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Zeitschriftenartikel zum Thema „Zobellia“

Autor: Grafiati

Veröffentlicht am 25. Mai 2024

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1

Nedashkovskaya, Olga I., Makoto Suzuki, Marc Vancanneyt, Ilse Cleenwerck, Anatoly M. Lysenko, Valery V. Mikhailov und Jean Swings. „Zobellia amurskyensis sp. nov., Zobellia laminariae sp. nov. and Zobellia russellii sp. nov., novel marine bacteria of the family Flavobacteriaceae“. International Journal of Systematic and Evolutionary Microbiology 54, Nr. 5 (01.09.2004): 1643–48. http://dx.doi.org/10.1099/ijs.0.63091-0.

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The taxonomic position of four newly isolated marine, heterotrophic, gliding, Gram-negative, aerobic, pigmented, agarolytic bacteria was established. 16S rRNA gene sequence analysis indicated affiliation of the isolates to the genus Zobellia in the family Flavobacteriaceae. DNA–DNA hybridization experiments revealed that the strains studied represent three distinct and novel species, for which the names Zobellia amurskyensis sp. nov., Zobellia laminariae sp. nov. and Zobellia russellii sp. nov. are proposed, with KMM 3526T (=LMG 22069T=CCUG 47080T), KMM 3676T (=LMG 22070T=CCUG 47083T) and KMM 3677T (=LMG 22071T=CCUG 47084T), respectively, as the type strains.

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Nedashkovskaya, Olga, Nadezhda Otstavnykh, Natalia Zhukova, Konstantin Guzev, Viktoria Chausova, Liudmila Tekutyeva, Valery Mikhailov und Marina Isaeva. „Zobellia barbeyronii sp. nov., a New Member of the Family Flavobacteriaceae, Isolated from Seaweed, and Emended Description of the Species Z. amurskyensis, Z. laminariae, Z. russellii and Z. uliginosa“. Diversity 13, Nr. 11 (22.10.2021): 520. http://dx.doi.org/10.3390/d13110520.

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Six Gram-stain-negative, aerobic, rod-shaped, and motile by gliding bacterial strains were isolated from Pacific green and red algae. Phylogenetic analysis based on 16S rRNA gene sequences placed the novel strains into the genus Zobellia as a distinct evolutionary lineage close to Zobellia nedashkovskayae Asnod2-B07-BT and Zobellia laminariae KMM 3676T sharing the highest similarity of 99.7% and 99.5%, respectively. The average nucleotide identity and the average amino acid identity values between strains 36-CHABK-3-33T and Z. nedashkovskayae Asnod2-B07-BT and Z. laminariae KMM 3676T were 89.7%/92.9% and 94.2%/95.8%, respectively. The digital DNA–DNA hybridization values based on the draft genomes between strains 36-CHABK-3-33T and Z. nedashovskayae Asnod2-B07-BT and Z. laminariae KMM 3676T were 39.5 ± 2.5% and 59.6 ± 2.7%, respectively. Multilocus sequence analysis based on house-keeping genes (dnaK, gyrB, pyrH, recA and topA) assigned the alga-associated isolates to the same species, which clustered separately from the recognized species of the genus Zobellia. The strains under study grew at 4–32 °C and with 0.5–8% NaCl and decomposed aesculin, gelatin, DNA, and Tweens 20 and 80, and weakly agar. The DNA G+C content was 36.7% calculated from genome sequence analysis for the strain 36-CHABK-3-33T. The predominant fatty acids of strain 36-CHABK-3-33T (>5% of the total fatty acids) were iso-C17:0 3-OH, summed feature 3 (comprising C16:1ω7c and/or iso-C15:0 2-OH fatty acids), iso-C15:0, iso-C15:1 G, and C15:0. The major polar lipids were phosphatidylethanolamine, three unidentified lipids, and two unidentified aminolipids. The only detected respiratory quinone was MK-6. The significant molecular distinctiveness between the novel isolates and their nearest neighbor was strongly supported by differences in physiological and biochemical tests. Therefore, the six novel strains represent a novel species of the genus Zobellia, for which the name Zobellia barbeyronii sp. nov. is proposed. The type strain is 36-CHABK-3-33T (= KACC 21790T = KMM 6746T).

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Chernysheva, Nadezhda, Evgeniya Bystritskaya, Galina Likhatskaya, Olga Nedashkovskaya und Marina Isaeva. „Genome-Wide Analysis of PL7 Alginate Lyases in the Genus Zobellia“. Molecules 26, Nr. 8 (20.04.2021): 2387. http://dx.doi.org/10.3390/molecules26082387.

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We carried out a detailed investigation of PL7 alginate lyases across the Zobellia genus. The main findings were obtained using the methods of comparative genomics and spatial structure modeling, as well as a phylogenomic approach. Initially, in order to elucidate the alginolytic potential of Zobellia, we calculated the content of polysaccharide lyase (PL) genes in each genome. The genus-specific PLs were PL1, PL6, PL7 (the most abundant), PL14, PL17, and PL40. We revealed that PL7 belongs to subfamilies 3, 5, and 6. They may be involved in local and horizontal gene transfer and gene duplication processes. Most likely, an individual evolution of PL7 genes promotes the genetic variability of the Alginate Utilization System across Zobellia. Apparently, the PL7 alginate lyases may acquire a sub-functionalization due to diversification between in-paralogs.

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Chernysheva, Nadezhda, Evgeniya Bystritskaya, Anna Stenkova, Ilya Golovkin, Olga Nedashkovskaya und Marina Isaeva. „Comparative Genomics and CAZyme Genome Repertoires of Marine Zobellia amurskyensis KMM 3526T and Zobellia laminariae KMM 3676T“. Marine Drugs 17, Nr. 12 (24.11.2019): 661. http://dx.doi.org/10.3390/md17120661.

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We obtained two novel draft genomes of type Zobellia strains with estimated genome sizes of 5.14 Mb for Z. amurskyensis KMM 3526Т and 5.16 Mb for Z. laminariae KMM 3676Т. Comparative genomic analysis has been carried out between obtained and known genomes of Zobellia representatives. The pan-genome of Zobellia genus is composed of 4853 orthologous clusters and the core genome was estimated at 2963 clusters. The genus CAZome was represented by 775 GHs classified into 62 families, 297 GTs of 16 families, 100 PLs of 13 families, 112 CEs of 13 families, 186 CBMs of 18 families and 42 AAs of six families. A closer inspection of the carbohydrate-active enzyme (CAZyme) genomic repertoires revealed members of new putative subfamilies of GH16 and GH117, which can be biotechnologically promising for production of oligosaccharides and rare monomers with different bioactivities. We analyzed AA3s, among them putative FAD-dependent glycoside oxidoreductases (FAD-GOs) being of particular interest as promising biocatalysts for glycoside deglycosylation in food and pharmaceutical industries.

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Barbeyron, T., S. L'Haridon, E. Corre, B. Kloareg und P. Potin. „Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov.“ International Journal of Systematic and Evolutionary Microbiology 51, Nr. 3 (01.05.2001): 985–97. http://dx.doi.org/10.1099/00207713-51-3-985.

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6

Lucena, Teresa, Javier Pascual, Assunta Giordano, Agata Gambacorta, Esperanza Garay, David R. Arahal, M. Carmen Macián und María J. Pujalte. „Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae“. International Journal of Systematic and Evolutionary Microbiology 60, Nr. 12 (01.12.2010): 2871–76. http://dx.doi.org/10.1099/ijs.0.020875-0.

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Strain 7SM30T , an aerobic marine, Gram-negative, heterotrophic and yellow- to orange-pigmented bacterium isolated from seawater from Castellón, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that the isolate represented a novel lineage within the family Flavobacteriaceae. The most closely related genera were Pseudozobellia, Zobellia and Kriegella. Cells of strain 7SM30T were non-motile rods that required sea salts for growth, used a wide variety of carbohydrates as sole carbon and energy sources and, unlike species of the genera Pseudozobellia and Zobellia, did not possess flexirubin-type pigment or hydrolyse agar. Strain 7SM30T contained MK6 as the sole respiratory quinone. Phosphatidylethanolamine (PE) was the only identifiable polar lipid, although other lipids were also detected. The predominant cellular fatty acids were saturated C15 and monounsaturated C15. The DNA G+C content was around 40 mol%. On the basis of extensive phenotypic and phylogenetic comparative analysis, it is concluded that the new strain represents a novel genus and species, for which the name Euzebyella saccharophila gen. nov., sp. nov., is proposed. The type strain of the type species is 7SM30T (=CECT 7477T=KCTC 22655T).

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Groisillier, Agnès, Aurore Labourel, Gurvan Michel und Thierry Tonon. „The Mannitol Utilization System of the Marine Bacterium Zobellia galactanivorans“. Applied and Environmental Microbiology 81, Nr. 5 (29.12.2014): 1799–812. http://dx.doi.org/10.1128/aem.02808-14.

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ABSTRACTMannitol is a polyol that occurs in a wide range of living organisms, where it fulfills different physiological roles. In particular, mannitol can account for as much as 20 to 30% of the dry weight of brown algae and is likely to be an important source of carbon for marine heterotrophic bacteria.Zobellia galactanivorans(Flavobacteriia) is a model for the study of pathways involved in the degradation of seaweed carbohydrates. Annotation of its genome revealed the presence of genes potentially involved in mannitol catabolism, and we describe here the biochemical characterization of a recombinant mannitol-2-dehydrogenase (M2DH) and a fructokinase (FK). Among the observations, the M2DH ofZ. galactanivoranswas active as a monomer, did not require metal ions for catalysis, and featured a narrow substrate specificity. The FK characterized was active on fructose and mannose in the presence of a monocation, preferentially K+. Furthermore, the genes coding for these two proteins were adjacent in the genome and were located directly downstream of three loci likely to encode an ATP binding cassette (ABC) transporter complex, suggesting organization into an operon. Gene expression analysis supported this hypothesis and showed the induction of these five genes after culture ofZ. galactanivoransin the presence of mannitol as the sole source of carbon. This operon for mannitol catabolism was identified in only 6 genomes ofFlavobacteriaceaeamong the 76 publicly available at the time of the analysis. It is not conserved in allBacteroidetes; some species contain a predicted mannitol permease instead of a putative ABC transporter complex upstream of M2DH and FK ortholog genes.

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David, Benoit, Romain Irague, Diane Jouanneau, Franck Daligault, Mirjam Czjzek, Yves-Henri Sanejouand und Charles Tellier. „Internal Water Dynamics Control the Transglycosylation/Hydrolysis Balance in the Agarase (AgaD) of Zobellia galactanivorans“. ACS Catalysis 7, Nr. 5 (11.04.2017): 3357–67. http://dx.doi.org/10.1021/acscatal.7b00348.

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9

Thomas, François, Tristan Barbeyron und Gurvan Michel. „Evaluation of reference genes for real-time quantitative PCR in the marine flavobacterium Zobellia galactanivorans“. Journal of Microbiological Methods 84, Nr. 1 (Januar 2011): 61–66. http://dx.doi.org/10.1016/j.mimet.2010.10.016.

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10

Correc, Gaëlle, Jan-Hendrik Hehemann, Mirjam Czjzek und William Helbert. „Structural analysis of the degradation products of porphyran digested by Zobellia galactanivorans β-porphyranase A“. Carbohydrate Polymers 83, Nr. 1 (Januar 2011): 277–83. http://dx.doi.org/10.1016/j.carbpol.2010.07.060.

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11

Harms, Henrik, Anna Klöckner, Jan Schrör, Michaele Josten, Stefan Kehraus, Max Crüsemann, Wiebke Hanke, Tanja Schneider, Till Schäberle und Gabriele König. „Antimicrobial Dialkylresorcins from Marine-Derived Microorganisms: Insights into Their Mode of Action and Putative Ecological Relevance“. Planta Medica 84, Nr. 18 (10.07.2018): 1363–71. http://dx.doi.org/10.1055/a-0653-7451.

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Abstract Zobellia galactanivorans has been reported as a seaweed-associated or marine-derived species with largely unknown secondary metabolites. The combination of bioinformatic analysis and MS- and bioactivity guided separation led to the isolation of a new antibiotically active dialkylresorcin from the marine bacterium Z. galactanivorans. The antibiotic profile of the new dialkylresorcin zobelliphol (1) was investigated and compared with related and naturally occurring dialkyresorcins (i.e., stemphol (2) and 4-butyl-3,5-dihydroxybenzoic acid (3)) from the marine-derived fungus Stemphylium globuliferum. Bacterial reporter strain assays provided insights into the mode of action of this antibiotic compound class. We identified an interference with bacterial DNA biosynthesis for the dialkylresorcin derivative 1. In addition, the putative biosynthetic gene cluster corresponding to production of 1 was identified and a biosynthetic hypothesis was deduced.

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Lee, Seungwoo. „Improvement in the Catalytic Activity of β-Agarase AgaA from Zobellia galactanivorans by Site-Directed Mutagenesis“. Journal of Microbiology and Biotechnology 21, Nr. 11 (28.11.2011): 1116–22. http://dx.doi.org/10.4014/jmb.1107.07001.

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13

Dudek, Magda, Anissa Dieudonné, Diane Jouanneau, Tatiana Rochat, Gurvan Michel, Benoit Sarels und François Thomas. „Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT“. Nucleic Acids Research 48, Nr. 14 (25.06.2020): 7786–800. http://dx.doi.org/10.1093/nar/gkaa533.

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Abstract Marine flavobacteria possess dedicated Polysaccharide Utilization Loci (PULs) enabling efficient degradation of a variety of algal polysaccharides. The expression of these PULs is tightly controlled by the presence of the substrate, yet details on the regulatory mechanisms are still lacking. The marine flavobacterium Zobellia galactanivorans DsijT digests many algal polysaccharides, including alginate from brown algae. Its complex Alginate Utilization System (AUS) comprises a PUL and several other loci. Here, we showed that the expression of the AUS is strongly and rapidly (<30 min) induced upon addition of alginate, leading to biphasic substrate utilization. Polymeric alginate is first degraded into smaller oligosaccharides that accumulate in the extracellular medium before being assimilated. We found that AusR, a GntR family protein encoded within the PUL, regulates alginate catabolism by repressing the transcription of most AUS genes. Based on our genetic, genomic, transcriptomic and biochemical results, we propose the first model of regulation for a PUL in marine bacteria. AusR binds to promoters of AUS genes via single, double or triple copies of operator. Upon addition of alginate, secreted enzymes expressed at a basal level catalyze the initial breakdown of the polymer. Metabolic intermediates produced during degradation act as effectors of AusR and inhibit the formation of AusR/DNA complexes, thus lifting transcriptional repression.

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Dorival, Jonathan, Sophie Ruppert, Melissa Gunnoo, Adam Orłowski, Maylis Chapelais-Baron, Jérôme Dabin, Aurore Labourel et al. „The laterally acquired GH5 ZgEngAGH5_4 from the marine bacterium Zobellia galactanivorans is dedicated to hemicellulose hydrolysis“. Biochemical Journal 475, Nr. 22 (28.11.2018): 3609–28. http://dx.doi.org/10.1042/bcj20180486.

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Cell walls of marine macroalgae are composed of diverse polysaccharides that provide abundant carbon sources for marine heterotrophic bacteria. Among them, Zobellia galactanivorans is considered as a model for studying algae–bacteria interactions. The degradation of typical algal polysaccharides, such as agars or alginate, has been intensively studied in this model bacterium, but the catabolism of plant-like polysaccharides is essentially uncharacterized. Here, we identify a polysaccharide utilization locus in the genome of Z. galactanivorans, induced by laminarin (β-1,3-glucans), and containing a putative GH5 subfamily 4 (GH5_4) enzyme, currently annotated as a endoglucanase (ZgEngAGH5_4). A phylogenetic analysis indicates that ZgEngAGH5_4 was laterally acquired from an ancestral Actinobacteria. We performed the biochemical and structural characterization of ZgEngAGH5_4 and demonstrated that this GH5 is, in fact, an endo-β-glucanase, most active on mixed-linked glucan (MLG). Although ZgEngAGH5_4 and GH16 lichenases both hydrolyze MLG, these two types of enzymes release different series of oligosaccharides. Structural analyses of ZgEngAGH5_4 reveal that all the amino acid residues involved in the catalytic triad and in the negative glucose-binding subsites are conserved, when compared with the closest relative, the cellulase EngD from Clostridium cellulovorans, and some other GH5s. In contrast, the positive glucose-binding subsites of ZgEngAGH5_4 are different and this could explain the preference for MLG, with respect to cellulose or laminarin. Molecular dynamics computer simulations using different hexaoses reveal that the specificity for MLG occurs through the +1 and +2 subsites of the binding pocket that display the most important differences when compared with the structures of other GH5_4 enzymes.

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Jang, Min-Kyung, Seung Woo Lee, Dong-Geun Lee, Nam-Young Kim, Ki Hwan Yu, Hye Ji Jang, Suhkman Kim, Andre Kim und Sang-Hyeon Lee. „Enhancement of the thermostability of a recombinant β-agarase, AgaB, from Zobellia galactanivorans by random mutagenesis“. Biotechnology Letters 32, Nr. 7 (08.03.2010): 943–49. http://dx.doi.org/10.1007/s10529-010-0237-5.

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Amiri Moghaddam, Jamshid, Huijuan Guo, Karsten Willing, Thomas Wichard und Christine Beemelmanns. „Identification of the new prenyltransferase Ubi-297 from marine bacteria and elucidation of its substrate specificity“. Beilstein Journal of Organic Chemistry 18 (22.06.2022): 722–31. http://dx.doi.org/10.3762/bjoc.18.72.

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Aromatic prenylated metabolites have important biological roles and activities in all living organisms. Compared to their importance in all domains of life, we know relatively little about their substrate scopes and metabolic functions. Here, we describe a new UbiA-like prenyltransferase (Ptase) Ubi-297 encoded in a conserved operon of several bacterial taxa, including marine Flavobacteria and the genus Sacchromonospora. In silico analysis of Ubi-297 homologs indicated that members of this Ptase group are composed of several transmembrane α-helices and carry a conserved and distinct aspartic-rich Mg2+-binding domain. We heterologously produced UbiA-like Ptases from the bacterial genera Maribacter, Zobellia, and Algoriphagus in Escherichia coli. Investigation of their substrate scope uncovered the preferential farnesylation of quinoline derivatives, such as 8-hydroxyquinoline-2-carboxylic acid (8-HQA) and quinaldic acid. The results of this study provide new insights into the abundance and diversity of Ptases in marine Flavobacteria and beyond.

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Naretto, Anaïs, Mathieu Fanuel, David Ropartz, Hélène Rogniaux, Robert Larocque, Mirjam Czjzek, Charles Tellier und Gurvan Michel. „The agar-specific hydrolase ZgAgaC from the marine bacterium Zobellia galactanivorans defines a new GH16 protein subfamily“. Journal of Biological Chemistry 294, Nr. 17 (07.03.2019): 6923–39. http://dx.doi.org/10.1074/jbc.ra118.006609.

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Wilkens, Casper, Manish K. Tiwari, Helen Webb, Murielle Jam, Mirjam Czjzek und Birte Svensson. „Asp271 is critical for substrate interaction with the surface binding site in β-agarase a from Zobellia galactanivorans“. Proteins: Structure, Function, and Bioinformatics 87, Nr. 1 (04.11.2018): 34–40. http://dx.doi.org/10.1002/prot.25614.

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Brunet, Maéva, Nolwen Le Duff, Bernhard M. Fuchs, Rudolf Amann, Tristan Barbeyron und François Thomas. „Specific detection and quantification of the marine flavobacterial genus Zobellia on macroalgae using novel qPCR and CARD-FISH assays“. Systematic and Applied Microbiology 44, Nr. 6 (November 2021): 126269. http://dx.doi.org/10.1016/j.syapm.2021.126269.

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Brunet, Maéva, Nolwen Le Duff, Bernhard M. Fuchs, Rudolf Amann, Tristan Barbeyron und François Thomas. „Specific detection and quantification of the marine flavobacterial genus Zobellia on macroalgae using novel qPCR and CARD-FISH assays“. Systematic and Applied Microbiology 44, Nr. 6 (November 2021): 126269. http://dx.doi.org/10.1016/j.syapm.2021.126269.

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21

Liu, Zhemin, Guiyang Li, Zhaolan Mo und Haijin Mou. „Molecular cloning, characterization, and heterologous expression of a new κ-carrageenase gene from marine bacterium Zobellia sp. ZM-2“. Applied Microbiology and Biotechnology 97, Nr. 23 (03.10.2013): 10057–67. http://dx.doi.org/10.1007/s00253-013-5215-0.

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22

Peng, Chia-Chi, Nils Dormanns, Lars Regestein und Christine Beemelmanns. „Isolation of sulfonosphingolipids from the rosette-inducing bacterium Zobellia uliginosa and evaluation of their rosette-inducing activity“. RSC Advances 13, Nr. 39 (2023): 27520–24. http://dx.doi.org/10.1039/d3ra04314b.

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23

Cao, Shengsheng, Li Li, Benwei Zhu und Zhong Yao. „Biochemical Characterization and Elucidation of the Hybrid Action Mode of a New Psychrophilic and Cold-Tolerant Alginate Lyase for Efficient Preparation of Alginate Oligosaccharides“. Marine Drugs 20, Nr. 8 (05.08.2022): 506. http://dx.doi.org/10.3390/md20080506.

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Alginate lyases with unique biochemical properties have irreplaceable value in food and biotechnology industries. Herein, the first new hybrid action mode Thalassotalea algicola-derived alginate lyase gene (TAPL7A) with both psychrophilic and cold-tolerance was cloned and expressed heterologously in E. coli. With the highest sequence identity (43%) to the exolytic alginate lyase AlyA5 obtained from Zobellia galactanivorans, TAPL7A was identified as a new polysaccharide lyases family 7 (PL7) alginate lyase. TAPL7A has broad substrate tolerance with specific activities of 4186.1 U/mg, 2494.8 U/mg, 2314.9 U/mg for polyM, polyG, and sodium alginate, respectively. Biochemical characterization of TAPL7A showed optimal activity at 15 °C, pH 8.0. Interestingly, TAPL7A exhibits both extreme psychrophilic and cold tolerance, which other cold-adapted alginate lyase do not possess. In a wide range of 5–30 °C, the activity can reach 80–100%, and the residual activity of more than 70% can still be maintained after 1 h of incubation. Product analysis showed that TAPL7A adopts a hybrid endo/exo-mode on all three substrates. FPLC and ESI-MS confirmed that the final products of TAPL7A are oligosaccharides with degrees of polymerization (Dps) of 1–2. This study provides excellent alginate lyase candidates for low-temperature environmental applications in food, agriculture, medicine and other industries.

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Kwon, Kae Kyoung, Yoo Kyung Lee und Hong Kum Lee. „Costertonia aggregata gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from a mature biofilm“. International Journal of Systematic and Evolutionary Microbiology 56, Nr. 6 (01.06.2006): 1349–53. http://dx.doi.org/10.1099/ijs.0.64168-0.

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A marine bacterium, strain KOPRI 13342T, was isolated from a mature marine biofilm, including various marine algae, covering a rock-bed of the East Sea, Korea (also known as the Sea of Japan). Colonies of the isolate were orange-coloured on marine agar 2216. The isolate showed relatively high 16S rRNA gene sequence similarities to members of the genera Maribacter (91.2–92.4 % similarity), Zobellia (90.7–91.5 %) and Muricauda (90.7–91.4 %). Phylogenetic analysis based on the nearly complete 16S rRNA gene sequence revealed that the isolate formed a phyletic lineage with members of the genus Muricauda. Cells were aerobic, motile, Gram-negative rods and they produced non-diffusible carotenoid pigments. Optimal growth was observed at pH 7.5–8.0 and 26–32 °C and required the presence of 3 % (w/v) sea salt. The strain required Ca2+ and K+ ions in addition to NaCl for growth. The dominant fatty acids were i-15 : 0, i-15 : 1ω10, 15 : 0 and 16 : 1ω9. The major respiratory quinone was MK-6. The DNA G+C content was 35.8 mol%. On the basis of this polyphasic taxonomic evidence, strain KOPRI 13342T should be classified as a representative of a novel species in a new genus in the family Flavobacteriaceae; the name Costertonia aggregata gen. nov., sp. nov. is proposed. The type strain of Costertonia aggregata is KOPRI 13342T (=KCCM 42265T=JCM 13411T).

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JAM, Murielle, Didier FLAMENT, Julie ALLOUCH, Philippe POTIN, Laurent THION, Bernard KLOAREG, Mirjam CZJZEK, William HELBERT, Gurvan MICHEL und Tristan BARBEYRON. „The endo-β-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours“. Biochemical Journal 385, Nr. 3 (24.01.2005): 703–13. http://dx.doi.org/10.1042/bj20041044.

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Two β-agarase genes, agaA and agaB, were functionally cloned from the marine bacterium Zobellia galactanivorans. The agaA and agaB genes encode proteins of 539 and 353 amino acids respectively, with theoretical masses of 60 and 40 kDa. These two β-agarases feature homologous catalytic domains belonging to family GH-16. However, AgaA displays a modular architecture, consisting of the catalytic domain (AgaAc) and two C-terminal domains of unknown function which are processed during secretion of the enzyme. In contrast, AgaB is composed of the catalytic module and a signal peptide similar to the N-terminal signature of prokaryotic lipoproteins, suggesting that this protein is anchored in the cytoplasmic membrane. Gel filtration and electrospray MS experiments demonstrate that AgaB is a dimer in solution, while AgaAc is a monomeric protein. AgaAc and AgaB were overexpressed in Escherichia coli and purified to homogeneity. Both enzymes cleave the β-(1→4) linkages of agarose in a random manner and with retention of the anomeric configuration. Although they behave similarly towards liquid agarose, AgaAc is more efficient than AgaB in the degradation of agarose gels. Given these organizational and catalytic differences, we propose that, reminiscent of the agarolytic system of Pseudoalteromonas atlantica, AgaA is specialized in the initial attack on solid-phase agarose, while AgaB is involved with the degradation of agarose fragments.

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Fournier, Jean-Baptiste, Etienne Rebuffet, Ludovic Delage, Romain Grijol, Laurence Meslet-Cladière, Justyna Rzonca, Philippe Potin, Gurvan Michel, Mirjam Czjzek und Catherine Leblanc. „The Vanadium Iodoperoxidase from the Marine Flavobacteriaceae Species Zobellia galactanivorans Reveals Novel Molecular and Evolutionary Features of Halide Specificity in the Vanadium Haloperoxidase Enzyme Family“. Applied and Environmental Microbiology 80, Nr. 24 (26.09.2014): 7561–73. http://dx.doi.org/10.1128/aem.02430-14.

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ABSTRACTVanadium haloperoxidases (VHPO) are key enzymes that oxidize halides and are involved in the biosynthesis of organo-halogens. Until now, only chloroperoxidases (VCPO) and bromoperoxidases (VBPO) have been characterized structurally, mainly from eukaryotic species. Three putative VHPO genes were predicted in the genome of the flavobacteriumZobellia galactanivorans, a marine bacterium associated with macroalgae. In a phylogenetic analysis, these putative bacterial VHPO were closely related to other VHPO from diverse bacterial phyla but clustered independently from eukaryotic algal VBPO and fungal VCPO. Two of these bacterial VHPO, heterogeneously produced inEscherichia coli, were found to be strictly specific for iodide oxidation. The crystal structure of one of these vanadium-dependent iodoperoxidases, Zg-VIPO1, was solved by multiwavelength anomalous diffraction at 1.8 Å, revealing a monomeric structure mainly folded into α-helices. This three-dimensional structure is relatively similar to those of VCPO of the fungusCurvularia inaequalisand ofStreptomycessp. and is superimposable onto the dimeric structure of algal VBPO. Surprisingly, the vanadate binding site of Zg-VIPO1 is strictly conserved with the fungal VCPO active site. Using site-directed mutagenesis, we showed that specific amino acids and the associated hydrogen bonding network around the vanadate center are essential for the catalytic properties and also the iodide specificity of Zg-VIPO1. Altogether, phylogeny and structure-function data support the finding that iodoperoxidase activities evolved independently in bacterial and algal lineages, and this sheds light on the evolution of the VHPO enzyme family.

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Wallace, Michael D., Laura Guée, David Ropartz, Mathieu Fanuel, Guillaume Lannuzel, Gaëlle Correc, Keith A. Stubbs und Elizabeth Ficko-Blean. „Characterisation of an exo-(α-1,3)-3,6-anhydro-d-galactosidase produced by the marine bacterium Zobellia galactanivorans DsijT: Insight into enzyme preference for natural carrageenan oligosaccharides and kinetic characterisation on a novel chromogenic substrate“. International Journal of Biological Macromolecules 163 (November 2020): 1471–79. http://dx.doi.org/10.1016/j.ijbiomac.2020.07.298.

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Huyyirnah, Huyyirnah, und Rosmaniar R. „Modifikasi Medium Menggunakan Saline-Water Soluble Fraction (SSF) atau Fraksi Minyak Terlarut untuk Menumbuhkan Bakteri Pendegradasi Hidrokarbon“. Indonesian Journal of Laboratory 4, Nr. 2 (03.12.2021): 72. http://dx.doi.org/10.22146/ijl.v4i2.69282.

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Isolasi bakteri pendegradasi hidrokarbon memerlukan teknik yang baik dan nutrisi optimal untuk pertumbuhannya. Kendala dalam pembuatan medium dan pengamatan isolat bakteri yang mengandung hidrokarbon sering terjadi di laboratorium, sehingga dibutuhkan teknik pengembangan metode dalam proses isolasi bakteri pendegradasi hidrokarbon. Penelitian ini bertujuan untuk mengetahui dan membandingkan jumlah dan jenis koloni bakteri yang tumbuh dalam medium Zobell+saline-water soluble fraction (SSF) dibandingkan dengan medium Zobell+minyak bumi. Metode penelitian yaitu mengisolasi bakteri menggunakan medium Zobell+SSF 6 jam (A), 12 jam (B), 24 jam (C) dan sebagai kontrol adalah medium Zobell+1% v/v minyak bumi (K), perhitungan bakteri menggunakan metode TPC dan mengidentifikasi bakteri dengan alat VITEK-MS. Hasil penelitian memperlihatkan bahwa jumlah bakteri yang tumbuh pada medium Zobell+SSF 24 jam (C) adalah 6.9 x 108 CFU/ml, hal ini menunjukkan lebih baik dibandingkan dengan Zobell+1% v/v minyak bumi (K)=5.2 x 108 CFU/ml, medium Zobell+SSF 12 jam (B)=6.6 x 107 CFU/ml dan medium Zobell+SSF 6 jam (A)=1.8 x 107 CFU/ml.Kesimpulan penelitian ini adalah bahwa dari segi jumlah total bakteri medium modifikasi Zobell+SSF pengadukan selama 24 jam (C) lebih baik dalam menumbuhkan bakteri pendegradasi hidrokarbon dibandingkan dengan pengadukan 6 jam (A), 12 jam (B), dan medium Zobell+1% v/v minyak bumi (K). Sedangkan apabila berdasarkan dengan keragaman bakteri, didapatkan hasil bahwa strain bakteri yang terisolasi pada medium modifikasi Zobell+SSF perlakuan pengadukan 6, 12, 24 jam sama dengan strain bakteri yang tumbuh pada kontrol (medium Zobell +1% v/v minyak bumi. Bakteri yang teridentifikasi sebagai bakteri pendegaradsi hidrokarbon adalah bakteri Klebsiella pneumoniae, Enterobacter asburiae/Enterobacter cloacae dan Pseudomonas aeruginosa.

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Cunliffe, Michael, Ro Allen und Nathan Chrismas. „The genome sequence of a marine yeast, Metschnikowia zobellii (Uden & Cast.-Branco, 1961)“. Wellcome Open Research 8 (19.09.2023): 411. http://dx.doi.org/10.12688/wellcomeopenres.19998.1.

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We present a genome assembly from a Metschnikowia zobellii culture (a marine yeast; Ascomycota; Saccharomycetes; Saccharomycetales; Metschnikowiaceae). The genome sequence is 13.6 megabases in span. Most of the assembly is scaffolded into 5 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 51.12 kilobases in length.

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Kokoulin, Maxim S., Pavel S. Dmitrenok und Lyudmila A. Romanenko. „Structure of the Lipooligosaccharide from the Deep-Sea Marine Bacterium Idiomarina zobellii KMM 231T, Isolated at a Depth of 4000 Meters“. Marine Drugs 20, Nr. 11 (09.11.2022): 700. http://dx.doi.org/10.3390/md20110700.

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The structural characterization of lipopolysaccharides has critical implications for some biomedical applications, and marine bacteria are an inimitable source of new glyco-structures potentially usable in medicinal chemistry. On the other hand, lipopolysaccharides of marine Gram-negative bacteria present certain structural features that can help the understanding of the adaptation processes. The deep-sea marine Gram-negative bacterium Idiomarina zobellii KMM 231T, isolated from a seawater sample taken at a depth of 4000 m, represents an engaging microorganism to investigate in terms of its cell wall components. Here, we report the structural study of the R-type lipopolysaccharide isolated from I. zobellii KMM 231T that was achieved through a multidisciplinary approach comprising chemical analyses, NMR spectroscopy, and MALDI mass spectrometry. The lipooligosaccharide turned out to be characterized by a novel and unique pentasaccharide skeleton containing a very short mono-phosphorylated core region and comprising terminal neuraminic acid. The lipid A was revealed to be composed of a classical disaccharide backbone decorated by two phosphate groups and acylated by i13:0(3-OH) in amide linkage, i11:0 (3-OH) as primary ester-linked fatty acids, and i11:0 as a secondary acyl chain.

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Monta, RY. „In Memoriam Dr Claude E ZoBell“. Marine Ecology Progress Series 58 (1989): 1–2. http://dx.doi.org/10.3354/meps058001.

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Vanderslice, John. „What Happened Here by Bonnie ZoBell“. Pleiades: Literature in Context 36, Nr. 1S (2016): 37–39. http://dx.doi.org/10.1353/plc.2016.0036.

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Kerkhof, Lee. „A ribosomal RNA operon from Pseudomonas stutzeri Zobell“. Gene 192, Nr. 2 (Juni 1997): 241–43. http://dx.doi.org/10.1016/s0378-1119(97)00081-4.

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Turton, Gillian C., und Alastair C. Wardlaw. „Pathogenicity of the marine yeasts Metschnikowia zobelli and Rhodotorula rubra for the sea urchin Echinus esculentus“. Aquaculture 67, Nr. 1-2 (Dezember 1987): 199–202. http://dx.doi.org/10.1016/0044-8486(87)90027-5.

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ROSSELLO-MORA, R. A., E. GARCIA-VALDeS und J. LALUCAT. „Taxonomic Relationship between Pseudomonas perfectomarina ZoBell and Pseudomonas stutzeri“. International Journal of Systematic Bacteriology 43, Nr. 4 (01.10.1993): 852–54. http://dx.doi.org/10.1099/00207713-43-4-852.

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Cai, Mengli, und Russell Timkovich. „Solution Conformation of Ferricytochrome c-551 fromPseudomonas stutzeriSubstrain ZoBell“. Biochemical and Biophysical Research Communications 254, Nr. 3 (Januar 1999): 675–78. http://dx.doi.org/10.1006/bbrc.1998.9989.

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Nurfajriah, Siti, Maulin Inggraini und Noor Andryan Ilsan. „SKRINNING RHIZOBAKTERI MANGROVE Rhizosphora Sp. PENGHASIL AMILASE“. Jurnal Mitra Kesehatan 1, Nr. 1 (30.12.2018): 11–15. http://dx.doi.org/10.47522/jmk.v1i1.4.

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Pendahuluan: Ekosistem mangrove merupakan ekosistem yang kaya akan nutrisi karena dipengaruhi oleh pasang surut air laut, asupan air tawar dari daratan, akumulasi mineral, dan aktivitas mikroorganisme. Kondisi tersebut menghasilkan ekosistem yang unik dan memiliki keanekaragaman mikroorganisme. Rhizobakteri adalah bakteri yang hidup pada daerah rhizosfer dan membentuk koloni pada sistem perakaran tumbuhan. Rhizobakteri diketahui memiliki bermacam enzim, salah satunya antara lain enzim amilase. Enzim amilase banyak digunakan di industri makanan, tekstil, dam kertas. Metode: Tujuan penelitian ini adalah mengisolasi dan menskrining amilase yang dihasilkan rizhobakteri dari tanaman mangrove dan bakteri serasah pada mangrove Rhizophora Sp. Isolasi bakteri dilakukan dengan seri pengenceran yang ditumbuhkan dalam medium zobell. Skrinning aktivitas amilase dilakukan dengan menumbuhkan bakteri dalam medium zobell agar yang mengandung pati. Hasil: Isolat rhizobakteri yang berhasil diisolasi dari tumbuhan mangrove muda, mangrove tua, dan serasah berjumlah 42 isolat. Hasil skrining menunjukkan 30 isolat mampu menghasilkan a-amilase. Kesimpulan: Berdasarkan penelitian dapat disimpulkan bahwa isolat Rhizobakteri mangrove yang berhasil diisolasi dari akar tanaman mangrove Pulau Bira Kepulauan Seribu sebanyak 42 isolat dan 30 isolat menghasilkan enzim a-amilase. Isolat yang paling banyak menghasilkan enzim tersebut berasal dari rhizobakteri tanaman mangrove muda dan zona bening yang yang terbesar yaitu 7 mm.

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Mendonça-Hagler, Leda Cristina, Allen N. Hagler, H. J. Phaff und Joanne Tredick. „DNA relatedness among aquatic yeasts of the genus Metschnikowia and proposal of the species Metschnikowia australis comb. nov.“ Canadian Journal of Microbiology 31, Nr. 10 (01.10.1985): 905–9. http://dx.doi.org/10.1139/m85-170.

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DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschnikowia bicuspidata var. bicuspidata and of the varieties chathamia and californica. On this basis, low intervarietal fertility, and unique habitat in antarctic seawater, we have proposed to raise M. bicuspidata var. australis to the rank of species, Metschnikowia australis, comb. nov. DNA complementarity of Metschnikowia bicuspidata var. californica and Metschnikowia bicuspidata var. chathamia DNA was greater than 80% with that of M. bicuspidata var. bicuspidata. Metschnikowia australis can be differentiated from other Metschnikowia species and varieties by its inability to form chlamydospores, the formation of two needle-shaped ascospores per ascus, lack of glucose fermentation, and lack of assimilation of both methyl-α-D-glucoside and glucono-δ-lactone. Other DNA–DNA reassociation experiments showed that Metschnikowia zobellii is a distinct species when compared with both aquatic and terrestrial species of the genus.

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Koyongian, Silvia E., Deiske A. Sumilat, Rosita A. J. Lintang, Stenly Wullur, Sandra O. Tilaar und Henneke Pangkey. „ISOLASI BAKTERI YANG BERSIMBION DENGAN ASCIDIAN Herdmania momus YANG MEMILIKI AKTIVITAS ANTIBAKTERI“. JURNAL PESISIR DAN LAUT TROPIS 8, Nr. 2 (30.05.2020): 20. http://dx.doi.org/10.35800/jplt.8.2.2020.28766.

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Ascidian is marine invertebrates in coral reef ecosystems that produce many bioactive compounds for pharmacology. The presence of symbiotic bacteria with marine organisms is protected the host biota by producing secondary metabolites. The purpose of this study is to obtain symbiotic bacterial isolates with Herdmania momus ascidian, then to observe the antibacterial activity of these bacterial isolates against Escherichia coli, and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria were made on Nutrient Agar and Zobell Marine Broth media. The antibacterial screening showed that the Herdmania momus symbiotic bacteria were able to inhibit the growth of Staphylococcus aureus and Escherichia coli.Keywords: ascidians, Herdmania momus, bacteria, isolation, antibacterialAbstak Ascidian adalah avetebrata laut di ekosistem terumbu karang yang banyak menghasilkan senyawa bioaktif untuk bidang farmakologi. Keberadaan bakteri yang bersimbion dengan organisme laut pada umumnya untuk melindungi biota yang ditumpanginya dan dirinya dengan cara menghasilkan senyawa metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mendapatkan isolat bakteri yang bersimbion dengan ascidian Herdmania momus, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli, dan Staphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dengan ascidian dibuat pada media Nutrient Agar dan Zobell Marine Broth. Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan ascidian Herdmania momus mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli.Kata kunci: ascidian, Herdmania momus, bakteri, isolasi, antibakteri

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Ayu, Muhammad Ramli und La Ode Baytul Abidin. „KARAKTERISASI BIOKIMIA DAN IDENTIFIKASI ISOLAT BAKTERI PADA MAKROALGA Padina australis DARI PERAIRAN PANTAI TANJUNG TIRAM“. Jurnal Sapa Laut (Jurnal Ilmu Kelautan) 6, Nr. 1 (09.04.2021): 11. http://dx.doi.org/10.33772/jsl.v6i1.17551.

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Padina australis merupakan salah satu makroalga yang memiliki potensi sebagai antibiotik. Sifat antibiotik yang dimiliki diduga tidak hanya bersumber dari makroalga tersebut namun juga dihasilkan oleh bakteri simbion pada inangnya. Penelitian ini bertujuan untuk mengisolasi dan identifikasi isolat bakteri yang dikultur dari makroalga P. australis berdasarkan pengamatan morfologi bakteri dan karakter biokimia. Sampel makroalga P. australis diambil dari Desa Tanjung Tiram, Kecamatan Moramo Utara, Kabupaten Konawe Selatan menggunakan metode koleksi bebas (free handpacking). Sampel makroalga dihaluskan dan diencerkan hingga 10-5, kultur bakteri dilakukan dari pengenceran sampel 10-4 dan 10-5. Bakteri dikultur dengan menggunakan metode cawan sebar pada media agar zobell selama 2x24 jam pada suhu 35oC. Koloni bakteri yang tumbuh dipurifikasi dengan cara dikultur kembali sebanyak dua kali pengulangan dengan metode gores. Kultur murni bakteri yang diperoleh kemudian dibiakkan pada media agar miring zobell. Isolat bakteri yang tumbuh dari sumber pengenceran sampel 10-4 dan 10-5 selanjutnya dikarakterisasi berdasarkan pengamatan morfologi dan uji biokimia. Kedua isolat bakteri dari makroalga P. australis memiliki ciri morfologi yang sama, namun terdapat perbedaan pada karakteristik biokimia yaitu pada uji motility, OF (oksidatif/fermentatif), TCBS (Thiosulfate Citrate Bilesalt Sucrose), ornitin, gelatin, uji produksi asam pada media berbahan D-manose, maltose, rhammose, urease dan citrase. Berdasarkan identifikasi jenis bakteri yang merujuk pada Bergey’s Manual of Systematic Bacteriology diketahui bahwa isolat bakteri simbion makroalga P. australis teridentifikasi sebagai bakteri Halomonas salina dan Vibrio alginolyticus.Kata Kunci : Padina australis, karakterisasi, bakteri simbion

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Xie, Hao, Sabine Buschmann, Julian D. Langer, Bernd Ludwig und Hartmut Michel. „Characterization of two isoforms of cbb3 cytochrome oxidase from Pseudomonas stutzeri ZoBell“. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1817 (Oktober 2012): S114. http://dx.doi.org/10.1016/j.bbabio.2012.06.306.

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Cai, M., und R. Timkovich. „Solution conformation of cytochrome c-551 from Pseudomonas stutzeri ZoBell determined by NMR“. Biophysical Journal 67, Nr. 3 (September 1994): 1207–15. http://dx.doi.org/10.1016/s0006-3495(94)80590-9.

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Stewart, Gregory J., und Christopher D. Sinigalliano. „Detection and characterization of natural transformation in the marine bacterium Pseudomonas stutzeri strain ZoBell“. Archives of Microbiology 152, Nr. 6 (November 1989): 520–26. http://dx.doi.org/10.1007/bf00425480.

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Kohlstaedt, Martin, Hao Xie, Sabine Buschmann, Anja Resemann, Julian D. Langer und Hartmut Michel. „Characterization of the two cbb3-type cytochrome c oxidase isoforms from Pseudomonas stutzeri ZoBell“. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1837 (Juli 2014): e99. http://dx.doi.org/10.1016/j.bbabio.2014.05.174.

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Tasia, Winda, Rina Zuraida und Yopi Yopi. „Isolasi Bakteri Pendegradasi Xilan dan Manan dari Perairan Indonesia“. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan 11, Nr. 1 (08.06.2016): 101. http://dx.doi.org/10.15578/jpbkp.v11i1.283.

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Bakteri laut penghasil enzim xilanase dan mananase menyimpan banyak potensi bagi bioteknologi kelautan. Informasi sebarannya juga dapat digunakan untuk memetakan keragaman bakteri laut serta analisis lingkungan perairan Indonesia sehingga pemanfaatannya dapat tepat sasaran. Penelitian ini bertujuan untuk mengisolasi bakteri laut pendegradasi xilan dan manan dari Indonesia. Penapisan kemampuan xilanolitik dan manolitik menggunakan metode Congo red dilakukan pada isolat bakteri yang diisolasi dari Laut Jawa, Selat Makassar, Laut Flores, dan Laut Sawu pada kedalaman 5 dan 20 m. Hasil penelitian menunjukkan bahwa isolat X7517, X1654, dan XM26511 diketahui menghasilkan xilanase dengan kondisi optimal reaksi enzim masing-masing adalah pH 9, T 90 °C (2,253±2,075 U/ml), pH 6, T 70 °C (0,633±0,082 U/ml), dan pH 6, T 70 °C (2,293±0,066 U/ml). Ketiganya diketahui memiliki kesamaan genetis dengan Halomonas aquamarina DSM 30161, Alteromonas macleodii NBRC 102226, dan H. meridiana NBRC 15608. Isolat bakteri manolitik L15203 dan L16571 memiliki kesamaan dengan Idiomarina zobellii KMM231, sedangkan isolat L2207 memiliki kesamaan dengan Bacillus sp. MB 71. Ketiga bakteri tersebut memiliki aktivitas mananase optimal pada kondisi alkali, masing-masing pada pH 8, T 80 °C (0,477±0,024 U/ml) untuk L 15203, pH 9,T 90 °C (0,476±0,009U/ml) untuk L16571 dan pH 9, T 80 °C (0,528±0,057 U/ml) untuk L2207. Bakteri laut xilanolitik dan manolitik yang berpotensi dalam produksi xilanase dan mananase melimpah di sebagian perairan Indonesia, terutama di perairan yang semakin dekat permukaan laut atau perairan dangkal.

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Miller, Gregory T., Donald Q. Mackay, Melissa S. Standley, Sherry L. Fields, Wendi M. Clary und Russell Timkovich. „Expression of Pseudomonas stutzeri Zobell cytochrome c-551 and its H47A variant in Escherichia coli“. Protein Expression and Purification 29, Nr. 2 (Juni 2003): 244–51. http://dx.doi.org/10.1016/s1046-5928(03)00065-2.

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José León, María, Fernando Martínez-Checa, Antonio Ventosa und Cristina Sánchez-Porro. „Idiomarina aquatica sp. nov., a moderately halophilic bacterium isolated from salterns“. International Journal of Systematic and Evolutionary Microbiology 65, Pt_12 (01.12.2015): 4595–600. http://dx.doi.org/10.1099/ijsem.0.000619.

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Four bacterial strains, SN-14T, SN-4, M6-46 and M6-58B, were isolated from water of ponds of two salterns located in Huelva (Spain). They were Gram-stain-negative, aerobic and slightly curved rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains belong to the genus Idiomarina, being related most closely to Idiomarina fontislapidosi F23T (98.4–98.0% sequence similarity), Idiomarina seosinensis CL-SP19T (98.3–98.0%), Idiomarina piscisalsi TPS4-2T (97.9–97.4%), Idiomarina baltica OS145T (97.5–97.4%) and Idiomarina zobellii KMM 231T (97.6–97.0%). The level of similarity with the type species of the genus, Idiomarina abyssalis KMM 227T, was 97.2–96.7%. The novel strains exhibited optimal growth at 5–10% (w/v) total salts, pH 7 and 37 °C. The major fatty acids of strain SN-14T were iso-C15 : 0, iso-C17 : 0, C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and iso-C17 : 1ω9c/C16 : 0 10-methyl. The DNA G+C content range was 47.6–50.8 mol%. The level of DNA–DNA relatedness between strain SN-14T and I. fontislapidosi F23T was 13%, while those between strain SN-14T and the other three new isolates were between 77 and 99%. These data demonstrated that the four isolates constitute a novel species of the genus Idiomarina. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the four strains represent a novel species of the genus Idiomarina, for which the name Idiomarina aquatica sp. nov. is proposed. The type strain is SN-14T ( = CCM 8471T = CECT 8360T = LMG 27613T).

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Hasmawaty, Hasmawaty. „Bioremediation of Liquid Waste Oil Through Bioreactor: A Case Study“. Current World Environment 11, Nr. 3 (25.12.2016): 715–19. http://dx.doi.org/10.12944/cwe.11.3.04.

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This article aims to explain the process of bioremediation of waste oils and found the rate of reaction kinetics model microbes. Samples were taken from liquid waste petroleum oil and gas companies Prabumulih South Sumatra, Indonesia. The isolation process using a medium Zobell’s and produces ten types of bacteria from the group Citrobacter sp, Micrococcaceaekarbon, and Flavobacterium sp. These bacteria’s is used in wastewater treatment bioreactor which have characteristic as follow: operate semi-continuously, using aeration column that contain polyurethane foam, variation of air discharge is 2 and 6 ml/sec, volume waste variation is 5 and 10 liters, 10 liters’ isolate, KNO3 (640 gram) and K2HPO4 (10 gram) as nutrition. The results showed that when bioremediation process contains 10 liters’ liquid waste, 10 liters’ isolate, and 6 ml/sec air discharge, it can reduce pH, specific gravity, BOD level, and the final weight of waste. Remediation process also increases the number of microbes, fatty oils and DO. Microbial maximum productivity is 59.50 with saturated concentration (Ks) at 3.25, and its slope is 0.43. Kinetics equation model following could be formulated as μ = 0.43/s/3.25+s.

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Devivilla, Suma, Jerusha Stephen, Manjusha Lekshmi, Sanath H. Kumar und Binaya Bhusan Nayak. „Evaluation of modified Zobell marine agar for differential isolation of histamine-forming bacteria from fresh fish“. Journal of Microbiological Methods 163 (August 2019): 105649. http://dx.doi.org/10.1016/j.mimet.2019.105649.

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Kim, Min Ju, Ha Ju Park, Pilsung Kang, Il Chan Kim, Joung Han Yim und Se Jong Han. „Purification and characterization of a new cold-active cellulolytic enzyme produced by Pseudoalteromonas sp. ArcC09 from the Arctic Beaufort Sea“. BioResources 17, Nr. 2 (20.04.2022): 3163–77. http://dx.doi.org/10.15376/biores.17.2.3163-3177.

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A cold-active endoglucanase-producing bacterium was isolated from the Beaufort Sea of the Arctic Ocean and identified as Pseudoalteromonas sp. ArcC09. Cellulolytic activity of ArcC09 reached a maximum of 60 U/mg when cultivated in ZoBell medium for 72 h at 15 °C. This purified endoglucanase, with a molecular mass of 28 kDa, exhibited maximum activity at pH 7.0 and 55 °C. The ArcC09 endoglucanase exhibited 10% and 36% of its maximal activity even at low temperatures of 5 °C and 15 °C, respectively. However, it showed lower thermal stability than a mesophilic cellulase, which is characteristic of a psychrophilic enzyme. The activity was inhibited by CuSO4, and linear alkylbenzene sulfonate (LAS). These findings supplement the understanding of cold-active endoglucanases and may have commercial applications in enzymatic digestion of cellulosic biomass to fermentable sugars.

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