Relevant bibliographies by topics / 060602 Animal Physiology - Cell

Academic literature on the topic '060602 Animal Physiology - Cell'

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Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "060602 Animal Physiology - Cell"

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Razani, B. "Caveolae: From Cell Biology to Animal Physiology." Pharmacological Reviews 54, no. 3 (September 1, 2002): 431–67. http://dx.doi.org/10.1124/pr.54.3.431.

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RUNSTADLER, PETER W. "The Importance of Cell Physiology to the Performance of Animal Cell Bioreactors." Annals of the New York Academy of Sciences 665, no. 1 Biochemical E (October 1992): 380–90. http://dx.doi.org/10.1111/j.1749-6632.1992.tb42601.x.

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Akers, R. M. "Lactation physiology: A ruminant animal perspective." Protoplasma 159, no. 2-3 (June 1990): 96–111. http://dx.doi.org/10.1007/bf01322593.

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Koretsky, A. P. "Investigation of cell physiology in the animal using transgenic technology." American Journal of Physiology-Cell Physiology 262, no. 2 (February 1, 1992): C261—C275. http://dx.doi.org/10.1152/ajpcell.1992.262.2.c261.

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Over the past 10 years significant progress has been made in techniques for manipulating the genome of the animal. Production of transgenic mice has led to important insights into the regulation of gene expression, the molecular basis of cancer, immunology, and developmental biology. The tools necessary to generate transgenic mice are becoming widely available, making it possible to study a variety of problems. In this review a description of the strategies being used to address problems of interest in cell physiology using transgenic mice is given. Elucidation of the rules governing the regulation of gene expression now permits the targeted expression of a protein to a particular organ or cell type within an organ. Overexpression of proteins, expression of foreign or mutant proteins, mislocalization of proteins, and directed elimination of proteins are all procedures that can now be used to generate interesting animal models for physiological studies. The applications of these techniques to a variety of problems in normal and abnormal physiology are discussed in this review.
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Aravalli, Rajagopal N., Clifford J. Steer, M. Behnan Sahin, and Erik N. K. Cressman. "Stem Cell Origins and Animal Models of Hepatocellular Carcinoma." Digestive Diseases and Sciences 55, no. 5 (June 10, 2009): 1241–50. http://dx.doi.org/10.1007/s10620-009-0861-x.

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Oth, Guirad, and Yrick John. "The Discovery and History of Animal and Human Physiology." Journal La Lifesci 1, no. 6 (December 31, 2020): 19–27. http://dx.doi.org/10.37899/journallalifesci.v1i6.289.

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This study discusses Nucleus, history of the discovery of the cell nucleus Structure and parts of the cell nucleus. All of them is the phisiology of animal and human. The cell nucleus (nucleus) can be defined as an organelle found in eukaryotic cells. Nucleoplasm The nucleoplasm is the liquid that is in the nucleus which is thick and transparent. The cell nucleus has many genes from DNA which are arranged and form structures called chromosomes. The endoplasmic reticulum consists of tubules, vesicles and flattened pockets that occupy the cytoplasmic space. The endoplasmic reticulum is a part of the cell that consists of a membrane system, which has a structure that resembles a multi-layered sac. These sacs are called cisternae.
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Barbe, Michael T., Hannah Monyer, and Roberto Bruzzone. "Cell-Cell Communication Beyond Connexins: The Pannexin Channels." Physiology 21, no. 2 (April 2006): 103–14. http://dx.doi.org/10.1152/physiol.00048.2005.

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Direct cell-to-cell communication through specialized intercellular channels is a characteristic feature of virtually all multi-cellular organisms. The remarkable functional conservation of cell-to-cell coupling throughout the animal kingdom, however, is not matched at the molecular level of the structural protein components. Thus protostomes (including nematodes and flies) and deuterostomes (including all vertebrates) utilize two unrelated families of gap-junction genes, innexins and connexins, respectively. The recent discovery that pannexins, a novel group of proteins expressed by several organisms, are able to form intercellular channels has started a quest to understand their evolutionary relationship and functional contribution to cell communication in vivo. There are three pannexin genes in mammals, two of which are co-expressed in the developing and adult brain. Of note, pannexin1 can also form Ca2+-activated hemichannels that open at physiological extracellular Ca2+ concentrations and exhibit distinct pharmacological properties.
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Leulier, François, Lesley T. MacNeil, Won-jae Lee, John F. Rawls, Patrice D. Cani, Martin Schwarzer, Liping Zhao, and Stephen J. Simpson. "Integrative Physiology: At the Crossroads of Nutrition, Microbiota, Animal Physiology, and Human Health." Cell Metabolism 25, no. 3 (March 2017): 522–34. http://dx.doi.org/10.1016/j.cmet.2017.02.001.

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Rasmussen, Leif, Hans Toftlund, and Peter Suhr-Jessen. "Utilization of iron complexes in an animal cell." Journal of Cellular Physiology 122, no. 1 (January 1985): 155–58. http://dx.doi.org/10.1002/jcp.1041220123.

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Lang, M. A. "Correlation between osmoregulation and cell volume regulation." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 252, no. 4 (April 1, 1987): R768—R773. http://dx.doi.org/10.1152/ajpregu.1987.252.4.r768.

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The euryhaline crab, Callinectes sapidus, behaves both as an osmoregulator when equilibrated in salines in the range of 800 mosM and below and an osmoconformer when equilibrated in salines above 800 mosM. There exists a close correlation between osmoregulation seen in the whole animal in vivo and cell volume regulation studied in vitro. Hyperregulation of the hemolymph osmotic pressure and cell volume regulation both occurred in salines at approximately 800 mosM and below. During long-term equilibration of the crabs to a wide range of saline environments, the total concentration of hemolymph amino acids plus taurine remained below 3 mM. During the first 6 h after an acute osmotic stress to the whole animal, the hemolymph osmotic pressure and Na activity gradually decreased, whereas the free amino acids remained below 3 mM. As the hemolymph osmotic pressure decreased below approximately 850 mosM, the amino acid level began to increase to 17-25 mM. This change was primarily due to increases in glycine, proline, taurine, and alanine. The likely source of the increase in hemolymph free amino acids in vivo is the free amino acid loss from muscle cells observed during cell volume regulation in vitro.
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Dissertations / Theses on the topic "060602 Animal Physiology - Cell"

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Silverman, David A. "Red blood cell spacing in capillaries of rat heart." Thesis, University of Ottawa (Canada), 1996. http://hdl.handle.net/10393/9668.

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Theoretical studies have demonstrated a pronounced effect of red blood cell (RBC) spacing on tissue oxygen supply. In spite of this, realistic values for RBC spacing and related capillary hematocrit (Hct) are not known in the heart. One of the possible reasons is the lack of proper methodology. Thus, the goal of this research study was twofold: (i) to develop a method to rapidly freeze rat heart in situ, following which RBCs and capillary walls could be simultaneously visualized, and (ii) to apply this methodology in rat hearts to establish whether differences exist in capillary Hct and RBC spacing in two distinct locations within the capillary bed, in the subendo- and midmyocardium, during diastole or systole. The results of this study suggest that different geometrical conditions (e.g. RBC spacing and capillary Hct) exist at the two distinct locations of the capillary bed studied. Presumably, the oxygen supply conditions in distal portions of the capillary bed are improved by these geometrical adjustments, preventing hypoxic or anoxic conditions in the tissue, which would be detrimental to cardiac function. (Abstract shortened by UMI.)
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Bindon, Shawn. "Interrelationship between chloride cell proliferation and gas transfer in the rainbow trout." Thesis, University of Ottawa (Canada), 1994. http://hdl.handle.net/10393/6797.

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This thesis examines the morphological and physiological changes that occur in the gills of the rainbow trout, Oncorhynchus mykiss, as a result of chloride cell proliferation. In the first component of this thesis (Chapter 2), the effects of chloride cell hyperplasia and hypertrophy on ion transport capability and the morphological diffusing capacity of the gill were evaluated. These experiments were performed to test the hypothesis that chloride cell proliferation benefits ionic regulation at the expense of efficient gas transfer. Concomitant with the surface morphology results, significant increases in Na$\sp+$ (J$\rm\sb{in}Na\sp+)$ and Cl$\sp-$ uptake (J$\rm\sb{in}Cl\sp-)$ were observed following treatment with the individual combined hormone regimens. Conversely, the gas morphological diffusion capacity, as indicated by the morphological parameters measured, of hormone treated fish was reduced, and was inversely correlated to chloride cell fractional surface area. Surface morphometric analysis showed that the hormone treatment increased the average chloride cell surface area by 2.7 x and chloride cell density by 2.2 x; the combined effect was a five-fold increase in chloride cell fractional area. While the P$\rm\sb aO\sb2$ values of hormone-treated and control fish were similar at P$\rm\sb wO\sb2>12.0$ kPa, the arterial O$\sb2$ tensions of treated fish were significantly lower than those of the control group for P$\rm\sb wO\sb2\leq12.0$ kPa. In comparison, to control fish at all environmental O$\sb2$ tensions, the hormone-treated fish exhibited elevated P$\rm\sb aCO\sb2$ values and a significant acidosis. The effects of chloride cell proliferation on blood gas parameters in hormone-treated fish were accompanied by significantly elevated ventilation amplitudes and lowered ventilation frequency. (Abstract shortened by UMI.)
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Zhang, Hui 1971. "The role of Rho GTPases in complement-mediated glomerular epithelial cell injury /." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98529.

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In glomerular epithelial cells (GEC), the actin cytoskeleton is a key determinant of cell morphology and functions, including permselectivity. Complement C5b-9 induces sublytic GEC injury associated with GEC morphological changes and proteinuria. This study addressed the role of Rho GTPases in complement-mediated GEC injury. We demonstrated that the amount of active RhoA increased; while the amount of active Rac1 and Cdc42 were decreased in C5b-9 mediated sublytic GEC injury both in vitro and in glomeruli from rats with PHN in vivo. Complement mediated inactivation of p190RhoGAP may contribute to complement-induced RhoA activation. Overexpression of constitutively active or dominant negative mutants of RhoA, Rac1 and Cdc42 distinctly altered GEC morphology and F-actin pattern. Complement caused changes in GEC actin cytoskeleton, at least in part mediated by a downstream kinase of RhoA--Rho kinase (ROCK). Activation of RhoA exacerbated complement-mediated cytotoxicity in GEC, while inhibition of ROCK attenuated it.
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Sohi, Jasloveleen. "Investigation of factors regulating parathyroid cell proliferation." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55530.

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Parathyroid glands are responsible for maintaining normal extracellular calcium concentrations through their release of PTH. Calcium and 1,25-(OH)$ rm sb2D sb3$ have been demonstrated to be potent regulators of PTH and CgA synthesis and release. Primary cultures of quiescent bovine parathyroid cells proliferate in response to high concentrations of serum. Next, I examined the role of c-myc in the proliferation of the PT-r cell line, which was cloned from rat parathyroid cells. In order to study the role of c-myc in PT-r cell proliferation more precisely, I used antisense RNA technology to inhibit c-myc mRNA.
In summary, my studies have shown that parathyroid cells respond to selected growth factors. This proliferative response involves increased expression of c-myc, c-fos, c-jun and PTHrP. 1,25-(OH)$ rm sb2D sb3$ inhibits the expression or c-myc, and cell proliferation is inhited. The differentiated parathyroid cell expresses high levels of CgA and PTH. However, during proliferation these high levels are not sustained.
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Sayegh, Camil E. "The role of the B cell receptor complex in avian B cell development dissected by retroviruses /." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=37621.

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During embryogenesis, B cell precursors that have undergone productive Ig V(D)J rearrangement are selected to expand in oligoclonal follicles of the bursa of Fabricius. Because Ig V(D)J recombination in chickens results in minimal diversity, diversity being generated instead by gene conversion in the follicles of the bursa of Fabricius, B cell precursors express a limited range of Ig specificities prior to colonization of the bursa. It has been proposed that recognition of endogenous ligands by this 'pre-diversified' B cell receptor is critical to the progression of normal B cell development. To test this hypothesis, we constructed a truncated IgM receptor (Tmu) lacking the V and Cmu1 domain, which does not associate with Ig L chain proteins nor does it require IgL chains for surface expression, and used a retroviral gene transfer system to introduce it in developing chick embryos. In these embryos, Tmu+ B cell precursors productively colonized bursal follicles as efficiently as B cell precursors expressing endogenous sIg. Furthermore, we detected low but significant levels of IgL VJ rearrangements in Tmu + bursal cells. The analysis of these VJ junctions revealed no selection for in-frame products as these cells are maintained by the Tmu receptor. Interestingly, we showed that the rearranged VL segments derived from Tmu+ bursal cells underwent gene conversion indistinguishably from rearranged VL segments derived from bursal cells expressing endogenous sIg. Taken together, we have ruled out a role for V(D)J encoded determinants in the normal development of B cells in avian embryos. Sequence analysis of 80 IgL VJ segments derived from Tmu+ bursal allowed the unique opportunity to assess the efficiency of gene conversion in vivo, in the absence of selection. Using this system, we demonstrated that >97% of gene conversion events maintain the sequence in-frame. Following hatching, the bursa undergoes morphological changes initiated by the migration of bursal cells ba
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Komorowski, Joanna Irena. "Influence of protein kinase C activators and inhibitors on rat granulosa cell steroidogenesis in vitro." Thesis, University of Ottawa (Canada), 1993. http://hdl.handle.net/10393/6745.

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The present studies were undertaken to determine the involvement of protein kinase C (PKC) in the regulation of rat granulosa cell steroidogenesis in vitro. The effects of PKC activators (1-oleoyl-2-acetylglycerol (OAG); 1,2-dioctanoylglycerol (DiC$\sb8$) and phorbol 12-myristate 13-acetate (TPA)) and inhibitors (DL-Sphingosine (ESP) and 1-(5-Isoquinolinylsulfonyl)-3-methylpiperazine free base (H$\sb7)\rbrack$ on basal and FSH-, (Bu)$\sb2$cAMP-, forskolin- and calcium ionophore A23187-stimulated pregnenolone (P$\sb5$), progesterone (P) and 20$\alpha$-hydroxy-pregn-4-en-3-one (20$\alpha$-OH-P) secretion by granulosa cells were studied. OAG, when continually present in the culture medium (MEM), significantly stimulated P$\sb5$, P and 20$\alpha$-OH-P secretion during 6 to 24 h culture periods. It also markedly increased the conversion of exogenous P$\sb5$ to P and 20$\alpha$-OH-P and exogenous P to 20$\alpha$-OH-P during 24 h cultures. Pretreatment of granulosa cells with TPA for 1 h or treatment for up to 6 h resulted in a significant increase in P$\sb5$, P and 20$\alpha$-OH-P secretion. Except for 20$\alpha$-OH-P production, which was stimulated by the phorbol ester during all culture periods studied, secretion of P$\sb5$ and P (in the presence or absence of exogenous hormones and the inhibitors of steroidogenic enzymes) were substantially inhibited by TPA during a 24 h incubation. However, when granulosa cells were incubated with both OAG (20 $\mu$g/ml) and TPA (40 ng/ml), progestin secretion was increased irrespective of the duration of incubation. PKC inhibitors dose-dependently suppressed the stimulatory effect of OAG (20 $\mu$g/ml) and TPA (40 ng/ml) with complete inhibition noted at 100 $\mu$M of H$\sb7$ and 10 $\mu$M of ESP. Diacylglycerols and TPA exerted divergent effects on FSH-, (Bu)$\sb2$cAMP- and forskolin-stimulated progestin secretion. FSH-stimulated accumulation of P$\sb5$ throughout the culture periods (1-24 h) was markedly increased by OAG (20 $\mu$g/ml) but inhibited by TPA (40 ng/ml). OAG (5-80 $\mu$g/ml) and DiC$\sb8$ (20 $\mu$g/ml) significantly enhanced FSH-induced progestin secretion during 6 h and 24 h culture periods and increased steroid synthesis in 24 h cultures in the presence of (Bu)$\sb2$cAMP or forskolin. In contrast, TPA significantly inhibited FSH- and (Bu)$\sb2$cAMP-stimulated progestin secretion during both 6 h and 24 h of incubation. Pretreatment of granulosa cells with TPA (40 ng/ml) for 20 h to down-regulate PKC, decreased progestin secretion during subsequent incubation with FSH (150 ng/ml) and prevented any stimulation by OAG (20 $\mu$g/ml). The effects of OAG (20 $\mu$g/ml) and TPA (40 ng/ml) on FSH-induced steroid secretion appeared to be additive when both PKC activators were present together and differed significantly from those when OAG and TPA were present with FSH separately. Diolein (a nonpermeable diacylglycerol), 4$\alpha$-phorbol 12,13-didecanoate and phorbol 13-monoacetate (two phorbol esters with no tumor promoting activity) did not influence basal or FSH-stimulated steroid secretion by granulosa cells. (Abstract shortened by UMI.)
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Dubé, Gilles. "Post-translational processing of atrial natriuretic factor. A study using a novel cell culture system." Thesis, University of Ottawa (Canada), 1992. http://hdl.handle.net/10393/7779.

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In the present work, a reproducible cell culture system using adult rat atrial cardiocytes was developed to study ANF processing. Freshly isolated atrial cardiocytes stored high molecular weight ANF (38.0 $\pm$ 4.5 pg/$\mu$g of DNA) and released almost exclusively (83.3% $\pm$ 6.7%) low molecular weight ANF, at an average rate of 12 pg/hour/$\mu$g of DNA. The cell content and the rate of release of ANF decreased over 15 days in culture to 3.9 $\pm$ 1.2 pg/$\mu$g of DNA and 0.32 pg/h/$\mu$g of DNA $\pm$ 0.08 respectively and 62.7% $\pm$ 6.3% of the released peptide was of a low molecular weight. Cultures of non-cardiocytes, superfused with exogenous proANF, did not process the peptide. There was no correlation between the changes in cell population and the reduction in processing. Therefore, atrial non-cardiocytes are not involved in ANF processing. The results presented in this work vary from other reports which found that ANF processing in cultures is absent. The discrepancies may be due to differences related to serum-free culture conditions versus serum supplemented cultures. This suggests that factors present in the serum may be responsible for maintaining ANF processing activity in culture. (Abstract shortened by UMI.)
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Pietersen, Alexander Nicolaas Johannes. "Adenosinergic modulation of hippocampal gamma oscillations : from single cell to whole animal." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/1041/.

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Gamma oscillations, synchronous network activity between 30 and 100 Hz, have been linked to higher cognitive functions. Adenosine receptor modulation has been shown to alter cognitive function in animals and humans. In this thesis the effects of adenosine receptor modulation on in vitro and in vivo hippocampal gamma oscillations were investigated as well as the underlying mechanisms. \(A_1\)-receptor activation selectively decreased gamma oscillations while blocking \(A_1\)-receptors and activating \(A_{2A}\)-receptors increased gamma oscillations. Increasing endogenous adenosine levels suppressed gamma oscillations while decreasing endogenous adenosine levels facilitated gamma oscillations in vitro. Sharp electrode current clamp and whole-cell voltage clamp experiments showed that \(A_1\)-receptor activation hyperpolarised resting membrane potential, reduced firing rate and EPSP amplitude and shifted the IPSC reversal potential to more negative potentials. Blocking \(A_1\)-receptors increased pyramidal cell excitability and increased excitatory synaptic transmission. The results in vivo were more ambiguous but \(A_1\)-receptor activation decreased power in all frequency bands indicating that adenosine receptors can modulate hippocampal gamma oscillations in vivo. \(A_1\)-receptor blockage had no consistent effect on in vivo hippocampal gamma oscillations. Adenosine receptors modulate gamma oscillations in rodent hippocampal slices but are difficult targets for developing treatments that have cognitive benefits because of their ambiguous effects in vivo.
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Dhawan, Anil. "The role of oocyte- and embryo-secreted factors in cumulus cell differentiation and their relationship to embryo quality and developmental competence." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0028/MQ52295.pdf.

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Kim, Changsung. "Assessing the function of Caenorhabditis elegans Ror receptor tyrosine kinase CAM-1 in cell migration, cell polarity, and axon protrusion." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3215192.

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Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2006.
Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1801. Adviser: Wayne C. Forrester. "Title from dissertation home page (viewed June 20, 2007)."
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Books on the topic "060602 Animal Physiology - Cell"

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M, Clynes, ed. Animal cell culture techniques. Berlin: Springer, 1998.

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Mello, Walmor C. Cell-to-Cell Communication. Boston, MA: Springer US, 1987.

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Elson, Elliot S. Cell Membranes: Methods and Reviews Volume 3. Boston, MA: Springer US, 1987.

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Denker, Hans-Werner. Trophoblast Invasion and Endometrial Receptivity: Novel Aspects of the Cell Biology of Embryo Implantation. Boston, MA: Springer US, 1990.

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Kaspar, Anna. How plant and animal cells differ. New York: Britannica Educational Publishing, in association with Rosen Educational Services, 2015.

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European Society of Animal Cell Technology., International Association of Biological Standardization., Fondazione iniziative zooprofilattiche (Brescia, Italy), and Fondazione internazionale Menarini, eds. Proceedings of the Joint ESACT/IABS Meeting on the Production and Exploitation of Existing and New Animal Cell Substrates, held at Villa Alba, Gardone Riviera, Italy, 21-25 May 1984. Basel: S. Karger, 1985.

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Filaretova, L. P. (Li︠u︡dmila Pavlovna) and Takeuchi K. (Koji), eds. Cell/tissue injury and cytoprotection/organoprotection in the gastrointestinal tract: Mechanisms, prevention, and treatment. Basel: Karger, 2012.

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International Workshop on Adenosine and Xanthine Derivatives (1984 Wiesbaden, Germany). Adenosine: Receptors and modulation of cell function : proceedings of the International Workshop on Adenosine and Xanthine derivatives. Oxford [Oxfordshire]: IRL Press, 1985.

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Culture of animal cells: A manual of basic technique and specialized applications. 6th ed. Hoboken, N.J: John Wiley & Sons, 2010.

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Rőszer, Tamás. The Biology of Subcellular Nitric Oxide. Dordrecht: Springer Netherlands, 2012.

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Book chapters on the topic "060602 Animal Physiology - Cell"

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Carroll, Silvia, Mariam Naeiri, and Mohamed Al-Rubeai. "Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry." In Animal Cell Biotechnology, 223–37. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-399-8_9.

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Aspinall, V., M. Cappello, and C. Phillips. "Principles of cell biology." In Introduction to animal and veterinary anatomy and physiology, 3–15. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789241150.0003.

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Sidler, F., T. Wermelinger, L. Rist, A. Hensel, and A. Viviani. "Influence of Mistletoe Extracts and Its Components on in Vitro Physiology of Cancer Cells." In Animal Cell Technology Meets Genomics, 183–85. Dordrecht: Springer Netherlands, 2005. http://dx.doi.org/10.1007/1-4020-3103-3_36.

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Takuma, Shinya, Chikashi Hirashima, and James M. Piret. "Effects of Glucose and CO2 Concentrations on CHO Cell Physiology." In Animal Cell Technology: Basic & Applied Aspects, 99–103. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0726-8_17.

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Schmelzer, Albert E., William M. Miller, Vivian M. Dezengotita, and Lisa R. Abston. "Environmental Effects on Cell Physiology and Metabolism: Response to Elevated pC02." In Animal Cell Technology: From Target to Market, 121–28. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0369-8_27.

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Ikonomou, Laertis, Jean-Christophe Drugmand, G. Bastin, Yves-Jacques Schneider, and Spiros N. Agathos. "Physiology of Insect Cells Cultured in a New Serum-Free Medium." In Animal Cell Technology: From Target to Market, 338–40. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0369-8_77.

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Ridley, Alison, Jonathan Dempsey, Chris Gee, Richard Turner, Matthew Osborne, Steve Ruddock, Christy Ritchie, and Ray Field. "Impact of Change in Fermentation Process pH and Dissolved Carbon Dioxide Concentration on Secreted Antibody Structure and Cell Physiology of a GS-NS0 Cell Line Expressing a Human Antibody." In Animal Cell Technology Meets Genomics, 637–40. Dordrecht: Springer Netherlands, 2005. http://dx.doi.org/10.1007/1-4020-3103-3_129.

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May, Tobias, Milada Butueva, Sara Bantner, Herbert Weich, Hansjörg Hauser, and Dagmar Wirth. "Human Endothelial Cell Lines with In Vivo Physiology." In Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009, 225–34. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0884-6_34.

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Bernal, Vicente, Nuno Carinhas, Francisca Monteiro, Raquel Ambrósio, Manuel J. T. Carrondo, and Paula M. Alves. "An Insight into the Physiology of Insect Cells: The Role of Energetic Metabolism on the Cell Density Effect." In Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009, 299–305. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0884-6_45.

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"5.1 Physiology and Metabolism of Animal Cells for Production." In Animal Cell Biotechnology, 301–25. De Gruyter, 2014. http://dx.doi.org/10.1515/9783110278965.301.

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Conference papers on the topic "060602 Animal Physiology - Cell"

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Dreher, Rachel, Ryan Power, and Binil Starly. "Biofabrication of Multi-Material 3D Neural Constructs Embedded With Patterned PC12 Neural Cell Lines." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14249.

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In Vitro models are being used as a bridge between animal and human studies. Being able to reproduce specific tissue-like structures, functions and responses in a way that is more physiologically relevant allows for huge advantages for tissue engineering, pharmaco-toxicology and food research. These systems are not designed to be directly implanted into patients, but can be used to study human tissue physiology and pathophysiology in vitro. In vitro models are based on human cells, which can capture the responses of the human body, particularly those that are species specific. Models of tissues and organs can give enhanced predictive power, particularly for large-scale screening assays and to understand complex disease pathology.
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2

Marshall, Lauren, Andra Frost, Tim Fee, and Joel Berry. "Assembly and Characterization of 3D, Vascularized Breast Cancer Tissue Mimics." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14199.

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Drug development platforms such as two-dimensional (2D) in vitro cell culture systems and in vivo animal studies do not accurately predict human in vivo effectiveness of candidate therapeutics [1]. Cell culture systems have limited similarities to primary human cells and tissues as only one cell type is employed and animal studies have a generally limited ability to recapitulate human drug response as different species have differences in metabolism, physiology, and behavior. Mike Leavitt, a former U.S. Secretary of Health and Human Services, has stated that “currently, nine out of ten experimental drugs fail in clinical studies because we cannot accurately predict how they will behave in people based on laboratory and animal studies” [2]. Therefore, this research project is focused on developing an in vitro platform to test candidate therapeutics for more efficacious predictions of human response. We have fabricated a three-dimensional (3D) breast cancer tissue volume containing a vascular network. This vascular network is necessary because current in vitro systems (e.g., rotating bioreactors, suspension of spheroids, and growth on a porous scaffold) are limited in size (1–2 mm) by their absence of micrometer-scale blood flow micro-channels that allow for oxygen and nutrient diffusion into the tissue [4]. The extracellular matrix scaffold has been developed to mimic the native extracellular matrix and includes relevant cell types (e.g., human breast cancer epithelial cells and human breast fibroblasts) along with the prefabricated vascular network (prevascularization). These systems are intended to support long-term growth, recapitulate physiological tissue function, and accurately model response to treatment. It is hypothesized that the development of reproducible tissue volumes will transform breast cancer drug development by providing reliable, cost-effective models that can more accurately predict therapeutic efficacy than current preclinical in vivo and in vitro models.
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Milivojević, Nevena, David Caballero, Mariana Carvalho, Marko Živanović, Nenad Filipovic, Rui Reis, and Joaquim Oliveira. "ENGINEERING A MICROFLUDIC PLATFORM AS A PRE-CLINICAL MODEL FOR BIOMEDICAL APPLICATIONS." In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac,, 2021. http://dx.doi.org/10.46793/iccbi21.259m.

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Further technological advances are in great need for improving our understanding about critical biological and fundamental pathological processes, such as tissue development and cancer progression, or for the discovery and screening of novel pharmacological drugs. Preclinical experimentation demands for highly reliable and physiologically-relevant systems capable of recapitulating the complex human physiology. Traditional in vitro models, albeit widely employed, fail to reproduce the complexity of the native scenario with cells displaying aberrant gene expressions. Similarly, in vivo animal models, such as mice, poorly mimic the human condition and are ethically questionable. During the last decades, a new paradigm in preclinical modelling has emerged aiming to solve the limitations of the aforementioned methods. The combination of advanced tissue engineering, nanotechnology, and cell biology has resulted in the development of cutting-edge microfluidics-based models with an unprecedented ability to recreate within a microfluidic device the native habitat of cells within a microengineered chip. A diverse variety of micro- and bio-fabrication techniques is available for the development of microfluidic devices. Among all them, UV-photolithography and soft lithography is the considered the gold-standard method for the fabrication of chips due to its simplicity, versatility, and rapid prototyping. In this work, we describe the step-by-step fabrication procedure of a microfluidic chip by UV-photolithography and replica molding and discuss about their potential applications in the biomedical field.
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