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Relevant bibliographies by topics / 2 dimensional gel electrophoresis

Academic literature on the topic '2 dimensional gel electrophoresis'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 March 2023

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Journal articles on the topic "2 dimensional gel electrophoresis"

1

Lehr, Stefan, and Reiner Westermeier. "2-dimensional gel electrophoresis reloaded." Archives of Physiology and Biochemistry 119, no. 3 (July 2013): 93. http://dx.doi.org/10.3109/13813455.2013.812122.

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Deyl, Zdeněk. "Two dimensional gel electrophoresis of proteins: methods and applications." Journal of Chromatography B: Biomedical Sciences and Applications 377 (January 1986): 477. http://dx.doi.org/10.1016/s0378-4347(00)80814-2.

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Per, S. R., J. L. Abruzzo, and R. Heimer. "Analysis of immune complexes by two-dimensional gel electrophoresis." Clinical Immunology and Immunopathology 34, no. 2 (February 1985): 165–73. http://dx.doi.org/10.1016/0090-1229(85)90021-2.

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Acevedo, Fernando, and Zere Goitom. "Two-dimensional electrophoresis: agarose gel isotachophoresis followed by sodium dodecyl sulphate—polyacrylamide electrophoresis." Journal of Chromatography A 545, no. 2 (June 1991): 343–47. http://dx.doi.org/10.1016/s0021-9673(01)88725-2.

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MARULLO, OSVALDO, ALESSIO SOGGIU, and ENRICO CAPOBIANCO. "TWO-DIMENSIONAL ELECTROPHORESIS GEL IMAGES SCAN FOR DECOMPOSITION AND DEPLETION ANALYSIS." Advances in Adaptive Data Analysis 02, no. 03 (July 2010): 359–71. http://dx.doi.org/10.1142/s1793536910000525.

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Two-dimensional Electrophoresis Gel Images Scan (2dEGIS) implements a mix of computational methods for processing two-dimensional electrophoresis gel images. For advancing the analysis in case-control sample studies, a multi-component decomposition-approximation approach is presented, based on: (1) A global scan aimed to detect discriminative patterns with just a few components; (2) A more localized image scan through aggregated components; (3) The exploration of specific regions with maximal localization power. The tool 2dEGIS represents a novel unifying instrument for the computational analysis of gel images.

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Veeser, Stefan, Michael J. Dunn, and Guang-Zhong Yang. "Multiresolution image registration for two-dimensional gel electrophoresis." PROTEOMICS 1, no. 7 (July 2001): 856–70. http://dx.doi.org/10.1002/1615-9861(200107)1:7<856::aid-prot856>3.0.co;2-r.

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Parent, Jean-Guy, Richard Hogue, and Alain Asselin. "Glycoproteins, enzymatic activities, and b proteins in intercellular fluid extracts from hypersensitive Nicotiana species infected with tobacco mosaic virus." Canadian Journal of Botany 63, no. 5 (May 1, 1985): 928–31. http://dx.doi.org/10.1139/b85-123.

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Intercellular fluid b proteins from hypersensitive Nicotiana tabacum L. cv. Xanthi-nc and N. sylvestris Speg. and Comes infected with tobacco mosaic virus were compared by two-dimensional (2-D) polyacrylamide gel electrophoresis. Except for missing bands b2, b6a, b6b, and b7b, the overall 2-D electrophoretic pattern of N. sylvestris intercellular fluid proteins was similar to the one observed with 'Xanthi-nc' tobacco. Intercellular proteins were also studied by chromatography on con-canavalin A. Glycoproteins corresponding to b6a and b7a proteins of N. tabacum and the [Formula: see text] analog of N. sylvestris were identified. These proteins are probably peroxidase isozymes, as peroxidase activities with the same electrophoretic mobility were detected after polyacrylamide gel electrophoresis. No esterase activity was associated with any b protein band in gels. Esterase activities decreased upon virus infection, but accumulation of b proteins and peroxidase activities increased.

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Hochstrasser, D., V. Augsburger, T. Pun, D. Weber, C. Pellegrini, and A. F. Muller. ""High-resolution" mini-two-dimensional gel electrophoresis automatically run and stained in less than 6 h with small, ready-to-use slab gels." Clinical Chemistry 34, no. 1 (January 1, 1988): 166–70. http://dx.doi.org/10.1093/clinchem/34.1.166.

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Abstract Although two-dimensional (2-D) gel electrophoresis is one of the most powerful techniques for analyzing protein mixtures, its application in routine clinical laboratories is currently limited, because it is time-consuming, complex, and relatively expensive. Here we describe a method for automatically running and staining "high-resolution" mini 2-D electrophoresis gels in less than 6 h, by using "ready-to-use" slab gels and a PhastSystem electrophoresis apparatus. We present 2-D gel electrophoretograms of 25 nL of plasma, as well as their automatic computer analysis. For comparison, a conventional 2-D gel electrophoresis profile of 200 nL of a plasma sample is shown. The technique is easy to perform, highly sensitive, rapid, and potentially useful in semi-routine clinical chemistry laboratories.

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Righetti, Pier Giorgio, Annalisa Castagna, Ben Herbert, and Giovanni Candiano. "How to Bring the “Unseen” Proteome to the Limelight via Electrophoretic Pre-Fractionation Techniques." Bioscience Reports 25, no. 1-2 (February 4, 2005): 3–17. http://dx.doi.org/10.1007/s10540-005-2844-2.

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The present review reports a panoply of electrophoretic methods as pre-fractionation tools in proteomic investigations in preparation for mass spectrometry or two-dimensional electrophoresis map analysis. Such electrophoretic pre-fractionation protocols include all those electrokinetic methodologies which are performed in free solution, most of them relying on isoelectric focusing steps (although some approaches based on gels and granulated media are also discussed). Devices associated with electrophoretic separations are multi-chamber apparatuses, such as the multi-compartment electrolyzers equipped with either isoelectric membranes or with isoelectric beads, Off-Gel electrophoresis in a multi-cup device and the Rotofor, an instrument also based on a multi-chamber system but exploiting the conventional technique of carrier-ampholyte-focusing. Other free-flow systems, as well as miniaturized chambers, are also described.

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Deng, Ruixue, Zhaohui Lu, Yuanjia Chen, Lu Zhou, and Xinghua Lu. "Plasma Proteomic Analysis of Pancreatic Cancer by 2-Dimensional Gel Electrophoresis." Pancreas 34, no. 3 (April 2007): 310–17. http://dx.doi.org/10.1097/mpa.0b013e31802f2483.

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Dissertations / Theses on the topic "2 dimensional gel electrophoresis"

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Yao, Mingyi. "Proteomic studies of Pseudomonas putida KT2440 in carcinogenicity screening via 2-dimensional gel electrophoresis /." Online version of thesis, 2005. https://ritdml.rit.edu/dspace/handle/1850/1118.

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Rajagopal, Meena Uma. "METHODS DEVELOPMENT AND APPLICATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS AND MASS SPECTROMETRY IN PROTEOMICS." UKnowledge, 2006. http://uknowledge.uky.edu/gradschool_diss/292.

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The development of a highly sensitive ruthenium-based fluorescent staining solution isdescribed in this dissertation. The in-house synthesized ruthenium complex (RuMS)containing both sulfonated and non-sulfonated ligand has detection limit of 1 ng ofprotein that is better than colloidal coomassie, silver and ruthenium complex containingall sulfonated ligands (RuBPS). RuMS stain has 100-fold dynamic range and does notinterfere with subsequent mass spectral identification of proteins. The capability of inhousesynthesis of the staining solution makes it a viable cost-effective alternative to theexpensive commercially available fluorescent stain, Sypro Ruby. The low detection limit,broad linear dynamic range and compatibility with mass spectrometry, make thedevelopment of this stain a worthwhile pursuit. The staining solution was utilized insubsequent applications of two-dimensional gel electrophoresis (2-DE) technology.Proteomics methodology utilizing 2-DE and mass spectrometry was applied toinvestigate the effect of malathion on the proteome of human neuroblastoma cells.Results indicated that out of 122 proteins that were identified from the neuroblastomaproteome, sixteen proteins were down-regulated while five proteins were significantlyup-regulated after treatment with malathion. Significant down-regulation of calciummodulators like calmodulin and calgizarrin and other key chaperones makes themalathion-treated cells highly prone to oxidative stress. With increased awareness inpesticide related adverse effects, identification of altered proteins in malathion-treatedhuman neuroblastoma cells is a critical finding.Proteomics is a major area of research in the identification of biomarkers for diseases. Anovel immunoprecipitation method developed in this work allowed for successfulisolation and identification of albumin-interactome in cerebrospinal fluid (CSF) that isusually under-represented in standard CSF analysis using 2-DE. A key finding is thedifferential expression of various isoforms of proteins in CSF albumin-interactome fromAlzheimer's disease (AD) subjects. The data implicate the acidic isoform ofprostaglandin D2 synthase (PGDS2) as a potential biomarker for AD. An understandingof the differential expression of these protein isoforms in AD will provide insight into theetiology of the disease and this can have far-reaching implication on drug developmentleading to the cure or even preventation of the disease.

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Anduri, Sridevi. "Differential protein expression profiles in normal and intersex male smallmouth bass determined using one- and two-dimensional gel electrophoresis a thesis presented to the faculty of the Graduate School, Tennessee Technological University /." Click to access online, 2009. http://proquest.umi.com/pqdweb?index=0&did=2000377671&SrchMode=1&sid=2&Fmt=6&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1277817194&clientId=28564.

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Trein, Cristina Rodrigues. "Eletroforese bidimensional em gel de poliocrilamida do plasma seminal equino e a sua relação com a congelabilidade do sêmen." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/39292.

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O objetivo deste estudo foi avaliar o perfil proteico do plasma seminal equino utilizando eletroforese bidimensional de gel de acrilamida (2D-PAGE) e determinar se algumas das proteínas presentes estavam relacionadas com a congelabilidade do sêmen. O plasma seminal foi coletado de dez garanhões, de alta e baixa congelabilidade de sêmen, provenientes do Haras Estatal da Baixa Saxônia, na cidade de Celle, na Alemanha e rotineiramente utilizados em programas de inseminação artificial. Vinte e cinco bandas proteicas foram encontradas nos géis bidimensionais (12%) e sete delas foram identificadas por MALDI-MS. Das 25 proteínas encontradas nas amostras de plasma seminal dos garanhões, duas bandas proteicas apresentaram densidade óptica superior (P<0,05) nas amostras de garanhões de alta congelabilidade o sêmen: as bandas 5 (80-85 kDa, pI 7,54), que foi identificada como CRISP3 e a 45 (18,2 kDa, pI 5,0-5,2) identificada como HSP-2. Contrariamente a banda 7 (75,4 kDa, pI 6,9 – 7,4), identificada como lactoferrina, a 15 (26,7 kDa, pI 5,51) identificada como calicreína, a 25 (25 kDa, pI 7,54) como CRISP3 e a 35 (13,9 kDa, pI 3,8 – 4,2) que foi identificada como HSP-1, apresentaram valores de densidade óptica superior (P<0,05) nos reprodutores de baixa congelabilidade do sêmen. As proteínas foram identificadas através de espectometria de massa MALDI-MS. As evidências encontradas neste experimento mostram que existem diferenças no perfil proteico dos reprodutores de alta e baixa congelabilidade do sêmen, sugerindo as proteínas CRISP3 e a HSP-2 como possíveis marcadores da alta congelabilidade de sêmen de garanhões.
The objective of this study was to evaluate protein profile of equine seminal plasma using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and to find if any of these proteins were related to semen freezability. Seminal plasma was collected from 10 stallions of high and low semen freezability routinely used in AI programs from the State Stud of Lower Saxony and Artificial Insemination Center. Twenty five protein spots were identified from the 2 D gel (12%), seven of which were present in all samples. Of the 25 proteins found in the research stallions, two spots showed superior relative content (P<0.05) in seminal plasma samples collected from stallions with high semen freezability: the spots 5 (80-85 kDa, pI 7.54) was identified as CRISP3 and 45 (18.2 kDa, pI 5.0-5.2) was identified as HSP-2. Conversely, the spot 7 (75.4 kDa, pI 6.9 – 7.4) was identified as lactoferrin, 15 (26.7 kDa, pI 5.51) was identified as kallikrein, 25 (25 kDa, pI 7.54) was identified as CRISP3 and 35 (13.9 kDa, pI 3.8 – 4.2) was identified as HSP-1, showed superior relative protein content (P<0.05) on seminal plasma samples from stallions with low semen freezability. The proteins were identified by MALDI-MS. There were differences in the seminal plasma protein profile between stallions with high and low semen freezability. It may be suggested that CRISP3 and HSP-2 may be considered possible seminal plasma markers of high semen freezability.

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Misztal, David Richard Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Identification of cellular changes associated with increased production of human follicle stimulating hormone in a recombinant Chinese hamster ovary cell line." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2008. http://handle.unsw.edu.au/1959.4/41326.

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A proteomics approach was used to identify proteins potentially implicated in the cellular response concurrent with elevated production levels of human follicle stimulating hormone in a recombinant Chinese hamster ovary cell line (Darren cells), using zinc and sodium butyrate in the production media to increase expression. To this end, 2-dimensional gel electrophoresis (2-DGE) was utilized. Firstly, several aspects of 2-DGE were developed for this investigation. Gel drying conditions were optimized, and a glycine-free blotting method is described which achieved greater efficiency in rapid transfer of proteins than those previously described. Next, hFSH expression was characterized in Darren cells. An ELISA developed for this investigation examined intracellular (expression) and extracellular (secretion) of hFSH during increased expression. These results show a disproportionate increase in intracellular hFSH (188%) expression above extracellular hFSH (41%).

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Hadjem, Ammar Saïd. "Traitement d'image du gel d'électrophorèse bidimensionnelle." Nancy 1, 1988. http://www.theses.fr/1988NAN10143.

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L'objectif de ce travail est le traitement d'images du gel d'électrophorès bidimensionnelle (détection des taches de protéines, détermination automatique du nombre de taches de protéines sur le gel, la surface de chaque tache, leur position ainsi que leur densité optique ou en d'autres termes leur intensité lumineuse respective) en utilisant l'interface de numérisation associé à une caméra vidéo et à un micro-calculateur. Le travail a consisté, d'une part à mettre en œuvre une façon pratique cette nouvelle méthode d'investigation et d'autre part, à créer le logiciel assurant un fonctionnement jugé optimal pour ce type de fonctions (traitements d'images vidéo). L'autre partie de mon sujet est le travail expérimental en mettant au point les supports logiciels et techniques de la manipulation. En préliminaire est exposée une méthode originale de stockage des données numériques relatives à la partie comprise dans la fenêtre de mémorisation à position programmable ; la procédure de transfert de ces données dans le micro-calculateur en vue de leur traitement informatique est également présentée

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Coaker, Gitta Laurel. "Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis." The Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1069188955.

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Apraiz, Larrucea Itxaso. "Development and application of a proteomic approach to the assessment of pollution in the marine environment." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-26150.

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Today, assessment of the health of coastal waters is recognized as being important for both the conservation of nature and well-being of humans. Anthropogenic pollution has been the focus of extensive research for some time and a variety of programs for the monitoring and assessment of environmental pollution have been developed. Determination of the levels of pollution in sensitive ‘sentinels’ such as mussels, allows monitoring of these levels in a given area over a prolonged period of time. Furthermore, the biological effects of pollution are reflected in a series of biomarkers, none of which provides a general picture of the sentinel’s state of health and all of which are individually specific for certain pollutants and influenced by both biotic and abiotic factors. In an attempt to improve biomonitoring of marine pollution, we have developed two proteomic approaches here. In the first portion of the thesis, a proteomic analysis was performed on peroxisomes isolated from mussels exposed either to one of three model anthropogenic pollutants, or two different types of crude oil, or from mussels exposed to the Prestige oil spill. Application of two-dimensional electrophoresis (2-DE) provided protein expression signatures (PES) for exposure to these different pollutants.Furthermore, several individual protein components of these PES could be putatively identified. In the second portion of this work, such analysis of subproteomes was developed further in order to improve the applicability of this approach to biomonitoring. A simple fractionation procedure in combination with liquid chromatography and 2-DE provided samples from mussels residing in different regions of a pollution gradient around the harbor of Gothenburg, as well as from mussels exposed to two types of fuel oil similar to that of the Prestige that were suitable for environmental proteomics. In addition, we constructed a model for this approach that can be cross-validated in the future and applied to assess sources of fuel oil pollution in connection with biomonitoring programs.

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Qiu, Linghua. "Differentially Expressed Proteins in the Pancreas of Diabetic Mice." Ohio University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1125866599.

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Amelina, Hanna. "Proteomics in biomarker research : Insights into the effects of aging and environment on biological systems." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-56483.

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Proteomics is the global analysis of proteins that covers a broad range of technologies aimed at determining the identity and quantity of proteins expressed in the cell, their three-dimensional structure and interaction partners. In contrast to genome, proteome reflects more accurately on the dynamic state of the cell, tissue, or an organism. Therefore much is expected from proteomics to yield better disease markers for early diagnosis and therapy monitoring, as well as biomarkers that would indicate environmental exposure or provide prediction of biological age. In this thesis, I have developed and applied robust and sensitive subproteomic approaches to study the effect of aging as well as and environmental pollution using different animal models. In the first part, a high-throughput proteomic method based on liquid chromatography coupled to 2-dimensional gel electrophoresis (LC/2-DE) was developed. The usefulness of this method has been demonstrated by applying it to the assessment of marine pollution in a field experiment. Next, I have utilized this subproteomic approach to study the effect of aging in mouse kidney of both genders. As a result, a protein expression signature of aging kidney was obtained, revealing gender-dependent alterations in proteome profiles of aging mouse kidney. In order to further reduce the dynamic range of protein expression and increase the sensitivity of proteomic analysis, I have applied a shotgun mass spectrometry-based proteomic approach using isobaric tags for relative and absolute quantification (iTRAQ) coupled to liquid chromatography and tandem mass spectrometry (LC-MS/MS) to study age-related differences in peroxisome-enriched fractions from mouse liver. Only eight proteins showed statistically significant difference in expression (p<0.05) with moderate folds. This study indicates that age-depended changes in the liver proteome are minimal, suggesting that its proteome is efficiently maintained until certain age. Finally, in the context of aging studies and the role of peroxisomes in aging, I have tested the utility of cell-penetrating peptides (CPPs) as agents for protein delivery into acatalasemic peroxisomes using yeast as a model. The results obtained suggest that CPPs may be suitable for the delivery of antioxidants to peroxisomes and in future could provide a tool for the protein therapy of age-related diseases.
At the time of the doctoral defense, the following publications were unpublished and had a status as follows: Paper 3: Submitted, Paper 4: Submitted.

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Books on the topic "2 dimensional gel electrophoresis"

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Two-dimensional electrophoresis protocols. New York: Humana, 2009.

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Dunbar, Bonnie S. Two-dimensional electrophoresis, and immunological techniques. New York: Plenum Press, 1987.

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Two-dimensional electrophoresis, and immunological techniques. New York: Plenum Press, 1987.

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Margit, Burmeister, and Ulanovsky Levy, eds. Pulsed-field gel electrophoresis. Totowa, N.J: Humana Press, 1992.

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Cramer, Rainer, and Reiner Westermeier, eds. Difference Gel Electrophoresis (DIGE). Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-573-2.

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International, Two-Dimensional Electrophoresis Conference (1988 Vienna Austria). Two-dimensional electrophoresis: Proceedings of the International Two-Dimensional Electrophoresis Conference, Vienna, November 1988. Weinheim, F.R.G: VCH, 1989.

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Rabilloud, Thierry, ed. Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57105-3.

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Anderson, Leigh. Two-dimensional electrophoresis: Operation of the ISO-DALT system. Washington, D.C: Large Scale Biology Press, 1988.

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Anton, Posch, ed. 2D PAGE: Sample preparation and fractionation. Totowa, NJ: Humana Press, 2008.

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Rust, Nigel Anthony. Analysis of the human MCH class II antigens by two dimensional gel electrophoresis. Oxford: Oxford Polytechnic, 1986.

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Book chapters on the topic "2 dimensional gel electrophoresis"

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Zhang, Daohai, and Evelyn Siew-Chuan Koay. "Analysis of Laser Capture Microdissected Cells by 2-Dimensional Gel Electrophoresis." In Methods in Molecular Biology™, 77–91. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-117-8_5.

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Mettrick, Karla A., Georgia M. Weaver, and Ian Grainge. "Neutral–Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures." In Methods in Molecular Biology, 61–72. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0323-9_5.

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Turiault, Marc, Caroline Cohen, Guy Griebel, David E. Nichols, Britta Hahn, Gary Remington, Ronald F. Mucha, et al. "Two-Dimensional Gel Electrophoresis." In Encyclopedia of Psychopharmacology, 1351. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_1547.

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Kłodzińska, Ewa, and Bogusław Buszewski. "Two-dimensional Gel Electrophoresis (2DE)." In Springer Series in Chemical Physics, 133–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-35043-6_8.

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Robinson, Aisling A., Ciara A. McManus, and Michael J. Dunn. "Two-Dimensional Polyacrylamide Gel Electrophoresis." In Sample Preparation in Biological Mass Spectrometry, 217–42. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0828-0_13.

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Bergh, Gert Van den. "Two-Dimensional Difference Gel Electrophoresis." In Methods in Molecular Biology, 439–54. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-281-6_29.

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Blundon, Malachi, Vinitha Ganesan, Brendan Redler, Phu T. Van, and Jonathan S. Minden. "Two-Dimensional Difference Gel Electrophoresis." In Methods in Molecular Biology, 229–47. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8793-1_20.

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Minden, Jonathan S. "Two-Dimensional Difference Gel Electrophoresis." In Methods in Molecular Biology, 287–304. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-821-4_24.

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Römling, Ute, Karen Schmidt, and Burkhard Tümmler. "One-dimensional Pulsed-field Gel Electrophoresis." In Bacterial Genomes, 312–25. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_25.

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Römling, Ute, Karen Schmidt, and Burkhard Tümmler. "Two-dimensional Pulsed-field Gel Electrophoresis." In Bacterial Genomes, 326–36. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_26.

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Conference papers on the topic "2 dimensional gel electrophoresis"

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Rogers, M., J. Graham, and R. P. Tong. "2 Dimensional Electrophoresis Gel Registration Using Point Matching and Local Image-Based Refinement." In British Machine Vision Conference 2004. British Machine Vision Association, 2004. http://dx.doi.org/10.5244/c.18.59.

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"MATCHING TWO-DIMENSIONAL GEL ELECTROPHORESIS’ SPOTS." In International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2012. http://dx.doi.org/10.5220/0003702401110117.

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Abshire, T., L. Fink, J. Christian, J. O'Connell, and W. Hathaway. "THE DYSFIBRINOGEN OF CHILDHOOD NEPHROSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643334.

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An abnormal fibrinogen (Fib) related to increased sialic acid (SA) has been described in adults with liver disease. This dysfibrinogen (Dysfib) seems most like fetal Fib. A review of 11 patients with nephrosis revealed an unexplained prolonged thrombin time (TT) and otherwise normal coagulation studies. Based on these observations, we sought to answer whether the prolonged TT defined a Dysfib and if this abnormal Fib was similar to fetal Fib. Pooled adult, fetal plasma, and the plasma of 3 patients with nephrosis were studied with TT and reptilase times (RT). Fib was measured by functional (Fib-act) and immunologic (Fib-ag) assays. An enzyme linked immunosorbent assay (ELISA) was established using antifibrinogen as the first antibody and either peroxidase conjugated Fib or a lectin (Limulus Polyphemus) specific for SA as the second antibody. The optical density was recorded per μgm Fib for both conjugated antifibrinogen or lectin and the ratio compared in order to estimate SA reactivity. Patient 3 was also studied by: 1) crossed immunoelectrophoresis (CIE) employing lectin in the first dimension and 2) polyacrylamide gel electrophoresis (PAGE) with transfer to nitrocellulose paper using Western Blot technique.Results of the CIE showed patient 3 and fetal plasma were similar in electrophoretic pattern and different from adult plasma. The PAGE with Western Blot revealed a similar pattern of Fib for patient 3, fetal and adult plasma. We conclude that the prolonged TT and RT, the greater amount of Fib-ag when compared to Fib-act in patients 1-3 and fetal plasma and the absence of evidence for Fib degradation products, support the diagnosis of Dysfib. The similarity of the CIE for patient 3 and fetal plasma and the difference between ELISA lectin/Fib ratio of patients 1-3 and fetal compared with adult plasma suggest that the Dysfib of nephrosis may be similar to fetal Fib.

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Xin, Hua-Mei, Yuemin Zhu, Lin Xue, Robert Goutte, and Long-Fei Wu. "New Registration Method for Two-Dimensional Gel Electrophoresis Images." In 2009 2nd International Conference on Biomedical Engineering and Informatics. IEEE, 2009. http://dx.doi.org/10.1109/bmei.2009.5305071.

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Xin, Huamei, and Fengping Zhao. "Effective denoising methods for two-dimensional gel electrophoresis images." In 2011 4th International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2011. http://dx.doi.org/10.1109/bmei.2011.6098614.

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Savelonas, Michalis, Dimitris Maroulis, and Eleftheria Mylona. "Segmentation of two-dimensional gel electrophoresis images containing overlapping spots." In 2009 9th International Conference on Information Technology and Applications in Biomedicine (ITAB 2009). IEEE, 2009. http://dx.doi.org/10.1109/itab.2009.5394327.

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Ahmad, Norhaiza, J. Zhang, P. J. Brown, D. C. James, J. R. Birch, A. J. Racher, C. M. Smales, Kamel Ariffin Mohd Atan, and Isthrinayagy S. Krishnarajah. "Proteomic Profiling Of Two-Dimensional Gel Electrophoresis Protein Expression Data." In INTERNATIONAL CONFERENCE ON MATHEMATICAL BIOLOGY 2007: ICMB07. AIP, 2008. http://dx.doi.org/10.1063/1.2883868.

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Matuzevicius, D., and D. Navakauskas. "Feature selection for segmentation of 2-D electrophoresis gel images." In 2008 International Biennial Baltic Electronics Conference (BEC2008). IEEE, 2008. http://dx.doi.org/10.1109/bec.2008.4657550.

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Ahmed, Aya Saleh, Wessam H. El-Behaidy, and Aliaa A. A. Youssif. "Automatic Enhancement of Two-Dimensional Gel electrophoresis images using Denoising Autoencoder." In 2019 14th International Conference on Computer Engineering and Systems (ICCES). IEEE, 2019. http://dx.doi.org/10.1109/icces48960.2019.9068175.

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Tung-Shou Chen, Tian-Shing Wang, Wao-Chuan Hsiao, and Chun-Wei Tsai. "An Integrated Web-Based System for Two-Dimensional Electrophoresis Gel Images." In 8th International Conference on Advanced Communication Technology. IEEE, 2006. http://dx.doi.org/10.1109/icact.2006.206002.

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Reports on the topic "2 dimensional gel electrophoresis"

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Dunn, Bruce E., Martin J. Blaser, and Edward L. Snyder. Two-Dimensional Gel Electrophoresis and Immunoblotting of Campylobacter Outer Membrane Proteins. Fort Belvoir, VA: Defense Technical Information Center, April 1987. http://dx.doi.org/10.21236/ada265461.

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Xu, Aoshuang. Development in electrophoresis: instrumentation for two-dimensional gel electrophoresis of protein separation and application of capillary electrophoresis in micro-bioanalysis. Office of Scientific and Technical Information (OSTI), January 2008. http://dx.doi.org/10.2172/1342558.

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Ginzberg, Idit, and Walter De Jong. Molecular genetic and anatomical characterization of potato tuber skin appearance. United States Department of Agriculture, September 2008. http://dx.doi.org/10.32747/2008.7587733.bard.

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Potato (Solanum tuberosum L.) skin is composed of suberized phellem cells, the outer component of the tuber periderm. The focus of the proposed research was to apply genomic approaches to identify genes that control tuber skin appearance - smooth and shiny skin is highly preferred by the customers while russeted/netted skin potatoes are rejected. The breeding program (at Cornell University) seeks to develop smooth-skin varieties but has encountered frequent difficulties as inheritance of russeting involves complementary action by independently segregating genes, where a dominant allele at each locus is required for any degree of skin russeting. On the other hand, smooth-skin varieties frequently develop unsightly russeting in response to stress conditions, mainly high soil temperatures. Breeding programs in Israel aimed towards the improvement of heat tolerant varieties include skin quality as one of the desired characteristics. At the initiation of the present project it was unclear whether heat induced russeting and genetically inherited russeting share the same genes and biosynthesis pathways. Nevertheless, it has been suggested that russeting might result from increased periderm thickness, from strong cohesion between peridermal cells that prevents the outer layers from sloughing off, or from altered suberization processes in the skin. Hence, the original objectives were to conduct anatomical study of russet skin development, to isolate skin and russeting specific genes, to map the loci that determine the russet trait, and to compare with map locations the candidate russet specific genes, as well as to identify marker alleles that associated with russet loci. Anatomical studies suggested that russet may evolve from cracking at the outer layers of the skin, probably when skin development doesn’t meet the tuber expansion rate. Twodimensional gel electrophoresis and transcript profiling (cDNA chip, potato functional genomic project) indicated that in comparison to the parenchyma tissue, the skin is enriched with proteins/genes that are involved in the plant's responses to biotic and abiotic stresses and further expand the concept of the skin as a protective tissue containing an array of plantdefense components. The proteomes of skin from heat stressed tubers and native skin didn’t differ significantly, while transcript profiling indicated heat-related increase in three major functional groups: transcription factors, stress response and protein degradation. Exceptional was ACC synthase isogene with 4.6 fold increased level in the heat stressed skin. Russeting was mapped to two loci: rusB on chromosome 4 and rusC on chromosome 11; both required for russeting. No evidence was found for a third locus rusA that was previously proposed to be required for russeting. In an effort to find a link between the russeting character and the heat-induced russeting an attempt was made to map five genes that were found in the microarray experiment to be highly induced in the skin under heat stress in the segregating russet population. Only one gene was polymorphic; however it was localized to chromosome 2, so cannot correspond to rusB or rusC. Evaluation of AFLP markers tightly linked to rusB and rusC showed that these specific alleles are not associated with russeting in unrelated germplasm, and thus are not useful for MAS per se. To develop markers useful in applied breeding, it will be necessary to screen alleles of additional tightly linked loci, as well as to identify additional russet (heat-induced and/or native) related genes.

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Eyal, Yoram, and Sheila McCormick. Molecular Mechanisms of Pollen-Pistil Interactions in Interspecific Crossing Barriers in the Tomato Family. United States Department of Agriculture, May 2000. http://dx.doi.org/10.32747/2000.7573076.bard.

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During the evolutionary process of speciation in plants, naturally occurring barriers to reproduction have developed that affect the transfer of genes within and between related species. These barriers can occur at several different levels beginning with pollination-barriers and ending with hybrid-breakdown. The interaction between pollen and pistils presents one of the major barriers to intra- and inter-specific crosses and is the focus of this research project. Our long-term goal in this research proposal was defined to resolve questions on recognition and communication during pollen-pistil interactions in the extended tomato family. In this context, this work was initiated and planned to study the potential involvement of tomato pollen-specific receptor-like kinases (RLK's) in the interaction between pollen and pistils. By special permission from BARD the objectives of this research were extended to include studies on pollen-pistil interactions and pollination barriers in horticultural crops with an emphasis on citrus. Functional characterization of 2 pollen-specific RLK's from tomato was carried out. The data shows that both encode functional kinases that were active as recombinant proteins. One of the kinases was shown to accumulate mainly after pollen germination and to be phosphorylated in-vitro in pollen membranes as well as in-vivo. The presence of style extract resulted in dephosphorylation of the RLK, although no species specificity was observed. This data implies a role for at least one RLK in pollination events following pollen germination. However, a transgenic plant analysis of the RLK's comprising overexpression, dominant-negative and anti-sense constructs failed to provide answers on their role in pollination. While genetic effects on some of the plants were observed in both the Israeli and American labs, no clear functional answers were obtained. An alternative approach to addressing function was pursued by screening for an artificial ligand for the receptor domain using a peptide phage display library. An enriched peptide sequence was obtained and will be used to design a peptide-ligand to be tested for its effect o pollen germination and tube growth. Self-incompatibility (SI) in citrus was studied on 3 varieties of pummelo. SI was observed using fluorescence microscopy in each of the 3 varieties and compatibility relations between varieties was determined. An initial screen for an S-RNase SI mechanism yielded only a cDNA homologous to the group of S-like RNases, suggesting that SI results from an as yet unknown mechanism. 2D gel electrophoresis was applied to compare pollen and style profiles of different compatibility groups. A "polymorphic" protein band from style extracts was observed, isolated and micro-sequenced. Degenerate primers designed based on the peptide sequence date will be used to isolate the relevant genes i order to study their potential involvement in SI. A study on SI in the apple cultivar Top red was initiated. SI was found, as previously shown, to be complete thus requiring a compatible pollinator variety. A new S-RNase allele was discovered fro Top red styles and was found to be highly homologous to pear S-RNases, suggesting that evolution of these genes pre-dated speciation into apples and pears but not to other Rosaceae species. The new allele provides molecular-genetic tools to determine potential pollinators for the variety Top red as well as a tool to break-down SI in this important variety.

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