Dissertationen zum Thema „Wooden track“

Railway sleepers have important roles within the complex railroad. Dynamic interaction among Rail, sleeper and ballast supports are vital for the construction of dynamic model track capable of predicting its responses to impact loads due to wheel flats, wheel burns, irregularities of the rail. Railway track deteriorates over time due to the dynamic loading of passing wheeled vehicle. to ensure the secure passing of trains, the track must well design and managed.The objective of this thesis is to determine the stress in wooden rail sleeper by the considering Ballast, Sub ballast, and soil with the help of well-known FEM Tool Ansys Workbench. Material models for wooden sleeper and subgrade are anisotropic elasticity and elasto plastic (Drucker Prager), respectively. To validate FEM results with analytical Zimmermann method, perform the 2D linear static analysis and 3D nonlinear static analysis. The numerical analysis results in Ansys Work bench are presented and discussed the model validation with analytical results. Even though simulation converged it does not mean this model could give results similar to reality. But in my opinion these results are not sufficient to study the behavior of wooden sleeper because limitations in soil mechanics (Soil Models in Ansys Workbench).

The topic of this diploma thesis is the architectural study of the cycling arena – velodrome – as a part of The Hněvkovský sports ground in Brno-Komárov. The new velodrome is situated next to the existing bicycle motocross ground and serves as a facility for the track cycling, indoor cycling and BMX. The core of the velodrome consists of the 250-meter wooden steeply banked track, which, together with other sports ground’s parameters fits the requirements of International Cyling Uninon for hosting international competitions. Cycling arena includes premises for athletes, spectators (stands are designed for 2700 spectators) and Favorit Brno administration and also bicycle storage and service areas. Facility includes also gym, fitness room, wellness and physiotherapy, athletes‘ accomodation. The velodrome is roofed with the arch-formed membrane anchored to a robust bond beam, that is brought to the ground in the front part of the building. This V-shaped bond beam together with the arched membrane geometry defines the building dynamic expression.

Thesis (Ph. D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Biology; and Woods Hole Oceanographic Institution), 2000.
Includes bibliographical references.
The marine cyanobacteria Synechococcus and Prochlorococcus are important primary producers in oligotrophic oceans. The abundance and cell division rates of these cyanobacteria can be influenced by trace metals such as iron and copper. Iron is an essential trace metal that is present in the high nutrient, low chlorophyll waters of the equatorial Pacific in extremely low concentrations. When these waters were enriched with iron, Prochlorococcus chlorophyll fluorescence per cell and cell size increased. Cell division rates doubled inside the iron enriched patch and reached two divisions per day in bottle incubations with additional iron, indicating that Prochlorococcus were iron limited. However, cell numbers remained constant because mortality rates nearly doubled after the addition of iron and essentially matched the increases in cell division rate. Trace metals can also be present in toxic, rather than limiting concentrations. Copper is an essential trace element that is toxic to cyanobacteria in pM quantities. In stratified water columns in the Sargasso Sea, free Cu2+ concentrations are high in the mixed layer (up to 6pM) and most of the Prochlorococcus population is located below the thermocline where free Cu2+ concentrations are lower. The distribution of Synechococcus is more uniform with depth. Prochlorococcus isolates were more sensitive to copper than Synechococcus, but members of the low chi BIA (high light adapted) ecotype were less sensitive than strains with high chi BIA ratios (low light adapted). In the field, the in situ concentration of free Cu2+ had a strong effect on the copper sensitivity of Prochlorococcus. Net growth rates were substantially reduced when Prochlorococcus from environments where the in situ free Cu2+ was low (deep mixed layers and below the thermocline in stratified water) were exposed to copper. Prochlorococcus in shallow mixed layers where in situ Cu2 + was high were less sensitive to copper and may have been members of the copper resistant low chi B/ A ecotype. Synechococcus were relatively copper resistant across a range of environments. These data are consistent with the hypothesis that ambient copper levels may influence the relative abundance of Prochlorococcus and Synechococcus in the Sargasso Sea.
by Elizabeth Lowell Mann.
Ph.D.

Thesis: Ph. D., Joint Program in Chemical Oceanography (Massachusetts Institute of Technology, Department of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2014.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Marine particles include all living and non-living solid components of seawater, representing an extremely dynamic and chemically diverse mixture of phases. The distributions of these phases are poorly constrained and undersampled in the oceans, despite interactions between living organisms and non-living minerals having central roles within many globally relevant biogeochemical processes. Through a combination of method development, basin-scale particulate collection and analyses, modeling, and field experiments, this thesis examines both the distributions of marine particulate trace metals and the underlying processes-inputs, scavenging, vertical and horizontal transport, and biotic uptake-in which marine particles participate. I first present the results of an intercalibration exercise among several US laboratories that analyzed filtered particles on shared polyethersulfone filters. We use inter-lab and intra-lab total elemental recoveries of these particles to determine our state of our intercalibration (by Daniel Chester Ohnemus.
Ph. D.

The extraction and recovery of trace organic material from semi-solid and solid matrices is often the slowest and most error-prone step of an analytical method. The conventional liquid extraction techniques for solids and semi-solids materials (Soxhlet) have two main disadvantages. The first, large volumes of organic solvent are required, which can lead to sample contamination and "losses" due to volatilization during concentration steps. The second, to achieve an exhaustive extraction can require several hours to days. With the development of sophisticated instrumentation with detection limits in the picogram and femtogram levels, pressure is finally felt within the analytical community to develop and validate sample preparation procedures which can be used to rapidly isolate trace level organics from complex matrices.Because of its applicability to solid, semi-solid, and liquid matrices microwave-assisted (MAE) extraction has emerged as a powerful sample preparation technique. The objective of this research was to evaluate directly focused microwave energy for the isolation of systemic fungicide residues from woody plant tissues.The hallmark of microwave extraction (MAE) is accelerated dissolution kinetics as a consequence of the rapid heating processes that occur when a microwave field is applied to a sample. The current popularity of MAE resides mainly on its applicability to a wide range of sample types because the selectivity can be easily manipulated by altering solvent polarities.Propiconazole is a systemic fungicide, used to combat the fungal pathogen Ophiostoma ulmi, the casual agent of Dutch elm disease (DED). It was successfully extracted from treated Ulmus americana (elm tree) using MAE with a percent recovery of 395% in 15 minutes. Until now, techniques for rapid and efficient extraction of polar material from wood were non-existent. This work produces results much quicker than Supercritical Fluid Extraction (SFE). The influence of pH, microwave power, and time on extraction efficiency was also investigated. The extraction methodology was optimized and statistically validated.This MAE method combined with GC-MS was used to study the diffusion patterns and degradation of propiconazole in tree bark over extended time periods. Because of the complex nature of woody plant systems, it was realized that a more theoretical means must be used to determine the degradation rate of propiconazole in water systems. As a result, propiconazole was reacted with water under controlled temperature and pH conditions; to measure the degradation rate of propiconazole.The internal pH of elm sap is about 6.0; the slightly acidic environment and natural enzymes within the xylem vessels are known to catalyze the degradation of propiconazole (1). Novartis Inc. has marketed propiconazole as having fungicidal effects in injected elms for nearly two years. Our degradation studies have indicated much shorter lifetimes. To confirm our fate studies, the activation energy for the degradation reaction of propiconazole was calculated. This information provided valuable insight into revising dosage and treatment frequency for maximum protection of the elm against Dutch elm disease. Anti-fungal activity among metabolites was also explored. This is the first reported use of MAE to monitor the degradation of systemic pesticides in woody plant material.
Ph. D.

Optimization and improvement techniques have been employed to improve truck transportation in the forest industry. Most studies in this area focus on log transportation and there is not much literature available on wood chip transportation which is distinct from log transportation in several ways : chip-truck transportation usually considers a longer planning period, requires a more complex driver scheduling, involves strict limitations on truck unloading capacity, and considers service priorities for major suppliers. The objective of this thesis is to model and improve truck transportation of wood chips to a pulp mill. To achieve this, a network that transports wood chips from a number of sawmills to a pulp mill with limited truck unloading capacity was studied and an optimization model was developed to consider wood chip production plan at supplier sawmills, transportation of wood chips and unloading at the pulp mill. A simulated annealing metaheuristic was adapted to solve the model and the results for a case study in British Columbia are presented. The results of the metaheuristic showed that the total penalties for truck waiting times could be reduced by 7 percent ($31,000) compared to solutions obtained through simulation. The results also suggested that the fleet size could be reduced by one-third of the actual fleet size used by the pulp mill. In order to explore the impact of various structural parameters on the transportation network, sensitivity and scenario analyses were used to study the impacts of fleet size, an additional truck dumper, truck types, and switch point locations for truck drivers. The results indicated that the pulp mill could reduce its fleet size and an additional truck dumper could reduce transportation costs. The study also suggested that half of the fleet should be replaced by self-unloading trucks and the investigation of the switch point locations for drivers indicated that the existing switch points were the best available options. The simulated annealing model could assist the pulp mill truck dispatchers to achieve better transportation plans. The scenario and sensitivity analysis could help the pulp mill manager to adapt the most profitable changes in the structure of the transportation network.
Forestry, Faculty of
Graduate

Thesis: Ph. D. in Marine Geology, Joint Program in Marine Geology and Geophysics (Massachusetts Institute of Technology, Department of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2017.
Cataloged from PDF version of thesis.
Includes bibliographical references.
This thesis analyzes compositions of seafloor massive sulfide (SMS) deposits and related hydrothermal vent fluids to identify proxies of reaction zone conditions (host-rock lithology, hydrothermal fluid temperature and chemistry). Chapter 2 investigates the morphology, mineralogy, and geochemistry of SMS deposits from six vent fields along the Eastern Lau Spreading Center (ELSC), demonstrating that ELSC SMS deposits record differences in hydrothermal fluid temperature, pH, sulfur fugacity and host-rock lithology related to proximity to the nearby Tonga Subduction Zone. Chapters 3 and 4 focus on partitioning of Co, Ni, Ga, Ag, and In between hydrothermal vent fluids and chalcopyrite lining fluid conduits in black smoker chimneys. Chapter 3 develops secondary ion mass spectrometry (SIMS) as a technique to measure Co, Ni, Ga, Ag, and In in chalcopyrite and identifies a correlation between Ga and In in chalcopyrite and hydrothermal fluid pH. Chapter 4 presents new data on these elements in ELSC hydrothermal fluids that, combined with SIMS analyses of chalcopyrite chimney linings and previously published data on vent fluids from the Manus Basin, provide evidence that supports partitioning of Ag a lattice substitution for Cu. Together, concentrations of Ga, In, and Ag in chalcopyrite provide proxies of hydrothermal fluid pH and metal (i.e., Ag and Cu) contents.
by Guy Nathaniel Evans.
Ph. D. in Marine Geology

This thesis project aims to repurpose an abandoned railway tunnel in Eriksdalslunden, Stockholm into an experimental activity/farming center for the purpose of teaching people of all ages different farming methods. The learning center implements exciting farming techniques such as aquaponics, where plants get their nutrients from live fish.  This center also teaches about the new technologies that make it possible to grow plants beneath the earth´s surface. These technologies harness the benefits of the sun into a concentrated form and then leads it deep underground. Repurposing unused underground spaces such as this can help develop our cities in a more efficient way.
Detta avhandlingsprojekt syftar till att omarbeta enövergiven järnvägstunnel i Eriksdalslunden,Stockholm till en experimentell verksamhet / jordbrukscentrum för att undervisa människor i alla åldrar olika odlingsmetoder. Inlärningscentralen implementerar spännande odlingstekniker som aquaponics, där växter får sinanäringsämnen från levande fisk.Detta centrum lär också om den nya tekniken som gör det möjligt att växa växter under jordens yta. Dessa tekniker utnyttjar solens fördelar i en koncentrerad form och leder sedan den djupt under jorden. Repurposing oanvända underjordiska utrymmen som detta kan hjälpa till att utveckla våra städer på ett mer effektivt sätt.

Thesis: Ph. D., Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Department of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2018.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Nutrients and trace metals are vital for supporting life in the ocean, but the boundary processes that control the distributions of these elements are poorly constrained. Radium isotopes are well suited to studying inputs of elements from ocean margins because they are produced in sediments and soluble in seawater. The half-lives of the four isotopes (223Ra, 224Ra, 228Ra, 226Ra) range from days to thousands of years, thus these isotopes can be used to study oceanographic processes occurring over a range of time scales. In this thesis I have applied the quartet of radium isotopes to investigate boundary inputs, including seafloor hydrothermal vents, continental shelves, and rivers. First, radium isotope ratios were used to constrain the age of hydrothermal plumes emanating from vents along the Mid-Atlantic Ridge and East Pacific Rise. These radium-derived ages were applied to determine the iron residence time in the Pacific plume that emanates from near 15°S, providing an important constraint on the hydrothermal delivery of iron to the deep ocean. Next, 228Ra was used to show that shelf inputs to the Arctic Ocean have increased over the last decade, implying that the fluxes of other shelf-derived materials are also increasing and could impact primary production in this basin. The ratio of 228Ra and 226Ra was also applied to determine the ventilation time of Arctic intermediate waters with respect to shelf inputs, and the first measurements of 226Ra in the deep Canada Basin were used to estimate the residence time of deep waters with respect to benthic sediment inputs. Finally, a study of the Mackenzie River Estuary illustrated the importance of deltaic and estuarine processing on the ultimate delivery of nutrients, trace metals, dissolved organic matter, and radium to the Arctic Ocean. By applying radium isotopes as tracers of boundary inputs in these diverse environments, the work presented in this thesis improves our understanding of nutrient and trace metal inputs to the coastal and open ocean.
by Lauren Elizabeth Kipp.
Ph. D.

Incineration is a mainstream strategy for solid waste management in Sweden and all over the world. Improved knowledge and understanding about the distribution of trace elements (in ashes) during incineration, and how trace element partitioning respond to the changes in waste composition, are important in terms of combustion process optimization and plant efficiency. Moreover, determination of chemical association of trace elements in ashes are vital for avoiding environmental concerns and to promote possible reuse. In this study, partitioning of trace elements in ashes during incineration as function of input waste fuel and incineration technology was investigated. Further, chemical association of trace elements in resulting ashes was studied. An evaluation was also performed about feasibility of metal extraction from sulfidic mining waste and flotation tailings. Moreover, green liquor dreg (GLD) was tested with respect to stabilization of metals within the sulfidic mining waste. Findings showed that the total input of trace elements and chlorine affects the partitioning and increasing chlorine in the input waste caused increase in transfer of trace elements to fly ash especially for lead and zinc. Vaporization, condensation on fly ash particles and adsorption mechanisms play an important role for metal distribution. Firing mixed waste, especially biofuel mix, in grate or fluidized (CFB) boilers caused increased transfer into fly ash for almost all trace elements particularly lead and zinc. Possible reasons might be either an increased input concentration of respective element in the waste fuel, or a change in volatilization behavior due to the addition of certain waste fractions. Chemical association study for fly ashes indicated that overall, Cd, Pb, Zn, Cu and Sb are presenting major risk in most of the fly ashes, while in bottom ashes, most of elements are associated with stable fraction. Further, fuel type affects the association of elements in ashes. Chemical leaching of mining waste materials showed that sulfuric acid (under different conditions) is the best reagent to recover zinc and copper from sulfidic mining waste and also copper from flotation tailings. GLD indicates potential for metal stabilization in mining waste by reducing the metal mobility. Extraction methods could be applied to treat mining waste in order to meet the regulatory level at a specific mining site.Similarly stabilization/solidification  methods might be applied after leaching for recovery of metals.

This report describes an annual line detection algorithm for the WoodEye quality control system. The goal with the algorithm is to find the positions of annual lines on the four surfaces of a board. The purpose is to use this result to find the inner annual ring structure of the board. The work was done using image processing techniques to analyze images collected with WoodEye. The report gives the reader an insight in the requirements of quality control systems in the woodworking industry and the benefits of automated quality control versus manual inspection. The appearance and formation of annual lines are explained on a detailed level to provide insight on how the problem should be approached. A comparison between annual rings and fingerprints are made to see if ideas from this area of pattern recognition can be adapted to annual line detection. This comparison together with a study of existing methods led to the implementation of a fingerprint enhancement method. This method became a central part of the annual line detection algorithm. The annual line detection algorithm consists of two main steps; enhancing the edges of the annual rings, and tracking along the edges to form lines. Different solutions for components of the algorithm were tested to compare performance. The final algorithm was tested with different input images to find if the annual line detection algorithm works best with images from a grayscale or an RGB camera.

The scope of these studies was two-fold: to evaluate high pressure homogenized slurries as a rapid means of screening wood pulps for their Cu, Mn and Fe content and to evaluate the high pressure homogenization in combination with chelating agents or enzymatic digestion for the liberation/extraction of metal ions from this matrix.
Cu, Mn and Fe were determined successfully in pulp samples using high-pressure homogenization prior to slurry introduction-GF-AAS. The analysis time of the method from sample acquisition to determination was of the order of a few minutes per sample.
Different cellulose swelling/dissolution agents were evaluated for the generation of quasi-stable pulp suspensions, rich in cellulose. High-pressure homogenization alone or in combination with chelating agents or enzymatic digestion was also investigated as a means of quantitatively releasing these metal ions into the liquid phases.
A new model of homogenizer equipped with ceramic homogenizing valve with a few modifications was evaluated in terms of metal contamination levels within the final sample dispersion. (Abstract shortened by UMI.)

The submission of this thesis was preceded by a specialised studio „Brněnské nábřeží a kulturní čtvrť Ponava“. This work focused on a complex urban architectural solution to the area around the former “Jaselská kasárna”, the urban context, transport services, the field configuration etc. The thesis follows this project in a section defined by streets U Červeného mlýna, Staňkova and Střední. The goal of the thesis lies in the creation of an intensive goal – a cultural area, which will offer a high-quality space for intergenerational communication and new social experiences and activities. It newly connects the urban part Ponava with the town center of Brno by a high-quality axis for pedestrian and cycling transport. The project of this cultural area arose after a thorough analysis of surrounding, intercity and global relations as a combination of aims: for children – the elementary art school with graphic and dancing fields, for seniors – leisure time center, and for all together - a multifunctional concert hall and a revitalized area of the former industrial zone of furniture production to a multipurpose intergenerational area with a modern art gallery, offices for young businessmen, architects and artists, and a music club in the basement of the gallery.

Belastningen vid tryck vinkelrätt mot fiberriktningen är ett problem vid höga träbyggnader med träregelstomme vid till exempel mötet mellan regel och syll. Ettmaterial som blivit alltmer populärt i Sverige är korslimmat trä, som benämns som KL-trä, där lameller korsvis limmas samman till en skiva. Experiment har tidigare utförts på materialet och det har visat att en bräda av KL-trä, som idag inte tillverkas, klarar ett högre stämpeltryck än en vanlig bräda av konstruktionsvirke, vilket skulle kunna vara en lösning på problemen vid tryck vinkelrätt fiberriktningen i höga träbyggnader. Syftet med detta examensarbete är att bestämma KL-träets karakteristiska tryckkraftskapacitet och tryckhållfasthet för två uppställningar av KL-trä där andelar vinkelräta och parallella lameller som belastas i tvärsnittet skiljer sig åt. Det undersöks också hur lasten fördelar sig på lamellerna som belastas parallellt med fiberriktningen och vinkelrätt mot fiberriktningen i en bräda av KL-trä samt att teoretiska beräkningar av tryckkraftskapaciteten utfördes i mitten och i änden på brädan. Examensarbetet omfattades av tre olika metoder där standarden SS-EN 408 för tryck vinkelrätt mot fiberriktningen användes för att bestämma tryckkraftskapacitet och tryckhållfasthet för de två uppställningarna av KL-trä genom materialtester i en hydraulisk press. Lastfördelningen mellan lamellerna i en KL-trä bräda undersöktes genom stämpeltrycktester i änden på brädan och återspeglar ett verkligt möte mellan regel och syll. En solid bräda av KL-trä, en bräda där de vinkelräta lamellerna sågats bort samt en bräda av konstruktionsvirke belastades för att undersöka differensen i stämpeltryck. Teoretiska beräkningar utfördes på KL-trä brädorna både i mitten och i änden genom att de olika lamellerna beräknades som enskilda brädor enligt Eurokod 5 samt EKS11 och därefter summerades alla lameller för KL-trä brädan. Resultaten visade att den uppställningen av KL-trä med störst andel lameller som belastades parallellt med fiberriktningen erhöll störst tryckkraftskapacitet och fick högst tryckhållfasthet. I brädan av KL-trä tog de lameller som belastades parallellt med fiberriktningen upp ungefär fyra femtedelar av lasten i tvärsnittet som belastades. De teoretiska beräkningarna som utfördes gav en lägre tryckkraftskapacitet än resultaten från de experimentella testerna. För materialtest 1 där lamellerna fördelade sig 58 % vinkelräta lameller och 42 % parallella lameller erhölls karakteristiska värden på 79,7 kN på tryckkraftskapacitet och 18,6 MPa på tryckhållfasthet. För materialtest 2 där lamellerna fördelade sig 42 % vinkelräta lameller och 58 % parallella lameller erhölls karakteristiska värden på 114,6 kN på tryckkraftskapacitet och 26,8 MPa på tryckhållfasthet. Belastningsfördelningen i KL-trä brädan visade att de parallellt belastade lamellerna tog upp en last motsvarande 80,8 % och de vinkelräta lamellerna tog upp en last motsvarande 19,2 %. De teoretiska beräkningarna utnyttjades till 57 % i jämförelse mot maxlasten från de experimentella testerna, både i mitten och på brädans ände.
Compression perpendicular to the grain is today a problem with tall wood buildings with timber-frame at, for example, the meeting between timber stud and the bottom rail. A material that has become increasingly popular in Sweden is cross-laminated timber, which is referred to as CLT, where lamellae are glued together crosswise to a board. Experiments have previously been performed on the material and it has shownthat a board made of CLT, which is not manufactured today, can withstand a higher pressure than an ordinary board made of structural timber, which could be a solution to the problems with compression perpendicular to the grain in tall wood buildings. The purpose of this thesis is to determine the CLTs characteristic compressive force capacity and compressive strength for two formations of CLT where proportions of perpendicular and parallel lamellae that are loaded in the cross section differ. It will also be investigated how loads are distributed on the lamellae which are loaded parallel to the grain and perpendicular to the grain in a board made of CLT and that theoretical calculations of the compressive force capacity were performed in the middle and at the end of the board. The thesis was comprised of three different methods where the standard SS-EN 408 for compression perpendicular to the grain was used to determine compressive forcecapacity and compressive strength for the two formations of CLT through material tests in a hydraulic press. The load distribution between the lamellae in a CLT-boardwas examined by compression tests at the end of the board and reflects a real meeting between a timber stud and the bottom rail. A solid board made of CLT, a board where the perpendicular lamellae were sawn off and a board made of structural timber were loaded to investigate the difference in compression. Theoretical calculations were performed on the CLT-boards both in the middle and at the end through calculating the different lamellae as individual boards according to Eurocode 5 and EKS11 and then all lamellae for the CLT-board were summed. The results showed that the arrangement of CLT with the largest proportion of lamellae parallel to the grain obtained the largest compressive force capacity and had the highest compressive strength. In the CLT-board, the parallel-loaded lamellae took up about four-fifths of the load in the cross-section that was loaded. The theoretical calculations performed gave a lower compressive force capacity than the results of the experimental tests. For material test 1 where the lamellae were distributed 58% perpendicular lamellaeand 42% parallel lamellae, characteristic values of 79.7 kN on compressive capacity and 18.6 MPa on compressive strength were obtained. For material test 2 where the lamellae were distributed 42% perpendicular lamellae and 58% parallel lamellae, characteristic values of 114.6 kN on compressive capacity and 26.8 MPa on compressive strength were obtained. The load distribution in the CLT-plank shown that the parallel-loaded lamellae took up a load corresponding to 80.8% and the perpendicular lamellae took up a load corresponding to 19.2%. The theoretical calculations were used to 57% in comparison with the maximum load from the experimental test, both in the middle and at the end of the board.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O grande desafio do sistema de defesa respiratório é a manutenção dos animais em baias. Pois, devido a diversos fatores, acaba por aumentar as chances do desenvolvimento de afecções respiratórias e a qualidade do material utilizado como cama é fator agravante. Diante disto, três grupos com 5 equinos cada foram submetidos a 45 dias sob o mesmo manejo, sendo dois grupos estabulados com diferentes tipos de cama, um com maravalha esterilizada (ME) e o outro com maravalha não esterilizada (MNE), e o terceiro grupo a pasto (Pasto). Foram realizadas análises do lavado broncoalveolar (LBA) (celularidade e marcadores de estresse oxidativo) e hemograma antes do início do manejo (Basal) e posteriormente a cada 15 dias (M15, M30 e M45). Também foi avaliada a presença de gêneros fúngicos nas amostras de cama, e do feno utilizado na alimentação dos animais. Os hemogramas permaneceram dentro dos valores da normalidade. Os valores obtidos de malondialdeído (MDA) e ácido úrico no LBA não apresentaram diferença entre os momentos e grupos avaliados. A vitamina C no LBA apresentou queda em seus valores em M30. Os três grupos apresentaram queda nas concentrações de glutationa reduzida em M30, tendo havido diferença significativas entre os grupos Pasto e MNE. Os grupos ME e MNE apresentaram queda nos valores de glutationa oxidada em M45, já o grupo Pasto apresentou queda constante a partir de M15, com diferença significativa em relação ao grupo ME em M30. A superóxido dismutase apresentou aumento em M30 no grupo MNE, levando a diferença significativa em relação aos grupos Pasto e ME. A glutationa peroxidase no LBA apresentou queda significativa no grupo ME em M45. Quanto a celularidade do LBA a contagem de células nucleadas totais e eosinófilos não apresentaram diferenças significativas. O grupo ME apresentou elevação nos valores de neutrófilos em M30, levando a diferença significativa em relação aos grupos MNE e Pasto, já o grupo MNE apresentou queda significativa em M45. Os grupos ME e MNE apresentaram queda nos valores de linfócitos no LBA em M30, já o grupo Pasto apresentou queda em M15. O grupo Pasto apresentou aumento dos macrófagos no LBA em M15 e M30. As análises das amostras de cama e feno apresentaram baixas porcentagens de gêneros fúngicos. Grande parte das alterações apresentadas ocorreram em M30, momento em que foram registradas as menores temperaturas e maiores velocidades dos ventos durante todo período experimental, o que possivelmente levou a um desequilíbrio oxidativo pontual, com pequenas variações na celularidade do LBA. Acredita-se que o manejo, as boas condições de higiene e ventilação das baias tenham contribuído para que não houvesse o desenvolvimento de alterações inflamatórias no sistema respiratório dos animais avaliados. Deste modo, podemos concluir que não houve diferenças significativas na manutenção dos animais nos diferentes tipos de cama em relação a resposta inflamatória, estresse oxidativo e desenvolvimento fúngico.
The great challenge of the respiratory defense system is the maintenance of animals in stalls, which due to several factors ends up increasing the chances of development of respiratory diseases. The quality of the material used as bed is an aggravating factor. Three groups of 5 horses were submitted to 45 days under the same management, and two groups were housed with different types of beds, one with sterilized wood shaving (ME) and the other with unsterilized wood shaving (MNE), and the third group was maintened in the pasture. Bronchoalveolar lavage fluid (BALF) and hemoglobin analyzes were performed before baseline and then every 15 days (M15, M30 and M45). It was also evaluated the possible presence of fungal genera in bed samples, and in hay used in animal feeding. The hemograms remained within normal values. The values of malondialdehyde (MDA) and uric acid in BALF showed no difference between the moments and groups evaluated. Vitamin C in BALF showed a decrease in M30 values. The three groups showed a decrease in the concentrations of glutathione reduced in M30, and there were significant differences between the groups pasture and MNE. The ME and MNE groups showed a decrease in the values of oxidized glutathione in M45, whereas the pasture group presented a constant drop from M15, with a significant difference in relation to the ME group in M30. Superoxide dismutase increased in M30 in the MNE group, leading to a significant difference in relation to the pasture and ME groups. Glutathione peroxidase in BALF showed a significant decrease in the ME group in M45. As for the cellularity of BALF, total nucleated and eosinophil counts did not showed significant differences. The ME group presented elevation in neutrophil values in M30, leading to a significant difference in relation to the MNE and pasture groups, whereas the MNE group presented a significant decrease in M45. The ME and MNE groups presented a decrease in lymphocyte values in the BALF in M30, whereas the pasture group presented a decrease in M15. The pasture group presented increase of the macrophages in the BALF in M15 and M30. Bed and hay samples showed low percentages of fungal genera. Most of the alterations presented occurred in M30, at which time the lowest temperatures were recorded throughout the experimental period, possibly leading to a punctual oxidative imbalance, with small variations in BALF cellularity. It is believed that management, good conditions of hygiene and ventilation of the boxes contributed to the no development of inflammatory changes in the respiratory system of the animals evaluated. Thus, we can conclude that there were no significant differences in the maintenance of the animals in the different types of bed in relation to the inflammatory response, oxidative stress and fungal development.

Thesis (Ph. D.)--Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Dept. of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2013.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Pb and Pb isotopes in the ocean have varied on decadal to centennial time scales due to anthropogenic Pb inputs. Thus, tracing the temporal variation of Pb and Pb isotopes in the ocean provides information on the major sources of Pb and the transport of Pb from sources to the ocean surface and into the ocean interior. In this thesis study, first, a method was developed for the analysis of dissolved Pb and other trace elements in seawater using single batch nitrilotriacetate resin extraction and isotope dilution ICP-MS, which was applied in analyzing seawater Pb concentrations in the rest of the study. A -550 year history of the Pb and Pb isotopes in the deep North Atlantic Ocean is reconstructed using a deep-sea coral, showing the infiltration of anthropogenic Pb to deep sea. Comparing the results to the surface North Atlantic Ocean Pb record using a Transit Time Distribution model, the mean transit time of Pb is estimated to be -64 years. This is longer than the transit time estimate assuming simple advection from a source, showing the importance of advective-diffusive mixing in the transport of Pb to the ocean interior. The later part of the thesis investigates Pb in the Indian Ocean, where no useful Pb data have been previously reported. First, using annually-banded surface growing corals, I reconstruct variations of Pb and isotopes in the surface waters of the central and eastern Indian Oceans during the past half-century. Results of the study show the increase of Pb concentrations from the mid-1970s, and major sources of the Pb are discussed, including leaded gasoline and coal burning, based on their emission histories and Pb isotope signatures. Second, Pb concentration and isotope profiles are presented from the northern and western Indian Oceans. Higher Pb concentrations and lower Pb isotope ratios (206Pb/ 207Pb, 208Pb/207Pb) are found in the upper water column (by Jong-Mi Lee.
Ph.D.