Zeitschriftenartikel zum Thema „Sládka“

Český strukturalismus v diskusi. Toim. Ondřej Sládek. Brno: Host, Strukturalistická knihovna 16. 2014. 394 s. Strukturalistická knihovna (”Strukturalistinen kirjasto”) on Tšekin brnolaisen Host-kustantamon korkeatasoinen sarja, jossa on ilmestynyt jo kohta 20 strukturalismi-aiheista teosta. Niihin kuuluu muun muassa ranskalaisen strukturalismin piiriin lukeutuvia teoksia sekä strukturalismin kanssa keskustelevien saksalaisten, amerikkalaisten, tarttolaisten, puolalaisten ja muiden ajattelijoiden ja tutkijoiden tekstejä. Valtaosa julkaisuista kuitenkin viittaa tai suorastaan kuuluu tšekkiläisen tai oikeastaan prahalaisen strukturalismin traditioon.

Czech hop varieties are evaluated as part of maintenance breeding. Every year, this evaluation includes ten mother plants of each hop variety. Yield is determined in kilograms of fresh hops per plant. The t-test is used to determine significance. Results from the years 2014–2019 show that Gaia and Kazbek have the highest yield (3.17 kg/plant and 3.05 kg/plant, respectively). Their yield is higher than that of the Harmonie, Agnus, Bohemie, Premiant, Sládek, Boomerang and Saaz varieties. Saaz has the lowest hop yield, i.e. 1.85 kg/plant. Bor, Saaz Late, Saaz and Sládek show the lowest variability, which ranges between 20.56 and 20.58%. Gaia has the highest variability (34.33%). Gaia also has the highest alpha acid content (12.30%) with a probability of 95% to 99%. Vital and Boomerang have a higher alpha acid content than other Czech hop varieties (11.09% and 10.81%, respectively) with a probability of 99%. Saaz Late (3.42%) and Saaz (3.06%) have the lowest alpha acid content with a probability of 95% to 99%. The variability of alpha acid content is considerably lower than the hop yield variability. Rubín has the lowest year-on-year variability of all Czech varieties (4.81%). Vital and Gaia have a very low variability (5.63% and 5.72%, respectively). A higher variability of alpha acid content was found in Saaz Late, Premiant, Agnus, Saaz, Kazbek, Bor and Harmonie, ranging between 8.01% and 10.21%.

Article first published in: DEB MITRAJIT, ROYCHOUDHURY SHUBHADEEP, BHATTACHARJEE PARIMAL C., SHARMA INDU, NAUTIYAL SUNIL, SLÁMA PETR. 2019. Distribution of Western Hoolock Gibbons and Nutritional Status of Food Plants in Cachar District of Assam, India: Reaching out to Local Communities for Conservation. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(1): 25–39. https://doi.org/10.11118/actaun201967010025.

This study quantifies the effects of weather conditions, irrigation, and plant age on yield and alpha-acids content of Czech hop cultivars Saaz, Sládek, Premiant and Agnus in a 25-year long period, i.e., from 1993 to 2018. The yields of Czech hop cultivars were increasing for the first three years of plant age until they stabilized and then started to decline slightly for 15 to 20 years until they reached the optimal time for replanting. The highest alpha-acids content in hop cones was achieved in the first year of cultivation, followed by a logarithmic decline in the upcoming years. Rainfall was the most significant factor that positively influenced the yield of Saaz hops with correlations of r = 0.59 and 0.61 (P < 0.01) for total seasonal rainfall (April–August), 0.65 (P < 0.001) and 0.60 (P < 0.01) for daily rainfall above 3 mm, 0.37 (P < 0.05) and 0.58 (P < 0.01) for rainfall in May and 0.50 (P < 0.01) and 0.32 (P < 0.05) in July in the Saaz region and the Stekník farm, respectively. The yield of cultivars Sládek, Premiant and Agnus was not statistically influenced by the amount of precipitation, but there was a positive effect of irrigation level on yield increase with correlations 0.58 (P < 0.01), 0.55 and 0.49 (P < 0.05), respectively. High air temperatures during summer were the most significant factor that negatively influenced the alpha-acids content with the correlations ranging from –0.56 to –0.83. However, cv. Agnus showed a stable weather-independent alpha-acids content.

HOP (Humulus lupulus L.) Breeding Aimed at High Contents of Desmethylxanthohumol (DMX) Three female plants (cultivars Agnus, Columbus and Admiral) and two male genotypes (99/14 and 00/6) were chosen for diallel breeding. The highest content of desmethylxanthohumol (DMX) was determined in the progenies of "Agnus x male 99/14 and 00/6", resp. However, the differences between the progenies were not statistically significant. Within combination breeding the highest content of DMX was found in genotypes originating from cultivar Vital. These progenies show higher contents of DMX than the other ones with 99% probability. In the second part of the breeding process aimed at high content of DMX we tested the hop gene pool as well as contemporary breeding material. In currently cultivated bitter hop cultivars the contents of DMX are low from 0.14 to 0.18% (Agnus, Magnum, Sládek, Dunav, Pioneer, etc.). New genotype with very high content of DMX (0.37, resp. 0.27%). In 2008 the first Czech hop cultivar (Vital) for pharmaceutical utilization was released. This variety shows high content of pharmaceutically needed compound, DMX (0.3-0.4% w/w). In comparison with other bitter cultivars of hops this content is 2-3xhigher.

In vitro meristem cultures have been used for the production of hop (Humulus lupulus L.) virus-free rootstocks worldwide, because multipropagation is considered to preserve the genetic stability of the produced plantlet. Nevertheless, in vitro tissue cultures can cause genetic and epigenetic changes. Therefore, we studied the genetic and epigenetic variability of Saaz Osvald’s clones, Sládek and Premiant cultivars on the DNA methylation level by methylation-sensitive amplification polymorphism (MSAP). In vitro propagated plants, acclimatised glasshouse rootstocks as well as derived mericlones and control plants under field conditions were used for the analyses. A total of 346 clearly and highly reproducible amplified products were detected in the MSAP analyses within the studied hop plants. We found 16 polymorphic products (4.6% of products) and 64 products with methylation changes (18.5% of products) in the analyses. The demethylation events were comparable to the de novo methylation events. Most demethylation changes were found in the in vitro plants, but only a few of them were found in the derived mericlones under field conditions. In contrast, the de novo methylation changes persisted in the acclimatised plants under glasshouse or field conditions. A hierarchical cluster analysis was used for the evaluation of the molecular genetic variability within the individual samples. The dendrogram showed that the individual samples of the same variety, more or less, clustered together. Because the methylation status varied during the virus-free rootstock production process, we suppose that de/methylation process is a natural tool of epigenetics and evolution in vegetatively propagated plants.

Abstract The ambition of the survey study, which maps the work of Slovak directors in Czech opera theatres after 1993, is to identify the number of Slovak creators in the opera-theatre discourse of the very closely connected countries in terms of culture and history while at the same time adding the professional biographies of Slovak artists – who are little known and reflected upon in their homeland – and parts of their works. The author concludes that the split of the Czechoslovak Republic and the subsequent creation of separate Czech and Slovak Republics did not have an adverse effect on the mutual contacts of our opera cultures. At present, we even enjoy intensified co-operation in both directions. The nonjudgmental attitude of Czech theatres towards the influence of Slovak film directors in the Czech Republic is clear: not only credible creators (Marián Chudovský), but also representatives of the younger generation of opera directors (Andrea Hlinková) and renowned drama directors with previous opera experience (Martin Huba, Roman Polák), as well as creators who had not yet worked on the opera scene at home (Martin Čičvák, Sláva Daubnerová) were presented with an opportunity to contribute. Despite the fact that their works represented the enrichment of the Czech opera-theatre, the Slovak director with the most significant contribution to the Czech opera theatre remains Jozef Bednárik, even two decades later.

This review describes the over half-centennial history of research on insect juvenile hormone (JH) as well as its natural and synthetic bioanalogues (JHA or juvenoids).The leading theories of insect hormone action in growth and metamorphosis were created more than 50 years ago by the pioneers of insect endocrinology, V. B. Wigglesworth, C. M. Williams, V. J. A. Novák, H. Piepho, H. A. Schneiderman and L. I. Gilbert. There are two principal categories of hormones released from the central neuroendocrine system (neurosecretory cells of the brain, corpora cardiaca, corpora allata) that regulate insect growth and metamorphosis. The first is a complex set of neurohormones (neuropeptides) originating in the neurosecretory cells of the insect brain, which are released from the neurohaemal organs, the corpora cardiaca. These neuropeptides are responsible for stimulation of various developmental events, such as the release of the activation hormone, AH. The second category of centrally produced hormones in insects is the morphogenesis inhibiting hormone, or juvenile hormone (JH), produced by the associated endocrine glands, the corpora allata. JH is responsible for induction of the somatic larval growth in young instar larvae and stimulation of reproduction in the feeding adult stages.Wigglesworth (1935) first described JH as an inhibitory hormone; Williams (1957) discovered its active extracts. Sláma (1961) discovered the hormonomimetic or pseudojuvenile effects of various lipid extracts and free fatty acids. In addition to lipid extracts with JH activity, a phenomenon found in various human organs, microorganisms and plants, JH-mimetic materials were found in American paper products in 1964. The source of the so-called “paper factor” was the wood of the Canadian balsam fir. The potential use of these and other analogues of JH as nontoxic, selectively acting “third generation pesticides” stimulated an enormous boom of activity among industrial and academic institutions all over the world, in the pursuit of synthetic JH analogues for replacement of toxic insecticides.For practical reasons, in this review the chemical structures of the synthetic juvenoids have been divided into three categories: a) natural and synthetic, predominantly terpenoid juvenoids known before 1970; b) terpenoid and nonterpenoid juvenoids synthesized and tested before 1980, and; c) predominantly nonterpenoid, polycyclic juvenoids with relatively high JH activity, found and selected for practical use after 1980. Chemical structures of several juvenoids of theoretical or practical importance, together with the essential structure-activity relationships, are outlined in several figures and tables. The total number of all juvenoids reported active in one or more insects species has been estimated to be more than 4000 compounds. A juvenoid molecule has, more or less, a similar molecular size, roughly equivalent to a chain of 15 to 17 carbon atoms, with the presence of some slightly polar functional groups and a more or less lipophilic physico-chemical properties. Beyond these similarities, there are many variations in the structural types of juvenoids, including, derivatives of acyclic terpenoids, arylterpenoids, peptides, heterocyclic and polycyclic juvenoids, phenoxyphenyl juvenoids, juvenoid carbamates, and pyridyl-derivatives.In addition to the generally known and intensively studied effects of juvenoids, such as inhibition of metamorphosis, inhibition of embryogenesis, and stimulation of ovarian growth, there are certain less remarkable and largely unexplored biological effects of juvenoids. Some of those phenomena, which are briefly described in this review, are: a) the effects of juvenoids on embryonic development (ovicidal effects); b) delayed effects of JH on metamorphosis from egg stage; c) sexually transmitted female sterility caused by juvenoid treatments of the males; d) the nonvolatile, biochemically activated juvenogen complexes, generating hormonally active juvenoids by enzymatic hydrolysis of the complex, and; e) antihormones with antijuvenile activity.There are two basic hormonal theories on the regulation of insect metamorphosis by JH that have been proposed during the past 50 years. The first is the theory of Gilbert-Riddiford, which has been widely disseminated at universities worldwide, through textbooks on insect physiology, biochemistry and endocrinology. The second, less renowned, hormonal theory of insect development is that of Novák-Sláma. Briefly, the Gilbert-Riddiford theory is based on several fundamental principles. These are: a) the brain hormone-prothoracic gland (PG) concept created more than 50 years ago and later disproved by Williams; b) the conclusions of Piepho, who suggested that a large concentration of JH would cause a single epidermal cell to develop larval patterns, pupal patterns at medium concentrations, and adult epidermal patterns at zero concentration; c) small amounts of JH are necessary in the last larval instars of endopterygote insects for preventing precocious proliferation of imaginal discs; d) metamorphosis is stimulated by PG through a small endogenous peak of ecdysteroid preceding the large prepupal one; e) ecdysteroids are released from the PG in response to superimposed prothoracicotropic hormone (PTTH) from the brain; f) the true juvenile hormone of the corpora allata is a sesquiterpenoid compound known as epoxy homofarnesoate (JH-I), isolated from the adult male abdomens of the Cecropia silkmoths, and; g) physiological functions of JH and other hormones are regulated at the peripheral level by enzymes (esterase) or genes (methoprene tolerant,Metor a Broad complex gene).The Novák-Sláma theory is based on completely different building blocks. Briefly, these are: a) the PG represent a peripheral organ which is not involved in the regulation of the moulting cycles, instead; b) the PG are a subordinated target of JH (not PTTH), they are inactive during the last larval instar and their removal does not abolish the cycles of moults; c) the PG are used to generate metabolic water during the growth of young larval instars by secreting of an adipokinetic superhormone, which stimulates total combustion of the dietary lipids; d) small, medium, or large concentrations of JH are unimportant, the hormone only needs to be present in the minimum, physiologically effective concentrations; e) an imperative condition for metamorphosis to occur is a virtual absence of JH starting from the second half of the penultimate larval instar; f) JH acts according to an “all-or-none” rule at the single cell level, and the temporal sensitivity to JH is strictly limited to a narrow period at the beginning of the moulting cycle, before the cells begin to divide; g) the corpus allatum never produces JH in a nonfeeding stage, and the sesquiterpenoid juvenoid JH-I cannot be the true JH of insects (it has very low JH activity, 100,000-fold smaller in comparison to human made peptidic juvenoids); h) the developmental cycles are stimulated exclusively by neuropeptides produced by the brain’s neurosecretory cells (AH); i) developmental stimulation by AH has nothing in common with the PTTH or PG; j) when environmental interventions in the hormonal system become obsolete, the regulation of moulting cycles becomes autonomic (hormone independent), supported by the stereotypic instructions coded on the genome; k) during the millions of years of insect evolution, the central neuroendocrine system acquired the superimposed, epigenetic ability to adapt gene functions and synchronize them with essential changes in the environment. A model based on the regulation of insect metamorphosis by simple combination of two hormones (AH, JH) of the central neuroendocrine system is outlined. A possibility that the 4000 known juvenoid molecules act as the feedback or homeostatic factors affecting permeability of the epidermal cell membranes has been suggested. Speculations about possible peptidic or proteinic nature of the corpus allatum hormone have been emphasized.

AbstractM.H.L. is a theatrical production dedicated to the first Slovak professional female director Magda Husáková-Lokvencová, which combines documentary theatre and performance. Sláva Daubnerová wrote the script and scene concept and is director and plays the sole character in the play. She portrays the private and professional life of M.H.L. in a chronologically sequenced and mosaic-like fashion. M.H.L. is portrayed as an educated, broad-minded and intelligent woman who knows her own mind. She graduated in law and then took up theatre direction. Her private and professional life represented a series of ups and downs. Initially happy at the side of her husband, Gustáv Husák—successful early on as a politician, and flourishing in the theatre, she later had to struggle to survive and make a comeback to the theatre. Performer Daubnerová, enhanced the performance by using video art and sound design as important elements in the production.