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Auswahl der wissenschaftlichen Literatur zum Thema „The isolated fungi“
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Zeitschriftenartikel zum Thema "The isolated fungi"
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Shonde, A. T., A. A. Adekunle, T. O. Samuel, O. O. Adeogun, A. M. Ebabhi und U. C. Kanife. „Isolation, Identification and Effect of Fungi from Rhizosphere of Diseased Amaranthus hybridus L., Solanum lycopersicum L., Lactuca sativa L. and Allium fistulosum L Vegetable Crops in selected Farms in Lagos State, Nigeria“. Journal of Applied Sciences and Environmental Management 27, Nr. 5 (31.05.2023): 989–94. http://dx.doi.org/10.4314/jasem.v27i5.16.
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Soil microorganisms play prominent roles in soil health, promotion of crop growth and incidence of disease. The objective of this study was to isolate, identify and understand the impact of fungi isolated from rhizosphere and diseased Amaranthus hybridus L., Solanum lycopersicum L., Lactuca sativa L. and Allium fistulosum L vegetable crops in selected farms in Lagos State of Nigeria using standard techniques. A pathogenicity test was conducted to ascertain the pathogen responsible for crop diseases. A total of 138 fungi isolates comprising of 26 fungi species were isolated from the four selected farms. Idi-Araba farm had the highest fungi isolates with 46%, Mile 12 contributed 24% fungi isolates, Iyana Iba had 18% while the least contribution was Badagry farm with 12% fungi isolates. Five were found to be pathogenic on the vegetables which include Fusarium oxysporium, Fusarium solani, Macrophomina phaseolina, Alternaria alternata, and Colletotrichum gleosporoides. Thirty-three (33%) percent of pathogenic fungi isolated were from Idi-araba farm, Iyana-Iba and Mile 12 farms contributed 25% respectively while Badagry farm contributed 17% pathogenic fungi. Our results showed that the presence of pathogenic fungi in all farms is the principal cause of crop disease and severe post-harvest losses.
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Vidal, Araceli, Rodolfo Parada, Leonora Mendoza und Milena Cotoras. „Endophytic Fungi Isolated from Plants Growing in Central Andean Precordillera of Chile with Antifungal Activity against Botrytis cinerea“. Journal of Fungi 6, Nr. 3 (26.08.2020): 149. http://dx.doi.org/10.3390/jof6030149.
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Botrytis cinerea is an important phytopathogenic fungus affecting the fruit production around the world. This fungus is controlled mainly by using synthetic fungicides, but many resistant isolates have been selected by the indiscriminate use of fungicides. Endophytic fungi or secondary metabolites obtained from them become an alternative method of control for this fungus. The aim of this work was to identify endophytic fungi with antifungal activity against the plant pathogenic fungus B. cinerea isolated from plants from Central Andean Precordillera of Chile. Three endophytic fungi (Ac1, Lc1 and Ec1) with antifungal activity against B. cinerea were isolated from native and endemic plants growing in Central Andean Precordillera of Chile. The isolates Lc1 (isolated from Lithraea caustica) and Ac1 (isolated from Acacia caven) were identified as Alternaria spp. and the isolate Ec1 (isolated from Echinopsis chiloensis) was identified as Aureobasidium spp. The isolated endophytic fungi would inhibit B. cinerea through the secretion of diffusible and volatile compounds affecting the mycelial growth, conidia germination and interestingly, it was also shown that the volatile compounds produced by the three isolated endophytic fungi suppressed the sporulation of B. cinerea.
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Fajarningsih, Nurrahmi Dewi, Muhammad Nursid und Ekowati Chasanah. „Screening of Antitumor Bioactivity of Fungi Associated with Macro Algae and Sponge from Indrayanti Beach, Jogjakarta“. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 8, Nr. 2 (24.06.2014): 47. http://dx.doi.org/10.15578/squalen.v8i2.25.
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This research was aimed to isolate marine derived-fungi which associated with macro algae and sponge from Indrayanti Beach, Jogjakarta and to screen the antitumor (T47D and HeLa) bioactivity of the fungi extracts. Three solid media of MEA (malt extract agar), GPY (glucose peptone yeast) and MFM (minimal fungi medium) were used as isolation medium. Each of the pure fungi isolates was then cultivated in 100 ml of liquid medium for 4 weeks at room temperature (27-28°C) in static conditions. The antitumor activity of the fungi extracts were tested against breast tumor cells (T47D) and cervical cancer cells (HeLa) using Thiazolyl Blue Tetrazolium Blue (MTT) assay method. A number of 21 isolates of fungi were isolated from 4 macro algae and 1 sponge samples. The identification of fungi isolate was conducted using combination of molecular approach (ITS1-5.8S-ITS4 DNA regions) and macro-micro morphological characteristics. Among those 21 marine fungi species isolated, MFGK-21 extract showed the best anti-servical tumor (HeLa) with an IC50 value of 240.1 µg/ml and MFGK-27 extract showed the best anti-breast tumor (T47D) with an IC50 value of 59.6 µg/ml. The MFGK-21 fungi isolate was identified as Penicillium steckii, while the MFGK-27 fungi isolate was identified as Aspergillus sydowii.
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Suciatmih, Suaciatmih, Yuliar Yuliar und D. Supriyati. „ISOLASI, IDENTIFIKASI, DAN SKRINING JAMUR ENDOFIT PENGHASIL AGEN BIOKONTROL DARI TANAMAN DI LAHAN PERTANIAN DAN HUTAN PENUNJANG GUNUNG SALAK“. Jurnal Teknologi Lingkungan 12, Nr. 2 (01.12.2016): 171. http://dx.doi.org/10.29122/jtl.v12i2.1249.
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Isolation of endophytic fungi was done to find alternative microorganisms as biocontrol agents against Rhizoctonia solani Kuhn, a soil borne pathogen on many agriculturalcrops. The research objectives were 1) to isolate and identify endophytic fungi colonize plants growing on agricultural and Mount Salak supporting forest lands in the Villages of Parakan Salak and Cimalati, Sukabumi; and 2) to screen for their biocontrol agent activity againt R. solani. Diameter of R. solani was measured on day 2 after inoculation, and its percent inhibition of growth by endophytic fungi is calculated using the formula Skidmore & Dickinson (1976). The results indicated that 214 isolates of endophytic fungi were isolated from 96 plant species that growing in both places. Endophytic fungi isolated including in the group of Aspergillus, Cladosporium, Colletotrichum, Curvularia, Drechslera, Fusarium, Guignardia, Mucor, Nigrospora, Paecilomyces, Penicillium, Pestalotiopsis, Phoma, Phomopsis, Rhizoctonia, and endophytic fungi that have not been identified. Of the 214 fungal isolates tested, 39 isolates (18.22 %) could inhibit the growth of R. solani from 10.18 % to 58.99 % with a percent inhibition. The highest growth inhibition of R. solani were shown respectively by an unidentified fungus isolatedfrom Hyptis capitata Jack (58.99%), Cladosporium sp. isolated from jeruk bali (Citrus grandis (L.) Osbeck) (55.42%), Pestalotiopsis sp. isolated from pine apple (Ananas comosus (L.) Merr.) (53.85 %), and Paecilomyces lilacinus (Thom) Samson isolated from banyan (Ficus benyamina L.) (51.81%)
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Ababutain, Ibtisam Mohammed, Sahar Khamees Aldosary, Amal Abdulaziz Aljuraifani, Azzah Ibrahim Alghamdi, Amira Hassan Alabdalall, Eida Marshid Al-Khaldi, Sumayh A. Aldakeel, Noor B. Almandil, Sayed AbdulAzeez und J. Francis Borgio. „Identification and Antibacterial Characterization of Endophytic Fungi from Artemisia sieberi“. International Journal of Microbiology 2021 (05.03.2021): 1–11. http://dx.doi.org/10.1155/2021/6651020.
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Endophytic fungi serve as a reservoir for important secondary metabolites. The current study focused on the antibacterial properties of endophytic fungi isolated from Artemisia sieberi. Initially, six endophytic fungi were isolated and purified from the stem of A. sieberi. Endophytic fungi were identified by morphological characteristics, as well as by molecular identification using 18S rRNA gene sequencing method. All the six isolates were subjected to the preliminary screening for their antibacterial activity against nine important pathogenic bacteria using the disk-diffusion method. Crude extracts of the most active isolate were obtained using ethyl acetate. Antibacterial activity of the ethyl acetate extract was evaluated using well diffusion method on the selected isolate. The antibacterial efficiency of the selected isolate was evaluated by determining the Minimum Inhibitory Concentration (MIC). MIC values were in appreciable quantity against both Gram-positive and Gram-negative bacteria ranging from 3.125 to 6.25 µg/mL and 12.5 to 50 µg/mL, respectively. This result indicated that Gram-positive bacteria were more susceptible to the endophytic fungi extract. Moreover, the molecular identification results revealed that all the isolates belong to Ascomycota and represented Aspergillus and Penicillium genera and three species: A. oryzae (three isolates), A. niger (one isolate), and P. chrysogenum (two isolates). All six endophytic fungi were able to inhibit the growth of at least two of the tested bacteria. Among the isolated strains, isolate AS2, which identified as P. chrysogenum, exhibited the highest antibacterial activity against all nine tested bacteria and was higher than or equal to the positive control against most of the tested bacteria. Future studies are required to isolate and identify these bioactive substances, which can be considered as a potential source for the synthesis of new antibacterial drugs to treat infectious diseases.
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Pakshir, Keyvan, Moosa Rahimi Ghiasi, Kamiar Zomorodian und Ali Reza Gharavi. „Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran“. BioMed Research International 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/619576.
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Introduction. Keratinophilic fungi are an important group of fungi that live in soil. The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in Shiraz.Materials and Methods. A total of 196 soil samples from 43 parks were collected. Isolation of the fungi was performed by hair bait technique. The isolated colonies were identified by morphologic feature of macro- and microconidia and molecular method, using DNA sequence analysis. ITS region of ribosomal DNA was amplified and the PCR products were sequenced.Results. 411 isolates from 22 genera were identified.Fusarium(23.8%),Chrysosporium(13.13%),Acremonium(12.65%),Penicillium(12.39%),Microsporum gypseum(1.94%),Bionectria ochroleuca(1.21%),Bipolaris spicifera(1.21%),Scedosporium apiospermum(0.82%),Phialophora reptans(0.82%),Cephalosporium curtipes(0.49%),Scedosporium dehoogii(0.24%),Ochroconis constricta(0.24%),Nectria mauritiicola(0.49%),Chaetomium(0.49%),Scopulariopsis(0.24%),Malbranchea(0.24%), andTritirachium(0.24%) were the most important isolates. Most of the fungi were isolated from the soils with the PH range of 7 to 8.Conclusion. Our study results showed that many keratinophilic fungi isolated from the parks soil are important for public health and children are an important group at a high risk of being exposed to these fungi.
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Biedukiewicz, Anna. „Yeast-like fungi isolated in students“. Acta Mycologica 42, Nr. 1 (23.12.2013): 141–49. http://dx.doi.org/10.5586/am.2007.015.
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The occurrence of yeast-like fungi in the most important infection portals of the respiratory system in 200 randomly chosen students of biology and veterinary medicine was examined. The students come into direct contact with plants and animals that may be colonised by fungi belonging to various systematic groups. Nine species of yeast-like fungi, including 7 species determined in the biologists, were recorded in the subjects. <em>Candida tropicalis</em> and <em>Candida albicans</em> were the most frequently isolated fungi. The greatest number of fungi was isolated from the oral cavity (124 isolates), fewer from the throat (79 isolates), and the smallest number from the nose (8 isolates). Fungi occurred more frequently in autumn and slightly less frequently in spring, and were isolated more frequently from women than from men.
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Lima, Milton Luiz da Paz, Maria Cristina Araújo Vaz, Aline Suelen da Silva, Karoliny De Almeida Souza und Gabriel Isaias Lee Tuñon. „In vitro CONFRONTATION OF Trichoderma spp. ISOLATES WITH PHYTOPATHOGENIC AND NON-PHYTOPATHOGENIC FUNGI“. JOURNAL OF NEOTROPICAL AGRICULTURE 3, Nr. 2 (02.09.2016): 1–8. http://dx.doi.org/10.32404/rean.v3i2.810.
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The aim of this study was the confrontation of Trichoderma spp. with pathogenic and non-pathogenic fungi. The Trichoderma spp. and filamentous fungi (Fusarium solani, Alternaria sp., Colletotrichum gloeosporioides, and Aspergillus niger) were transferred to potato-dextrose-agar (PDA). The confrontation was performed using Petri dishes inoculated with mycelial disks in opposite position (9 mm) of Trichoderma spp. and filamentous fungi. After inoculation the plates remained under 25 ° C regime to 12 hours light for a period of seven days. After this period was evaluated qualitatively according to the scale of Bell et al. (1982), which consists of the suitability scores for the area percentage of the culture medium under expression of antagonism. The isolate of C. gloeosporioides (jatropha) was isolated which had statistically slightly inhibited growth by Trichoderma spp., Unlike the isolated Alternaria sp. (parsley) was isolated micelial fungi suffered the most antagonism. Statistically the Trichoderma isolate derived from pineapple promoted the highest antagonistic activity against isolates of pathogenic fungi tested, being a candidate for use in biocontrol.
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Vaz, Aline B. M., Rubens C. Mota, Maria Rosa Q. Bomfim, Mariana L. A. Vieira, Carlos L. Zani, Carlos A. Rosa und Luiz H. Rosa. „Antimicrobial activity of endophytic fungi associated with Orchidaceae in Brazil“. Canadian Journal of Microbiology 55, Nr. 12 (Dezember 2009): 1381–91. http://dx.doi.org/10.1139/w09-101.
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The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of Orchidaceae collected in a Brazilian tropical ecosystem. In total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude extracts. Thirty-three percent of the isolates displayed antimicrobial activity against at least one target microorganism. The multivariate statistical analyses conducted indicate that the extracts of endophytic fungi isolated from leaves of terrestrial orchids in semideciduous forest were more active against Escherichia coli , whereas extracts of endophytic fungi from roots of rupicolous orchids collected in rock fields were more active against Candida krusei and Candida albicans . Among the fungi that were screened in the study, 22 isolates held their antimicrobial activities after replication and were therefore selected for assessment of the minimum inhibitory concentration (MIC), which ranged from 62.5 to 250 µg/mL and 7.8 to 250 µg/mL against bacteria and fungi, respectively. One isolate of Alternaria sp. and one isolate of Fusarium oxysporum presented the strongest antibacterial activity. Three Fusarium isolates, Epicoccum nigrum , and Sclerostagonospora opuntiae showed the greatest MIC values against the pathogenic yeasts. This study is the first survey investigating the bioactive potential of endophytic fungi associated with tropical Orchidaceae species present in Brazilian ecosystems.
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Gofar, Nuni. „Characterization of Petroleum Hydrocarbon Decomposing Fungi Isolated from Mangrove Rhizosphere“. Journal of Tropical Soils 16, Nr. 1 (01.07.2013): 39–45. http://dx.doi.org/10.5400/jts.2011.v16i1.39-45.
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The research was done to obtain the isolates of soil borne fungi isolated from mangrove rhizosphere which were capable of degrading petroleum hydrocarbon compounds. The soil samples were collected from South Sumatra mangrove forest which was contaminated by petroleum. The isolates obtained were selected based on their ability to survive, to grow and to degrade polycyclic aromatic hydrocarbons in medium containing petroleum residue. There were 3 isolates of soil borne hydrocarbonoclastic fungi which were able to degrade petroleum in vitro. The 3 isolates were identified as Aspergillus fumigates, A. parasiticus, and Chrysonilia sitophila. C. sitophila was the best isolate to decrease total petroleum hydrocarbon (TPH) from medium containing 5-20% petroleum residue.Keywords: Hydrocarbonoclastic fungi, hydrocarbon compounds, mangrove rhizosphere
Dissertationen zum Thema "The isolated fungi"
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Weinstein, Richard Neil. „Ecophysiology of fungi isolated from soil in an Antarctic fellfield ecosystem“. Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624489.
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Heung, Shing-yan, und 向承恩. „Multilocus sequence typing of Candida albicans strains isolated in Hong Kong“. Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44660005.
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April, Trevor Marc. „Hydrocarbon-degrading filamentous fungi isolated from flare pit soils of northern and western Canada“. Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ40024.pdf.
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Ghaderian, Seyed Majid. „The effect of toxic heavy metals upon fungi of the genus Pythium isolated from soil“. Thesis, University of Sheffield, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301558.
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Schierbaum, Anna. „Occurrence, distribution and agroactive metabolite production of endophytic fungi isolated from marine and shoreline plants“. Thesis, University of Portsmouth, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479128.
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Svahn, Stefan. „Analysis of Secondary Metabolites from Aspergillus fumigatus and Penicillium nalgiovense : Antimicrobial Compounds from Filamentous Fungi Isolated from Extreme Environments“. Doctoral thesis, Uppsala universitet, Avdelningen för farmakognosi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-242611.
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This thesis describes the cultivation and extraction of filamentous fungi isolated from extreme environments in the search for new antibiotic compounds. Filamentous fungi are a rich source of medicines including antibiotics, and it is believed that many currently unknown fungal species and bioactive fungal metabolites remain to be discovered. Aspergillus fumigatus and Penicillium nalgiovense strains were isolated from an antibiotic-contaminated riverbed near Hyderabad, India, and soil taken from a penguin’s nest on Paulete Island, Antarctica, respectively. It was anticipated that the extreme conditions within these environments would exert unusual selective pressures on their filamentous fungi, possibly causing the secretion of new bioactive compounds. The cultivation, extraction and analysis of metabolites from the A. fumigatus strain resulted in the isolation of the antimicrobial substance gliotoxin. Subsequent investigations revealed that this strain’s secretion of gliotoxin was increased by as much as 65 % when it was cultivated in the presence of pathogen-associated molecular patterns. These results indicate the existence of a fungal receptor/signaling system for detecting nearby bacteria. The scope for using gliotoxin and the related metabolite bis(methyl)gliotoxin as biomarker metabolites for diagnosing the lethal pulmonary condition invasive aspergillosis was also investigated. Bronchoalveolar lavage fluid from 42 patients with and without possible invasive aspergillosis was extracted and analyzed. The results obtained suggest that gliotoxin and bis(methyl)gliotoxin are not suitable markers for diagnosing invasive aspergillosis. Studies on the P. nalgiovense strain from Antarctica resulted in the isolation of the antifungal agent amphotericin B. The secretion of this compound increased when P. nalgiovense was cultured on a potato-dextrose agar enriched with coconut flakes rather than liquid RPMI 1640 medium. This was the first time amphotericin B was isolated from any organism other than the bacterium Streptomyces nodosus. The results presented in this thesis will be useful in the continuing search for novel bioactive compounds, the diagnosis of fungal infections, and as a source of insight into the interactions between microorganisms. Moreover, they show that even extensively studied fungal genera such as Aspergillus and Penicillium are not completely understood and may produce unexpected or previously unknown bioactive metabolites under appropriate conditions.
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Sobreira, Aline Cavalcante Mesquita. „Study chemical and cytotoxic secondary metabolites isolated endophytic fungi from Aroeira-do-SertÃo (Myracrodruon urundeuva Fr. All.):Pseudofusicoccum stromaticum and Lasiodiplodia theobromae“. Universidade Federal do CearÃ, 2016. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16644.
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Empresa Brasileira de Pesquisa AgropecuÃriaBanco do Nordeste do Brasil
Fungos endofÃticos tÃm sido apontados como uma fonte promissora de substÃncias biologicamente ativas, entre as quais compostos com potencial atividade anticÃncer. Neste contexto, o presente trabalho descreve o primeiro estudo de prospecÃÃo quÃmica e citotoxicidade de dois fungos endofÃticos isolados de Aroeira-do-sertÃo (Myracrodruon urundeuva Fr. All.): Pseudofusicoccum stromaticum (MUB58) e Lasiodiplodia theobromae (MUB65). Os extratos de acetato de etila MUB58 e MUB65 foram obtidos atravÃs de partiÃÃo lÃquido-liquido do caldo destas cepas fÃngicas, as quais foram cultivadas por 21 dias em extrato de BD e malte, respectivamente. Os extratos fÃngicos foram inicialmente analisados por cromatografia lÃquida de ultraeficiÃncia acoplada a espectrometria de massas de alta resoluÃÃo (CLUE-EM) e posteriormente submetidos a cromatografia em Sephadex LH-20, seguida de cromatografia lÃquida de alta eficiÃncia com detector de arranjo de diodo. AnÃlise de CLUE-EM permitiu caracterizar a presenÃa de sete metabÃlitos no extrato MUB-58: O-sulfato de colina, xantofusina, 7-hidroxi-1-isocromanona, Ãcido multicÃlico, djalonensona, 3-Ãcido carboxÃlico-8-metoxicarbonila-1-hidroxi-9-oxo-9H-xantona e ciclo-Phe-Leu-Val-Leu-Leu. AlÃm disso, foram isolados quatro metabÃlitos ciclo-L-Phe-D-Leu1-L-Leu2-L-Leu3-L-lle (FC1), 5-hidroxi-metilfurfural (FC2), rotenolona (FC3) e tefrosina (FC4). O composto FC1 à inÃdito na literatura, enquanto os compostos FC3 e FC4 estÃo sendo relatados pela primeira vez em fungos endofÃticos. Da mesma forma, a anÃlise CLUE-EM do extrato MUB-65 levou à caracterizaÃÃo quÃmica de oito compostos jà relatados na espÃcie L. theobromae: 4-hidroximeleina; meleina; (3R, 5R)- 5-hidroxila-de-O-metil-lasiodiplodina; (3R,5R)-hidroxilasiodiplodina; 2,4,6-trimetiloct-2-enoato,1,2,6,8a- tetrahidro-7-hidroxi-1-8a-dimetil-6-oxo-2-naftalenila; lasiodiplodina; lasiojasmonato A e lasiojasmonatos B ou C. Adicionalmente, dois metabÃlitos foram isolados: lasiodiplodina (LT1) e rel-11-12- (7âR*, 4âR*, 2âR*- tetrahidrofuro[1â,2â] piranil)- lasiodiplodina (LT2). O composto LT2 està sendo relatado pela primeira vez na literatura. As estruturas quÃmicas de todas as substÃncias isoladas foram elucidadas por espectrometrias de RessonÃncia MagnÃtica Nuclear de 1H e 13C, infravermelho e CLUE-EM. Os extratos e seus respectivos metabÃlitos isolados foram submetidos ao ensaio de citotoxicidade para avaliaÃÃo dos seus efeitos antiproliferativos frente a uma linhagem celular de cÃncer colorretal (HCT-116). O extrato de MUB58 apresentou significativa atividade (75%) correspondendo a um IC50 de 10,40 Âg.mL-1, enquanto o extrato de MUB65 foi inativo. Os metabÃlitos isolados LT1 (IC50 11,24 Âg.mL-1), FC3 (IC50 5,59 Âg.mL-1) e FC4 (IC50 0,51 Âg.mL-1) apresentaram atividades na faixa de 11,24 a 0,51 Âg.mL-1.
Endophytic fungi have been identified as a promising source of biologically active substances,including compounds with potential anticancer activity. In this context, this paper describes the first study of chemical prospecting and cytotoxicity two endophytic fungi isolated from Aroeira -do-SertÃo(Myracrodruon urundeuva Fr.ll.):Pseudofusicoccum stromaticum (MUB-58) and Lasiodiplodia theobromae (MUB - 65). The ethylacetate extracts MUB-58 and MUB-65 were obta ined by liquid-liquid partition broth these fungal strains which were cultured for 21 days on BD and malt extract , respectively. The fungal extracts were first analyzed by liquid chromatography of ultraefficiency coupled to spectrometry, high resolution mass (CLUE -MS) and subsequently subjected to chromatography on Sephadex LH - 20, followed by liquid chromatography of high efficiency with diode array detector. CLUE - MS analysis allowed to determine the prese nce of metabolites in seven MUB 58 extract: cholin - O - sulfate, xanthofusin, 7-hidroxy -1-isochromanone, multico licacid, djalonensone, 8 - methoxycarbonyl-1-hidroxy-9-oxo-9H-xanthene-3-carboxylix acidand cyclo-Phe-Leu-Val-Leu-Leu. In addition, were isolated four metabolites:cycloL-Phe-D-Leu1-L-Leu2-L-Leu 3-L-Ile (FC1), 5-hidroxy-methilfurfural (FC2), rotenolone (FC3) and tephrosin (FC4).The compound FC1 is unprecedented in the literature, while FC3 and FC4 compounds are being reported for the first time in en dophytic fungi. Likewise, a CLUE - MS analysis the MUB - 65 extract led to characterization of eight chemical compounds already reported in the species L. theobromae:4-hidroxymelein; Mellein;(3R,5R)-5-hidroxyl-de-O-methyl-lasiodiplodin;(3 R,5 R)- hydroxyla siodiplodin; 2,4,6-trimethyloct-2-enoic acid-1,2,6,8a-tetrahydro-7-hydroxy-1-8a-dimethyl-6-oxo-2- naphtalenyl-ester;lasiodiplodin;lasiojasmonate A and lasiojasmonates B ou C. Additionally, two metabolites w ere isolated: lasiodiplodin(LT1) and rel-11-12-(7'R* 4'R*, 2 'R*-tetrahydrofuro [1', 2'] pyranyl)-lasiodiplodin (LT2). The compound LT2 being first reported in the literature. The chemical structures of all isolated compounds were elucidated by spectrometries Nuclear Magnetic Resonance 1 H and 13C, IR and CLUE-MS. The extracts and their isolated metabolites were subjected to the cytotoxicity assay to evaluate their antiproliferative effects against a colorectal cancer cell line (HCT-116). The MUB-58 extract showed significant activity (75%) corresponding to an IC50of 10.40 μg.mL -1, while the MUB65 extract was inactive. The LT1 isolated metabolites (IC5011.24 μg.mL-1), FC3(IC50 5.59 μg.mL-1) and FC4(IC500.51 μg.mL-1) showed activity in the range of 11.24 to 0.51 μg.mL-1.
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Sarkar, Tanushree. „Studies on resistance of trichosanthes dioica and their induction with chemical inducers against fungal pathogen“. Thesis, University of North Bengal, 2021. http://ir.nbu.ac.in/handle/123456789/4760.
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Hundley, Nicholas James. „Structure Elucidation of Bioactive Compounds Isolated from Endophytes of Alstonia scholaris and Acmena graveolens“. Diss., CLICK HERE for online access, 2005. http://contentdm.lib.byu.edu/ETD/image/etd1013.pdf.
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Souza, Eduardo Lorensi de. „Produção de mudas e crescimento de Eucalyptus grandis Hill ex maiden inoculado com fungos ectomicorrízicos em área arenizada“. Universidade Federal de Santa Maria, 2009. http://repositorio.ufsm.br/handle/1/5492.
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Conselho Nacional de Desenvolvimento Científico e TecnológicoEucalyptus. grandis is one of the main species used in forestry, in the Rio Grande do Sul State. The reforestations have been concentrated in low fertility soil areas, especially in phosphorus, such as the Quartzarenic Neosoils, which occur in the south of the state. It represents a problem for the establishment of this culture in field. This forestry species has the capacity of making symbiosis with ectomycorrhizal fungi that increase plant grown and the absorption of water and nutritious. The aim of the work was to evaluate the initial growth of eucalyptus in sandy area and the ectomycorrhizal fungi inoculation isolated, individually or mixed in Neosoil. In the first stage, eucalyptus seedlings were produced in greenhouse, inoculated or no-inoculated with the UFSC-Pt116 isolated, produced in peat or in Neosoil. After 120 days seedlings were transplanted to an area subjected to the process of sanding in São Francisco de Assis and evaluated regarding the survival, height, stem s diameter and tenors of nitrogen, phosphorus and potassium, total phosphorus, inorganic phosphorus, and wood production. In the second stage it was tested, in greenhouse, the effect of ectomycorrhizal fungi inoculation with the UFSC-Pt116, UFSC-Pt188 and UFSC-SA9 isolates, individually or mixed in peat substrate and in Neosoil. Determinations were accomplished regarding stem s diameter and height, dry matter of the aerial portion and the roots, roots volume, mycorrhizal colonization as well as the level of nitrogen, potassium and total, organic and inorganic phosphorus of the aerial portion of plants. In field, the plants produced in Neosoil and inoculated with the isolated UFSC-Pt116 obtained the highest survival, stem s height and diameter, nitrogen level, as well as wood production in relation to the no-inoculated seedlings. In the second study, the plants that received individual inoculation of the isolated UFSC-Pt116 and UFSC-SA9 and that were produced with peat obtained the highest height, stem s diameter, mycorrhizal colonization and dry matter accumulation. The plants that were produced in Neosoil and the individually inoculated with the isolated UFSC-Pt116, UFSC-Pt118 and UFSCSA9 showed highest height, stem s diameter, dry matter of the aerial portion and roots volume. The seedlings produced in Neosoil obtained higher height and diameter than those produced in peat.
No Estado do Rio Grande do Sul o E. grandis é uma das principais espécies utilizadas na silvicultura. Os reflorestamentos têm se concentrado em regiões de solos com baixa fertilidade, especialmente em fósforo, como os Neossolos Quartzarênicos, que ocorrem na metade sul do Estado, tornando-se um problema para o estabelecimento dessa cultura no campo. Esta espécie florestal tem a capacidade de formar simbiose com fungos ectomicorrízicos que auxiliam o crescimento das plantas através do aumento na absorção de nutrientes e água. No presente trabalho teve-se por objetivos avaliar o crescimento inicial do eucalipto em área arenizada e a inoculação dos isolados fúngicos ectomicorrízicos, individualmente ou em mistura em Neossolo. Na primeira etapa do trabalho, foram produzidas mudas de eucalipto em casa de vegetação, que foram inoculadas ou não com o isolado UFSC-Pt116, produzidas em turfa ou em Neossolo. Após 120 dias, essas foram transplantadas para uma área arenizada em São Francisco de Assis e avaliadas quanto à sobrevivência, altura, diâmetro do caule e teores de nitrogênio, fósforo e potássio, fósforo total, fósforo inorgânico, fósforo orgânico e produção de madeira. Na segunda etapa testou-se em casa de vegetação o efeito da inoculação dos isolados de fungos ectomicorrízicos UFSC-Pt116, UFSC-Pt188 e UFSC-SA9, individualmente e em mistura no substrato turfa e no Neossolo. Foram realizadas avaliações quanto à altura e diâmetro do caule, massa seca da parte aérea e das raízes, volume de raízes, percentual de colonização micorrízica e teores de nitrogênio, fósforo e potássio da parte aérea das plantas. No campo, as plantas que foram produzidas no Neossolo e inoculadas com o isolado UFSC-Pt116 obtiveram a maior sobrevivência, altura e diâmetro do caule, teores de nitrogênio, bem como produção de madeira em relação às mudas não inoculadas. No segundo estudo, as plantas que receberam inoculação individual dos isolados UFSC-Pt116 e UFSC-SA9 e foram produzidas com turfa obtiveram a maior altura, diâmetro do caule, colonização micorrízica e acúmulo de massa seca. As plantas que foram produzidas em Neossolo e inoculadas com os isolados UFSC-Pt116, UFSC-Pt188 e UFSC-SA9 individualmente alcançaram a maior altura, diâmetro do caule, massa seca da parte aérea e volume de raízes. As mudas produzidas no Neossolo alcançaram altura e diâmetro maiores que as produzidas na turfa.
Bücher zum Thema "The isolated fungi"
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Lawrence, Michelle Irene. A study of anaerobic fungi isolated from ruminants and monogastric herbivores. Manchester: University of Manchester, 1993.
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Restrepo, Angela, Angel A. Gónzalez und Beatriz L. Gómez. Endemic dimorphic fungi. Herausgegeben von Christopher C. Kibbler, Richard Barton, Neil A. R. Gow, Susan Howell, Donna M. MacCallum und Rohini J. Manuel. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780198755388.003.0016.
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Endemic dimorphic infections are acquired by inhalation of fungal spores which undergo a thermal transition to a yeast-like phase in the host. The causative organisms are geographically restricted and are isolated from the environment; likewise, the infections are associated with people living in, or visiting, these endemic regions. The clinical presentations range from asymptomatic to chronic, and disseminated, depending on the host immune status and other risk factors. The infections and their causative agents are: histoplasmosis (Histoplasma capsulatum), paracoccidioidomycosis (Paracoccidioides brasiliensis/lutzii), blastomycosis (Blastomyces dermatitidis/gilchristii), coccidioidomycosis (Coccidioides immitis/posadasii), talaromycosis (previously penicilliosis; Talaromyces [Penicillium] marneffei), and emmonsiosis (Emmonsia species). Diagnosis relies on microscopy and culture, histology, and immunological detection. Owing to their infectious nature, all of these organisms must be handled using biosafety level-3 containment. Treatment is based around azole administration, usually itraconazole, with amphotericin B for the more severe forms or for the most at risk patients.
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Falconi, Cesar E. Epiphytic yeasts isolated from apple leaves to control of gray and blue mold fruit rots of apple. 1996.
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Kosmidis, Chris, David W. Denning und Eavan G. Muldoon. Fungal disease in cystic fibrosis and chronic respiratory disorders. Herausgegeben von Christopher C. Kibbler, Richard Barton, Neil A. R. Gow, Susan Howell, Donna M. MacCallum und Rohini J. Manuel. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780198755388.003.0037.
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A range of fungal disease syndromes affect patients with chronic respiratory diseases and cystic fibrosis (CF). Invasive aspergillosis is increasingly recognized in seriously ill patients with chronic obstructive pulmonary disease, especially after high-dose steroids. Chronic pulmonary aspergillosis affects patients with pre-existing cavities or bullae, such as those with previous tuberculosis or atypical mycobacterial disease, bullous emphysema, sarcoidosis, pneumothorax, or treated lung cancer. In addition, fungi have become one of the most important trigger agents for asthma, and allergic bronchopulmonary aspergillosis may complicate up to 3.5% of cases of asthma and up to 15% of cases of CF, starting in childhood. CF patients are commonly colonized with fungal organisms, although the impact of such colonization on outcome is not clear. Aspergillus is the most common mould isolated from CF patients. Distinguishing between colonization and infection remains challenging. Candida is thought to be of no clinical significance; however, it has been associated with decline in lung function.
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Ianson, David C. Variation in plant response to inoculation with different isolates of vesicular arbuscular mycorrhizal fungi. 1990.
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Pengembangan potensi isolat fungi Glomalean dari areal bekas penambangan emas sebagai agen fitoremediasi: Laporan penelitian hibah bersaing tahun anggaran 2007. [Pontianak]: Universitas Tanjungpura, 2007.
Den vollen Inhalt der Quelle findenBuchteile zum Thema "The isolated fungi"
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Risoli, Samuele, Sabrina Sarrocco, Giuliana Terracciano, Riccardo Baroncelli, Marco Alberto Luca Zuffi, Cecilia Mancusi und Cristina Nali. „Isolation and molecular characterization of Fusarium species (Fungi, Ascomycota) from unhatched eggs of Caretta caretta in Tuscany (Italy)“. In Ninth International Symposium “Monitoring of Mediterranean Coastal Areas: Problems and Measurement Techniques”, 747–55. Florence: Firenze University Press, 2022. http://dx.doi.org/10.36253/979-12-215-0030-1.71.
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Caretta caretta is the only sea turtle species nesting along the Tuscan coastline, where nests are becoming more numerous and widespread, as well as the number of Sea Turtle Egg Fusariosis (STEF)-affected eggs. In this work, we analyzed eggs from natural nests of C. caretta that showed visual symptoms of STEF from Tuscan nests, with the aim to (i) isolate Fusarium spp. strains present in unhatched eggs, (ii) morphologically and molecularly characterize the isolated fungi and, finally, (iii) reconstruct the phylogenetic relationships between our isolates and those already known.
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Bhatt, Pankaj, und Shaohua Chen. „Optimization of Mycoremediation Process for the Isolated Fungi“. In Springer Protocols Handbooks, 101–7. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2006-9_9.
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Luo, Zhu-Hua, Ka-Lai Pang, Yi-Rui Wu, Ji-Dong Gu, Raymond K. K. Chow und L. L. P. Vrijmoed. „Degradation of Phthalate Esters by Fusarium sp. DMT-5-3 and Trichosporon sp. DMI-5-1 Isolated from Mangrove Sediments“. In Biology of Marine Fungi, 299–328. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-23342-5_15.
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Florio Furno, Matteo, Davide Ferrero, Anna Poli, Valeria Prigione, Maria Tuohy, Matteo Oliva, Carlo Pretti und Giovanna Cristina Varese. „Fungi from the sediments of the harbour of Livorno as potential bioremediation agents“. In Ninth International Symposium “Monitoring of Mediterranean Coastal Areas: Problems and Measurement Techniques”, 667–76. Florence: Firenze University Press, 2022. http://dx.doi.org/10.36253/979-12-215-0030-1.63.
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The harbour's sediments are among the biotypes most affected by contamination by pollutants due to anthropogenic activities. The porpoise of this work is to perform a preliminary screening on 74 fungi previously isolated in the polluted sediments of the harbour of Livorno, to identify those endowed with oxidative capabilities and to evaluate the potential producers of metabolites or enzymes of interest, for potential applications in future environmental bioremediation. The results have shown that 26 (35.1%) out of 74 tested fungi produced positive oxidation signal on at least one media
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Gibrat, R., H. Barbier-Brygoo, J. Guern und C. Grignon. „Transtonoplast Potential Difference and Surface Potential of Isolated Vacuoles“. In Biochemistry and Function of Vacuolar Adenosine-Triphosphatase in Fungi and Plants, 83–97. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-70320-1_8.
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Buchalo, Asya S., Solomon P. Wasser, Hans P. Molitoris, Paul A. Volz, Irina Kurchenko, Ingeborg Lauer und Bhavin Rawal. „Species Diversity and Biology of Fungi Isolated from the Dead Sea“. In Evolutionary Theory and Processes: Modern Perspectives, 283–300. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4830-6_17.
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Korneykova, M. V., A. A. Chaporgina und V. V. Redkina. „Oil Destructive Activity of Fungi Isolated from the Soils of the Kola Peninsula“. In Springer Geography, 123–34. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-89602-1_16.
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AlBany, Yousif A., Anwer N. Mamdoh und Mohammad I. Al-Berfkani. „Hydrocarbon Degradation of Oil Pipeline Blockage by Thermophilic Fungi Isolated from Tawke Field“. In Springer Proceedings in Earth and Environmental Sciences, 147–58. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-18641-8_11.
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Ekanayake, Manavi S., Dhanushka Udayanga und Pathmalal M. Manage. „Mycoremediation of Synthetic Textile Dyes by Fungi Isolated from Textile Wastewater Effluent and Soil“. In Springer Protocols Handbooks, 31–43. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2006-9_4.
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Talukdar, Rajreepa, Sudem Wary, Rashmeeta Hajowary, Anindita Sarma und Kumanand Tayung. „Antimicrobial Activity of Endophytic Fungi Isolated from Some Selected Ethnomedicinal Plants of Assam, India“. In Endophytes, 91–102. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-9371-0_6.
Der volle Inhalt der QuelleKonferenzberichte zum Thema "The isolated fungi"
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Щербакова, Татьяна, Штефан Кручан, Борис Пынзару und Леонид Волощук. „Антагонизм новых изолятов грибов рода Trichoderma по отношению к патогену Alternaria Sp., выделенному из ореха грецкого“. In International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.39.
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The research is aimed at studying the antagonistic relationships of five isolates of the genus Trichoderma fungi in relation to the Alternaria sp. fungus. The pathogen was isolated from diseased walnut leaves. In the work was used the method of double cultures on agar media. As a result, 160 the inhibition rate of Alternaria pathogen by Trichoderma fungi was determined. For isolate №3 the rate was 90%, for isolate №2 - 85%, isolate №4 inhibited the pathogen by 75%, isolate №1 - by 65%. Isolate №5 and strains T.virens and T. lignorum suppressed the growth of Alternaria by 40-50%.
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Widayanti, Gusti Ayu, Hary Widjajanti, Salni Salni und Agus Geter Edy Sutjipto. „Identification of Endophytic Fungi on the Leaves of Jeruju (<i>Acanthus ilicifolius</i>) which are Potential as Antibacteria“. In International Conference on Industrial Sciences, Engineering and Technology toward Digital Era 2023. Switzerland: Trans Tech Publications Ltd, 2024. http://dx.doi.org/10.4028/p-lzq74e.
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Endophytic fungi are microbes that live in plant tissues and can synthesize the same active biochemical compounds as their hosts. This study aims to determine the type of endophytic fungi of Jeruju leaves (Acanthus ilicifolius) isolate DJS1. This research was conducted in January 2020 and is a type of descriptive qualitative research. The subjects in this study were one type of endophytic fungi isolate that has the potential as an antibacterial. Identification of endophytic fungi of Jeruju leaves (Acanthus ilicifolius) isolated DJS1 which have antibacterial potential by observing macroscopic and microscopic morphological phenotypic characters. Macroscopic observations described the form of colony structure, aerial hyphae forms, and radial lines that appeared on the fungal isolates. Microscopic observations were made to observe the shape of the hyphae and the shape of the conidia. While molecular observations were carried out by amplifying DNA using ITS primers.
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Sharma, Vinay, Rachana Jain und Jyoti Saxena. „Phosphate solubilization by fungi isolated from alkaline soils“. In MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0033.
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Happitiya, H. A. D. N. N., C. M. Nanayakkara, K. G. S. U. Ariyawansa, S. S. Ediriweera, N. N. Wijayawardene, R. P. P. K. Jayasinghe, Don Qin Dai und S. C. Karunarathna. „Antibacterial Activities of Lichen-associated Fungi in Mangrove Ecosystems in Sri Lanka as Potent Candidates for Novel Antibiotic Agents“. In SLIIT International Conference on Advancements in Sciences and Humanities 2023. Faculty of Humanities and Sciences, SLIIT, 2023. http://dx.doi.org/10.54389/slzp7371.
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Antimicrobial resistance is a global threat to humans, prompting an increasing interest in exploring and developing novel antimicrobial substances derived from diverse sources. Together with the emergence of new diseases the search for novel drug leads has intensified. Less explored microbial habitats have become prime targets in mining for novel antimicrobial molecules. Secondary metabolites synthesized by lichen-associated fungi are good potential targets in this regard. Hence, this study was carried out to explore the antibacterial potential of lichen associated fungi in mangrove ecosystems by taking National Aquatic Resources Research and Development Agency (NARA) Regional Research Centre, Kalpitiya, Puttalam District, Sri Lanka as the study site. Lichen-associated fungi were isolated from collected lichens by plating out surface sterilized lichen thalli pieces. Antibacterial activities of the isolates were tested using two gram-positive bacteria: Staphylococcus aureus and Bacillus cereus and two gram-negative bacteria: Pseudomonas aeruginosa and Escherichia coli. In this study, 72 putative fungal isolates were primarily screened for their antibacterial activity using agar plug diffusion assay and ethyl acetate crude fungal extracts of nine fungal isolates with marked activity were secondarily screened using the well diffusion assay in triplicate. Isolate LIF 0803 identified as Trichosporon faecale showed the most outstanding antibacterial activities as 2.58 ± 0.29, 3.43 ± 0.05, 4.2 ± 0, 4.5 ± 0.14 cm of zone diameter at 100 mg/mL and 1.95 ± 0.59, 3.08 ± 0.13, 3.7 ± 0.12, 4.3 ± 0.19 cm of zone diameter at 50 mg/mL against P. aeruginosa, S. aureus, B. cereus, and E. coli. All nine fungal isolates showed promising antimicrobial activity against both gram positive and negative bacteria. Therefore, this study showed that lichen-associated fungi in mangrove ecosystems have potent antibacterial activities. Hence, bioassay guided fractionation of active compounds from lichen-associated fungi and structure elucidation are warranted.
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J. AL-JASSANI, Mohammad, Huda Hazim AL-TAEE und Asmaa Mansour AL-HAKEEM. „MOLECULAR IDENTIFICATION OF SOME ORNAMENTAL PLANT ROOT ROTTING FUNGI“. In VII. INTERNATIONAL SCIENTIFIC CONGRESSOF PURE,APPLIEDANDTECHNOLOGICAL SCIENCES. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress7-17.
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Plants produced for their beauty rather than their practical utility are known as ornamental plants. There are several sorts of attractive plants such as Rubber, Gladiolus, Iris, and others. Fungi impacting these plants were isolated and identified using morphological and molecular approaches, including PCR and sequencing the ITS region for the most prominent isolates. The isolation results showed the presence of Fusarium ptoliferatum from Rubber, Paecilomyces tenuis from Gladiolus and Rhizoctonia solani from Iris. Sequencing revealed new Fusarium ptoliferatum, Paecilomyces tenuis and Rhizoctonia solani isolates that have been registered at the GeneBank database and registered under the accession number MT935588, MT936328, and MT946901 respectively.
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Alasmar, Reem Moath, und Samir Jaoua. „Investigation and Biological Control of Toxigenic Fungi and Mycotoxins in Dairy Cattle Feeds“. In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0065.
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Mycotoxins, the secondary fungal metabolites are important contaminants of food and feed. Among the other contaminants, aflatoxin B1 (AFB1) and OTA are frequently detected in the animal feed product. In the present study, the mixed dairy cow feed products were collected from the supermarkets in Qatar and analyzed for the presence of AFB1 and OTA. Yeast strains were isolated and tested for their biological control activities against aflatoxigenic and ochratoxin fungi. We demonstrated that local 15 yeasts isolates have important antifungal potential activities through the synthesis of volatile organic compounds (VOC) that are able to act against the mycotoxigenic fungi and their synthesis of the mycotoxins. Two Yeast strains (4&2) isolated from fermented food, have shown a great antifungal inhibition growth in-vitro as well as spores inhibition and mycotoxins synthesis.
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Mahdi JABIR, Dhuha, Saba abdulameer Kadhim AL- ZIADI und Ahmed Abdulameer KADHIM. „INHIBITORY EFFECT OF SOME COMMERCIAL DETERGENTS ON FUNGI ISOLATED FROM INDOOR AIR“. In IV.International Scientific Congress of Pure,Appliedand Technological Sciences. Rimar Academy, 2022. http://dx.doi.org/10.47832/minarcongress4-27.
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The present study was aimed to investigate about the inhibitory effect of some detergents spread in the Iraqi markets contain a group of fungi that pollute the air of homes for this purpose a group of indoor air pollutant fungi of the following species have been isolated such as(Aspergillus niger, Alternaria alternata, Fusarium solani, F.oxysporum, Mucore sp., Rhizopus stolanifer and Penicillium) .The species (Penicillium, Fusarium solani, and Aspergillus niger) isolated from six houses chosen randomly in variant residential neighborhoods were selected to test the ability of some commercial deteragent handwashing liquid Ays trade mark, white bleach(sodium hypochlorate) and Al Emlaq super jel(multi uses cleaning jell), which were randomly tested from the local markets of Diwaniyah city in inhibiting their growth, the results showed that Ays brand hand wash did not affect the fungus Penicillium at any concentration of the concentrations used in the study, while it had an inhibitory effect on the other two air pollutant fungi (Fusarium solani, and Aspergillus niger) while hypochlorite sodium and Al Emlaq super jel were effective on all fungi used in the study
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Hewagama, H. L., G. M. T. K. Somarathna, L. Herath und S. E. Peiris. „Living Colours: Development of Microbial Culture Collection for Use as Microbial Colour Pigments in Textile Dyes“. In SLIIT International Conference on Advancements in Sciences and Humanities 2023. Faculty of Humanities and Sciences, SLIIT, 2023. http://dx.doi.org/10.54389/ccoj7801.
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The textile industry is one of the largest worldwide polluters of clean water due to the heavy use of synthetic dyes. Synthetic dyes are harmful to aquatic life and to human health. To overcome this, natural dyes are being explored as a healthier and more eco-friendly alternative. Several advantages such as ease of extraction, availability, high yields and no seasonal variation make microbial pigments the most ideal source of natural pigments. This study was done to isolate colour pigment producing bacteria and fungi from soil collected from organic farms from various locations in Sri Lanka. In total, 9 pigment producing bacteria and 3 pigment producing fungi were isolated. Gause’s synthetic agar yielded the most pigmented isolates. Extracellular pigments produced by 5 of the bacterial isolates were extracted by a water based method. The antibacterial activity of the pigments in their crude and concentrated forms was tested using the well diffusion method against E.coli ATCC 8739 and Staphylococcus aureus ATCC 6538P. Inhibition zone against S.aureus was observed for both crude (12.33±0.58mm) and concentrated pigments (9.67±0.58mm) extracted from purple pigment producing bacterial isolate (BPU). This pigment has the potential to be used in antibacterial textile preparation. Extracted pigments were used to dye scoured cotton fabric with the use of 3% alum as mordant. Pigment from BPU isolate resulted in better coloured fabric.
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Nuryanti, Siska, Herwin, Fitriana, Sugiyatri Sultan und Risqianty Nasir. „Antibacterial activity of endophytic fungi isolated from Portulaca Oleracea L“. In IWOSP 2021, INTERNATIONAL WORKSHOP ON STATISTICAL PHYSICS. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0124016.
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VASILIAUSKIENĖ, Dovilė, Giedrius BALČIŪNAS und Jaunius URBONAVIČIUS. „ISOLATION AND IDENTIFICATION OF FUNGI GROWING ON FIBRE HEMP SHIVE BASED THERMAL INSULATION MATERIALS“. In Conference for Junior Researchers „Science – Future of Lithuania“. VGTU Technika, 2018. http://dx.doi.org/10.3846/aainz.2018.007.
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Green thermal insulation materials are ecological materials with hemp, linen, jute, wood waste, maize starch, and other types of waste added to polymer-based composites. Such kind of materials are susceptible to the microbial action which can lead to changes of physico-chemical properties of materials, their destruction and also health problems in humans. Here, we isolated and identified several fungal strains that grow on fibre hemp shive based materials. Three isolates were identified as belonging to Trichoderma (Hypocrea) genus. Our further experiments will be concentrated on the monitoring of growth of newly isolated fungi on and/or inside the materials of interest, isolation and characterisation of the hydrolytic enzymes as well as following the mode of material destruction caused by the fungal growth.
Berichte der Organisationen zum Thema "The isolated fungi"
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Lovera, Andrea, Mariano Belaich, Cindy Mejía, Laura Villamizar, Manuel A. Patarroyo, und Gloria Barrera. Characterization of chitinases of Beauveria bassiana (Bv ) induced in semisolid-state fermentation. Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA, 2012. http://dx.doi.org/10.21930/agrosavia.poster.2012.11.
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A major consideration in the development of biopesticides based on entomopathogenic fungi has been the virulence and pathogenicity in terms of mean time of mortality and mean lethal concentration against the target insect. Virulence factors as chitinases degrade insect chitin facilitating the hyphal penetration through the cuticle. In this sense, Beauveria bassiana chitinases are an important cuticle-degrading group of enzymes implicated in the pathogenesis to insects, with high potential to develop biotechnological tools for in pest control. The aim of this study was to characterize chitinases produced by a Colombian strain of B. bassiana (Bv062) isolated from a Diatraea sp. larvae naturally infected in a sugar cane crop.
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Rodriguez, Russell, und Stanley Freeman. Characterization of fungal symbiotic lifestyle expression in Colletotrichum and generating non-pathogenic mutants that confer disease resistance, drought tolerance, and growth enhancement to plant hosts. United States Department of Agriculture, Februar 2005. http://dx.doi.org/10.32747/2005.7587215.bard.
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Fungal plant pathogens are responsible for extensive annual crop and revenue losses throughout the world. To better understand why fungi cause diseases, we performed gene-disruption mutagenesis on several pathogenic Colletotrichum species and demonstrated that pathogenic isolates can be converted to symbionts (mutualism, commensalism, parasitism) expressing non-pathogenic lifestyles. The objectives of this proposal were to: 1- generate crop-specific mutants by gene disruption that express mutualistic lifestyles, 2- assess the ability of the mutualists to confer disease resistance, drought tolerance, and growth enhancement to host plants, 3- compare fslm1 sequences and their genomic locations in the different species, and 4- document the colonization process of each Colletotrichum species.It was demonstrated that wildtype pathogenic Colletotrichum isolates, can be converted by mutation from expressing a pathogenic lifestyle to symbionts expressing non-pathogenic lifestyles. In the US, mutants of Colletotrichum were isolated by homologous gene disruption using a vector containing a disrupted FSlm1 sequence while in Israel, C. acutatum mutants were selected by restriction enzyme mediated integration (REMI) transformation. One group (US) of non-pathogenic mutants conferred disease protection against pathogenic species of Colletotrichum, Fusarium, and Phytophthora; drought tolerance; and growth enhancement to host plants. These mutants were defined as mutualists and disease resistance correlated to a decrease in the time required for hosts to activate defense systems when exposed to virulent fungi. The second group (Israel) of non-pathogenic mutants did not confer disease resistance and were classified as commensals. In addition, we demonstrated that wildtype pathogenic Colletotrichum species can express non-pathogenic lifestyles, including mutualism, on plants they colonize asymptomatically. The expected long term contribution of this research to agriculture in the US and Israel is threefold. Host-specific mutualists will be utilized in the various crops to confer (1) disease resistance to reduce dependence on chemical fungicides; (2) drought tolerance to reduce water consumption for irrigation; (3) growth enhancement to increase yields.
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Harman, Gary E., und Ilan Chet. Enhancement of plant disease resistance and productivity through use of root symbiotic fungi. United States Department of Agriculture, Juli 2008. http://dx.doi.org/10.32747/2008.7695588.bard.
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The objectives of the project were to (a) compare effects ofT22 and T-203 on growth promotion and induced resistance of maize inbred line Mol7; (b) follow induced resistance of pathogenesis-related proteins through changes in gene expression with a root and foliar pathogen in the presence or absence of T22 or T-203 and (c) to follow changes in the proteome of Mol? over time in roots and leaves in the presence or absence of T22 or T-203. The research built changes in our concepts regarding the effects of Trichoderma on plants; we hypothesized that there would be major changes in the physiology of plants and these would be reflected in changes in the plant proteome as a consequence of root infection by Trichoderma spp. Further, Trichoderma spp. differ in their effects on plants and these changes are largely a consequence of the production of different elicitors of elicitor mixtures that are produced in the zone of communication that is established by root infection by Trichoderma spp. In this work, we demonstrated that both T22 and T-203 increase growth and induce resistance to pathogens in maize. In Israel, it was shown that a hydrophobin is critical for root colonization by Trichoderma strains, and that peptaibols and an expansin-like protein from Ttrichoderma probably act as elicitors of induced resistance in plants. Further, this fungus induces the jasmonate/ethylene pathway of disease resistance and a specific cucumber MAPK is required for transduction of the resistance signal. This is the first such gene known to be induced by fungal systems. In the USA, extensive proteomic analyses of maize demonstrated a number of proteins are differentially regulated by T. harzianum strain T22. The pattern of up-regulation strongly supports the contention that this fungus induces increases in plant disease resistance, respiratory rates and photosynthesis. These are all very consistent with the observations of effects of the fungus on plants in the greenhouse and field. In addition, the chitinolytic complex of maize was examined. The numbers of maize genes encoding these enzymes was increased about 3-fold and their locations on maize chromosomes determined by sequence identification in specific BAC libraries on the web. One of the chitinolytic enzymes was determined to be a heterodimer between a specific exochitinase and different endochitinases dependent upon tissue differences (shoot or root) and the presence or absence of T. harzianum. These heterodimers, which were discovered in this work, are very strongly antifungal, especially the one from shoots in the presence of the biocontrol fungus. Finally, RNA was isolated from plants at Cornell and sent to Israel for transcriptome assessment using Affymetrix chips (the chips became available for maize at the end of the project). The data was sent back to Cornell for bioinformatic analyses and found, in large sense, to be consistent with the proteomic data. The final assessment of this data is just now possible since the full annotation of the sequences in the maize Affy chips is just now available. This work is already being used to discover more effective strains of Trichoderma. It also is expected to elucidate how we may be able to manipulate and breed plants for greater disease resistance, enhanced growth and yield and similar goals. This will be possible since the changes in gene and protein expression that lead to better plant performance can be elucidated by following changes induced by Trichoderma strains. The work was in, some parts, collaborative but in others, most specifically transcriptome analyses, fully synergistic.
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Freeman, Stanley, Russell Rodriguez, Adel Al-Abed, Roni Cohen, David Ezra und Regina Redman. Use of fungal endophytes to increase cucurbit plant performance by conferring abiotic and biotic stress tolerance. United States Department of Agriculture, Januar 2014. http://dx.doi.org/10.32747/2014.7613893.bard.
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Major threats to agricultural sustainability in the 21st century are drought, increasing temperatures, soil salinity and soilborne pathogens, all of which are being exacerbated by climate change and pesticide abolition and are burning issues related to agriculture in the Middle East. We have found that Class 2 fungal endophytes adapt native plants to environmental stresses (drought, heat and salt) in a habitat-specific manner, and that these endophytes can confer stress tolerance to genetically distant monocot and eudicot hosts. In the past, we generated a uv non-pathogenic endophytic mutant of Colletotrichum magna (path-1) that colonized cucurbits, induced drought tolerance and enhanced growth, and protected 85% - 100% against disease caused by certain pathogenic fungi. We propose: 1) utilizing path-1 and additional endophtyic microorganisms to be isolated from stress-tolerant local, wild cucurbit watermelon, Citrulluscolocynthis, growing in the Dead Sea and Arava desert areas, 2) generate abiotic and biotic tolerant melon crop plants, colonized by the isolated endophytes, to increase crop yields under extreme environmental conditions such as salinity, heat and drought stress, 3) manage soilborne fungal pathogens affecting curubit crop species growing in the desert areas. This is a unique and novel "systems" approach that has the potential to utilize natural plant adaptation for agricultural development. We envisage that endophyte-colonized melons will eventually be used to overcome damages caused by soilborne diseases and also for cultivation of this crop, under stress conditions, utilizing treated waste water, thus dealing with the limited resource of fresh water.
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Dickman, Martin B., und Oded Yarden. Role of Phosphorylation in Fungal Spore Germination. United States Department of Agriculture, August 1993. http://dx.doi.org/10.32747/1993.7568761.bard.
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Spore germination is a common and fundamental event in fungal development and in many instances an essential phase of fungal infection and dissemination. Spore germination is also critical for hyperparasites to function as biocontrol agents as well as in fermentation proceses. Our common objective is to understand the mechanisms which regulated spore germination and identify factors involved in pathogenicity related prepenetration development. Our approach is to exploit the overall similarity among filamentous fungi using both a plant pathogen (Colletotricum trifolii) and a model system that is genetically sophisticated (Neurospora crassa). The simulataneous use of two organisms has the advantage of the available tools in Neurospora to rapidly advance the functional analysis of genes involved in spore germination and development of an economically important fungal phytopathogen. Towards this we have isolated a protein kinase gene from C. trifolii (TB3) that is maximally expressed during the first hour of conidial germination and prior to any visible gene tube formation. Based on sequence similarities with other organisms, this gene is likely to be involved in the proliferative response in the fungus. In addition, TB3 was able to functionally complement a N. crassa mutant (COT-1). Pharmacological studies indicated the importance of calmodulin in both germination and appressorium differentiation. Using an antisense vector from N. crassa, direct inhibition of calmodulin results in prevention of differentiation as well as pathogenicity. Both cAMP dependent protein kinase (PKA) and protein kinase C (PKC) like genes have been cloned from C. trifolii. Biochemical inhibition of PKA prevents germination; biochemical inhibitors of PKC prevents appressorium differentiation. In order to analyze reversible phosphorylation as a regulatory mechanism, some ser.thr dephosphorylative events have also been analyzed. Type 2A and Type 2B (calcineurin) phosphatases have been identified and structurally and functionally analyzed in N. crassa during this project. Both phosphatases are essential for hyphal growth and maintenance of proper hyphal architecture. In addition, a first novel-type (PPT/PP5-like) ser/thr phosphatase has been identified in a filamentous fungus. The highly collaborative project has improved our understanding of a fundamental process in fungi, and has identified targets which can be used to develop new approaches for control of fungal plant pathogens as well as improve the performance of beneficial fungi in the field and in industry. In addition, the feasibility of molecular technology transfer in comparative mycology has been demonstrated.
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Thomashow, Linda, Leonid Chernin, Ilan Chet, David M. Weller und Dmitri Mavrodi. Genetically Engineered Microbial Agents for Biocontrol of Plant Fungal Diseases. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696521.bard.
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The objectives of the project were: a) to construct the site-specific integrative expression cassettes carrying: (i) the chiA gene for a 58-kDa endochitinase, (ii) the pyrrolnitrin biosynthesis operon, and (iii) the acdS gene encoding ACC deaminase; b) to employ these constructs to engineer stable recombinant strains with an expanded repertoire of beneficial activities; c) to evaluate the rhizosphere competence and antifungal activity of the WT and modified strains against pathogenic fungi under laboratory and greenhouse conditions; and d) to monitor the persistence and impact of the introduced strains on culturable and nonculturable rhizosphere microbial populations in the greenhouse and the field. The research generally support our concepts that combining strategically selected genes conferring diverse modes of action against plant pathogens into one organism can improve the efficacy of biological control agents. We hypothesized that biocontrol agents (BCAs) engineered to expand their repertoire of beneficial activities will more effectively control soilborne plant pathogens. In this work, we demonstrated that biocontrol activity of Pseudomonas fluorescens Q8r1-96 and Q2-87, both producing the antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) effective against the plant pathogenic fungus Rhizoctonia solani, can be improved significantly by introducing and expressing either the 1.6-kb gene chiA, encoding the 58-kDa endochitinase ChiA from the rhizosphere strain SerratiaplymuthicaIC1270, or the 5.8-kb prnABCDoperon encoding the broad-range antibiotic pyrrolnitrin (Prn) from another rhizosphere strain, P. fluorescens Pf-5. The PₜₐcchiAandPₜₐcprnABCDcassettes were cloned into the integrative pBK-miniTn7-ΩGm plasmid, and inserted into the genomic DNA of the recipient bacteria. Recombinant derivatives of strains Q8r1-96 and Q2-87 expressing the PₜₐcchiA or PₜₐcprnABCD cassettes produced endochitinase ChiA, or Prn, respectively, in addition to 2,4-DAPG, and the recombinants gave significantly better biocontrol of R. solani on beans under greenhouse conditions. The disease reduction index increased in comparison to the parental strains Q8r1-96 and Q2-87 to 17.5 and 39.0% from 3.2 and 12.4%, respectively, in the case of derivatives carrying the PₜₐcchiAcassette and to 63.1 and 70% vs. 2.8 and 12,4%, respectively, in the case of derivatives carrying the PₜₐcprnABCDcassette. The genetically modified strains exhibited persistence and non-target effects comparable to those of the parental strains in greenhouse soil. Three integrative cassettes carrying the acdS gene encoding ACC deaminase cloned under the control of different promoters were constructed and tested for enhancement of plant growth promotion by biocontrol strains of P. fluorescens and S. plymuthica. The integrative cassettes constructed in this work are already being used as a simple and efficient tool to improve biocontrol activity of various PGPR bacteria against fungi containing chitin in the cell walls or highly sensitive to Prn. Some parts of the work (e. g., construction of integrative cassettes) was collaborative while other parts e.g., (enzyme and antibiotic activity analyses) were fully synergistic. The US partners isolated and provided to the Israeli collaborators the original biocontrol strains P. fluorescens strains Q8r1-96 and Q2-87 and their mutants deficient in 2,4-DAPG production, which were used to evaluate the relative importance of introduction of Prn, chitinase or ACC deaminase genes for improvement of the biocontrol activity of the parental strains. The recombinant strains obtained at HUJI were supplied to the US collaborators for further analysis.
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Schwartz, Bertha, Vaclav Vetvicka, Ofer Danai und Yitzhak Hadar. Increasing the value of mushrooms as functional foods: induction of alpha and beta glucan content via novel cultivation methods. United States Department of Agriculture, Januar 2015. http://dx.doi.org/10.32747/2015.7600033.bard.
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During the granting period, we performed the following projects: Firstly, we differentially measured glucan content in several pleurotus mushroom strains. Mushroom polysaccharides are edible polymers that have numerous reported biological functions; the most common effects are attributed to β-glucans. In recent years, it became apparent that the less abundant α-glucans also possess potent effects in various health conditions. In our first study, we explored several Pleurotus species for their total, β and α-glucan content. Pleurotuseryngii was found to have the highest total glucan concentrations and the highest α-glucans proportion. We also found that the stalks (stipe) of the fruit body contained higher glucan content then the caps (pileus). Since mushrooms respond markedly to changes in environmental and growth conditions, we developed cultivation methods aiming to increase the levels of α and β-glucans. Using olive mill solid waste (OMSW) from three-phase olive mills in the cultivation substrate. We were able to enrich the levels mainly of α-glucans. Maximal total glucan concentrations were enhanced up to twice when the growth substrate contained 80% of OMSW compared to no OMSW. Taking together this study demonstrate that Pleurotuseryngii can serve as a potential rich source of glucans for nutritional and medicinal applications and that glucan content in mushroom fruiting bodies can be further enriched by applying OMSW into the cultivation substrate. We then compared the immune-modulating activity of glucans extracted from P. ostreatus and P. eryngii on phagocytosis of peripheral blood neutrophils, and superoxide release from HL-60 cells. The results suggest that the anti-inflammatory properties of these glucans are partially mediated through modulation of neutrophileffector functions (P. eryngiiwas more effective). Additionally, both glucans dose-dependently competed for the anti-Dectin-1 and anti-CR3 antibody binding. We then tested the putative anti-inflammatory effects of the extracted glucans in inflammatory bowel disease (IBD) using the dextran sulfate sodium (DSS)–induced model in mice. The clinical symptoms of IBD were efficiently relieved by the treatment with two different doses of the glucan from both fungi. Glucan fractions, from either P. ostreatus or P. eryngii, markedly prevented TNF-α mediated inflammation in the DSS–induced inflamed intestine. These results suggest that there are variations in glucan preparations from different fungi in their anti-inflammatory ability. In our next study, we tested the effect of glucans on lipopolysaccharide (LPS)-induced production of TNF-α. We demonstrated that glucan extracts are more effective than mill mushroom preparations. Additionally, the effectiveness of stalk-derived glucans were slightly more pronounced than of caps. Cap and stalk glucans from mill or isolated glucan competed dose-dependently with anti-Dectin-and anti-CR-3 antibodies, indicating that they contain β-glucans recognized by these receptors. Using the dextran sulfate sodium (DSS)-inflammatory bowel disease mice model, intestinal inflammatory response to the mill preparations was measured and compared to extracted glucan fractions from caps and stalks. We found that mill and glucan extracts were very effective in downregulatingIFN-γ and MIP-2 levels and that stalk-derived preparations were more effective than from caps. The tested glucans were equally effective in regulating the number of CD14/CD16 monocytes and upregulating the levels of fecal-released IgA to almost normal levels. In conclusion, the most effective glucans in ameliorating some IBD-inflammatory associated symptoms induced by DSS treatment in mice were glucan extracts prepared from the stalk of P. eryngii. These spatial distinctions may be helpful in selecting more effective specific anti-inflammatory mushrooms-derived glucans. We additionally tested the effect of glucans on lipopolysaccharide-induced production of TNF-α, which demonstrated stalk-derived glucans were more effective than of caps-derived glucans. Isolated glucans competed with anti-Dectin-1 and anti-CR3 antibodies, indicating that they contain β-glucans recognized by these receptors. In conclusion, the most effective glucans in ameliorating IBD-associated symptoms induced by DSS treatment in mice were glucan extracts prepared from the stalk of P. eryngii grown at higher concentrations of OMSW. We conclude that these stress-induced growing conditions may be helpful in selecting more effective glucans derived from edible mushrooms. Based on the findings that we could enhance glucan content in Pleurotuseryngii following cultivation of the mushrooms on a substrate containing different concentrations of olive mill solid waste (OMSW) and that these changes are directly related to the content of OMSW in the growing substrate we tested the extracted glucans in several models. Using dextran sulfate sodium (DSS)–inflammatory bowel disease (IBD) mice model, we measured the colonic inflammatory response to the different glucan preparations. We found that the histology damaging score (HDS) resulting from DSS treatment reach a value of 11.8 ± 2.3 were efficiently downregulated by treatment with the fungal extracted glucans, glucans extracted from stalks cultivated at 20% OMSWdownregulated to a HDS value of 6.4 ± 0.5 and at 80% OMSW showed the strongest effects (5.5 ± 0.6). Similar downregulatory effects were obtained for expression of various intestinal cytokines. All tested glucans were equally effective in regulating the number of CD14/CD16 monocytes from 18.2 ± 2.7 % for DSS to 6.4 ± 2.0 for DSS +glucans extracted from stalks cultivated at 50% OMSW. We finally tested glucans extracted from Pleurotuseryngii grown on a substrate containing increasing concentrations of olive mill solid waste (OMSW) contain greater glucan concentrations as a function of OMSW content. Treatment of rat Intestinal epithelial cells (IEC-6) transiently transfected with Nf-κB fused to luciferase demonstrated that glucans extracted from P. eryngii stalks grown on 80% OMSWdownregulatedTNF-α activation. Glucans from mushrooms grown on 80% OMSW exerted the most significant reducing activity of nitric oxide production in lipopolysaccharide (LPS) treated J774A.1 murine macrophages. The isolated glucans were tested in vivo using the Dextran Sodium Sulfate (DSS) induced colitis in C57Bl/6 mice and found to reduce the histology damaging score resulting from DSS treatment. Expression of various intestinal cytokines were efficiently downregulated by treatment with the fungal extracted glucans. We conclude that the stress-induced growing conditions exerted by OMSW induces production of more effective anti-inflammatory glucans in P. eryngii stalks.
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Droby, Samir, Joseph W. Eckert, Shulamit Manulis und Rajesh K. Mehra. Ecology, Population Dynamics and Genetic Diversity of Epiphytic Yeast Antagonists of Postharvest Diseases of Fruits. United States Department of Agriculture, Oktober 1994. http://dx.doi.org/10.32747/1994.7568777.bard.
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One of the emerging technologies is the use of microbial agents for the control of postharvest diseases of fruits and vegetables. A number of antagonistic microorganisms have been discovered which have the potential to effectively control postharvest diseases. Some of this technology has been patented and commercial products such as AspireTM (Ecogen Corporatin, Langhorne, PA, USA), Biosave 10TM and Biosave 11TM (Ecoscience Inc., Worchester, MA, USA) have been registered for commercial use. The principal investigator of this project was involved in developing the yeast-based biofungicide-AspireTM and testing its efficacy under commercial conditions. This research project was initiated to fill the gap between the knowledge available on development and commercial implementation of yeast biocontrol agents and basic understanding of various aspects related to introducing yeast antagonists to fruit surfaces, along with verification of population genetics. The main objectives of this study were: Study ecology, population dynamics and genetic diversity of the yeast antagonists Candida guilliermondii, C. oleophila, and Debaryomyces hansenii, and study the effect of preharvest application of the yeast antagonist C. oleophila naturally occurring epiphytic microbial population and on the development of postharvest diseases of citrus fruit during storage. Our findings, which were detailed in several publications, have shown that an epiphytic yeast population of grapefruit able to grow under high osmotic conditions and a wide range of temperatures was isolated and characterized for its biocontrol activity against green mold decay caused by Penicillium digitatum. Techniques based on random amplified polymorphic DNA (RAPD) and arbitrary primed polymerase chain reaction (ap-PCR), as well as homologies between sequences of the rDNA internal transcribed spacers (ITS) and 5.8S gene, were used to characterize the composition of the yeast population and to determine the genetic relationship among predominant yeast species. Epiphytic yeasts exhibiting the highest biocontrol activity against P. digitatum on grapefruit were identified as Candida guilliermondii, C. oleophila, C. sake, and Debaryomyces hansenii, while C. guilliermondii was the most predominant species. RAPD and ap-PCR analysis of the osmotolerant yeast population showed two different, major groups. The sequences of the ITS regions and the 5.8S gene of the yeast isolates, previously identified as belonging to different species, were found to be identical. Following the need to develop a genetically marked strain of the yeast C. oleophila, to be used in population dynamics studies, a transformation system for the yeast was developed. Histidine auxotrophy of C. oloephila produced using ethyl methanesulfonate were transformed with plasmids containing HIS3, HIS4 and HIS5 genes from Saccharomyces cerevisiae. In one mutant histidin auxotrophy was complemented by the HIS5 gene of S. cerevisiae is functionally homologous to the HIS5 gene in V. oleophila. Southern blot analysis showed that the plasmid containing the S. cerevisiae HIS5 gene was integrated at a different location every C. oleophila HIS+ transformant. There were no detectable physiological differences between C. oleophila strain I-182 and the transformants. The biological control ability of C. oleophila was not affected by the transformation. A genetically marked (with b-glucuronidase gene) transformant of C. oleophila colonized wounds on orange fruits and its population increased under field conditions. Effect of preharvest application of the yeast C. oleophila on population dynamics of epiphytic microbial population on wounded and unwounded grapefruit surface in the orchard and after harvest was also studied. In addition, the effect of preharvest application of the yeast C. oleophila on the development of postharvest decay was evaluated. Population studies conducted in the orchard showed that in control, non-treated fruit, colonization of wounded and unwounded grapefruit surface by naturally occurring filamentous fungi did not vary throughout the incubation period on the tree. On the other hand, colonization of intact and wounded fruit surface by naturally occurring yeasts was different. Yeasts colonized wounded surface rapidly and increased in numbers to about two orders of magnitude as compared to unwounded surface. On fruit treated with the yeast and kept on the tree, a different picture of fungal and yeast population had emerged. The detected fungal population on the yeast-treated intact surface was dramatically reduced and in treated wounds no fungi was detected. Yeast population on intact surface was relatively high immediately after the application of AspireTM and decreased to than 70% of that detected initially. In wounds, yeast population increased from 2.5 x 104 to about 4x106 after 72 hours of incubation at 20oC. Results of tests conducted to evaluate the effect of preharvest application of AspireTM on the development of postharvest decay indicated the validity of the approach.
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Xu, Jin-Rong, und Amir Sharon. Comparative studies of fungal pathogeneses in two hemibiotrophs: Magnaporthe grisea and Colletotrichum gloeosporioides. United States Department of Agriculture, Mai 2008. http://dx.doi.org/10.32747/2008.7695585.bard.
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Plant pathogenic fungi have various life styles and different plant infection strategies. Hemibiotrophs like Magnaporthe grisea and Colletotrichum species develop specialized structures during plant infection. The goal of this study was to identify, characterize, and compare genes required for plant infection in M. grisea and C. gloeosporioides. Specific objectives are to: 1) further characterize genes identified in the preliminary studies of C. gloeosporioides and M. grisea;2) identify and characterize additional fungal genes tagged by GFP; and 3) identify in planta growth and appressorium-specific genes by subtractive hybridization and transcript profiling by the LongSAGE method. In this study, the PI and Co-PI collaborated closely on studies in M. grisea and C. gloeosporioides. In M. grisea, REMI and ATMT were used to transform the wildtype with promoter-less EGFP constructs. A total of 28 mutants defective in different plant infection processes or expressing EGFP during plant infection were identified. Genes disrupted in five selected mutants have been identified, including MG03295 that encodes a putative Rho GTPase. In transformant L1320, the transforming vector was inserted in the MIRI gene that encodes a nuclear protein. The expression of MIRI was highly induced during infection. Deletion and site-directed mutagenesis analyses were used to identify the promoter regions and elements that were essential for induced in planta expression of MIRI. This was the first detailed characterization of the promoter of an in planta gene in M. grisea and the MIRI promoter can be used to monitor infectious growth. In addition, the Agilent whole-genome array of M. grisea was used for microarray analyses with RNA samples from appressoria formed by the wild-type shain and the pmkl and mstl2 mutants. Over 200 genes were downregulated in the mst I 2 and pmkl mutants. Some of them are putative transcription factors that may regulate appressorium formation and infectious hyphal growth. In C. gloeosporioides, various REMI mutants showing different pathogenic behavior were identified and characterized. Mutants N3736 had a single insertion and was hyper-virulent. The gene disrupted in mutant3736 (named CgFMOI) encodes a FAD-dependent monooxygenase. Expression analyses linked the expression of the CgFMOI gene with the necrotrophic phase of fungal infection, and also suggest that expression of CgFMOl is unnecessary for the first stages of infection and for biotrophy establishment. All CgFMOl-silenced mutants had reduced virulence. In REMI mutant N159, the tagged gene encodes a putative copper transporter that is homologue of S. cerevisiae CTR2. In yeast, Ctr2 is a vacuolar transporter for moving copper from the vacuole to the cytoplasm. The gene was therefore termed CgCTR2. In addition to characterization of CgCTR2, we also conducted comparative analyses in M. grisea. The M. grisea CgCTR-2 homolog was isolated, knockout strains were generated and characterized and the M. grisea was used to complement the Nl 59 C. gloeosporioides mutant. Overall, we have accomplished most of proposed experiments and are in the process of organizing and publishing other data generated in this project. For objective 3, we used the microarray analysis approach. Several genes identified in this study are novel fungal virulence factors. They have the potential to be used as targets for developing more specific or effective fungicides. In the long run, comparative studies of fungal genes, such as our CgCTR2 work, may lead to better disease control strategies.
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Rodriguez, Russell J., und Stanley Freeman. Gene Expression Patterns in Plants Colonized with Pathogenic and Non-pathogenic Gene Disruption Mutants of Colletotrichum. United States Department of Agriculture, Februar 2009. http://dx.doi.org/10.32747/2009.7592112.bard.
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Fungal plant pathogens are responsible for extensive annual crop and revenue losses throughout the world. To better understand why fungi cause diseases, we performed gene-disruption mutagenesis on several pathogenic Colletotrichum species and demonstrated that pathogenic isolates can be converted to symbionts expressing non-pathogenic lifestyles. One group of nonpathogenic mutants confer disease protection against pathogenic species of Col!etotrichum, Fusarium and Phytophthora; drought tolerance; and growth enhancement to host plants. These mutants have been defined as mutualists and disease resistance correlates to a decrease in the time required for hosts to activate defense systems when exposed to virulent fungi. A second group of non-pathogenic mutants did not confer disease resistance and were classified as commensals. In addition, we have demonstrated that wildtype pathogenic Colletotrichum species can express non-pathogenic lifestyles, including mutualism, on plants they colonize asymptomatically. We have been using wildtype and isogenic gene disruption mutants to characterize gene expression patterns in plants colonized with a pathogen, mutualist or commensal. The US group is contrasting genes expressed during colonization by mutuahstic and commensal mutants of C. magna and a pathogenic wildtype C. coccodes on tomato. The Israeli group is characterizing genes expressed during asymptomatic colonization of tomato by wildtype C. acutatum and a non-pathogenic mutant.To accomplish this we have been utilizing suppressive subtraction hybridization, microarray and sequencing strategies. The expected contribution of this research to agriculture in the US and Israel is: 1) understanding how pathogens colonize certain hosts asymptomatic ally will shed light on the ecology of plant pathogens which has been described as a fundamental deficiency in plant pathology; 2) identifying genes involved in symbiotically conferred disease resistance will help explain why and how pathogens cause disease, and may identify new candidate targets for developing genetically modified disease resistant crop plants.