Auswahl der wissenschaftlichen Literatur zum Thema „STP analysis“

The whole genome duplication (WGD) event is an intriguing mechanism from an evolutionary perspective. Such an event may be the source of new genes, functions or species. Traces of WGD event have been detected in the genome of all four eukaryotic kingdoms: plants, animals, fungi and protists. In fungi, an ancestor of Saccharomyces cerevisiae underwent an event of WGD, about 100 million years ago, after diverging from the Kluyveromyces lineage. In S. cerevisiae, only ten percent of the resulting duplicated genes survived as duplicates. In particular, some of these duplicates encodes for transcription factors in several nutrient sensing pathways. <p>The main subject of this thesis’s work is the external amino acid sensing system in S. cerevisiae. The detection of extracellular amino acids in yeast begins with a transporter homologue devoid of any uptake activity, the Ssy1 sensor. The binding of extracellular amino acids to Ssy1 leads to the successive activation of Ptr3 and the Ssy5 endoprotease. This endoporotease catalyses the processing of two transcriptions factors: Stp1 and Stp2. The Stp factors, released from their N-terminal cytoplasmic-anchored domains, are then translocated into the nucleus, where they activate the transcription of several amino acid permease genes (e.g. AGP1 and DIP5). Starting this work, the Stp factors were considered as functionally redundant. <p> We first determined that the STP1 and STP2 genes derivate from the event of WGD. The conservation of these two genes in S. cerevisiae was accompanied by a functional divergence of their products at several levels: processing sensibility, transcriptional activation capacity, target genes, cellular abundance level and stability. The Stp2 factor with its high abundance in the cells and its higher Ssy5-processing sensibility is specialized towards induction of the AGP1 gene when the external amino acid signaling is weakly stimulated. Under strong stimulation conditions, the amino acids induce cleavage-triggered destabilization of Stp2 through the proteasomal pathway and the induction of AGP1 is mediated mainly by the Stp1 transcription factor. Unlike Stp2, the Stp1 factor is characterized by its high transcriptional activation capacity and weaker sensitivity towards Ssy5-processing. The Stp factors differ also by their genetic targets. Indeed, only Stp2 regulates the expression of DIP5. Finally, we determined that the processing sensibility and the transcriptional activation capacity of each Stp factors is directly linked to their N- and C-terminal domains, respectively. <p> The phosphorylation states and the degradation of the Stp2 factor were also examined. The event of degradation concerns only the processed forms of this factor and takes places principally in the nucleus. Some data indicate that such an event might be important to limit the activation capacity of this factor. The role of the Stp2 phosphorylation in the external amino acid signaling pathway is still unknown but this event might be important for the Stp2 degradation or its transcriptional activity. <p> The unique Stp factor from Kluyveromyces lactis (Kl-Stp), a pre-WGD species, was also studied. The Kl-Stp factor shares at least two characteristic with the S. cerevisiae Stp2 factor: high sensibility towards processing and high levels of degradation. This observation leads us to conclude to that the STP genes may have been conserved after WGD though a mechanism called neofunctionalization (one of the duplicate obtained after duplication retains the ancestral function while the other evolves to perform a novel function). <p> Finally, a new model for the external amino acid signaling pathway that brings together all the data obtained during this thesis’s work is proposed. <p><br>Doctorat en Sciences<br>info:eu-repo/semantics/nonPublished

Diplomová práce navrhuje marketingovou strategii pro výstup projektu „Collaboration in Higher Education for Digital Transformation in European Business“, zkráceně CHEDTEB. V teoretické části je vysvětlena podstata marketingové komunikace týkající se dané problematiky. Pro potřeby sestavení návrhu jsou v analytické části zkoumány výsledky již proběhlých marketingových aktivit. Vyhodnocené výsledky budou použity pro vytvoření propagace výstupu prostřednictvím modelu STP.

Thesis (M.S.)--Worcester Polytechnic Institute.<br>Keywords: TCP; RED; satellite; PEP; STP; performance enhancing proxy; segment caching; IP-ABR; Internet; bandwidth reservation; IP-VBR; congestion avoidance; bandwidth sharing. Includes bibliographical references (p. 98-99).

This report is a part of a larger project with the aim of developing a proof-of-concept prototype. The project was started on the initiative of Company X and is a collaboration between them and the University of Borås. Company X are a logistics consultant firm specialized in warehousing operations. Through this project Company X hope to strengthen their standing on the market and offer their customers new and innovative solutions. The purpose of this project is to, with the assistance of AI, calculate in what cluster within a warehouse a product should be placed in order to create more efficient picking routes and to research the market in order to evaluate the business potential for this kind of service. This specific report aims to conduct a market analysis for the potential service. It will do so by conducting interviews and creating a theoretical framework consisting of relevant literature. When performing the market research and analysis of the potential IT-service there are a few limitations caused by a number of factors that could potentially affect the significance of the end result. The service is not yet developed and cannot be described in detail but only as an idea, which could make it difficult for the interview respondents to fully understand the service. The report will also be limited for certain practical reasons such as the Covid-19 pandemic. Because of this, the report has not had the opportunity to conduct the interviews with potential customers on site but have been conducted online, via link. The theoretical framework has two different main focus areas. The first area addresses basic warehouse logistics and difficulties in selling high technology products, while the second area includes marketing strategies and strategic marketing planning. The market analysis will consist of a SWOT-analysis and an STP-analysis. The report has concluded that the service has potential and suggests that company type B is the best suited potential customer. Personal and demand driven sales are also considered helpful factors when selling high-tech products and solutions.<br>Detta arbete är en del av ett större projekt med syftet att utveckla en proof-of-concept prototyp. Projektet, som är ett samarbete mellan ”Företag X” och Högskolan i Borås, startades på initiativ av Företag X. Företaget är specialiserat på logistiklösningar och lagerverksamhet. Förhoppningen med projektet från Företag X perspektiv är att stärka sin marknadsposition och kunna erbjuda sina kunder nya och innovativa lösningar. Projektets syfte är med hjälp av AI beräkna i vilket kluster inom ett lager en produkt ska placeras för att skapa effektivare plockrundor samt att undersöka marknaden för att kunna utvärdera affärspotentialen för denna typ av tjänst. Detta arbete syftar till att genomföra en marknadsanalys för den potentiella tjänsten som underlag för forskningsprojektet. Marknadsanalysen baseras på kvalitativa intervjuer med personer utvalda genom ett experturval samt ett teoretiskt ramverk. Marknadsanalysen har vissa begränsningar som potentiellt kan påverka resultatet. Bland annat det faktum att tjänsten inte är utvecklad än och därför bara kan beskrivas som idé vilket kan bidra till svårigheter för intervjurespondenterna att förstå tjänsten fullt ut. Även Covid-19 pandemin anses vara en begränsning rent praktiskt då intervjuerna inte har kunnat ske på plats utan har fått ske digitalt istället. Det teoretiska ramverket har två huvudfokus, det ena berör lagerlogistik och svårigheter med att sälja högteknologiska produkter medan det andra behandlar marknadsföringsstrategier och strategisk marknadsplanering. Marknadsanalysen består av en SWOT-analys och en STP-analys. Arbetet har kommit fram till att tjänsten har potential och föreslår företagstyp B som den mest optimala kunden att rikta försäljning av denna typ av tjänster mot. Personlig och efterfrågestyrd försäljning anses vara viktiga aspekter av försäljning av högteknologiska produkter och tjänster.

This work shows and implements possibilities of OSI Layer-2 Network Topology Discovery based on information from Cisco Discovery Protocol, Spanning Tree Protocol and Switches' Filtering Databases<br>Diese Arbeit zeigt und implementiert Möglichkeiten zur OSI Layer-2 Netzwerk Topologie Erkennung basierend auf Informationen des Cisco Discovery Protocol, Spanning Tree Protocol und Switch Filter Datenbanken

This Diploma Thesis deals with problems of marketing strategy proposal for the company ZDT spol. s.r.o. – Agriculture and transport machinery Ltd. A marketing strategy proposal based on theoretical knowledge, analysis of the company’s current condition and questionnaire survey is introduced. This proposal should lead to strengthening our position on domestic market, gaining new market outlet and as a result of this to higher sales and profits.

Salmonella enterica serovar Typhimurium genome encodes 13 fimbrial operons. Most of the fimbriae encoded by these operons are not produced under laboratory conditions but are likely to be synthesized in vivo. We used an in vivo expression technology (IVET) strategy to identify four fimbrial operons, agf, saf, sti, and stc that are expressed in the spleen. When any three of these operons were deleted, the strain retained wild-type virulence. However, when all four operons were deleted, the resulting strain was completely attenuated, indicating that these four fimbriae play functionally redundant roles critical for virulence. In mice, oral doses of as low as 1 x 10(5) CFU of the strain with four fimbrial operons deleted provided 100% protection against challenge with 1 x 10(9) CFU of wild-type S. Typhimurium. We also examined the possible effect of these fimbriae on the ability of a Salmonella vaccine strain to deliver a guest antigen. We modified one of our established attenuated vaccine strains, chi 9088, to delete three fimbrial operons while the fourth operon was constitutively expressed. Each derivative was modified to express the Streptococcus pneumoniae antigen PspA. Strains that constitutively expressed saf or stc elicited a strong Th1 response with significantly greater levels of anti-PspA serum IgG and greater protective efficacy than strains carrying saf or stc deletions. The isogenic strain in which all four operons were deleted generated the lowest anti-PspA levels and did not protect against challenge with virulent S. pneumoniae. Our results indicate that these fimbriae play important roles, as yet not understood, in Salmonella virulence and immunogenicity. IMPORTANCE Salmonella enterica is the leading cause of bacterial food-borne infection in the United States. S. Typhimurium is capable of producing up to 13 distinct surface structures called fimbriae that presumably mediate its adherence to surfaces. The roles of most of these fimbriae in disease are unknown. Identifying fimbriae produced during infection will provide important insights into how these bacterial structures contribute to disease and potentially induce protective immunity to Salmonella infection. We identified four fimbriae that are produced during infection. Deletion of all four of these fimbriae results in a significant reduction in virulence. We explored ways in which the expression of these fimbriae may be exploited for use in recombinant Salmonella vaccine strains and found that production of Saf and Stc fimbriae are important for generating a strong immune response against a vectored antigen. This work provides new insight into the role of fimbriae in disease and their potential for improving the efficacy of Salmonella-based vaccines.

This thesis report presents a method to analyze the communication architecture for the Intelligent Transportation Systems (ITS). The report also includes a case study on ASN.1 notation and analysis of its encoding rules. Included in the report is also: (i) accompanying instruction on how to use ASN.1 compilers to produce the C/C++ message encoder/decoder, and (ii) analysis of Non-IP communications of Communication Access for Land Mobiles (CALM-FAST) protocol stack in ITS. The thesis is a part of the research project entitled “SCOOP”, a joint project between SCANIA CV AB and KTH. The purpose of this thesis is to contribute to the ultimate goal, which is to equip a vehicle with necessary hardware and software technology to provide a platooning behavior in the GCDC 2011 competition. This goal is achieved by the means of wireless communication system for both vehicle to vehicle and vehicle to road side units communications in the platoon. Overall, this thesis introduces the important usage of ASN.1 in implementation of cut-edge telecommunication systems especially in V2V and V2I communication; and clarifies the CALM-FAST protocol stack in mobile nodes.<br>Kartlägga CALM-FAST protokollet och hur det användes tillsammans med den i tävlingen GCDC 2011 fastslanga kommunikationsprotokollet. GCDC var ett tävling i kooperativ körning arrangerad och initierad av Hollänska TNO och gick ut på att få fordon att agera tillsammans beserat på information sänt via WLAN 802.11p. ASN.1 användes och ingick i analysen.

The present work introduces a methodology to obtain a discrete time state space representation of an electrical network using the nodal [G] matrix of the Electromagnetic Transients Program (EMTP) solution. This is the first time the connection between the EMTP nodal analysis solution and a corresponding state-space formulation is presented. Compared to conventional state space solutions, the nodal EMTP solution is computationally much more efficient. Compared to the phasor solutions used in transient stability analysis, the proposed approach captures a much wider range of eigenvalues and system operating states. A fundamental advantage of extracting the system eigenvalues directly from the EMTP solution is the ability of the EMTP to follow the characteristics of nonlinearities. The system's trajectory can be accurately traced and the calculated eigenvalues and eigenvectors correctly represent the system's instantaneous dynamics. In addition, the algorithm can be used as a tool to identify network partitioning subsystems suitable for real-time hybrid power system simulator environments, including the implementation of multi-time scale solutions. The proposed technique can be implemented as an extension to any EMTP-based simulator. Within our UBC research group, it is aimed at extending the capabilities of our real-time PC-cluster Object Virtual Network Integrator (OVNI) simulator.

IP Multimedia Subsystem (IMS) is the next generation of telecommunication systems. The system is based on an IP network and uses technologies from the Internet. The IMS system is designed to evolve from a telephone system into a general information and communication system. It is meant to include television, Video on Demand (VoD), interactive services etc, etc. It is designed to simplify the implementation of newservices in telecom networks. This report investigates security aspects of VoD services when merging an IP Television (IPTV) system with IMS. The investigation covers security functions in IMS, transitionsolutions for authentication of the Set-Top-Box (STB) in IMS, and identifies problems inthe integration of IPTV and IMS. The report concludes that IMS has good solid Authentication, Authorization, and Accounting (AAA) functions that will provide security and billing functionality. One problemis found in the media control between the STB and the streaming server. This interface lacked specification at the time of investigation, and some problems have been identified. These problems have to be solved before a system can be brought into service and beregarded as secure.