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1
Duran, Hasan Engin <1982>. "Short-run dynamics of income disparities and cycle synchronization across regions." Doctoral thesis, Università Ca' Foscari Venezia, 2011. http://hdl.handle.net/10579/1133.
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Since the 1990s, the issue of regional income convergence and its long term tendencies has been thoroughly and heatedly discussed. Far less attention, however, has been devoted to the short-run dynamics of regional convergence. The present thesis is
devoted to explicitly studying the short-run dynamics of regional income disparities; in
particular to the interconnections between regional income inequalities and the
aggregate business cycle as well as to the interactions among disaggregate and
aggregate economic fluctuations in the US. In the first chapter, we characterize the
short-run behavior of income inequalities across states and investigate the mechanisms
behind such behavior. In Chapter 2, we investigate whether and why some economies
might be systematically ahead of others along the swings of the business cycle. Chapter 3 aims at evaluating the distortion introduced in the cross-sectional analysis of
economic convergence when the period under study contains incomplete business cycles.Dal 1990, il tema della convergenza del reddito regionale e le sue tendenze di lungo termine è stato notevolmente approfondito e discusso. Minore attenzione, tuttavia, è stata dedicata alla dinamiche di breve periodo delle convergenze regionali. La presente tesi analizza esplicitamente le dinamiche delle disparità regionali di reddito di breve periodo; in particolare, le interconnessioni tra le disuguaglianze di reddito regionale e il ciclo economico aggregato, nonché le interazioni tra le fluttuazioni economiche negli Stati Uniti sia a livello disaggregato e aggregato. Nel primo capitolo, si caratterizza il comportamento delle disparità di reddito nel breve periodo degli stati e si studiano i meccanismi dietro tale comportamento. Nel capitolo 2, vengono indagate le ragioni per cui alcune economie sistematicamente guidano le oscillazioni dei cicli economoci ed altre le seguono con dei ritardi. Il capitolo 3 è dedicato esplicitamente alle distorsioni che possono sorgere nell’analisi cross-section della convergenza quando il periodo di analisi include cicli aggregati incompleti.
2
Pampolini, Katia <1993>. "L’evoluzione della gestione informativa aziendale: benefici, criticità e supporto alle organizzazioni." Master's Degree Thesis, Università Ca' Foscari Venezia, 2017. http://hdl.handle.net/10579/11803.
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L’informazione all’interno delle aziende è un elemento fondamentale, la cui circolazione, comunicazione ed elaborazione coadiuva i membri delle organizzazioni a conoscere le proprie caratteristiche e potenzialità e prevedere future conseguenze organizzative.
All’interno di questa tesi vengono ripercorse le tappe che hanno cambiato e plasmato il modo di gestire l’informazione, mano a mano che le organizzazioni si sono sviluppate nel tempo.
Si ripercorre quindi l’evoluzione dei sistemi informativi e il modo di gestire l’informazione nelle aziende a seconda delle loro necessità. Una parte considerevole dell’elaborato si sofferma sul concetto di integrazione dei sistemi informativi, ovvero sui più avanzati sistemi informativi automatizzati: i sistemi di Enterprise Resouce Planning (ERP) indicandone peculiarità, benefici, limiti e caratteristiche.
L’elaborato pone poi l’attenzione sulle tecnologie dell’informazione maggiormente avanzate, quelle che permettono alle organizzazioni di gestire il complesso informativo aziendale nel modo migliore possibile per informazioni di tipo differenti, e a seconda di diverse esigenze, anche dal punto di vista economico-organizzativo. Infine viene descritto un esempio pratico di sistema informativo, riscontrato durante l’esperienza di stage universitario, relativo alle società tra professionisti, il quale permette a queste la gestione contabile contemporanea sia secondo il principio di cassa che secondo il principio di competenza.
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Santin, Nicolo' <1992>. "La gamification e l'advergaming: il caso Ofree e la soluzione per far donare la gente alle organizzazioni non profit senza mettere mano al portafoglio." Master's Degree Thesis, Università Ca' Foscari Venezia, 2017. http://hdl.handle.net/10579/11603.
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La tesi è incentrata sulla gamification, presentando i vari campi di applicazione di questo fenomeno, e successivamente si focalizza in particolare sugli advergame. Segue poi una ricerca, condotta tramite interviste e questionari, che si ricollega ai temi trattati nei precedenti capitoli. L'ultima parte riguarda il caso di studio Ofree. Si tratta di una piattaforma in fase embrionale che si propone di far donare dei soldi alle persone in maniera completamente gratuita.
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SPREAFICO, MARTA. "Institutions and Growth: The Experience of the Former Soviet Union Economies." Doctoral thesis, Università Cattolica del Sacro Cuore, 2011. http://hdl.handle.net/10280/1113.
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Organizzata in tre saggi, questa tesi si pone l’obiettivo di consentire una migliore comprensione del legame tra crescita e istituzioni, e dei meccanismi attraverso cui gli assetti istituzionali possono condizionare i sentieri economici.
Riconoscendo, sulla base di considerazioni storiche, il potere esemplificativo delle ex Repubbliche Socialiste Sovietiche e della loro comune esperienza passata, questo lavoro fornisce, da un lato, una struttura empirica di riferimento per esaminare l’impatto sulla performance economica di un insieme di istituzioni, concretamente legate al funzionamento dell’attività economica; dall’altro, approfondisce lo studio degli effetti e delle determinanti delle azioni di policy.
Il primo saggio offre una disamina della letteratura riguardante il legame crescita e istituzioni, fornendo un quadro esaustivo degli sviluppi teorici ed empirici, e illustra diversi aspetti che possono essere concepiti come obiettivi per la ricerca futura; il secondo, attraverso la costruzione di un modello statico e di un modello dinamico, quantifica l’impatto delle istituzioni economiche sui sentieri di crescita di questi paesi, impiegando e analizzando numerose tecniche di stima; il terzo saggio formula diverse specificazioni e affronta il tema rilevante del ruolo degli interventi di policy sullo sviluppo economico e dell’effetto delle istituzioni politiche su comportamenti e decisioni del governo.Organized in three essays, this thesis aims at achieving a better understanding of the link between growth and institutions, and of the mechanisms through which the institutional arrangements affect the economic paths. Exploiting the past common experience of the Former Soviet Union economies, this work provides an empirical framework to examine the impact on the economic performance of a set of institutions concretely related to the “functioning” of the economic activity and offers a first attempt to include in this research program the study of the consequences of the government actions. The first essay offers a thorough review of the literature researching on the link between economic growth and institutions, and elucidates several issues that deserve further attention; the second develops a static and a dynamic approach to assess, using multiple estimation techniques, the impact of a set of economic institutions on the growth paths of these countries; the third essay, through several formal specifications, deals with the relevant issue of the role of policy measures and of the effect of the political institutions on the governments behaviour.
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PISANI, FABIO. "Three essays on credit management." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2009. http://hdl.handle.net/2108/1123.
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Nel primo articolo si dimostra che, con contratti standard di debito, ammontare del prestito e profitti del debitore non variano sia quando si tratta di un prestito individuale o di un prestito di gruppo con penalità congiunta, poiché l'effetto positivo del secondo schema sul rischio della banca (e il tasso debitore) è compensato dalla penale congiunta sui debitori.
E’ stato anche dimostrato che il prestito partecipato (debito più utili) che riduce i tassi di interesse (rispetto ai contratti di debito standard) genera maggiore richiesta di debito e output, ma inferiori profitti per il debitore. Tali contratti, tuttavia, non può essere usati in presenza informazioni nascoste ex-post, a meno che la verifica ex post da parte del creditore è possibile ed economicamente conveniente.
Tuttavia, si dimostra che (un prestito standard o partecipato) il prestito di gruppo ha un vantaggio comparato nel risolvere il problema dell’eterogeneità dei debitori, in quanto, con questi è possibile un menu di contratti che consente di discriminare tra gruppi eterogenei. Infine si dimostra che, sotto certe alcune condizione parametriche, questi contratti garantiscono profitti più elevati per i debitori meno rischiosi rispetto ad un contratto standard di debito individuale.
Nel secondo articolo si analizza l'effort di equilibrio dei debitori e il costo dei prestiti di microcredito in presenza di moral hazard, correlazione dei progetti e di sovvenzioni nel caso di prestiti di gruppo. I risultati dimostrano che nel caso di effort endogeno, la correlazione dei progetti ha effetti significativi sull’effort dei debitori solo quando ci sono shock asimmetrici (positivi o negativi). Questi risultati indicano che il ben noto effetto negativo sull’effort nel caso di prestiti di gruppo con penalità congiunta (simmetrico) nel caso di correlazione dei progetti scompare una volta che l’effort è considerato endogeno. Si analizzano inoltre gli effetti delle sovvenzioni ai prestiti (e correlazione asimmetrica) sulla relativa convenienza (in termini di effort del debitore) nel caso di i) prestito di gruppo e prestito individuali con nozionale di garanzia, ii) tra tre diverse strutture di mercato della microfinanza l'industria.
Nella terzo articolo si introducono le opzioni reali per individuare la struttura ottima del capitale in presenza di incertezza e distribuzione asimmetrica dei benefici e dei costi tra il debitore e il creditore. Il lavoro analizza sia il contesto uniperiodale sia quello multiperiodale. Il livello ottimale del debito consente di ottenere un equilibrio Pareto efficiente.
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坂, 英雄, 篤. 渡辺, 好規 長谷川, 秀造 酒井, 辰雄 大宜見, 雅史 山本, 博史 天野, et al. "P-179 癌性胸膜炎症例の予後 : 胸水と血清のパラメータを中心として." 日本肺癌学会, 1993. http://hdl.handle.net/2237/11036.
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鈴木, 裕太郎, 秀樹 村上, 晃司 河口, 哲郎 谷口, 好規 長谷川, 薫. 下方, and 好孝 関戸. "P-313 悪性胸膜中皮腫におけるPI3K/AKT経路,PTEN遺伝子変異の検討." 日本肺癌学会, 2007. http://hdl.handle.net/2237/11038.
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CARAMANICO, LEILA. "STUDY OF GRAPEVINE ROOTSTOCK RESPONSE TO WATER STRESS." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/707586.
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More than 80% of vineyards around the world use grafted plants: a scion of Vitis vinifera grafted onto a rootstock of single or interspecific hybrids of American Vitis species, resistant or partially resistant to Phylloxera (Daktulosphaira vitifoliae (Fitch, 1856)). The genetic variability of grapevine rootstocks plays a fundamental role in their adaptation to the environment (Serra et al., 2013). In the climate change scenario, predicting an increase of aridity in the near future (Dai, 2013), the more frequent and severe drought events may represent the major constrain for the future of viticulture (IPCC, 2018; Schultz, 2000). Therefore, the selection of new rootstocks able to cope with unfavourable environmental condition is a key asset, as well as a strategy to improve crop yield/vegetative growth balance on scion behaviour (Corso and Bonghi, 2014).
So far, the influence of rootstock on scion physiological performance during water stress has always aroused great interest. On the contrary, the scion impact on rootstock response is still less debated. Therefore, the effect of grafting on rootstock behaviour have been investigated. Phenotypical and large-scale whole transcriptome analyses on two genotypes, a drought-susceptible (101-14) and a drought-tolerant (1103 P), own-rooted and grafted with Cabernet Sauvignon, subjected to a gradual water shortage in semi-controlled environmental conditions have been performed. The ungrafted condition affected photosynthesis and transpiration, meaning the decisive role of scion in modulation of gas exchanges and in general in plant adaptation. Molecular evidence highlighted that the scion delays the stimulus perception and rootstock reactivity to drought.
Since 1985, the DiSAA research group operating at the University of Milan is carrying on a rootstock crossbreeding program which has led to the release of four genotypes: M1, M2, M3 and M4. They show from moderate to high tolerance to drought (M4 > M1 = M3 > M2). In order to characterize their performance during water stress, their physiological (gas exchanges and stem water potential) and transcriptome response (genes involved in ABA-synthesis and ABA-mediated responses to drought) under well-watered and water stress conditions were examined. The behaviour of M-rootstocks (M1, M2 and M3) was compared with that of other commercial genotypes largely used in viticulture, either tolerant (140 Ru, 41 B, 110 R, 1103 P), less tolerant (SO 4, K 5BB) and susceptible (420 A and Schwarzman). Discriminant analysis (DA) showed that when water availability starts to decrease, rootstocks firstly perceives the stress activating a transcriptome response, consequently physiological changes have been observed. It also demonstrated that the three M-rootstocks were clearly discriminated: M4 was grouped with the most tolerant genotypes while M3 with the less tolerant or susceptible ones from a physiological standpoint, confirming their different attitude to tolerate water stress.
M4 has proven to be a promising rootstock due to its ability to adapt to drought conditions. Considering the constant great demand for vine planting materials, the obtainment of genetically homogeneous populations (i.e. clones) from elite individuals through micropropagation represents a rapid alternative to conventional multiplication. For this reason, an efficient high-throughput protocol for M4 in vitro propagation was set up. Its attitude to shooting, root development and callus proliferation was compared to that of other rootstocks largely used in viticulture (K5BB, 1103P, 101-14 and 3309C). Moreover, pro-embryogenic and embryogenic callus from bud explants were also produced, representing a cellular material manipulable with the genetic engineering techniques.
In water scarcity condition, among the mechanisms activated by M4, the great ability to scavenge ROS, related to the increased accumulation of stilbenes and flavonoids, may be such as to give it tolerance to the stress. In particular, the higher levels of trans-resveratrol were correlated with the up-regulation of some stilbene synthase genes, mainly VvSTS16, VvSTS18, VvSTS27 and VvSTS29. The over expression of these genes was linked to a structural variation in their promoter region. To confirm that VvSTSs genes may be considered putative factors of M4 better adaptation to water stress, a genome editing protocol based on the CRISPR/Cas9 system, aimed at knock-out the genes, was performed. For testing the gRNAs functionality, a transient assay on in vitro micropropagated plantlets of M4 and 101-14 was performed. The positive results obtained by this experiment will lead to the transformation of somatic embryos and regeneration of whole-edited plants using the vectors developed.
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藤原, 豊., 征史 近藤, 俊彦 横山, 範恭 宇佐見, 香平 横井, 好孝 関戸, 和良 今泉, 裕昭 久米, and 好規 長谷川. "P-1 肺癌手術症例における血清中DNAにおけるEGFR遺伝子の変異検索." 日本肺癌学会, 2007. http://hdl.handle.net/2237/11033.
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DELPIAZZO, ELISA. "La partecipazione del Mozambico al SADC. Un processo di liberalizzazione attraverso diversi modelli e diverse chiusure." Doctoral thesis, Università Cattolica del Sacro Cuore, 2011. http://hdl.handle.net/10280/1109.
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La scelta del modeller riguardo alla chiusura del modello CGE influenza i suoi risultati finali e le sue prescrizioni di policy. In questa tesi, lo scopo è l’analisi e l’identificazione del problema, sia attraverso una discussione teorica che un’ applicazione pratica.
Dall’articolo del 1963 di Amartya Sen in poi, la letteratura ha presentato vari articoli
sull’argomento. Attualmente, il problema delle chiusure del modello non appare più centrale nel dibattito. Dopo una breve introduzione su cosa siano i CGE, il loro sviluppo e la loro struttura, è presentata una serie di esemplificative maquette con lo scopo di introdurre al concetto di chiusura, come essa influenzi i risultati e come questa scelta del modeller sia intimamente connessa ai fondamenti macroeconomici del sistema. Dopo la teoria, ci si sposta nel mondo reale analizzando con differenti modelli (Neoclassico, “Bastardo Keynesiano”, Strutturalista/Post- Keynesiano) e diverse chiusure per gli aggregati macroeconomici (risparmi privati, pubblici, e stranieri) l’impatto dell’accordo regionale SADC sull’economia mozambicana.
I modelli CGE per il Mozambico sono calibrati su una SAM del 2003 e sono svolti con l’ausilio di GAMS/MPSGE. I risultati dimostrano che la chiusura influenza i risultati stessi del modello per cui ognuno presenta una serie di raccomandazioni politiche per l’applicazione dell’accordo SADC.Modellers’ choice on closure rules affects a CGE model results and consequently its policy prescriptions. In this thesis, the aim is to detect and assess this issue, both through a theoretical discussion and an empirical application. Starting from Amartya Sen’s 1963 paper, literature presents many contributions on this topic. Currently, the closure rule problem is not central in the CGE debate. After a brief introduction on CGEs, their development and their structure, a series of simple maquette is presented. They have the exemplary role of introducing the concept of closures, explain how they affect final outcomes and how this modeller’s choice is strictly connected to the macroeconomic foundation of the economic system. After theory, we move into the real World analyzing through different models (Neoclassical, “Bastard Keynesian”, and Structuralist/ Post- Keynesian), and through different closure rules for macro- aggregates (private, public and foreign savings) the impact of the Regional Trade Agreement of SADC with respect to the Mozambican economy. The Mozambican CGE models are calibrated on a 2003 Social Accounting Matrix (SAM) and they are performed using GAMS/ MPSGE. Outcomes show that closure rules affect them and each model presents a set of policy prescription to implement the SADC agreement.
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Granello, Giulia <1996>. "Le Agevolazioni Fiscali: ristrutturazioni edilizie e novità Superbonus 110%." Master's Degree Thesis, Università Ca' Foscari Venezia, 2021. http://hdl.handle.net/10579/20372.
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la tesi inizierà con una panoramica generale introduttiva sulle Agevolazioni fiscali e le principali caratteristiche sotto un profilo economico e fiscale. si farà una panoramica delle principali tipologie di Agevolazioni analizzando gli effetti sul comportamento degli agenti economici e come incidono giornalmente su di essi. si proveranno a trovare i vantaggi e gli svantaggi che le agevolazioni comportano ai singoli individui e allo Stato.
poi si approfondiranno alcune agevolazioni, le ristrutturazioni edilizie, il bonus mobili e la novità degli ultimi anni, il Superbonus 110%.
verrà dedicato un capitolo a ciascuno e si descriveranno gli aspetti generali e le dinamiche fiscali che si devono seguire per poterne usufruire.
per ognuna verrà descritto chi può usufruirne, come poterne usufruire e quali interventi edilizi permettono di rientrare in queste agevolazioni.
per finire verrà dedicato un intero capitolo al Superbonus 110% e alle numerose novità che ha introdotto, cercando di approfondire alcune casistiche particolari.
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今泉, 和良, 征史 近藤, 勤. 川部, 直純 橋本, 香平 横井, 好規 長谷川, and 薫. 下方. "P-342 進行性の呼吸不全と多臓器転移を来した胸膜原発 epithelioid hemangioendothelioma (EHE) の1例." 日本肺癌学会, 2006. http://hdl.handle.net/2237/11039.
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DELLA, NOCE MATTEO. "Un modello VAR-GARCH multivariato per il mercato elettrico italiano." Doctoral thesis, Università Cattolica del Sacro Cuore, 2011. http://hdl.handle.net/10280/1108.
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E’ stato estesamente appurato che i mercati dell'elettricità mostrano mean-reversion e elevata volatilità dei prezzi. Questo lavoro utilizza un modello VAR-MGARCH al fine di cogliere queste caratteristiche presenti sul mercato dell'energia elettrica italiana (IPEX) e analizzare le interrelazioni esistenti tra le diverse regioni in cui il mercato è suddiviso. L’analisi è condotta sui prezzi giornalieri dal 1 ° gennaio 2006 al 31 dicembre 2008.
I coefficienti stimati dalle equazioni condizionali indicano che i mercati regionali sono abbastanza integrati e i prezzi regionali dell'energia elettrica possono essere adeguatamente previsti impiegando i prezzi passati di ciascun mercato zonale.
La volatilità e la cross-volatility sono significative per tutti i mercati, indicando la presenza di forti componenti ARCH e GARCH e la sostanziale inefficienza dei mercati. E’ inoltre evidente un’elevata persistenza della volatilità e della cross-volatility in tutti i mercati.
I risultati indicano inoltre che gli shock rilevati, sia nella volatilità, sia nei vari mercati, persistono nel tempo e che in ogni mercato la persistenza è più marcata quando è causata da innovazioni stimate sulle stesso mercato rispetto a shock stimati su altre aree. Questa persistenza descrive la tendenza delle variazioni dei prezzi a raggrupparsi nel tempo.It is commonly known that spot electricity markets show mean-reversion and high price volatility. This work employs a VAR-MGARCH model to capture these features in the Italian electricity market (IPEX) and analyze the interrelation existing among the different regions in which the market is divided. Daily spot prices from 1 January 2006 to 31 December 2008 are employed. The estimated coefficients from the conditional mean equations indicate that the regional markets are quite integrated and regional electricity prices could be usefully forecasted using lagged prices from either the same market or from the other areal markets. Volatility and cross-volatility spill-overs are significant for all markets, indicating the presence of strong ARCH and GARCH effects and market inefficiency. Strong persistence of volatility and cross-volatility are also evident in all local markets. The results also indicate that volatility innovations or shocks in all markets persist over time and that in every market this persistence is more marked for own-innovations or shocks than cross-innovations or shocks. This persistence captures the propensity of price changes of similar magnitude to cluster in time.
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DEVEZA, Michele Valadares. "Fungos e micotoxinas presentes em amostras de p?len de abelhas mel?feras do estado do Rio de Janeiro." Universidade Federal Rural do Rio de Janeiro, 2011. https://tede.ufrrj.br/jspui/handle/jspui/1173.
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Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
The bee?s pollen is a product rich in essential nutrients to feed the bees. In the hive it goes through chemical processes and becomes the bee?s bread, of extreme importance for the growth of larvae. Currently, the use of pollen in the human?s cuisine has been increasing for its therapeutic properties, and by its use in the food industry. To produce quality pollen the hive needs to be healthy, and bees, like any other animal, are susceptible to various diseases, parasites and predators, which can affect their development and productivity. The The Brazilian Sac Brood (BSB) is one of these diseases, which in Brazil affects honeybee larvae. In apiaries of Rio de Janeiro state, a high mortality of larvae with symptoms similar to the BSB is occurring, and until now, there is no definite conclusion regarding the etiologic agent of this disease in the state. Therefore, the objectives of this study was to clarify two issues: 1) provide a mycological research, through a study case, of bees? bread samples, adult bees and bees? pollen in areas affected and not affected by the BSB, aiming the elucidation of its etiologic agent, 2) verify the hygienic quality of dehydrated pollen samples sold in Rio de Janeiro state. A total of 48 samples of bees? bread, 15 of adult bees and 12 of pollen were collected from endemic regions of the BSB. The samples were collected in the months before, during and after the period of the disease. The dehydrated pollen samples were also purchased in commercial stores of the state, totaling 27 samples. Fungal species enumeration (cfu g-1) was through decimal dilution method using general culture media and selective ones. The isolation frequency and relative density of species were determined. The toxigenic profile of strains was determined, as also the detection and quantification of aflatoxin B1 in bread and bees? pollen. The results obtained from the study case samples showed that the bees? bread was the best vehicle of contamination for the hive. It was also observed a high fungal counts and the presence of mycotoxins and entomopathogenic fungi such as Aspergillus flavus, A. niger aggregate and A. fumigatus, which cause a decrease of the immune response, leaving them susceptible to various diseases, including the BSB itself. Pollen dehydrated samples showed a high fungal contamination with 92% of the samples in unsatisfactory hygienic conditions according to legal standards. A total of 25% of the A. flavus strains showed to be aflatoxigenic. The high percentage of unhygienic samples combined with the presence of a large variety of fungal contaminants, including mycotoxin-producing species, evidences that the legislation needs to be updated so that it can be safely commercialized. It is considered that the whole line production of bees? pollen, since its extraction until its storage should be monitored in order to minimize risks to human health.
O p?len ? um produto ap?cola rico em nutrientes essenciais para a alimenta??o das abelhas. Na colmeia ele passa por processos qu?micos e se torna o p?o de abelhas, de extrema import?ncia para o crescimento das larvas. Atualmente, o p?len ap?cola vem se destacando tamb?m na alimenta??o humana, tanto por suas propriedades terap?uticas, quanto pela sua utiliza??o na ind?stria aliment?cia. Para a produ??o de um p?len de qualidade a colmeia precisa estar sadia, e as abelhas, como qualquer outro animal, s?o suscept?veis a v?rias doen?as, parasitas e predadores, que podem prejudicar seu desenvolvimento e produtividade. A Cria Ensacada Brasileira (CEB) ? uma dessas doen?as, que no Brasil acomete larvas de abelhas mel?feras. Em api?rios do estado do Rio de Janeiro v?m ocorrendo uma alta mortalidade de larvas com sintomatologia semelhante a da CEB, e ainda n?o h? uma conclus?o definitiva acerca do agente etiol?gico desta doen?a no estado. Portanto, os objetivos desse estudo visam esclarecer duas problem?ticas: 1) avaliar, atrav?s de estudo de caso, amostras de p?o de abelhas, abelhas adultas e p?len ap?cola em regi?es atingidas e n?o atingidas pela CEB, diagnosticando a presen?a de fungos, visando fornecer informa??es importantes para a elucida??o do agente etiol?gico da CEB no estado do Rio de Janeiro; 2) verificar a qualidade higi?nica das amostras de p?len desidratado comercializado no estado do Rio de Janeiro. Um total de 48 amostras de p?o de abelhas, 15 de abelhas adultas e 12 de p?len ap?cola, foram adquiridas de regi?es end?micas da CEB. As coletas ocorreram nos meses que antecederam, durante e ap?s o per?odo da doen?a. J? as amostras de p?len desidratado foram adquiridas no com?rcio do estado, totalizando 27 amostras. A enumera??o da micobiota (ufc g-1) foi realizada atrav?s do m?todo de dilui??o decimal com semeadura em placas utilizando-se meios de cultivo gerais e seletivos. Foram determinadas a freq??ncia de isolamento dos g?neros f?ngicos e a densidade relativa das esp?cies. Foi determinado o perfil tox?geno de cepas isoladas e foi feita a detec??o e quantifica??o de aflatoxina B1 no p?o de abelhas e p?len ap?cola. Nos resultados obtidos das amostras do estudo de caso, o p?o de abelhas mostrou ser o melhor substrato para o ve?culo de contamina??o para a colmeia. Observou-se tamb?m altas contagens f?ngicas, al?m da presen?a de micotoxinas e fungos entomopatog?nicos, como Aspergillus flavus, A. niger agregados e A. fumigatus o que favorece a queda da imunidade desses insetos deixando-os suscet?veis a diversas doen?as, entre elas a pr?pria CEB. Em rela??o ao p?len desidratado, os resultados mostraram uma alta carga f?ngica com 92% das amostras em condi??es higi?nicas insatisfat?rias segundo os padr?es legais consultados e 25% das cepas de A. flavus mostraram-se potencialmente produtoras de aflatoxinas. Os alt?ssimos percentuais de reprova??o das amostras para a qualidade higi?nica, aliado ? presen?a de grande variedade de fungos contaminantes, incluindo esp?cies produtoras de micotoxinas, ? uma evid?ncia de que a legisla??o necessita ser atualizada, para que este produto possa ser comercializado com seguran?a. Considera-se que toda a linha de produ??o do p?len ap?cola, desde sua coleta at? o armazenamento deva ser monitorada, a fim de minimizar os riscos para a sa?de humana
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Tso, Wing Bo. "A comparative study of gender representations in Philip Pullman’s His Dark Materials and its Chinese translation." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/1163/.
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Philip Pullman’s His Dark Materials has caused controversy as well as enjoyed great popularity among readers worldwide. Its influence has created a great impact in the field of children’s literature. The purpose of this thesis is two-fold. Firstly, the thesis analyzes gender representations in Pullman’s trilogy in the context of how he rewrites female archetypes through the subversive re-inscription of Eve, the invention of daemons, the reinvention of ‘femme fatale’, and the new portrayal of Gypsy women. Secondly, the thesis aims at comparing and examining how gender representations in the source text are translated, transformed or / and manipulated in its Chinese translation. With reference to Chinese gender ideology, which includes the Chinese concept of the ying-yang polarities, Buddhist notions of gender, the notion of the femme fatale, and the stereotypical image of Chinese grannies, the syntactic and semantic alterations made by the Chinese translator are investigated. Issues regarding how Chinese gender views may influence and alter the translation product are discussed in detail. By studying the similarities and differences in gender representations between the texts, the thesis attempts to shed light on the gender ideology of both English and Chinese contemporary cultures.
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野口, 雅弘, 和良 今泉, 勤. 川部, 芳嗣 堀尾, 尚. 若山, 徹. 原, 直純 橋本, 好孝 関戸, 好規 長谷川, and 薫. 下方. "P-18 CD40リガンド遺伝子及びIFNγ遺伝子導入肺癌細胞による生体内での抗腫瘍免疫の誘導." 日本肺癌学会, 2001. http://hdl.handle.net/2237/11037.
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Storbeck, Karl-Heinz. "The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat." Thesis, Link to the online version, 2008. http://hdl.handle.net/10019.1/1101.
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渡辺, 篤., 好規 長谷川, 英雄 坂, and 薫. 下方. "P-150 肺癌培養細胞株に対するSNー38と放射線の併用効果 : 併用時期についての検討." 日本肺癌学会, 1993. http://hdl.handle.net/2237/11035.
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Meier, Thomas W. "Zur Wirkungsweise von P₄₅₀₋abhängigen Enzymen : mechanistische und stereochemische Untersuchungen mit deuterierten Substraten /." Zürich, 1995. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=11093.
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Pedra, Alysson Correia. "A crise financeira de 2008 e seus impactos na economia brasileira: uma análise sob a perspectiva de Minsky." reponame:Repositório Institucional da UFES, 2014. http://repositorio.ufes.br/handle/10/1100.
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Diante do colapso financeiro de 2008, este trabalho retoma a teoria econômica proposta por Hyman P. Minsky com o objetivo de esclarecer as circunstâncias que propiciaram uma crise financeira tão profunda. A estrutura analítica de Minsky é marcada pela Hipótese da Instabilidade Financeira, a qual aponta para fatores endógenos ao próprio sistema capitalista como o principal causador de instabilidades financeiras. Este processo, caracterizado principalmente por um avanço desfavorável no nível de endividamento dos agentes, constrói um ciclo de estágios que pode se desenvolver para uma crise financeira ou um colapso sistêmico, definidos como “Momento Minsky” e “Colapso Minsky”. Este cenário descrito por Minsky, também analisado à luz de teorias mais recentes como as de Gary A. Dymski e Alessandro Vercelli, é conhecido por “ciclo minskyano”. Ao adotar estes preceitos da análise teórica de Minsky, é possível visualizar como o processo de desregulamentação e fragilização financeira dos Estados Unidos nas décadas de 1980 e 1990 proveram condições para a crise do subprime e, posteriormente, o colapso financeiro de 2008. De maneira similar, é possível observar que a análise teórica de Minsky também é aplicável à crise que afeta a economia brasileira no final de 2008. A fragilização financeira que se inicia no Brasil poucos anos antes da crise, acentuada no setor exportador de commodities, cria a condição para o “momento Minsky brasileiro”, demonstrando que apesar das falhas da análise teórica de Minsky, que supõe uma economia fechada com características da economia estadunidense, é possível visualizar uma relação de causa e efeito da recente crise financeira com a teoria minskyana.
Having in sight the world's financial collapse of 2008, this dissertation takes up the economic theory proposed by Hyman P. Minsky aiming to clarify the circumstances that led to so deep financial crisis. Minsky’s analytical framework was built on the Financial Instability Hypothesis, which identifies in the internal dynamics of the capitalistic system as the main cause of its financial upswings and downturns. This process, characterized by the progressive indebtedness of economic agents, builds itself in a cycle of predefined stages that often reach the apex of a financial crisis or a systemic collapse, best known in the literature, respectively, as “Minsky Moment” and “Minsky Collapse”. Minsky's overall approach to the financial modus operandi of modern economies was complemented by the more recent contributions of Gary A. Dymski and Alessandro Vercelli, who explored the idea of a Miskian cycle. By reviewing these theories in some detail, it is possible to see how the process of the deregulation and financial weakness of the United States in the 1980s and 1990s paved the way to the subprime crisis and, later, of the 2008 financial collapse. Similarly, is possible to observe that Minsky’s theory is also applicable to the Brazilian financial crisis in 2008. The financial fragility that began to develop in Brazil some years prior to the crisis, mainly in the commodities export sector, created the conditions for the “Brazilian Minsky’s moment”, demonstrating that despite some limitations of Minsky's analysis, such as the assumption of a closed economy, his theory offers important insights not only into the operation of financial markets in fully advanced economies but also in the emerging ones as well.
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ALIZZI, ANTONIO. "PROFESSIONALITA', RUOLO E FUNZIONI DEL DIRETTORE SANITARIO. AMBITI DI MANAGERIALITA' IN TRE ORGANIZZAZIONI AD ELEVATA COMPLESSITA': ISTITUTO CLINICO HUMANITAS, ISTITUTO EUROPEO DI ONCOLOGIA, OSPEDALE MAGGIORE POLICLINICO." Doctoral thesis, Università Cattolica del Sacro Cuore, 2011. http://hdl.handle.net/10280/1106.
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Nell’ambito delle professioni mediche, quella del direttore sanitario aziendale risulta poco indagata. La ricerca – di tipo qualitativo, condotta sui direttori sanitari di tre ospedali di Milano – si propone di investigare questo ruolo professionale a partire dalle novità registrate nell’ultimo ventennio e dagli obiettivi che si pone nel proprio campo.
L’ipotesi è che la figura esaminata abbia subito tali e profonde mutazioni per cui:
a) in superficie, sono cambiate le competenze fondamentali per l’esercizio efficace del ruolo; in particolare, l’attività “produttiva” è stata affidata al “direttore medico di presidio” mentre la programmazione e il coordinamento strategico al direttore sanitario;
b) in profondità, compromesse e dissolte quelle caratteristiche che Freidson considerava tipiche della professione, il direttore sanitario è andato costruendosi una nuova professionalità, caratterizzata dai saperi tecnici tradizionali, ma anche da nuove competenze manageriali.
Viene ripercorso normativamente e storicamente il cambiamento intervenuto nel ruolo, fino a delinearne i nuovi ambiti di managerialità. Individuare tali competenze è utile per: includerle, mediante percorsi educativi specifici, nel fabbisogno formativo professionale, leggerne il ricorso e lo sviluppo, in una chiave isomorfica normativa, come funzionale allo stesso direttore sanitario per attivare/controllare il cambiamento, tanto nell’ospedale, quanto nel campo organizzativo della salute.Within the scope of medical professions, the role of the chief medical officer is not so much investigated. The present quality-type research has been carried out on the activity of chief medical officers in three hospitals in Milan and aims to investigate this profession starting from the innovations it underwent during the last twenty years and from the goals it intends to reach. The hypothesis we formulated is that the role we are analyzing has undergone such deep changes that: a) on the surface, fundamental skills required for an efficient practice of this profession have changed; in particular, the “productive” activity has been entrusted to the hospital medical director, while programming and strategic coordination has been ascribed to the chief medical officer; b) in depth, since the characteristics considered as typical by Freidson were compromised and hence dissolved, chief medical officers have been acquiring a new professionalism, marked by traditional knowledge but also by new managerial skills. Changes occurred in this profession have been studied in the present dissertation both from a regulatory and from an historical point of view, in order to trace out the new scope of its managerial activities. Detecting skills is important: for including them among the educational needs of this profession to be acquired thanks to specific training paths, for tracing their recurrence and development in the frame of a normative isomorphism, which would be useful for the chief medical officer himself in order to start/monitor the changes as much in the hospital as in the whole organizational health field.
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STEFANIZZI, PASQUALE. "Il microcredito in Italia: analisi dei fattori critici di successo." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2009. http://hdl.handle.net/2108/1107.
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Il microcredito ha ad oggetto il sistema di concessione di prestiti a persone rientranti nel circuito dei c.d. soggetti “non bancabili, ossia sprovvisti di ogni e qualsivoglia garanzia reale e/o personale, e l’implementazione di una vasta e variegata gamma di servizi finalizzati all’avvio di un vero e proprio circuito finanziario tipico raccolta-impieghi (Yunus, 1998; La Torre, Vento, 2005).
Lo scopo principale del lavoro è quello di comprendere se il microcredito in Italia possa rappresentare un’opportunità finanziaria da implementare su più larga scala al fine di migliorare l’attitudine al credito degli active poors del Paese. L’indirizzo di approfondimento è mirato all’individuazione di specifici e ben determinati target, sia dal lato della domanda (gli immigrati) che dal lato dell’offerta di microcredito (il sistema di credito cooperativo).
L’origine dell’interesse verso questo ambito di studio è da individuarsi nella gran quantità di autori che a vario titolo si sono misurati con l’argomento proponendo modelli e possibilità operative di pregevole spessore ed interesse.
Il tentativo di sintetizzare in una main research question quanto sinora esposto induce a proporre la seguente domanda di ricerca: “gli immigrati (dal lato della domanda) ed il sistema di cooperazione di credito (dal lato dell’offerta) possono rappresentare gli specifici attori cui indirizzare le attenzioni della ricerca scientifica al fine di creare, in Italia, un prodotto di microcredito dall’elevata standardizzazione ed utilizzabile per espandere la portata dei suoi benefici?”.
Relativamente alla metodologia adottata, si evidenzia che la segmentazione dello studio per aree di ricerca, diverse nell’oggetto ma comuni nello scopo, ha imposto l’adozione di tecniche di approccio integrate quali-quantitative, precedute sempre da una rassegna della letteratura esistente, nazionale ed internazionale.
In particolare, nella sezione dedicata alla ricerca empirica (composta da due indagini: la prima avente l’obiettivo di comprendere quale sia l’apertura delle BCC al microcredito ed agli immigrati, la seconda indirizzata alle organizzazioni di microcredito che operano attualmente in Italia allo scopo di determinare il posizionamento di questo innovativo strumento di credito nel più complesso panorama finanziario nazionale in termini spaziali, temporali, di volumi e natura delle operazioni) sono state impiegate metodologie statistiche campionarie di analisi quantitativa che utilizzano il questionario come strumento di raccolta dati. I data base sono stati opportunamente ideati e costruiti, fornendo alla ricerca spunti di interessante originalità. Al fine di rappresentare adeguatamente le dinamiche che interagiscono nei settori di volta in volta indagati, si è ritenuto opportuno utilizzare congiuntamente diverse tecniche di elaborazione ed interpretazione dei risultati, avvalendosi di strumenti riconducibili alla cluster analisys, all’analisi delle serie storiche e all’analisi descrittivo-esplorativa.Microcredit is concerned with the system of granting loans to people falling within the realm of the so-called ‘unbankable’, namely those devoid of any kind of collateral and / or personal guarantee, and the deployment of a wide and varied range of services aimed at the creation of a real and tangible investment-funding network (Yunus, 1998; The Torre, Wind, 2005). The main purpose of this study is to understand if microcredit in Italy offers an opportunity to deploy financing on a larger scale to improve the willingness to extend credit to the country’s active poor. The aim of this study is to identify specific and clearly defined targets, both on the side of the applicant (the immigrants) and that of the microcredit provider (the credit cooperative system). Interest in this area of study came about after selecting from a large number of authors who, for various reasons, have tackled this issue by proposing models and possible solutions of particular note. An attempt to summarize this into one main research question, as outlined so far, leads us to propose the following question: "Could immigrants (on the request-side) and the credit cooperative system (on the supply-side) represent the actual parties which will direct the attention of scientific research in creating a microcredit product of high standards and usability in order to expand the range of benefits?". With regard to the methodology used, it appears that the segmentation of the study into areas for research, different in scope but common in goal, has required the adoption of quali-quantitative integrated approach techniques which always preceded by a review of the existing national and international literature. In particular, in the section devoted to empirical research, statistical sampling methods for quantitative analysis were used through questionnaires as a means of collecting data (consisting of two surveys: the first, aimed at understanding how to introduce the Credit Cooperative Banks (BCC) to microcredit as related to immigrants, the second addressed at microcredit organizations that are currently operating in Italy, in the more complex, domestic financial landscape in order to determine the placement of this innovative credit tool in terms of distribution, timing, quantity and the nature of operations). Furthermore, the databases were properly designed and constructed, providing interesting and original ideas for the research. In order to adequately represent the dynamics under investigation, that occur in the field from time to time, it was considered appropriate to simultaneously use different techniques for processing and interpreting results, making use of tools associated with cluster analysis, time-series analysis and descriptive-exploratory analysis.
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Marconi, Eduardo Salgado. "Parque linear ?guas do Camandocaia em Amparo - SP: agentes e processos na cria??o, apropria??o e qualifica??o dos espa?os p?blicos." Pontif?cia Universidade Cat?lica de Campinas, 2018. http://tede.bibliotecadigital.puc-campinas.edu.br:8080/jspui/handle/tede/1130.
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Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq
The urban planning tool Parque Linear has been identified in Brazil as a sustainable measure of use and occupation of urban areas of valley bottom in the environmental, social, economic and cultural spheres. This work proposes to carry out an analysis of how the process of urban requalification took place in the municipality of Amparo, in the State of S?o Paulo, having as object of study the Linear Park ?guas do Camandocaia. It is intended to raise the trajectory, the scope and limits of the urban interventions implemented along the Camandocaia River. In its first part, it proposes to present, through periodization - via bibliographical research and analysis of historical documents - the historical process of urban evolution and its influence on issues related to the theme. In the second part, it points out interferences as the basis of the transformations in the new urban design from legal and political dimensions. In the third part, the presentation of the object itself in the technical aspects of the urban and architectural projects, the financial resources raised until the bidding processes, the execution of the works along the river. The fourth part deals with the concepts related to the production of public spaces and the sphere of public life as fundamental supports for the improvement of the quality of urban life. In the fifth and last part, in the correlation of the quality attributes of several authors for the analysis of the spatial quality of the Linear Park.
O instrumento urban?stico Parque Linear tem sido apontado, no Brasil, como medida sustent?vel de uso e ocupa??o das ?reas urbanas de fundo de vale nos ?mbitos ambiental, social, econ?mico e cultural. Este trabalho prop?e realizar uma an?lise de como se deu o processo de requalifica??o urbana no munic?pio de Amparo, no Estado de S?o Paulo, tendo como objeto de estudo o Parque Linear ?guas do Camandocaia. Pretende-se levantar a trajet?ria, os alcances e limites das interven??es urbanas implementadas ao longo do rio Camandocaia. Em sua primeira parte, prop?e apresentar por meio da periodiza??o ? via pesquisas bibliogr?ficas e an?lise de documentos hist?ricos ? o processo hist?rico da evolu??o urbana e sua influ?ncia em quest?es relacionadas ao tema. Na segunda parte, aponta inger?ncias como base das transforma??es no novo desenho urbano a partir de dimens?es legais e pol?ticas. Consta, na terceira parte, a apresenta??o do objeto em si nas quest?es de ordem t?cnica dos projetos urban?stico e arquitet?nico, dos recursos financeiros captados at? os processos licitat?rios, da execu??o das obras ao longo do rio. A quarta parte aborda os conceitos vinculados ? produ??o dos espa?os livres p?blicos e da esfera de vida p?blica como suportes fundamentais ao incremento da qualidade de vida urbana. A quinta parte apresenta a correla??o dos atributos de qualidade segundo diversos autores para a an?lise da qualidade espacial do Parque Linear.
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Newton, Mark A. "Alloy effects in catalysis : the structure and reactivity of the CuPd[85:15]{110}p(2x1) surface." Thesis, University of Liverpool, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240235.
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Renard, Frédéric. "Étude de la structure du nucléon via la photoproduction de mésons pseudoscalaires à GRAAL : mesure des sections efficaces et des asymétries de faisceau des réactions [gamma]+p->p+[pi]°et [gamma]+p->p+[éta] pour E[nu] compris entre 550 et 1100 MeV." Grenoble 1, 1999. http://www.theses.fr/1999GRE10270.
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L'experience graal est consacree a la comprehension de la structure dynamique du nucleon et de ses etats excites, les resonances baryoniques. L'etude experimentale des reactions de photoproduction de mesons sur le nucleon est accomplie a l'aide d'un faisceau de photons polarises et d'un multi-detecteur 4. Le faisceau est produit par retrodiffusion compton de photons laser sur les electrons de 6 gev du synchrotron europeen a grenoble (l'esrf). Cette these porte sur l'analyse des premieres donnees acquises par la collaboration graal. Apres la caracterisation du faisceau et le calcul de la dose de photons etiquetes, l'analyse decrit les techniques d'identification des reactions de photoproduction des mesons 0 et. Les performances du systeme de detection, resolutions et efficacites, sont determinees a partir des mesures experimentales et des simulations. Les prises de donnees realisees en 1997 ont permis d'etudier les reactions p 0p et p p dans une gamme d'energie des photons allant de 550 a 1100 mev. L'analyse complete de ces deux canaux a ete effectuee et des resultats finaux pour la section efficace d/d et l'observable de polarisation ont ete obtenus. L'ensemble des corrections aux donnees ainsi que l'evaluation des erreurs systematiques sont presentes en detail. L'asymetrie faisceau obtenue avec une polarisation lineaire des photons, a ainsi ete mesuree pour la premiere fois dans la production du. La section efficace du meme processus et les mesures obtenues pour la production de 0 ameliorent grandement la precision et la couverture cinematique de la base de donnees existante. Les observables de polarisation sont sensibles a des contributions tres petites, difficiles a discerner dans les mesures de section efficace. Ainsi, le rapport de branchement de la resonance n*(1520) dans le canal a pu etre determine avec une bonne precision: b = / t o t = (0,08 0,01)%. Ces resultats sont selectifs pour les modeles des hadrons et contribuent ainsi a l'amelioration de notre connaissance des baryons et des mesons.
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ROSENBLATT, THOMAS. "Structures uniaxiales p(nx1) dans le systeme pb/cu(110) : etude par simulation des phases non alliees et alliees." Paris 6, 1996. http://www.theses.fr/1996PA066365.
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On presente une etude de simulation de la serie des structures uniaxiales p(nx1), avec n=4, 17, 13, 9, 14, 19, 5, des couches bidimensionnelles de plomb sur un substrat (110) de cuivre, aux taux de recouvrement proches de la monocouche. Ces structures ont ete mises en evidence anterieurement par differentes techniques experimentales. Nous avons etudie les structures non alliees et alliees par deux techniques de simulation: relaxation statique, (minimisation de forces interatomiques au zero absolu) et simulation monte carlo a temperatures elevees. Des potentiels de paires et des potentiels a n corps du type liaison forte ont ete employes. Les configurations d'energie potentielle minimale des structures non alliees p(4x1) et p(5x1) ont ete determinees par relaxation statique. L'isotherme d'adsorption, taux de recouvrement versus pression d'etalement, a la temperature du zero absolu a ete calculee pour les structures p(nx1) non alliees a l'aide d'un modele issu du modele de frenkel-kontorova. Ces calculs n'ont pas permis de demontrer la stabilite meme relative des structures intermediaires a la p(4x1) et la p(5x1). Un essai de calcul de l'energie libre de ces memes structures a temperatures superieures au zero absolu par une integration thermodynamique, n'a pas change les conclusions des etudes a 0 k. Dans une derniere partie, les structures d'equilibre de la couche de plomb sur la face (110) du cuivre, determinees par le calcul des bilans d'energie potentielle accompagnant la transformation des couches non alliees en alliages de surface, ont permis d'etablir la stabilite de phases alliees a bas et a haut taux de recouvrement, en accord avec des resultats experimentaux recents
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Aramayo, Perla Julio. "STENZLER. ADOLF FRIEDRICH. Elementarbuch der Sanskrit-Sprache (Grammatik. Texce Woertebuch). Fortgefuehrt von Richard Pischel. Umgearbeitet van Karl F. Geldner. Vierzehnte Aufagle durchgesehen und ergaenzt von Samarendranath Biswas. Berlin. Verlag Alfred Toepelmann, 1960; viii, 110 p." Pontificia Universidad Católica del Perú, 2017. http://repositorio.pucp.edu.pe/index/handle/123456789/114407.
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Amorim, Maria Helena Rocha. "Efeito de estratégias de rega deficitária no desenvolvimento vegetativo e produção de Loureiro/ 1103-P na Região Demarcada dos Vinhos Verdes." Master's thesis, 2014. https://repositorio-aberto.up.pt/handle/10216/78278.
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Amorim, Maria Helena Rocha. "Efeito de estratégias de rega deficitária no desenvolvimento vegetativo e produção de Loureiro/ 1103-P na Região Demarcada dos Vinhos Verdes." Dissertação, 2014. https://repositorio-aberto.up.pt/handle/10216/78278.
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Clearfield, Howard M. "Low-energy electron diffraction studies of defect structures and ordering kinetics at p(lxl) surfaces GaAs(110) and GaAs(110) p(lxl)-Sb /." 1985. http://catalog.hathitrust.org/api/volumes/oclc/12098997.html.
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Thesis (Ph. D.)--University of Wisconsin--Madison, 1985.Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 442-451).
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Monyai, Florina Semakaleng. "Establishment of interaction partners of Plasmodium falciparum heat shock protein 70-x(PfHsp 70-x)." Diss., 2018. http://hdl.handle.net/11602/1113.
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MSc (Biochemistry)Department of Biochemistry
Plasmodium falciparum is a unicellular protozoan parasite that causes malaria in humans. The parasite is passed to humans through mosquito bites and migrates to the liver before it infects host erythrocytes. It is at the erythrocytic stage of development that the parasite causes malaria pathology. Malaria is characterized by the modification of host erythrocytes making them cytoadherent. This is as a result of formation of protein complexes (knobs) on the surface of the erythrocyte. The knobs that develop on the surface of the erythrocyte are constituted by proteins of host origin as well as some proteins that the parasite ‘exports’ to the host cell surface. Nearly 550 parasite proteins are thought to be exported to the infected erythrocyte. Amongst the exported proteins is P. falciparum heat shock protein 70-x (PfHsp70-x). Hsp70 proteins are known to maintain protein homeostasis. Thus, the export of PfHsp70-x may be important for maintaining protein homeostasis in the host cell. PfHsp70-x is not essential for parasite survival although is implicated in the development of parasite virulence. This is possibly through its role in facilitating the trafficking of parasite proteins to the erythrocyte as well as supporting the formation of protein complexes that constitute the knobs that develop on the surface of the infected erythrocyte. The main objective of the current study was to investigate protein interaction partners of PfHsp70-x. It is generally believed that PfHsp70-x interacts with various proteins of human and parasite origin. Potential candidate interactors include its protein substrates, Hsp70 co-chaperones such as Hsp40 members, and human Hsp70-Hsp90 organizing protein (hHop). The establishment of the PfHsp70-x interactome would highlight the possible role of PfHsp70-x in the development of malaria pathogenicity. Based on bioinformatics analysis, PfHsp70-x was predicted to interact with some exported P. falciparum Hsp40s, hHop and human Hsp90 (hHsp90). Recombinant forms of PfHsp70-x (full length and a truncated form that lacks the C-terminal EEVN motif implicated in co-chaperone binding) were expressed in E. coli BL21 Star (DE3) cells. Recombinant hHop and hHsp70 were expressed in E. coli JM109 (DE3) cells. The proteins were successfully purified using nickel affinity chromatography. Co-affinity chromatography using recombinant PfHsp70-x and immuno-affinity chromatography using PfHsp70-x specific antibody did not confirm the direct interaction of PfHsp70-x with human Hop. However, the direct interaction of hHop and PfHsp70-x has previously been validated in vitro and the current bioinformatics data support ii the existence of such a complex. PfHsp70-x was not stable in the cell lysate that was prepared and this could explain why its interaction with hHop could not be ascertained. However, taken together the evidence from a previous independent study, and the predicted interaction of PfHsp70-x with human chaperones suggests cooperation of chaperone systems which possibly facilitates the folding and function of parasite proteins that are exported to the infected erythrocyte.
NRF
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Chen, Wan-Li, and 陳宛勵. "On Three-Dimensional Stress Altered Hole Inversion-Layer Mobility in (001) and (110) p-MOSFETs." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/56506337595782478085.
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碩士國立交通大學
電子研究所
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Hole inversion-layer mobility under three-dimensional stresses in (001)/<110> and (110)/<110> of p-MOSFETs is investigated by using a self-consistent six-band k‧p solver. The three significant scattering mechanisms are included: optical phonon scattering, acoustic phonon scattering, and surface roughness scattering with the screening effect taken into account. This leads to a clearer physical insight into 3-D-stress-induced hole mobility change in terms of three parts: (1) effect of strained k‧p deformation potentials a, b, and d; (2) phonon-limited and/or surface-roughness-limited mobility change; and (3) scattering-time-limited and conductivity-effective-mass-limited mobility change. Finally, the conclusions indicate that (1) for the effect of the hole mobility change, a is weak, b is moderate, and d is strong, particularly for the uniaxial compressive stress in the <110> direction. The experimentally-calibrated values are: a = 2.46eV, b = -1.6 ~ -2.1eV, and d = -3.1eV; (2) the phonon-limited mobility change is more stress-sensitive than the surface-roughness-limited one; and (3) the mobility change ratio can be related to the reversely proportional conductivity effective mass and density-of-states effective mass in the absence of the surface roughness mobility change.
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Wang, Siou Ying, and 王琇塋. "Correlations Between Bulky Non-natural Amino Acid Substitution and Anti-endotoxin Activity in the Antimicrobial Peptide P-113." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/q4sw7y.
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Toofan, Jahansooz. "Development of new data collection and analysis techniques for low energy electron diffraction and their application to the Mo(110)-p(2x2)-S and Al���O��� (0001) systems." Thesis, 1997. http://hdl.handle.net/1957/34429.
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Konrad, Stephanie. "Interaktion der FO Statoruntereinheiten a und b der ATP-Synthase aus Escherichia coli." Doctoral thesis, 2002. https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2002040519.
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Interaktion der FO Statoruntereinheiten a und b der ATP-Synthase aus Escherichia coli
Die ATP-Synthase nimmt im Energiestoffwechsel vieler Organismen eine zentrale Stellung ein und ist ubiquitär in strukturell und funktionell homologer Form bei eukaryotischen Zellen in der inneren Mitochondrienmembran, der Thylakoidmembran von Chloroplasten und in der Cytoplasmamembran von Prokaryoten zu finden. Besonders zwischen F-, V- und A-Typ ATPasen bestehen strukturelle Ähnlichkeiten im Aufbaus des Gesamtenzyms aus zwei großen Subkomplexen. Darüber hinaus weisen die F-Typ ATPasen aller Organismen hohe Sequenzhomologien auf, welche sich auch in strukturellen Gemeinsamkeiten widerspiegeln.
Als "Modellenzym" dient die FOF1 ATPase aus dem Enterobakterium Escherichia coli. Es setzt sich aus acht funktionell verschiedenen Untereinheiten zusammen, die unter Hydrolysebedingungen relativ zueinander rotieren. Die Unterteilung der Enzymstruktur in Rotor (g e -c-Oligomer) und Stator (a 3b 3d ab2) erfordert das Vorhandensein einer stabilisierenden Struktur, dem sog. "second stalk". Im Hinblick auf den Mechanismus der rotierenden ATP-Synthase und dem Modell der elastischen Kopplung erscheint die Untereinheit b geeignet, um die durch das g e -c-Oligomer aufgebaute Rotationsspannung zu speichern. Wie die beiden b Untereinheiten mit den anderen FO Untereinheiten a bzw. c interagieren ist weitgehend unbekannt.
In der vorliegenden Dissertation wurden die Untereinheiten a und b auf mögliche Interaktionsstellen mit anderen Enzymuntereinheiten mittels genetisch eingefügte Cysteine und anschließender chemischer Quervernetzung untersucht.
In der hier vorgestellten Arbeit konnte gezeigt werden, dass es mit dem Nulllängen Cross-linker Cu(1,10-Phenanthrolin)2SO4 [CuP] in der Region bP28C-bE39C möglich ist, Quervernetzungen zur Untereinheit a zu erzeugen. Mit den heterobifunktionellen Cross-linkern Benzophenon-4-maleimid [BPM] und N-[4-(p-Azidosalicylamido)butyl]-3´-(2´-pyridydithio)propionamid [APDP] vergrößert sich diese Region. Dabei sind die a-b Interaktionen in einer gewissen Periodizität (bP28C, bL29C, bM30C, bA31C, bK38C und bE39C) zu beobachten, was für eine Beteiligung beider b Untereinheiten spricht.
Neben dem immunologischen Nachweis durch Antikörper, konnte auch über ein N-terminales Polyhistidinmotiv (His12) gezeigt werden, dass eine Interaktion zwischen den Untereinheiten a und b ausbildet wird. Der aN-His12-b Cross-link kann mittels Ni-NTA Affinitätschromatographie aufgereinigt werden.
b-Dimerisierungen konnten für die Reste bS60C, bL65C und die Region bY24C-bA45C nachgewiesen werden. Der relative Abstand der b Untereinheiten zueinander nimmt dabei in ihrem Verlauf vom Cytoplasma in Richtung Membran zu, wie mit den Cross-link Reagenzien CuP, BPM und APDP gezeigt werden konnte.
Ausgehend von der Untereinheit a konnten für die Reste aS27C, aN33C, aA130C, aG173C, aP182C, aN184C, aS202C und aG227C ebenfalls CuP vermittelte Quervernetzungen mit der Untereinheit b nachgewiesen werden. Die Reaktion an der Position aS27C weist auf eine cytoplasmatische Lokalisation des N-Terminus hin, die in einem 6 Transmembran-Sekundärstrukturmodell vorgeschlagen wird.
Mit BPM konnte die Nähe der Aminosäuren aN33C und aP182C zum c-Oligomer gezeigt werden.