer Sciences 43, no. 3 (May 2003): 978–86. http://dx.doi.org/10.1021/ci0256337.

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RASHWAN, SHAMS, AMANY SARHAN, MUHAMMAD TALAAT FAHEEM, and BAYUMY A. YOUSSEF. "AUTOMATIC PROTEIN SPOTS QUANTIFICATION IN TWO-DIMENSIONAL GEL IMAGES." Advances in Adaptive Data Analysis 03, no. 03 (July 2011): 401–15. http://dx.doi.org/10.1142/s1793536911000726.

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Two-dimensional (2D) polyacrylamide gel electrophoresis of proteins is a robust and reproducible technique. It is the most widely used separation tool in proteomics. Current efforts in the field are directed at development of tools for expanding the range of proteins accessible with 2D gels. Proteomics was built around the 2D gel. The idea that multiple proteins can be analyzed in parallel grew from 2D gel maps. Proteomics researchers needed to identify interested protein spots by examining the gel. This is time-consuming, labor-extensive, and error-prone process. It is desired that the computer can analyze the proteins automatically by first detecting then quantifying the protein spots in the 2D gel images. In our previous work, we presented a new technique for segmentation of 2D gel images using the fuzzy c-means (FCM) algorithm using the notion of fuzzy relations. In this paper, we will describe the new relational FCM (RFCM) algorithm and use it for automatic protein spots quantification. We will also use two methods to evaluate its performance: the unsupervised evaluation method and comparison with the expert spots quantification.
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Winz, M. L., K. Rohr, and S. Wörz. "Geometric Alignment of 2D Gel Electrophoresis Images." Methods of Information in Medicine 48, no. 04 (2009): 320–23. http://dx.doi.org/10.3414/me9229.

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Summary Objectives: 2D gel electrophoresis (2-DE) is the method of choice for analyzing protein expression in the field of proteomics, for example, comparing a reference with a test population. However, due to complex physical and chemical processes the locations of proteins generally vary in different 2-DE images. To cope with these variations, accurate geometric alignment of 2-DE images is important. Methods: We introduce a new elastic registration approach for 2-DE images, which is based on an analytic solution of the Navier equation using Gaussian elastic body splines (GEBS). With this approach cross-effects in elastic deformations can be handled, which is important for the registration of 2-DE images. In addition, landmark correspondences can be included to aid the registration in regions which are difficult to register using intensity information alone. Results: We have successfully applied our approach to register 2-DE gel images of different levels of complexity. In each case, gel images from a reference group are compared with a test group. To analyze the performance of our approach, we have carried out a quantitative evaluation of the registration results. Moreover, we have performed an experimental comparison with a previous elastic registration scheme. Conclusions: From the results we found that our approach is well-suited for the registration of 2-DE gel images of different levels of complexity and it turned out that the approach is superior to a previous hybrid scheme. Moreover, our approach is well-suited in a fully automatic setting and the performance can further be improved when landmark correspondences are available.
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Bonchev, Danail, and Milan Randic. "Shannon’s entropy of proteomic 2D-gel maps." Chemical Physics Letters 372, no. 3-4 (April 2003): 548–52. http://dx.doi.org/10.1016/s0009-2614(03)00441-x.

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Johnson, D. Thor, Robert A. Harris, Stephanie French, Angel Aponte, and Robert S. Balaban. "Proteomic changes associated with diabetes in the BB-DP rat." American Journal of Physiology-Endocrinology and Metabolism 296, no. 3 (March 2009): E422—E432. http://dx.doi.org/10.1152/ajpendo.90352.2008.

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These studies were structured with the aim of utilizing emerging technologies in two-dimensional (2D) gel electrophoresis and mass spectrometry to evaluate protein expression changes associated with type 1 diabetes. We reasoned that a broad examination of diabetic tissues at the protein level might open up novel avenues of investigation of the metabolic and signaling pathways that are adversely affected in type 1 diabetes. This study compared the protein expression of the liver, heart, and skeletal muscle of diabetes-prone rats and matched control rats by semiquantitative liquid chromatography-mass spectrometry and differential in-gel 2D gel electrophoresis. Differential expression of 341 proteins in liver, 43 in heart, and 9 (2D gel only) in skeletal muscle was detected. These data were assembled into the relevant metabolic pathways affected primarily in liver. Multiple covalent modifications were also apparent in 2D gel analysis. Several new hypotheses were generated by these data, including mechanisms of net cytosolic protein oxidation, formaldehyde generation by the methionine cycle, and inhibition of carbon substrate oxidation via reduction in citrate synthase and short-chain acyl-CoA dehydrogenase.
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Ji, H. "Preparation of Eukaryotic Lysates for 2D Gel Electrophoresis." Cold Spring Harbor Protocols 2006, no. 28 (October 1, 2006): pdb.prot4571. http://dx.doi.org/10.1101/pdb.prot4571.

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Liang, C., and S. A. Gerbi. "Analysis of an origin of DNA amplification in Sciara coprophila by a novel three-dimensional gel method." Molecular and Cellular Biology 14, no. 2 (February 1994): 1520–29. http://dx.doi.org/10.1128/mcb.14.2.1520.

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The replication origin region for DNA amplification in Sciara coprophila DNA puff II/9A was analyzed with a novel three-dimensional (3D) gel method. Our 3D gel method involves running a neutral/neutral 2D gel and then cutting out vertical gel slices from the area containing replication intermediates, rotating these slices 90 degrees to form the third dimension, and running an alkaline gel for each of the slices. Therefore, replication intermediates are separated into forks and bubbles and then are resolved into parental and nascent strands. We used this technique to determine the size of forks and bubbles and to confirm the location of the major initiation region previously mapped by 2D gels to a 1-kb region. Furthermore, our 3D gel analyses suggest that only one initiation event in the origin region occurs on a single DNA molecule and that the fork arc in the composite fork-plus-bubble pattern in neutral/neutral 2D gels does not result from broken bubbles.
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Liang, C., and S. A. Gerbi. "Analysis of an origin of DNA amplification in Sciara coprophila by a novel three-dimensional gel method." Molecular and Cellular Biology 14, no. 2 (February 1994): 1520–29. http://dx.doi.org/10.1128/mcb.14.2.1520-1529.1994.

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The replication origin region for DNA amplification in Sciara coprophila DNA puff II/9A was analyzed with a novel three-dimensional (3D) gel method. Our 3D gel method involves running a neutral/neutral 2D gel and then cutting out vertical gel slices from the area containing replication intermediates, rotating these slices 90 degrees to form the third dimension, and running an alkaline gel for each of the slices. Therefore, replication intermediates are separated into forks and bubbles and then are resolved into parental and nascent strands. We used this technique to determine the size of forks and bubbles and to confirm the location of the major initiation region previously mapped by 2D gels to a 1-kb region. Furthermore, our 3D gel analyses suggest that only one initiation event in the origin region occurs on a single DNA molecule and that the fork arc in the composite fork-plus-bubble pattern in neutral/neutral 2D gels does not result from broken bubbles.
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Natale, Massimo, Bernardetta Maresca, Paolo Abrescia, and Enrico M. Bucci. "Image Analysis Workflow for 2-D Electrophoresis Gels Based on ImageJ." Proteomics Insights 4 (January 2011): PRI.S7971. http://dx.doi.org/10.4137/pri.s7971.

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A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow, have prompted several groups to develop freeware systems to perform certain steps of gel analysis. Unfortunately, to the best of our knowledge none of them offer a package that performs all the steps envisaged in a 2D-GE gel analysis. Here we describe an ImageJ-based procedure, able to manage all the steps of a 2D-GE gel analysis. ImageJ is a free available image processing and analysis application developed by National Institutes of Health (NIH) and widely used in different life sciences fields as medical imaging, microscopy, western blotting and PAGE. Nevertheless no one has yet developed a procedure enabled to compare spots on 2D-GE gels. We collected all used ImageJ tools in a plug-in that allows us to perform the whole 2D-GE analysis. To test it, we performed a set of 2D-GE experiments on plasma samples from 9 patients victims of acute myocardial infarction and 8 controls, and we compared the results obtained by our procedure to those obtained using a widely diffuse commercial package, finding similar performances.
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Dissertations / Theses on the topic "2D gel"

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Van, Phu T. "Improving 2D Gel Proteomics With The Structured Illumination Gel Imager." Research Showcase @ CMU, 2014. http://repository.cmu.edu/dissertations/365.

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This thesis is composed of three separate projects: 1. Proteomics is the study of complex protein mixtures found in a cell, organ, or entire organism. The vast concentration range of these samples, estimated at approximately 150,000-fold for simple unicellular eukaryotes is beyond current detection methods. We present a technology called Structured Illumination (SI) Gel Imager that employs an LCD projector to selectively illuminate fluorescently labeled proteins separated into individual protein spots on 2-dimensional electrophoresis (2DE) gels. SI Gel Imager images have a dynamic range of approximately 1,000,000-fold, making it a valuable tool for proteomic detection. 2. 2DE gels possess the ability to separate proteins with extremely high resolution of molecular-weight and isoelectric-point. However, they suffer from variable sample loss incurred during protein reduction and alkylation steps required for subsequent sequencing by mass-spectrometry, up to about 30% of the starting sample. We present a protein equilibration method utilizing agarose stacking gels to reduce experiment variability and sample loss. 3. 2DE-based proteomics is a time-consuming process, requiring up to 3 days, and suffers from low reproducibility. To provide undergraduates to the experience of conducting proteomics research, we developed the Proteomics Platoon approach, where a group of undergraduate students work in two-person teams to perform proteomic experiments using a wide variety of biological samples. The close-knit nature of the Platoon further fosters collaboration, communication and mentorship while completing complex scientific projects. The Platoon approach serves as a model for involving undergraduates in complex research projects.
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Malak, Ramez. "2D gel analysis on CNP-overexpressing mouse brain." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79041.

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2',3'-Cyclic nucleotide 3 '-phosphodiesterase (CNP) is an early marker for oligodendrocytes, and it is suspected to be implicated in the expansion of membranes during myelination. We have previously generated transgenic mice that overexpress CNP. These mice showed altered oligodendroyte development, produced aberrant myelination, and had less MBP accumulated in myelin. More interestingly, ODCs isolated from those mice had a tremendous increase in the process extension formation. The purpose of the present study is to compare the protein expression pattern in the myelin isolated from control and CNP overexpressing mice (L191), using two-dimensional gel electrophoresis. We found that CNP overexpression increases HSC70, and HSP70, and decreases MAG expression in myelin. We also found that the mRNA for MAG, in L191 brain was identical to control brain during all stages of development. These findings suggest that CNP may be implicated with HSC70 in vesicular transport, and this may explain the mechanism of process extension mediated by CNP.
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Chan, Hong-Lin. "A 2D-difference gel electrophoresis strategy for redox proteomics." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1444604/.

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Post-genomic biomedical science requires quantitative proteomics. In most cases this involves differential protein expression analysis using matched pairs of simultaneously detectable labelling reagents for specific protein amino acids. In this thesis the development and optimisation of a novel cysteine labelling strategy, that is based on the use of iodoacetyl derivatives of Cy3 and Cy5 (ICy3/5) and 2D-difference gel electrophoresis (2D-DIGE) is described. The differentially labelled samples are separated on a single 2D gel and detected by multi-wavelength fluorescence scanning. The method is used to analyse standard proteins and then cell lysates to define the stoichiometry, sensitivity and specificity of this labelling technique. A comparative study of this new proteomic ICy dye protocol with the current NHS-Cy dye labelling system and methods that employ commonly used protein staining methods is described. The method is then used for cysteine labelling of proteins in non-reduced, denatured biological samples allowing accurate monitoring and sensitive detection of redox-dependent thiol modifications and expression level changes. The method is shown to be compatible with the use of MALDI mass spectrometry to identify proteins by analysis of trypsinised ICy labelled peptide digests. Using parallel sample analysis within single gels, the ICy-dye reagents were used to detect redox-, ErbB-2- and growth factor-dependent changes in a human mammary luminal epithelial cell system which was exposed to hydrogen peroxide or to growth factor stimulation. The conventional lysine labelling 2D-DIGE technique was also used in parallel to assess the new ICy labelling strategy for determination of the effects of oxidative stress on protein isoform levels. This study has revealed the identity of proteins involved in the response to oxidative stress and growth factor stimulation in the context of ErbB-2 growth factor receptor over-expression. In addition, this labelling strategy was also used to detect changes in thiol reactivity that follow the UV irradiation of plasma proteins as part of a study designed to evaluate the effect of UV disinfection on plasma product safety for clinical use.
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Brown, Andrew S. "Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin." Youngstown State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768.

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Nadaf, Somayyeh. "Analyse protéomique et transcriptomique de la maturation folliculaire." Thesis, Tours, 2010. http://www.theses.fr/2010TOUR4037.

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La compréhension des mécanismes cellulaires et moléculaires qui sont mis en jeu au moment de la croissance et la maturation pré-ovulatoire induite par la LH, permettra de définir des marqueurs de qualité et de maturité du follicule destiné à ovuler, et ainsi de mieux anticiper le moment de l’ovulation. L’objectif majeur de cette thèse était d’identifier certain des facteurs régulateur impliqués dans la maturation folliculaire par deux approches globales d’analyse protéomique et transcriptomique. La première étude a permis d’établir pour la première fois les cartes protéiques du liquide folliculaire équin et canin. Les analyses comparatives des liquides folliculaires provenant de différents stades physiologiques n’ont montré, dans nos conditions expérimentales, que peu de différences. Nos résultats obtenus dans la deuxième étude indiquent que les différentes méthodes enrichissement du liquide folliculaire peuvent améliorer, pour certaines de manière conséquente, la résolution des gels 2D-PAGE. Notre étude transcriptomique globale a révélé un groupe de gènes différentiellement exprimés dans les cellules folliculaires aux différents stades étudiés. Ces gènes sont potentiellement impliqués pendant le développement folliculaire dans l’espèce équine. Les deux approches (protéomique et transcriptomique) que nous avons utilisées au cours de ce travail sont complémentaires car la connaissance des gènes exprimés par les cellules folliculaires peuvent permettre d’identifier certains gènes codant pour des protéines sécrétoires retrouvées dans le liquide folliculaire
An understanding of the cellular and molecular mechanisms involved in the growth and maturation of the preovulatory follicle induced by LH, will help us to understand and identify the markers of quality and maturity of the follicle destined to ovulate, and better anticipate the time of ovulation. The main objective of this thesis was to identify some regulatory factors involved in follicle maturation using two global approaches: proteomic and transcriptomic analysis. The first study established for the first time the protein map of equine and canine follicular fluids. The comparative analyses of follicular fluid from different physiological stages were shown little or no difference in our experimental conditions. Results obtained with the second study suggested that between depletion and enrichement methods, the enriched follicular fluid can improve for some consistent manner, the resolution of 2D-PAGE gels. Our global transcriptomic study revealed a group of genes differentially expressed in follicular cells at different physiological stages. These genes are potentially involved during follicle development in the equine species. The two approaches (proteomic and transcriptomic) that we used in this work are complementary, as the knowledge of genes expressed by follicle cells can help to identify some genes coding for secretory proteins secreted in follicular fluid
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Flesinski, Lionel. "Étude de la stabilité des émulsions et de la rhéologie interfaciale des systèmes pétrole brut/eau : influence des asphaltènes et des acides naphténiques." Thesis, Pau, 2011. http://www.theses.fr/2011PAUU3022/document.

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La formation d'émulsions eau-dans-huile stables est un problème majeur rencontré par les pétroliers au niveau de la production mais aussi du raffinage. Afin d'essayer de prévoir ce phénomène, Total a développé une méthode de classement des huiles qui permet, à partir de leurs propriétés physico-chimiques, de déterminer a priori leur capacité à former ou non des émulsions stables. Cependant, les mécanismes interfaciaux sous-jacents ainsi que l’influence des molécules tensioactives du brut sur la stabilité des émulsions n’est pas très clair. Notre travail a consisté à étudier la contribution des acides naphténiques et des asphaltènes dans les phénomènes observés. L’étude d’huiles réelles a permis d’établir un lien entre la stabilité des émulsions et la formation à l'interface d'un gel 2D. Les études menées sur les huiles réelles dont les acides naphténiques ont été extraits ont permis de montrer que ces derniers, en compagnie de leurs formes ionisées, les naphténates, ont la capacité de réduire la stabilité des émulsions en diminuant la résistance du gel interfacial, ou même en empêchant sa formation. Les expériences réalisées sur les huiles réelles dépourvues d’asphaltènes ont permis de confirmer le rôle stabilisant des asphaltènes. Les résultats obtenus suggèrent que les asphaltènes s’adsorbent sur le gel 2D déjà formé par des tensioactifs passés de l’huile vers l’eau et le rapprochent ainsi de sa transition vitreuse. La résistance du gel interfacial s'en trouve alors augmentée, ce qui conduit à la formation d'émulsions plus stables. En croisant le classement industriel des bruts opéré par Total et les résultats de l’étude, un mécanisme global, régi par la compétition entre les acides naphténiques, les naphténates et les asphaltènes à l’interface E/H est proposé pour expliquer les différences de stabilité observées avec les différentes huiles. Lorsque les acides et les naphténates sont suffisamment concentrés, ils empêchent la formation du gel interfacial et les émulsions sont peu stables. Lorsqu’ils sont moins concentrés le rôle des asphaltènes peut alors devenir prépondérant en donnant une cohésion plus importante au gel qui se rapproche de sa transition vitreuse, ce qui conduit en général au renforcement de la stabilité des émulsions formées
Water-in-crude oil emulsions are a major issue for oil companies in both production and refining facilities. Thanks to physical and chemical characterizations, Total set a classification which allows the decision of a crude oil ability to create stable emulsions. However the interfacial mechanisms implied and the influence of the indigenous surfactants of crude oil remain unclear. Our work consists in studying the naphthenic acids and asphaltenes contribution to the w/o emulsion stability. The study of realistic crude oils enabled the discovery of a link between the emulsion stability with the formation of a very particular interfacial behavior: a two-imensional gel. Experiments with desacidified oils have proven the destabilizing ability of naphthenic acids and their ionized form, naphthenates. They actually decrease the interfacial gel strength and can even prevent the gel formation. Asphaltenes-free crude oils have permitted to confirm the stabilizing role of asphaltenes. Rather than adsorbing directly on the interface, asphaltenes seem to adsorb on the interfacial gel already formed. The gel strength is thus increased and lead to higher emulsion stability. Thanks to these results and the industrial classification of crude oil developed by Total, a global mechanism explaining the emulsion stability process has been proposed. This mechanism is governed by the competition between asphaltenes, naphthenates and naphthenic acids at the water/oil interface. If the concentration of naphthenic acids and naphthenates is high enough, the interfacial gel cannot be formed and the emulsions are unstable. If the crude oil is not acidic enough, the asphaltenes influence increases dramatically and implies the strengthening of the gel which becomes closer to his glass transition. This generally leads to the formation of more stable emulsions
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Sutrisno, Hari. "Synthèse et caractérisation d'oxyde de titane (TiO2) micro-mésostructuré à dimensionnalité contrôlée (0D, 1D, 2D et 3D)." Nantes, 2001. http://www.theses.fr/2001NANT2098.

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Depuis ces dernières années, la chimie du titane et plus particulièrement celle du dioxyde (TiO2) fait l'objet d'une grande activité de recherche tant sur le plan fondamental qu'appliqué. Les principales applications concernent, en effet, des thématiques associées aux problèmes de protection de l'environnement qui ont pour objectifs la purification de l'air et la dépollution de l'eau à travers la photocatalyse. Dans de telles applications, les performances de TiO2 pourraient être optimisées par un contrôle spécifique, à l'échelle micro- ou mésostructurale, de la morphologie du matériau. La nano-structuration de TiO2 peut s'avérer également très intéressante dans la réalisation de membranes pour la séparation des gaz, de matériaux d'électrode nanoporeux pour le développement de nouveaux types de cellules solaires, et comme couches actives dans la conception de systèmes électrochromes. . .
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Pandey, Archana. "Proteome analysis of Pseudomonas putida KT2440 using 2D gel electrophoresis and LC/ESI-Q-TOF mass spectrometry /." Online version of thesis, 2007. https://ritdml.rit.edu/dspace/handle/1850/3848.

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Šopíková, Martina. "Změny proteinového profilu v průběhu sladování ječmene." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216437.

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This diploma thesis is focused on studies of changing of protein profile during barley malting. Substantial part of this work is devoted to the proteomics identification of barley proteins which change during malting and so become more stationary and they influence quality of beer (haze and foam in beer). For this experiment was used barley variety Jersey. In the theoretical part of this thesis there is information about beer, manufacturing of beer with description of important commodities for manufacturing of beer and information about barley malting and information about malting process. Next there is description of methods for separation of proteins (1D gel electrophoresis and 2D gel electrophoresis), MALDI TOF/TOF mass spectrometry and this use for the analysis and identification of proteins, the use of matrices and ways of the sample preparation. In the experimental part of this thesis there was carried out the optimisation of the dosage of sample for 1D gel electrophoresis and the optimisation of staining. The 15 % TRIS-HCl gel was the best, this gel was stained by Commassie Brilliant Blue G-250. For illustration of changes was made 2D gel electrophoresis. With help of method peptide mass fingerprinting and MS/MS protein of barley – protein Z, -amylase subtilisin inhibitor, -amylase a peroxidase were identificated. The analysis of barley extract intact proteins was carried out, this analysis was focused on changes of important barley protein LTP 1.
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Guterres, Sheila Barreto. "Busca de biomarcadores para esquizofrenia em plaquetas utilizando eletroforese diferencial em gel bidimensional (2D-DIGE) e espectrometria de massas." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-16112011-150931/.

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A esquizofrenia é uma doença crônica, grave e incapacitante que afeta cerca 24 milhões de pessoas em âmbito mundial. É caracterizada por uma desorganização no pensamento que prejudica a funcionalidade do indivíduo. Existem intervenções que são efetivas e contribuem para a diminuição da prevalência do transtorno, pois ajudam o portador a levar uma vida produtiva e integrada à sociedade, porém devem ser ministradas nos estágios iniciais da doença. No entanto, existe uma grande dificuldade em se diagnosticar a esquizofrenia precocemente devido a sua complexidade e às sutilezas dos seus sintomas apresentados antes do surgimento da psicose. O cérebro não é acessível a exames invasivos in vivo e por esse motivo a exploração de fluidos periféricos é de grande importância. As plaquetas e neurônios serotonérgicos possuem características bioquímicas e morfológicas em comum que possibilitam a comparação entre a estrutura e a função de ambos e, por causa dessa similaridade, muitos trabalhos utilizam plaquetas como modelo para o estudo de doenças neuropsiquiátricas, inclusive a esquizofrenia. A detecção precoce da esquizofrenia é um objeto de investigação atual e relevante não somente para revolucionar os meios atuais de diagnóstico, mas também para desenvolver novos tratamentos aplicados aos estágios iniciais da doença, diferenciar os subgrupos de doentes e monitorar as intervenções preventivas. A proposta do presente trabalho é fazer o estudo da expressão de proteínas em plaquetas de pacientes esquizofrênicos e controles com o objetivo de identificar proteínas candidatas a biomarcadores utilizando técnicas proteômicas quantitativas e confiáveis, como 2D-DIGE e a espectrometria de massas.
Schizophrenia is a disabling, serious, and chronic illness, which affects about 24 million people worldwide. It is characterized by a severe disorganization of the thoughts that harms the social life of patients becoming them dependent of the family and/or government. There are effective treatments that contribute to decrease the prevalence of the disorder because they improve the life and social conditions of the patients, but they are only advantageous if the intervention is made in the early stages of the disease. It is difficult to obtain early diagnosis due to the complexity of the disease and its insidious symptoms before the beginning of the psychosis. The brain is not easily accessed in vivo and, because of this, it is very important to study the peripheral tissues like blood, which makes the use of the platelets very interesting. Furthermore, platelets and serotonergic neurons share biochemical and morphological characteristics that allows the comparison between structure and function of both. From these similarities many authors has used platelets as a neuron model to study many neurodegenerative diseases including schizophrenia. The early detection of schizophrenia is a current and suitable goal, not only to improve the early diagnosis but also to develop new treatments, differentiate the subtypes, and monitor the preventive interventions. The purpose of this project is to do a comparative screening of expressed proteins in platelets from schizophrenics and controls with the objective of finding differently expressed proteins that could be candidates to biomarkers using 2D-DIGE and mass spectrometry.
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Books on the topic "2D gel"

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Anton, Posch, ed. 2D PAGE: Sample preparation and fractionation. Totowa, NJ: Humana Press, 2008.

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2D PAGE: Sample Preparation and Fractionation: Volume 1 (Methods in Molecular Biology). Humana Press, 2008.

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Posch, Anton. 2D PAGE: Sample Preparation and Fractionation: Volume 2 (Methods in Molecular Biology). Humana Press, 2008.

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Book chapters on the topic "2D gel"

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Kim, Helen, Shannon Eliuk, Jessy Deshane, Sreelatha Meleth, Todd Sanderson, Anita Pinner, Gloria Robinson, Landon Wilson, Marion Kirk, and Stephen Barnes. "2D Gel Proteomics." In Methods in Molecular Biology, 349–91. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-361-5_24.

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Simula, Maria Paola, Agata Notarpietro, Giuseppe Toffoli, and Valli De Re. "2-D Gel Electrophoresis: Constructing 2D-Gel Proteome Reference Maps." In Methods in Molecular Biology, 163–73. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-61779-424-7_13.

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Meleady, Paula. "Two-Dimensional Gel Electrophoresis and 2D-DIGE." In Methods in Molecular Biology, 3–14. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7268-5_1.

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Naula, Christina, and Richard Burchmore. "2D Gel Electrophoresis Analysis of Leishmania Proteomes." In Methods in Molecular Biology, 577–86. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0294-2_34.

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Xia, Xuhua. "Bioinformatics and In Silico 2D Gel Electrophoresis." In Bioinformatics and the Cell, 413–20. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-90684-3_18.

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Vormbrock, Ingo, Sonja Hartwig, and Stefan Lehr. "Multifluorescence 2D Gel Imaging and Image Analysis." In Methods in Molecular Biology, 21–30. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-573-2_3.

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Freeman, Lita A. "Native–Native 2D Gel Electrophoresis for HDL Subpopulation Analysis." In Methods in Molecular Biology, 353–67. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-60327-369-5_17.

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Rabilloud, Thierry. "How to Use 2D Gel Electrophoresis in Plant Proteomics." In Methods in Molecular Biology, 43–50. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-631-3_4.

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Marczyk, Michal. "Processing 2D Gel Electrophoresis Images for Efficient Gaussian Mixture Modeling." In Advances in Intelligent Systems and Computing, 35–42. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-60816-7_5.

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Vandekerckhove, J., M. Rider, H. H. Rasmussen, S. De Boeck, M. Puype, J. Van Damme, B. Gesser, and J. Celis. "Routine Amino Acid Sequencing on 2D-Gel Separated Proteins: A Protein Elution and Concentration Gel System." In Methods in Protein Sequence Analysis, 11–19. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4899-1603-7_2.

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Conference papers on the topic "2D gel"

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ceccarelli, Michele, and Jens Michael Carstensen. "Bayesian Grid matching for 2D gel registration." In 2010 IEEE International Conference on Imaging Systems and Techniques (IST). IEEE, 2010. http://dx.doi.org/10.1109/ist.2010.5548506.

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Wang, Weixing. "Spot identification on 2D electrophoresis gel images." In Fourth International Conference on Photonics and Imaging in Biology and Medicine, edited by Kexin Xu, Qingming Luo, Da Xing, Alexander V. Priezzhev, and Valery V. Tuchin. SPIE, 2006. http://dx.doi.org/10.1117/12.710884.

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Kostopoulou, Eirini, Eleni Zacharia, and Dimitris Maroulis. "Detection and segmentation in 2D gel electrophoresis images." In 2011 17th International Conference on Digital Signal Processing (DSP). IEEE, 2011. http://dx.doi.org/10.1109/icdsp.2011.6004967.

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Zacharia, Eleni, Eirini Kostopoulou, Dimitris Maroulis, and Sophia Kossida. "A Spot Segmentation Approach for 2D Gel Electrophoresis Images Based on 2D Histograms." In 2010 20th International Conference on Pattern Recognition (ICPR). IEEE, 2010. http://dx.doi.org/10.1109/icpr.2010.622.

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Hoang, Minh-Tuan T., Hieu T. Huynh, Nguyen H. Vo, Yonggwan Won, and Jung-Ja Kim. "Two-Step Iterative Registration for 2D-Gel Electrophoresis Images." In 2007 IEEE International Conference on Research, Innovation and Vision for the Future. IEEE, 2007. http://dx.doi.org/10.1109/rivf.2007.369168.

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Tsakanikas, P., and I. Manolakos. "Effective Denoising of 2D Gel Proteomics Images using Contourlets." In 2007 IEEE International Conference on Image Processing. IEEE, 2007. http://dx.doi.org/10.1109/icip.2007.4379573.

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Desai, Shrinivas D., and S. D. Savitha. "Seeded Watershed Segmentation Based Proteomics for 2D-Gel Electrophoresis Images." In the Third International Symposium. New York, New York, USA: ACM Press, 2015. http://dx.doi.org/10.1145/2791405.2791449.

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Sahoo, Biswa Mohan, Ranjeet Kumar Rout, and Manoj Kumar Sah. "Morphological approach for detection of protein spots from 2D-gel image." In 2016 International Conference on Innovation and Challenges in Cyber Security (ICICCS-INBUSH). IEEE, 2016. http://dx.doi.org/10.1109/iciccs.2016.7542325.

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Kostopoulou, Eirini, Stamos Katsigiannis, and Dimitris Maroulis. "A custom grow-cut based scheme for 2D-gel image segmentation." In 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2015. http://dx.doi.org/10.1109/embc.2015.7318879.

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Hoeflich, Christopher S., and Jason J. Corso. "Segmentation of 2D gel electrophoresis spots using a Markov random field." In SPIE Medical Imaging, edited by Josien P. W. Pluim and Benoit M. Dawant. SPIE, 2009. http://dx.doi.org/10.1117/12.811802.

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Reports on the topic "2D gel"

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McBride, Cory L., Rodney Cannon Schmidt, Victor R. Yarberry, and Ray J. Meyers. GBL-2D Version 1.0: a 2D geometry boolean library. US: Sandia National Laboratories, November 2006. http://dx.doi.org/10.2172/899723.

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Hasell, Douglas K. New Approach for 2D Readout of GEM Detectors. Office of Scientific and Technical Information (OSTI), October 2011. http://dx.doi.org/10.2172/1030606.

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Lu, Tzu-Ming, Lisa A. Tracy, D. Laroche, S. H. Huang, Y. Chuang, Y. H. Su, J. Y. Li, and C. W. Liu. Quantum Hall ferromagnetic transition in a Ge 2D hole system. Office of Scientific and Technical Information (OSTI), December 2016. http://dx.doi.org/10.2172/1505345.

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Longacre, Ronald S. 2D Azimuthal Space for Au + Au Mid-Central Collisions at √sNN = 200 GeV. Office of Scientific and Technical Information (OSTI), October 2019. http://dx.doi.org/10.2172/1570677.

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