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Zeitschriftenartikel zum Thema „VvCRF4“

Autor: Grafiati

Veröffentlicht am 29. März 2025

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Shi, Wenjing, Michael Riemann, Sophie-Marie Rieger und Peter Nick. „Cold-Induced Nuclear Import of CBF4 Regulates Freezing Tolerance“. International Journal of Molecular Sciences 23, Nr. 19 (27.09.2022): 11417. http://dx.doi.org/10.3390/ijms231911417.

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C-repeat binding factors (CBFs) are crucial transcriptional activators in plant responses to low temperature. CBF4 differs in its slower, but more persistent regulation and its role in cold acclimation. Cold acclimation has accentuated relevance for tolerance to late spring frosts as they have become progressively more common, as a consequence of blurred seasonality in the context of global climate change. In the current study, we explore the functions of CBF4 from grapevine, VvCBF4. Overexpression of VvCBF4 fused to GFP in tobacco BY-2 cells confers cold tolerance. Furthermore, this protein shuttles from the cytoplasm to the nucleus in response to cold stress, associated with an accumulation of transcripts for other CBFs and the cold responsive gene, ERD10d. This response differs for chilling as compared to freezing and is regulated differently by upstream signalling involving oxidative burst, proteasome activity and jasmonate synthesis. The difference between chilling and freezing is also seen in the regulation of the CBF4 transcript in leaves from different grapevines differing in their cold tolerance. Therefore, we propose the quality of cold stress is transduced by different upstream signals regulating nuclear import and, thus, the transcriptional activation of grapevine CBF4.

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Aazami, Mohammad Ali, und Nasser Mahna. „Salicylic acid affects the expression of VvCBF4 gene in grapes subjected to low temperature“. Journal of Genetic Engineering and Biotechnology 15, Nr. 1 (Juni 2017): 257–61. http://dx.doi.org/10.1016/j.jgeb.2017.01.005.

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Hu, Xiaoxuan, Chenyu Hao, Zong-Ming Cheng und Yan Zhong. „Genome-Wide Identification, Characterization, and Expression Analysis of the Grapevine Superoxide Dismutase (SOD) Family“. International Journal of Genomics 2019 (24.02.2019): 1–13. http://dx.doi.org/10.1155/2019/7350414.

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Superoxide dismutase (SOD) is an essential enzyme of the plant antioxidant system that responds to oxidative damage caused by adverse conditions. However, little is known about the SOD gene family in Vitis vinifera (Vv). In the present study, ten SOD genes, including 6 copper/zinc SODs, 2 iron SODs, and 2 manganese SODs, were identified in the grapevine genome where they were unevenly distributed on 12 chromosomes. Ten VvSOD genes were divided into three main groups based on phylogenetic analysis, subcellular localization, and the distribution of conserved protein motifs. Additionally, many cis-elements related to different stresses were found in the promoters of the 10 VvSOD genes. Syntenic analysis revealed that VvMSD1 and VvMSD2 were derived from segmental duplication, and VvCSD4 and VvCSD5 belong to a pair of tandemly duplicated genes. Gene expression analysis based on microarray data showed that the 10 VvSOD genes were expressed in all the tested tissues. Interestingly, the segmentally duplicated gene pair (VvMSD1 and VvMSD2) exhibited differential expression patterns in various organs. In contrast, the tandemly duplicated gene pair (VvCSD4 and VvCSD5) displayed similar expression patterns in the tested organs. Our results provide a basis for further functional research on the SOD gene family in grapevine.

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Tillett, Richard L., Matthew D. Wheatley, Elizabeth A. R. Tattersall, Karen A. Schlauch, Grant R. Cramer und John C. Cushman. „The Vitis vinifera C-repeat binding protein 4 (VvCBF4) transcriptional factor enhances freezing tolerance in wine grape“. Plant Biotechnology Journal 10, Nr. 1 (13.09.2011): 105–24. http://dx.doi.org/10.1111/j.1467-7652.2011.00648.x.

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Li, Songqi, Xufei Li, Pengwei Jing, Min Li, Yadan Sun, Leilei Wang, Qiaofang Shi und Yihe Yu. „The Transcription Factor VvbHLH053 Regulates the Expression of Copper Homeostasis-Associated Genes VvCTr5/6 and VvFRO4 and Confers Root Development in Grapevine“. International Journal of Molecular Sciences 26, Nr. 1 (26.12.2024): 128. https://doi.org/10.3390/ijms26010128.

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Chlormequat chloride (CCC) has been demonstrated to inhibit plant growth and strengthen seedlings. The present study demonstrated that the root growth of Thompson seedless grapevine seedlings was significantly enhanced by the application of CCC treatment. Nevertheless, the precise mechanism by which CCC regulates plant root growth remains to be elucidated. Consequently, an RNA-sequencing (RNA-Seq) analysis was conducted on grapevine roots subjected to CCC treatment and those undergoing natural growth. A total of 819 differentially expressed genes were identified. Subsequently, Gene Ontology (GO) functional enrichment and weighted gene co-expression network analysis (WGCNA) identified the Copper (Cu) homeostasis-associated genes, VvCTr4/5/6/8 and VvFRO4, which play a pivotal role in mediating the effect of CCC. To further elucidate the transcription factor regulating these Cu homeostasis-associated genes, the key transcription factor VvbHLH053 was identified based on the PlantTFDB database, WGCNA results, and expression patterns under CCC treatment. Furthermore, multiple bHLH binding sites were identified on the promoters of VvCTr4/5/6 and VvFRO4. The GUS activity analysis and dual-luciferase assay demonstrated that VvbHLH053 can directly regulate the expression of VvCTr5/6 and VvFRO4. These findings reveal the feedback mechanism of grapevine root growth mediated by CCC and establish a direct functional relationship between CCC, VvbHLH053, and Cu homeostasis-associated genes that regulate root growth.

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Neba, Yasuhiko, Hirokazu Matsumoto, Ryozo Itoh und Kouichi Ishizaka. „VVCF AC-AC Converters Using Single Bridge Circuit“. IEEJ Transactions on Industry Applications 130, Nr. 6 (2010): 824–25. http://dx.doi.org/10.1541/ieejias.130.824.

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Neba, Yasuhiko, Hirokazu Matsumoto, Ryozo Itoh, Kouichi Ishizaka, Koichiro Hashimoto und Daiki Kaji. „VVCF Single-phase Current Source Converter using Single Bridge“. IEEJ Transactions on Industry Applications 132, Nr. 3 (2012): 452–53. http://dx.doi.org/10.1541/ieejias.132.452.

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Fu, Peining, Wenjie Wang, Lixia Hou und Xin Liu. „Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.“ Acta Societatis Botanicorum Poloniae 82, Nr. 4 (2013): 295–302. http://dx.doi.org/10.5586/asbp.2013.031.

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Hydrogen sulfide (H2S) is an important signaling molecule involved in several stress-resistance processes in plants, such as drought and heavy metal stresses. However, little is known about the roles of H2S in responses to chilling stress. In this paper, we demonstrated that chilling stress enhance the H2S levels, the H2S synthetase (L-/D-cysteine desulfhydrase, L/DCD) activities, and the expression of L/DCD gene in Vitis vinifera L. ‘F-242’. Furthermore, the seedlings were treated with sodium hydrosulﬁde (NaHS, a H2S donor) and hypotaurine (HT, a H2S scavenger) at 4°C to examine the effects of exogenous H2S on grape. The results revealed that the high activity of superoxide dismutase and enhanced expression of VvICE1 and VvCBF3 genes, but low level of super oxide anion radical, malondialdehyde content and cell membrane permeability were detected after addition of NaHS. In contrast, HT treatment displayed contrary effect under the chilling temperature. Taken together, these data suggested that H2S might be directly involved in the cold signal transduction pathway of grape.

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Deng, Chenglin, Chuanhai Deng, Han Wang, Jianying Zhong und Xinjing Cai. „Study on Two Residual Flux Suppression Strategies of Current Transformer During Automatic Reclosing“. Journal of Physics: Conference Series 2224, Nr. 1 (01.04.2022): 012054. http://dx.doi.org/10.1088/1742-6596/2224/1/012054.

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Abstract After power system short-circuit fault occurs, there is a large amount of residual magnetism in current transformer core, which will lead to wrong operation of connected relay protection and affect its reliability. Based on the direct current method, this paper puts forward two kinds of current transformer residual flux suppression strategies, which are constant voltage variable frequency (CVVF) and variable voltage constant frequency (VVCF), the magnetic flux of the core is gradually reduced by controlling the polarity of the output voltage of the switch module. The principle of residual flux suppression is introduced, and the method of parameter selection is given. The suppression effect of two residual flux suppression strategy is simulated and analyzed by experiment simulation. The results show that the two residual flux suppression strategies can demagnetize the current transformer core during automatic reclosing, but the constant voltage CVVF residual flux suppression strategy is simpler and more efficient.

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Lu, Ying, Xiaojie Ji und Yu Shu. „Method to Correct the Velocity Variation Information of an Automatic Crash Notification System in Vehicle-to-Rigid Barrier Frontal Collisions“. Shock and Vibration 2021 (18.08.2021): 1–18. http://dx.doi.org/10.1155/2021/5597886.

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Automatic crash notification systems (ACNSs) play a key role in post-accident safety. To improve the accuracy and efficiency of ACNSs, a method to correct the velocity variation information of ACNSs was established. First, after the acceleration data of sled crash tests were analysed, the factors affecting the accuracy of the velocity variation information were determined, and the influence of the discrimination threshold and acceleration curve shape on the velocity variation information was examined. Second, according to the acceleration data generated by the simulation model of a sled crash, the correlation between the accuracy of the velocity variation information and influencing factors was modelled. Third, an automatic crash notification algorithm involving a velocity variation correction function (VVCF) was proposed based on the correlation model. Finally, to verify its reliability, the improved algorithm was applied to an automatic crash notification system (ACNS) terminal. The validation results show that the ACNS terminal can accurately identify collisions and transmit accident information. Moreover, more accurate velocity variation information can be retrieved.

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Negara, I. Made Yulistya, Dicky Wahyu Darmawan, Ryan Nurdianto, Dedet Candra Riawan, Arif Musthofa, Dimas Anton Asfani, Mochammad Wahyudi und Daniar Fahmi. „Demagnetization Method for Reducing Inrush Current of Single Phase 1 kVA Transformer“. JAREE (Journal on Advanced Research in Electrical Engineering) 1, Nr. 1 (12.06.2017). http://dx.doi.org/10.12962/j25796216.v1.i1.4.

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This study deals with an effort to reduce inrush current of single phase transformer during its energizing. Inrush current affected by residual flux was minimized using demagnetization method in order to reduce residual flux trapped in the core of transformer. Two methods of demagnetization namely variable frequency-constant voltage (VFCV) and variablevoltage–constant frequency (VVCF) were applied and the effectiveness of both methods were then compared. Both methods were supplied with alternated direct current (DC), however the one was changed its frequency and the other one was changed its voltage. The results obtained after transformer was demagnetized using these methods show that VFCV method educed inrush current up to 76%, on the other hand VVCF ethod reduced inrush current only up to 37%. Moreover,demagnetization time process of VFCV method was about 1.46 s, which is 1 s faster than VVCF method.

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Leng, Xiangpeng, Chen Li, Peipei Wang, Yiran Ren, Jianqing Chen, Guangchao Liu, Abdul Hakeem et al. „The Transcription Factor VvMYB44-1 Plays a Role in Reducing Grapevine Anthocyanin Biosynthesis at High Temperature“. Plant Physiology, 11.12.2024. https://doi.org/10.1093/plphys/kiae657.

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Abstract High temperature reduces anthocyanin accumulation in various horticultural plants. However, the molecular mechanisms underlying the high-temperature-induced reduction of anthocyanin in grape (Vitis vinifera) remain poorly understood. In this study, VvMYB44-1 was identified as a transcriptional repressor of anthocyanin biosynthesis in grape berries, and its gene expression was strongly induced by high-temperature treatment. Overexpression of VvMYB44-1 inhibited anthocyanin accumulation in both grape berries and tobacco (Nicotiana tabacum) by repressing the transcription of the anthocyanin biosynthesis genes dihydroflavonol-4-reductase (VvDFR) and UDP-glucose flavonoid-3-O glucosyltransferase (VvUFGT).. Furthermore, the interaction between VvMYB44-1 and VvWDR2 competitively inhibited the formation of the MYB-bHLH-WD40 (MBW) activation complex and weakened the transcriptional activity of the complex, thereby decreasing anthocyanin accumulation. Additionally, VvMYB44-1 facilitated cytokinin accumulation by up-regulating the expression of the cytokinin synthesis gene lonely guy 8 (VvLOG8) and inhibiting the cytokinin degradation gene cytokinin oxidase 4(VvCKX4), thus contributing to cytokinin-mediated anthocyanin inhibition in grape berries. Moreover, the inhibitory effect of VvMYB44-1 on anthocyanin biosynthesis and its downstream target genes was weakened with the deletion of the ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif, indicating that the EAR motif is indispensable for the inhibitory effect of VvMYB44-1 on anthocyanin biosynthesis in grapes. These results provide insights into the regulatory network of VvMYB44-1 in high-temperature-mediated anthocyanin biosynthesis in grapes.

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Vergara, Ricardo, Ximena Noriega und Francisco J. Pérez. „VvDAM-SVPs genes are regulated by FLOWERING LOCUS T (VvFT) and not by ABA/low temperature-induced VvCBFs transcription factors in grapevine buds“. Planta 253, Nr. 2 (12.01.2021). http://dx.doi.org/10.1007/s00425-020-03561-5.

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Jang, Hyun A., May Moe Oo, Dae-Gyu Kim, Ha-Yeon Yoon, Mi-Reu Kim, Kyo-Sang Lee, Khaing Shwe Zin Thinn et al. „CC-NBS-LRR, a set of VvCRP markers, can distinguish cultivars with ripe rot resistance to Colletotrichum pathogens in grapevine“. Horticulture, Environment, and Biotechnology, 07.09.2020. http://dx.doi.org/10.1007/s13580-020-00290-2.

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Kondo, Satoru, Miho Ishioka, Chihiro Hoshi, Hiroyuki Tomiyama, Yukino Masuda, Souma Murata, Takanori Saito et al. „Effects of blue-light irradiation on abscisic acid signaling and sugar translocation in Vitis labruscana L.H. Bailey grapevines“. Plant Growth Regulation, 22.08.2024. http://dx.doi.org/10.1007/s10725-024-01206-1.

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AbstractThe effects of blue-light irradiation on abscisic acid (ABA) signaling, sugar metabolism and translocation, and photoreceptors and gene expressions were investigated to clarify the mechanism by which blue-LED irradiation increases sugar concentrations in grape berries (Vitis labruscana L.). Blue light-emitting diode (LED) irradiation increased the portion of 13C-photosynthates in the grapevine clusters that were fed 13CO2; compared to the portion in the cluster in the untreated control. Fructose and glucose concentrations and the expressions of VvSWEET10, VvSUC11, and VvSUS4 in blue LED-irradiated berries were increased. The blue LED-irradiated berries’ sucrose concentrations were significantly lower than the untreated control at 14 days after treatment. We speculated that the blue LED-treated berries’ decreased sucrose was associated with the increased Sugars Will Eventually be Exported Transporter (VvSWEET10), sucrose transporter (VvSUC11), and sucrose synthase (VvSUS4) expressions and promoted the translocation of 13C-photosynthates from the leaves that were fed 13CO2. Blue-LED irradiation increased the expressions of SNF1-related protein kinases (VvSnRK2.6) and ABA responding element binding transcription factor (VvABF1), while decreasing the expression of protein phosphateses 2C9 (VvPP2C9) genes, which are related to ABA signaling. Blue-LED irradiation increased the expressions of cryptochrome (VvCRYa) and phototropin (VvPHOT2), which are photoreceptor genes. The application of the pyrabactin resistance-like (PYL)-PP2C ABA receptor interaction antagonist AS6 did not affect endogenous ABA concentrations in the grape berries, but it decreased sucrose concentrations at harvest. The application of ABA did not affect sucrose, glucose, or fructose concentrations or the expressions of VvSnRK2.6 and VvPP2C9. The application of nordihydroguaiaretic acid (NDGA, an inhibitor of 9-cis-epoxycarotenoid dioxygenase activity in ABA biosynthesis) did not affect sugar concentrations at harvest. These results suggest that upregulation of photoreceptor gene expressions and ABA signaling are associated with sugar concentrations in grape berries.

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