Dissertations / Theses on the topic '2 dimensional gel electrophoresis'
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Yao, Mingyi. "Proteomic studies of Pseudomonas putida KT2440 in carcinogenicity screening via 2-dimensional gel electrophoresis /." Online version of thesis, 2005. https://ritdml.rit.edu/dspace/handle/1850/1118.
Full textRajagopal, Meena Uma. "METHODS DEVELOPMENT AND APPLICATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS AND MASS SPECTROMETRY IN PROTEOMICS." UKnowledge, 2006. http://uknowledge.uky.edu/gradschool_diss/292.
Full textAnduri, Sridevi. "Differential protein expression profiles in normal and intersex male smallmouth bass determined using one- and two-dimensional gel electrophoresis a thesis presented to the faculty of the Graduate School, Tennessee Technological University /." Click to access online, 2009. http://proquest.umi.com/pqdweb?index=0&did=2000377671&SrchMode=1&sid=2&Fmt=6&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1277817194&clientId=28564.
Full textTrein, Cristina Rodrigues. "Eletroforese bidimensional em gel de poliocrilamida do plasma seminal equino e a sua relação com a congelabilidade do sêmen." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/39292.
Full textThe objective of this study was to evaluate protein profile of equine seminal plasma using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and to find if any of these proteins were related to semen freezability. Seminal plasma was collected from 10 stallions of high and low semen freezability routinely used in AI programs from the State Stud of Lower Saxony and Artificial Insemination Center. Twenty five protein spots were identified from the 2 D gel (12%), seven of which were present in all samples. Of the 25 proteins found in the research stallions, two spots showed superior relative content (P<0.05) in seminal plasma samples collected from stallions with high semen freezability: the spots 5 (80-85 kDa, pI 7.54) was identified as CRISP3 and 45 (18.2 kDa, pI 5.0-5.2) was identified as HSP-2. Conversely, the spot 7 (75.4 kDa, pI 6.9 – 7.4) was identified as lactoferrin, 15 (26.7 kDa, pI 5.51) was identified as kallikrein, 25 (25 kDa, pI 7.54) was identified as CRISP3 and 35 (13.9 kDa, pI 3.8 – 4.2) was identified as HSP-1, showed superior relative protein content (P<0.05) on seminal plasma samples from stallions with low semen freezability. The proteins were identified by MALDI-MS. There were differences in the seminal plasma protein profile between stallions with high and low semen freezability. It may be suggested that CRISP3 and HSP-2 may be considered possible seminal plasma markers of high semen freezability.
Misztal, David Richard Biotechnology & Biomolecular Sciences Faculty of Science UNSW. "Identification of cellular changes associated with increased production of human follicle stimulating hormone in a recombinant Chinese hamster ovary cell line." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2008. http://handle.unsw.edu.au/1959.4/41326.
Full textHadjem, Ammar Saïd. "Traitement d'image du gel d'électrophorèse bidimensionnelle." Nancy 1, 1988. http://www.theses.fr/1988NAN10143.
Full textCoaker, Gitta Laurel. "Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis." The Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1069188955.
Full textApraiz, Larrucea Itxaso. "Development and application of a proteomic approach to the assessment of pollution in the marine environment." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-26150.
Full textQiu, Linghua. "Differentially Expressed Proteins in the Pancreas of Diabetic Mice." Ohio University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1125866599.
Full textAmelina, Hanna. "Proteomics in biomarker research : Insights into the effects of aging and environment on biological systems." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-56483.
Full textAt the time of the doctoral defense, the following publications were unpublished and had a status as follows: Paper 3: Submitted, Paper 4: Submitted.
ARBA, MORENA. "Caratterizzazione proteomica di fluidi e tessuti in diverse condizioni fisio-patologiche." Doctoral thesis, Università degli Studi di Cagliari, 2015. http://hdl.handle.net/11584/266808.
Full textKierul, Kinga. "Comprehensive proteomic study of Bacillus amyloliquefaciens strain FZB42 and its response to plant root exudates." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16805.
Full textBacillus amyloliquefaciens strain FZB42 is a free-living bacterium that competitively colonizes plant roots and stimulates plant growth by many different modes of action. The molecular basis of singular beneficial effects that this Plant Growth-Promoting Rhizobacteria (PGPR) exert on their hosts have been studied. To decipher the molecular cross-talk of B. amyloliquefaciens and its’ host plants as a whole system, an extensive proteomic approach was performed. Reference maps of the extracellular and cytosolic protein fractions were established. The highest number of secreted proteins was observed during stationary growth phase. Identified extracellular proteins belong to different functional classes, with the most prominent classes involved in carbohydrate degradation and transportation of molecules across the cell wall. Cytosolic extracts obtained from cultures grown in 1C and minimal media subjected to the 2 Dimensional Electrophoresis (2 DE), revealed 461 and 245 different protein entries, respectively. Created reference maps were subsequently used to identify proteins and processes involved in the interaction with plants, prior to exposure of bacteria to maize (Zea mays L.) root exudates. The proteomics of two strains lacking expression of genes coding for global transcriptional regulators (degU, abrB) and four sigma factors (sigB, sigM, sigV, and sigX) were also inves-tigated, in order to analyse their involvement in bacterial responses to root exudates. In summary, this is the first study presenting comprehensive proteomics of Gram-positive PGPR, evaluating at the same time changes in protein expression caused by addition of root exudates at the extracellular and cytosolic level.
Rye, Morten Beck. "Image segmentation and multivariate analysis in two-dimensional gel electrophoresis." Doctoral thesis, Norwegian University of Science and Technology, Department of Chemistry, 2007. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-1744.
Full textThe topic of this thesis is data-analysis on images from two-dimensional electrophoretic gels. Because of the complexity of these images, there are numerous steps and approaches to such an analysis, and no “golden standard” has yet been established on how to produce the desired output. In this thesis focus is put on two essential fields concerning 2D-gel analysis; registration of images by segregation and protein spot identification, and data-analysis on the output of such a registration by multivariate methods. Image segmentation is mainly concerned with the task of identifying individual protein spots in a gel-image. This has generally been the natural starting point of all methods and procedures developed since the introduction of 2D-gels in the mid-seventies, simply because this best reproduces the results created by a human analyst, who manually identify protein-spot entities. The amount of data produced in a 2D-gel experiment can be quite large, especially in multiple gels where the human analyst is dependent on additional statistical data-analytical tools to produce results. Because of the correlated nature of most gel-data, analysis by multivariate methods is natural choice, and are therefore adopted in this thesis. The goal of this thesis is to introduce the above mentioned procedures at different stages in the analysis pipeline where they are not yet fully exploited, rather than to improve already existing algorithms. In this way new insight and ideas on how to handle data from 2D-gel experiments are achieved. The thesis starts with a review of segmentation methodology, and introduces a selected procedure used to identify protein spots throughout. Output from the segmentation is then used to create a multivariate spot-filtering model, which aims to separate protein spots from noise and artefacts often creating problems in 2D-gel analysis. Lately the use of common spot boundaries in multiple gels have been the method of choice when gels are analysed. How such boundaries should be defined is an important subject of discussion, and thus a new method for defining common boundaries based on the individual segmentation of each gel is introduced. Segmentation may be a natural starting point when gels are analysed, but it is not necessarily the most correct. Often the introduction of fixed spot entities introduces restrictions to the data which cause problems at later stages in the analysis. Analysing pixels from multiple gels directly has no such restrictions, and it is shown in this thesis that the output of such an analysis based on multivariate methods can produce very useful results. It can also give insight to the data problematic to achieve with the spot boundary approach. At last in the thesis an improved pixel-based approach is introduced, where a less restricted segmentation is used to reduce and concentrate the amount of data analysed, improving the final output.
Brown, Andrew S. "Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Characterization of Decorin." Youngstown State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311873768.
Full textXu, Aoshuang. "Development in electrophoresis instrumentation for two-dimensional gel electrophoresis of protein separation and application of capillary electrophoresis in micro-bioanalysis /." [Ames, Iowa : Iowa State University], 2008.
Find full textEthell, Douglas Wayne. "Analysis of developing chick Gallus domesticus spinal cord proteins using two dimensional gel electrophoresis." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29834.
Full textScience, Faculty of
Zoology, Department of
Graduate
Fowlkes, Kelly. "A proteomic study of Pseudomonas putida by two-dimensional gel electrophoresis : establishing quantitative standards for intra-laboratory results /." Online version of thesis, 2007. http://hdl.handle.net/1850/5040.
Full textPandey, Archana. "Proteome analysis of Pseudomonas putida KT2440 using 2D gel electrophoresis and LC/ESI-Q-TOF mass spectrometry /." Online version of thesis, 2007. https://ritdml.rit.edu/dspace/handle/1850/3848.
Full textSchwartz, Anne. "Characterization of normal and androgen resistant-genital skin fibroblasts using high-resolution two-dimensional gel electrophoresis." Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63378.
Full textLI, Jinxiang, Ayaka OGASAWARA, Tamao ODAKE, Tomonari UMEMURA, and Kin-ichi TSUNODA. "A New Isoelectric Focusing System for Fast Two-Dimensional Gel Electrophoresis Using a Low-Concentration Polyacrylamide Gel Supported by a Loose Multifilament String." 日本分析化学会, 2005. http://hdl.handle.net/2237/8750.
Full textHilson, Tiffany D. "Developing and Optimizing a Mass Spec-Friendly Protocol for Fractionation of tRNAs Through Two-Dimensional Polyacrylamide Gel Electrophoresis." University of Cincinnati / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1322050702.
Full textCarlsson, Anders. "Identification of potential plasma biomarkers of inflammation in farmers with musculoskeletal disorders : A proteomic study." Thesis, Linköpings universitet, Kemi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-110782.
Full textCharoensri, Nicha. "Investigation into genetically programmed responses to cadmium and mercury in HeLa cells by differential display and two-dimensional gel electrophoresis." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84491.
Full textSilva, Fábio Arlindo [UNESP]. "Selênio em tilápia do Nilo utilizando eletroforese em gel e espectrometria atômica." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/104086.
Full textFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Universidade Estadual Paulista (UNESP)
O presente trabalho teve como objetivo investigar a presença de selênio em spots protéicos de amostras de plasma, músculo e fígado de tilápia do Nilo (Oreochromis niloticus) obtidos após separação das proteínas por eletroforese em gel de poliacrilamida em segunda dimensão (2D-PAGE) e posterior avaliação qualitativa por fluorescência de raios-X com radiação síncrotron (SR-XRF). A análise dos espectros de fluorescência obtidos indicaram a presença de selênio em oito proteínas do plasma, seis proteínas do músculo e cinco proteínas do fígado. Observou-se que o selênio está distribuído em sua maioria em proteínas com massa molar menor que 50 kDa. Proteínas acima de 50 kDa foram encontradas somente no plasma.
An investigation was made into selenium in protein spots of samples of plasma, muscle and liver of Nile tilapia (Oreochromis niloticus) obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and subsequent qualitative evaluation by synchrotron radiation X-ray fluorescence (SR-XRF). An analysis of the fluorescence spectra indicated the presence of selenium in eight plasma proteins, six muscle proteins, and five liver proteins. Selenium was found to be distributed mainly in proteins with a molar mass smaller than 50 kDa. Proteins with a molar mass higher than 50 kDa was found only in the plasma.
Bartee, Eric Carter. "Discovery and characterization of a novel family of human ubiquitin ligases termed Membrane Associated RING-CH (MARCH) proteins." Oregon Health & Science University, 2007. http://content.ohsu.edu/u?/etd,629.
Full textMolecular Microbiology and Immunology
Both poxviruses and γ2-herpesviruses share the K3-family of viral immune evasion proteins. These proteins are characterized by an amino-terminal RING-CH domain followed by two transmembrane domains. We analyzed several human homologues of the K3-family termed membrane-associated RING-CH (MARCH) proteins. All MARCH proteins localized to subcellular membranes while several reduced surface levels of known K3-family substrates. Thus, MARCH proteins appear to be structurally and functionally homologous to viral K3 proteins. One of the major challenges in determining the function of this family is the identification of their physiological substrates. To overcome this we created a quantitative proteomics approach which can be used to identify novel substrates for both the K3- and MARCH-families. Using stable isotope labeling by amino acids in cell culture, we compared the proteome of plasma membrane, golgi, and endoplasmic reticulum membranes in the presence and absence of K5 and MARCH-VIII. Quantitative mass spectrometric protein identification from these fractions revealed that CD316 (bone marrow stromal antigen 2), CD166 (activated leukocyte cell adhesion molecule) and syntaxin-4 were consistently underrepresented in the plasma membrane of K5 expressing cells, while CD44, CD81 (TAPA-1) and B-cell receptor-associated protein 31kDa (Bap31) were consistently underrepresented in the plasma membrane of MARCH-VIII expressing cells. Furthermore, downregulation of each of these proteins was independently confirmed. Our results both identify and characterize a novel family of human ubiquitin ligase enzymes and elucidate a novel technique which can analyze this family and be easily adapted to the analysis of other cellular enzymes viral immune modulators.
Dennard, Rollin. "Proteomic variations between a Mycoplasma gallisepticum vaccine strain and a virulent field isolate." Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/biology_diss/99.
Full textDwyer, Virginia Michelle Gregory 1955. "A STUDY OF PINEAL GLAND POLYPEPTIDES AND PROTEINS BY POLYACRYLAMIDE GEL ISOELECTRIC FOCUSING (PAG-IEF) AND TWO-DIMENSIONAL ELECTROPHORESIS (2DE) (BRAIN REGIONS)." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/276560.
Full textFulton, Benjamin L. "2D-PAGE Analysis of Myocardial Collagen in Male and Female Spontaneously Hypertensive Rats." Connect to resource online, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1219668882.
Full textBadillo-Vargas, Ismael. "Dissecting the molecular interplay between tomato spotted wilt virus and the insect vector, Frankliniella occidentalis." Diss., Kansas State University, 2014. http://hdl.handle.net/2097/35045.
Full textDepartment of Plant Pathology
Anna E. Whitfield
The Bunyaviridae is a family of animal and plant viruses that pose a threat to human, animal, and plant health worldwide. In nature, the dissemination of these viruses is dependent on arthropod vectors (genera Orthobunyavirus, Nairovirus, Phlebovirus, and Tospovirus) or rodent vectors (genus Hantavirus). The genus Tospovirus is the only one within this virus family that is composed of plant-infecting viruses transmitted by thrips. Tomato spotted wilt virus (TSWV), the type species of the Tospovirus genus, is one of the ten most devastating plant viruses known. It is most efficiently transmitted by the western flower thrips, Frankliniella occidentalis Pergande, in a persistant propagative manner. The insect molecules associated with virus infection and transmission by the thrips vector remain unidentified to date. The aim of this work was to identify F. occidentalis larval thrips proteins that are differentially expressed during TSWV infection of the insect vector and those that directly interact with TSWV. To achieve these goals, I used two-dimensional (2-D) gel electrophoresis and mass spectrometry coupled with Mascot searches. I identified 26 protein spots that displayed differential abundances in response to TSWV infection, which contained 37 proteins. Sixty two percent of these proteins were down-regulated by the viral infection demonstrating a complex response. Moreover, 8 and 11 protein spots that directly interacted with purified TSWV virions and a TSWV glycoprotein (GN), respectively, were identified in overlay assays of larval thrips proteins resolved by 2-D gel electrophoresis. A total of five proteins were identified from these spots. These interacting proteins might play roles in attachment and entry, endocytosis/exocytosis, and escape from different tissues for transmission to occur. Injection of double-stranded RNA (dsRNA) into adult female thrips triggered an RNAi response that resulted in 23% reduction of the target gene transcript level. This significant reduction resulted in increased mortality and decreased fertility compared to insects injected with control dsRNA or water and non-injected insects as well. The work presented here provides new insights on the molecular basis of this virus-vector interaction and describes new tools to conduct functional genomic assays to study gene function and design control strategies of F. occidentalis.
Martin, Kerri Katherine. "Exploring Metallic Flavor Perception: Analysis of Human Salivary Proteins and the Use of the Iron-Binding Protein Lactoferrin in Reducing Metallic Off-Flavors." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/76813.
Full textMaster of Science in Life Sciences
Silva, Fábio Arlindo. "Selênio em tilápia do Nilo utilizando eletroforese em gel e espectrometria atômica /." Botucatu : [s.n.], 2009. http://hdl.handle.net/11449/104086.
Full textAbstract: An investigation was made into selenium in protein spots of samples of plasma, muscle and liver of Nile tilapia (Oreochromis niloticus) obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and subsequent qualitative evaluation by synchrotron radiation X-ray fluorescence (SR-XRF). An analysis of the fluorescence spectra indicated the presence of selenium in eight plasma proteins, six muscle proteins, and five liver proteins. Selenium was found to be distributed mainly in proteins with a molar mass smaller than 50 kDa. Proteins with a molar mass higher than 50 kDa was found only in the plasma.
Orientador: Pedro de Magalhães Padilha
Coorientador: Marco Aurélio Zezzi Arruda
Banca: Paulo Roberto Rdrigues Ramos
Banca: Ricardo de Oliveira Orsi
Banca: Gustavo Rocha de castro
Banca: Paulo dos Santos Roldan
Doutor
Ghafouri, Bijar. "Proteomics of the upper airways : studies on a new lipopolysaccharide-binding protein, PLUNC /." Linköping : Dept. of Molecular and Clinical Medicine, Linköping University, 2005. http://www.bibl.liu.se/liupubl/disp/disp2005/med927s.pdf.
Full textGuterres, Sheila Barreto. "Busca de biomarcadores para esquizofrenia em plaquetas utilizando eletroforese diferencial em gel bidimensional (2D-DIGE) e espectrometria de massas." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-16112011-150931/.
Full textSchizophrenia is a disabling, serious, and chronic illness, which affects about 24 million people worldwide. It is characterized by a severe disorganization of the thoughts that harms the social life of patients becoming them dependent of the family and/or government. There are effective treatments that contribute to decrease the prevalence of the disorder because they improve the life and social conditions of the patients, but they are only advantageous if the intervention is made in the early stages of the disease. It is difficult to obtain early diagnosis due to the complexity of the disease and its insidious symptoms before the beginning of the psychosis. The brain is not easily accessed in vivo and, because of this, it is very important to study the peripheral tissues like blood, which makes the use of the platelets very interesting. Furthermore, platelets and serotonergic neurons share biochemical and morphological characteristics that allows the comparison between structure and function of both. From these similarities many authors has used platelets as a neuron model to study many neurodegenerative diseases including schizophrenia. The early detection of schizophrenia is a current and suitable goal, not only to improve the early diagnosis but also to develop new treatments, differentiate the subtypes, and monitor the preventive interventions. The purpose of this project is to do a comparative screening of expressed proteins in platelets from schizophrenics and controls with the objective of finding differently expressed proteins that could be candidates to biomarkers using 2D-DIGE and mass spectrometry.
Nguyen-Lefebvre, Anh Thu. "Implication des protéines ribosomiques dans le processus de transformation induit par l’oncogène v-erbA." Thesis, Lyon 1, 2012. http://www.theses.fr/2012LYO10058/document.
Full textThe v-erbA oncogene transforms chicken erythroid progenitors by blocking their differentiation andpreventing them to exit a state of self-renewal. The transcriptome of primary avian erythroidprogenitors cells (T2EC) expressing either v-erbA or a non-transforming form of v-erbA werecompared by SAGE. Only some, but not all, mRNAs encoding ribosomal proteins were shown to beaffected. These results suggest that v-erbA could modulate the composition of ribosomes and/ormodulate the extraribosomal functions of specific ribosomal proteins. We therefore decided to analyzethe level of ribosomal proteins associated to ribosomes by 2D-DIGE performed on purified ribosomes.A statistical analysis performed on 4 independent flip-flop experiments demonstrated that the level ofRPL11 is significantly lower in T2EC expressing v-erbA as compared to the non-transforming form ofv-erbA. These data suggest the presence of ribosomes without RPL11 in T2EC expressing v-ErbA.Results obtained from immunoprecipitation experiments were strengthened this hypothesis. The set ofthese data evoke the involvement of ribosomal proteins, and specially RPL11, in the v-erbAtransformation process both at the translational level and possibly in its extra-ribosomal function.Overexpression of RPL11 in T2EC showed a decrease of cell proliferation
Chen, Zhaoyuan. "Development of Methods for the Study of Phosphoproteins." Diss., CLICK HERE for online access, 2006. http://contentdm.lib.byu.edu/ETD/image/etd1629.pdf.
Full textSayahtaheri, Sousan. "Two-Dimensional Gel Electrophoresis of in Vivo and in Vitro Synthesized Proteins, Antigenic Proteins, and Cross-Reactive Antigens in Treponema Pallidum Subsp. Pallidum Nichols Strain and Treponema Phagedenis Biotype Reiter." Thesis, University of North Texas, 1989. https://digital.library.unt.edu/ark:/67531/metadc935721/.
Full textDaveau, Romain. "One-Dimensional Electrophoresis Gel Anlalysis Tool, ODEGAT. Elaboration d’un outil bio-informatique d’aide à la mise en évidence de marqueurs à visée diagnostique et pronostique : application à la polyarthrite rhumatoïde." Rouen, 2008. http://www.theses.fr/2008ROUES033.
Full textRheumatoid arthritis (RA) still represents a serious challenge in terms of public health. Remaining the most frequent chronic inflammatory rheumatism, RA is an auto-immune disease with multiple origins. Moreover, its clinical presentation as well as its evolution are heterogeneous. RA generally consists in a bilateral and symmetrical inflammation of small and intermediate joints, responsible for severe bone destruction and leading to heavy functional consequences and disability for patients. Patient management is also highly complicated by the absence of clear criteria for diagnosis and prognosis of the disease. Yet, therapeutic solutions exist : anti-TNF-, IL-1 Ra, anti-CD20. . . These costly specific drugs are more efficient when administered early, but are often used as a second line of treatment after classical therapies have failed. 10 – 15% of RA are considered as severe and require such treatments. They thus need to be identified early to insure optimal management and prevent the occurence of irreversible lesions. However, none of the existing clinical or biological indicators commonly used allow to distinguish such severe forms of RA. This work focuses on the main questions of early diagnosis and prognosis of RA based on a cohort of patients with early inflammatory rheumatisms (VErA) and using appropriate computer languages (Perl, R). In terms of diagnosis, contribution of genetic factors (HLA-DR1, TNFRII*196R et PTPN22*1858T) turned out to be limited relatively to auto-antibodies which remain the most pertinent markers. As for prognosis, a pilot study performed on synovial biopsies demonstrated the role of tissue CD20 levels as predictive of articular lesions at 3 years in a panel of 14 patients from a linear regression model associating rheumatoid factors, anti-citrullinated protein antibodies (ACPA) and RANKL (bone remodelling marker). Confronted to the insufficiency in diagnostic and prognostic markers, search for new immunological targets as part of the ACPRA program led us to develop a new bioinformatics tool dedicated to 1-D electrophoretic gels named ODEGAT. 110 immunoblots of patient sera from the VErA cohort were tested and ODEGAT contributed to the identification of 4 original autoantibodies : anti-PGK1, -STIP1, -FUSE-BP 1 et 2. At least one of these markers was present in 40% of RA initially tested negative for ACPA. Finally, in a comparison of 10 healthy controls and 21 patients divided in 11 severe RA and 10 benign, ODEGAT revealed 5 polypeptidic bands with relative expression levels correlated to short term radiologic impact with a negative (resp. Positive) predictive value of 90% (resp. 82%). As a perspective of this PhD work and as main objective of the APOPTRA project, this last promising result will soon permit the development of a protein array which will be tested on a large number of RA, from the national ESPOIR cohort
Chen, Kan. "Applications of Mass Spectrometry to Analysis of Prodiginines, Bioactivated Methylenedianiline Intermediates, and Hypoxia Induced Changes in the Zebrafish Skeletal Muscle Proteome." ScholarWorks@UNO, 2008. http://scholarworks.uno.edu/td/899.
Full textHirschberg, Daniel. "Sample preparation and mass spectrometry in proteome studies /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-934-x/.
Full textNimako, George K. "DETERMINATION OF THE AMINO TERMINUS OF MITOCHONDRIAL GLYOXALASE II ISOZYMES USING A PROTEOMIC APPROACH." Miami University / OhioLINK, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=miami1071255221.
Full textGrinyer, Jasmine. "Proteomic analysis of the biological control fungus Trichoderma." Doctoral thesis, Australia : Macquarie University, 2007. http://hdl.handle.net/1959.14/12407.
Full text"August 2006"
Thesis (PhD)--Macquarie University, Division of Environmental & Life Sciences, Dept. of Biological Sciences & Dept. of Chemistry & Biomolecular Sciences), 2007.
Bibliography: leaves 157-183.
1. Introduction -- 1.1. Proteomics and two-dimensional electrophoresis -- 1.2. A proteomic approach to study the filamentous fungus Trichoderma -- 1.3. Aims of the thesis -- 2. Materials and methods -- 3. Results and discussion -- 3.1. Method development for the display and identification of fungal proteins by 2DE and mass spectrometry -- 3.2. Discovery of novel determinants in the biological control of phytopathogens by Trichoderma atroviride -- 3.3. Summary and concluding remarks.
Trichoderma harzianum and T. atroviride are filamentous fungi commonly found in soil. Both display biocontrol capabilities against a range of phytopathogenic fungi including Rhizoctonia solani and Botrytis cinerea which are known pests of hundreds of commercially important crops including tomatoes, potatoes, beans, cucumber, strawberries, cotton and grapes. These Trichoderma species secrete a combination of enzymes degrading cell walls and antibiotics to overgrow and kill fungal phytopathogens. They are seen as an environmentally friendly alternative to chemical fungicides currengly used on crops.
A proteomic approach was taken to separate and identify proteins from a strain of T. harzianum with well established biocontrol properties. Several methods were developed in this thesis to display the whole proteome content and several subcellular proteome fractions from T. harzianum. Proteins were separated by two-dimensional electrophoresis and identified by mass spectrometric methods. The resulting proteomic maps represent the first extensive array of cellular and sub-cellular proteomes for T. harzianum.
Cellular protein patterns of T. atroviride (T. harzianum P1) grown on media containing either glucose or R. solani cell walls were compared by differential gel electrophoresis to identify a suite of new proteins involved in the biological control response. Twenty four T. atroviride protein spots up-regulated in the presence of the R. solani cell walls were identified by mass spectrometry and N-terminal sequencing. Proteins identified from this study included previously implicated enzymes degrading cell walls and three novel proteases, vacuolar serine protease, vacuolar protease A and trypsin-like protease. The genes encoding two of these proteases, vacuolar protease A and vacuolar serine protease have been cloned by degenerate primer PCR and genomic walking PCR and sequenced. The gene sequences and protein sequences derived from these genes have been partially characterised.
Mode of access: World Wide Web.
194 leaves ill
Paspuleti, Sreelatha. "Isolation and Identification of O-linked-β-N-acetylglucosamine Modified Proteins (O-GlcNAc) in the Developing Xenopus laevis Oocyte." Scholar Commons, 2004. https://scholarcommons.usf.edu/etd/809.
Full textAbbaraju, Naga Vijayalaxmi. "Patterns of protein expression in tissues of the killifish, Fundulus heteroclitus and Fundulus grandis." ScholarWorks@UNO, 2011. http://scholarworks.uno.edu/td/113.
Full textMartins, Carlo de Oliveira. "Análise proteômica diferencial em válvula mitral na doença reumática cardíaca." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/5/5146/tde-02082013-142739/.
Full textRheumatic Heart Disease (RHD) is a serious complication of oropharingitis caused by some serotypes of Streptococcus pyogenes not properly treated in susceptible individuals. It is a public health concern, mainly for undeveloped and developing countries, such as Brazil, India, some countries in Africa, aboriginal regions in Australia, and Egypt. It is highly debilitating with a high mortality rate due to cardiac commitment. Initial myocardial lesions disappear, but valvar lesions, mainly mitral and aortic, are irreversible and progressive. Many studies have characterized cellular (T lymphocytes) and humoral responses in individuals affected by the disease. Molecular mimicry and epitope spreading are the main mechanisms thought to be involved in the pathogenesis of RHD. We evaluated, in this research, the profile of protein expression in mitral valves from individuals affected by RHD. To detect alterations specific of this disease, we compared protein expression in the group of RHD with regurgitation (RHD-RGT) and stenosis (RHD-STN) to a group of individuals with mitral valve myxomatous degeneration (MXD) and another group without valvulopathies (CTL). Alterations specifically observed in the mitral tissue of RHD-RGT and RHD-STN in advanced stages of the disease can explain the mechanism of development for these two kinds of lesions. Twenty-five spots, corresponding to 29 proteins were found to be differentially expressed in the valvulopathy groups, reflecting mainly alterations in extracellular matrix. We found important differential cleavage of vimentin, the whole protein having 54 kDa, in fragments with ~40 and ~45 kDa, increased in RHD, mainly in RHD-RGT. Collagen type-VI, with approximatelly 95 kDa, was found to have decreased expression exclusivelly in the RHD-RGT group. Increased expression of Vitronectin was detected in DMX and RHD-EST groups, compared to the CTL group, mainly in the RHD-STN. Lumican, in turn, had decreased expression in the MXD and RHD-STN groups. By using in silico methods for analysis of patterns of protein expression, we identified sets of proteins capable of discriminating mitral valve samples by disease etiology. The present study might help elucidating the mechanisms of disease development and structural alterations in the mitral tissue in response to the autoimmune lesions, as well as in the diagnosis of RHD.
Santos, Marcelo Augusto Cortina Gonçalves dos. "Detecção e rastreamento de mutações no proto-oncogene RET em pacientes com neoplasia endócrina múltipla tipo 2 por meio de eletroforese em gel sensível à conformação." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-06062007-170334/.
Full textMultiple endocrine neoplasia type 2 (MEN2) is an autosomal dominant inherited tumor syndrome caused by activating germline mutations in RET proto-oncogene (RET). Presently, the prophylactic total thyroidectomy is recommended to all RET mutations carriers. Here we tested the Conformation Sensitive Gel Electrophoresis (CSGE) as a screening method for the RET hot-spot mutations. Seven MEN2 families were studied by CSGE, as well as by Single Strand Conformational Polymorphism (SSCP) and direct sequencing analysis. Using CSGE and SSCP, we were able to detect five out of the six (83.3%) RET mutations verified by direct sequencing analysis: Cys620Arg, Cys634Arg, Cys634Tyr, Val648Ile and Met918Thr. RET polymorphisms 691 and 769 were verified by CSGE and SSCP. In our sample, data obtained using CSGE were fully concordant (100%) with SSCP findings. Thus, CSGE showed to be a sensitive, fast, low-cost, and ease procedure to detect RET mutations in codons 620, 634, 648, and 918 which are reported as the most prevalent RET variants (~95%) in large MEN2 series. As to the Val804Met mutation (prevalence in the population lower than 3%), this method still needs to be optimized. We concluded that CSGE is an effective screening method for the most frequent RET hot-spot disease-causing mutations.
Rosengren, Åsa. "Cell-protein-material Interactions on Bioceramics and Model Surfaces." Doctoral thesis, Uppsala University, Surface Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4688.
Full textThe objective of this thesis was to investigate and characterize the interaction between blood proteins and different surfaces with emphasis on protein adsorption to bioceramics and model surfaces. Special effort was made to monitor the spontaneous and selective adsorption of proteins from human plasma and to examine the orientation, conformation and functional behavior of single proteins after adsorption.
Five different ceramic biomaterials: alumina (Al2O3), zirconia (ZrO2), hydroxyapatite (Ca10(PO4)6(OH)2) and two glass-ceramics, AP40 (SiO2-CaO-Na2O-P2O5-MgO-K2O-CaF2) and RKKP (AP40 with Ta2O3-La2O3), were exposed to human plasma and their protein binding capacities and affinities for specific proteins were studied by chromatography, protein assays, two-dimensional gel electrophoresis and Western blotting. The studies showed that all materials adsorbed approximately the same high amount of plasma proteins and that they therefore should be fully covered by proteins in an in vivo setting. The adsorbed proteins were different for most materials which could explain their previously observed different levels of tissue integration in vivo.
Four of the proteins that behaved differently, ceruloplasmin, prothrombin, α2-HS-glycoprotein and α1-antichymotrypsin, were selected for characterization with atomic force microscopy and ellipsometry. The studies, which were performed on ultraflat silicon wafers (silica), showed that the proteins oriented themselves with their long axis parallel to the surface or as in case of ceruloplasmin with one of its larger sides towards the surface. All of them had globular shapes but other conformational details were not resolved. Furthermore, prothrombin (none of the others) formed multilayers at high proteins concentrations.
The functional behaviour of the adsorbed proteins, referring to their cell binding and cell spreading capacity on silica and a positive cell adhesion reference surface (Thermanox®), was affected by the underlying substrate. Ceruloplasmin, α2-HS-glycoprotein and α1-antichymotrypsin stimulated cell attachment to silica, but suppressed attachment to Thermanox®. Prothrombin stimulated cell attachment to both surfaces. The attachment was in most cases mediated both by cell membrane-receptors (integrins) and by non-specific interactions between the cell and the material.
This thesis showed that the compositional mixture, orientation, conformation and functional behavior of the adsorbed proteins are determined by the properties of the underlying surface and if these parameters are controlled very different cellular responses can be induced.
Muchindu, Munkombwe. "Electrochemical ochratoxin a immunosensors based on polyaniline nanocomposites templated with amine- and sulphate-functionalised polystyrene latex beads." Thesis, University of the Western Cape, 2010. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_3815_1306752491.
Full textPolyaniline nanocomposites doped with poly(vinylsulphonate) (PV-SO3 &minus
) and nanostructured polystyrene (PSNP) latex beads functionalized with amine (PSNP-NH2) and sulphate (PSNP-OSO3 &minus
) were prepared and characterised for use as nitrite electro-catalytic chemosensors and ochratoxin A immunosensors. The resultant polyaniline electrocatalytic chemosensors (PANI, PANI|PSNP-NH2 or PANI|PSNP-OSO3 &minus
) were characterized by cyclic voltammetry (CV), ultraviolet-visible (UV-Vis) spectroscopy and scanning electron microscopy (SEM). Brown-Anson analysis of the multi-scan rate CV responses of the various PANI films gave surface concentrations in the order of 10&minus
8 mol/cm. UV-vis spectra of the PANI films dissolved in dimethyl sulphoxide showed typical strong absorbance maxima at 480 and 740 nm associated with benzenoid p-p* transition and quinoid excitons of polyaniline, respectively. The SEM images of the PANI nanocomposite films showed cauliflower-like structures that were <
100 nm in diameter. When applied as electrochemical nitrite sensors, sensitivity values of 60, 40 and 30 &mu
A/mM with corresponding limits of detection of 7.4, 9.2 and 38.2 &mu
M NO2 &minus
, were obtained for electrodes, PANI|PSNP-NH2, PANI and PANI|PSNP-SO3 &minus
, respectively. Immobilisation of ochratoxin A antibody onto PANI|PSNP-NH2, PANI and PANI|PSNPSO3 - resulted in the fabrication of immunosensors.
Vileigas, Danielle Fernandes. "Proteoma miocárdico de ratos obesos por dieta Ocidental com disfunção cardíaca." Botucatu, 2019. http://hdl.handle.net/11449/181435.
Full textResumo: A obesidade é uma doença metabólica complexa considerada uma pandemia global e associada à alta incidência de doença cardiovascular. O excesso de tecido adiposo pode promover mal adaptação que resulta em alterações na estrutura e função do coração; no entanto, os mecanismos não estão totalmente elucidados. A proteômica pode fornecer uma compreensão mais profunda do processo fisiopatológico e contribuir para a identificação de novos potenciais alvos terapêuticos. Portanto, o objetivo deste estudo foi avaliar a expressão proteica miocárdica em ratos saudáveis e obesos por dieta Ocidental, empregando duas abordagens proteômicas, para melhor compreender a rede de mecanismos inerentes à disfunção cardíaca na obesidade. Ratos Wistar foram distribuídos em dois grupos: controle (C, n = 13; dieta controle) e obeso (Ob, n = 13; dieta Ocidental) alimentados por 41 semanas. A obesidade foi determinada pelo índice de adiposidade. A função cardíaca foi avaliada pelo ecocardiograma e análise do músculo papilar isolado. A proteômica foi baseada em eletroforese em gel bidimensional (2-DE) juntamente com espectrometria de massa (LC-MS/MS) e cromatografia-líquida em nanofluxo com espectrometria de massa em tandem (nanoLC-MS/MS) seguida de quantificação label-free. Ratos obesos apresentaram aumento do índice de adiposidade e disfunção cardíaca sistólica e diastólica comparados aos controles. Um total de 82 proteínas miocárdicas foram identificadas como diferencialmente expressas entre os grupos ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Obesity is a complex metabolic disease considered a global pandemic and associated with high incidence of cardiovascular disease. The excess of adipose tissue may promotes maladaptation that result in alterations in structure and function of the heart; however, the mechanisms are not fully elucidated. Proteomics may provide a deeper understanding into the pathophysiological process and contribute to the identification of new potential therapeutic targets. Thus, the aim of this was evaluate the myocardial protein expression in healthy and obese rats, employing two proteomic approaches to better comprehend the network of mechanisms inherent to cardiac dysfunction in obesity. Male Wistar rats were distributed into two groups: control (C, n=13; standard diet) and obese (Ob, n=13; Western diet) fed for 41 weeks. The obesity was determined by adipose index. Cardiac function was evaluated by echocardiogram and isolated papillary muscle analysis. The proteomics was based on two-dimensional gel electrophoresis (2-DE) along with mass spectrometry identification (LC-MS/MS) and nano-liquid chromatography with tandem mass spectrometry (nanoLC-MS/MS) followed by label-free quantification. Obese rats showed increased adiposity index and systolic and diastolic cardiac dysfunction. A total of 82 myocardial proteins was identified as differentially expressed between C and Ob groups using two proteomic strategies, being 43 up- and 39 down-regulated by obesity. These proteins are involved in imp... (Complete abstract click electronic access below)
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Zabel, Claus. "Veränderungen im Proteom von Maus und Mensch durch Huntington's Chorea." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2003. http://dx.doi.org/10.18452/14825.
Full textHuntington disease is an autosomal dominantly inherited disease that usually starts in midlife and inevitably leads to death. In an effort to identify proteins involved in processes upstream or downstream of the disease causing huntingtin, the proteome of a well-established mouse model was studied by large-gel 2D electrophoresis. It could be demonstrated for the first time at the protein level that two serin protease inhibitors, alpha1-antitrypsin and contraspin and the chaperone alphaB-crystallin decrease in expression over the course of disease. Importantly, the alpha1-antitrypsin decrease in the brain precedes that in liver, heart and testes in mice. Reduced expression of alpha1-antitrypsin and contraspin could be detected in the brain, liver heart and testes close to terminal disease. Decreased expression of the chaperone alphaB-crystallin was found exclusively in the brain. Reduced expression of the liver specific major urinary proteins not found in the brain, was seen in affected mice, demonstrating that the disease exerts its influence on a protein not present in the brain of transgenic mice at the protein level. When investigating three human brain regions obtained post-mortem from Huntington s disease patients, alpha1-antitrypsin expression was also altered. Maintaining alpha1-antitrypsin and alphaB-crystallin availability during the course of Huntington s disease might prevent neuronal cell death and therefore could be useful in delaying the disease progression.
Guerrero, Barrado Pedro Enrique. "Altered glycosylation in pancreatic cancer: development of new tumor markers and therapeutic strategies." Doctoral thesis, Universitat de Girona, 2020. http://hdl.handle.net/10803/671007.
Full textEl adenocarcinoma ductal pancreático o PDA, es el tipo más frecuente de cáncer de páncreas. El PDA es uno de los tumores más letales, con tasas de supervivencia extremadamente bajas, debido principalmente al diagnóstico tardío y su amplia resistencia a las terapias actuales. La expresión de antígenos carbohidratos asociados a tumores como el SLeX potencia varias características tumorales como la migración celular o metástasis, por lo cual representa una buena fuente de nuevas dianas terapéuticas y biomarcadores. En este trabajo, se ha estudiado el efecto de la inhibición de la expresión de los principales genes que codifican para las enzimas responsables de la biosíntesis de SLeX en sus etapas finales (las α2,3-sialiltransferasas). El silenciamiento de ST3GAL3 y ST3GAL4 en dos líneas celulares de PDA produjo a una reducción significativa en los niveles de SLeX. Este descenso se asoció con una disminución en la migración, invasión y adhesión celular a E-selectina, disminuyendo el potencial metastásico del PDA. Además, mediante técnicas glico-proteómicas, pudimos identificar la glicoproteína MFAP4 portadora de SLeX como potencial biomarcador de PDA, ya que esta glicoforma solo fue detectada en la cohorte de muestras de tejido de PDA