Dissertations / Theses on the topic '060802 Animal Cell and Molecular Biology'
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Siam, Rania. "Mechanisms of C. crescentus regulation of chromosome replication by a cell cycle regulator protein." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=37838.
Full textThis cooperative CtrA binding at [a] and [b] is independent from the upstream binding sites [c-e] (Chapter 2). CtrA∼P binding in the origin is altered in the presence of the histone-like protein (IHF) that also binds and overlaps CtrA binding site [c] (Chapter 5). In-fact, IHF binds and overlaps binding site [c] (Chapter 5). We propose a replication model in the stalked cell were IHF binding hinders active CtrA binding in the replication origin and regulates cooperative transcription that coincides with replication initiation.
Nguyen, Hannah Anh-Quan. "Regulation of gene expression and cell growth by transcriptional proteins of the interferon system." Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=35028.
Full textSaleh, Maya. "Protein-protein interactions and cell signaling in the regulation of HOX.PBX functions." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=37620.
Full textBrule, Veronique. "The role of RBF1 in animal survival and in male germline stem cell differentiation during Drosophila melanogaster development." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119514.
Full textLe rétinoblastome (RB) est une protéine classifiée comme suppresseur de tumeur. Elle joue plusieurs rôles importants dans la cellule, incluant: la régulation du cycle cellulaire, la différentiation cellulaire et l'apoptose. Dès sa découverte, le rétinoblastome a été caractérisé par son rôle primaire en règlant le cycle cellulaire dans lequel RB fonctionne comme régulateur de la transition entre la phase G1 et la phase S. Depuis ce temps, d'autres rôles pour RB indépendents de son fonctionnement dans le cycle cellulaire ont été identifiés. Notamment, il a été démontré que RB joue un rôle important dans la promotion de processus de dévelopement normal dans l'animal et la survie jusqu'à l'âge d'adulte. La plupart des recherches concentrant sur ce rôle ont été faites en utilisant la Drosophile, une espèce modèle qui convient à la manipulation génétique et en laquelle l'homologue de RB se nomme RBF1.Il reste plusieurs questions à rechercher à propos du rôle de RBF1 concernant la survie de l'animal jusqu'à l'âge d'adulte. Cette thèse essaie de répondre à la question suivante; est-ce que les effets biologiques résultant de mutater RBF1 sont spécifiques à des tissus particuliers, et est-ce qu'ils ont un effet sur l'abilité de la Drosophila de survivre jusqu'au stage d'adulte? Les résultats de cette thèse indiquent qu'en mutant RBF1, il est possible de produire des effets biologiques unique dans des tissus spécifiques. Quand même, aucun de ces effets ont affecté la survie de l'animal. Par example, il a été démontré que RBF1 joue un rôle unique dans le tissu gonade des mâles. Dans des Drosophiles qui n'expriment pas RBF1 dans les cellules de la lignée germinale, les adultes mâles étaient stériles. En examinant les testicules, le phénotype des cellules de la lignée germinale était différent en comparaison au phenotype sauvage. Aussi, le nombre de cellules da la lignée germinale qui expriment la cycline E avait augmenté. Précédemment, il n'y avait pas d'études qui ont recherché un rôle pour RBF1 dans la lignée germinale des Drosphiles mâles; donc, les résultats de cette thèse ont démontré un rôle unique pour RBF1 en régularisant la spermatogenèse dans les Drosophiles.En plus, cette thèse essaie de répondre à une autre question liée à celle ci-haut: est-ce que RB s'associe avec des macromolécules particulières (dont il est déjà connue à faire des associations), mais d'une manière unique à chaque tissu de la Drosophile? Les données de cette thèse ne donnent pas une réponse complète à cette question, mais elles ont identifié in vivo un site de phosphorylation (Sérine 728) sur RBF1. Ce site a déjà été recherché et a été suggéré in vitro d'être une cible de phosphorylation par les complexes Cdk-cyclines. Alors, les données présentées ci-haut démontrent que Sérine 728 peut être phosphorylé in vivo aussi. Plus de recherche est requis pour vérifier si ce site est un cible authentique de phosphorylation endogène in vivo. En conclusion, les données de cette thèse démontrent de nouvelles façons de régler le fonctionnement de RBF1, et elles présentent des informations nouvelles à propos des rôles variés de RBF1 dans la Drosophile.
Knudson, Jennifer Caroline. "Cardiotrophin-1 as an ex vivo activator of a stem-cell like population in the murine heart." Thesis, University of Ottawa (Canada), 2005. http://hdl.handle.net/10393/26946.
Full textWang, Yifang. "Role and regulation of X-linked inhibitor of apoptosis protein expression during development of the rat ovarian follicle in vitro." Thesis, University of Ottawa (Canada), 2003. http://hdl.handle.net/10393/29006.
Full textZhao, Qing 1966. "Prosaposin : a glycoprotein with multiple functions and dual destinations." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=36857.
Full textAn, Jing 1962. "Microenvironmental influences on the growth of normal and leukemic myeloid cells in the rat bone marrow." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41964.
Full textMillion, Passe Christina M. "Role and regulation of the high mobility group protein p8 in gonadotrope development, function, and tumorigenesis." [Bloomington, Ind.] : Indiana University, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3330816.
Full textTitle from PDF t.p. (viewed on Jul 23, 2009). Source: Dissertation Abstracts International, Volume: 69-10, Section: B, page: 5924. Adviser: Christine Quirk.
Springer, Lisa Nicole 1966. "Cellular and molecular mechanisms of 4-vinylcyclohexene-diepoxide induced ovotoxicity in rats." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/282161.
Full textChu, Angel On Kei 1975. "The effect of stress on nuclear transport and nuclear organization /." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82846.
Full textI have demonstrated that classical nuclear import is inhibited by oxidative stress in living HeLa cells as a result of relocalization and degradation of nuclear factors important for the nuclear transport apparatus. Specifically, hydrogen peroxide redistributes Ran, the GTPase important for the directionality of transport, importin-beta, a subunit of the nuclear import receptor, and Nup153, a component of the nuclear pore complex. Moreover, the stress-induced relocalization and degradation does not rely on the activation of MAPK pathways.
Heat shock proteins have established roles in normal cellular homeostasis as well as in stress response. In unstressed conditions, proteins of the hsp70/hsc70 family shuttle between the nucleus and the cytoplasm. Upon stress, cytoplasmic hsp70s/hsc70s accumulate in the nucleus. I have further characterized the effect of stress on hsc70s localization in HeLa cells. Heat-induced nuclear concentration of hsc70s depends on cell density. Moreover, protein phosphorylation negatively regulates hsc70 nuclear accumulation in response to heat. During recovery from heat stress hsc70s redistribute as they are exported into the cytoplasm. Hsc70 export is temperature- and energy-dependent, but is independent of the Crm1/exportin1-mediated pathway. Moreover, export of hsc70 is inhibited by depolymerization of nuclear actin.
In higher eukaryotes, lamins and other lamina-associated proteins provide links between the nuclear envelope and chromatin. The protein circumferin is located at the nuclear periphery in yeast and higher eukaryotes. I have demonstrated that stress relocalizes circumferin, which is released from the nuclear periphery into the nucleoplasm upon heat shock. These studies are the first to demonstrate that nuclear envelope organization in yeast and mammalian cells is modulated by stress.
Taken together, these results indicate that both nuclear transport and nuclear organization are affected by stress.
Sutherland, Vicki Lynn. "Characterization of glucose sensing neuroendocrine cells." Diss., The University of Arizona, 2002. http://hdl.handle.net/10150/280211.
Full textShi, Niu 1963. "Characterization of MEQC and functional studies of glypican and p23." Diss., The University of Arizona, 1997. http://hdl.handle.net/10150/282305.
Full textLiu, Bin 1960. "Studies of parathyroid hormone related peptide : gene expression,biosynthesis, and processing." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=39948.
Full textsupsavhad, wachiraphan. "Novel Molecular Targets for Feline Oral Squamous Cell Carcinoma." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1471628009.
Full textSong, Xiaozheng. "Estrogen Receptor Beta Is A Negative Regulator Of Mammary Cell Proliferation." ScholarWorks @ UVM, 2014. http://scholarworks.uvm.edu/graddis/259.
Full textMartens, Adam Arai. "Caracterização do gene CDK7 e análise de sua possível atuação nos endociclos de Rhynchosciara americana." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-26072012-142501/.
Full textCDKs are proteins responsible for activation and progression of cell cycle and also work on the activation and elongation of transcription. Among them, CDK7 acts in both functions, phosphorylating cell cycle CDKs and as a part of general transcription factor TFIIH as its catalytic subunit. From an EST library of salivary gland, constructed with mRNAs present during the period when the beginning of the last polyteny replicative cycle occurs, it was found only few messages directly related to cell cycle, corresponding to 3.11% of the ESTs. Among them, it was found Cdc2-like and Cdk7; therefore, it was performed the characterisation of Cdk7 gene and the analysis of its role during the larval development of Rhynchosciara americana. Cdk7 gene presents 4 exons, more than in vertebrates. Complete mRNA sequence was obtained via RACE, presenting 1230 bases and an 1020 bases ORF. Expression profiles were determined by RT-PCR and Western blots. Posttranslational modifications were analysed by 2D immunoblots. Its mRNA and protein expression profiles presented variations during cell cycle and between the studied tissues; immunoblots showed the presence of one phosphorylation and possible modifications on the side chain of some amino acids. The study of proteins related to cell cycle in this model is important for a better understanding of uncommon cell cycles in different insect tissues.
Lu, Zhongyan. "Genetic Mechanisms of Porcine Sapovirus Adaptation to Cell Culture." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1449149533.
Full textROSAURO, CRISTIANE W. "Altos niveis de expressao de hormonio de crescimento de camundongo em queratinocitos humanos visando a obtencao de um modelo animal de terapia genica." reponame:Repositório Institucional do IPEN, 2003. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11151.
Full textMade available in DSpace on 2014-10-09T14:01:11Z (GMT). No. of bitstreams: 1 09618.pdf: 4753745 bytes, checksum: f0c21d288bbe1bd9370a02c1b417d561 (MD5)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Dissertacao (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
FAPESP:00/08457-0
Head, Talia B. "Proteomic Analysis of the Crustacean Molting Gland (Y-Organ) Over the Course of the Molt Cycle." DigitalCommons@CalPoly, 2017. https://digitalcommons.calpoly.edu/theses/1830.
Full textVallaster, Markus Parzival. "Intergenerational Effects of Nicotine in an Animal Model of Paternal Nicotine Exposure." eScholarship@UMMS, 2017. https://escholarship.umassmed.edu/gsbs_diss/913.
Full textVallaster, Markus Parzival. "Intergenerational Effects of Nicotine in an Animal Model of Paternal Nicotine Exposure." eScholarship@UMMS, 2008. http://escholarship.umassmed.edu/gsbs_diss/913.
Full textNam, Minwoo. "Role of Energy Metabolism in the Thermogenic Gene Program." eScholarship@UMMS, 2017. http://escholarship.umassmed.edu/gsbs_diss/886.
Full textYang, Liqun. "Characterization of the Physiologic Function of NF-κB2 p100." University of Toledo Health Science Campus / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=mco1263334529.
Full textZhang, Jibin. "Identification of Important Cell Cycle Regulators and Novel Genes in Specific Tissues using Microarray Analysis, Bioinformatics and Molecular Tools." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429637289.
Full textLinhares, Boakari Yatta. "EFFECTS OF A SYSTEMIC HIGH UREA CONCENTRATION ON THE ENDOMETRIAL AND EMBRYONIC TRANSCRIPTOMES OF THE MARE." UKnowledge, 2019. https://uknowledge.uky.edu/gluck_etds/42.
Full textHuisken-Hill, Alyse Lynn. "Influencing Pathways that Cause Metastasis and Stemness in Epithelial Ovarian Cancer." CSUSB ScholarWorks, 2016. https://scholarworks.lib.csusb.edu/etd/355.
Full textKeller, Emma Jean. "The Contribution of IFNα-Stimulated Immune Cell Populations to B6.NbA2 Lupus-likeDisease." Case Western Reserve University School of Graduate Studies / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1625138193480211.
Full textIscaife, Alexandre. "O uso de microRNA para tratamento do câncer de próstata: estudos in vitro e in vivo." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5153/tde-22082016-104926/.
Full textIntroduction: Prostate cancer (PCa) is the most common neoplasia of man in Western countries and the second cause of death by cancer in men in the US, Europe and Brazil. The localized cancer has high cancer-specific survival when treated properly, however metastatic disease still presents low effective treatments with 28% of global survival. microRNAs (miRNAs) are a group of small RNA molecules containing from 19 to 25 nucleotides of noncoding protein with fundamental action in the regulation of gene expression. They are involved in key processes in normal and neoplastic cells as cell cycle, proliferation, apoptosis, energy metabolism, invasion and metastasis. Objectives: To carry out studies in vitro and in vivo using miRNA in a novel model of metastatic prostate cancer in our country in order to evaluate its potential as a therapeutic agent of this neoplasia. Methods: In the in vitro studies, three cell lines were used (PC3, DU145 and LNCaP). These cell lines were transfected with miRNAs 100, 145 and 373 and their antiMiRs using lipofectamine. We analyzed the gene expression of mTOR, SMARCA5, KRAS, CMYC, MMP9, CD44 by real-time polymerase chain reaction (qRT-PCR). We also performed studies of apoptosis, cell cycle and ploidy using flow cytometer. Changes in the invasion potential were evaluated by the technique of matrigel. The pre-clinical model in vivo was developed by intracardiac injection of PC-3MLuc-C6 cell line in NUDE mice with 9 weeks. Tumor growth was evaluated with an in vivo image system (IVIS). After the full establishment of metastases on day 21, the animals were treated with three injections into the tail vein containing the miRNA plus atelocollagen. The animals were sacrificed on day 48 for tissues analysis. Results: MiR-100 increases apoptosis in LNCaP and reduces apoptosis in DU145. The anti-miR-100 increased apoptosis in 14% in PC3. In cell line DU145, miR-100 inhibited proliferation. In the analysis of gene expression, the miR-100 inhibits SMARCA5 in DU145 and PC3 and mTOR in LNCaP, anti-miR-100 stimulates mTOR and SMARCA5 in LNCaP. The miR-145 promoted an increased in apoptosis by 24% in DU145. In PC3 cell line miR-145 acts by inhibiting the proliferation, with an absolute difference of 18% compared to control. MiR-145 inhibits KRAS and CMYC in the three cell lines and anti-miR-145 stimulates CMYC in DU145 and KRAS in the three cell lines. The miR-373 reduced apoptosis by 29% in DU145 and reduces proliferation with an absolute difference of 13% relative to control. MiR-373 stimulates MMP9 in DU145 and LNCaP cells and inhibits CD44 in PC3. The anti -miR-373 inhibits MMP9 in DU145 and LNCaP. In the in vivo studies of metastatic PCa, miR-100 shows a tendency to decrease tumor growth (p=0.23) and miR-145 reduces tumor growth on day 34 (p=0.02). After those days, the tumor grows back aggressively. Animals treated with anti-miR-373 showed no changes relative to controls. Conclusion: The miR-100 is a context-dependent miRNA, with tumor suppressor role in aggressive tumor cell lines. The miR-373 acts in vitro as oncomiR and miR-145 acts as a tumor suppressor in vitro and in an animal model with consistent therapeutic response and can be used in the therapeutic arsenal against this neoplasia. Future studies should evaluate the use of miRNAs alone or adjuvant in the treatment of metastatic prostate cancer
Cellurale, Cristina Arrigo. "Role of the cJun NH2-Terminal Kinase (JNK) in Cancer: A Dissertation." eScholarship@UMMS, 2010. https://escholarship.umassmed.edu/gsbs_diss/478.
Full textJacobs, Maria-Flora. "Effects of Aquatic Acidification on Calcium Uptake in White River Shrimp Litopenaeus setiferus Gills." UNF Digital Commons, 2019. https://digitalcommons.unf.edu/etd/870.
Full textPERRIN, CHRISTELE. "Methodologie pour l'analyse quantitative en imagerie microscopique conventionnelle et a fluorescence. Application a l'etude de la proliferation et de l'expression du recepteur a l'egf dans des cellules tumorales mammaires." Université Joseph Fourier (Grenoble), 1996. http://www.theses.fr/1996GRE10198.
Full textHenninger, Nils. "Inhibiting Axon Degeneration in a Mouse Model of Acute Brain Injury Through Deletion of Sarm1." eScholarship@UMMS, 2017. http://escholarship.umassmed.edu/gsbs_diss/900.
Full textMyler, Heather Ann. "Heparanase and platelet factor-4 induce smooth muscle cell proliferation and migration via basic fibroblast growth factor release from the extracellular matrix: Implications in the restenosis process." Thesis, 2003. http://hdl.handle.net/1911/18597.
Full text"Integrated hormonal control of growth hormonemRNA expression in the MtT/S somatotroph cell line." Tulane University, 2000.
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"Molecular and electrophysiological studies of neuronal A-type and M-type K(+) channels." Tulane University, 2002.
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(11177052), Shashank Manohar Nambiar. "Maternal Hepatic Adaptations to Pregnancy." Thesis, 2021.
Find full textDuring gestation, the maternal liver undergoes various adaptive changes to cope with the increasing physiological and metabolic demands from both maternal and fetal compartments. Among these changes are robust growth and changes in transcriptome profile. However, how these events happen, and other aspects of this physiological phenomenon remains unexplored. Therefore, we aimed at further understanding how maternal liver responds to pregnancy. We used BrdU labeling combined with a virus-based tracing approach to quantify the percentage of maternal hepatocytes undergoing DNA synthesis and division over the course of gestation in mice.
We found that ~50% maternal hepatocytes entered S-phase but, unexpectedly, did not undergo cytokinesis. This strongly suggests that maternal hepatocytes in fact undergo endoreplication instead of hyperplasia, as believed previously. Pericentral Axin2+ hepatocytes were reported to behave as liver stem cells responsible for liver homeostasis and turnover. We generated an in vivo fate-tracing mouse model to monitor the behavior of these cells in the maternal liver. Our results showed that they did not proliferate during pregnancy, homeostasis, and following partial hepatectomy. Curiously, we uncovered that, hepatocytes exhibit developmental phenotypes at mRNA level pre-pregnancy and at both mRNA and protein level during pregnancy. In the non-pregnant state, hepatocytes reserved mRNA expression of liver progenitor marker genes Cd133 and Afp, which are localized in the nuclei, without protein translation. During gestation, maternal hepatocytes displayed cytoplasmic translocation of Cd133 and Afp transcripts, concomitant with corresponding protein expression.
Overall, all maternal hepatocytes became CD133+, and a subset of them express AFP. Additionally, in non-pregnant livers, mRNA of Epcam, another liver progenitor marker, was expressed within majority of hepatocytes, whereas its protein was solely translated in the pericentral region. In contrast, by end-gestation, EPCAM protein expression switched to the periportal region. These observations indicate that maternal hepatocytes exhibit heterogeneous developmental phenotypes, partially resembling fetal hepatocytes. It is intriguing why mature hepatocytes dedifferentiate into a progenitor state in response to pregnancy. AFP is considered to be produced primarily from fetal liver and thus is used to evaluate fetal development health.
A potential clinical relevance of our data is that we identified maternal liver as a new source of AFP. The hippo signaling pathway has been shown to potently control liver growth and hepatocyte heterogenicity. Surprisingly, we found that pregnancy neither altered the expression nor activities of the components of this pathway and its effector YAP1/TAZ. This finding indicates that pregnancy-induced maternal liver growth is not driven by hippo-YAP1 pathway. However, we demonstrate that the presence of YAP1 is essential for CD133 protein expression in maternal hepatocytes. Collectively, we revealed that, as pregnancy advances, maternal hepatocytes likely undergo endoreplication and display developmental phenotypes. Mechanistically, YAP1 dictates the expression of CD133, contributing to the pregnancy-dependent phenotypic changes of maternal hepatocytes.Lehrer, Helaina. "Investigating the role of the RNA binding protein TDP-43 in Amyotrophic Lateral Sclerosis using animal and cell-based models of disease." Thesis, 2015. https://doi.org/10.7916/D8G44PJQ.
Full text"Vitellogenesis in the red swamp crayfish, Procambarus clarkii." Tulane University, 1998.
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Brown, Margarita. "Investigating the Balance Between Estrogen Receptor Mediated Cell Proliferation and Genomic Surveillance." 2016. https://scholarworks.umass.edu/masters_theses_2/410.
Full text"Mechanisms of tumor necrosis factor-alpha-induced insulin resistance in obesity." Tulane University, 1999.
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"Roles of leptin and the leptin receptor in placental endocrinology and angiogenesis during primate pregnancy." Tulane University, 2001.
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(7022153), Hayly Michelle Goebel. "Characterization of BAF155 and BAF170 in Early Porcine Embryogenesis." Thesis, 2019.
Find full textThe production of developmentally competent in vitro derived embryos is necessary to decreasing both economic and emotional losses. Epigenetic abnormalities/insults have been shown to occur at a higher incidence in in vitro embryos. An increased prevalence of epigenetic derived disorders such as Parkinson’s disease, Prader-Willi syndrome, and α-thalassemia as well as elevated preimplantation embryo arrest and reduced developmental rates are theorized to be caused by errors in the mediation of chromatin remodeling. Chromatin remodeling refers to the restructuring of packaged DNA so that transcription factors are either given more or less access to specific sequences. This can be done by covalent modification through histone methylation, acetylation, and phosphorylation as well as noncovalent modifications which employ ATP dependent chromatin remodeling complexes. The purpose of this thesis was to characterize two structurally integral core subunits, BAF155 and BAF170, of the SWI/SNF chromatin remodeling complex in porcine oocytes and preimplantation embryos.
The first study concentrated on the transcript abundance of BAF155 and BAF170 in porcine oocytes and embryos. First, BAF155 and BAF170 transcript sequences were identified in porcine muscle and heart tissues. Those sequences were used to create quantitative polymerase chain reaction (qPCR) primers. mRNA from pools of GV oocytes (100-800) was converted to cDNA for transcript abundance measurements. However, transcript abundance remained too low for either BAF155 or BAF170 to be accurately quantified.
The second study focused on developmental competency of embryos post interfering RNA (RNAi) knockdown of BAF155, BAF170, or both BAF155/BAF170 combined. After 7 days of culture, an analysis of variance (ANOVA) was performed to determine differences in mean nuclei numbers and morphological blastocyst percentages across the three groups. No significant difference was seen between means of treatment groups vs. both control groups. Significant differences were seen between siRNA and Non-Injected groups as well as Non-Injected and Scramble RNA groups. However this indicates that loss of BAF155, BAF170, or a combination of the two transcripts is not the driving force of the significant differences, rather the microinjection itself caused the differences.
The third study examined the process by which BAF155 and BAF170 proteins are imported from the cytoplasm into the nucleus. It was hypothesized that karyopherin α 7 (KPNA7), a nuclear importer known to be prevalent in the porcine oocyte and early embryo, is the main importer of both subunits. A dominant-negative KPNA7 construct missing the importin beta binding (IBB) domain was microinjected into parthenogenetically activated embryos to outcompete competent wild-type KPNA7. No change in protein localization was seen at the 4-cell stage of development (48 hours post-injection) for either BAF155 or BAF170. To reinforce these results, an RNAi targeting KPNA7 was also microinjected into parthenogenetically activated embryos. Again, no change was shown in protein localization at the 4-cell stage (48 hours post-injection), indicating that KPNA7 was not the main nuclear importer of either BAF155 or BAF170.
Further study is necessary to determine transcript abundance and the mechanism of nuclear import of both BAF155 and BAF170.
Mehregan, Aujan. "Characterization of Calcium Homeostasis Parameters in TRPV3 and CaV3.2 Double Null Mice." 2017. https://scholarworks.umass.edu/masters_theses_2/596.
Full text(9182993), Gabriel L. Curtis. "GENETIC ANALYSIS OF PUTATIVE WALLEYE AND SAUGEYE IN RIVERS NEAR FORT WAYNE, INDIANA." Thesis, 2020.
Find full textA saugeye is the progeny of a female walleye (Sander vitreus) and male sauger (Sander canadensis). In the United States, hybrid saugeyes are considered important for recreational fisheries and as a potential food source. Saugeyes grow exceptionally faster than their non-hybrid parents and are more tolerant of a broader range of water conditions. They are also of interest to anglers due to their increased growth rate and ease to catch. Rather unexpectedly, biologists have recently observed fish that they believe to be saugeye in the Fort Wayne Rivers even though only walleye have been stocked in the area. The fish in Hurshtown Reservoir are believed to be walleye and the identification of those in the Three Rivers is unknown. A potential source for saugeye in the Fort Wayne Rivers is St. Marys State Fish Hatchery in Ohio. This research aims to determine if the fish found in the Fort Wayne Rivers are walleye or saugeye using microsatellite analysis. Microsatellites at seven loci were genotyped for 20 reference walleye, sauger, and saugeye as well as 21 unknown fish caught near Fort Wayne. Of the fish caught near Fort Wayne, three are from Hurshtown Reservoir and 18 are from the Three Rivers. Assignment tests of genotypes were completed using model and non-model based cluster analysis. Genotypic variation clearly resolved the two parent species from their hybrid offspring. Sixteen of eighteen Sander (unknown species) caught in Fort Wayne Rivers between 2018 and 2019 were determined to be first generation saugeye. The other two were walleye found in the Maumee River downstream of Hosey Dam. The three Sander caught in Hurshtown Reservoir were verified to be walleye. Sauger have never been stocked in the Fort Wayne Rivers and connecting waterways. Therefore, it is not likely that the saugeye found in the analysis are from natural reproduction. It is speculated that saugeye are swimming to Fort Wayne from hatcheries within the Maumee watershed. There are many potential sources for walleye in the Fort Wayne Rivers.
(11022450), Jonathan Mark LaCombe. "DYRK1A-RELATED TRABECULAR DEFECTS IN MALE TS65DN MICE EMERGE DURING A CRITICAL DEVELOPMENTAL WINDOW." Thesis, 2021.
Find full textDown syndrome (DS) is a complex genetic disorder caused by the triplication of human chromosome 21 (Hsa21). The presence of an extra copy of an entire chromosome greatly disrupts the copy number and expression of over 350 protein coding genes. This gene dosage imbalance has far-reaching effects on normal development and aging, leading to cognitive and skeletal defects that emerge earlier in life than the general population.
The present study begins by characterizing skeletal development in young male Ts65Dn mice to test the hypothesis that skeletal defects in male Ts65Dn mice are developmental in nature.Femurs from young mice ranging from postnatal day 12- to 42-days of age (P12-42) were measured and analyzed by microcomputed tomography (μCT). Cortical defects were present generally throughout development, but trabecular defects emerged at P30 and persisted until P42.
The gene Dual-specificity tyrosine-regulated kinase 1a (Dyrk1a) is triplicated in both DS and in Ts65Dn mice and has been implicated as a putative cause of both cognitive and skeletal defects. To test the hypothesis that trisomic Dyrk1a is related to the emergence of trabecular defects at P30, expression of Dyrk1a in the femurs of male Ts65Dn mice was quantified by qPCR. Expression was shown to fluctuate throughout development and overexpression generally aligned with the emergence of trabecular defects at P30.
The growth rate in trabecular measures between male Ts65Dn and euploid littermates was similar between P30 and P42, suggesting a closer look into cellular mechanisms at P42. Assessment of proliferation of BMSCs, differentiation and activity of osteoblasts showed no significant differences between Ts65Dn and euploid cellular activity, suggesting that the cellular microenvironment has a greater influence on cellular activity than genetic background.
These data led to the hypothesis that reduction of Dyrk1a gene expression and pharmacological inhibition of DYRK1A could be executed during a critical period to prevent the emergence of trabecular defects at P30. To tests this hypothesis, doxycycline-induced cre-lox recombination to reduce Dyrk1a gene copy number or the DYRK1A inhibitor CX-4945 began at P21. The results of both genetic and pharmacological interventions suggest that trisomic Dyrk1a does not influence the emergence of trabecular defects up to P30. Instead, data suggest that the critical window for the rescue of trabecular defects lies between P30 and P42.(7023215), Carlie Nicole Priddy. "Mechanotransduction in Living Bone: Effects of the Keap1-Nrf2 Pathway." Thesis, 2019.
Find full text(6618536), Stefanie J. Simpson. "TRPV4 Implications in Inflammation and Hydrocephalic Neurological Disease." Thesis, 2019.
Find full text(8703303), Andrew J. Boria. "MRI-TRACKABLE MURINE MODEL OF CEREBRAL RADIATION NECROSIS." Thesis, 2020.
Find full textCerebral radiation necrosis as a consequence of radiation therapy is often observed in patients several months to years after treatment. Complications include painful headaches, seizures, and in the worst-case death. Radiation necrosis is an irreversible condition with the options available to manage it all having noticeable downsides. As such, there is a critical need for better ways of either preventing the onset of necrosis and/or managing its symptoms. As radiation necrosis cannot be induced in humans for ethical reasons, a mouse model that mirrors the features of radiation necrosis observed in patients would allow for new techniques to be tested before being used in human clinical trials. This thesis will explain how our lab designed a murine model of cerebral radiation necrosis that uses a 320 keV cabinet irradiator to produce radiation necrosis and MRI and histology to evaluate the development of radiation necrosis at multiple time points.
Our model required the development of a mouse positioning apparatus that could be used in the cabinet irradiator used as well as the machining of lead shields so that focal semi-hemispheric irradiations could be conducted with other critical structures spared. The MRI scans used as well as the algorithm used to draw radiation necrosis lesions were based off what has been used in previous Gamma Knife models of radiation necrosis. Our initial work showed that since the cabinet irradiator has a relatively flat dose distribution unlike the Gamma Knife, the radiation lesion volumes produced in the former either plateaued or decreased, unlike in the case of the latter where lesion volumes tended to decrease over time. Further work analyzed the effects of fractionation and found minimal sparing using four different fractionation schemes. The effects of strain and sex on the development of radiation necrosis were also analyzed, with strain being found to be a statistically significant parameter while sex was not. Future research should focus on testing the effects of new drugs and techniques for better dealing with radiation necrosis.
(11198013), Kevin Wee. "Creation, deconstruction, and evaluation of a biochemistry animation about the role of the actin cytoskeleton in cell motility." Thesis, 2021.
Find full textExternal representations (ERs) used in science education are multimodal ensembles consisting of design elements to convey educational meanings to the audience. As an example of a dynamic ER, an animation presenting its content features (i.e., scientific concepts) via varying the feature’s depiction over time. A production team invited the dissertation author to inspect their creation of a biochemistry animation about the role of the actin cytoskeleton in cell motility and the animation’s implication on learning. To address this, the author developed a four-step methodology entitled the Multimodal Variation Analysis of Dynamic External Representations (MVADER) that deconstructs the animation’s content and design to inspect how each content feature is conveyed via the animation’s design elements.
This dissertation research investigated the actin animation’s educational value and the MVADER’s utility in animation evaluation. The research design was guided by descriptive case study methodology and an integrated framework consisting of the variation theory, multimodal analysis, and visual analytics. As stated above, the animation was analyzed using MVADER. The development of the actin animation and the content features the production team members intended to convey via the animation were studied by analyzing the communication records between the members, observing the team meetings, and interviewing the members individually. Furthermore, students’ learning experiences from watching the animation were examined via semi-structured interviews coupled with post- storyboarding. Moreover, the instructions of MVADER and its applications in studying the actin animation were reviewed to determine the MVADER’s usefulness as an animation evaluation tool.
Findings of this research indicate that the three educators in the production team intended the actin animation to convey forty-three content features to the undergraduate biology students. At least 50% of the student who participated in this thesis learned thirty-five of these forty-three (> 80%) features. Evidence suggests that the animation’s effectiveness to convey its features was associated with the features’ depiction time, the number of identified design elements applied to depict the features, and the features’ variation of depiction over time.
Additionally, one-third of the student participants made similar mistakes regarding two content features after watching the actin animation: the F-actin elongation and the F-actin crosslink structure in lamellipodia. The analysis reveals the animation’s potential design flaws that might have contributed to these common misconceptions. Furthermore, two disruptors to the creation process and the educational value of the actin animation were identified: the vagueness of the learning goals and the designer’s placement of the animation’s beauty over its reach to the learning goals. The vagueness of the learning goals hampered the narration scripting process. On the other hand, the designer’s prioritization of the animation’s aesthetic led to the inclusion of a “beauty shot” in the animation that caused students’ confusion.
MVADER was used to examine the content, design, and their relationships in the actin animation at multiple aspects and granularities. The result of MVADER was compared with the students’ learning outcomes from watching the animation to identify the characteristics of content’s depiction that were constructive and disruptive to learning. These findings led to several practical recommendations to teach using the actin animation and create educational ERs.
To conclude, this dissertation discloses the connections between the creation process, the content and design, and the educational implication of a biochemistry animation. It also introduces MVADER as a novel ER analysis tool to the education research and visualization communities. MVADER can be applied in various formats of static and dynamic ERs and beyond the disciplines of biology and chemistry.