Dissertations / Theses on the topic '069902 Global Change Biology'

The College Woods, located in Williamsburg, VA, is a natural preserve of approximately 960 acres owned by the College of William & Mary. The Woods supports a relatively diverse flora in a mature coastal-plain forest which has been under long-term biological study by members of the College. This thesis contributes to the ongoing investigation of the Woods by documenting floristic and vegetation changes that have occurred over the last 45 years amidst a rising and uncontrolled white-tailed deer population. Three main research questions are addressed: (1) How has floristic diversity, composition, and species abundance changed since the last floristic survey in 1989? (2) Under the assumption of chronic browse by white-tailed deer, are there species-specific plant traits that associate with a suite of declining species? and (3) What is the estimated forest successional trajectory indicated by a 2015 quantitative analysis of the diversity, composition, importance, and browse rates in 19 long-term permanent plots? For the floristic analysis, 297% more effort was required in 2015 to find 7% fewer species than in the last floristic survey of 1989, indicating a decline in the abundance of populations, making their rediscovery more difficult. Over the last 45 years, 745 vascular plant species have been documented in the Woods. One hundred and twenty-six species were newly reported in 2015, while 196 previously-reported species were not relocated. The turnover of species is consistent with the species-time relationship and was especially prominent in early successional open habitats. Assessments of changes in relative abundance showed that 46% of the species had declined in abundance. This appears to be driven by an overabundant white-tailed deer population, but no plant trait previously hypothesized to confer vulnerability to browse by white-tailed deer showed a signficant association with the set of declining species. It is hypothesized that because the deer population has been overabundant for 20 years, any trait-based associations that may have once existed would have become obscured over time as browse intolerant species were reduced and deer switched to less-preffered plant material. Deer-browse data on less preferred plants such as Fagus grandifolia, Polystichum acrosticoides and Ilex americana supports this hypothesis. Nineteen permanent plots erected in 2003 were sampled for vegetation analysis immediately after Hurricane Isabel in 2004 and then resampled in 2015. Analysis showed little change in the large tree and small tree size classes, but there was significant change in the sapling size class. In the sapling size class, average stem density and species diversity significantly decreased between 2004 and 2015, or did not show the expected regeneration patterns in areas severely hit by the hurricane. An analysis of deer browse in these plots showed that nearly 60% of all vegetation was browsed. This is expected to slow down the rate of succession and alter forest composition, possibly resulting in a beech-dominated forest with very little understory. A management plan designed to allow the flora of the College Woods to recover from chronic deer browse was written and recommends controlling the white-tailed deer population through yearly managed hunts.

1. With freshwater ecosystems worldwide at significant risk from global change, there is an urgent need to understand the processes involved and to develop adaptive responses. Riparian management might offer a means of increasing resilience to global change in headwaters, but evidence is scarce. This thesis investigates the potential effects of riparian management on the storage, processing and downstream export of resource subsidies – dominantly as terrestrial litter – that enter streams from the riparian zone. 2. In a large scale field study over four years, natural and experimental systems were used to test the hypothesis that riparian woodlands enhance stream ecosystem resilience to climatically mediated changes in flow regimes. Specific work included assessments of benthic organic matter stocks and export in contrasting catchments (broadleaf woodland, conifer plantations or sheep-grazed moorland), flow manipulations in mesocosms, and a large-scale field experiment simulating riparian broadleaved tree planting. 3. Standing stocks of particulate organic matter (POM) were influenced by flow regime, and declined following larger and longer flow-events, but event frequency had no apparent impact. Experimental data showed also that coarse fractions of POM in transport were significantly elevated in the early stages of simulated floods. 4. Despite flow effects on POM dynamics, streams bordered by broadleaves maintained consistently higher standing stocks of POM than conifer or moorland streams. Broadleaved streams also transported the highest concentrations of carbon in the form of high-quality FPOM. Leaf litter additions of stream channels did not reproduce these effects, possibly because the scale was insufficient to mimic real riparian woodlands. 5. While predicted flow changes under a warmer climate might affect the storage and flux of organic matter, riparian broadleaves are likely to mitigate these effects in stream ecosystems. This project illustrates the value of blending catchment-scale studies with field-based mesocosms to understanding complex global change processes.

Global change is expected to accelerate extinction rates substantially. Accurately predicting species responses to future climate and land use changes and the conservation effectiveness of protected areas are critical. Here, I test whether species distribution models can predict how species' ranges shift through time and if protected areas are more robust to recent global change impacts than areas lacking formal protection. Purely spatial species distribution models are able to predict how species' distributions have changed over the 20th century for many species. However, because this predictive ability was not strongly related to biological or sampling characteristics considered here, there is no a priori way to determine which species' models will accurately predict range shifts through time. Protected areas rarely performed differently than randomly selected, unprotected areas in terms of species richness change and species composition change over the past century. Conservation strategies should focus on improving landscape connectivity to facilitate species' geographical responses to future global changes and should account for uncertainty in predictions of those responses.

Evidence suggests that human-driven changes to the earth are having clear and profound effects on many species, as well as the species with which they associate. Disruptions in the interactions between species can change the community structure, in turn changing the dynamics of entire ecosystems. The following dissertation examines how the impacts of climate change related events and invasive species may influence biotic interactions and impact orchid populations and range distributions. Here I quantify how orchid pollinators and mycorrhiza vary between species with different life histories, and between and within habitats. The results showed that orchids with wide range distributions (i.e. geographic or elevational) were more generalized in their mycorrhizal fungi requirements than co-occurring rare and/or narrow ranging species; the rarer species were also more likely to be affected by antagonistic fungal interactions. This dissertation makes a critical contribution to understanding plant and orchid ecology, to assisting ongoing orchid recovery efforts worldwide, and ultimately to developing more comprehensive management plans to mitigate future biodiversity losses.

Climate change can have potentially catastrophic effects upon biodiversity and food web structure and according to the fourth IPCC report, ambient temperatures will rise by between 3.0-5.0 °C over the next century, with already an average increase in global surface temperature of ~0.74°C in the past 100 years. This has known implications in ecology from individuals to ecosystems. The microbial loop consists of small organisms ranging in body size from bacteria (1-15 μm), single-celled eukaryotes (10-1000 μm) and multicellular organisms (250 – 1000 μm) that assimilate dissolved organic carbon into the “classical food web”. ! The principal goal of this thesis was to assess how rising global temperatures might impact the natural microbial assemblages in 20 mesocosms under 2 treatments – 10 warmed (in line with IPCC predictions) and 10 ambient. The abundance and body mass of 4 major microbial loop taxa (desmids, flagellates, heterotrophic protists and meiofauna) were quantified at monthly intervals over a 2-year period. Secondly, in a microcosm experiment, the population dynamics of three pure cultures of ciliates were monitored across a temperature gradient; the rate of population decline under starvation and changes in body size were quantified.! Results showed that (1) rising global temperatures alters the size spectrum in the autotrophic protists, (2) temperature interacts with temporal and spatial gradients, resulting in changes in phenology (3) these changes in phenology are observable at both the community level and the population level within the microbial assemblage of the mesocosms and (4) extinction rates and body mass reduction in experimental microcosms were faster at warmer temperatures and partially support predictions of the metabolic theory of ecology.! The implications of these findings are discussed in terms of (1) continued research into the role that small organisms play in community and ecosystem ecologyand (2) the use of these small organisms in experiments as models to inform ecological theory by scaling up from microcosms and finally, (3) I discuss future directions in freshwater microbial ecology, focusing on the increased use of molecular techniques.

Assessments of the anthropogenic threats to savanna ecosystems are primarily focussed on land use change, bush encroachment, and biological invasions. There is, however, very little understanding as to the threats from atmospheric pollution. South Africa is the major emitter of CO2 on the African content while the Mpumalanga region bordering the Kruger National Park (KNP) is among the leading regions for nitrous oxide pollution in the world. It is not only increasing atmospheric pollution, but rainfall intensity is also predicted to increase for southern Africa. As savannas are nutrient limited, an increase in nitrogen deposition will have major consequences for vegetation structure and this can only be exacerbated by increased rainfall amounts. Current research suggests that these predicted increases in water and nutrients will result in increasing grass biomass and decreasing herbaceous species richness. The effects of global change drivers on savanna vegetation are also likely to propagate through to multiple trophic levels, with changes in vegetation structure cascading down to invertebrate assemblages. As invertebrates are ubiquitous, form the bulk of metazoan species diversity and biomass on earth, and play a pivotal role in many ecosystems, I discuss in the introductory chapter of this thesis why the influence of global change on these assemblages should not be ignored. In my first data chapter, Chapter 2, I examine the effect that increases in available nutrients and water may have on vegetation structure, and how this may cascade down to grasshopper and ant assemblages. I do this using a fully factorial experiment in KNP with nutrient and water additions where I assessed both herbaceous (forb and grass) and insect (ant and grasshopper) assemblages five years after resource additions began. My results show that there was a substantial increase in grass biomass while plant and insect species richness declined with water addition alone and that a combination of nutrients and water resulted in the greatest increases in grass biomass and concomitant decreases in plant and insect species richness. The effects of nutrient and water additions on the insect community assembly was primarily driven by a decrease in grasshopper species and ant abundance respectively. An analysis of ant functional traits showed that the rare ant species mediated the impact of the resource additions on the ant assemblage. Fire is inherent to savanna systems with profound effects on vegetation structure. There has, however, been relatively little research on the effects of fire on savanna invertebrate fauna. In Chapter 3 I look at the effect that fire may have on the vegetation and insect community assembly at my study site between five and eight months after the site had been burned. These results show an increase in grass biomass and decrease in plant and insect species richness with a combination of nutrients and water. My results also show that grasshopper biomass, abundance, and species richness decreased as herbaceous biomass decreased. While ant species richness decreased, ant abundance increased post-fire, primarily related to an increase in patches of bare ground. With global change, drought frequency is also expected to increase. The insect and grass assemblages, both on and off Macrotermes mounds, at two sites in the southern section of KNP had been sampled in a separate study in 2012. In Chapter 4 I describe a study where I resampled these mounds during the peak of the most severe drought in 30 years. The two sites differed in drought severity, one where the drought severity was very high and the other where severity was much lower. The objective was to determine the effects that drought may have on the grass and associated insect assemblages both on and off termite mounds. My results show that at the high severity site grass cover and biomass and grasshopper abundance decreased both on and off mounds. The overall reduction in habitat structure resulted in an increase in both ant abundance and species richness but the mound and matrix ant assemblages diverged during drought. Where the drought was less severe there was an increase in large mammal herbivores as animals moved out of the more affected areas. This increase in mammal herbivory was more evident on rather than off mounds resulting in grass biomass being lower on rather than off mounds. The cascading effect saw grasshopper abundance decrease on and increase off mounds. The mound and matrix ant assemblages did not respond to the comparatively smaller change in habitat structure. Finally, in the synthesis chapter I discuss my results in the broader context of how global change drivers such as increased nitrogen deposition may cascade down from plant to insect community assembly. At present there is very little understanding of the amounts of nitrogen being deposited in KNP or the effect that this may have. The results of my study would suggest that this increase in nitrogen deposition will have major consequences for vegetation structure and that this will cascade down to the insect assemblage. In mitigating for this, it is therefore essential that management in KNP adapt a monitoring protocol for nitrogen deposition, especially when considering that where N deposition is really high fire may not volatilise everything to allow the system to reset itself back to its original state. It is not only nitrogen deposition, but drought frequency is also likely to increase. In mitigation for this there should also be monitoring programmes to consider the effects of drought as animals may move from areas of high drought severity to areas where severity is lower. Such movement will increase grazing pressure on both low and high nutrient environments with cascading effects on vegetation structure and insect assemblages.

Biodiversity rich regions worldwide face threats from various global change agents. This research quantifies environmental influences on vegetation, and the impacts of exotic woody plant invasion and anthropogenic nitrogen (N) deposition in a global biodiversity hotspot. The study was conducted in the montane grasslands of the Nilgiris, Western Ghats, and outlines potential management options for this region. Specifically, I examined (1) the role of environmental factors in influencing native plant distribution and ecosystem properties, (2) the status and impact of exotic shrub (Scotch broom, henceforth broom) invasion, (3) the role of disturbances in the success of broom, (4) the role of fire in restoring invaded grasslands, and (5) the impacts of terrestrial N loading on the grassland ecosystem. I used experiments and surveys to assess these. Distributions of several key species were explained by a few complex environmental gradients. In invaded-grasslands, broom populations consisted mainly of intermediate size and age classes, with no clear indication of population decline. Invasion negatively impacted plant community structure and drastically changed composition, favoring shade-tolerant and weedy species. However, invasion did not greatly alter ecosystem function. Fire successfully eliminated mature broom stands, but resulted in a short-term increase in broom seedling recruitment. At the end of 18 months, the fire effects on uninvaded-grasslands were not apparent, but there was no conclusive evidence of the formerly invaded patches attaining the composition of uninvaded-grasslands following burning. N fertilization strongly influenced soil N dynamics, and shoot N concentrations, but effects on aboveground production were weak. Surprisingly, N enrichment had positive effects on diversity in the short-term. It is clear that these grasslands need immediate management intervention to forestall degradation from invasion. Fire could be used to eliminate mature broom stands and deplete persistent seedbanks, which will facilitate colonization by shade-intolerant grassland plants. Active restoration should be mindful of environmental preferences of framework species. Long-term studies of the impacts of N deposition in the context of disturbances will help determine realistic critical thresholds and utilize disturbances to buffer the potential adverse effects of increasing N loading.

Root biomass, production and decomposition have been poorly studied in peatland ecosystems despite evidence that they may be equal to or greater than aboveground vascular plant components and contribute significantly more carbon (C) to the soil organic matter pool. At the same time, global change phenomena such as water table drawdown (via rising temperatures) and increased nitrogen deposition threaten to dramatically alter these systems, primarily through changes to vegetation. This makes quantifying root biomass, production, and decomposition critical to our understanding of peatland C cycles. Understanding how these belowground stocks and fluxes vary with aboveground plant components in relation to environmental (climate, water table) and biological (vegetation type, species diversity) factors can provide insight into how peatland plant communities adapt to different environments. Additionally, the implications of these adaptations to C cycling within and among sites and in response to global change phenomena can then be considered. My research began by evaluating relationships between above- and belowground biomass and production in a review of wetland plant communities and determining the effect of vegetation type and environmental and climate factors on these relationships within and among wetland types, including peatlands. My subsequent research focused specifically on bog plant communities in Canada and Finland, and the relationships between belowground biomass and production and aboveground biomass as they relate to variations in water table, a key environmental driver within a bog site. Root decomposition rates, a crucial process determining root contributions to soil organic matter, were also studied for the two main evergreen and deciduous shrub species as they relate to root size and soil depth using the litterbag method. The final component of my research evaluated the response of root pro
Peu d'études ont été faites sur la biomasse racinaire, sa production et décomposition malgré les évidences montrant qu'autant la biomasse racinaire et sa production serait égale ou plus grande que la contrepartie hors terre des plantes vasculaires. De plus, celles-ci contribueraient de manière significative à la quantité de C (carbone) contenue dans le réservoir de matière organique du sol. Les changements globaux tels la diminution du niveau de la nappe phréatique (via une augmentation des températures), et une augmentation de la quantité d'azote dans les dépôts atmosphériques menacent de changer de manière dramatique l'équilibre de ces systèmes par un changement de végétation. Mesurer la biomasse racinaire, sa production et décomposition est critique pour notre compréhension du cycle du C dans les tourbières. Une meilleure connaissance des processus régissant la variation des réservoirs et flux souterrains par rapport à la composante hors-terre des plantes, en relation avec les facteurs environnementaux (climat, nappe phréatique) et biologique (type de végétation, diversité des espèces) permet de mieux comprendre comment les communautés de plantes de tourbières s'adaptent aux différents environnements. De plus, nous devons considérer l'implication de ces adaptations dans le cycle du C à l'intérieur d'un site et entre différents sites, et ce en réponse au phénomène de changement globaux. Ma recherche débute par l'évaluation de la relation entre la biomasse et la production souterraine et hors-terre à l'aide d'une revue de littérature des communautés de plantes de milieux humides. Cette revue de littérature permet de déterminer l'effet des types de végétation, de l'environnement et des facteurs climatiques sur les différentes relations à l'intérieur d'un même site et entre différents types de milieux humides, incluant les tourbières. Les recherch

Emiliania huxleyi is a ubiquitous coccolithophore present throughout the global ocean and capable of forming large blooms with significant effects on the global carbon cycle. Developing our understanding of E. huxleyi ecology is necessary in order to better quantify E. huxleyi’s role in the present carbon cycle, and to predict its role in the future carbon cycle under climate change scenarios. Major gaps in the understanding of E. huxleyi ecology were addressed using (1) controlled mesocosm experiments in June 2008 in Raunefjord, Norway, to map population genetics of E. huxleyi blooms in relation to ecological pressures (viruses and rapid growth), (2) biogeographic sampling of nannoplankton (2 - 20 μm) in the SO, including E. huxleyi, to determine ecological pressures on E. huxleyi blooms in situ (environmental gradients), and (3) controlled iron (Fe) addition bioassay experiments in the SO to establish the role of Fe gradients in the nannoplankton community relative to the phytoplankton community. During the mesocosm experiments, 279 individual E. huxleyi cells were isolated to establish clonal cultures, of which 143 were successfully genotyped using 5 microsatellite molecular markers. Both high gene diversity and two distinct genotypic populations were detected over the bloom time series and are strong evidence for a large reservoir of genetic variability within the E. huxleyi species concept, which may translate into phenotypic plasticity, such as differing levels of viral resistance. In the SO, the spatial and temporal biogeography of the three most numerous mineralizing nannoplankton groups, the coccolithophore E. huxleyi, the smaller (<20 μm) species of the diatom genus Fragilariopsis, and chrysophytes of the genus Tetraparma were defined using scanning electron microscopy (SEM) analysis in conjunction with an array of biological, physical, and chemical variables during two successive cruises to the Scotia Sea. Multivariate statistical analyses were used to identify the most influential environmental variables controlling mineralizing nannoplankton biogeography. Sea surface temperature (SST) and salinity were identified as primary variables and removed from the analysis, leaving frontal boundaries, macronutrient, and dFe concentrations significantly associated with a northern E. huxleyi-dominated community (group I; higher nutrients) and a southern Tetraparma- and Fragilariopsisdominated community (group II; lower nutrients). Estimates of biomass indicated that the Scotia Sea mineralizing nannoplankton community formed a substantial part (on average 13%) of the total phytoplankton community. The results of bioassay Fe incubations indicated a response in medium and large diatoms and E. huxleyi, and a number of microplankton (20 – 200 μm) diatom species. Overall, the work contributes substantially to our understanding of the molecular population structure, extent of phenotypic plasticity, and environmental parameters affecting the key phytoplankton E. huxleyi.

Global climate change is increasing the number of hot days along the California coast as well as increasing the incidence of off-shore upwelling events that lower the pH of intertidal seawater; thus, intertidal organisms are experiencing an increase in more than one stress simultaneously. This study seeks to characterize the global protein response of the eurythermal porcelain crab Petrolisthes cinctipes to changes in thermal, pH, and tidal regime treatments, either combined or individually. The first experiment examined temperature stress alone and sought to determine the effect of chronic temperature acclimation on the acute heat shock response. We compared the proteomic response of cheliped muscle tissue following a month-long acclimation to either (1) constant 10°C, (2) daily fluctuation from 10-20°C, or (3) daily fluctuation from 10-30°C, all followed by either a 30°C acute heat shock or 10°C control. We found that ATP supply via the phosphagen system, changes in glycolytic enzymes, muscle fiber restructuring, respiratory protein fragmentation, and immunity were primarily affected by acclimation and subsequent heat shock. Acclimation to the “extreme” regimes (10°C and 10-30°C) resulted in the greatest proteomic changes, while acclimation to the moderate regime (10-20°C) resulted in a more mild response to heat shock (i.e., fewer adjustments to relative protein abundance). The second experiment sought to determine the proteomic response of gill tissue following a 17 d acclimation to daily changes in pH (ambient pH 8.1 vs low pH 7.6), tidal regime (constant immersion vs 6 h emersion), and temperature (ambient 11°C vs 22-31°C heat shock during emersion). Low pH alone reduced expression of molecular chaperones of the endoplasmic reticulum, lectins, and serine proteases involved in activating the prophenoloxidase cascade. It also increased the abundance of Na+/K+-ATPase, nitrogen metabolism enzymes, and induced changes in tubulin expression, all suggesting an increase in ammonium excretion. Addition of emersion during low pH reduced the abundance of several metabolic proteins including those involved in the proposed ammonium excretion mechanism, suggesting a decrease in metabolic function in part to prevent toxic accumulation of ammonium in the branchial chambers. Combined pH, emersion, and thermal stress increased the abundance of proteins involved in cuticle binding and crosslinking. These results indicate that the responses to pH, tidal cycle, and temperature are highly dependent on one another and that changes in ER protein maturation, ion transport, immunity, and cuticle structure are the primary biochemical systems impacted by these environmental stressors in crustacean gill.

The Chocó-Darien Global Ecoregion (CGE) in South America is one of 25 global biodiversity hotspots prioritized for conservation. I performed the first land-use and land-cover (LULC) change analysis for the entire CGE in this dissertation. There were three main objectives: 1) Select the best available imagery to build annual land-use and land-cover maps from 2001 to 2015 across the CGE. 2) Model LULC across the CGE to assess forest change trends from 2002 to 2015 and identify the effect of proximate causes of deforestation and reforestation. 3) Estimate the effects of underlying drivers on deforestation and reforestation across the CGE between 2002 and 2015. I developed annual LULC maps across the CGE from 2002 to 2015 using MODIS (Moderate Resolution Imaging Spectro radiometer) vegetation index products and random forest classification. The LULC maps resulted in high accuracies (Kappa = 0.87; SD = 0.008). We detected a gradual replacement of forested areas with agriculture and secondary vegetation (agriculture reverting to early regeneration of natural vegetation) across the CGE. Forest loss was higher between 2010-2015 when compared to 2002-2010. LULC change trends, proximate causes, and reforestation transitions varied according to administrative authority (countries: PanamanianCGE, Colombian CGE, and Ecuadorian CGE). Population growth and road density were underlying drivers of deforestation. Armed conflicts, Gross Domestic Product, and average annual rain were proximate causes and underlying drivers related reforestation.

Methane hydrates are frozen deposits of methane and water found in high pressure or low temperature sediments. When these deposits destabilize, large quantities of methane can be emitted into the atmosphere. This is significant to climate change because methane has 25 times more greenhouse gas potential than Carbon Dioxide. Worldwide, it is estimated there are between 2500 and 10000 gigatons of methane stored in hydrate deposits. This represents more carbon than all fossil fuels on Earth. It is estimated that between 200 and 2000 gigatons of methane are stored in hydrates in Arctic waters acutely vulnerable to greenhouse warming. Over the last decade, researchers have identified instances of hydrate destabilization that have already begun. To gain insight into the potential climatic effects widespread hydrate dissociation would have, researchers have examined hydrate dissociation during the Paleocene Eocene Thermal Maximum 55 million years ago as a geologic precedent. In this period, large-scale hydrate dissociation contributed to 5-8 degree Celsius warming worldwide. If such a climatic shift were to transpire today, impacts on society would be enormous. There is currently a debate in the scientific community as to whether the risk of methane hydrate dissociation is relevant to the present generation. One side argues that not enough methane could be emitted into the atmosphere from today’s hydrate sources to have a meaningful impact on climate warming, where the other side contends that more than enough methane could be emitted from present day hydrate deposits to cause significant impacts to the global greenhouse effect. Given the information currently known about hydrates, it is reasonable to conclude there is a moderate risk of widespread destabilization that could impact global climate change in the coming decades. Significant acceleration of the conversion to alternative energies and implementation of geoengineering strategies should be considered.

La conservación requiere una previsión estratégica que permita abordar con eficacia los retos actuales que plantea el cambio global. La cuenca Mediterránea ha sido identificada como área prioritaria para la conservación, particularmente vulnerable al efecto combinado del cambio climático, el cambio de los usos del suelo y el régimen de perturbaciones por incendios forestales. Los efectos de la interacción de estos factores de cambio y las grandes incertidumbres asociadas a su predicción, también pueden ser vistas como una oportunidad para intervenir a través de mejores políticas de conservación. Los ejercicios de previsión estratégica pueden ofrecer a los responsables de la toma de decisiones herramientas para pensar de forma creativa y proactiva sobre el futuro y tomar decisiones que creen un futuro más deseable. En esta tesis ilustramos el papel de las actividades de ‘horizon scanning’, planificación y análisis de escenarios basados en simulación, en los que se sustenta el enfoque de previsión estratégica, y en la que usamos escenarios conceptuales como líneas argumentales y simulaciones como estimaciones numéricas de los futuros cambios ambientales. En particular, este ejercicio de previsión estratégica contribuye a la apertura de dos opciones de políticas de manejo del fuego prometedoras ('dejar quemar los incendios no planificados' y 'la extracción de biomasa forestal para bioenergía') alternativas al paradigma actual de 'apagar todos los incendios'. Ambas políticas de manejo del fuego podrían combinarse estratégicamente con el fin de alcanzar los objetivos de reducción de combustible requeridas para mitigar el creciente impacto de los grandes incendios causados por el cambio global. La planificación de la conservación puede ser mejorada considerablemente mediante la aplicación de estas estrategias de manejo del fuego. Dos principales oportunidades de conservación emergentes han sido identificados y deben ser priorizadas a fin de proteger de forma efectiva las especies de aves de interés comunitario en un futuro próximo: 1) la creación de etapas tempranas de sucesión de la vegetación para especies de hábitat abierto a través de políticas de 'dejar quemar incendios no planificados'; y 2) el aumento de la capacidad de resiliencia frente al cambio climático de los hábitats forestales claves para las especies más forestales. En esta tesis se hace hincapié en la necesidad de una perspectiva de conservación integral en donde las políticas agrícolas, forestales y de manejo de fuego deben ser consideradas explícitamente para preservar eficazmente hábitats clave para las aves más amenazadas en sistemas altamente dinámicos propensos al fuego. Nuestros resultados también arrojan luz sobre la importancia de considerar la dinámica del paisaje y las sinergias entre las diferentes fuerzas motrices a la hora de evaluar a largo plazo la eficacia de la gestión del fuego en la reducción del riesgo de incendios y la protección de la biodiversidad en los ecosistemas de tipo mediterráneo.
Conservation needs strategic foresight leading to effectively address the ongoing challenges posed by global change. Mediterranean Basin has been identified as priority area for conservation, particularly vulnerable to the combined effects of climate change, land-use change and fire disturbance regime. The interacting effects of these drivers, and the large uncertainties associated to their forecasting, might also bring conservation opportunities to intervene through better policies. Strategic foresight exercises may offer decision-makers with tools to creatively think about the future and make decisions that create a more desirable future. In this thesis, we illustrate the role for horizon scanning, scenario planning and simulation-based scenario analysis in underpinning the strategic foresight approach — using storylines as conceptual scenarios, and simulations as numerical estimates of future environmental changes. In particular, this strategic foresight exercise contributes to opening up two promising fire management policy options (‘letting unplanned fires burn’ and ‘forest biomass extraction for bioenergy uses’) alternatives to the current fire suppression paradigm of “stopping all fires’’. Both fire management policies could be strategically combined in order to achieve the fuel reduction objectives required to mitigate the increasing impact of large fires caused by global change. Conservation planning may be considerably improved through the implementation of such fire management strategies. Two main emerging conservation opportunities have been identified and should be prioritized in order to effectively protect community-interest bird species in the near future: 1) promoting early-succession stages of vegetation for open-habitat dwelling species through ‘letting unplanned fires burn’ policies; and 2) increasing the resilience of key forest habitats to climate change for forest-dwelling species. This thesis emphasizes the need for an integrative conservation perspective wherein agricultural, forest and fire management policies should be explicitly considered to effectively preserve key habitats for threatened birds in fire-prone, highly-dynamic systems. Our findings also shed light about the importance of considering landscape dynamics and the synergies between different driving forces when assessing the long-term effectiveness of fire management at reducing fire risk and safeguarding biodiversity in Mediterranean-type ecosystems.

The presence of annual density banding in certain long-lived reef-building corals provides a record of the coral’s growth rate over time in response to changing environmental conditions. Coral growth is best described by three parameters: linear extension, bulk density, and calcification. Coral growth is generally controlled by the combined influences of light, temperature, and water quality; however, corals are highly responsive to their surrounding conditions and thus record environmental variations through their rates and patterns of skeletal accretion. Because coral growth rates reflect environmental conditions over time, they allow testing of hypotheses regarding the effects of climate change, more specifically global warming which affects sea surface temperatures and rising atmospheric carbon dioxide which affect the aragonite saturation state of seawater. Influences on coral growth include local changes in sea surface temperature and rainfall as well as large scale climatic indices such as the Atlantic Multidecadal Oscillation (AMO), the North Atlantic Oscillation (NAO), and the Southern Oscillation Index (SOI). Chapter 1, Background, reviews the current state of knowledge in three primary areas: 1) coral biology, growth, density band formation, and measurement of extension, density, and calcification, 2) potential climate change impacts on coral growth, and 3) long-term coral growth records. This section is broadly intended to review the literature, identify possible information gaps, and recognize current debate within coral and climate change research. Chapter 2, Sample Size for Coral Sclerochronology, presents data of sample size correlations based on statistical analyses of annual extension rates. A standardized period (1970-1985) of annual extension rates from the largest number of Montastraea faveolata samples available from southeast Florida (136 corals) was used to test correlation on varying spatial scales and to determine sample size requirements for desired levels of correlation based on objective criteria. The results provide basic information on masterchronology construction for sclerochronological growth rate studies and provide a framework from which further growth rate variability can be assessed. Extension and bulk density can be measured from X-ray films of coral skeletal slabs and can be used to calculate calcification. Chapter 3, Relative Optical Densitometry, describes the techniques and associated errors through the process of coral coring, sectioning, X-raying, developing, digitizing, calibrating and analyzing. The principles of relative optical densitometry and the calculation of mass absorption coefficient ratios for aragonite and aluminum standards are explained. Calculated and measured errors are quantified to define the accuracy and precision of these techniques necessary to detect potentially subtle changes in coral growth caused by climate change. Coral cores from the Florida Key, USA, were used to construct growth records over a 60-yr period from 1973-1996. Chapter 4, Coral Growth Records and Climate Change, uses linear extension rate, bulk-density, and calcification rate from annual and sub-annual bands in order to assess: 1) growth averages, variability, and relationships between growth parameters, 2) long term trends with respect to rising carbon dioxide levels and sea surface temperature, 3) correlation with local environmental variables of temperature and rainfall, and 4) correlation with major climate indices of Atlantic Multidecadal Oscillation, North Atlantic Oscillation, and the Southern Oscillation.

Amazonian evergreen forests are of broad interest, attributable to their ecological, economic, aesthetic, and cultural importance. However, their fate under climate change remains uncertain, largely due to unclear mechanisms in regulating tropical photosynthetic metabolism. Understanding mechanistic controls on these dynamics across time scales (e.g. hours to years) is essential and a prerequisite for realistically predicting tropical forest responses to inter-annual and longer-term climate variation and change. Tropical forest photosynthesis can be conceptualized as being driven by two interacting causes: variation due to changes in environmental drivers (e.g. solar radiation, diffuse light fraction, and vapor pressure deficit) interacting with model parameters that govern photosynthetic behavior, and variation in photosynthetic capacity (PC) due to changes in the parameters themselves. In this thesis, I aim to reveal photosynthetic controls by addressing three fundamental but complementary questions: (1) What are the mechanisms by which the subtle tropical phenology exert controls on tropical photosynthetic seasonality? (2) How do the extrinsic and intrinsic controls regulate the photosynthesis processes at hourly to interannual time scales in an Amazonian evergreen forest? (3) Are there sufficiently consistent relations among leaf traits, ages, and spectra that allow a single model predict the leaf aging process of Amazonian evergreen trees? To address question 1, I firstly show that seasonal change in ecosystem-scale photosynthetic capacity (PC), rather than environmental drivers, is the primary driver of seasonality of gross primary productivity (GPP) at four Amazonian evergreen forests spanning gradients in rainfall seasonality, forest composition, and flux seasonality. Using novel near-surface camera-detected leaf phenology to drive a simple leaf-cohort canopy model at two of these sites, I further show that leaf ontogeny and demography explain the changes in ecosystem photosynthetic capacity. The coordination of new leaf growth and old leaf divestment (litterfall) during the dry season shifts canopy composition towards younger leaves with higher photosynthetic capacity, driving large seasonal increases (~27%) in ecosystem photosynthetic capacity. To address question 2, I used the 7-year eddy covariance (EC) measurements in an Amazonian tropical evergreen forest. I used a statistical model to partition the variability of 7-year EC-derived GPP into two main causes: variation due to changes in extrinsic environmental drivers and variation in intrinsic PC. The fitted model well predicts variability in EC-derived GPP at hourly (R²=0.71) to interannaul (R²=0.81) timescales. Attributing model predictions to causal factors at different timescales, I find that ~92% of the variability in modeled hourly GPP could be attributed to environmental driver variability, and ~5% to variability in PC. When aggregating the modeled GPP into the annual time-step, the attribution is reversed (only ~4% to environment and ~91% to PC). These results challenge conventional approaches for modeling evergreen forests, which neglect intrinsic controls on PC and assume that the primary photosynthetic control at both long and short timescales is due to changes in the hourly-to-diurnal environment on the physiological phenotype. This work thus highlights the importance of accounting for differential regulation of different components of GPP at different timescales, and of identifying the underlying feedbacks and adaptive mechanisms which regulate them. To address question 3, I explored the potential for a general spectrally based leaf age model across tropical sites and within the vertical canopy profiles using a phenological dataset of 1831 leaves collected at two lowland Amazonian forests in Peru (12 species) and Brazil (11 species). This work shows that a simple model (parameterized using only Peruvian canopy leaves) successfully predicts ages of canopy leaves from both Peru (R²=0.83) and Brazil (R²=0.77), but ages for Brazilian understory leaves with significantly different growth environment and leaf trait values have lower prediction accuracy (R²=0.48). Prediction accuracy for all Brazilian samples is improved when information on growth environment and leaf traits were added into the model (5% R² increase; R²=0.69), or when leaves from the full range of trait values are used to parameterize the model (15% R² increase; R²=0.79). This work shows that fundamental ecophysiological rules constrain leaf traits and spectra to develop consistently across species and growth environment, providing a basis for a general model associating leaf age with spectra in tropical forests. In sum, in this thesis, I (1) conceptualize photosynthesis as being driven by two interacting dynamics, extrinsic and intrinsic, (2) propose and validate a model for biological mechanisms that mediate seasonal dynamics of tropical forest photosynthesis, (3) assess and quantify the factors controlling tropical forest photosynthesis on timescales from hourly to interannual, and (4) develop a general model for monitoring leaf aging processes of tropical trees across sites and growth environments. The revealed mechanisms (and proposed models) in this thesis greatly improve our mechanistic understanding of the photosynthetic and phenological processes in tropical evergreen forests. Strategic incorporation of these mechanisms will improve ecological, evolutionary and earth system theories describing tropical forests structure and function, allowing more accurate representation of forest dynamics and feedbacks to climate in earth system models.

Drought-induced tree mortality is an emerging global phenomenon that appears related to climate change and rising temperatures in particular, and may be an early indication of vegetation change. However, vegetation response to climate change is uncertain, particularly for future novel climates. Notably, no current models of vegetation change attempt to mechanistically predict plant mortality, and in particular, mortality of trees, which exerts strong influences on ecological function. Resolving uncertainties surrounding the physiological mechanism and temperatures sensitivity of tree mortality is a current challenge in global change ecology. The objectives of this dissertation were to 1) consider tree mortality consequences for earth system processes related to carbon, water, and energy exchange that include climate regulation; 2) explore tree mortality effects on the water cycle by developing hypotheses and research needs; 3) quantify the temperature sensitivity of drought-induced tree mortality and gain insight into the physiological mechanism of mortality; 4) quantify the relationships among temperature, stored carbohydrate resources, and gas exchange to further elucidate physiological tree mortality mechanisms; and 5) quantify the sensitivity of two species of pine seedlings to progressively elevated temperatures and relate mortality to the effect of temperature on carbon metabolism. Major findings of this dissertation relate to the temperature sensitivity, physiological mechanism, and implications of tree mortality. Assessment of the potential consequences of tree mortality for earth system processes documented the contrasting influences of tree mortality on the terrestrial C cycle and land-surface energy exchange, the balance of which will determine the net effects on climate regulation (Appendix A). Following a survey of the ecohydrology literature, thresholds for tree mortality to cause watershed changes were hypothesized at ~20% loss of canopy cover, ~500 mm of annual precipitation, and whether flows are snowmelt dominated (Appendix B). Elevated temperature (~+4°C) accelerated tree mortality by 28% during experimental drought, a difference related to cumulative respiration dynamics in piñon pine (Appendix C). Stored carbohydrate resources were declined during lethal drought but were not entirely depleted prior to mortality (Appendix D). Seedlings exhibited progressive declines in time-to mortality with increased temperatures, a response related to C metabolism (Appendix E).

Picophytoplanktonic cells (0.2-2 μm) are the dominant phytoplankters in the largest marine biomes on Earth: the subtropical gyres. The overaching aim of this thesis is to develop algorithms that use remote-sensing observables to map the distribution of the smallest and most abundant member of picophytoplankton, Prochlorococcus, and assess its contribution to the marine carbon cycle. To understand how the photoacclimatory status and growth of Prochlorococcus and its sister genera Synechococcus are influenced by light and nutrients, experiments were conducted in the South Atlantic Gyre (SAG). Results from the manipulation experiments show that, in the central region of the SAG, nutrient addition can induce marked changes in the optical properties of Prochlorococcus cells when subjected to saturating light levels, leading to a decrease in cell abundance, whereas at the gyre periphery no substantive changes in cell growth or optical characteristics were observed. Since light plays a central role in shaping the distribution of cyanobacteria, an empirical algorithm based on relationships between Prochlorococcus abundance and remotely-sensed observables was developed. The outputs were then used in a modified primary production model to predict the vertical distribution of carbon fixation by Prochlorococcus. The models estimate that ∼ 3.4 x 10²⁷ Prochlorococcus cells in the global ocean fix 4.7 Gt C year^-1. Most of the cell biomass and primary productivity is concentrated in the subtropical gyres and areas near the Equatorial Convergence, and 61% of the carbon fixation occurs in the upper water column (0-45 metres), where only 43% of the cells reside. However, in the gyres, carbon fixation is highest (62%) in deeper layers (45-200m), and both cell abundance and carbon fixation show marked seasonal patterns. The models developed in this study provide an unprecedented view of the vertical distribution of Prochlorococcus cells and their corresponding rates of carbon fixation in the global ocean.

In alpine regions worldwide, climate change is dramatically altering ecosystems, affecting biodiversity across habitats and taxonomic scales. For streams, the associated recession of mountain glaciers and snowfields, paired with altered precipitation regimes, are driving shifts in hydrology, species distributions, and basal resources – often threatening the very existence of some habitats and biota. Globally, alpine streams harbor particularly substantial species and genetic diversity due to significant habitat insularity and environmental heterogeneity: however, anthropogenic warming threatens to homogenize habitats through the reduction of the cryosphere, thereby reducing biodiversity from micro- to macroscopic organisms and genes to communities. Still, alpine stream biodiversity, particularly in North America, is poorly understood, making it difficult to predict future changes without baselines for comparison. For my dissertation, I used genetic tools to assess biodiversity in alpine streams of the central Rocky Mountains in North America. Here, I begin by reviewing the current state of alpine stream biology from an organismal perspective. Next, I provide two perspectives on macroinvertebrate diversity. The first, a population genetic comparison of three highly similar species, is followed by a fine-scale genomic study of one species, Lednia tumana. I follow these largely macroinvertebrate-centric chapters with a modern synthesis of the microbial ecology of mountain glacier ecosystems. Finally, I conclude with a study of microbial diversity that addresses how microbial diversity is shaped by geography, habitat, and hydrological source in North America. Collectively, this research refines existing themes in alpine stream biology by revealing unexpected differences in population genetic patterns among closely related species, the influence of recent deglaciation on population genetic structure and demographic history of a threatened stonefly, and clarification of the environmental drivers shaping microbial diversity.

Lichens are, in most cases, sensitive to anthropogenic factors such as air pollution, global warming, forestry and fragmentation. Two studies are included in this thesis. The first is an evaluation of the importance of old oak for the rare epiphytic lichen Cliostomum corrugatum (Ach.) Fr. This study analysed whether C. corrugatum was limited by dispersal or restricted to tree stands with an unbroken continuity or the substrate old oaks. The results provide evidence that the investigated five populations in Östergötland, Sweden, of C. corrugatum exhibit substantial gene flow, an effective dispersal and a small genetic variation between the sites. Most of the genetic variation was within the populations. Thus, C. corrugatum is more dependent of the substrate old oaks, rather than limited by dispersal. The second study investigated possible range shift of some common macrolichens, due to global warming, from 64 sites in southern Sweden comparing the two years 1986 and 2003. The centroid of three lichen species had moved a significant distance, all in a north east direction: Hypogymnia physodes (L.) Nyl. and Vulpicida pinastri (Scop.) J.-E. Mattsson and M. J. Lai on the tree species Juniperus communis L. (50 and 151 km, respectively) and H. physodes on Pinus sylvestris L. (41 km). Considering also the non-significant cases, there is strong evidence for a prevailing NE direction of centroid movement.

Traditionally, hypoxia has been defined as the situation where DO levels have fallen below 2.0 mg O2 L-1, but increasing evidence suggests that this low level of DO is inadequate to describe the onset of hypoxia impacts for many organisms. Consequently, there is a need for a greater understanding of how ‘moderate’ alterations in DO levels will affect ecosystem processes and functionality, specifically through behavioural and physiological alterations at the organism and community level. This thesis reports on mesocosm experiments which were conducted to examine the effects of moderate ( > 3.0 mg O2 L-1) hypoxia on firstly, a key ecosystem engineer, the brittlestar Amphiura filiformis, and secondly, on the Station L4 infaunal macrobenthic community. Station L4 is a longstanding marine biodiversity and MSFD reference site and forms part of the Western Channel Observatory. At the organism level, short-term (14 d) exposure to moderate hypoxia significantly reduced oxygen uptake rates, oocyte diameter and oocyte development in A. filiformis. However, these physiological affects occurred irrespective of brittlestar population density. Additionally, moderate hypoxia reduced brittlestar activity, in terms of bioturbation behaviour, consequentially having an effect on ammonium and silicate fluxes. These observations were only detected when brittlestar population density was high. It was concluded that denser populations of A. filiformis may therefore exhibit the greatest changes in behaviour and shifts in ecosystem function as competition for resources and oxygen heightens. The benthic community at Station L4, displayed considerable tolerance to medium-term (6 wk.) exposure to moderate hypoxia, in terms of structure, diversity and bioturbatory behaviour, but these results may be different if exposure was longer or more severe. Alterations in nutrient fluxes were detected, but there was little evidence to suggest these changes were due to macrofaunal behavioural alterations. Additionally, results from this study revealed that bringing complex natural communities into the mesocosm caused a substantial loss of individuals and species, mainly due to translocation and disturbance effects. This important insight into the effects of bringing community assemblages into the mesocosm confirms that even with a loss of diversity, the L4 community maintained functionality and was resilient to alterations in DO. This suggests that the L4 benthic community does not depend on any one specific species for the provision of important ecosystem processes, resulting in considerable functional resilience within the L4 system. However, vulnerability to benthic systems may increase if functionality is dominated by species such as A. filiformis. Consequently, moderate hypoxia may not immediately affect benthic communities in terms of structure and diversity, but the physiological effects on individuals, especially to reproductive development, may cause alterations in the quality and quantity of planktonic propagules supplied by benthic species to the pelagic environment. This could affect benthic community diversity and functionality in the long term if repeated hypoxic events occur.

The demise of reef-building corals potentially lies on the horizon, given ongoing climate change amid other anthropogenic environmental stressors. If corals cannot acclimatize or adapt to changing conditions, dramatic declines in the extent and health of the living reefs are expected within the next half century. The primary and proximal global threat to corals is climate change. Reef-building corals are dependent upon a nutritional symbiosis with photosynthetic dinoflagellates belonging to the group Symbiodinium. The symbiosis between the cnidarian host and algal partner is a stress-sensitive relationship; temperatures just 1°C above normal thermal maxima can result in the breakdown of the symbiosis, resulting in coral bleaching (the loss of Symbiodinium and/or associated photopigments) and ultimately, colony death. As ocean temperatures continue to rise, corals will either acclimatize or adapt to changing conditions, or will perish. By experimentally preconditioning the coral Acropora millepora via sublethal heat treatment, the coral acquired thermal tolerance, resisting bleaching during subsequent hyperthermal stress. The complex nature of the coral holobiont translates to multiple possible explanations for acclimatization: acquired thermal tolerance could potentially originate from the host itself, the Symbiodinium, or from the bacterial community associated with the coral. By examining the type of in hospite Symbiodinium and the bacterial community prior acclimation and after thermal challenge, it is shown that short-term acclimatization is not due to a distinct change in the dinoflagellate or prokaryote community. Though the microbial partnerships remain without considerable flux in preconditioned corals, the host transcriptome is dynamic. One dominant pattern was the apparent tuning of gene expression observed between preconditioned and non-preconditioned treatments, showing a modulated transcriptomic response to stress. Additionally several genes were upregulated in association with thermal tolerance, including antiapoptotic genes, lectins, and oxidative stress response genes. Upstream of two of these thermal tolerance genes, inhibitor of NFκB and mannose-binding lectin, DNA polymorphisms were identified which vary significantly between the northern and southern Great Barrier Reef. The impact of these mutations in putative promoter regions remains to be seen, but variation across thermally-disparate geography serves to generate hypotheses regarding the role of regulatory element evolution in a coral adaptation context.

Marine ecosystems are declining worldwide threatened by an increasing number of stressors. Global change-related disturbances have highlighted the need of new complementary conservation measures; for which the knowledge on the affected species, communities and impacts is essential. The species objective of this PhD, the Mediterranean endemic coral Cladocora caespitosa, may serve as a case study of those species that even if seriously threatened, are lacking essential information on key ecological processes and the responses to the rapid environmental changes that are happening globally. Cladocora caespitosa is the only colonial and zooxanthellate scleractinian coral endemic to the Mediterranean Sea. It is a long-lived and ecosystem engineer species, being one of the rare examples of this type of organisms found in shallow Mediterranean communities, and constitutes an invaluable natural patrimony due to its extensive ancient history, its sizeable long-lasting structures and its fragility in the actual context of climate change. Currently, large C. caespitosa bioconstructions are scarce and only a few examples are known, i.e., in Mjlet National Park (Adriatic) or in the Columbretes Islands (NW Mediterranean). The extensive field of colonies and reefs found in the Illa Grossa Bay (Columbretes Islands Marine Reserve) shows a cumulative colony cover of 2900 m2. This population displays a high degree of geographical isolation and its spatial distribution in the bay is highly aggregated. Our results showed that Cladocora caespitosa is a slow growing species (~ 2.5 mm yr-1), with low recruitment and natural mortality rates (~ 0.30 recruits m-2 yr-1 and 1 %, respectively). Strikingly, the obtained results on the reproductive traits of this species differed significantly between Western Mediterranean and Adriatic Seas. Cladocora caespitosa is gonochoric in W Mediterranean, showing a water temperature-associated gonadal cycle that culminates at the end of the summer in contrast to the findings in the Adriatic, where the coral has described as hermaphroditic with the spawning occurring at the beginning of the summer. Global change is rapidly altering Mediterranean marine habitats, primarily through warming and the invasion of new species. The C. caespitosa population in the Illa Grossa Bay suffered mortalities after 9 summers, separated into 2 mortality periods (2003 - 2006 and 2008 - 2012). The highest necrosis rates were recorded during the first mortality period, after the exceptionally hot summer of 2003. Over 50 % of the area covered by C. caespitosa has suffered necrosis after these recurrent mortalities, which were significantly related to warming (summer warming trend: 0.06 °C yr-1). The differences in necrosis found after summers with similar thermal anomalies pointed out to the existence of other acting factors probably related to the interannual temperature context and delayed stress after extreme summers. These results show that while Cladocora caespitosa displays great ecological plasticity, mostly in relation to changing light conditions, it is not adapted to endure the extreme changes in temperature driven by climate change, the most worrying threat for this coral. Regarding to the impact of invasive species, the invasive algae Lophocladia lallemandii and Caulerpa racemosa successfully spread over the Illa Grossa Bay from 2006 to 2012 and overlapped their distribution in the bay with that of C. caespitosa. No lethal effects of the invasive algae were detected on the coral colonies, which showed toxic activity. This may explain the low overgrowth of living colony parts by C. racemosa and the ability of this coral to compete in an algal dominated community. In long-lived corals such as C. caespitosa, recovery from mortalities relies mostly on recruitment, but there are two main obstacles that may compromise recovery. Firstly, the high frequency of mortalities detected during the last decade probably exceed the recovery potential of the low recruitment rates. Secondly, both warming and invasive algae may have delayed and synergetic effects on reproduction, recruitment and juvenile survival. All the results obtained highlight the endangerment of this species facing rapid environmental changes. Cladocora caespitosa is listed in the IUCN Red List of Threatened Species as Data Deficient. However, the information obtained in this PhD points out that this species could meet the criteria to fall into a threatened category.
Los ecosistemas marinos están siendo amenazados por un número cada vez mayor de impactos. Entre ellos, los impactos derivados del cambio global han puesto en relieve la necesidad de nuevas medidas de conservación que puedan complementar a las ya existentes. Sin embargo, su desarrollo requiere un amplio conocimiento de las especies, procesos e impactos implicados. Esta tesis se centra en la adquisición de nuevos conocimientos sobre la biología y ecología del coral Cladocora caespitosa con el objetivo de aportar información útil para su conservación. Los resultados obtenidos pueden servir como guía para la conservación de aquellas especies de características parecidas sobre las que no se tiene información. Esta especie es el único coral escleractinio, colonial y zooxantelado, endémico del Mediterráneo. Se trata de uno de los pocos ejemplos de especie longeva y estructural que se encuentra en las comunidades mediterráneas de aguas someras. La Bahía de L'Illa Grossa (Reserva Marina de las Islas Columbretes) alberga una de las poblaciones más importantes de este coral, con campos de colonias y arrecifes que muestran una superficie acumulada de 2900 m2. Los resultados obtenidos muestran que se trata de una especie de lento crecimiento, con bajas tasas de reclutamiento y mortalidad natural y que muestra importantes divergencias en sus características reproductoras entre el Mediterráneo Occidental y el Adriático. La población de C. caespitosa de Columbretes ha sufrido mortalidades recurrentes asociadas al calentamiento del agua, que han afectado a más de la mitad del área ocupada por este coral en la bahía. Por otra parte, las algas invasoras Lophocladia lallemandii y Caulerpa racemosa han invadido exitosamente la Bahía de L'Illa Grossa, solapando su distribución con la de C. caespitosa. De momento, no se han observado efectos letales de estas algas sobre el coral; sin embargo, se ha podido detectar la actividad tóxica de esta especie, que podría explicar el bajo recubrimiento de sus partes vivas por C. racemosa. El potencial de recuperación de esta población es limitado dado el bajo reclutamiento y la frecuencia e intensidad de las mortalidades. Además, no se pueden descartar efectos subletales del calentamiento y las algas invasoras sobre la reproducción, reclutamiento y la supervivencia de los juveniles. A partir de esta información esta especie podría cumplir los criterios de la UICN para ser catalogada como especie en peligro.

Coastal vegetation plays an essential role in regulating climate change and water quality, especially seagrass meadows that cover up to 1.6 million km2 worldwide and have been identified as globally significant filters and sinks of biogeochemical elements, including organic carbon (Corg) and nitrogen (N). Over the past century, the accelerating intensity and duration of natural and anthropogenic disturbances have caused severe regime shifts in estuarine and other coastal ecosystem dynamics, causing seagrass losses and affecting their valuable ecosystem services. Although knowledge of long-term seagrass ecosystem dynamics is scarce, including baseline conditions prior to major disturbances, the limited available information has been crucial to assess the human and natural impacts on coastal ecosystems. For example, it has provided answers to key questions related to seagrass ecology, climate change and management, including the estimation of the ecological significance and the monetary value of the biogeochemical sinks associated with seagrass ecosystems. This thesis aims to use seagrasses biogeochemical sinks as archives to reconstruct and understand : 1) baseline conditions in Australian estuaries, and spatial variability in environmental change, including perturbations that triggered the loss of seagrass meadows; 2) the significance of seagrasses in global biogeochemical cycles, as well as the processes that support their potential to store carbon and nitrogen; and 3) the losses of biogeochemical sinks as a consequence of seagrass meadow losses through human-induced impacts and extreme weather events. In this thesis, seagrass sedimentary archives encompassing the last few centuries have been studied to decipher centennial-scale environmental change in temperate estuaries, and the impacts of marine heatwaves and eutrophication on the Corg and N biogeochemical sinks associated to seagrass ecosystems. In the first data chapter of this thesis, the impact of land-use change on the ecological dynamics of temperate estuaries in Australia is reconstructed based on the analysis of multiple proxies in seagrass sedimentary archives encompassing the last 500 years. This palaeoecological study revealed the effect of land-use change following European settlement in the 1800s in Australia on the ecological condition of the estuaries, highlighting the deterioration of seagrass meadows following increased coastal development and agriculture activity after World War II. The second data chapter examined the effect of seagrass loss due to eutrophication on seagrass soil Corg stocks and fluxes, and provides pioneering estimates of CO2 emissions following disturbance of seagrass ecosystems that can be used to support the development of seagrass blue carbon projects (conservation and restoration) to mitigate climate change. The results showed that seagrass loss alone does not necessarily drive erosion of soil Corg, but when combined with sufficient hydrodynamic energy at the sediment surface ( > 0.20 m/s in this case), significant losses occurred (88–95% of soil Corg stocks). The study provided first-order estimates of potential CO2 emissions from eutrophication-induced seagrass loss since the 1950s in Australia, with 161,150 hectares of seagrass habitat loss that likely resulted in the release of 11–21 Tg CO2 (equivalent to a 2% increase in annual CO2 emissions from land-use change). These data will be crucial to inform the implementation of seagrass blue carbon into the Australian climate change mitigation policy. The third data chapter assessed soil N stocks and accumulation rates in Australian seagrass meadows, and provides pioneering estimates of soil N depletion following disturbance of seagrass meadows due to eutrophication and marine heatwaves, and identified the main drivers and potential ecological consequences of those losses. The results showed that Australian seagrasses capture 216–910 Gg N yr-1, equivalent to 96–105% of N runoff from Australian catchments. On the other hand, Australian-wide seagrass losses since the 1950s likely resulted in the loss of 435-720 Gg N from their soils, which likely enhanced eutrophication processes and resulted in adverse ecological consequences. This thesis provides novel and key information on the role of seagrasses as biogeochemical sinks and sources. This information can inform management practices of estuarine and other coastal ecosystems and highlights the value of seagrass sedimentary archives for determining baseline cycles and to reconstruct the time-course of ecological change in response to natural and anthropogenic disturbances. This thesis also highlights the need to conserve and restore seagrass meadows due to their value as natural archives and biogeochemical sinks, demonstrating their potential as a Natural-based Solution for contributing to climate change mitigation.

Les aires de répartition des arbres pourraient être grandement affectées par le changement climatique. Les résultats d'analyses paléogéographiques ont montré que ces dernières se sont déjà déplacées avec les variations passées du climat. Ces études ont permis de déterminer la direction et la vitesse de migration des espèces, données utilisées actuellement pour générer des prédictions sur l'évolution de l'aire de répartition des espèces forestières en réponse au réchauffement du climat. Cependant, le contexte écologique dans lequel les arbres font face à ces changements est très différent par rapport aux changements climatiques passés : l'augmentation actuelle des températures est plus rapide, les surfaces susceptibles d'être colonisées sont occupées par des écosystèmes très différents et variés (forêts, surfaces agricoles, zones urbaines). Par conséquent, les arbres pourront-ils faire face à la rapidité des changements globaux actuels ? Auront-ils la capacité de migrer pour trouver des conditions plus favorables ou pourront-ils s'adapter et survivre à de nouvelles conditions environnementales ? Dans un premier temps, l'analyse de données historiques (plans d'aménagements de l'Office Nationale des Forêts et Inventaires Forestiers Espagnols) a permis de mettre en évidence des évènements de colonisation et d'extirpation et de quantifier la vitesse de migration de populations situées au cœur ou aux marges de leur aire de répartition. Une colonisation massive de Quercus ilex dans les dunes boisées atlantiques (limite Nord d'aire de répartition) a été mise en évidence au cours des 130 dernières années, confirmant les tendances prédites par les modèles. Cependant, les vitesses de colonisation de cette espèce restent bien inférieures aux déplacements de son bioclimat estimés à partir de modèles de niche. Les espèces localisées en limite Sud d'aire de répartition présentent des remontées altitudinales plus importantes que pour celles situées au cœur de leur aire. En conclusion, nos résultats montrent que les changements globaux ont déjà impactés la répartition des arbres malgré l'existence d'un décalage temporel entre les réponses migratoires des espèces forestières et le déplacement de leur bioclimat. Le stress hydrique est le facteur prépondérant pouvant expliquer le dépérissement des arbres dans un milieu dont la disponibilité en eau est limitée, notamment en marge chaude d'aire de répartition. Nous avons, par conséquent, étudié la résistance à la sécheresse et ses mécanismes chez les plusieurs espèces d'Angiospermes. Nos résultats montrent qu'un seuil de 90% d'embolie mène à des dommages physiologiques irréversibles de la plante et à la mort par déshydratation. Ce seuil est considérablement plus élevé que celui précédemment observé chez les conifères. L'étude du fonctionnement hydraulique d'espèces de chênes co-occurrentes nous a permis de montrer que la survie de Q. robur pourrait être menacée dans les forêts atlantiques dans un contexte de sécheresses de plus en plus intenses car il y subit des taux d'embolie native élevés. Au contraire, Q. ilex présente des taux d'embolie négligeables sur ce même site d'étude.Les vitesses réelles de migrations constituent des données empiriques essentielles qui nous renseignent sur les capacités migratoires effectives des arbres. Elles pourront être intégrées dans les modèles de répartition, tout comme les seuils d'embolie induisant la mort des arbres.

L’écologie du Desman des Pyrénées (Galemys pyrenaicus), mammifère semi-aquatique endémique de la péninsule ibérique et des Pyrénées, demeure encore très peu connue. Les objectifs de cette thèse, dans le cadre d’un Plan National d’Actions, ont été d’identifier les variables environnementales agissant sur la répartition de l’espèce à différentes échelles spatiales, en considérant sa détectabilité imparfaite (i.e. fausses absences et fausses présences). Une probabilité de détection élevée, mais spatialement hétérogène à l’échelle des Pyrénées françaises, a été mise en évidence. La distribution du Desman des Pyrénées s’est également révélée spatialement structurée et majoritairement influencée par des facteurs propres aux milieux aquatiques, mais en forte régression depuis les années 80. Ces résultats ont permis de proposer des mesures de conservation pour cette espèce menacée.

Plants respond to changes in their local environment and, at the same time, influence the environment at a global scale. The molecular and physiological mechanisms regulating this interaction are not completely understood and this limits our capacity to predict the response of vegetation to future environmental changes. This dissertation combined tools from genomics, physiology, and ecology to examine the response of plants to environmental change. Specifically, it focused on processes affecting carbon and water exchange in forest trees because (1) trees are long-lived species that might face repeated environmental challenges; (2) relatively little information exists about the genes and the molecular mechanisms regulating structural and physiological traits in adult, long-lived woody plants; and (3) forest trees exchange a significant amount of carbon and water with the atmosphere and are therefore major players in the global carbon and water cycles.

Water flux through forests depends both on environmental conditions (e.g., soil moisture) and on the hydraulic architecture of individual trees. Resistance to xylem cavitation is an important hydraulic trait that is often associated with drought tolerance but potentially at the cost of reduced carbon uptake. The second chapter of this dissertation evaluated the variation in resistance to xylem cavitation, hydraulic conductivity, wood anatomy traits, and leaf gas exchange across 14 co-occurring temperate tree species including both angiosperms and gymnosperms. The relationship between vulnerability to cavitation (ψ₅₀) and hydraulic conductivity within specific organs (i.e. stems and roots) was not significant when considering the phylogenetic association between species. However, even after phylogenetic correction, photosynthetic carbon uptake (A) was positively correlated with both stem and root ψ₅₀, and stomatal conductance (g_s ) was strongly correlated with root ψ₅₀ . These results suggest that there is a trade-off between vulnerability to cavitation and water transport capacity at the whole-plant level, and that this functional relationship reflects an adaptive response to the environment.

Forests are an important component of the global carbon cycle that can be directly impacted by a rise in atmospheric CO₂ concentration.. The third chapter of this dissertation investigated the effects of long-term exposure to elevated CO2 on the gene expression of mature, field-grown loblolly pine trees. Using cDNA microarrays, I compared the expression of 1784 pine transcripts in trees growing under ambient and those under elevated CO₂ at monthly intervals throughout a growing season. Overall, more genes were upregulated than downregulated by elevated CO₂, although the total number of genes differentially expressed varied throughout the season. The pattern of increasing number of differentially expressed genes until the peak of the growing season (July and August) followed by a decrease in that number, matched the seasonal trend of tree growth and photosynthetic response to elevated CO₂ in this species. The seasonal trend also reflected the interaction among multiple abiotic factors intrinsic to field conditions and emphasized the relevance of evaluating the role of genes in their natural environment. Genes consistently upregulated by elevated CO₂ were functionally associated with environmental sensing, cellular signaling, and carbon metabolism, in particular the degradation of carbohydrates through respiration. An increase in carbohydrates degradation is particularly relevant in the context of carbon balance of forest trees because of the potential for enhanced leaf and tree respiration leading to a reduced sink capacity for CO₂.

Loblolly pine produces several flushes of needles throughout the year each with an average lifespan of 19 months. Each year, two age classes of needles contribute to the annual carbon sequestration of the loblolly pine forest. To address the impact of leaf age on the effects of elevated CO₂ in carbon metabolism regulation, I compared the gene expression profiles from trees under ambient and elevated CO₂ conditions in two needle cohorts: one-year-old and current-year. Differential expression under elevated CO₂ was seven times more frequent in current-year than in one-year-old needles. Despite differences in magnitude, many of the patterns within specific groups of genes were similar across age classes. For instance, there was a trend for downregulation of genes involved in the light-reactions of photosynthesis and those in photorespiration in both age classes, while genes associated with dark respiration were largely upregulated by elevated CO₂ in both cases. The difference between the two cohorts was particularly evident in the group of genes related to energy production (ATP synthesis) and the group associated with carbon partitioning (sucrose and starch metabolism). Because sucrose and starch metabolism categories included many genes known to be important regulators of gene expression and plant physiological processes, this suggests that this stage of carbon metabolism might be an important control point in age-dependent foliar responses to elevated CO₂.

This dissertation examined both structural and physiological components of plant water and carbon relations (Chapter 2) across different biological scales of organization (whole-plant level in Chapter 2; gene-level response to ecosystem-level changes in Chapters 3 and 4) and reflecting adjustments at distinct temporal scales (life-span of the organism vs. evolutionary selection of traits). An integrative approach was used to advance our understanding of how plants acclimate and adapt to their environment, and to provide a mechanistic framework for predictive models of plant response to environmental change.

Dissertation

Since the pre-industrial age, the Earth has been warming at unparalleled rates, and this warming is changing climate and weather, creating a more extreme global hydrological cycle. In this dissertation, I explore how these changes to the hydrological cycle may act ecosystem and community level responses of terrestrial plants in the Midwestern United States. In this region, it is projected that mean annual precipitation (MAP) will increase, but precipitation will become more variable across and within seasons. Ecosystem structure and function are vulnerable to changes in hydrologic patterns, including changes in biogeochemical cycles, plant productivity, and plant community structure and function. In this dissertation, I explore how changes in precipitation will alter these processes using two field experiments, and I suggest potential hypotheses that could explain drought-induced community change.

In chapter 1, I explore how alterations to seasonal precipitation in the winter and summer act ecosystem and community processes in a temperate deciduous forest. Biogeochemical processes and plant communities are sensitive to changes in abiotic conditions, and these conditions will alter forest succession, particularly juvenile woody plant species. Using a fully factorial experiment, I manipulated winter snowfall and summer precipitation to create wet, dry, and control (ambient conditions) treatments and investigated how changes in seasonal precipitation would act mineralization rates, woody plant recruitment, and understory composition. I found that the effects of winter and summer precipitation on these processes acted independently of one another in this system, and the system was resistant to changes in mineralization rates and understory composition. Woody plant recruitment may be more sensitive to altered precipitation, as recruitment of at least one of the four species planted, Lindera benzoin, was impacted by changes in seasonal precipitation. Snow removal treatments reduced germination and increased summer precipitation decreased the relative growth rate of this species. In the short term, slight changes to woody plant recruitment may have little impact on long-term forest succession, but as these changes persist over longer periods of time, they could alter the direction of succession, which could lead to changes in the understory community composition and nutrient cycling.

The second and third chapters explore the effects that drought intensification will have on terrestrial plant communities. Numerous studies have investigated the effects of individual droughts on ecosystem and community responses, but the effects that both the timing and duration of drought have on these responses remain largely unknown. To explore this gap in the literature, I conducted a eld experiment using rainout shelters to reduce growing season precipitation, creating dry periods that varied in length and timing. Drought can impact productivity and diversity in this system, and the timing in which the drought occurs influences these effects. Surprisingly, I found that the length of drought did not affect productivity or community composition.

The final chapter introduces the Community Response to Extreme Drought framework CRED), which addresses the potential temporal progression of mechanisms and plant-plant interactions that may lead to community changes during and after a drought. The mechanisms for the temporal evolution of community-level drought responses are not fully understood, but plant-plant interactions, both competitive (-) and facilitative (+), are increasingly being recognized as important drivers of community compositional changes. The CRED framework provides hypotheses for the roles that plant-plant interactions have on drought-induced community change. CRED addresses how system-specific variables and the intensity of drought may influence the strength of plant-plant interactions over time, and ultimately the systems resistance and resilience to drought.

The results from this dissertation work have revealed that more research needs to be done to fully understand how changes in precipitation regimes and patterns will affect terrestrial ecosystems and plant communities. A better understanding of how ecosystems and communities respond to drought timing and length can help improve climate models and restoration strategies.

This dissertation examines issues concerning sap flux scaled estimates of the canopy-averaged transpiration rate of trees per unit leaf area (E_L) and stomatal conductance (G_S), as well as their implications in the water and carbon balance of individuals and stands, with the final goal of an integrated assessment of 11 years of such data from two species (Pinus taeda and Liquidambar styraciflua) at the Duke Free Air Carbon dioxide Enrichment (Duke FACE) facility. These issues include (1) the effects of allometric relationships and xylem characteristics on the gas phase transport of water from leaves and the hydraulic supply of it, (2) consideration of the hydraulic capacitance in the inference of stomatal behavior from sap flux data and (3) the dynamic modeling of stomatal conductance to environmental drivers using Bayesian techniques. It is shown that a) for resolution of sap flux in conifers at the scale of minutes under dynamic conditions, time constants for both stomatal responses and hydraulic capacitance of sapwood must be considered, (b) nighttime conductance can lead to large errors in rates of sap flux measured under some conditions, (c) variation in allometry between P. taeda individuals can lead to different rates of transpiration and carbon assimilation per unit leaf area and that (d) hydraulic time constants for the stems of mature P. taeda at Duke FACE trees varied by the stem length considered and were on the order of 30-45 minutes for a 10-m segment. An analysis incorporating all these elements leads to the conclusions that (e) both elevated CO₂ (eCO₂) and fertilization (FR) resulted in proportionally larger reductions in the E_L and G_S of P. taeda as soil moisture decreased with (f) eCO₂ having little to no effect in months of high soil moisture and (g) FR leading to ~14% reduction of GS under high soil moisture in absence of eCO₂, while (h) both eCO₂ and FR led to reduced E_L and G_S of L. styraciflua across soil moisture conditions.

Dissertation

Global warming is forecast to cause significant thawing of the permafrost that surrounds lakes and rivers across the Arctic, with potential wide-scale effects on the water quality and biotic characteristics of these water bodies. The benthic environment is believed to be especially sensitive to permafrost-induced ecological change, and this has been the focus of recent field intensive research. Five lakes disturbed and three lakes undisturbed by retrogressive permafrost thaw slumps were sampled during late summer of 2006 to assess the potential effects of slumping on benthos. Water quality parameters, submerged macrophytes, benthic invertebrates, and sediment were collected. A significant difference (p < 0.05) between disturbed and undisturbed lakes was found for macrophyte, invertebrates, underwater light attenuation, and some sediment variables. The results suggest that thaw slumps can affect submerged macrophyte biomass, benthic invertebrate abundance, and also community structure in upland tundra lakes. Such differences between undisturbed and disturbed lakes are suggested to be related to changes in water column transparency, sediment nutrient availability, soil and terrestrial vegetation loading from the landscape, and changes in slope angle of the littoral zone.

Management of predation on sea turtle nesting beaches is vital to conservation efforts for the vulnerable loggerhead turtles (Caretta caretta) and olive ridley turtles (Lepidochelys olivacea). Sea turtles increasingly face threats from invasive and human-tolerant mammalian predators as human disturbances on nesting beaches rises. The intensity of mammalian predation has increased in Las Baulas National Park in Costa Rica which is an important nesting site for several species of threatened and endangered sea turtles. I analyzed loggerhead and olive ridley nest predation on four beaches in the United States and Costa Rica that were chosen for variations in degree of human disturbance and management strategies. My objectives were to 1) determine if egg predation rates differ at the four sites, 2) determine the most destructive predators at each location, and 3) suggest management options to alleviate mammalian threats to turtle clutches on Playa Grande and Playa Cabuyal in Costa Rica. My results show that the beaches without a nest protection or predator control program had very high rates of predation. Invasive mammalian predators and mammalian predators associated with human disturbance were the most destructive at the four sites. I recommend that regulations regarding dogs and the take of eggs from the beach are enforced at Playa Cabuyal and that physical nest protection is rapidly implemented at Playa Grande. I also recommend that the National Park consider managing raccoon predation by removing problem individuals, but caution that they do so in a way that maintains the animals’ role in the ecosystem.

Eastern black walnut (Juglans nigra L.) ranks among the most highly valued timber species in the central hardwood forest and across the world. This valuable tree fills a critical role in native ecosystems as a mast bearing pioneer on mesic sites. Along with other Juglans spp. (Juglandaceae), J. nigra is threatened by thousand cankers disease (TCD), an insect-vectored disease first described in 2009. TCD is caused by the bark beetle Pityophthorus juglandis Blackman (Corthylini) and the phytopathogenic fungus Geosmithia morbida Kol. Free. Ut. & Tiss. (Bionectriaceae). Together, the P. juglandis-G. morbida complex has expanded from its historical range in southwest North America throughout the western United States (U.S.) and Europe. This range expansion has led to widespread mortality among naïve hosts J. nigra and J. regia planted outside their native distributions.

The severity of TCD was previously observed to be highest in urban and plantation environments and outside of the host native range. Therefore, the objective of this work was to provide information on biotic and abiotic environmental factors that influence the severity and impact of TCD across the native and non-native range of J. nigra and across different climatic and management regimes. This knowledge would enable a better assessment of the risk posed by TCD and a basis for developing management activities that impart resilience to natural systems. Through a series of greenhouse-, laboratory- and field-based experiments, environmental factors that affect the pathogenicity and/or survival of G. morbida in J. nigra were identified, with a focus on the microbiome, climate, and opportunistic pathogens. A number of potentially important interactions among host, vector, pathogen and the rest of the holobiont of TCD were characterized. The holobiont is defined as the whole multitrophic community of organisms—including J. nigra, microinvertebrates, fungi and bacteria—that interact with one another and with the host.

Our findings indicate that interactions among host, vector, pathogen, secondary pathogens, novel microbial communities, and novel abiotic environments modulate the severity of TCD in native, non-native, and managed and unmanaged contexts. Prevailing climatic conditions favor reproduction and spread of G. morbida in the western United States due to the effect of wood moisture content on fungal competition. The microbiome of soils, roots, and stems of trees and seedlings grown outside the host native range harbor distinct, lower-diversity communities of bacteria and fungi compared to the native range, including different communities of beneficial or pathogenic functional groups of fungi. The pathogen G. morbida was also associated with a distinct community of microbes in stems compared to G. morbida-negative trees. The soil microbiome from intensively-managed plantations facilitated positive feedback between G. morbida and a disease-promomting endophytic Fusarium solani species complex sp. in roots of J. nigra seedlings. Finally, the nematode species Bursaphelenchus juglandis associated with P. juglandis synergizes with G. morbida to cause foliar symptoms in seedlings in a shadehouse; conversely, experiments and observations indicated that the nematode species Panagrolaimus sp. and cf. Ektaphelenchus sp. could suppress WTB populations and/or TCD outbreaks.

In conclusion, the composition, function, and interactions within the P. juglandis and J. nigra holobiont play important roles in the TCD pathosystem. Managers and conservationists should be aware that novel associations outside the host native range, or in monocultures, intensive nursery production, and urban and low-humidity environments may favor progression of the disease through the effects of associated phytobiomes, nematodes, and climatic conditions on disease etiology. Trees in higher diversity, less intensively managed growing environments within their native range may be more resilient to disease. Moreover, expatriated, susceptible host species (i.e., J. nigra) growing in environments that are favorable to novel pests or pest complexes (i.e., the western U.S.) may provide connectivity between emergent forest health threats (i.e., TCD) and native host populations (i.e., J. nigra in its native range).

Human society is presently beset by an array of anthropogenic social-ecological crises that threaten the sustainability of the social-ecological systems that sustain our livelihoods. While research alone will not rectify these issues, it can help to answer key questions that must be addressed to develop effective solutions. To address such questions in a cohesive, compelling manner, social-ecological research can be bounded, structured, and distilled through innumerable organizing principles or theoretical frameworks. For this dissertation, I focused on the geographic region of Tierra del Fuego and sought to draw from the array of disciplines and methods that use sound as a lens for biological, ecological, and/or social inquiry. I also endeavored to consider various temporal, spatial, and organizational scales while investigating a selection of topics with a) specific importance in the social-ecological systems of Tierra del Fuego and b) general relevance to global social-ecological challenges. Chapter 1 provides an introduction to the dissertation, and Chapter 6 serves as a conclusion.

The objective of Chapter 2, “Biogeographical and analytical implications of temporal variability in geographically diverse soundscapes”, was to provide some guidance to passive acoustic monitoring (PAM) practitioners on how to design appropriate temporal sampling schemes based on the temporal variability of the sounds one wishes to measure and the power and storage limitations of acoustic recorders. We first quantified the temporal variability of several soundscape measurements and compared that variability across sites and times of day. We also simulated a wide range of temporal sampling schemes in order to model their representativeness relative to continuous sampling.

For Chapter 3, “Sentinels for sentinels: passive acoustic and camera trap monitoring of sensitive penguin populations”, we tested the utility of PAM to monitor behavior and abundance of Magellanic (Spheniscus magellanicus) and southern rockhopper penguins (Eudyptes chrysocome) at different spatial and temporal scales. We conducted in situ observations of the acoustic behavior of each species, and we compared acoustic metrics with penguin counts from narrowly focused camera traps and larger-extent observations of colony density.

Chapter 4, “Acoustic monitoring shows invasive beavers (Castor canadensis) increase avian diversity in Tierra del Fuego”, is focused on impacts of the invasive North American beaver (Castor canadensis) on Fuegian bird communities. We sought to determine how bird communities might differ between intact riparian forests, beaver ponds, and beaver meadows created by pond drainage. We conducted PAM and classic avian point counts under each of these conditions across seasons to test for differences between impact conditions and to compare the two methodologies.

For Chapter 5, “Human-nature connection and soundscape perception: insights from Tierra del Fuego, Argentina”, we evaluated the relationship between soundscape perception and nature relatedness by conducting surveys and soliciting responses to soundscape audio prompts. We also examined the potential for any demographic influences on nature relatedness or soundscape perception in the context of local social tensions.