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Journal articles on the topic '030405 Molecular Medicine'

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Author: Grafiati

Published: 10 December 2022

Last updated: 28 January 2023

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1

Li, Chunyan, Lin Zhang, Guangping Meng, Qi Wang, Xuejiao Lv, Jie Zhang, and Junyao Li. "Circular RNAs: pivotal molecular regulators and novel diagnostic and prognostic biomarkers in non-small cell lung cancer." Journal of Cancer Research and Clinical Oncology 145, no. 12 (October 19, 2019): 2875–89. http://dx.doi.org/10.1007/s00432-019-03045-4.

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Saber, Mohamed, El-Sayed M. El El-Kenawy, Abdelhameed Ibrahim, Marwa M. Eid, and Abdelaziz A. Abdelhamid. "New Optimization Models for Sine Cosine Functions in Embedded Telecommunication Systems." International Journal of Wireless and Ad Hoc Communication 3, no. 2 (2021): 102–9. http://dx.doi.org/10.54216/ijwac.030205.

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Trigonometric functions are essential part of digital communication systems such as receivers, synthesizers, and phase locked loop. Implementation of trigonometric functions requires many arithmetic units; multipliers and adders circuits which reduces the speed of operation and consumes much power. In this paper we introduce two approximation methods to represents sine, and cosine functions to achieve fast operation and low power consumption. The simulations indicate a matching between the ideal trigonometric functions and the approximation method with 0.001 error which considered as trivial amount.

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Xiao, Dian, Fei Xie, Xin Xu, and Xinbo Zhou. "The impact and mechanism of ampakine CX1739 on protection against respiratory depression in rats." Future Medicinal Chemistry 12, no. 23 (December 2020): 2093–104. http://dx.doi.org/10.4155/fmc-2020-0256.

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Background: Abuse of analgesic and sedative drugs often leads to severe respiratory depression and sometimes death. Approximately 69,000 people worldwide die annually from opioid overdoses. Purpose: This work aimed to investigate whether CX1739 can be used for emergency treatment of acute respiratory depression due to drug abuse. Results: First, the results clarify that CX1739 is a low-impact ampakine that can safely activate α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors without causing excito-neurotoxicity. Second, CX1739 rapidly crossed the blood–brain barrier (Tmax = 2 min), which meets the requirement of rapid onset of action in vivo. Our work provides preliminarily confirmation that high-dose intravenous administration of CX1739 can immediately reverse respiratory depression in animal models of respiratory depression caused by opioid agonist 030418, pentobarbital sodium and ethanol.

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.., Abedallah Z., and Rasha Almajed. "Deep Neural Network-based Fusion and Natural Language Processing in Additive Manufacturing for Customer Satisfaction." Fusion: Practice and Applications 3, no. 1 (2021): 70–90. http://dx.doi.org/10.54216/fpa.030105.

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Modern Machine learning fusion approaches tend to extract features depending on two techniques (hand-crafted feature and representation learning). Hand-crafted features can waste time and are not sufficient for downstream tasks. Unlike representation learning, we automatically learn features with minimum time and effort and are suitable for downstream tasks. In our paper, we provide work on graph neural network methods with details on classical graph embedding approaches and the different methods in neural graph networks such as graph filtering, graph pooling, and the learning parameter for graph following each technique with a general framework or mathematical proof for customer satisfaction. To satisfy customer's feel, this research employs NLP techniques. We describe the adversarial attacks and defenses on graph representation approaches. Also, advanced application of neural graph networks is reviewed, such as combinational optimization, learning program representation, physical system modeling, and natural language processing. Finally, the challenges in geometric neural networks and future research work have been introduced.

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Zhu, Jiayi, Yan Wan, Hexiang Xu, Yulang Wu, Bo Hu, and Huijuan Jin. "The role of endogenous tissue-type plasminogen activator in neuronal survival after ischemic stroke: friend or foe?" Cellular and Molecular Life Sciences 76, no. 8 (January 17, 2019): 1489–506. http://dx.doi.org/10.1007/s00018-019-03005-8.

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Dhiman, Kunal, Kaj Blennow, Henrik Zetterberg, Ralph N. Martins, and Veer Bala Gupta. "Cerebrospinal fluid biomarkers for understanding multiple aspects of Alzheimer’s disease pathogenesis." Cellular and Molecular Life Sciences 76, no. 10 (February 15, 2019): 1833–63. http://dx.doi.org/10.1007/s00018-019-03040-5.

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Huang, Dengjing, Jianqiang Huo, Jing Zhang, Chunlei Wang, Bo Wang, Hua Fang, and Weibiao Liao. "Protein S-nitrosylation in programmed cell death in plants." Cellular and Molecular Life Sciences 76, no. 10 (February 19, 2019): 1877–87. http://dx.doi.org/10.1007/s00018-019-03045-0.

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Barros, L. Felipe, Alejandro San Martín, Iván Ruminot, Pamela Y. Sandoval, Felipe Baeza-Lehnert, Robinson Arce-Molina, Daniela Rauseo, Yasna Contreras-Baeza, Alex Galaz, and Sharin Valdivia. "Fluid Brain Glycolysis: Limits, Speed, Location, Moonlighting, and the Fates of Glycogen and Lactate." Neurochemical Research 45, no. 6 (March 6, 2020): 1328–34. http://dx.doi.org/10.1007/s11064-020-03005-2.

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Zhang, Xuefen, Ying Liu, Bo Yang, and Haibo Xu. "Inactivation of the Ventral Pallidum by GABAA Receptor Agonist Promotes Non-rapid Eye Movement Sleep in Rats." Neurochemical Research 45, no. 8 (May 4, 2020): 1791–801. http://dx.doi.org/10.1007/s11064-020-03040-z.

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Beart, Philip M. "Special Issue in Honour of Michael B Robinson." Neurochemical Research 45, no. 6 (May 16, 2020): 1245–46. http://dx.doi.org/10.1007/s11064-020-03045-8.

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Stanciu-Pop, Claudia, Marie-Cécile Nollevaux, Martine Berlière, Francois P. Duhoux, Latifa Fellah, Christine Galant, and Mieke R. Van Bockstal. "Morphological intratumor heterogeneity in ductal carcinoma in situ of the breast." Virchows Archiv 479, no. 1 (January 27, 2021): 33–43. http://dx.doi.org/10.1007/s00428-021-03040-6.

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de la Fouchardiere, Arnaud, Willeke Blokx, Léon C. van Kempen, Boštjan Luzar, Sophie Piperno-Neumann, Susana Puig, Llucia Alos, Eduardo Calonje, and Daniela Massi. "ESP, EORTC, and EURACAN Expert Opinion: practical recommendations for the pathological diagnosis and clinical management of intermediate melanocytic tumors and rare related melanoma variants." Virchows Archiv 479, no. 1 (January 12, 2021): 3–11. http://dx.doi.org/10.1007/s00428-020-03005-1.

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Pantanowitz, Liron, Rohit Mehra, and L. Priya Kunju. "AI reality check when evaluating difficult to grade prostate cancers." Virchows Archiv 478, no. 4 (February 2, 2021): 617–18. http://dx.doi.org/10.1007/s00428-021-03045-1.

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Matsumoto, Takeshi, Takashi Komori, Yuta Yoshino, Tadaaki Ioroi, Tsukasa Kitahashi, Hiromu Kitahara, Kohei Ono, et al. "A Liposomal Gemcitabine, FF-10832, Improves Plasma Stability, Tumor Targeting, and Antitumor Efficacy of Gemcitabine in Pancreatic Cancer Xenograft Models." Pharmaceutical Research 38, no. 6 (May 7, 2021): 1093–106. http://dx.doi.org/10.1007/s11095-021-03045-5.

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Abstract Purpose The clinical application of gemcitabine (GEM) is limited by its pharmacokinetic properties. The aim of this study was to characterize the stability in circulating plasma, tumor targeting, and payload release of liposome-encapsulated GEM, FF-10832. Methods Antitumor activity was assessed in xenograft mouse models of human pancreatic cancer. The pharmacokinetics of GEM and its active metabolite dFdCTP were also evaluated. Results In mice with Capan-1 tumors, the dose-normalized areas under the curve (AUCs) after FF-10832 administration in plasma and tumor were 672 and 1047 times higher, respectively, than after using unencapsulated GEM. The tumor-to-bone marrow AUC ratio of dFdCTP was approximately eight times higher after FF-10832 administration than after GEM administration. These results indicated that liposomal encapsulation produced long-term stability in circulating plasma and tumor-selective targeting of GEM. In mice with Capan-1, SUIT-2, and BxPC-3 tumors, FF-10832 had better antitumor activity and tolerability than GEM. Internalization of FF-10832 in tumor-associated macrophages (TAMs) was revealed by flow cytometry and confocal laser scanning microscopy, and GEM was efficiently released from isolated macrophages of mice treated with FF-10832. These results suggest that TAMs are one of the potential reservoirs of GEM in tumors. Conclusion This study found that FF-10832 had favorable pharmacokinetic properties. The liposomal formulation was more effective and tolerable than unencapsulated GEM in mouse xenograft tumor models. Hence, FF-10832 is a promising candidate for the treatment of pancreatic cancer.

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Desai, Tanvi J., Bahanu Habulihaz, Joe R. Cannon, Arun Chandramohan, Hung Yi Kristal Kaan, Ahmad Sadruddin, Tsz Ying Yuen, et al. "Liposome Click Membrane Permeability Assay for Identifying Permeable Peptides." Pharmaceutical Research 38, no. 5 (March 15, 2021): 843–50. http://dx.doi.org/10.1007/s11095-021-03005-z.

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Wang, Hui-Zhong, Ying-Chun Yan, Min Gou, Yue Yi, Zi-Yuan Xia, Masaru K. Nobu, Takashi Narihiro, and Yue-Qin Tang. "Response of Propionate-Degrading Methanogenic Microbial Communities to Inhibitory Conditions." Applied Biochemistry and Biotechnology 189, no. 1 (April 10, 2019): 233–48. http://dx.doi.org/10.1007/s12010-019-03005-1.

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Mitsui, Ryosuke, Ryosuke Yamada, and Hiroyasu Ogino. "Improved Stress Tolerance of Saccharomyces cerevisiae by CRISPR-Cas-Mediated Genome Evolution." Applied Biochemistry and Biotechnology 189, no. 3 (May 23, 2019): 810–21. http://dx.doi.org/10.1007/s12010-019-03040-y.

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Carballo-Sánchez, Marco P., Miquel Gimeno, George M. Hall, María Gisela Ríos-Durán, and Keiko Shirai. "The Radical-Scavenging Activity of a Purified and Sequenced Peptide from Lactic Acid Fermentation of Thunnus albacares By-Products." Applied Biochemistry and Biotechnology 189, no. 4 (June 4, 2019): 1084–95. http://dx.doi.org/10.1007/s12010-019-03045-7.

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Li, Zengqiang, Zhihui Du, Jie Li, and Yanming Sun. "Comparative analysis on lung transcriptome of Mycoplasma ovipneumoniae (Mo) - infected Bashbay sheep and argali hybrid sheep." BMC Veterinary Research 17, no. 1 (October 13, 2021). http://dx.doi.org/10.1186/s12917-021-03040-3.

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Abstract Background Bashbay sheep (Bbs) has a certain degree of resistance to Mycoplasma ovipneumoniae (Mo), however, Argali hybrid sheep (Ahs) is susceptible to Mo. To understand the molecular mechanisms underlying the difference of the susceptibility for Mo infection, RNA-sequencing technology was used to compare the transcriptomic response of the lung tissue of Mo-infected Bbs and Ahs. Results Six Bbs and six Ahs were divided into experimental group and control group respectively, all of them were experimentally infected with Mo by intratracheal injection. For collecting lung tissue samples, three Bbs and three Ahs were sacrificed on day 4 post-infection, and the others were sacrificed on day 14 post-infection. Total RNA extracted from lung tissue were used for transcriptome analyses based on high-throughput sequencing technique and bioinformatics. The results showed that 212 (146 up-regulated, 66 down-regulated) DEGs were found when comparing transcriptomic data of Bbs and Ahs at 4th dpi, besides, 311 (158 up-regulated, 153 down-regulated) DEGs were found at 14th dpi. After GO analysis, three main GO items protein glycosylation, immune response and positive regulation of gene expression were found related to Mo infection. In addition, there were 20 DEGs enriched in these above items, such as SPLUC1 (BPIFA1), P2X7R, DQA, HO-1 and SP-A (SFTPA-1). Conclusions These selected 20 DEGs associated with Mo infection laid the foundation for further study on the underlying molecular mechanism involved in high level of resistance to Mo expressed by Bbs, meanwhile, provided deeper understandings about the development of pathogenicity and host-pathogen interactions.

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Drugowitsch, Jan, Gregory C. DeAngelis, Eliana M. Klier, Dora E. Angelaki, and Alexandre Pouget. "Optimal multisensory decision-making in a reaction-time task." eLife 3 (June 14, 2014). http://dx.doi.org/10.7554/elife.03005.

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Humans and animals can integrate sensory evidence from various sources to make decisions in a statistically near-optimal manner, provided that the stimulus presentation time is fixed across trials. Little is known about whether optimality is preserved when subjects can choose when to make a decision (reaction-time task), nor when sensory inputs have time-varying reliability. Using a reaction-time version of a visual/vestibular heading discrimination task, we show that behavior is clearly sub-optimal when quantified with traditional optimality metrics that ignore reaction times. We created a computational model that accumulates evidence optimally across both cues and time, and trades off accuracy with decision speed. This model quantitatively explains subjects's choices and reaction times, supporting the hypothesis that subjects do, in fact, accumulate evidence optimally over time and across sensory modalities, even when the reaction time is under the subject's control.

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Zhou, Hao, Xun Shen, Chen Yan, Wu Xiong, Zemeng Ma, Zhenggang Tan, Jinwen Wang, et al. "Extracellular vesicles derived from human umbilical cord mesenchymal stem cells alleviate osteoarthritis of the knee in mice model by interacting with METTL3 to reduce m6A of NLRP3 in macrophage." Stem Cell Research & Therapy 13, no. 1 (July 16, 2022). http://dx.doi.org/10.1186/s13287-022-03005-9.

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Abstract Background Osteoarthritis (OA) is a prevalent degenerative joint disease that not only significantly impairs the quality of life of middle-aged and elderly individuals but also imposes a significant financial burden on patients and society. Due to their significant biological properties, extracellular vesicles (EVs) have steadily received great attention in OA treatment. This study aimed to investigate the influence of EVs on chondrocyte proliferation, migration, and apoptosis and their protective efficacy against OA in mice. Methods The protective impact of EVs derived from human umbilical cord mesenchymal stem cells (hucMSCs-EVs) on OA in mice was investigated by establishing a mouse OA model by surgically destabilizing the medial meniscus (DMM). Human chondrocytes were isolated from the cartilage of patients undergoing total knee arthroplasty (TKA) and cultured with THP-1 cells to mimic the in vivo inflammatory environment. Levels of inflammatory factors were then determined in different groups, and the impacts of EVs on chondrocyte proliferation, migration, apoptosis, and cartilage extracellular matrix (ECM) metabolism were explored. N6-methyladenosine (m6A) level of mRNA and methyltransferase-like 3 (METTL3) protein expression in the cells was also measured in addition to microRNA analysis to elucidate the molecular mechanism of exosomal therapy. Results The results indicated that hucMSCs-EVs slowed OA progression, decreased osteophyte production, increased COL2A1 and Aggrecan expression, and inhibited ADAMTS5 and MMP13 overexpression in the knee joint of mice via decreasing pro-inflammatory factor secretion. The in vitro cell line analysis revealed that EVs enhanced chondrocyte proliferation and migration while inhibiting apoptosis. METTL3 is responsible for these protective effects. Further investigations revealed that EVs decreased the m6A level of NLRP3 mRNA following miR-1208 targeted binding to METTL3, resulting in decreased inflammatory factor release and preventing OA progression. Conclusion This study concluded that hucMSCs-EVs inhibited the secretion of pro-inflammatory factors and the degradation of cartilage ECM after lowering the m6A level of NLRP3 mRNA with miR-1208 targeting combined with METTL3, thereby alleviating OA progression in mice and providing a novel therapy for clinical OA treatment.

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Najafi, Haniyeh, Samira Sadat Abolmaali, Reza Heidari, Hadi Valizadeh, Ali Mohammad Tamaddon, and Negar Azarpira. "Integrin receptor-binding nanofibrous peptide hydrogel for combined mesenchymal stem cell therapy and nitric oxide delivery in renal ischemia/reperfusion injury." Stem Cell Research & Therapy 13, no. 1 (July 26, 2022). http://dx.doi.org/10.1186/s13287-022-03045-1.

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Abstract Background Mesenchymal-based therapy has been utilized as a practical approach in the treatment of renal ischemia/reperfusion (I/R) injury. However, low cell retention and survival in the ischemic site have remained challenging issues. To bridge this gap, the integrin receptor-binding RGD peptide-functionalized, s-nitroso-n-acetyl penicillamine (SNAP)-loaded hydrogel was used to transplant Wharton's jelly-mesenchymal stem cells (WJ-MSCs). Methods Apart from physicochemical and rheological characterizations that confirmed entangled interlocking β-sheets with nanofibrous morphology, real-time RT-PCR, ROS production, serum biomarker concentrations, and histopathological alterations were explored in a mouse model to assess the therapeutic efficacy of formulations in the treatment of renal I/R injury. Results The RGD-functionalized Fmoc-diphenylalanine (Fmoc-FF + Fmoc-RGD) hydrogel supported the spread and proliferation of WJ-MSCs in vivo. Notably, intralesional injection of nitric oxide donor combined with the embedded WJ-MSCs caused superior recovery of renal I/R injury compared to free WJ-MSCs alone in terms of histopathological scores and renal function indices. Compared to the I/R control group, oxidative stress and inducible nitric oxide synthase (iNOS) expression biomarkers showed a significant decline, whereas endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) expression exhibited a significant increment, indicating regeneration of the injured endothelial tissue. Conclusion The findings confirmed that the hydrogels containing WJ-MSCs and nitric oxide donors can promote the regeneration of renal I/R injuries by increasing angiogenic factors and cell engraftment.

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Bejoy, Julie, Justin M. Farry, Jennifer L. Peek, Mariana C. Cabatu, Felisha M. Williams, Richard C. Welch, Eddie S. Qian, and Lauren E. Woodard. "Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease." Stem Cell Research & Therapy 13, no. 1 (July 26, 2022). http://dx.doi.org/10.1186/s13287-022-03040-6.

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Abstract Background In diabetic kidney disease, high glucose damages specialized cells called podocytes that filter blood in the glomerulus. In vitro culture of podocytes is crucial for modeling of diabetic nephropathy and genetic podocytopathies and to complement animal studies. Recently, several methods have been published to derive podocytes from human-induced pluripotent stem cells (iPSCs) by directed differentiation. However, these methods have major variations in media composition and have not been compared. Methods We characterized our accelerated protocol by guiding the cells through differentiation with four different medias into MIXL1+ primitive streak cells with Activin A and CHIR for Wnt activation, intermediate mesoderm PAX8+ cells via increasing the CHIR concentration, nephron progenitors with FGF9 and Heparin for stabilization, and finally into differentiated podocytes with Activin A, BMP-7, VEGF, reduced CHIR, and retinoic acid. The podocyte morphology was characterized by scanning and transmission electron microscopy and by flow cytometry analysis for podocyte markers. To confirm cellular identity and niche localization, we performed cell recombination assays combining iPSC-podocytes with dissociated mouse embryonic kidney cells. Finally, to test iPSC-derived podocytes for the modeling of diabetic kidney disease, human podocytes were exposed to high glucose. Results Podocyte markers were expressed at similar or higher levels for our accelerated protocol as compared to previously published protocols that require longer periods of tissue culture. We confirmed that the human podocytes derived from induced pluripotent stem cells in twelve days integrated into murine glomerular structures formed following seven days of culture of cellular recombinations. We found that the high glucose-treated human podocytes displayed actin rearrangement, increased cytotoxicity, and decreased viability. Conclusions We found that our accelerated 12-day method for the differentiation of podocytes from human-induced pluripotent stem cells yields podocytes with comparable marker expression to longer podocytes. We also demonstrated that podocytes created with this protocol have typical morphology by electron microscopy. The podocytes have utility for diabetes modeling as evidenced by lower viability and increased cytotoxicity when treated with high glucose. We found that multiple, diverse methods may be utilized to create iPSC-podocytes, but closely mimicking developmental cues shortened the time frame required for differentiation.

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Duc, Ha Danh, and Nguyen Thi Oanh. "Composition of bacterial community and isolation of bacteria responsible for diuron degradation in sediment and soil under anaerobic condition." Archives of Microbiology 204, no. 7 (June 23, 2022). http://dx.doi.org/10.1007/s00203-022-03040-3.

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Heydari, Samira, Reza Malekzadeh, Mir Hadi Jazayeri, Abdolfattah Sarrafnejad, and Farideh Siavoshi. "Detection of peptidoglycan in yeast as a marker for the presence or abundance of intracellular Helicobacter pylori and Staphylococcus." Archives of Microbiology 204, no. 7 (June 20, 2022). http://dx.doi.org/10.1007/s00203-022-03045-y.

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Yadav, Mahendra, Vivek Kumar, Nidhi Sandal, and Meenakshi K. Chauhan. "Quantitative evaluation of mercury adsorption and removal efficacy of Spirulina (Arthrospira platensis) powder in mice." Archives of Microbiology 204, no. 7 (June 13, 2022). http://dx.doi.org/10.1007/s00203-022-03005-6.

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Shen, Hong-Yu, Liu-Xi Shi, Lin Wang, Le-Ping Fang, Wei Xu, Ju-Qing Xu, Bo-Qiang Fan, and Wei-Fei Fan. "Hsa_circ_0001361 facilitates the progress of lung adenocarcinoma cells via targeting miR-525-5p/VMA21 axis." Journal of Translational Medicine 19, no. 1 (September 10, 2021). http://dx.doi.org/10.1186/s12967-021-03045-4.

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Abstract Background Lung adenocarcinoma (LUAD) is a common subtype of lung cancer with high recurrence rate and fatality. Circ_0001361 has been recognized as key regulators in various malignancies, but its roles in LUAD remain ambiguous. Methods Circ_0001361, miR-525-5p, and VMA21 levels were assessed by RT-qPCR. The growth and metastasis of LUAD cells were detected by MTT, colony formation, wound scratch, and transwell assays, respectively. The interaction between circ_0001361/VMA21 and miR-525-5p was detected by dual luciferase, RNA immunoprecipitation, and RNA pull-down assays. VMA21 protein level was detected by Western blotting. Nude mouse xenograft model was established to determine the role of circ_0001361 in tumor growth in vivo. Results Circ_0001361 was up-regulated, while miR-525-5p was down-regulated in LUAD tissues and cells. Functional experiments demonstrated that circ_0001361 drove LUAD cell growth and metastasis. Mechanistically, circ_0001361 functioned as a sponge of miR-525-5p to up-regulate downstream target VMA21 level. MiR-525-5p/VMA21 axis was involved in circ_0001361-mediated malignant phenotypes of LUAD cells. Finally, inhibition of circ_0001361 restrained in vivo xenograft tumor growth via regulating miR-525-5p/VMA21 axis. Conclusion Our findings elucidate that circ_0001361 facilitates the tumorigenesis and development of LUAD through miR-525-5p/VMA21 axis, providing evidence for circ_0001361 as a potential prognosis biomarker and therapeutic target for clinical treatment of LUAD.

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Weng, Jie, Ruonan Hou, Xiaoming Zhou, Zhe Xu, Zhiliang Zhou, Peng Wang, Liang Wang, Chan Chen, Jinyu Wu, and Zhiyi Wang. "Development and validation of a score to predict mortality in ICU patients with sepsis: a multicenter retrospective study." Journal of Translational Medicine 19, no. 1 (July 29, 2021). http://dx.doi.org/10.1186/s12967-021-03005-y.

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Abstract Background Early and accurate identification of septic patients at high risk for ICU mortality can help clinicians make optimal clinical decisions and improve the patients’ outcomes. This study aimed to develop and validate (internally and externally) a mortality prediction score for sepsis following admission in the ICU. Methods We extracted data retrospectively regarding adult septic patients from one teaching hospital in Wenzhou, China and a large multi-center critical care database from the USA. Demographic data, vital signs, laboratory values, comorbidities, and clinical outcomes were collected. The primary outcome was ICU mortality. Through multivariable logistic regression, a mortality prediction score for sepsis was developed and validated. Results Four thousand two hundred and thirty six patients in the development cohort and 8359 patients in three validation cohorts. The Prediction of Sepsis Mortality in ICU (POSMI) score included age ≥ 50 years, temperature < 37 °C, Respiratory rate > 35 breaths/min, MAP ≤ 50 mmHg, SpO2 < 90%, albumin ≤ 2 g/dL, bilirubin ≥ 0.8 mg/dL, lactate ≥ 4.2 mmol/L, BUN ≥ 21 mg/dL, mechanical ventilation, hepatic failure and metastatic cancer. In addition, the area under the receiver operating characteristic curve (AUC) for the development cohort was 0.831 (95% CI, 0.813–0.850) while the AUCs ranged from 0.798 to 0.829 in the three validation cohorts. Moreover, the POSMI score had a higher AUC than both the SOFA and APACHE IV scores. Notably, the Hosmer–Lemeshow (H–L) goodness-of-fit test results and calibration curves suggested good calibration in the development and validation cohorts. Additionally, the POSMI score still exhibited excellent discrimination and calibration following sensitivity analysis. With regard to clinical usefulness, the decision curve analysis (DCA) of POSMI showed a higher net benefit than SOFA and APACHE IV in the development cohort. Conclusion POSMI was validated to be an effective tool for predicting mortality in ICU patients with sepsis.

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Mosquera-Giraldo, Laura I., Maria Donoso, Kevin Stefanski, Kimberly Foster, Christoph Gesenberg, Pamela Abraham, Ying Ren, Anne Rose, Chris Freeden, and Asoka Ranasinghe. "Solvent-Casted Films to Assist Polymer Selection for Amorphous Solid Dispersions During Preclinical Studies: In-vitro and In-vivo Exploration." Pharmaceutical Research, April 20, 2021. http://dx.doi.org/10.1007/s11095-021-03040-w.

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Novoyatleva, Tatyana, Nabham Rai, Baktybek Kojonazarov, Swathi Veeroju, Isabel Ben-Batalla, Paola Caruso, Mazen Shihan, et al. "Author Correction: Deficiency of Axl aggravates pulmonary arterial hypertension via BMPR2." Communications Biology 5, no. 1 (January 20, 2022). http://dx.doi.org/10.1038/s42003-022-03045-0.

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Yu, Hong, Yanbin Niu, Guohua Jia, Yujie Liang, Baolin Chen, Ruoyu Sun, Min Wang, et al. "Maternal diabetes-mediated RORA suppression in mice contributes to autism-like offspring through inhibition of aromatase." Communications Biology 5, no. 1 (January 13, 2022). http://dx.doi.org/10.1038/s42003-022-03005-8.

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AbstractRetinoic acid-related orphan receptor alpha (RORA) suppression is associated with autism spectrum disorder (ASD) development, although the mechanism remains unclear. In this study, we aim to investigate the potential effect and mechanisms of RORA suppression on autism-like behavior (ALB) through maternal diabetes-mediated mouse model. Our in vitro study in human neural progenitor cells shows that transient hyperglycemia induces persistent RORA suppression through oxidative stress-mediated epigenetic modifications and subsequent dissociation of octamer-binding transcription factor 3/4 from the RORA promoter, subsequently suppressing the expression of aromatase and superoxide dismutase 2. The in vivo mouse study shows that prenatal RORA deficiency in neuron-specific RORA null mice mimics maternal diabetes-mediated ALB; postnatal RORA expression in the amygdala ameliorates, while postnatal RORA knockdown mimics, maternal diabetes-mediated ALB in offspring. In addition, RORA mRNA levels in peripheral blood mononuclear cells decrease to 34.2% in ASD patients (n = 121) compared to the typically developing group (n = 118), and the related Receiver Operating Characteristic curve shows good sensitivity and specificity with a calculated 84.1% of Area Under the Curve for ASD diagnosis. We conclude that maternal diabetes contributes to ALB in offspring through suppression of RORA and aromatase, RORA expression in PBMC could be a potential marker for ASD screening.

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Lee, Yeonmi, Aysha Trout, Nuria Marti-Gutierrez, Seoon Kang, Philip Xie, Aleksei Mikhalchenko, Bitnara Kim, et al. "Haploidy in somatic cells is induced by mature oocytes in mice." Communications Biology 5, no. 1 (January 25, 2022). http://dx.doi.org/10.1038/s42003-022-03040-5.

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AbstractHaploidy is naturally observed in gametes; however, attempts of experimentally inducing haploidy in somatic cells have not been successful. Here, we demonstrate that the replacement of meiotic spindles in mature metaphases II (MII) arrested oocytes with nuclei of somatic cells in the G0/G1 stage of cell cycle results in the formation of de novo spindles consisting of somatic homologous chromosomes comprising of single chromatids. Fertilization of such oocytes with sperm triggers the extrusion of one set of homologous chromosomes into the pseudo-polar body (PPB), resulting in a zygote with haploid somatic and sperm pronuclei (PN). Upon culture, 18% of somatic-sperm zygotes reach the blastocyst stage, and 16% of them possess heterozygous diploid genomes consisting of somatic haploid and sperm homologs across all chromosomes. We also generate embryonic stem cells and live offspring from somatic-sperm embryos. Our finding may offer an alternative strategy for generating oocytes carrying somatic genomes.

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