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Thematische Bibliographien / Transfert de Lipides / Zeitschriftenartikel

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Zeitschriftenartikel zum Thema „Transfert de Lipides“

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Veröffentlicht am 30. November 2024

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1

Rougé, P., J. P. Borges, R. Culerrier, C. Brulé, A. Didier und A. Barre. „Les protéines de transfert des lipides : des allergènes importants des fruits“. Revue Française d'Allergologie 49, Nr. 2 (März 2009): 58–61. http://dx.doi.org/10.1016/j.reval.2009.01.003.

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Ghanem, R., A. Bouraoui, M. Berchel, T. Le Gall, O. Lozach, P. A. Jaffrès und T. Montier. „Lipides cationiques ramifiés pour le transfert de gènes par aérosol appliqué à la mucoviscidose“. Revue des Maladies Respiratoires 38, Nr. 6 (Juni 2021): 584–85. http://dx.doi.org/10.1016/j.rmr.2021.02.035.

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HAFFRAY, P., C. PINCENT, P. RAULT und B. COUDURIER. „Domestication et amélioration génétique des cheptels piscicoles français dans le cadre du SYSAAF“. INRAE Productions Animales 17, Nr. 3 (29.07.2004): 243–52. http://dx.doi.org/10.20870/productions-animales.2004.17.3.3598.

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Depuis 1991, des entreprises françaises sélectionnent la truite arc-en-ciel, la truite fario, le bar, la daurade et le turbot. Les éleveurs ont développé une expertise collective en matière de génétique quantitative, de contrôle de parenté assisté par empreintes génétiques, de monosexage, d’induction hormonale de la ponte, de triploïdisation et de conservation et congélation des gamètes, dans le cadre du Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF). Cette domestication s’appuie sur le transfert de la procédure de sélection sur la croissance PROSPER, élaborée par l’INRA, complétée de critères de sélection sur la qualité des carcasses (teneur en lipides des filets, rendement à l’éviscération) et d’une reproduction généalogique assistée par empreintes génétiques. L’amélioration des carcasses et de la chair est aussi réalisée par monosexage et triploïdisation. Depuis 2000, le transfert de la congélation du sperme est effectif. En 2003, entre 80 % et 100 % de la production française bénéficie de juvéniles provenant de programmes de sélection.

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MOULOUNGUI, Z., E. LACROUX, C. VACA-GARCIA und J. PEYDECASTAING. „Destruction des farines animales : valorisation des fractions lipidiques en biolubrifiants et additifs biocarburants, et du résidu protéique (ou de l’ensemble)“. INRAE Productions Animales 17, HS (20.12.2004): 117–22. http://dx.doi.org/10.20870/productions-animales.2004.17.hs.3637.

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L’objectif des travaux est d’étudier la faisabilité de nouvelles voies d’élimination et de valorisation des farines animales. Nous abordons ici l’étude de deux voies de valorisation complémentaires et indépendantes: d’une part, la valorisation de la fraction lipidique en «biocarburant» et «biolubrifiant» et, d’autre part, celle du résidu délipidé ou de l’ensemble de la matrice dans la fabrication de matériaux polymères. Dans un premier temps, les études développées se proposent de mettre en oeuvre un suivi qualité selon les recommandations de la Direction Générale de l’Alimentation. Ensuite, l’étude de la mise en oeuvre d’outils d’extraction à haut débit et de méthodes rapides de caractérisation des constituants majeurs et mineurs a pour but de caractériser la matrice «farines animales». La connaissance de la composition de la matrice a gouverné l’orientation de la stratégie de transformation de la fraction lipidique axée sur les études de transfert d’acyles. Elle permet d’obtenir des acides gras à partir des lipides extraits, puis de les utiliser pour synthétiser des esters gras simples. En perspective, nous proposons d’associer cette méthodologie à des technologies spécifiques qui permettront d’envisager la décontamination des farines animales lors de leur transformation. La deuxième partie des travaux consiste à utiliser les farines animales ainsi que les farines obtenues après extraction des lipides comme matières premières dans le cadre d’une étude de faisabilité concernant de nouveaux matériaux polymères. Il s’agit de procéder à la déstructuration thermochimique des macromolécules animales (susceptible de dégrader l’agent pathogène) dans le but de les réduire à l’état de monomères destinés à participer à la synthèse d’un nouveau polymère. Les essais de liquéfaction de cette matrice en présence de phénol ont donné des résultats encourageants. La fabrication de matériaux polymères constituerait une voie de valorisation permettant d’écouler de grandes quantités de farines animales.

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Pellerin-Massicotte, Jocelyne, Bruno Vincent und Émilien Pelletier. „Évaluation écotoxicologique de la baie des Anglais à Baie-Comeau (Québec)“. Water Quality Research Journal 28, Nr. 4 (01.11.1993): 665–86. http://dx.doi.org/10.2166/wqrj.1993.035.

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Résumé La baie des Anglais à Baie-Comeau (Québec) est un site industriel reconnu comme étant contaminé aux hydrocarbures et aux biphényls polychlorés (BPC). Une expérience de transfert à moyen terme de deux bivalves marins, Mya arenaria et Mytilus edulis L., a été réalisée entre un site de référence en aval de la baie des Anglais (Franquelin) et des sites contaminés près de Baie-Comeau suivant un gradient de contamination déterminé selon des données physico-chimiques antérieures. Les analyses chimiques de contaminants ont montré qu’il n’y a pas eu d’enrichissement en hydrocarbures, au mercure et en BPC pour toute la durée du protocole mais, parmi les sondes bioanalytiques choisies pour évaluer l’état de santé de cet écosystème, celles qui se sont avérées les plus sensibles chez Mya arenaria furent le glycogène et les lipides dans les gonades, et pour les deux bivalves, la fragilité de la membrane lysosomale de la glande digestive qui est un excellent indicateur de stress toxique. Les présents résultats sont compatibles avec un modèle qui consisterait à établir une évaluation ecotoxicologique d’un écosystème que l’on soupçonne perturbé par la pollution par (i) l’analyse de la bioaccumulation des substances toxiques que l’on croit présentes dans l’écosystème (hydrocarbures, BPC et métaux lourds) et (ii) l’évaluation des effets physiologiques et biochimiques des polluants à l’aide de sondes bioanalytiques appropriées.

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Levine, Tim P. „A lipid transfer protein that transfers lipid“. Journal of Cell Biology 179, Nr. 1 (08.10.2007): 11–13. http://dx.doi.org/10.1083/jcb.200709055.

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Very few lipid transfer proteins (LTPs) have been caught in the act of transferring lipids in vivo from a donor membrane to an acceptor membrane. Now, two studies (Halter, D., S. Neumann, S.M. van Dijk, J. Wolthoorn, A.M. de Maziere, O.V. Vieira, P. Mattjus, J. Klumperman, G. van Meer, and H. Sprong. 2007. J. Cell Biol. 179:101–115; D'Angelo, G., E. Polishchuk, G.D. Tullio, M. Santoro, A.D. Campli, A. Godi, G. West, J. Bielawski, C.C. Chuang, A.C. van der Spoel, et al. 2007. Nature. 449:62–67) agree that four-phosphate adaptor protein 2 (FAPP2) transfers glucosylceramide (GlcCer), a lipid that takes an unexpectedly circuitous route.

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Valverde, Diana P., Shenliang Yu, Venkata Boggavarapu, Nikit Kumar, Joshua A. Lees, Thomas Walz, Karin M. Reinisch und Thomas J. Melia. „ATG2 transports lipids to promote autophagosome biogenesis“. Journal of Cell Biology 218, Nr. 6 (05.04.2019): 1787–98. http://dx.doi.org/10.1083/jcb.201811139.

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During macroautophagic stress, autophagosomes can be produced continuously and in high numbers. Many different organelles have been reported as potential donor membranes for this sustained autophagosome growth, but specific machinery to support the delivery of lipid to the growing autophagosome membrane has remained unknown. Here we show that the autophagy protein, ATG2, without a clear function since its discovery over 20 yr ago, is in fact a lipid-transfer protein likely operating at the ER–autophagosome interface. ATG2A can bind tens of glycerophospholipids at once and transfers lipids robustly in vitro. An N-terminal fragment of ATG2A that supports lipid transfer in vitro is both necessary and fully sufficient to rescue blocked autophagosome biogenesis in ATG2A/ATG2B KO cells, implying that regulation of lipid homeostasis is the major autophagy-dependent activity of this protein and, by extension, that protein-mediated lipid transfer across contact sites is a principal contributor to autophagosome formation.

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Daminelli, Elaine Nunes, Celso Spada, Arício Treitinger, Tatiane Vanessa Oliveira, Maria da Conceição Latrilha und Raul Cavalcante Maranhão. „Alterations in lipid transfer to High-Density Lipoprotein (HDL) and activity of paraoxonase-1 in HIV+ patients“. Revista do Instituto de Medicina Tropical de São Paulo 50, Nr. 4 (August 2008): 223–27. http://dx.doi.org/10.1590/s0036-46652008000400007.

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HIV+ patients often develop alterations of the plasma lipids that may implicate in development of premature coronary artery disease. High-density lipoprotein (HDL) has an important role in preventing atherogenesis and the aim of this study was to investigate aspects of HDL function in HIV+ patients. HIV+ patients (n = 48) and healthy control subjects (n = 45) of both sexes with similar age were studied. Twenty-five were not being treated with antiretroviral agents, 13 were under reverse transcriptase inhibitor nucleosidic and non-nucleosidic (NRTI+NNRTI) and 10 were under NRTI + protease inhibitors (NRTI+PI) treatment. Paraoxonase 1 (PON1) activity and the transfer of free and esterified cholesterol, tryglicerides and phospholipids from a lipidic nanoemulsion to HDL were analyzed. In comparison with healthy controls, HIV+ patients presented low PON-1 activity and diminished transfer of free cholesterol and tryglicerides. In contrast, phospholipid transfer was increased in those patients, whereas the transfer of cholesteryl esters was unchanged. NRTI+NNRTI increases the transfer of cholesteryl esters and triglycerides but in NRTI+PI there was no difference in respect to non-treated HIV+ patients. HDL from HIV+ patients has smaller antioxidant properties, as shown by lower PON-1 activity, and the transfer of lipids to this lipoprotein fraction is also altered, suggesting that HDL function is defective in those patients.

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Dougan, Stephanie K., Azucena Salas, Paul Rava, Amma Agyemang, Arthur Kaser, Jamin Morrison, Archana Khurana et al. „Microsomal triglyceride transfer protein lipidation and control of CD1d on antigen-presenting cells“. Journal of Experimental Medicine 202, Nr. 4 (08.08.2005): 529–39. http://dx.doi.org/10.1084/jem.20050183.

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Microsomal triglyceride transfer protein (MTP), an endoplasmic reticulum (ER) chaperone that loads lipids onto apolipoprotein B, also regulates CD1d presentation of glycolipid antigens in the liver and intestine. We show MTP RNA and protein in antigen-presenting cells (APCs) by reverse transcription–polymerase chain reaction and by immunoblotting of mouse liver mononuclear cells and mouse and human B cell lines. Functional MTP, demonstrated by specific triglyceride transfer activity, is present in both mouse splenocytes and a CD1d-positive mouse NKT hybridoma. In a novel in vitro transfer assay, purified MTP directly transfers phospholipids, but not triglycerides, to recombinant CD1d. Chemical inhibition of MTP lipid transfer does not affect major histocompatibility complex class II presentation of ovalbumin, but considerably reduces CD1d-mediated presentation of α-galactosylceramide (α-galcer) and endogenous antigens in mouse splenic and bone marrow–derived dendritic cells (DCs), as well as in human APC lines and monocyte-derived DCs. Silencing MTP expression in the human monocyte line U937 affects CD1d function, as shown by diminished presentation of α-galcer. We propose that MTP acts upstream of the saposins and functions as an ER chaperone by loading endogenous lipids onto nascent CD1d. Furthermore, our studies suggest that a small molecule inhibitor could be used to modulate the activity of NKT cells.

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Bianco, Mariachiara, Giovanni Ventura, Davide Coniglio, Antonio Monopoli, Ilario Losito, Tommaso R. I. Cataldi und Cosima D. Calvano. „Development of a New Binary Matrix for the Comprehensive Analysis of Lipids and Pigments in Micro- and Macroalgae Using MALDI-ToF/ToF Mass Spectrometry“. International Journal of Molecular Sciences 25, Nr. 11 (29.05.2024): 5919. http://dx.doi.org/10.3390/ijms25115919.

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While edible algae might seem low in fat, the lipids they contain are crucial for good health and preventing chronic diseases. This study introduces a binary matrix to analyze all the polar lipids in both macroalgae (Wakame—Undaria pinnatifida, Dulse—Palmaria palmata, and Nori—Porphyra spp.) and microalgae (Spirulina—Arthrospira platensis, and Chlorella—Chlorella vulgaris) using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The key lies in a new dual matrix made by combining equimolar amounts of 1,5-diaminonaphthalene (DAN) and 9-aminoacridine (9AA). This combination solves the limitations of single matrices: 9AA is suitable for sulfur-containing lipids and acidic phospholipids, while DAN excels as an electron-transfer secondary reaction matrix for intact chlorophylls and their derivatives. By employing the equimolar binary matrix, a wider range of algal lipids, including free fatty acids, phospholipids, glycolipids, pigments, and even rare arsenosugarphospholipids were successfully detected, overcoming drawbacks related to ion suppression from readily ionizable lipids. The resulting mass spectra exhibited a good signal-to-noise ratio at a lower laser fluence and minimized background noise. This improvement stems from the binary matrix’s ability to mitigate in-source decay effects, a phenomenon often encountered for certain matrices. Consequently, the data obtained are more reliable, facilitating a faster and more comprehensive exploration of algal lipidomes using high-throughput MALDI-MS/MS analysis.

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WETZER, Barbara, Gerardo BYK, Marc FREDERIC, Marc AIRIAU, Francis BLANCHE, Bruno PITARD und Daniel SCHERMAN. „Reducible cationic lipids for gene transfer“. Biochemical Journal 356, Nr. 3 (15.06.2001): 747. http://dx.doi.org/10.1042/0264-6021:3560747.

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WETZER, Barbara, Gerardo BYK, Marc FREDERIC, Marc AIRIAU, Francis BLANCHE, Bruno PITARD und Daniel SCHERMAN. „Reducible cationic lipids for gene transfer“. Biochemical Journal 356, Nr. 3 (08.06.2001): 747–56. http://dx.doi.org/10.1042/bj3560747.

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One of the main challenges of gene therapy remains the increase of gene delivery into eukaryotic cells. We tested whether intracellular DNA release, an essential step for gene transfer, could be facilitated by using reducible cationic DNA-delivery vectors. For this purpose, plasmid DNA was complexed with cationic lipids bearing a disulphide bond. This reduction-sensitive linker is expected to be reduced and cleaved in the reducing milieu of the cytoplasm, thus potentially improving DNA release and consequently transfection. The DNA–disulphide-lipid complexation was monitored by ethidium bromide exclusion, and the size of complexes was determined by dynamic light scattering. It was found that the reduction kinetics of disulphide groups in DNA–lipid complexes depended on the position of the disulphide linker within the lipid molecule. Furthermore, the internal structure of DNA–lipid particles was examined by small-angle X-ray scattering before and after lipid reduction. DNA release from lipid complexes was observed after the reduction of disulphide bonds of several lipids. Cell-transfection experiments suggested that complexes formed with selected reducible lipids resulted in up to 1000-fold higher reporter-gene activity, when compared with their analogues without disulphide bonds. In conclusion, reduction-sensitive groups introduced into cationic lipid backbones potentially allow enhanced DNA release from DNA–lipid complexes after intracellular reduction and represent a tool for improved vectorization.

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Zabner, J. „Cationic lipids used in gene transfer“. Advanced Drug Delivery Reviews 27, Nr. 1 (11.08.1997): 17–28. http://dx.doi.org/10.1016/s0169-409x(97)00019-7.

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Moreau, P., und D. J. Morré. „Cell-free transfer of membrane lipids.“ Journal of Biological Chemistry 266, Nr. 7 (März 1991): 4329–33. http://dx.doi.org/10.1016/s0021-9258(20)64326-5.

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Ismail, Vian, Burkhard Bechinger, Jackie A. Mosely und John M. Sanderson. „Peptide Lipidation by Acyl Transfer from Membrane Lipids and Lyso-Lipids“. Biophysical Journal 106, Nr. 2 (Januar 2014): 296a. http://dx.doi.org/10.1016/j.bpj.2013.11.1726.

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Tucker, Torry A., Karoly Varga, Zsuzsa Bebok, Akos Zsembery, Nael A. McCarty, James F. Collawn, Erik M. Schwiebert und Lisa M. Schwiebert. „Transient transfection of polarized epithelial monolayers with CFTR and reporter genes using efficacious lipids“. American Journal of Physiology-Cell Physiology 284, Nr. 3 (01.03.2003): C791—C804. http://dx.doi.org/10.1152/ajpcell.00435.2002.

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Transient transfection of epithelial cells with lipid reagents has been limited because of toxicity and lack of efficacy. In this study, we show that more recently developed lipids transfect nonpolarized human airway epithelial cells with high efficacy and efficiency and little or no toxicity. Because of this success, we hypothesized that these lipids may also allow transient transfection of polarized epithelial monolayers. A panel of reagents was tested for transfer of the reporter gene luciferase ( LUC) into polarized monolayers of non-cystic fibrosis (non-CF) and CF human bronchial epithelial cells, MDCK epithelial cell monolayers, and, ultimately, primary non-CF and CF airway epithelial cells. Lipid reagents, which were most successful in initial LUC assays, were also tested for transfer of vectors bearing the reporter gene green fluorescent protein (GFP) and for successful transfection and expression of an epithelial-specific protein, the cystic fibrosis transmembrane conductance regulator (CFTR). Electrophysiological, biochemical, and immunological assays were performed to show successful complementation of an epithelial monolayer with transiently expressed CFTR. We also present findings that help facilitate monolayer formation by these airway epithelial cell lines. Together, these data show that polarized monolayers are transfected transiently with more recently developed lipids, specifically LipofectAMINE PLUS and LipofectAMINE 2000. Transient transfection of epithelial monolayers provides a powerful system in which to express the cDNA of any epithelium-specific protein transiently in a native polarized epithelium to study protein function.

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Somerharju, Pentti. „Is Spontaneous Translocation of Polar Lipids between Cellular Organelles Negligible?“ Lipid Insights 8s1 (Januar 2015): LPI.S31616. http://dx.doi.org/10.4137/lpi.s31616.

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In most reviews addressing intracellular lipid trafficking, spontaneous diffusion of lipid monomers between the cellular organelles is considered biologically irrelevant because it is thought to be far too slow to significantly contribute to organelle biogenesis. This view is based on intervesicle transfer experiments carried out in vitro with few lipids as well as on the view that lipids are highly hydrophobic and thus cannot undergo spontaneous intermembrane diffusion at a significant rate. However, besides that single-chain lipids can translocate between vesicles in seconds, it has been demonstrated that the rate of spontaneous transfer of two-chain polar lipids can vary even 1000-fold, depending on the number of carbons and double bonds in the acyl chains. In addition, the rate of spontaneous lipid transfer can strongly depend on the experimental conditions such as vesicle composition and concentration. This review examines the studies suggesting that spontaneous lipid transfer is probably more relevant to intracellular trafficking of amphipathic lipids than commonly thought.

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Wong, Louise H., Alenka Čopič und Tim P. Levine. „Advances on the Transfer of Lipids by Lipid Transfer Proteins“. Trends in Biochemical Sciences 42, Nr. 7 (Juli 2017): 516–30. http://dx.doi.org/10.1016/j.tibs.2017.05.001.

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Otomo, Takanori, und Shintaro Maeda. „ATG2A transfers lipids between membranes in vitro“. Autophagy 15, Nr. 11 (26.08.2019): 2031–32. http://dx.doi.org/10.1080/15548627.2019.1659622.

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Klein, Emmanuel, Christian Leborgne, Miahala Ciobanu, Jérôme Klein, Benoît Frisch, Françoise Pons, Guy Zuber, Daniel Scherman, Antoine Kichler und Luc Lebeau. „Nucleic acid transfer with hemifluorinated polycationic lipids“. Biomaterials 31, Nr. 17 (Juni 2010): 4781–88. http://dx.doi.org/10.1016/j.biomaterials.2010.02.047.

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Zhang, Yongli, Jinghua Ge, Xin Bian und Avinash Kumar. „Quantitative Models of Lipid Transfer and Membrane Contact Formation“. Contact 5 (Januar 2022): 251525642210960. http://dx.doi.org/10.1177/25152564221096024.

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Lipid transfer proteins (LTPs) transfer lipids between different organelles, and thus play key roles in lipid homeostasis and organelle dynamics. The lipid transfer often occurs at the membrane contact sites (MCS) where two membranes are held within 10–30 nm. While most LTPs act as a shuttle to transfer lipids, recent experiments reveal a new category of eukaryotic LTPs that may serve as a bridge to transport lipids in bulk at MCSs. However, the molecular mechanisms underlying lipid transfer and MCS formation are not well understood. Here, we first review two recent studies of extended synaptotagmin (E-Syt)-mediated membrane binding and lipid transfer using novel approaches. Then we describe mathematical models to quantify the kinetics of lipid transfer by shuttle LTPs based on a lipid exchange mechanism. We find that simple lipid mixing among membranes of similar composition and/or lipid partitioning among membranes of distinct composition can explain lipid transfer against a concentration gradient widely observed for LTPs. We predict that selective transport of lipids, but not membrane proteins, by bridge LTPs leads to osmotic membrane tension by analogy to the osmotic pressure across a semipermeable membrane. A gradient of such tension and the conventional membrane tension may drive bulk lipid flow through bridge LTPs at a speed consistent with the fast membrane expansion observed in vivo. Finally, we discuss the implications of membrane tension and lipid transfer in organelle biogenesis. Overall, the quantitative models may help clarify the mechanisms of LTP-mediated MCS formation and lipid transfer.

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Parrish, Christopher C. „Lipids in Marine Ecosystems“. ISRN Oceanography 2013 (22.04.2013): 1–16. http://dx.doi.org/10.5402/2013/604045.

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Lipids provide the densest form of energy in marine ecosystems. They are also a solvent and absorption carrier for organic contaminants and thus can be drivers of pollutant bioaccumulation. Among the lipids, certain essential fatty acids and sterols are considered to be important determinants of ecosystem health and stability. Fatty acids and sterols are also susceptible to oxidative damage leading to cytotoxicity and a decrease in membrane fluidity. The physical characteristics of biological membranes can be defended from the influence of changing temperature, pressure, or lipid peroxidation by altering the fatty acid and sterol composition of the lipid bilayer. Marine lipids are also a valuable tool to measure inputs, cycling, and loss of materials. Their heterogeneous nature makes them versatile biomarkers that are widely used in marine trophic studies, often with the help of multivariate statistics, to delineate carbon cycling and transfer of materials. Principal components analysis has a strong following as it permits data reduction and an objective interpretation of results, but several more sophisticated multivariate analyses which are more quantitative are emerging too. Integrating stable isotope and lipid data can facilitate the interpretation of both data sets and can provide a quantitative estimate of transfer across trophic levels.

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Le Gall, Tony, Mathieu Berchel, Lee Davies, Angélique Mottais, Rosy Ghanem, Alain Fautrel, Deborah Gill et al. „Aerosol-Mediated Non-Viral Lung Gene Therapy: The Potential of Aminoglycoside-Based Cationic Liposomes“. Pharmaceutics 14, Nr. 1 (23.12.2021): 25. http://dx.doi.org/10.3390/pharmaceutics14010025.

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Aerosol lung gene therapy using non-viral delivery systems represents a credible therapeutic strategy for chronic respiratory diseases, such as cystic fibrosis (CF). Progress in CF clinical setting using the lipidic formulation GL67A has demonstrated the relevance of such a strategy while emphasizing the need for more potent gene transfer agents. In recent years, many novel non-viral gene delivery vehicles were proposed as potential alternatives to GL67 cationic lipid. However, they were usually evaluated using procedures difficult or even impossible to implement in clinical practice. In this study, a clinically-relevant administration protocol via aerosol in murine lungs was used to conduct a comparative study with GL67A. Diverse lipidic compounds were used to prepare a series of formulations inspired by the composition of GL67A. While some of these formulations were ineffective at transfecting murine lungs, others demonstrated modest-to-very-efficient activities and a series of structure-activity relationships were unveiled. Lipidic aminoglycoside derivative-based formulations were found to be at least as efficient as GL67A following aerosol delivery of a luciferase-encoding plasmid DNA. A single aerosol treatment with one such formulation was found to mediate long-term lung transgene expression, exceeding half the animal’s lifetime. This study clearly supports the potential of aminoglycoside-based cationic lipids as potent GL67-alternative scaffolds for further enhanced aerosol non-viral lung gene therapy for diseases such as CF.

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Goto, Asako, Aya Mizuike und Kentaro Hanada. „Sphingolipid Metabolism at the ER-Golgi Contact Zone and Its Impact on Membrane Trafficking“. Contact 3 (Januar 2020): 251525642095951. http://dx.doi.org/10.1177/2515256420959514.

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Proteins and lipids represent the two major constituents of biological membranes. Different organelles have different lipid compositions, which may be crucial for the execution and control of various organelle-specific functions. The interorganellar transport of lipids is dominated by mechanisms that are distinct from the vesicular mechanisms that underlie the interorganellar transport of proteins. Lipid transfer proteins (LTPs) efficiently and accurately mediate the trafficking of membrane lipids at the interfaces between different organelles. In this review, which focuses on sphingolipids, we describe the coordinated synthesis and transfer of lipids that occur at the endoplasmic reticulum (ER)-Golgi apparatus contact zones and discuss the impacts of lipid metabolism on membrane trafficking from the trans-Golgi network (TGN).

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Bourgis, Fabienne, und Jean-Claude Kader. „Lipid-transfer proteins: Tools for manipulating membrane lipids“. Physiologia Plantarum 100, Nr. 1 (Mai 1997): 78–84. http://dx.doi.org/10.1111/j.1399-3054.1997.tb03456.x.

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Bourgis, Fabienne, und Jean-Claude Kader. „Lipid-transfer proteins: Tools for manipulating membrane lipids“. Physiologia Plantarum 100, Nr. 1 (Mai 1997): 78–84. http://dx.doi.org/10.1034/j.1399-3054.1997.1000107.x.

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Burlakova, E. B. „Membrane lipids in transfer and storage of information“. Neurochemistry International 21 (Januar 1992): A13. http://dx.doi.org/10.1016/0197-0186(92)91943-q.

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Bagdade, J. D., M. Ritter und P. V. Subbaiah. „Marine lipids normalize cholesteryl ester transfer in IDDM“. Diabetologia 39, Nr. 4 (01.03.1996): 487–91. http://dx.doi.org/10.1007/s001250050470.

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Bagdade, J. D., M. Ritter und P. V. Subbaiah. „Marine lipids normalize cholesteryl ester transfer in IDDM“. Diabetologia 39, Nr. 4 (April 1996): 487–91. http://dx.doi.org/10.1007/bf00400682.

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Ilies, Marc Antoniu, William A. Seitz, Miron T. Caproiu, Melissa Wentz, Robert E. Garfield und Alexandru T. Balaban. „Pyridinium-Based Cationic Lipids as Gene-Transfer Agents“. European Journal of Organic Chemistry 2003, Nr. 14 (Juli 2003): 2645–55. http://dx.doi.org/10.1002/ejoc.200300106.

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Liang, Xiaorong, Jian Liu, Yves LeBlanc, Tom Covey, A. Celeste Ptak, J. Thomas Brenna und Scott A. McLuckey. „Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids“. Journal of the American Society for Mass Spectrometry 18, Nr. 10 (Oktober 2007): 1783–88. http://dx.doi.org/10.1016/j.jasms.2007.07.013.

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Lee, Robert J., und Leaf Huang. „Lipidic Vector Systems for Gene Transfer“. Critical Reviews in Therapeutic Drug Carrier Systems 14, Nr. 2 (1997): 34. http://dx.doi.org/10.1615/critrevtherdrugcarriersyst.v14.i2.30.

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Li, Song, und Leaf Huang. „Lipidic Supramolecular Assemblies for Gene Transfer“. Journal of Liposome Research 6, Nr. 3 (Januar 1996): 589–608. http://dx.doi.org/10.3109/08982109609031138.

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Zhao, Yinan, Shubiao Zhang, Yuan Zhang, Shaohui Cui, Huiying Chen, Defu Zhi, Yuhong Zhen, Shufen Zhang und Leaf Huang. „Tri-peptide cationic lipids for gene delivery“. Journal of Materials Chemistry B 3, Nr. 1 (2015): 119–26. http://dx.doi.org/10.1039/c4tb01312c.

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Mukherjee, Koushik, Joyeeta Sen und Arabinda Chaudhuri. „Common co-lipids, in synergy, impart high gene transfer properties to transfection-incompetent cationic lipids“. FEBS Letters 579, Nr. 5 (26.01.2005): 1291–300. http://dx.doi.org/10.1016/j.febslet.2004.11.116.

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NAKANISHI, Toshiaki, Toshiro NISHIDA, Yoshihiko YAMAGUCHI, Yasuo UEDA, Atsushi YOKOTA, Tsutomu MORI, Shoichi AKAZAWA, Seibei MIYAKE und Shigenobu NAGATAKI. „Apolipoprotein D: Isolation and Effect on Lipid Transfer Activity“. Journal of Japan Atherosclerosis Society 15, Nr. 4 (1987): 945–49. http://dx.doi.org/10.5551/jat1973.15.4_945.

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Saito, Hiroshi, Hidenori Nakamura, Suichi Kato, Sumito Inoue, Minoru Inage, Minoru Ito und Hitonobu Tomoike. „Percutaneous in vivo gene transfer to the peripheral lungs using plasmid-liposome complexes“. American Journal of Physiology-Lung Cellular and Molecular Physiology 279, Nr. 4 (01.10.2000): L651—L657. http://dx.doi.org/10.1152/ajplung.2000.279.4.l651.

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The purpose of this study was to investigate a new method of in vivo gene transfer to the lung parenchyma by the percutaneous approach. The plasmid that contains the gene for firefly luciferase driven by a cytomegalovirus (CMV) promoter (pCMVL) in combination with cationic lipids was percutaneously injected into the lung parenchyma. Luciferase activities were localized to the lobes of the lung where the plasmids with cationic lipids were injected. Percutaneous injection of the plasmid containing the human endothelin-1 (hET-1) gene driven by a CMV promoter (pRc/CMVhET-1) in combination with cationic lipids into the lungs caused pulmonary fibrosis localized to the injection site in the peripheral lungs. We concluded that percutaneous in vivo gene transfer to the lungs is a unique and important approach to introduce exogenous gene expression in the limited area of the lung parenchyma. This method of gene transfer will be applicable for human gene therapy for targeted areas of peripheral lung and will also be useful to assess the function of the proteins expressed by a gene in the local area of the lungs.

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Rossi, Sergio, Enrique Isla, Alfredo Martínez-García, Núria Moraleda, Josep María Gili, Antoni Rosell-Melé, Wolf E. Arntz und Dieter Gerdes. „Transfer of seston lipids during a flagellate bloom from the surface to the benthic community in the Weddell Sea“. Scientia Marina 77, Nr. 3 (12.09.2013): 397–407. http://dx.doi.org/10.3989/scimar.03835.30a.

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Nakano, Minoru. „Determination of Intervesicular Transfer of Lipids by SANS-U“. hamon 18, Nr. 2 (2008): 79–82. http://dx.doi.org/10.5611/hamon.18.79.

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Ouali, Mustapha, Jean-Marie Ruysschaert, Caroline Lonez und Michel Vandenbranden. „Cationic lipids involved in gene transfer mobilize intracellular calcium“. Molecular Membrane Biology 24, Nr. 3 (Januar 2007): 225–32. http://dx.doi.org/10.1080/09687860601108911.

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Gascón, Alicia R., und Jose Luis Pedraz. „Cationic lipids as gene transfer agents: a patent review“. Expert Opinion on Therapeutic Patents 18, Nr. 5 (Mai 2008): 515–24. http://dx.doi.org/10.1517/13543776.18.5.515.

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Yu, Haijia, Yinghui Liu, Daniel R. Gulbranson, Alex Paine, Shailendra S. Rathore und Jingshi Shen. „Extended synaptotagmins are Ca2+-dependent lipid transfer proteins at membrane contact sites“. Proceedings of the National Academy of Sciences 113, Nr. 16 (04.04.2016): 4362–67. http://dx.doi.org/10.1073/pnas.1517259113.

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Organelles are in constant communication with each other through exchange of proteins (mediated by trafficking vesicles) and lipids [mediated by both trafficking vesicles and lipid transfer proteins (LTPs)]. It has long been known that vesicle trafficking can be tightly regulated by the second messenger Ca2+, allowing membrane protein transport to be adjusted according to physiological demands. However, it remains unclear whether LTP-mediated lipid transport can also be regulated by Ca2+. In this work, we show that extended synaptotagmins (E-Syts), poorly understood membrane proteins at endoplasmic reticulum–plasma membrane contact sites, are Ca2+-dependent LTPs. Using both recombinant and endogenous mammalian proteins, we discovered that E-Syts transfer glycerophospholipids between membrane bilayers in the presence of Ca2+. E-Syts use their lipid-accommodating synaptotagmin-like mitochondrial lipid binding protein (SMP) domains to transfer lipids. However, the SMP domains themselves cannot transport lipids unless the two membranes are tightly tethered by Ca2+-bound C2 domains. Strikingly, the Ca2+-regulated lipid transfer activity of E-Syts was fully recapitulated when the SMP domain was fused to the cytosolic domain of synaptotagmin-1, the Ca2+ sensor in synaptic vesicle fusion, indicating that a common mechanism of membrane tethering governs the Ca2+ regulation of lipid transfer and vesicle fusion. Finally, we showed that microsomal vesicles isolated from mammalian cells contained robust Ca2+-dependent lipid transfer activities, which were mediated by E-Syts. These findings established E-Syts as a novel class of LTPs and showed that LTP-mediated lipid trafficking, like vesicular transport, can be subject to tight Ca2+ regulation.

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Tamura, Yasushi, Shin Kawano und Toshiya Endo. „Lipid homeostasis in mitochondria“. Biological Chemistry 401, Nr. 6-7 (26.05.2020): 821–33. http://dx.doi.org/10.1515/hsz-2020-0121.

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AbstractMitochondria are surrounded by the two membranes, the outer and inner membranes, whose lipid compositions are optimized for proper functions and structural organizations of mitochondria. Although a part of mitochondrial lipids including their characteristic lipids, phosphatidylethanolamine and cardiolipin, are synthesized within mitochondria, their precursor lipids and other lipids are transported from other organelles, mainly the ER. Mitochondrially synthesized lipids are re-distributed within mitochondria and to other organelles, as well. Recent studies pointed to the important roles of inter-organelle contact sites in lipid trafficking between different organelle membranes. Identification of Ups/PRELI proteins as lipid transfer proteins shuttling between the mitochondrial outer and inner membranes established a part of the molecular and structural basis of the still elusive intra-mitochondrial lipid trafficking.

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Britt, Hannah M., Jackie A. Mosely und John M. Sanderson. „The influence of cholesterol on melittin lipidation in neutral membranes“. Physical Chemistry Chemical Physics 21, Nr. 2 (2019): 631–40. http://dx.doi.org/10.1039/c8cp06661b.

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Toyoda, Kazunori, Hiroshi Nakane und Donald D. Heistad. „Cationic Polymer and Lipids Augment Adenovirus-Mediated Gene Transfer to Cerebral Arteries In vivo“. Journal of Cerebral Blood Flow & Metabolism 21, Nr. 9 (September 2001): 1125–31. http://dx.doi.org/10.1097/00004647-200109000-00010.

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Adenovirus-mediated gene transfer to blood vessels is relatively inefficient because binding of adenovirus to vessels is limited. The authors have reported that incorporation of cationic polymer and lipids with adenovirus augments gene transfer to blood vessels ex vivo. In this study, the authors determined whether complexes of adenovirus and cations improve efficiency of gene transfer in vivo. Poly-l-lysine, lipofectamine, or lipofectin was complexed with adenovirus encoding β-galactosidase. Optimum ratios of the cations per adenovirus were determined by gene transfer to fibroblasts. After injection of the adenovirus into the cisterna magna of anesthetized rabbits, transgene activity was greater in the adventitia of intracranial arteries and meninges after injection of the complexes than adenovirus alone. Thirty minutes after application of adenovirus with the cations, binding of adenovirus to fibroblast cells in vitro or the basilar artery in vivo (by Southern blot analysis) was augmented, which suggests that enhanced binding of virus contributes to augmentation of transgene expression. Thus, cationic polymer and lipids improve transgene expression in intracranial arteries, primarily in the adventitia, after adenovirus-mediated gene transfer in vivo. This strategy may be applicable to studies of gene transfer and eventually for gene therapy.

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Schrantz, Nicolas, Yuval Sagiv, Yang Liu, Paul B. Savage, Albert Bendelac und Luc Teyton. „The Niemann-Pick type C2 protein loads isoglobotrihexosylceramide onto CD1d molecules and contributes to the thymic selection of NKT cells“. Journal of Experimental Medicine 204, Nr. 4 (26.03.2007): 841–52. http://dx.doi.org/10.1084/jem.20061562.

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The Niemann-Pick type C2 (NPC2) protein is a small, soluble, lysosomal protein important for cholesterol and sphingolipid transport in the lysosome. The immunological phenotype of NPC2-deficient mice was limited to an impaired thymic selection of Vα14 natural killer T cells (NKT cells) and a subsequent reduction of NKT cells in the periphery. The remaining NKT cells failed to produce measurable quantities of interferon-γ in vivo and in vitro after activation with α-galactosylceramide. In addition, thymocytes and splenocytes from NPC2-deficient mice were poor presenters of endogenous and exogenous lipids to CD1d-restricted Vα14 hybridoma cells. Importantly, we determined that similar to saposins, recombinant NPC2 was able to unload lipids from and load lipids into CD1d. This transfer activity was associated with a dimeric form of NPC2, suggesting a unique mechanism of glycosphingolipid transfer by NPC2. Similar to saposin B, NPC2 dimers were able to load isoglobotrihexosylceramide (iGb3), the natural selecting ligand of NKT cells in the thymus, into CD1d. These observations strongly suggested that the phenotype observed in NPC2-deficient animals was directly linked to the efficiency of the loading of iGb3 into CD1d molecules expressed by thymocytes. This conclusion was supported by the rescue of endogenous and exogenous iGb3 presentation by recombinant NPC2. Thus, the loading of endogenous and exogenous lipids and glycolipids onto CD1d is dependent on various small, soluble lipid transfer proteins present in the lysosome.

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Ghanbarpour, Alireza, Diana P. Valverde, Thomas J. Melia und Karin M. Reinisch. „A model for a partnership of lipid transfer proteins and scramblases in membrane expansion and organelle biogenesis“. Proceedings of the National Academy of Sciences 118, Nr. 16 (13.04.2021): e2101562118. http://dx.doi.org/10.1073/pnas.2101562118.

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The autophagy protein ATG2, proposed to transfer bulk lipid from the endoplasmic reticulum (ER) during autophagosome biogenesis, interacts with ER residents TMEM41B and VMP1 and with ATG9, in Golgi-derived vesicles that initiate autophagosome formation. In vitro assays reveal TMEM41B, VMP1, and ATG9 as scramblases. We propose a model wherein membrane expansion results from the partnership of a lipid transfer protein, moving lipids between the cytosolic leaflets of apposed organelles, and scramblases that reequilibrate the leaflets of donor and acceptor organelle membranes as lipids are depleted or augmented. TMEM41B and VMP1 are implicated broadly in lipid homeostasis and membrane dynamics processes in which their scrambling activities likely are key.

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NAKANISHI, Toshiaki, Toshiro NISHIDA, Daigo TAHARA, Shoichi AKAZAWA, Seibei MIYAKE und Shigenobu NAGATAKI. „Purification of Lipid Transfer Protein from Human Plasma and Preparation of Anti Human LTP IgG“. Journal of Japan Atherosclerosis Society 15, Nr. 8 (1988): 1631–37. http://dx.doi.org/10.5551/jat1973.15.8_1631.

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Chen, Sijin, Xiaoyan Ding, Chao Sun, Fei Wang, Xiao He, Anthony Watts und Xin Zhao. „Archaeal Lipids Regulating the Trimeric Structure Dynamics of Bacteriorhodopsin for Efficient Proton Release and Uptake“. International Journal of Molecular Sciences 23, Nr. 13 (21.06.2022): 6913. http://dx.doi.org/10.3390/ijms23136913.

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S-TGA-1 and PGP-Me are native archaeal lipids associated with the bacteriorhodopsin (bR) trimer and contribute to protein stabilization and native dynamics for proton transfer. However, little is known about the underlying molecular mechanism of how these lipids regulate bR trimerization and efficient photocycling. Here, we explored the specific binding of S-TGA-1 and PGP-Me with the bR trimer and elucidated how specific interactions modulate the bR trimeric structure and proton release and uptake using long-term atomistic molecular dynamic simulations. Our results showed that S-TGA-1 and PGP-Me are essential for stabilizing the bR trimer and maintaining the coherent conformational dynamics necessary for proton transfer. The specific binding of S-TGA-1 with W80 and K129 regulates proton release on the extracellular surface by forming a “Glu-shared” model. The interaction of PGP-Me with K40 ensures proton uptake by accommodating the conformation of the helices to recruit enough water molecules on the cytoplasmic side. The present study results could fill in the theoretical gaps of studies on the functional role of archaeal lipids and could provide a reference for other membrane proteins containing similar archaeal lipids.

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Finkina, E. I., D. N. Melnikova, I. V. Bogdanov und T. V. Ovchinnikova. „Lipid Transfer Proteins As Components of the Plant Innate Immune System: Structure, Functions, and Applications“. Acta Naturae 8, Nr. 2 (15.06.2016): 47–61. http://dx.doi.org/10.32607/20758251-2016-8-2-47-61.

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Among a variety of molecular factors of the plant innate immune system, small proteins that transfer lipids and exhibit a broad spectrum of biological activities are of particular interest. These are lipid transfer proteins (LTPs). LTPs are interesting to researchers for three main features. The first feature is the ability of plant LTPs to bind and transfer lipids, whereby these proteins got their name and were combined into one class. The second feature is that LTPs are defense proteins that are components of plant innate immunity. The third feature is that LTPs constitute one of the most clinically important classes of plant allergens. In this review, we summarize the available data on the plant LTP structure, biological properties, diversity of functions, mechanisms of action, and practical applications, emphasizing their role in plant physiology and their significance in human life.

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